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<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T0N8</accession>
    <name>A0A8J2T0N8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_06260g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_06260g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T0N8"/>
    <dbReference type="OrthoDB" id="10251136at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19509">
        <property type="entry name" value="RecA-like_VPS4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000022">
        <property type="entry name" value="Fidgetin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000093">
        <property type="entry name" value="Fidgetin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF81">
        <property type="entry name" value="26S PROTEASOME SUBUNIT YTA6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035179635" evidence="6">
        <location>
            <begin position="30"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="454"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="202"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="155"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="264"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="274"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="310"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="348"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="376"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="703" mass="78900" checksum="40704CA8B3062863" modified="2022-05-25" version="1">MSNEKFAIPANFTLLQSLQLLYSIVASQCENYEKQTAKLELSISDLAKLYRLFDNLLAYMNDGLFRIEKYYKCEGGLADALMGHKDLKRRFEDLQILGQDIRLSRRECNNRIDVLEQIRNSQSGMIKGLFRSRGTKKCKPRFEDEKQEKAAIAAAEKVRQARKDKEMKKVEEEKQRQLELEAEKEKQMEIMIKQQLEAEWLENNAAGRPMDKRKTTTSKRSTASVGSPTIRRTERHSMYVSSPTATRTSLDDDHHHRYVGHATRSASQNSAQKTGTRSFSSPSSNGNGNGSTGTKKRYEYKKPVVRRQQIKSPRSQSNQSRATSPELVATDARGTHREKAHPPRRIQHQSPQTPSASSSPIPVQAASFERNTPVSGDEEFSDEQSDSLTDHYMDSASHGVDPLACRQIMKEIVVADEEIRWGDIAGLLNAKNSLKETVVYPFLRPDLFKGLREPIRGMLLFGPPGTGKTMIARAVATESKSTFFSISASSLLSKYLGESEKLVRALFYMAKKMAPSIIFIDEIDSLLTARSDNENESSRRVKTELLIQWSSLSSATGNGPGGETDSRVLVLAATNLPWAIDEAARRRFSRRLYIPLPEYETRLHHLKKLMSRQNNGLSDVDFQVIAEMTEGFSGSDITALAKEAAMQPIRDLGDALMDADFNNIRPVMVKDFEKAMLTIKMSVSPASLQQYQDWAAGFGSTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T148</accession>
    <name>A0A8J2T148_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cAMP-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_16974g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.11" evidence="2"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T148"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010607">
        <property type="term" value="P:negative regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010737">
        <property type="term" value="P:protein kinase A signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05580">
        <property type="entry name" value="STKc_PKA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF153">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="49"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="304"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF87898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="359" mass="41652" checksum="E42616DD87187121" modified="2022-05-25" version="1">MEYAYPRYPQTSHQPHQPSQVLPSYVPRHKSLLPQRSGISKGKYNLRDFQILRTLGTGSFGRVHLVRSVHNGRYYAMKVLKKQQVVKMKQIEHTNDERHMLRLVEHPFIIRMWGTFQDARNLFMVIDYIEGGELFSLLRKSHRFPNPVAKFYAAEVTLALDYLHSHNIIYRDLKPENILLDRNGHIKITDFGFAKEVVTVTWTLCGTPDYIAPEVITTKPYNKSVDWWSLGVLIFEMLAGYTPFYDTTAMKTYEKILQGKVTYPPFFHPDVVDLLSKLITADLTRRLGNLQSGSQDIKSHPWFSEVIWEKLLAKDIETPYEPPVVPGVGDTSLFEMYPEEHLDYGIQGEDPYGQYFGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T185</accession>
    <name>A0A8J2T185_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clathrin light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_17348g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasmic vesicle membrane</location>
            <topology evidence="1 6">Peripheral membrane protein</topology>
            <orientation evidence="1 6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Coated pit</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="6">Cytoplasmic face of coated pits and vesicles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the clathrin light chain family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T185"/>
    <dbReference type="OrthoDB" id="5512at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000996">
        <property type="entry name" value="Clathrin_L-chain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01086">
        <property type="entry name" value="Clathrin_lg_ch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0168">Coated pit</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="21"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="125" mass="14237" checksum="C2A50C89D79DD7B8" modified="2022-05-25" version="1">MSFEGENGNGAIAEWQAKRETEIAERDAADAEAKKELKEEAVKHIDDFYDNYNRKKAQQLEDVRKEAEEFQKSRDEFSSQEGTTTWDRVLQLINEDDADQVAGRDKSKFKEILQRLKGNAAAPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T228</accession>
    <name>A0A8J2T228_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_13234g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_13234g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T228"/>
    <dbReference type="OrthoDB" id="4070605at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="601"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="385"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="632"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="707" mass="79068" checksum="8C9725444F4B48B1" modified="2022-05-25" version="1">MSSDKPGGRWPHGYRPKVEVQAQNSLFLINYSDVPSETAQETVEPLARQFEVSVFIDIPTIDFYKDELTNTKLSKFNKQFKLHLLKESIYKCFTNGNEKFWGRWLKSSGDGSSCRVSIASSGSLRYVETIKFLVETVYEKLEEYIACGRLQLRFNLDTSPTSRKWFSTFLDHRLLQAGMVTFTQIGRPACEMGPSPFKEYYMTLTKKFEEIENGTGDPNCITSVVIVTNSTGVKALLTILSDRPLTNFISRESMKMLHDYALKKSHVPINQEETPLKRESSSLLNFQNQLLTSNKDKSVRIRSVSLNRRAIVVASAAAPMSPSPPRTNSMASMHQDEPKHIDEFDQRETREPGEDDIDGDDDDDNEDDENDNDEDDDGISFNVPSKLSRSGSSELLSSAMTRGRSGRIRSLSLMDPALRKPFAQNMLSLSPPLIPTDDEGSSNENARVSNIYVHDGDFYDQDGIITKRNRKRQIKLSRHSSANGLIPPEFYSRFSSPSTSASSSNTSLQNLHLAPGTFSKMAGNHGNGNAPVDSLFEKSLINKSFEESRSHNMFGSLIERLNQSSNAHNGKNDLALNFVSNKTIPLHMLDLDEEDLLMSGTNRPISSEETSDDDSRSNSTVVPMNREDESSSPDQNSPRDTTPTMTIDLTKIKLYDDPSSQPLKHNIQKPSAAEQPKPKYKKAVTLDLYGDDDVENSGGWLLGGNAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2S2</accession>
    <name>A0A8J2T2S2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_05820g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_05820g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2S2"/>
    <dbReference type="OrthoDB" id="2018507at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14037">
        <property type="entry name" value="STKc_NAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967:SF65">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE AKL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="31"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="393"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="463"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="562"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="421"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="479"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="595"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="639"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="670"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="685"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="729"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="747"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="799"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="814"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="860"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="887"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="919"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="942"/>
            <end position="956"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF88257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1001" mass="111508" checksum="871B7B803DE5096E" modified="2022-05-25" version="1">MPATSGASSLNKPAVDGYAPGTVVSVGNQQVEVLSYLAEGGFAQIYSVKYLQYIGEFKNGATAPLKPGDKACLKRVLVPDESGLSEMRNEVEVMKQLRGAKNIIQYYDSNASRRSGGQVGFEVLLLMELCSNNSLLDYMNQRLATKLTEKEILKIMYDTTLAISQMHYLPVPLIHRDIKIENVLVDSNNNFKLCDFGSASTCCPLVTTHQDIAILTQNIYVNTTPQYRSPEMIDLYRCLPINEKSDIWALGIFLYKLLFFITPFEMTGQFAILHSKYEFPANSYSSKLINLIIIMLAENPYVRPNIYQVMHHICAVIGCEVPIVDKYASGPYNFEKYTEFQTKLQNVQYQMYLLQQKKLENHGYLNSVESKLLNDLIIASFEVSGKVPAEVGRTAPLKHKSPKPKEVNLNSSDESSDQRKSVFSSRNPSRNPSGASRKNTMPSSEFDNELDANLEAESKQFAAVSMSDREPHLSPHNQKSLSSYSSGGKSLSGGPHDKSSEALSGEQATKTEASPGAMKQHKSNNPFPMMAQGYSQPTPATTAPEPIHPQEYFLDDQQSFPQAQMRAHSPEDSHFKQPAPQIPHSIPVPPAQSGLPRSNYNQNMFYGHVDSPVSAHAQSQPTSFKKVPEATQPQMRFNYQVPQTQPSSSPYYPSPMQAPAKLATPAAAPMLPPPAQQPPKPPQPSQTSQQRSRPVPPQQSQHFKPAQPASQQVEQAMPLQPAKEEVAPPENPPPIPPHPRTWQDYTVSEKPDLKQKLNRRDEKTVPFMKQRSRTDSPLLEKTPSKNLDLTYNEMDLGSDDPKPKRSTQARREAFSSTQSDMASSESIEIDLDDAKRATKRSPRTSNDKSTPAPEKLFRHLNSSSGSLRQSLDLKYQEVHFSSPDLAKQAKETKRQDNGDQGGRRGHPNNHSDSNRRHSNNSGGPSNSPPKNEMERYKNTRKNNSNSSINVSGSTGSDMRKSFAKARQSLDLEKIRREAVKGEAVIAGKRRSLFSVFRGDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3L7</accession>
    <name>A0A8J2T3L7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_13806g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_13806g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3L7"/>
    <dbReference type="OrthoDB" id="3251668at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="568"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="321"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="386"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="460"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="503"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="536"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="553"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="639" mass="69271" checksum="8C0819B7C8050D30" modified="2022-05-25" version="1">MVPQEENTLPLAPAHADKGQEAPSRREEAPGPDNHPSETRGEQCKQGLITAHIHSIKTQYDENPLLSITKPTSAPHNTPVQSPPTSPGSAAAKQNRHAPSHKGLISSLPHLPPPPLAAVSSTRGADGTTDGGTQPPNRDHVVSPTTAPAAAAGTAAAAAAGAGGAQDKTENEEDSPISIINRRNIFATSDSRCSNSSKQVAGPPTREPMRQGHGSSSSSHGMAPQSFYFDKDRSMTDPHVKHEAADQKINISSWRQGADAYTLMDGIGNAGSVPNSSGSAIGVANAVAAGSRSRSHSRSSARGSETNGPARIANGFNSSPNGVEGVVTTTGDDNDDNYVPPPPPRYINSKLDDIRSRLLLNPRTVSPRSEEDMDKTHAAAVLSNMRSSPFRLSDRPSSLSSSRPGSSSFSGKGYARPVVRIHHREEPSMEADDNAVLDDEDGEDDDEDEDENGNERIRHASTSTAVTAPTAERRKEVTWNKNGKRVSRRLSAPETRRMKNKKLKTENRDSSIRSRDSSVPRGDDIDDTYSSHEMLENEDDDEEEYEDSKPGSKGQSKKNSMGSRSRTGCWICRLRKKKCTEERPRCYNCDRLNLTCYYDVIKPDFISNPQKKQMKLDEIKKKTKEAKRNAMRKKPGANP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3P5</accession>
    <name>A0A8J2T3P5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein transport protein sec16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_06392g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Involved in the initiation of assembly of the COPII coat required for the formation of transport vesicles from the endoplasmic reticulum (ER) and the selection of cargo molecules. Also involved in autophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SEC16 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="8918678at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09233">
        <property type="entry name" value="ACE1-Sec16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024340">
        <property type="entry name" value="Sec16_CCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024298">
        <property type="entry name" value="Sec16_Sec23-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13402">
        <property type="entry name" value="RGPR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13402:SF6">
        <property type="entry name" value="SECRETORY 16, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12932">
        <property type="entry name" value="Sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12931">
        <property type="entry name" value="TPR_Sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Sec16 central conserved" evidence="10">
        <location>
            <begin position="1430"/>
            <end position="1490"/>
        </location>
    </feature>
    <feature type="domain" description="Sec16 Sec23-binding" evidence="9">
        <location>
            <begin position="1549"/>
            <end position="1834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="230"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="325"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="555"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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            <end position="1112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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            <end position="2045"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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            <end position="2134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2147"/>
            <end position="2177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2215"/>
            <end position="2241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2284"/>
            <end position="2325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2344"/>
            <end position="2452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2465"/>
            <end position="2595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="230"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="268"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="325"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="360"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="400"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="447"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="470"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="484"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="517"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="589"/>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="614"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="666"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="701"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="844"/>
            <end position="858"/>
        </location>
    </feature>
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        <location>
            <begin position="884"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="920"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="956"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="995"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1077"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1315"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1331"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1919"/>
            <end position="1945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1988"/>
            <end position="2002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="2099"/>
            <end position="2112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="2122"/>
            <end position="2134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="2147"/>
            <end position="2164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="2165"/>
            <end position="2174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="2223"/>
            <end position="2241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="2293"/>
            <end position="2305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="2314"/>
            <end position="2325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="2356"/>
            <end position="2371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="2372"/>
            <end position="2425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="2437"/>
            <end position="2446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="2527"/>
            <end position="2544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF88280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2595" mass="283356" checksum="8F837F7EAE274722" modified="2022-05-25" version="1">MTVEARKRKNQKKKLRQKEKKAAEKAASESIANFEDVEVAGIRADPLIVDRVAAEQVLPPQALKGKTNELISQTEWTRQGSPQISTEPMGQALETDAEFEYGGSRVRQNTEGNETSLIDQRSEGPVYTIFSGIQHEPIPQRALQSIEPASRLPEEAPISDVALEGSIVDIKQATETSRRISEVSPLQAVNEQDARCLSQENITKEEKSCEIKEQPKKEVLNVRASTNEVKNVNDINSNQGQHSSAADEVSERLNLSESTQLPSQASNHGEKNHKEKNSSEGEETNSKQLNGEGVDSSHADPTLRKVVPQMGLRQKLIVEDECSDNRINETHESTSGREVRSSQHCRNSDEDIVTSEDKTTLSLPSEQSPVECSELPDEPIALSEKSTETTVKSIAPSEASVATQDASAQTFEEPTENREVPAKAPAKSIGTAEEYAEISDESRRYSVASTSTLEESQGTPGESAKYAVESQAQAEQSPELSGKFPERTEKEDVVRADDRTESLLLKEKVDIVPVEMASKASSSEASKLSQEESGSFEKLEAQSVFKKDISSNELQEAEKTIQEEVSSNHSALEELSDDENQSCVYGGNLDDKRDHEQIHEDAHTSPEEVIPSGATGSKRTSVNIAGQESQSNLKTEEQTQNSFVEEYPPKSKSDREVDACIDALFDEDKGAKREPLPWEASEHVQESLNQNISESSVSVKSENEESKQLSLSDRMERPPKSDHDVAVSISNIEGGQPSPDTPETIPLPAESEDSKVIKKPQFVNESDLDAAGSVVDNQSQMVEPEDQVDQLFGVVGNQESVQMPWEAPESSSNAGEGQVSKQDETKNLSGTTAQVESLFEEEQHEPMPWEESRVREDNEAITSASDENGDKGRNVATKKFSFLDNDDDLLDDDDSYLESEEDIEEIEEVEEVEEVDEKEENEKNEHMRTVESELVEVTPKNVGNPQGVGKTMVVPFNSSTDSQGATSKYKPSNVPQAQSSFLHPRSYEAGVPPQAIQNNLSHSSASSQQPVFASQQRTILEGEKVVQKINEEKKKSDAFDFPMDLVSKKTDHVPAKPVGASRFASPPPFDLPGQAPPRSSRTNSIASLPSNPYATAATTSKNKVQPPLPPQGVVLPQNALPPGNKLPAPPAVPFPAGLQASVRSQHPLNLRTRGFSNISAEGSLSAGTIGSLPTPPHYPISPSYGHVALGKAAENKYAPASPSRVPIGNPAVDGVIGYTAPGPKVTGPPGSGPAKTGLLGGGPYAAKNATQKLPVSPPFKHVIPSVPPAAPQNLPHPSVHGKPTVNVTPNTPAEVYQPGAGVFNNVIAGPPISARQKRSTSRSHARSNSSVYTPNQSEFTSKYAPTVHPHYQNPSPPQMPMVTQNPLSSINSYMASAPSQAAARPESSQIPKPVNNDALLQHQFPLFSWSASEKVNYGIPIDNGQNAYIVGSSSSIQNFNLVNVETILFKHNFLKAFPGPLSKNKTKKKDLQKWFDDYADEFSKEEDSLNITVVSLLKLKLTENVTFKDVSRILYNSDDLLLYLSQQPAVRSRQVPSAFKLDANNQLKISAYLQTGGQNDALQLALDMKDFSMALLIGSLMGKEKWSEVVECYLAHEIELGAGDSGFLTNLFSLIFQVFIGSSKTAFQEFYVNDTKCKWALENWRMVVAAVLNNIATPVVAISSGVLEVPPVVVEFLVEYGVFLSQKGMKLPACILFMIANLPLSSSPVTPDVDVKFESVGSPESLESVLFSEVYEFCVSNPKPFPVLLPLKLYHVACLQERGLTTAASKYTDHLGSILRTLPKKEPFAIKLTHQLNDLTACIAGNSTGWLGKPKLSSVWGHLDKSFNKLIGGDDDALIKKSTEKKVFDGFTPVGSRNNSTVDLHQMPFVPYQGHSKKSSYGHSEGIIPLQPIGHSPIREPIPIENVYGGKSQPEPRNPRTSQVNLNQDTPNASQHNSSKPLSASQPPPQLRRVHTMQPIGGVLPPFMRASSESLNAAADYQEKHTSGNRLQEIPTSVISEDTSSKKRSAPRYNSTPNLLRRSSAVSDTSSAGKEGSVGTKKLADTPYGIAGEQLEETVEENDFASRSLLKSSDCEIPDDGHQLLVRKPILEAPPYLQGNESSPISTVATESSTDDDDMNNEAESTAASSNRLPGTNAVIEHNMPLTMNQPTLGDQHSGETLSQNEKRLSEKSNDTLQTAPVQSDNVLTIKAKTTDPGLGINMREKDALNSEESHIIPPVSTPNPYTVRNTTRKTSSSKMSYLPKNISQVSASTAGHSDISGMNVNGLGMFVEQSNVVSQAPAESSISSSSITNPKASSLVSQESPLAINRADPTSRFDPIKVPEEISTETFEPVIKKTPNMKAFTPLVVQPPETQYDDIVEDESEDEEDQEAIRKKEEEKIKAEEEERRESERREKEEKERREEERREEERREEEKRRKAKNDSQENSSTGWFGWLKKDPNEKKPIKAKLGNKKRNFYYDEKLKRWVNNNSTEEEKEKLSTPPPPPPVVKRMDNGPKTKPRSGSLNAAPKAVTATIAPKNPITGEPLTSQSLNVSRVGSQTPSLSPPIGNSGINLSGKKANGLDDLLNLTGSTSTTARRKKKPGRGYVNVMENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3X7</accession>
    <name>A0A8J2T3X7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BN860_16336g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_16336g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3X7"/>
    <dbReference type="OrthoDB" id="6407164at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000047">
        <property type="entry name" value="Differentiation 1 negative regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000120">
        <property type="entry name" value="Negative regulator of differentiation 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF8">
        <property type="entry name" value="RNA-BINDING PROTEIN MRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="155"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="246"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="395"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="486"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF87869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="576" mass="63254" checksum="EEFB29997B552125" modified="2022-05-25" version="1">MHSYNTSSMNQLFNSDVHFPNTNLSRSVSPEQSAYGGAPTNQLLQSRYNSVELQPQNEYPLLSYDNKTQIQFQRRPQRVSQGMNFGNLQDGESASAFSTPYLLQNNQRLMQSQPQGPQSEYALAAAAAAVANANAFMPSIPQLGNSRNFNNAPSKTVYLGNTPPTLTVKELLDHVRSGVVEDVKILPDKMCAFVSFVDESAALLFHSDAILKRLHIDGRDIKIGWGKPTPIDPVIATAVANDGATRNVYIGQLNTGPNLAAQASVDLSEPVITEEKLRLDMQEFGEIDCIKIVEDRGIAFVHFASILNAIKAVTSLATINPYYKNKKIFYGKDRCAFITKTQQHNAAQFLGVQPGMEGLVQFSDSELISNALLQQSAAAAAIATSAGGPNNLGNRTVYLGNLPKDVKVEEICNVVRGGLLQNIKVLVDRHVCFVTFIDPTAAAQFYAMSSLHGITIQKRRCRVGWGKHPGSLENALALAVSNGASRNVYVGNINFTLDNEGEKPFFTESNLRAIFQQWGEVEQINFLPERNCCFINYTNISNAILAIDKIKGHPAFKDLKINFGKDRCGNVPHQVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3Y6</accession>
    <name>A0A8J2T3Y6_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4 15">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BN860_07118g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Endoplasmic reticulum membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4 15"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3Y6"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23284">
        <property type="entry name" value="beta-trefoil_MIR_PMT2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF52">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="210"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="590"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="622"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="655"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="683"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="308"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="379"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="450"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CDF87471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="726" mass="84071" checksum="E67A43EB3587C3FD" modified="2022-05-25" version="1">MAESEKLEEKGLAQPMAAPLVNTKVKWWLRFEGILSPLAYTTLGLCLRFHDIEKNNGVVWDEAHFGKFGSYYIKHEFYHDVHPPLGKMLVALTEWLAGFDGDFDFDSNKVYPENVNYKLMRQMNAFFGVLCCPLAYWTAKNMGFNLLTTHLVALMITLEHSYITISKFILLDSMLLFFTISTFACMVKLYTLRKQAFTKPWSLWMWLTGVSIGCVCSVKWVGLFVTVVVGFYTILELFIMRFDSTMSKYDYMRHWMIRVLNLIVSPFLIYLFCFMIHFTLLYKSGTGDAATNTLFQINLEGNEIYSGPRTVVFGSHISIRSHGLSPNLLHSHVQTYPEGSNQNQVTGYGFADENNVWQVRFSRSSGVVWNENATSSDKLVPITDGTEIRLFHKEQEINLHSHEIPSHVSKGNFEVSGYGSESIGDSKDDWVVEIVRQLDSADATYPKEEYDVLHPLSTFFRLRHKDLGCYLTSTGFSYPAWGFQQAEIVCKYSWSKHDKSTWWNVEEHWNENLDINADYVPPKSKFWTDFVMINFAMASSNNALVEDGDKYDNLASKAWEWPTLHKGLRMCTWGVDNTRYYLMGSPFNTWLSTVSLVVFLIIMAKLLYQWRRQTVAITEDQFWSLTIQGVMPFTAWGTHYMPFVTMGRVTYVHHYLPALYFAVLVFGFVVDYLLRCSKKLIKYAVYLILFSGCISCYIYFSPMCQGMTGMWTNYLYLQWFKPWDIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T418</accession>
    <name>A0A8J2T418_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Multifunctional tryptophan biosynthesis protein</fullName>
            <ecNumber evidence="6">4.1.1.48</ecNumber>
            <ecNumber evidence="5">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BN860_16270g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two components I and two components II.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="6"/>
    <dbReference type="EC" id="4.1.3.27" evidence="5"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T418"/>
    <dbReference type="OrthoDB" id="524799at2759"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000136">
        <property type="entry name" value="Multifunctional tryptophan biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000031">
        <property type="entry name" value="Multifunctional tryptophan biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF4">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="15">
        <location>
            <begin position="8"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Indole-3-glycerol phosphate synthase" evidence="16">
        <location>
            <begin position="218"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CDF88695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="488" mass="53874" checksum="DB298A22A91483D0" modified="2022-05-25" version="1">MISRKVILIDNYDSFTWNLYQYLCEEGADVEVFRNDKITVEQVEALKPDILLISPGPGHPRTDSGISRDCIKHFTGKIPVFGVCMGQQCMFDVFGGEVAYAGEIVHGKTSPVHHDNKGLFKGVPQGVSVTRYHSLAGSEATLPDVLEISARLENGIIMGVRHKKYTVEGVQFHPESILTEEGHLMIRNMLRVTGGTWEENDCGENAAVAIKMGGGSILDRIYAQRRKDVDHQSNIAGFTNRDLQANFDLGLAPPLQDFYQKLTSCKGKAAVLAEIKRASPSKGDICPDAISAQQALRYAKSGASTISVLTEPHWFKGHLQDLVNVRRALDVEFHTKPTERPCVLRKEFVFCKYQILEARLAGADTVLLIVKMLPQEKLIELYQYATLEMGFEPLVEVSSKSEMDRALQLGAKVIGVNNRDLHSFAVDLNTTSNLVSAVPKNILLIALSGITTSKDAEKYKKEGVHGFLVGEALMRSANPSTFIRELSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T424</accession>
    <name>A0A8J2T424_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_19350g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_19350g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF88000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T424"/>
    <dbReference type="OrthoDB" id="48988at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19079">
        <property type="entry name" value="AKR_EcYajO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000004">
        <property type="entry name" value="Oxidoreductase, aldo/keto reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050523">
        <property type="entry name" value="AKR_Detox_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364:SF15">
        <property type="entry name" value="ARYL-ALCOHOL DEHYDROGENASE AAD16-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364">
        <property type="entry name" value="NADH-SPECIFIC METHYLGLYOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="19"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF88000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="338" mass="38396" checksum="ABEC59AA09C3D66F" modified="2022-05-25" version="1">MSLVGQVKFGTTGIKISPIIVGCMSYGSKLWAPWVLDDEEEVFEILKYCYDAGIRTFDTADVYSNGVSERLLGKFLQKYKIKRETVVILSKINFPVDESLGLSMNDVYHHNEATALDLANQRGLSRKHIISGAAQSVERLGTYIDVLQIHRFDPDTPIEETMRALNDVVESGLTRYIGASTMLATEFVEMQHVAETHGWFKFVSWQTQYNLLYREDEREIMPYSKKRGIALIPWSPNRRGFLTRPLGQQTERSKRDILRFNLSEADKEIIGRVEELSKRKGVSMAAISTAWVLSKGCNPIVGMSTIERVKDALSALSVELSEDETKYLEEAYLPKHLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T446</accession>
    <name>A0A8J2T446_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glucosidase II subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_12904g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism; N-glycan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T446"/>
    <dbReference type="OrthoDB" id="1334205at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017177">
        <property type="term" value="C:glucosidase II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090599">
        <property type="term" value="F:alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06603">
        <property type="entry name" value="GH31_GANC_GANAB_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF54">
        <property type="entry name" value="BCDNA.GH04962"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035285649" description="Glucosidase II subunit alpha" evidence="11">
        <location>
            <begin position="22"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 N-terminal" evidence="13">
        <location>
            <begin position="103"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="12">
        <location>
            <begin position="372"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="14">
        <location>
            <begin position="704"/>
            <end position="810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF88552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="947" mass="108766" checksum="9ACFEF8C4FCC2C85" modified="2022-05-25" version="1">MLLSWSLGLVFIWSQLETVCAFTQYLLKDCKQAGFCHRNRHYAEEIQKSERSYYSIDADNVYYDENEKVVRATILKTIFQGDNRNNEQKVELPFTLGLLQDGATVRFTIDENRPHKNYLPSSLNQRRYNEASKWAFEDWQNLPKYEATMRKGFLNRNKVIVEGDCDYICKLKVELYTKSFLIKIYYKEALIMVVNERSLLNYEHLRAEQENESNLLLEESDFNMFHDNFKYSKDDTMPFGPESVALDFTFSEFEHLYGLPEHPGSFRLQDTSNGEPYRLYSTDVFRYDIGTRMPMYGNIPFVIGNNQRAAAGIFWVNAADTWVDFKYTRDAKKTPKSQSHWMSETGIIDVVLFFGDTPSEITEKYTAITGRPALPKQAAIGYHQCRWNYNDELDVLTVDSSMDRDHFPYDNIWLDLEYTDNKKFFTWKPNAFPNPDRLLKKLAKLGRDLTVLIDPHLKAGYNVSNAVVADHAAIKNHEGDTFYGTCWPGKSVWIDTMGDIGRKLWAKFYEAFLNGAKNLDIWNDMNEPSIFDGPETTAPKDLIHAGGFEQRALHNVYPLTVHQATYDGMINIFNESSRPFIIARGHFSGSQRTAGTWTGDNVANWDYLKISIPMILSSNVAGMPFTGADIAGFFDNPEDELVVRWYQAGLWYPFFRAHAHIDSLRREPYLFEEPIKSHIRNAIQLRYNLLPTFYTAFYEASIDGTPIMKPMVFEKPEFLDLALVEDQFYLGNSGLVVKPIVEKDAVSTTMLVAEGLYYDYWDLRPYRGGTIIPDDEFVENLPALTFNVKPIRVNAPLDKIPILLEGGHIISKREKYRRSSKLSRNDAYTLVVAPDNYGNAEGKLYADDGETLAHKHGEYLLSTFTMQNNELLTGHPTHNTLNKASLGNTLIDKIIIASSTMKSPPKDTVTVKKGLSRYAVKAVRTGPYETTIFNPLVHADETWEIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T462</accession>
    <name>A0A8J2T462_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_11188g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T462"/>
    <dbReference type="OrthoDB" id="347657at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000196">
        <property type="term" value="P:cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010606">
        <property type="term" value="P:positive regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060211">
        <property type="term" value="P:regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05581">
        <property type="entry name" value="STKc_PDK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000191">
        <property type="entry name" value="3-phosphoinositide-dependent protein kinase 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000534">
        <property type="entry name" value="Serine/threonine-protein kinase PKH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039046">
        <property type="entry name" value="PDPK1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF163">
        <property type="entry name" value="3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="177"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="33"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="476"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="549"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="762"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="917"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="499"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="549"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="588"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="606"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="784"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="917"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="955"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="990"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF87644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1091" mass="120385" checksum="3CA063300E67CFF4" modified="2022-05-25" version="1">MPFNETSRPDMRPLLPGDEEQINSQFTKKHAALQSSALNRRSVGPVGVPLRSTSSRNVPEMLDSDVPMHRALTNADNFISMYNADGSSEEMTSNSYADSNNHSGELGPGEDDQLSLGSDDNVSHPIEMAHGMQLDPGLKKHREEWADKGAAKIVRDVTNPKTGQTTREVIRKGIKDFKFGDTLGDGSYSTVMVATSKENGKKYAVKILSKEYLIRQKKVKYVNIEKNALQRLNNSRGIIKLYFTFQDESSLYFLLEYAPNGDFLSVMKKFGSLSEECACYYSAQIIDAINFLHTKGIIHRDIKPENILLDKEMKVKLTDFGTAKILEPKQQSHGSGPSYDLLTRSKSFVGTAEYVSPELLNDNWVDYRCDIWAFGCILFQMIAGKPPFKATNEYLTFQKVMKVQYAFTAGFPLVIRDLVKRTLIKQPDQRLTIPQIERHHFFKDKNFKDGSVWEEPAPEIQPYRMTAKSMQPVPALNTNHHPLKRPWNVRPNSSKISVNTNTDNASSTTSGSNATSPLAATPGNHSQSKPSSPVIVQKRVMDEKTAQILDNAKRAVDGRKGNSHRKYSASASALAPTGKKNYGPPETPSSVSSASLAPSIPHVRSKSSATTKNTGNYASPMISPSNNSSSNTVDSARRSSESPLLSPVTAAQPWSRLGGDESSSIDPHDTAWGYHLTNPDEHVLEAAELNFAIVDNVSLERRVHKSRGSLTESQRHGPSRATLLSQVARGGGGVTGLRSDSDAPLLEPDFYKKCHISKDDLAPEYKIPGGDPSLSPPEERERSPNLSEDNPNSPISGKFKRFFYHGGKQESGNDVIDIGNYHRRMLVVTSFGRALVFARKKRNISNSSTNYDLCYDINICQSGIKLREVNVPSITSKSGNFVIQTPFDSFLFRTTKTSSENWLLTLHESIRVNHERLVSNSRKEDGRHKSSSVDAMKAAKLATPTIAKERETHYTPKSSKPLSPQLNSHPKTPKTPRSSFYSLGSGGSSGSLTGSVPSGRNGKNERMFDSFVNSKEKHGKKRASPVPMSSKLVNGLPFHNYSAPVGLGIPSHSQSSINSVTPASAKTVKKTINPGNSRLLARSEQTFRRKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T470</accession>
    <name>A0A8J2T470_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Arginine biosynthesis bifunctional protein ArgJ, mitochondrial</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="10">Glutamate N-acetyltransferase</fullName>
                <shortName evidence="10">GAT</shortName>
                <ecNumber evidence="10">2.3.1.35</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">Ornithine acetyltransferase</fullName>
                <shortName evidence="10">OATase</shortName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="10">Ornithine transacetylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="10">Amino-acid acetyltransferase</fullName>
                <ecNumber evidence="10">2.3.1.1</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">N-acetylglutamate synthase</fullName>
                <shortName evidence="10">AGS</shortName>
            </alternativeName>
        </domain>
        <component>
            <recommendedName>
                <fullName evidence="10">Arginine biosynthesis bifunctional protein ArgJ alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="10">Arginine biosynthesis bifunctional protein ArgJ beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_18514g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-glutamate + acetyl-CoA = N-acetyl-L-glutamate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(2)-acetyl-L-ornithine + L-glutamate = N-acetyl-L-glutamate + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:15349"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.3.1.35"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="10">The alpha and beta chains are autoproteolytically processed from a single precursor protein within the mitochondrion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ArgJ family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.35" evidence="10"/>
    <dbReference type="EC" id="2.3.1.1" evidence="10"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T470"/>
    <dbReference type="OrthoDB" id="2017946at2759"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00106"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004358">
        <property type="term" value="F:L-glutamate N-acetyltransferase activity, acting on acetyl-L-ornithine as donor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006592">
        <property type="term" value="P:ornithine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02152">
        <property type="entry name" value="OAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.70.12:FF:000001">
        <property type="entry name" value="Arginine biosynthesis bifunctional protein ArgJ, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.340:FF:000002">
        <property type="entry name" value="Arginine biosynthesis bifunctional protein ArgJ, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2330.10:FF:000001">
        <property type="entry name" value="Arginine biosynthesis bifunctional protein ArgJ, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2330.10">
        <property type="entry name" value="arginine biosynthesis bifunctional protein suprefamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.340">
        <property type="entry name" value="ArgJ beta chain, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.70.12">
        <property type="entry name" value="L-amino peptidase D-ALA esterase/amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01106">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002813">
        <property type="entry name" value="Arg_biosynth_ArgJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042195">
        <property type="entry name" value="ArgJ_beta_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00120">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003802">
        <property type="entry name" value="PRK05388.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100">
        <property type="entry name" value="ARGININE BIOSYNTHESIS BIFUNCTIONAL PROTEIN ARGJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100:SF0">
        <property type="entry name" value="ARGININE BIOSYNTHESIS BIFUNCTIONAL PROTEIN ARGJ, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01960">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="DmpA/ArgJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 10" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 10" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_5035349229" description="Arginine biosynthesis bifunctional protein ArgJ alpha chain" evidence="10">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035349228" description="Arginine biosynthesis bifunctional protein ArgJ beta chain" evidence="10">
        <location>
            <begin position="210"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="436"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="10">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="10">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="10">
        <location>
            <begin position="209"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF87964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="436" mass="47190" checksum="C0A8A2F53A267DEA" modified="2022-05-25" version="1">MHLSAPLKQSAKYAKHVPKSGVFPKGFEVASWASGVKKNGNLDLGILVNTNKSRASSAAAVFTTNKFKAAPVMLSQKLLNATNGKGINAIVVNSGCANSVTGKVGMQDAQQMVQYVNENLGCPNSTLVMSTGVIGQRLQMEKIRKGIQSIFDKHAFGNDFNSWLNIAKSICTTDTFPKLVTSTFELDNGTKYTMTGMAKGAGMICPNMATLLGFIVTDLPIESNALFKMLRFATDRSFNCISVDGDMSTNDTINMIANGAVETAEINESSPEFEQVRDQVTEFAQQLAQLVVRDGEGATKFVTVNVKNSLTFADAKIIAETISNSMLVKTALYGKDANWGRILCAIGYAKLDNLQSLQADKLNVSFIATDSSEPKELKLLSNGVPQLDIDEERASQILALPDLEVLVDLGTGKEEAQFWTCDLSHEYVTINGDYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4A2</accession>
    <name>A0A8J2T4A2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Biogenesis of lysosome-related organelles complex 1 subunit KXD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">KxDL homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_16710g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the biogenesis of lysosome-related organelles complex-1 (BLOC-1) involved in endosomal cargo sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the KXD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4A2"/>
    <dbReference type="OrthoDB" id="4089816at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051390">
        <property type="entry name" value="BLOC-1_subunit_KXD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019371">
        <property type="entry name" value="KxDL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37787">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT KXD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37787:SF1">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT KXD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10241">
        <property type="entry name" value="KxDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="KxDL" evidence="9">
        <location>
            <begin position="89"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF87886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="189" mass="21319" checksum="6224EC2D77DDA060" modified="2022-05-25" version="1">MTDTSNGSQHDAASSRSPSVNSQSYAIPMPAEIEEQLTEDSSSLNDSLSAQSNEEGFLERAIPESGRAFSHDSQEVGPMLDVSKYIFESLVQSIDSADFSEAISLQTKTSAIINSKSMELKQIIDDTKVRFHQLKERFERGAITSRKVRHNLQYSKEKIDKLNALLRTDYPIEFNQAREKVLERQLDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4H6</accession>
    <name>A0A8J2T4H6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BN860_12794g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_12794g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxoprolinase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3643at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049517">
        <property type="entry name" value="ACX-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF2">
        <property type="entry name" value="5-OXOPROLINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19278">
        <property type="entry name" value="Hydant_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="5">
        <location>
            <begin position="7"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="3">
        <location>
            <begin position="242"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Acetophenone carboxylase-like C-terminal" evidence="6">
        <location>
            <begin position="548"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase B/oxoprolinase" evidence="4">
        <location>
            <begin position="744"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="543"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF87715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1282" mass="140061" checksum="B926B4C63B800234" modified="2022-05-25" version="1">MATSGIRIAIDRGGTFTDCVGNPGTGRQEDDVVIKLLSVDPKNYADAPLEAIRRLLGMFSGKEIARGVPLDMSGVSSIRMGTTLATNCALERNGEPCAFVTTKGFRDVIVIGNQSRPDIFDLNIKKPKPLYETVIEVDERVTLEDFSEDPLNHRSVPCAEQNTVLGTTGEVVRIVKALEVEEVRRSLRRVYESGIRSVAVALLHSYTYPGHEQLVGRIAREIGFKHVSLSCEVSPMIKHLPRAHSAVADAYLTPVIKKYLESMQSGLVNVENTSIQFMQSDGGLVEGHRFSGLKSILSGPAGGVVGYSSTCYDAENGISLIGFDMGGTSTDVSRFGDGKLEHVFETTTAGIVIQSPQLNINTVAAGGSSRLFWENGLFRVGPESATADPGPAAYRKGGPLTITDANLVLGRLVPEFFPRIFGPNEDESLDLEATRKKFDELTGIINEDMQASMRPEEVALGFLKVANESMARPIRAITENKGHVVSKHRLVTFGGAGGQHAVAVAESLGIREVVAHRYSSILSAYGIFLADVVEENQEPCFLLLNEEENVRQVELKLERLVEKSEESLKAQGFEKDHIFFEKYLNLRYEGTETSLMILEESNQWDFEQWFAAAHKREFGFAFSDKNIIVDDIRVRAVGKSHVRKEESVDDQLKRYPSREVSVSREASFFKDVFFDNVWVRTPVYRIDDMRYGSVVKGPAILADGTQTNIIPANSVAHVLKSHIFIEILHKSGNGISGEPEFHVDPIMLSIFSHRFMDIAEQMGMQLQKTSVSTNVKERLDFSCALFDPEGNLVANAPHLPVHLGSMSTCIAAQARLWKGRLKPGDVLMSNHPDIGGTHLPDITVISPAFSESSGKLIFYVASRAHHADIGGILPGSVPPNSKELYEEGAAIYSELIVREGVLQEAAIRRLLLEEPAKYPRCSGSRRISDNISDINAQIAANNKGIQLISKMISESGLDVIVKYMTAIQENAAQTIKKMLQKLTEHFGETHFSGEDRMDDGTVIKLAVSLDIEKEEYVFDFEGTSPQVYGNLNAPEAITYSAILYCLRCLVGEDIPLNQGCLKPIIVKIPKGSILSPINGIAVVGGNVLTSQRVTDVVLKTFHVMADSQGDCNNFTFGTGGRDPETGVVSKGFGYYETICGGHGAGADSWRGAGWNGTSAVHTNMTNTRMTDSEVFERRYPVLLREFSVRTGSGGKGRYTGGDGAVRDIEFRKPVEASVLSERRVIAPHGLNGGNDGQRGLNLWIRDQGKNIINIGGKNSVKVTPGDRILLMTPGGGGCGKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4K7</accession>
    <name>A0A8J2T4K7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_17326g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_17326g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4K7"/>
    <dbReference type="OrthoDB" id="4048430at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055425">
        <property type="entry name" value="IgD1_Trs65"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055426">
        <property type="entry name" value="IgD2_Trs65"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024662">
        <property type="entry name" value="Trs65"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28159">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX II-SPECIFIC SUBUNIT 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28159:SF1">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX II-SPECIFIC SUBUNIT 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23645">
        <property type="entry name" value="IgD1_Trs65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23646">
        <property type="entry name" value="IgD2_Trs65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trafficking protein particle complex II-specific subunit 65 IgD1" evidence="2">
        <location>
            <begin position="2"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Trafficking protein particle complex II-specific subunit 65 IgD2" evidence="3">
        <location>
            <begin position="176"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF87913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="484" mass="54834" checksum="972688CE677A6D6F" modified="2022-05-25" version="1">MECYIPLGKLADNAAIAEVKQSHDSRRFVVFDEDLSIYLRAGDTTRLHRFTVWINDAKVLQSQGVASFNKVTGLDACWILKPGTTDSLFRSSVVMNNGYNNQIKFTVEYVEDVPISDDQKSSSEDGDNDILPSFEPVLPGQPPKQNANSIHQSGLSIMNVPPEQLKSITLQYPIYSLLNMRLRNSMLKNRHCIISSLDFQTSKASIQFSERYLPSSRPDGIRPELRLDFQELRYELVDRTARCPLDPITPFPVPFQAVAHDSYSISYQLPLVPDHNLSPHRVKITLKYVMPLGNHRLPITTTWETDVTLKKPAITNTPQPSSSLPTSRMYTLGSRINLLGSTTSFTNNKLNNVKFKFLDKNVNAYKGAQFTMTLQIVNSSTQPLDLVIYYNNRNPPPHPQISVPLSLDKQYQMYKRYRRTTEGVILLSNDYKVPLVGPHETYFVRLNFVGIISGYYSTLPGLKIVDLQRNELIEVGLGASIYIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4L6</accession>
    <name>A0A8J2T4L6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">BN860_08548g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_08548g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4L6"/>
    <dbReference type="OrthoDB" id="28413at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF88365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="640" mass="73457" checksum="E1660EE6332366C4" modified="2022-05-25" version="1">MDATSRVQQLLDQSESLSLEHSRECLKTILAASHQPKQFDFSRFLLGCHDKVLCQEAILNAKFWELFEVTMTDAPPDTLAICLTRVCTLSLRESGVDMQAITSLCRFLSTSDMILPILSANLSTQRTSMRVNFDILESVDGALRCLVCLCERDPMNAAVIVPSSFFLFQDCGFWNILISLLPMEELKSEILEVILPLRNRIFKFMASLSLKEFPTPLETKMMESITDTLSFDGNLKSKFEEVNQGYGDLHSIKLIEAFHLVIFLKNANLSFKKTLSEELLFGSNPFPLYKAFFSILDQLHQFLNSQAKDTSTTTYYAAFLLDKDAFIYTIMDKILKMWVVSQGESKNDIHSLLETIPIMLDQIDKAIKNFSDLSPQGAVSLALETIEGINYEKARQLQLESIKRKHQEKWSNATSDFEKLLSEQVHDYVRHQRLLQLQKGTWAFSENPLDRNVPCPKLYFMVLSANQVNLLVREFPTKMEVTPFIEENGIFSSSEKDQAGFEKTTAIPLHTIVDFETAEILLENKAPDNPRLVNVIQKDFYTGVNFLDKYRKTVLKVFFDNKDDVYVWLDGLKLVSPFKIRRGISDSTKEQMNTLIDIRRNAQMLNLEISDDGESTENSDEEEYYNVDTLKELNLKFYYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4M2</accession>
    <name>A0A8J2T4M2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Bis(5'-adenosyl)-triphosphatase</fullName>
            <ecNumber evidence="3 13">3.6.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_17700g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Cleaves A-5'-PPP-5'A to yield AMP and ADP. Can cleave all dinucleoside polyphosphates, provided the phosphate chain contains at least 3 phosphates and that 1 of the 2 bases composing the nucleotide is a purine. Is most effective on dinucleoside triphosphates. Negatively regulates intracellular dinucleoside polyphosphate levels, which elevate following heat shock.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 13">
            <text>P(1),P(3)-bis(5'-adenosyl) triphosphate + H2O = AMP + ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13893"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.1.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.29" evidence="3 13"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4M2"/>
    <dbReference type="OrthoDB" id="680339at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047710">
        <property type="term" value="F:bis(5'-adenosyl)-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01275">
        <property type="entry name" value="FHIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000011">
        <property type="entry name" value="Fragile histidine triad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051884">
        <property type="entry name" value="Bis(5'-adenosyl)-TPase_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039383">
        <property type="entry name" value="FHIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46243">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46243:SF1">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="14">
        <location>
            <begin position="5"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="12">
        <location>
            <begin position="98"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="9">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="93"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for induction of apoptosis" evidence="11">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF87930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="190" mass="22575" checksum="67E24A70F7693233" modified="2022-05-25" version="1">MNMTNAVYFSKFLVTKQVFFQTNHSYALVNLKPLVPGHILIVPLRTQVHNLCDLTSEETLDYFDTLQVIHQFIKWQFKADSLNIAIQDGPEAGQTVPHLHTHVIPRYRLNNVGDRFYEDLDKWRFNDWEQRRQDYLSMGAREGRKAFAKPDDQRYARTQEDMEDEARYLANQLNKFFLENPYLKRECLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4N5</accession>
    <name>A0A8J2T4N5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BN860_18360g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:35000"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 16">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4N5"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF14">
        <property type="entry name" value="ADP_ATP TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00927">
        <property type="entry name" value="ADPTRNSLCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="11 14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="3"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="108"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="206"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CDF87958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="299" mass="32814" checksum="0D993D530FAC427D" modified="2022-05-25" version="1">MQEDFLANFLMGGVSAAVAKTCAAPIERVKLLIQNQGEMIKQGTLEQRYVGITECFSRTIKSEGVLSLWRGNTANVLRYFPTQALNFAFKDKIKAAFNVKRETEGYTKWFAANLASGGAAGGLSLAFVYSLDYARTRLAADASRNGAERKFKGLRDVYRQTLATDGVMGLYRGFGPSVVGIIVYRGLYFGLYDSLKPILLTGSLERSFAASFLLGWVITTAASTASYPLDTVRRRMMMTSGQQIKYSGAWDCLTSIYKQEGPLTLFKGCGANIVRSIAAAGVISLYDQLQLLMFGKTFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4Q3</accession>
    <name>A0A8J2T4Q3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Actin-related protein 2/3 complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_19064g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Cytoskeleton</location>
            <location evidence="6">Actin patch</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ARPC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4Q3"/>
    <dbReference type="OrthoDB" id="336240at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1460.20:FF:000001">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034666">
        <property type="entry name" value="ARPC2/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008384">
        <property type="entry name" value="ARPC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22629:SF0">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22629">
        <property type="entry name" value="ARP2/3 COMPLEX 20 KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05856">
        <property type="entry name" value="ARPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039100">
        <property type="entry name" value="ARPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69645">
        <property type="entry name" value="Arp2/3 complex subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR039100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF87988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="171" mass="19950" checksum="CD2CCD85B4B196A4" modified="2022-05-25" version="1">MSQSLRPYLTAVRYSLEAALTLSNFSSQEVERHNRPEVEVPNTSAELLLQPIHISRNENEQVLIESSVNSVRVSIKVKQADEIEQILVHKFTRFLEQRAESFYILRRMPMPGYSLSFLITNKHTETMKTGKLVDFIIEFMEDVDKEISEMKLFLNARARFVAEAYLDQFVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4V0</accession>
    <name>A0A8J2T4V0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Ribosomal RNA-processing protein 8</fullName>
            <ecNumber evidence="9">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_12112g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(1) position of adenine in helix 25.1 in 25S rRNA. Required both for ribosomal 40S and 60S subunits biogenesis. Required for efficient pre-rRNA cleavage at site A2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the methyltransferase superfamily. RRP8 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="9"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4V0"/>
    <dbReference type="OrthoDB" id="10258825at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016433">
        <property type="term" value="F:rRNA (adenine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2150:FF:000001">
        <property type="entry name" value="Ribosomal RNA-processing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000320">
        <property type="entry name" value="Ribosomal RNA-processing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2150">
        <property type="entry name" value="Ribosomal RNA-processing protein 8, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007823">
        <property type="entry name" value="RRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042036">
        <property type="entry name" value="RRP8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787:SF0">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05148">
        <property type="entry name" value="Methyltransf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="24"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF88517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="376" mass="43822" checksum="0F3906C535509893" modified="2022-05-25" version="1">MVLFNVDGWDLKTDNVIYYDASSERAAQKKKEQKEKKKEQKKQQNLKKSRHEEPTNKLANASKRKMEEPTSPLKENKVKFAKLELKAAPRTEKTLTPLQQKMMAKLTGSRFRWINEQLYTISSEDALRLIQEQPQLFDEYHDGFKSQVASWPENPVDVFVEQIRQRSKKPINAPGGLPGLPTKQIVIADMGCGEAQLALDINKFFSQYNKKSKKYQQKSHVVHSFDLKKANDRITVADIKHVPLPDSSCSIVVFCLALMGTNFLDFIKEAYRILAPRGELWIAEIKSRFADGKGDEFVEALKLLGFFHKKTDNENKMFTRFEFFKPPQDIIDERRAKLERRHKFIETENEKEALEKKRLQIAEGKWLLKPCIYKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4X8</accession>
    <name>A0A8J2T4X8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Guanine nucleotide-binding protein alpha-1 subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">GP1-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_18888g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4X8"/>
    <dbReference type="OrthoDB" id="5817230at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000012">
        <property type="entry name" value="G protein alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G protein alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000563">
        <property type="entry name" value="Guanine nucleotide-binding protein alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Fungi_Gprotein_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF302">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-5 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01241">
        <property type="entry name" value="GPROTEINAFNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="2" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="52"/>
            <end position="57"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="288"/>
            <end position="294"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="313"/>
            <end position="317"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="382"/>
            <end position="385"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00078004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF87980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="466" mass="53630" checksum="A9F48D976DC65CBA" modified="2022-05-25" version="1">MGCVASSPGSVENENDPFLQSQRANDAIEQSLQMGKQNDKNEIKLLLLGAGESGKSTVLKQLKLLHKGGFTHQERLQYSQVIWADAIQSMKILVIQARKLGIPLDCDDPLNNRELFECKRILLKAKALDHIDAGVAGGSDFLNDYVLKYSERHETKRRDQSTGQARAFDEDEQSWLLRDKSEDRAMSDETVVVEDKLDLQQIFRDLDEDSGNDQMFLKEQQLQQRQQQQLTNLDIANAIEQLWKKDKGIKQCFARSNEFQLEGSAPYYFDNILKFADRDYICSDEDILKGRVKTTGITESDFTIGSSKFKVLDAGGQRSERKKWIHCFEGITAVLFVLAMSEYDQMLFEDERVNRMQESIMLFDTLLNSKWFRNTPFILFLNKMDIFEEKVKRMPIRKFFPNYQGRVGDAEAGVKYFENMFLSLNRSNKPIYVKRTCATDTQTMKFVLTAVTDLITQQNLKKSGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T509</accession>
    <name>A0A8J2T509_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_14224g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T509"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF137">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE HRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="176"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="419"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="419"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="448"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="488"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="521"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF88608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="637" mass="72570" checksum="E2EF29C27F8B8584" modified="2022-05-25" version="1">MPNLLSRRPFHHEHHHHHEHSPNSKHSRSFLSFAHKRQSNDSIKSDKSTDSLHSAYSPHGQGQGFSLQEFMGSSSPKSSHSMVELKRFLRPKINRKSSGSSGHHHRGGVRPSASTVELPQNLLQQSQQYAKSNSQEAADHHTHTQSAIPPSSDSTLSLSNKVNIYHDDSILAQKYGKLGRVLGSGAGGSVKVLWRPSDNATFAVKEFRPRKQNESVKDYAKKCTAEFCIGSTLRHCNIIETMDIFSDTNQNRYFEVMEYAPVDFFAVVMTGKMSRGEINCCLKQIMEGVKYLHSLGLAHRDLKLDNCVMTLDGILKLIDFGSAVVFRYPMEVGVTPAHGIVGSDPYLAPEVISHSKCYEPQRADIWSIGIIYCCMMLKRFPWKVPKESDENFRLYCLPDDEPHDYVASAKHHEELLNERRERHERQQRGEVEPEDECENHHHHHHHIHGEEHHHHQEAKKAEKEQQQEPEKTEKEQQPEGGKCNQESQDPKHEQMQEAEGTKKEQGSDDTKGAQQHDEQHSQQVSQQADQQSQIDAKSVASHPKPPASSQNPPHHRRILHGPYRLLRLLPHAARPIMSRILDVDPTKRATMKDIYDDDWFAAIQFCTVNDKKEVVRAPNHHHTLVREDANGSETFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T511</accession>
    <name>A0A8J2T511_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ZYBA0S03-09846g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_09846g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T511"/>
    <dbReference type="OrthoDB" id="240216at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004092">
        <property type="term" value="F:carnitine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000019">
        <property type="entry name" value="Carnitine acetyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000003">
        <property type="entry name" value="Carnitine acetyl transferase FacC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF29">
        <property type="entry name" value="MITOCHONDRIAL CARNITINE O-ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="6">
        <location>
            <begin position="7"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF89129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="678" mass="77618" checksum="5EE87022EA1A08E4" modified="2022-05-25" version="1">MTTLRRLPIPSLRDTLDKYLSRVEPLQDERQNQKTRRCVLSTENLQRMQKLHEHLVEADKELAKENPQSSYIEQFWYDAYLLYDASVVLNVNPYFLLQDDRTITQTAGCYGKYTCQVRRTAKLVFSILKFVREIRHGTLKPDIVRGKVALSMDQYSRLFGGSRIPPAPGKDSCHLQVDPTSHHVIVMYKSQFYWFDVLDVDNMPIFSTPEQIEWNLYSIIMDAEQSEEPTGPPFGVFTTESRRVWSNVRDYIFHDADSTNWKNLKIIDSALFVVCLDDVSLTQDNELVKSMLCGTSQIVLDPKNLARPLDAQHGTCLNRWYDKLQLIVTQNGKAGINFEHTGVDGHTVLRLAADIYTDSILGFARGVTKNVPDVFSESNTTSSHAGNLNLITIPRKLEWKVDPFLLSSLHFAETRISDSVSQHDFVTLDFTKYGATHIKAAFQCSPDAFVQQMYQLAYYALYGKFETTYEPAMTKAFQNGRTEAIRSVTHESKKFVKSMFDRYAFDDHRVRFLREACDKHVSIIKECSMGLGQDRHLYALYCIWNETFKDSMPLPPLFNDKSWSLLNTNVLSTSNCGNPCLKSFGFGPVTANGFGIGYIIRDDSITFAVSSRHRQTQRFVSLIEHSLLEIDHVFSRKSKAEPIVRALQQDKTAEQQRRSDNLSYLLSGYDYFDVSVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T516</accession>
    <name>A0A8J2T516_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_06194g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_06194g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat WDR6 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T516"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051973">
        <property type="entry name" value="tRNA_Anticodon_Mtase-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14344">
        <property type="entry name" value="WD REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14344:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="175"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="217"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF88273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="982" mass="110923" checksum="E68718F953301B4D" modified="2022-05-25" version="1">MEVLSHYGPALCVKFFNGYVYTGYGPFIQVYDYHTGDLKNQCRIFETNKVHGVTFSNSGMALCYGSKSTSLMPLGELSKRSSLVEYERRIGEWIISAEFNYDNNGFFLLTCYNRVLSFDLLGNLIRKSAVYGEKSLLYSGSIKVVSSCKTYVNAGTVMGGIIIWDLFSESIVANLLGHSGSIFYVTMNNDGTLVTSCSDDRSVRLWDLKSQTELSVGWGHTARIWNLLFFNNDSRIISVSEDCTCRIWAVEDNKLLQMAIYEVHLSKNVWGVDVNEQDQIAITSGNDGRLKLIELQPISDSQQVFTLQEISTQSCMIFGKEEIIKGFHWFAFGLIAITSMGKVLKFDDNEGRWEFVLQSDLFTSYSVTNGITNANIVIFSNNKCDLLILKLDPCNGRIIDRQEMHINELSKTQNCMVCRHNSIQDCFLITLESPNPHDPFLCLKFDINTLQLENKYSFIKPVNFSSSCLEVYDSFLLVGSRFSTLAIFNFNNVEQEPYVFRRLTPGDTTTSIHFVEYSQNKHVFAVTNRDGYYTFIGANLDSVEFNGSLKCEVIHDNKISRGFLEGAIFEDSGDLITFGFKSELFYIYNESQCYEIASLVCGGAHRQWKFSRTPDGFILIYIRASSIYFAKIGVSALPRTLRCGTHGREIRDISILKDRKYKNGYLFCTASEDTTIKLCHIEEATGNISTFWTLRKHVSGLQRCKIINDKFMISSSAREELFLWQITTDCNSKPYVTLRQSLPIFCDNPDLRIMDFAVKFVETDAQGFLLATVYSDSAIKLWRYNHQESKFHLLLDGRYESCCLLNVSLFSLDDQLYIMITATNGCLVVYNITDLSNGGDLAPFPDVDCSIRTHQSGIHALDIAVDNTGHSVTAYTGGDDNGLGICYFHLDKFTNRLTPKIKDFQRNAASSTITSCKLLEGREQLLTTSVDQVLRRWSVAGDKLVLEEQRYTSIADTGSLDVVENLLVVGGVGLSVMKNEHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T526</accession>
    <name>A0A8J2T526_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">TATA-binding protein-associated factor mot1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Modifier of transcription 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_06568g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10252227at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17999">
        <property type="entry name" value="DEXHc_Mot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000009">
        <property type="entry name" value="B-TFIID TATA-box-binding protein-associated factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000428">
        <property type="entry name" value="TATA-binding protein-associated factor 172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044972">
        <property type="entry name" value="Mot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044078">
        <property type="entry name" value="Mot1_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022707">
        <property type="entry name" value="Mot1_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36498">
        <property type="entry name" value="TATA-BINDING PROTEIN-ASSOCIATED FACTOR 172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36498:SF1">
        <property type="entry name" value="TATA-BINDING PROTEIN-ASSOCIATED FACTOR 172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12054">
        <property type="entry name" value="DUF3535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="1300"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="1652"/>
            <end position="1803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="154"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1821"/>
            <end position="1851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="175"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="237"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00081329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF88288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1882" mass="212299" checksum="A45948EC012ADA5E" modified="2022-05-25" version="1">MTSQVSRLDRQVILLETGSTQFVRNMAADQMGDLAKEHPEDILSLLSRVYPFLLVKRWETRVTAARAVGGIVCHAPLWDPNEGEREGDKKFEEQVNDETNAKVKLEHEMQIKLEEATQNDGLLEDSRPMLSLADWNLHELLKSKKVLLASNMNDYNDGNARKQSKVDSTGTESYEELKEINNGVKKEENNEGKVMNSKKASSPSVATSKKSARMLAMAKRKKKMQAKGSSSKPVDLSESSVSKSLMSQHLAKGKDGSMSPGSTPTSLSNPKLEITDQVDDSKIMVESMMAPILEKHEKVAGLVWQFQGVFELLLDNLINDSWEVRHGAALGLRELLKKHAFSVNRVAGKSRAENNARNRRALEDVSTRLLTVFAIDRFGDFVYDTVVAPVRESVAQTLAALLIHLDDDLCIKIFAALEQLVLQDPRVVMMPNKIWEATHGGLLGVRYFVSIKTDFLLSHNLLNNVVNIVLYGLNQGDDDVQSVAAAILSPITDQFVKLDPQAIELVLTTIWSSLTHLEDDLSSSVGSVMDLLAKLCKHQEVLDILKQKALHQPSEWSFKSLVPKLYPFLRHSISSVRKAVLNLLHAFLSIKDDSTKSWLNGKVFRLVFQNIILEQNREILDLSFNVYSLLLRDYRFKHTEKTLDHVFSKHLQPILHLLNTPIGENGKNYSMESQYILKPSQHYQLHADKKRTISESNFDSSDIPPPKNSERVNIDEPMIAGDITLLGAEVIQNTRIMGAKAFGLTLSMFQETTLQSFFANVLARCFELSYATPRMIAGIVLSSFCSDWESNHPNEAIPAFVREIFGPIIHAQLLNPEILPTFRELVPSLKALRTQCQSLMRTFVDVGMLPQHKFPNLPIIVQGEAEAGPEAFNIDLAEKVHDEYYDKMFRSMSNSYKLLAKKPLEDAKYRVFLAIESAKESKRSRNSSILSNYAAAFLRFEGLPKKLNPIIRAFMDSIKEEKNEMLQQLAGDSIIYLVEKLLAESKDNVANKICKNLCGFLCVDTTEVPEFAAHSQLKDSLLTLIKENSPPPSPDDVHLRKIAEDAQIKRKGGLYTLGQLFKIFGSNTLKRVKQLRVSLYEPLDNLSEWIGQDNLSNVTGQFVVDALGILRAIYPYMSEDLRANEVWPRFPVLLKLLNCEFSVIRYSTARTFADLAKLSPIPVMTFIIREVLPLLNSAGSVIDRQGAIELVFRLSISMGTDILPYVIFLIVPLLGRMSDSNNDVRSLATTTFALIIKLVPLEAGIADPEGLPEDLMAGRERERDFIQQMMDSSKAKPFKLPIAIKATLRKYQQEGVNWLAFLNKYHLHGILCDDMGLGKTLQTICIIASDQYLRRENYEETHSVETRPLPTLIVCPPSLTGHWEAEFEQYSPFLKTLVYAGGPSTRGPLRDKLPEADVIITSYDVARNDLSVLAQFDYNYCVLDEGHIIKNAQSKLAKAVKQFRSNHRLILTGTPIQNNVVELWSLFDFLMPGFLGTEKMFQERFAKPIAASRNSKTSSKEQEAGALALEALHKQVLPFMLRRLKEDVLSDLPPKIIQDYYCELSDLQKQLYADFSRKQKTVVKKDIENTTDVDSKQHIFQALQYMRKLCNHPALVLSPEHPQLKQVESYLKQTHMDLHDISNAPKLLALRNLLFECGIGEEDMGRKSPPNQLPSTANVISQHRALIFCQLKDMLDMVENDLFKRYMPSVTYMRLDGSVEARDRQKVVRKFNEDPSIDCLLLTTKVGGLGLNLTGADTVIFVEHDWNPMNDLQAMDRAHRLGQKKVVNVYRIVTKGTLEEKIMGLQKFKMNIASTVVNQQNNGLASMNTNQLLDLFDTDNVPSQEKEEKSSQPTNGEGLEEVADETGLSGKAKEALGELKELWDPSQYEEEYNLDNFIRTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T587</accession>
    <name>A0A8J2T587_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Coatomer subunit beta'</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN860_05292g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasmic vesicle</location>
            <location evidence="1 13">COPI-coated vesicle membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Golgi apparatus membrane</location>
            <topology evidence="13">Peripheral membrane protein</topology>
            <orientation evidence="13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="13">The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the WD repeat COPB2 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T587"/>
    <dbReference type="OrthoDB" id="10261470at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22947">
        <property type="entry name" value="Coatomer_WDAD_beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000016">
        <property type="entry name" value="Coatomer alpha subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000001">
        <property type="entry name" value="Coatomer subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016453">
        <property type="entry name" value="COPB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF2">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005567">
        <property type="entry name" value="Coatomer_beta'_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 13" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <keyword evidence="5 14" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="9"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="93"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="136"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="196"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="224"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B second beta-propeller" evidence="16">
        <location>
            <begin position="326"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="17">
        <location>
            <begin position="607"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="822"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="837"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="864"/>
            <end position="873"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CDF88233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="883" mass="99024" checksum="DA8C5961A5659718" modified="2022-05-25" version="1">MKLDIKKTFSNRSDRVKGIDFHPTEPWVLTTLYSGRVEVWNYETQSEVRSIQATDSPIRSGKFIARKNWIVVGCDDYRIRVFNYNTGEKVTDFEAHPDYIRSIAVHPTKPYLLSGSDDLTVKLWNWEKNWTLEQTFEGHEHFVMCVTFNPKDPSTFASGCLDSTVKVWSLGQPTPNYTLTTGQERGVNFVDYYPLPDKPYMITSSDDLTVKIWDYQTKSCVATLEGHMSNVSFAVFHPTLPIIISGSEDGTLKIWNSSTYKLEKTTNPGLERSWCLATHPTGKKNYIAAGFDNGFTVLALGKDVPTLSLDPVGKLVWAGGKNASPSDIFTAVIRGNEDADESEPLLLQTKELGSVDVFPQSLVHSPNGRFVAVVGDGEYVIYTALAWRNRSFGKCHGFVWGPDSNSYAIVDETGQVKYYKNFKEVTSWSIPLTYGVEKLFGGGLLGAKADGFVYFFDWESGTLVRRIDINAHDLIWSDSGELVMIINSEENREEEASAYSLAYSKDVYDEAVAKGDVDEENGVDDAFEVLHELNESITSGKWVGDVFICTTSTNRLNYFVGGKVYNLAHLTREMYMLGYLARDNKVYLADREIHVYGFEISLELLEFQTLTLRGELEEAMGSILPSVESKDTLIKIAQFLEGQEYYEEALQTSPETDQKFDLALKVGQLTLAREILGDEDSELRWRALGDASLQRYNFKLATEAYSRAHDLESLFLLHSSFNNKEGLLSLAKDAETSGKYNLSFKAYFIAGDVESAKELLVKLNKYPEAALLAVTYGIETENVNHIVTKWKESLKAEGKNAIADRICFSDKENDQNKASLLIDIDSTPSENGEVAKQSEAAAPAEQSPQAEEETERSERPEAPVEQEEPETPEQETSKVEEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5G0</accession>
    <name>A0A8J2T5G0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Inactive metallocarboxypeptidase ECM14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Inactive metallocarboxypeptidase ecm14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_14598g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Inactive carboxypeptidase that may play a role in cell wall organization and biogenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5G0"/>
    <dbReference type="OrthoDB" id="3626597at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03860">
        <property type="entry name" value="M14_CP_A-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000060">
        <property type="entry name" value="Putative metallocarboxypeptidase ecm14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF147">
        <property type="entry name" value="INACTIVE METALLOCARBOXYPEPTIDASE ECM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035227548" description="Inactive metallocarboxypeptidase ECM14" evidence="13">
        <location>
            <begin position="23"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="14">
        <location>
            <begin position="121"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF88624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="431" mass="49887" checksum="E98A0D89B888B97A" modified="2022-05-25" version="1">MVAKLLWMVCWTILMILPHAAGLQRGYRDYRVCRFTTQNRTEVIDYLAKQVPGNYDVWARSSEFIDVRLPKSLGALEGCQVIIEDIDAAIEATMPQSSVAQEARDQVAFTSKNNGDFFFQEFRDLETIYTWYELLERTFPQLVKVETVGETWEGRPLHALHISTNNPDTNPEKKTIVITGGVHAREWISVTSACWTVHRLLTSYGKHNRETKYLDSLDFLIIPVFNPDGYAYTWQHDRLWRKNRQETYWPNCPGIDIDHSFDFQWKRSDEVPCTEDYSGEEAFEAIEAHSWNHFLNTTKLQYKIHGYLDFHSYSQEVLYPYAYSCDALPRDLENLLELSYGLAKAIRNKSGKHYKVLSACEDRGSDLAPGLGSGSSLDFMYHMRAHWAFQLKLRDTGNMGFLLPPRYIEPVGKEIYAAVSYFCSFILNPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5G9</accession>
    <name>A0A8J2T5G9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">BN860_08790g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_08790g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5G9"/>
    <dbReference type="OrthoDB" id="655540at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF692">
        <property type="entry name" value="TRANSMEMBRANE AMINO ACID TRANSPORTER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="433"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="471"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="526"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="549"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="584"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="212"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF88376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="607" mass="65795" checksum="12E5CFCED2AB28C5" modified="2022-05-25" version="1">MAGTSEEDPLRGENTNHSQVHYISIPVNENRTFSHILEDSSFPAGSFAPRRQSILDQPIGSFRGVNSLSRFATSLRRANSFRHIDVHPEVSRNYFEQSNDELVNPETMEPTHGGRRLSVALGRAGNLAIRPSIGNLDDRRITGHTLQDAEDYGGTAVSVTSDNHSQFRDGASVGESIVVPGATGVGMGDNESITLRKIEGKDGEIVTVLAGKSTGPQTIFNSVNILIGIGLLALPLGLKHAGWIPGLILLTLMAFATFCSAELLSRCLDTDPTLMSYADLGYAAFGSRGRALISCLFTLDLLGSGVSLIILFGDSLNALIPKYSSNFFKILGFLAVTPGVFIPLSILSNISLLGITSTIGTVVIIALCGLAKREAPGSLIDRMPTELWPENFRSLCLSIGLLSACWGGHAVFPNLKSDMRHPAKFKDCLKTTYKITAITEIGSSIIGYFMFGSIVDDEITRNVLLTEGYPNFVYVLISALMSVIPIAKTPLNARPIISVLDTIMGIEGEECKTDHPSSRTTKLIRFFNRIIVNVLFVVIAILFPKFDKLIAFLGAGLCFTICLILPCLFHLRICNLTIKWWERILCHITIYVSICLATLGVGAAAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5H4</accession>
    <name>A0A8J2T5H4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pumilio homology domain family member 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_08240g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PUF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5H4"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07920">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000004">
        <property type="entry name" value="Pumilio homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUM-HD" evidence="9">
        <location>
            <begin position="511"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="532"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="568"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="604"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="640"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="676"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="712"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="754"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="802"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="45"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="294"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="367"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="424"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="294"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="442"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="452"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00081811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF88353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="874" mass="94903" checksum="FE2DD1D4C7ECFFB7" modified="2022-05-25" version="1">MSVMSNADPWFSSVTRLEEDGDHPSKSTNNMDAELASIVSSLSALSNPAHNSTSDRRGSTQLGGFRRPSLSYGGPGTTTAAPPAAAGNSQQHTTGAMGPMGMQDDASSQSSSPIVARRTALSVGTAPPYNGGYQDETSSLSGQRLFGATGRNSYNNRMNFMGHYSASIAGPILFNHSSSTGDGSGGGGFFEKFGKSLIEGTRELENSSSSDNVNHEVSGHGSPEGLSVPDQLLANPVLERRRASVTSNSTASESVEQFQEAAGKSTRNIWNVANAPVFKPIPNGHGDQFMMSTFPPQSQQQASQGSPFPPPFMPQFLPQTLASPPIPPNPVSATASPPSAALPLAQNPYLYMGGDLYHGIPSPQVQEQLPQANEPPKLPPQGVATGSAPDTNANVSAATTASLGVNANTGVNARVAPTIQGKRSIVGKSSSKKLQANPYLDNTNNSSTSNTHGPNGNRNSSASPHPFHGKATFSNKKKNNMANKVAPPTSSHGKVKNGGSNGAKWSSYYTVRSPVLDEFRTNPTNKVYSLKDIVGYVLEFCQDQHGSRFIQYELASASPSEREVIFNEIRDQSVELSDDVFGNYVIQKFFEFGSKTQKDILVSQFKGRMQELSMQMYACRVIQRALEFIDLEQRIDLVKELSTCVLEMIKDQNGNHVIQKAIERIPMVELPFILDSLDGQIYHLSTHAYGCRVIQRLLEFGSEEDQTRIFREIKDFIPFLIQDQYGNYVIQHVLQQNGDKCLQDMAETKQNIVNIVSQSVVEFSKHKFASNVVEKAILFGTKEQRDLIMSKIMPRDEANAANLEENSPMVLMMRDQFANYVVQKLVGVSEGQEKRLIVIAIKSYLEKLSNSEAVGNRNLASVEKLASLVETVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5K8</accession>
    <name>A0A8J2T5K8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S04-07492g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_07492g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6407164at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000400">
        <property type="entry name" value="Negative regulator of differentiation 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
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        <property type="entry name" value="mRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018835">
        <property type="entry name" value="RNA-binding_domain_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF10">
        <property type="entry name" value="RNA-BINDING PROTEIN NAB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10567">
        <property type="entry name" value="Nab6_mRNP_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10378">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="605"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="106"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="415"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="524"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="826"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="864"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="977"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="106"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="415"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="525"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="828"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="864"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="894"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="986"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1027"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1069"/>
            <end position="1082"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF89572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1140" mass="126977" checksum="45D73A3AFCC24A0A" modified="2022-05-25" version="1">MQRKGSGDGLFYQQYPGQLNASYRPRERDQETARVMANSPMAYPPVGSLQLQYGGPQQAYYAAAGPPLMNTPPTPFDTAYGITLLPSHLLIGSPYVSSPDLFQSHHQAQNQGSNMKHQSAVQRQRRPPRGSGGSRLSQAYPVAPPLKNPPALARNRHRRRAQHEQASKMFQEPFVVSYKMLPKGDDEYRTRSLLFENVNSSVDLYSFVSKYSKYGPVESIYMFRNRADKSKNSVLLSFLSRKICLDSYNSVLQRLAEFKTGLKSKSLTVSFVSLKYKRHVEPEEEDQIGEDNDPGDGDDAYNVSVVASLQYDIVNRGATRSLALNLDDECDKDELLKNRLDFLSPSKNQRYVLESIYLIKAKEATRHFPKNYAILTFLNISMAVDVLDYLRNSEQNFESSQCFFVSVSPHSQPSFESNTSFDHTGGAKNNSAGGLVGNAPNGNLGSYTSKNASLSSLNSFGSYISLDQEIDLLATSLQGSHTVIVAVDHYPQPFFAEYTDHLSNASVSEASALDTQMLMPRPQEMTVQSQDSPVGPSSNAVNSGTTSLSTQVLHSQMEIGSGMYMEPFGSRSNQGIVKPITQSLQKQFATSAEVANSMGGGIGNRTVYIGNIHPRSRAEDICNVVRGGILQSIRFLQDKHICFVTFIEASTAVQFYTNAFIDPIVLHGNMLKVGWGHHSGPLPKSISLAVTVGASRNVYVSLPDFAFKDKYINDPQYQKYNGKFKLPTEEILRKDFSQYGDIEQINFLSDGHCCWVNFMNISSAIKLVEEVKYNDGEAFYKTFDDRYRGLIIGYGKDRCGNVNKNLIAGKNSRFYKKVKKPSYNIRLSRMEEKRRKHEESLRLQNQSNSEKLLQFGSLGITLEGKDERRESKGSNSQSDDKESALVHELDHSQEQSSESGQQQVSGEKYDEEEKELLKLSNKSQILDGLGIAAAVAEEDKDRTVAKNEEILHRACESDDEESLDSGVSSNVELIIHSPLGSQSDLQKSDKPERTSKKYHGSSYASMTEGASAVSNPSLRMNPPMAPSTISRNYSLMMKSQNGHNSHNRQQNQGFSAEDHASSSQGGRSKGQRNRRRNKRRSAKTIPGSDVMAQYLAQLQHSTFMYAANILGATAEGDVNYYDDSAXXKKKKSMNFNMNER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5L7</accession>
    <name>A0A8J2T5L7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_15698g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5L7"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000949">
        <property type="entry name" value="Serine/threonine-protein kinase PTK1/STK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF32">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="191"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="503"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="570"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="602"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF88670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="618" mass="68812" checksum="0F6DCE74E8FC16F8" modified="2022-05-25" version="1">MSQRTESMNPFKALGKKLKGHSDHILNGGSKAGSPEKNGRDRASSLVSRRIPPGGHHPHVLKTAESSPALQNMMAHNHNPFAHANPQLSHARGPLRRRTHTNGKKNNNSPRNGSHPERMVYNPYGLNNSHSGTAQGGAMNNGGGRNDLSFYMHDGDAHVRVLPLPIADPNAYLPDDMKQISISLTDNFVFDSDNQALGSGGSSEVRKIRSSHHQRDVYALKKLNMIYDETPEKFYKRCSKEFIIGKRLSHNVHVANTYCVVKVPTTTYTTRGWGFVIEICERDLFQLMERSGWRGVPLPEKYCLFKQIAEGVKFCHSQGIAHRDLKPENVLLTHEGVCKLTDFGISDWYHTDPADPSSPVKRCEGMIGSSPYAPPEVMMYDAKKCYPESLQKPFDPLKMDTYALGIILLALVQNMLPFLESCDVDSRYREYAAGYEQYVQYQNPHFRDKGCHRPGPGSEYLLARNFNNSDAARVAWRLADPNAATRYTMEDLYDDPWFQSIETCVDPDEPPKLNAPDLRGPRGQSDESLASSKPRSMVDIAETPSQQQHQQGAAHVTRKMSELSMTSAGSPPAQQQSVPVAQQNSSASRAKKRRVVHNHMDVPASVAPSASARAMSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5M6</accession>
    <name>A0A8J2T5M6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_15940g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_15940g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5M6"/>
    <dbReference type="OrthoDB" id="4065296at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="582"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF88681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="667" mass="72152" checksum="3E5CC72E18993F60" modified="2022-05-25" version="1">MDDPVVAASAGAETESVSADMQAFKQNSTDEIAKFFEHIKNKQGIFDKQEHSGLSTESGIPATLSSSEPAELVVQKYDVDYKPKLSVNKKYVYSINELISISRQLPAGYAEGIVAYLPKKKFWRLNHRQPEHAGQSKSMGRRGSNKGLNGSGSGKGLSGDENNVFEKKGHKGKYARTPNGKKGNKFGKHDRGAYLEEKDVAVNNDDLQALEEEFEPTGNSMADFESWKAKMKEMERKKKGLPPIDQSTSSKPSLTKASSSISDFLGLNRINSDGVANNMEFQDVYKKTADDNNTIYKEAQIESAKGSYSRFSSFFAQSSSSSSLPTEKNTNVESEPLSVQNTESAPNSQNPQVPGGSRLLSFFKNESNTPKDSKNQSSLNSQEVGQNPMDSKIVPSHLSQVSHNSHSSHGSRGSDGLNVPHGIPQQESRTMPPPALPSQQTIAPDQLQTNNAFFQGLLNKGDLQNNDRNVQGPPPGVMLPPGGQTRFTLGQQPSPPPPHTRQPPGINVSRQFPVGMPPGSGGFPAPTPHPTFQSNGVQGVRHSDAPTMMNKNVNNVSQRNTPSGSNSNGGTAEGRGLQHSQAPPPSFQPGMVPPHFMPGMAPPGFPMMPPNFNPMYIPPQGGMMPPPAGQGFFPPMPPPSGNMNFNAFPMRPPMPQKQEAKATEIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5P6</accession>
    <name>A0A8J2T5P6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="16">BN860_11804g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_11804g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17993">
        <property type="entry name" value="DEXHc_CHD1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000038">
        <property type="entry name" value="Chromo domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000007">
        <property type="entry name" value="Chromodomain-helicase-DNA-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000130">
        <property type="entry name" value="Chromodomain-helicase-DNA-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041150">
        <property type="entry name" value="Cdh1_DBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056302">
        <property type="entry name" value="CHD1-2/Hrp3_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025260">
        <property type="entry name" value="CHD1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF14">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18196">
        <property type="entry name" value="Cdh1_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13907">
        <property type="entry name" value="CHD1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23588">
        <property type="entry name" value="HTH_CHD1_Hrp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01176">
        <property type="entry name" value="DUF4208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="13">
        <location>
            <begin position="187"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="13">
        <location>
            <begin position="277"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="380"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="691"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="133"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="967"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1244"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1037"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="54"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="90"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1282"/>
            <end position="1314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF88505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1423" mass="163677" checksum="AE216618089E1075" modified="2022-05-25" version="1">MAKDIDDEVLENPELYGLRRSHRAAAHLPQKYFSDDEEEEPVIGSRRRKKQADPDAEDDELEDEEMEEDDNSEEDIEEDEDDDYFGSPMKNKRKARKNHNKRGRPSKVVDEKIPTRFSNRTNKAVNYNIDYSDDDLLESGDENAETGIDGSDLEEGDEPVYSTTPQLEEDHGVDLVITHRLREGVDPLMEKSVPDLNQCKKNYEFLIKWSDQSYLHNSWENYEILRQIRGIKRVDNYCKQFIIQDLELRLDPYVTAEDLEVMDMERERRLDEFKEFEKPERIVDSSRVTTEDGVSQLQYLVKWHRLNYDEATWENATEIVKLAPEQVKHFQNRANSKILPQYSSTYSSQRPRFEKLSVQPPFIKNGELRDFQLTGINWMAFLWSKNDNGILADEMGLGKTVQTVAFISWLVYARRQNGPHLVVVPLSTMPSWQETFEKWAPDLNCICYMGNQRSRDAIREYEFYTNPQAKGKKNVKFNVLMTTYEYILKDRSELSTIKWQFVAIDEAHRLKNAESSLYESLNSLKVSNRLLITGTPLQNNIKELAALVNFLMPGRFTIDQEIDFENQDDEQEQYIRDLHTRLQPFILRRLKKDVEKSLPSKTERILRVELSDVQTEYYRNILTKNYGALTAGAKGGHFSLLNIMNELKKGSNHPYLFDSAEERVLEKFGDGNRSRENILRGLIMSSGKMVLLDKLLTRLKKDGHRVLIFSQMVRMLDILGDYLSIKGINFQRLDGTVPSGQRRISIDHFNAPDSNDFVFLLSTRAGGLGINLMTADTVIIFDSDWNPQADLQAMARAHRIGQKNHVMVYRLVSKDTVEEEVLERARKKMILEYAIISLGVTDGNKYNKKNEPSAGELSEILKFGAGNMFAAKDNQKKLEDLNLDDVLNHAEDHVTTPDLGESHLGGEEFLKQFEVTDYKADVDWDDIIPQDELKKLQDEDQKRKDDEYVQEQLQMMNRRNNALKKIRDSVNGDGSGHVSDGEEGGTKAAKKRDKVMNMEDLEEREIRALYKAILKYGDVSEMLEMLIADGSLPDKPIEKYEEAYAGMIEDAKEYLEKEERKRSQVMENLENEARIYRSKLKSGEIRADDQPKVSPLTKLAAKRKEKKAVLFQFEDMKNLNAESLVNRVEDMKLLKSYMNKHFKDDALKFRFTSQQPKAVQNWNCHWDKTDDEKLIAGVYKYGYGSWAQIRDDPFLALENKIFLNDTVGNTESSDKKFKSSKKVPGSIHLGRRIDYLITVLKDEASENDNGPKRRKPSSKAGSPDTGSEQPAKRMKPLPKGPASMSPSRATSSSSANGKSRPPKLSNSNSASNSSMTSVPPVHVKEYDSMDEDDCMRAMGAMRVSLKRLRRGGKGLDRKEWAKILKTELTAIGDHIESQKRSNTSNPEKLRKHLWSYAANFWPAAVKSSKLMAMYDKIRGSQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5Q3</accession>
    <name>A0A8J2T5Q3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ZYBA0S03-01178g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_01178g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18597">
        <property type="entry name" value="ABC_6TM_YOR1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18606">
        <property type="entry name" value="ABC_6TM_YOR1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001750">
        <property type="entry name" value="ATP-binding cassette transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000010">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="320"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="458"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="882"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="922"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1002"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1099"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="199"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="563"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="883"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1195"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="539"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="790"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="539"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF88753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1459" mass="165613" checksum="415C8613D958838D" modified="2022-05-25" version="1">MSEEIKSVQHEVDGALPGSCSSEFSNSNSGLHSDESSDIQLGPAGDYIVKRNRPETFLNSDDLEKVTESNIYEQKRLFSFLHGKKVPPIPQDDSERDVLPFFSANIFSRIFIWWVTPLLGKGYKRTLQPNDLFKMDDRLSVEVMYARFEKNLAIFHERGRRNYRKEHPDASEEEVRDNYKLPRLSLFLTLILTFKWQYLWSCLCSVLANCASGFNPMLTKRLIEFVEKKAVIHGLHVNKGVGYAVGASLIMMLNGLLFNHFFLSSQVTGVEAWSVLTKAMLSKMLRASNYTRHKFPTGKVTSMVTTDVARIEFALSFQPFLAGFPAALAICIVLLIVNVGPIALVGIGLFFLVFFVTMFFFKYIIKMRIASNILTDARITLMREILNNMKMVKFYAWEDAYESNIRDVRAREVSRVRRMQITRNFLIAMMIALPSVASLMTFLSLYKVNNNGRTAGNVFSSLSLFQVMSIQMFFMPIAIATSIDMMLGLQRMERVLESSEESTDITRDMAPSPDLPENIAVKMSHASFEWEDYEKIDMNKEEEEKKEEKKEERKEEQPFENSTNDTDEAKPGALEKKSFTGFYDLNFEIEKGEFIIITGPIGTGKTSFLNSMAGFMSKTSGDVEINGKLLLCGYPWIQNATVRDNIIFGSPFDRAKYRKVVTACSLDADLDILPAGDLTEIGERGINLSGGQKARINLARCVYKNKDIYLFDDVLSAVDARVGKHIMDECLVGLLDGKTRILATHQLALIEKASRIIVLGNDGSFTIGTYSELKDTNATLMNLMQFTSQASEEDRRHENAELEKADMEEKEKVQELLEYKDEEEEQWEDLKKQVTQKSLTMKGDGHTTTKEERAFNSIKLKIYVEYIKAAAGKWGWAAIPTYVLSIFGSAFCSVFASVWLAFWTENKFKNRRPSFYMGLYSFFVFGQYLFMNIQFSILCFMGLTASKWLNLGAVKRILHTPLSYMDTTPIGRILNRFTKDTDSLDNELTEDIRLMMYQLGNIVAVIVLCIVYLPWFAIAVPFLLIMFFIIADHYQSSGREVKRLQAVQRSFVYNNLNEVLGGMDTIKAYDAQQRFLLKSDFLINKMNEASYLVSALQRWVGIFLDILAVAFAFIITMLCVTRQFKISGSSTGLLLTYVLQLPGLLNTVLRASTQTENGMNSAERLVSYALDLPQEADYRRPEMAPPEEWPSKGEIKFEDVSFSYRPGLPPVLKELNLDIRSGEKIGICGRTGAGKSTIMSALYRINELNHGRILIDNINIADLGLYELRSKLAIIPQDPVLFKGTIRKNLDPFNEKTDDELWQALVRGGAIAAEELEQTKLDKPDGSGSHSKMHKFHLEQKVEEEGSNFSLGEKQLLALTRALVRNSKILILDEATSSVDYETDDKIQSRIVEAFGDCTILCIAHRLNTILNYDRILVLERGEVAEFDTPWNLFIKEGIFRSMCARANINEADFKNASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5Q4</accession>
    <name>A0A8J2T5Q4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HTH CENPB-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_12178g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5Q4"/>
    <dbReference type="OrthoDB" id="125347at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050863">
        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006600">
        <property type="entry name" value="HTH_CenpB_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303:SF73">
        <property type="entry name" value="PROTEIN PDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303">
        <property type="entry name" value="TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03221">
        <property type="entry name" value="HTH_Tnp_Tc5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00674">
        <property type="entry name" value="CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51253">
        <property type="entry name" value="HTH_CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH CENPB-type" evidence="3">
        <location>
            <begin position="63"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="631"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="631"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF88520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="812" mass="92273" checksum="44088564F35BCA7E" modified="2022-05-25" version="1">MLSIEHRYNICLMAERHPKWTQLELARWAYEAFQLPKVPSQGTISRLLAKKDIYMNCKDSEKCANRIRKPNNLLVRRILQEWVSQSIWNGIPVTSPILQDTAQSVWYRIPAEHREGNGCFSYKWITNFLAKMDVNISNMDEETPKPPKVWTFEERNTLKEYFRRVPPKDLFTLDETFLAYNLPLDYAQYEASQMQRRIEVATVMLCCNLDGSEKLKPLVVGKYNSYKSFRNYFPEEPQDPVSQSLLGEKMARKFGISYHSNRKSWLTSNLFHDWLARWDKRLVADNRKIWIVLDDSCSHRIINLHLKNIELIYTSANSRFLPFNWGVLDEFKTRYRIQQYQALIDLQNQLEKKTHRKMLISFEQSQLTMSNAFKFIKKAWKSIPLETIRGNWKSAGILPTDMVQIGESVSMAFKKNEVLEAKLNELCSQFYCAKKWDHDMLLDLNIENKNTNFLSTEELVESAIVEPMEPDTQDERTGNSGGPNSMWCYQNLELIADGNVGINVGDIADNSESDDEDMENNSNKNDIQHVEQAIDSDINDGSVTIANGNGAGEIDAAYGVNIDKILADDYPKDNQGRVYNVSTLIDRPNLFMGENGELDLRNVGLDPSIVSSEYFNDVFSSAVADQRHATVQGSNGTNHGGMPTQNLGSSYPNRTAEPGGASHSQVSSTTPMPPFEDPTLNHILNDAVVTNRLRGSSPLSELPLDTTSAQNTQTNINIAHALGSVLKHVESRELVFSETALNEIKFNYSALLSKIRKTRKNTSISKARRGQVQLERFLTNTSNPSPTDSDISTTQHIQGPVNLQMPGNASFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5Y6</accession>
    <name>A0A8J2T5Y6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_14950g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_14950g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid/choline transporter (ACT) (TC 2.A.3.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5Y6"/>
    <dbReference type="OrthoDB" id="4476201at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000046">
        <property type="entry name" value="Amino-acid permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF6">
        <property type="entry name" value="GABA-SPECIFIC PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="375"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="422"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="493"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="523"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="578" mass="62247" checksum="4401C6E474553EBD" modified="2022-05-25" version="1">MSSSSGKASMQADKIAIQPTLSAAEGSTFELRDMGSQNANIRSIHSKTGAGEVNYINAATSHSDNQLLAEIGYKQELKRQFSTLQVFGVAFSIMGLLPSIGTVMGGGLDGGPVSLVWGWLIAGFFILLVGITMAENASAIPTAGGLYYWTYHYAPVGYKEVISFVIGCSNSLALAAGVCSIDYGFAEEVLAAVVVSKDGNFDVTNGKLYGVFAATVVAMAICTCLASSAIARMQTLSIVSNLFIIILLFIALPIGVAVNRGSDQFNGGKFIFAKYENFSDWNDGWQFCLAGFMPAVWTIGSFDSCVHQSEEAKDAKKSVPIGIIGSISACWILGWLIIICLMACMKSDVDAVLNTKYEIALAQIIYDCLGKRWTIAFLSLIAFCQFLMGASIVTAVSRQVWAFARDNGLPLSRYIKIVDKRYSVPFVAVIFACAASLALGLLCLQGQAADALFSLAVAGNNLAWSTPTLLRLTSGRDIFRPGPFYLGKILSPIIAWIGVFYQAFIIILVMFPGNQHNVTGPTMNYACVIGPGVWILSWIYYITYKKKYFHGPASNVSEEEYVEAVGEDAIDSILSKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T674</accession>
    <name>A0A8J2T674_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_18162g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_18162g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T674"/>
    <dbReference type="OrthoDB" id="286814at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF36">
        <property type="entry name" value="PHO85 CYCLIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="1">
        <location>
            <begin position="72"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="192" mass="22390" checksum="FDC68B4EC593A4BB" modified="2022-05-25" version="1">MTMQSPDPKQVIRLENLACFAYQTPPYKDYGSDDACTKGPPCNNAFNELLVDKLATLLQMHTMKCDVSRARRGTKSLRDFLKELLKRSQCNRRASLLAAVYFERIYQVSDELPEFGRCPRRMYLCCLILAHKFLHDKTLSLNAWRLISGLKPRELSFMERWCLGRLDYKLNIKNKELRALESKLLKLTKSHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T688</accession>
    <name>A0A8J2T688_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BN860_18558g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_18558g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T688"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015793">
        <property type="term" value="P:glycerol transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000061">
        <property type="entry name" value="MFS sugar transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF55">
        <property type="entry name" value="SUGAR TRANSPORTER STL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="450"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="513"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="545"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="121"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="611"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="613"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF87966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="659" mass="72942" checksum="400E5A76CAA99CC1" modified="2022-05-25" version="1">MAGSISERDITPNNSNTMAADLEKASPIGSRSESDTGFEENESSTPRQEGDVNKEYDDYAASVTGDMPHSGDSGIKTQSAPGYDYNNEYENGFEPPRGIKAYFMSRTSTAGLQGTKLRVACAGTAVIAFLLFGYDQGLMAGIISGPKFNEEFPATKEGATHKRTAAANQAAVTACYELGCFIGSVFTMFFGERIGRKPLIIMGSLITIAGAIISTVSFGHHWGLGQFVVGRVITGAGTGLNTSSIPVWQSEMSKPESRGRMINLDGSTIALGTMIAYWLDLGFSFINNSVQWRFPVSLQILFAIFLFIGIIQMPESPRWLIAQKRVQEGKYVLARLNGCSMDDDAIIAEVTMLLDTARRFENKKRSIKDLFTNGKTQNMNRAIIASSTQFMQQFTGCNAAIYYATILFENTLHRSRTLSLIIGGIFATIYFLSTLPTFYLVEHLGRRKMFLIGVSGQCMAFIITFACLTHPSQMHGRGAAVGLFVFIVFFGLAMLPLPWIYPPEVASMRVRAATNALSTMCNWLTNFAVVMFTPVFILTSQWGCYLFFAIMNFLYLPIVFFFYPETAGRSLEEIDIIFAKAHIEHTRPWRVANNLPKLSMKEVEDYSNALGLWDDENEKEGIEGDENEEEPQENALFTAEPLHPSSSSSSREYEQAPHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6A4</accession>
    <name>A0A8J2T6A4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Holocytochrome c-type synthase</fullName>
            <ecNumber evidence="10">4.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_18910g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Lyase that catalyzes the covalent linking of the heme group to the cytochrome C apoprotein to produce the mature functional cytochrome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>holo-[cytochrome c] = apo-[cytochrome c] + heme b</text>
            <dbReference type="Rhea" id="RHEA:22648"/>
            <dbReference type="Rhea" id="RHEA-COMP:10725"/>
            <dbReference type="Rhea" id="RHEA-COMP:10726"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:83739"/>
            <dbReference type="EC" id="4.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cytochrome c-type heme lyase family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.17" evidence="10"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6A4"/>
    <dbReference type="OrthoDB" id="4243at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004408">
        <property type="term" value="F:holocytochrome-c synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000511">
        <property type="entry name" value="Holocyt_c/c1_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12743">
        <property type="entry name" value="CYTOCHROME C1 HEME LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12743:SF0">
        <property type="entry name" value="HOLOCYTOCHROME C-TYPE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01265">
        <property type="entry name" value="Cyto_heme_lyase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00821">
        <property type="entry name" value="CYTO_HEME_LYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00822">
        <property type="entry name" value="CYTO_HEME_LYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0349">Heme</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF87981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="260" mass="29869" checksum="67A02E0DF42D27EE" modified="2022-05-25" version="1">MSSPSDKSSEAKCPVDHSSREVWLKQAQQQKQEQKCPVDHNSREVWMQNAQRTQPLEQKCPVDHKTQDVWLQQARGVGEAVECSSDELPTIPKYNTDVELPENREVSSIPRTGAQANWVYPSEKQFYEAMLRKKWDPDAIDMRTVVPLHNSVNERVWNCIKSWEKDQGGDACGGVQLTSFKGDSKKLTPRAWFRSTILGLSKPFDRHDWTVNRCGKNVDYVIDFYNEDDESMGPQIFLDVRPKLNSLEGVRLRVLKSLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6B1</accession>
    <name>A0A8J2T6B1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Very long-chain fatty acid transport protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Very-long-chain acyl-CoA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">BN860_08306g</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Acyl-CoA synthetase required for both the import of long chain fatty acids (LCFAs) (C14-C18) and the activation very long chain fatty acids (VLCFAs) (C20-C26) by esterification of the fatty acids into metabolically active CoA-thioesters for subsequent degradation or incorporation into phospholipids. The transport and fatty acyl-CoA synthetase activities are genetically separable and are thus independent activities. Esterifies VLCFAs in the peroxisome matrix. The VLCFAs are actively transported into peroxisomes by a PXA1-PXA2 heterodimeric transporter in the peroxisomal membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54536"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58950"/>
            <dbReference type="ChEBI" id="CHEBI:138261"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6B1"/>
    <dbReference type="OrthoDB" id="10253869at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044539">
        <property type="term" value="P:long-chain fatty acid import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000020">
        <property type="entry name" value="Long-chain fatty acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000019">
        <property type="entry name" value="Long-chain fatty acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107:SF15">
        <property type="entry name" value="FATTY ACID TRANSPORT PROTEIN 3, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="13" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="21">
        <location>
            <begin position="83"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00068795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CDF89064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="670" mass="77134" checksum="D5780EA16DCE5E8B" modified="2022-05-25" version="1">MSKNAVSQLLLRALLATFRLCKALLLLLFGFLSWCFSSTIEYLDRRYRIKEDFHIVPFFLKSVFCYCIDLKSHKFQYWYHFVKQVEQNGDCMAISYPRPLAKKGDFQLESYTYKETYDIVLRLSYVMYQHYGVRAGDNIAVDSTNKPLFLFLMLAAWNIGAVPAFLNYNTLGKPLVHALKISSISQVFIDPQASGPIKSSEKIIQEELPDVKLHYLNENDLMQSLQDPQFPQFLQEEDVRSPQDLTDYRPAMYIYTSGTTGLPKSAIMSWRKAVIGCKLFARILHMDNQSIVFTAMPLFHSTATLLGVCAVWSAGGCVAISNKFSASNFWKEAYLTNATHAQYVGETCRYLLHTPVSKYEDMHSVKVAYGNGLRPDIWQKFRNRFHIEVIGEFYAATEAPLATTSYQKGEFGIGACRNYGSIVQWFLSFQQTLVRMDPEDDSVIYRNSKGFCERPAVGEPGEMLMRIFYPKRPETSFQGYVGNKKETESKVIRDVFRKGDAWYRTGDLLRADKYGLWYFMDRMGDTFRWKSENVSTTEVEDEIMNSNQEDFDQVVVVGIKVPGYEGRAGFATIKLNNPGIPERRKVAILNEMLIHLTRELPKYAFPIFVKLVDTIEMTDTNKVPKKKFRNQVLPHGANGKDTIYWLKDYKEYKKLTSKDWAAIESQDAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6T9</accession>
    <name>A0A8J2T6T9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ZYBA0S03-12090g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_12090g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6T9"/>
    <dbReference type="OrthoDB" id="1914839at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd24142">
        <property type="entry name" value="ACL4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF89223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="379" mass="43331" checksum="D2B276C7ACA00E94" modified="2022-05-25" version="1">MSELESALEQTRRFLSIHDAKGALKVLKPYKKSLKSKNANNVILHQVFAEAYLEDGQLEKAYPLLTRACELDIYGEQGGSEKFFILGQATGGHDGVKLLFQGIENISKKADNTIAQEHVEKIIDGLLTAIEIWMTDLCMEENAESQCEELITKAMEISEGQSPEVWSMLGSIRISQQRYRDACHAFEYSWKFFEIKKQHLEQTINLNQAPCHEEYVQLVQPLLTLSKMCIEMGLYEVSMKILVSIKDIDEDNMESCYLEGFTLYLMSKLELFRQSHPEIHLNADNIYEFNEHFRELPLDLTNESILETIREARLALSISVKLAQNADSNDEFARELLEGSNEVLSEIGGPLDDSELINIRKGDENVALEDKDFEFEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T738</accession>
    <name>A0A8J2T738_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BN860_08966g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_08966g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17327">
        <property type="entry name" value="MFS_FEN2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000482">
        <property type="entry name" value="Thi73p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF40">
        <property type="entry name" value="THIAMINE PATHWAY TRANSPORTER THI73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF88384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="539" mass="60861" checksum="F8BE52891CB7196A" modified="2022-05-25" version="1">MQPEEVEKSKAELEVVSSTDAVASEDAVTSDDDRADIALQFLNNTHAKEYVLADPDEEDHSKNGKRIFYGSTELPPKILRKIDACILSFLCCTYLLMFLDKALLNYAASMGIKDHLKGNDFSNLSTIFSAAYIFMEPLVTFLIQRCPISKVMAVFITCWGIVLACHSACKSYASLMVVRTLLGIFESSSAVGCIAISGMYYTKAEQCARIGYWATQAGTGYIIGGLISFGFLHYHGTTFTSWQIMFLVVGLVTVVFGLLTWVYLPDNITNAWFLTQEEKVLVVKHIRDNQTGLENKKFKWSHIKELFCKDKLTWPMLAITACSQMSTGAIGTFSVTITETFGFDKYVTSLLQLPIGAITAIIILVTTQMMSKWGQITLITTSMYIPAIIGCIVMLSLSLSHKIGNLFSLYLLYSGSCVITNIYLWNSCNTSGYCKRIFRNAATMITYNVACILAPQMFRENSYPRYIPAKITLLVTQCVCIPLQLYVGYLSRKENHERDKEQENTETQAYQFLDLTDIXXXFFFNWHLLMIFFFYVLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T775</accession>
    <name>A0A8J2T775_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA repair protein RAD5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">BN860_10440g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T775"/>
    <dbReference type="OrthoDB" id="2801544at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014905">
        <property type="entry name" value="HIRAN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF22">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08797">
        <property type="entry name" value="HIRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00910">
        <property type="entry name" value="HIRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="555"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="948"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="1027"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CDF88445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1201" mass="137668" checksum="270EC80FCF267C61" modified="2022-05-25" version="1">MNQRSFRGLKACKKAVRKQVAHEYRPSGMGYGRKRYFRDEFDVSQRKDESEFAVNGDSFLFKSGEVEQIEPADPVNPTDLADQAGSEKLKEPMDEVVDVRRNSFSNEKERTYEDLQDGLQKAFQRAPGYPDYVTQIRQIIPGMSLEVAMKLVEKYQGEEDLLSKAVSSYFEEPIEAERTAKRSVRTVSSPQLSQKKTRREVGDLTSAQYHSNKCKQNFLWKKFIGSLQVNAMATRPTLKPLKYGTKLEIIQSSGELPISKLYNSNGRKKVSMASFVKIYDSAQKREIGRIPEDIAQVLYPLMHTNEVDFEVTMVFCDNKRLSIGDGFILQLDCFLTSVIFDNPSREPTPSQSVRWGGGTKLVETEEELRSRSRRVGLISLFDKLRLKTAVSDVDGADCEQEDTEVIRLDDEDDDTSTLQDIATQQGNNSMTSSQHEDVMDLNQLKVFYKATLSSDSLKSIPETEPPASAMKIILRPYQKQGLTWMLRREREFEKASESQGAQDVGSSMMNPLWKRFKWPRDMSWGAQRIAGYSEVDKGNFFYANLHTGEFCLDKPILKTTMRGGILSDEMGLGKTISALSLILTSTYDSSAVDRSPAVIEKSETTRLPSFQTFKKPYAAKTTLIVVPTSLLTQWQSEFDKANKSPDLYCEVYYGGNVNNLKTLLTKAKNPPSVVLTTYGIVQNEWSKMVRMDRRSAEESTGLFSITFYRLIIDEGHIIRNRTTSTSKAIMEIFSKCRWVLTGTPIINRLDDLYSIVKFLRLEPWSQLCYWKMFVSDPFERKDYRQAFDVVNAILEPVFLRRTKQMQDINGRPLVTLPPKEVVVEKLSFSNAQGAIYKHYLEKAENSVRKGLARGDLLKKYSTILVHILRLRQICCDVELLRRHARDENDEDLTKESQVFNHSDDANKIFQEIGLDKEVEEIDETLLCTVKSRIEGKYSSSSSLKNLECSICTTEPIEVHKILFTECCHSFCQDCLMEYIEFQKQKNLALKCPNCRETITSIHLFSLKLKDNQIPLVVPFRNVTRSSKIEALLKHCKMLQETSPGEQIVVFSQFSSFLDILETELGETFSADPIIVYKFDGRLSLKERANVLKEFSRKDFDNQKVLLMSLKTGGVGLNLTCASYAFMMDPWWSPSMEDQAIDRIHRIGQINQVKVTRFIIENSIEEKMLKIQERKRTIGEAMDADEDERRKRRIDEIQMLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T786</accession>
    <name>A0A8J2T786_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S06-03158g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_03158g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T786"/>
    <dbReference type="OrthoDB" id="160374at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052609">
        <property type="entry name" value="Ribosome_Biogenesis_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018849">
        <property type="entry name" value="Urb2/Npa2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15682">
        <property type="entry name" value="UNHEALTHY RIBOSOME BIOGENESIS PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15682:SF2">
        <property type="entry name" value="UNHEALTHY RIBOSOME BIOGENESIS PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10441">
        <property type="entry name" value="Urb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolar 27S pre-rRNA processing Urb2/Npa2 C-terminal" evidence="1">
        <location>
            <begin position="934"/>
            <end position="1140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF90205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1143" mass="131377" checksum="7EA51E4B1B5905DA" modified="2022-05-25" version="1">MLPIPESTEGLTKLLRSKSITPAQIVDIASRFDELDLYFPNKNIFIIELIDDRWNDQKLTKFKQDFKIWKLYNDFFSSLTDATIQKKLLKDLKFIPHLLATLNLVDFNISEFLEELKRTCILVNSLIKIEVSLDNAISILGKTLGLIQQTNYSHEDLIYEIIHLTDISNTVTVSTKASKWYCNELLLPTIKHFVKFGSNVTILSELLSRFLFDALIDPVKQLEEFVRTTNTSLEPADCVLLFEKSIYFLSKENFLKLEKIFVIITSIQKQLAPLLLNKLSVSKKTMSQEFLNELFNETIKNPVYDIEFWSLILHILNLDVEIGIEHFEQLIELISERKDVDPVFVENVWSRLIQCFVDAREYSGFLEKIRNYCTTHKKSSLFLLEEPGYSSKISRNVAILSTTQLKTIISSLIHEILASFMVHEESALILKLTLKGLTKLSYISLPELKPVVAKVFDVSAEHPPRLWEIWYLAMEIYDDIVPLNSLETIDDQISVFLPLKSKSKELFYYFFKLREYKDYNILPIVDEFLDFLRKSNEEAQKAALKDVFTKWCSLVNFTFPREALDILTGILVSGSNVELLGVIFEDDYIFEEPKIMFSLVQKLSELFESEIALSQIIQIPLQCINKNTRVALLNNVSSKEKITKLDTMLMGYLLENPTFKSHVETQMDYLYSMLSRTETLLTYENIAVEKVWQNHLNQMKEKGSITFIQESEKIIVNGMKECPDDTIYFQLAFLALKVCDSNALDSLKASFIQNCLKKLPCIVNNIRLLTWLLRVLYYTFKKDYSCIPKENSLLQNISALQKSIKPEDPSEDKDLKTSSFLLYSVLFNDKPEFLYAHYMVLRSDGIESDGILSAIEHSVKMCNLAQFNEVFANIVFSMSTCDLLFNEAILELYAMQIKHLTKNNSVGSHLFVKSISELYTNCENFRNARKPILQVLDAIQSLLISKQWLFSQYCVEMLFPMCLKIVLIMTKGAQTNDDIFICTNKIISNIFLAHGVKLSNRHHLVNSFLCASIELIADCENTGLSSLSAKSLSRLIINFVEPGINSSHNFSKKNALSSTIGLQKKLLRKYVPIILINYIHLSIFSQFGASAKKELAISMYSIFDLLSQAELNMVIGALDNAGRQYLRNLYSDYKKVGKWDSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7F6</accession>
    <name>A0A8J2T7F6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ZYBA0S04-10902g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_10902g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7F6"/>
    <dbReference type="OrthoDB" id="4064185at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF89714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="624" mass="71635" checksum="620DB4BB3D715790" modified="2022-05-25" version="1">MLGSFKRISRRLPTSLPRTRPVSTLPLPSANFNHLLQTISSEDRLAQQDKPAFKKYWEIVSENFPNARTLFKETDTDIFSQFVKNNRNHSSTVRAFYRREAIFNRDTLPTIQSLVGNKVYDDVLTFCIQDSISTRDIVIAADLFLLYYKLYHHEPIRHDILAEIISAIAFQHPKHDRLHLIKLLQLVKMVKSKKQQLKLDTTQTSALCDKALSLDNVPSLTKQVLLEVMSNSDNSCSIPKNTDLIAAYKLIHTDYTSRNAAGVYSTWINISNYYSSIADHDPRILYMVMRSCLNNKNYKAACKDIISRLSPNIYCNDPLILPTIIDYITKNADLSLAEDIMNNVNEHIKPNNTQNGLLSKRFLSSLLKMHLKFNDSKGVDCVLKQIHDTFGAYSQENYQAIVSHLLKTKTLDNIVKAVNIVSTIPPKQALLAYSSIINAIVEWQIASNGRFDKKSMPLIEELLVKAHKQDISHTSALWSIIASLYIKRLVHHKNFRKYKSANTDTTNLDLAKLIFLKSDKYNRICDYNPFAHSSPQNIILKLNNKNRIIILKNIALNAIKGHRKDIFLWCCAEIYHSGVTTEELLLDWHKTFNYKFRSMRPTSQKDMENELTNNGLKFLNNALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7R2</accession>
    <name>A0A8J2T7R2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ZYBA0S04-10660g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_10660g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7R2"/>
    <dbReference type="OrthoDB" id="10260443at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006797">
        <property type="term" value="P:polyphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01115">
        <property type="entry name" value="SLC13_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14478">
        <property type="entry name" value="SPX_PHO87_PHO90_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF110">
        <property type="entry name" value="INORGANIC PHOSPHATE TRANSPORTER PHO87-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="453"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="492"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="548"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="590"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="630"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="674"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="718"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="746"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="770"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="813"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="853"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="898"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="1"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="66"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="245"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF89704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="928" mass="103589" checksum="8EAC18150564DC92" modified="2022-05-25" version="1">MRFSHFLQYNSVPEWQNHYMNYSELKNLIYTLQTDELRQNGELEGEENATASSSKLFGRKIKNKLFSKGSSSSSSGDKERDKSAQGKRESSVDKADKAPYETIQEETFELDELPSLKLNPENMTAQKHSPMRRFKDKFFDSRRSSLSSEGKTLFSPQDAFLTNLTAEKLKVDDFYKRTEAKFYEKFGKLVKDLEKEGVCQNFGTIFPEELSEEDVEFSNLPPEMDKTGFQEVELTEVPSTSISVGRRETRDTKDTKSDYYSEEDLEDDYDDLEAQDNTALLNCKQLDVESQRKSLLKQSIIYLYIDLCQLKSFIELNRLGFGKIAKKFDKVLHMNTREELIQSGIFFDDTYIFQPQTLTLLNSKIAHLIEFYAIIKGRPGDIEDCKRELKCYLHDHIVWERSNSWKDMLGLLSWEKELGDTAQSDYRSHLVLEYKKFNLPRPIRIGKFEFNQLWIPSMFLTMKALNISIIFIFTLILMVVHTFKDPAQGRCMALVECVALLWATESMPLFATAVLVPMLTVLFKVLKDSDTGHLMKAADASKEILGEMWSSTMMILLGGFTLAEALAQYDVAKVLASWLLSIAGVKPRNVLLMSMGVVFFLAMWISNVAAPTLTYSLMAPVLDSLDADVPFAKAIVLGVAIAADIGGMSSPISSPQNVVSMDYLKVYGIGWGQFFATALPCGVLAMIVCWGVICATFKIGTTKLEKFKPMKANFTLKQWYVIFVSLGTILLWCVEGKVEPAFGSSGQIAVLPMVLFFGTGMLSVRDFNTFPWSIVMLAMGGVALGKAVSSSGLLATVALALQKRIENDGTMAILCIFGILMLVVGTFVSHTVSAIIIVPLVQEVGSKLADPKAAPILVFGCSLLASSGLALPSSGFPNTIAISMEDRNGKRYLDVKTFITRGLAASLIVFVCIITLGYGIMKSILKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7Y1</accession>
    <name>A0A8J2T7Y1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">separase</fullName>
            <ecNumber evidence="2">3.4.22.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_03950g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>All bonds known to be hydrolyzed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by a hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage.</text>
            <dbReference type="EC" id="3.4.22.49"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.22.49" evidence="2"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10255632at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051307">
        <property type="term" value="P:meiotic chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005314">
        <property type="entry name" value="Peptidase_C50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030397">
        <property type="entry name" value="SEPARIN_core_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12792">
        <property type="entry name" value="EXTRA SPINDLE POLES 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12792:SF0">
        <property type="entry name" value="SEPARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03568">
        <property type="entry name" value="Separin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51700">
        <property type="entry name" value="SEPARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C50" evidence="6">
        <location>
            <begin position="1414"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF88876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1608" mass="186927" checksum="EC46F6A447402D47" modified="2022-05-25" version="1">MSKQEEPLNEMSLNTLPGKPNALLSQKKALPYKDNCTTVHNVENSLERLSELLKDKNSIAKHKTEISNYLSALYEEVKSTHTLGLLNRISKQHLVFVVNLFNNQDALDAGLETLHLYNETNSSPVKDLHEMLLADYTFCNNGYLFTLKILAMQAILKLKSYDEYERSLLQAFAHDERYLLKGSNFKIHTLIKLVLHFYSVLPNSRVLFGLKFLQYVECYNLEAGHYIKNMGIDMFEQQLLRYVPERAPVNYRMYLRCFYMQYSKSKPTPEKLMYAELLKSEDGPLKDENDLFNLVTTKDDEKLWGKFLSTGIQDLVVIATRTLQNEDLHYVLKSLIFINQILRNAKYEENRAKASLLDKGLLFVNSHLKKFEKYRRELLTLLQGFYDYCMENNEINRLTNVINTFYNCFVVLRDFEFLKYAAHLEVSRYYVSKDDKLVRPMLRKFEKFVSISSTFQQKLEVFIYIFNVHITMGDLSFSSLQQFCQDIFKSVFSKLKLRVYEEFKQCSEVMLAFLYSNIPSLPPKSKSWYPATRMLYSSLSGMFDLSAIEVNSDVSRWHMLYKYEVLIKTAYYFNLEMSRHSTLNLASITKRYIDKWVKRSLPLDEPLSPFEIEMIKMLLEYLKFNNFDKLIIDLINILKDKERYDCIKVEGEIYLCNALINLKMIEDIERTKHRVLGNACDLKVAKLEPLMTNLRAKMQIFVWEQDIANFEDLFIKYLPLVRSELFDVDNTNQMPVSKYVKVLLFNIELLESASKLHCWNNCIVNSVIEGKKALKLSVCLLKKISKLSQNSRLRLIRLLMVAYINLIETYIRLGISKDCEFYAKEFSKVICELGEPTVVFDCLHFLYEYYQLTGQRALAKTVLQKSNKTFDFIDGETNIWGLTLFLHDNGEYEKLNESLRIFFNADLSKTYLSNYWRLKRGYELKDSECLCKYKTQNDINKLNLMYQRIVNHLESDPFLKSIFESITAVPSCRPRQMSASCHLQTPYKFHLTANVNSSPRSSNMTPRKKHYKQKFDREAAISNLENLIKSAEEMKLTSLKNYEISQLAASYSLALFLLSSISPERINGIKVAEKMVLCDIPRSVPLYHDKILNNINSEIYGNFKLLPMDQSLDPIAIERKKLLNIQETFSKCMSSFNVITIDICPSNDSLLLSKIDSKFGKNVQLRMPLNRAYARDLDCHKLTFGEAKEELESIIQENNRSTSIEVTSAIKTKEERKAWWETRYRLDRRLESLLSSIEHYWFSSLKGLFSPITTKPDVLKIFTLKFYEILHQNLPSRKQCGNPSMFTQIDSWIIELLLQLNPQDDQFALMMEDLLYFILDILLFQGEENAYDEIDFGGLHFHLEDQIRKFREQYCEEEKVVHTFLVVGTGCHAIPWESLSFMKSISVSRIPSYSSLSDLLTRFDYNLFPEISLRNKISMILNPQGDLNRTESRFLAQFTNIMQSKMGSKLLVNRKPDEIGFLEMLKSSNVFIYMGHGGGEQFVRLKEIKKCDRIAPSFLLGCSSGSMKIFPNLEPTGTVYSYLIGGCPLVLGNLWDVTDKDIDKFSESIFNKIGLYRWGNEMGQEPSSIGEKINICSAVSSSRDVCHLKYLNGAAPVVYGLPMTFINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T855</accession>
    <name>A0A8J2T855_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Elongation factor 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Eukaryotic elongation factor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN860_01024g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T855"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="UniPathway" id="UPA00345"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002182">
        <property type="term" value="P:cytoplasmic translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18626">
        <property type="entry name" value="CD_eEF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1390.20:FF:000001">
        <property type="entry name" value="Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.990:FF:000001">
        <property type="entry name" value="Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000193">
        <property type="entry name" value="Probable Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
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    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
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    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
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    <dbReference type="InterPro" id="IPR015688">
        <property type="entry name" value="eEF3_ABC2_chromodomain-like"/>
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    <dbReference type="InterPro" id="IPR047038">
        <property type="entry name" value="eEF3_chromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040533">
        <property type="entry name" value="EF3_4HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047036">
        <property type="entry name" value="EF3_4HB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF5">
        <property type="entry name" value="ELONGATION FACTOR 3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17947">
        <property type="entry name" value="4HB"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00005">
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        <property type="match status" value="3"/>
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    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <feature type="repeat" description="HEAT" evidence="15">
        <location>
            <begin position="171"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="17">
        <location>
            <begin position="426"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="17">
        <location>
            <begin position="667"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="971"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1009"/>
            <end position="1031"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004815"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CDF90121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1045" mass="115663" checksum="AA8DE65086CE71F8" modified="2022-05-25" version="1">MSDSQQSVEVLNQLLARLSVATPDKRDEVANEVSSFVNGNIIEHDAPEHFYNGLFKAIKDKKSAANALETVAHIASDSDLSPSVEPFIVSALPAICEQTGSKDKEVQATATKALQAVVTAVNPVAVKAVLPHLTKSLSETNKWQEKVAVLAAISTMVDVAKTQVALRMPELIPVLSEAMWDTKKEVKNAATATITKATETVDNKDIERFIPKLIECIANPREVPETVHLLGATTFVAEVTPATLSIMSPLLSRGLAERDTPIKRKSAVIIDNMCKLVEDPQIVAPFLDKLLPGLKTNFTTIADPEAREVTLRGLKTLRRVGAVGEGDVLPEISHAGDPATTLGVLNELLKGEKVAKKFKPVIEHIACIAGDLIDERIIDQLTWFTHVLPYMTIFLHQKQAKDILDEFRKRAVDNIPQPPKFDDEEEEGEDLCNCEFSLAYGAKILLNRTQLRLKRGRRYGLCGPNGAGKSTLMRAIANGQVDGFPSQEECRTVYVEHDIDGTHADTSVLEFVFQGDVGTKEAITAKLKEFGFSDEMIAMPISALSGGWKMKLALARAVLKNADILLLDEPTNHLDTVNVAWLTNYLNNCGITSIVVSHDSGFLDNICQYIIHYEGLKLRKYKGNLSALVARVPSAKSYYELGASDLEFRFPEPGYLEGVKTKQKAIVKVSNMSFQYPGTAKPQISDISFQCSLSSRIAVIGPNGAGKSTLINVLTGELLPTTGEVYTHENIRIAYIKQHAFAHIESHLDKTPSEYIQWRFQTGEDRETMDRANRQINESDEQAMNKIFKIDGTPRRIAGIHSRRKFKNTYEYECSFLLGENIGMKSERWVPMMSVDNAWLPRGELAESHSKMVAEVDMKEALASGQFRALTRKEIEEHCSMLGLDSELVSHSRIRGLSGGQKVKLVLAAGTWQRPHLIVLDEPTNYLDRDSLGALSKALKVFEGGVIIITHSAEFTKNLTEEVWAVRDGKMTPSGHNWVTGQGSGPRLEKKEDEEDKFDAMGNKISGATKKKKLSSAELRKKKKERMKKKKELGDAYVSEDDEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T875</accession>
    <name>A0A8J2T875_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ZYBA0S07-03136g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_03136g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat WDR59 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T875"/>
    <dbReference type="OrthoDB" id="311712at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16488">
        <property type="entry name" value="mRING-H2-C3H3C2_Mio-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049567">
        <property type="entry name" value="WDR59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049566">
        <property type="entry name" value="WDR59_RTC1-like_RING_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17120">
        <property type="entry name" value="zf-RING_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="110"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="197"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="RWD" evidence="8">
        <location>
            <begin position="432"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="570"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="668"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="598"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="709"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF90530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1142" mass="128835" checksum="9625D56569B69F54" modified="2022-05-25" version="1">MAGYVGGDPYQSPTFGKSLSLKVEAGVNALSINPSGRDVVLASKQGLYIIDLDDPFSPPRWLRHITPWQVADVQWSPHPSKSYWVVSTSNQKAIIWNLAKPSSNAIEHVLHKHYRAITDINFSPQHPDILATCSIDTYVHAWDMRSPQRPFYTTSGWLSGASQVKWNFNDQNVLASSHGNDVCIWDLRKGSTPLGNLVGHGSSVNSVDFNRFKQTELMTGSNDGTVKFWNYSLSCNEPIQTIRTNFPIWRGRYLPFGEGCCLMPSVGGSNSVYVASLANKDIEETKDTKLQPIYAFKGHSGRVTDFVWRSRSHYNDNESDREFQLVTWSEDCDLRLWPVPESVYGKVNFDRKKRPQEHLPSYDYISYNKDPENEHTQTENNYRRIHETFVTTSGVKAKKNVNHISWLSGVRMNHNNSPESIFKEKTLQNLGEEVSSLGHKFPKVVFEKISVSTGELIITLNGPWSETNPEEYVFLRIDVKVPSEYPSKGNPPRFTIEDNGNLSNQQRQEITQNLKQISEKCTNSGLFCLEPCLRLLLGEKVNLDDIGQDNEPLLNFDIAGQFDLENISSPASAQEDTAEYLSDSTTESEENRAGDVFSTGQKMDGQSQMDLTLDSTPVPTECGAVWSPGGYLLCFFASESKQDKKQQTMLRLASRDALKYQISGLKKGSSKNAGIQEDSTADAGKGKRPKRYVDTIPTSASAVRSTYDTSDEESPSDEYSDSFEDDWGDILANDIVVRTKLPALRGRVSKTFESVHSGSGKTTESKKDKNVLIYKDFSHLIPDRKDLALEYRFTDAPQEELARHNALVAEKYGLEEKSHCWQILSDLLMTNEETDPYNLIWDNHPMGISWLIKEALNYFEKRNDLQMLAMLGAVAASTEMSALCYTRTQNEDNEDKKLESTISFNPNESAYSWRTDMFSHLSSSNGFAPSLLEGNRHKHSPDEISIKSEDYFSPKGNSHGIQGPNISPPVSNTAVSATVAHNQQISSRMPHVTIELTNDDVLSAIKEPVRRLLDPDDAAKFRFYSYQYSKLLYRWGLAMQRVELLKIGFESTRQDQFNFDSSIPVDMQGLFGDIRLTWKEDKNKSATFRNCNYCALKVTRNVFVCESCEHIMHASCARDWWTQSKECASGCGCPCPELFKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8E2</accession>
    <name>A0A8J2T8E2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S05-07074g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_07074g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5588096at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005826">
        <property type="term" value="C:actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902716">
        <property type="term" value="C:cell cortex of growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007121">
        <property type="term" value="P:bipolar cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036267">
        <property type="term" value="P:invasive filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022018">
        <property type="entry name" value="GIT1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013724">
        <property type="entry name" value="GIT_SHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039892">
        <property type="entry name" value="Spa2/Sph1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21601:SF0">
        <property type="entry name" value="PROTEIN SPA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21601">
        <property type="entry name" value="SPA2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12205">
        <property type="entry name" value="GIT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08518">
        <property type="entry name" value="GIT_SHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00555">
        <property type="entry name" value="GIT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="GIT Spa2 homology (SHD)" evidence="4">
        <location>
            <begin position="45"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="GIT Spa2 homology (SHD)" evidence="4">
        <location>
            <begin position="95"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="125"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="579"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="670"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1233"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1426"/>
            <end position="1591"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="299"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="157"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="237"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="595"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="670"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="689"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="778"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="800"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="861"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="883"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="907"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="967"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="990"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1049"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1075"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1086"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1115"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1133"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1234"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1271"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1321"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1354"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1364"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1509"/>
            <end position="1536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF90021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1758" mass="192351" checksum="C765ADEFD031E2A5" modified="2022-05-25" version="1">MMDGGADVSTVHHRDVHRYFTALQKYFNDAETKHDRSNSPRAQKARAKLLKLSASQFYELSTDVYDELQRRINEDQSQPDYLLPRATFHMKRNQARQKLANLSQTRFNDLVDDILFEIKRRGYNVSPNADASDRGSGGKAASNKHSNSEKGKENHNSRNTKSSVSDGSLSQIPPTATIQTSKVIPQKASIDWSSEEEDNEDAANSGAKNKQAELRKTDAPGANKGDLKSNKKPNLPHINSFQQPNTTPLLSASESCHYGDLIDSPVLTETGNPLLEDSPSDDFNKVKDLNAEKSVNDKQENASAELPTDQTQLDQLNEEIAALKRENASLKQNRSLGGFTSNENFERDLNSLSAELGTLSVENEQLREKVSELEFKTKNMVPKQLMPLKTKHEDLDNLNIKPLFDRNSMAKYAAHDGSIPFESVEEFHTLVQKLFESIQSRDDELGRNLFENISRLSHTVSQLLLLVDVPEFKDEVLLLRASLSHTITAIRYYAVYGALLPKITVQAAISELAFAFCNLVRSCKIKGNEQMILNFSRKTAVLDGTKDQITPQTPEDSAPSMFFKSSSLHNPSEIMKFSDTTQDDVSPVKPLKITQKASVSPSGRTPTPRKISSGLLFGSMVSTKSTSSQSNGSGTGFSFDKTRELNAISPTSAHHDGSNVNLRLAKVESSDNIPTLSKSSRPEGGFSFEKTRGSPTKDGEPSKISSDKDDPDKTLNGETDTFKKSTVTSKSAAVDGPTVIATSPNAASPPLNKMEDSNEYLAGNADSALQKKFHDNIEISSGTAESKGTESSPLGSPGVEKTEKAKITKSKTDKSPDKSENNDTKSPASQSSKGEPKKGSKSKVSGSEALKSTVESPNQETKSKQGNEKVSNNHDRKPKQHNSKMDQKDVSEKHEIKSKQHNNTSKIGEDDVSEKHDHQESRSKKAPGRSFTDKLRTFANNAGIGLRVEKDLNKQVGGVETKGNGLKSDENKIAERKRSLTIDTGKSKKNNNSDVSPNSSSNKSIGGIKKSGTIPSFGAKESGIEKSHEKKHRSTDKEDVHTAKKLGNNRHSGNQSTENLKKQSSVTDKGAQNGKEIRKHSEQKKSAGTSKSPKNEQKSPSTVKLNGHTKKDEVNTSGRSDSSNKETSSGEVMPKEKAGKKDENSHNMNLGDLKANGTKDGVIPSVTLNDGKGETDITPKSKLDFVGAKKVNGVQEADDVPEKLPNFYFNNIPVPAPTNSLLTDKLKKSFADIPSEDETEDISDINNSPYMSDDGSSFHAFKQSWREENTGRSSPLAQQQSFHDENTTVRKMAGGLNITKETALRKDSDISSKVVTNGPRDLQKDFEGSEDHGTTGNGNGEVSSSWKGKEELPKSTTLQSLESVKTDSIAKKDSSRKTETKDSRESVNESRVDTVNKQKNSSKENGLASDLSADTGVKLNYNDFEFGNESGVTAGDRNPKTNSASMNDKSAFKGGNRLVASDKLYNKSRGYDPSGSPALPGTVEQRSHLNGKKTLKGEYTEDLNADPSFKQNGDASKTPTSDSVTATTPKLQSVKLTKTHTSKGDGFMQSPAIPKTPVVKSPALKTPASKGSTTRSPFRSPATRDEDFDDDREISFDVDAFDIANPDNTLSELLLYLEHQSLQVIATIQSLLTSIKEPHATKGNLRKESNAINQVIRQMVDATSVSMNQSRNASLKEHGSWVVKSLEDCYLRMTTLCQLKRDGEFVDKSGDDEFADKHFKQRLAGIAFDIAKCTKELVKTVEEASLKEEIAFLNARLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8E3</accession>
    <name>A0A8J2T8E3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Heat shock protein 104</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_06876g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T8E3"/>
    <dbReference type="OrthoDB" id="47330at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070013">
        <property type="term" value="C:intracellular organelle lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070370">
        <property type="term" value="P:cellular heat acclimation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19499">
        <property type="entry name" value="RecA-like_ClpB_Hsp104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1780.10:FF:000003">
        <property type="entry name" value="ATP-dependent chaperone ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000017">
        <property type="entry name" value="ATP-dependent chaperone ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000120">
        <property type="entry name" value="ATP-dependent chaperone ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000025">
        <property type="entry name" value="ATP-dependent Clp protease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000010">
        <property type="entry name" value="Chaperone clpB 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="entry name" value="Clp, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050130">
        <property type="entry name" value="ClpA_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638:SF18">
        <property type="entry name" value="HEAT SHOCK PROTEIN 104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="Double Clp-N motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Clp R" evidence="14">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="13">
        <location>
            <begin position="580"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="867"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="896"/>
            <end position="911"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF90013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="911" mass="101992" checksum="AB5737BC712895EC" modified="2022-05-25" version="1">MNDETQFTDRALTLLTLAQKLAQDHQHPQLQPIHLLAAFVDTPEDGSKPYLQNLIERGRYDYDNFKRTVNKHLVRVPAQHPAPTQITPSYSLGQVLQDAAKIQKQQKDSFIAQDHLLFALFNDSSIQQLFKEAGVDIEAIKQQALEMRGNQRIDSRGSDTSTPLEYLSKYALDMTEEARKGKLDPVIGREEEIRSTIRVLARRIKSNPCLIGEPGIGKTAIIEGVAQRIIDDDVPTILQGSKLFSLDLAALTAGAKYKGDYEERLKGVLKEVEESKILIVLFIDEIHMVMGNGKDDAANILKPALSRGQLKVIGATTNNEYRAIVEKDAAFERRFQTIEVREPTLRQTVAILRGLQPKYEIHHGVRILDSALVTASQLAKRYLPYRRLPDSALDLVDISCAGVAVARDSKPEELDSKERELQLLQVEIKALERDVEADSTTKERLKQARQKEASVQEELEPLRQRYDEERHGHQELTQAKRKLDELENKALDAERRYDTATAADLRYFAIPDLKKRIDQLEEEVAEEERRAGNSSMIQNVVDSDTIAETAARLTGIPVKKLTESENDKLIHMEQDLSSQVVGQMEAIKAVSNAVRLARSGLGNPRQPASFLFLGLSGSGKTELAKKVAGFLFNDEDMMIRVDCSELSEKHSVSKLLGTTAGYVGYEEGGFLTNLLQRKPYSVLLFDEVEKAHPDVLTVLLQMLDDGRITSGGGKTVDCSNCIVIMTSNLGAEIINAQPGSRIQESTKGQVMSVVGAHFRPEFLNRISSIVVFNKLSRKAIHKIVDIRLKEIQKRFEENDKHYKLDVSPEAKDFLARYGYSSDLGARPLNRLIQSEILNKLALRILKGEIRDKETVRVVFKKGAARGTGRVEDEEEGLEVLPNREAANKSSPATAEMDVDDEDEMADDDDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8F0</accession>
    <name>A0A8J2T8F0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="16">ZYBA0S05-07448g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_07448g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Cooperates for the ATP-dependent vacuolar transport of bilirubin and glutathione conjugates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000020">
        <property type="entry name" value="ABC metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000013">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002123">
        <property type="entry name" value="ATP-binding cassette bilirubin transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056227">
        <property type="entry name" value="TMD0_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24357">
        <property type="entry name" value="TMD0_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="179"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="432"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="957"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1011"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1085"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1110"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1199"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1224"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="300"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="610"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="971"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="1292"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="873"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="873"/>
            <end position="893"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF90036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1549" mass="174537" checksum="326555ED42157C66" modified="2022-05-25" version="1">MEVLSKNAKCQYGYRPYIDNKINALNPCFLSLVIFIGNLLFILVGLFQLWRLSLTKKIPANLSCRSWKSISMNHLVQLSNVLLQVTLIIVQLSIVYATRFEYTQVLKCSLWASLGFAALVSVPTQYLQYFKSNCAIGNQLFYYFSQTIILLFQVSQRIGHWPDERFNLVRGTLGGALELALLVNGIAILTYDLCFYKPAKKLQDYYKENDLFPTTNIFANLTYTWMNPLITETYKHGKIKDPRHMPIAPINLHVEELTGKLRAAWENETWIGRNSLFRALLKVFGRPIVIGVALETTRELLDAVKPQFLMFFILCFNVDSDSKYPPLHGLFIAVTLFFLTVLSTVLQNQYFIFIFEAGLGMKGSLISLIYQKSLRLSVAGREKHSTGDIMNLISVDARRVQMFFEDCQVIISSPLLVVVVLISLYWLLGKAAIAGLVTLAIMIPINSFLSKRVESLYKTQMKYKDTRIRTTTEILNSMKSIKLYAWEKPMLQRLNHVRNDLEVKNFAKIGVVENLISFAWNCVPLMVSCSTFVLFSFINSSPLSPEIVFPALSLFELLNEAIYSVPNAITNIIETKVSVGRLKKYLLCEELDDSFMHYKKDEAHSSLPTVEINNATFLWKSEKSIKGNGEHDEEANVETSLAALKNIDHFEAKKGALTCVVGRVGSGKSTLLRAVLGQLPCISGSQELVAPEVIVRAKTVAYCPQSPWVMNASIKDNILFGHRYDENYYNLTIKACQLIPDLKILADGDETLVGEKGISLSGGQKARLSLARAVYARADLYLMDDILSAVDAEVSKNIIDKVLDEDNGLLKNKTIILTTNAVSVLKHSNMIYALQGGQIVEEGTYEEAISRGENSSLQKLIKEFDTFAGKAENEDTRSIDEEVDKSKQKHDEESLADSVRSLEAAEAAEAAQMVNISSRRASMATFRAKPSVEINEFNESNSAKEEKSEHGKVKKEVYIFYIKSCGLLGVVLFFLFMVLGRLFELSENFWLKYWSESNQKNGSNNDVLKFVGIYALIGIASTSFDTLRIVIMLLYCSIRGSKKLHDTMARSILRSPMTFFETTPIGRIINRFSSDMGTVDDGLQFIFSFFFRSILNYCITVLLIGFNMPWFLAVNAVLLVVYLYYQAYYITLSRELKRLASVTFSPIMSLVSETLGGHMVITAFRHVSRFYFLSFENTQIHIDAQFHLRSTNRWLSVRLQTIGAFMVLTTALLTLATIGTKKQVTPGMVGLLMSYALQVTSSLMWIVRMSVMIETNIVSVERIYEYCQLPSEAPAIVESMRPDRSWPAKGNIVFKDYSTKYKPELDPVLKGISLNIKPREKIGVVGRTGAGKSTLSLALFRLLESTEGSIEIDGLNIAKIGLYDLRSHLSIIPQDAQAFQGTVRSNMDPFDQYPANEIWKAVELSHLKPHILKMFKDENPDKPQSNEIEALDVKISENGDNLSMGQRQLLCLSRALLNKSKVLVLDEATAAVDMETDQIIQETIRSEFQDRTILTIAHRIDTVLDSDKILVLDKGQVKEFDTPANLLSNKKSMFYSLCQKGGYLNKNGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8L2</accession>
    <name>A0A8J2T8L2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ZYBA0S08-02850g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_02850g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316461">
        <property type="protein sequence ID" value="CDF90781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T8L2"/>
    <dbReference type="OrthoDB" id="6718656at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030907">
        <property type="term" value="C:MBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033309">
        <property type="term" value="C:SBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000361">
        <property type="entry name" value="Swi4p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.10:FF:000006">
        <property type="entry name" value="Swi4p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018004">
        <property type="entry name" value="KilA/APSES_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051642">
        <property type="entry name" value="SWI6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828">
        <property type="entry name" value="ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828:SF7">
        <property type="entry name" value="REGULATORY PROTEIN SWI4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04383">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01252">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="5">
        <location>
            <begin position="44"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="557"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="8"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="150"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="343"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="756"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1013"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="22"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="359"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="760"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF90781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1098" mass="121058" checksum="33AC73A078E903CD" modified="2022-05-25" version="1">MVYGAVMMDSFKGNQPDDEPSKNSTGGATDFSTTTMTPSPRPVIEVATYADTDVYECYIRGFESRIVMRRTQDDWVNITQVFKIAQFSKTQRTKVLEKESNDMRHEKVQGGYGRFQGTWIPLEDAKYMVTKYNIRDPVVTTILAFQLNPNNPPMKRSKNSVLKRTSPDGRIASPSSYNKTPKKNKVGGSTTRKTKKNNMLLHANPSPLQNLVFQTPQQSHMPSQLATNAATTVSATSQGNQLSNTKGNTVPHINTEETPLALGYSATQKPLQFYPVPTSLNHPHKSRDFAQSGNESFDASADIDKANSQSFLTFIPEGPNTGVFSSSAHQQQSQQRVPSILVNGNGSFKKASGKRKKTLKTDENSIEMVSHDDDALGGAPPPPPIPGATPGSSMSAGKQLKHKLKGRTGLTAKRANNAQDKAQQNFKMMQKQLMWQNIRQQHKNTPNIAVPFKMSGNQPNSNNSSMDMFSTGENPTPMSSRSSTPNTFAGLPTAPATAHGNEHADTIGPPALSVEEYKEMILQVLSSEDGCDKDYQLPPQLYHPPLNFDVNFEIDDQGHTPLHWAAAMANVPLIKLLIALNANALQCNLRGFNCITKSAFYNNCYKANTFSEMVSILRICLITPDSNGRLPLHYLVELSVNKSKDPVVINFYMDTIIHNLGQEDYTLLRMCLNFQDSMGNTVLHLAALNLNLELCNKLCYLGSSMDITNCDNETPASILAKFNLVPPTNTNTHSQLYRSAPTPQGFSTAINHVGPVGTTPRPTSHPSLLSGENSNRDGHSHKLQHEVMSSIEENEKHQNQVKLTPSVNSKKTDVRNFLPGSDSTSLSNLMDDLSNMNSFVTSSVVRDIKTTPSKLLECSPIMRKKKPTTVVTSSNLKKSALPEAIQSPLVPVIASPGPDSNEKEMSSVAKLASKLGKISNALKVSINAQVSSVTREIESASEGINCIKQNVFNITRHEKDLLLQFNDGQGVESVEQMEAGRNQMREYVHDGRQMFVQCLEKSQALNLATLVQEEESRVDPDPGSSPSKEGSNDISLKLAAELALLQFKRRLRIQRVTDARTKIDSNNKISKYRRLIGMTIENIDTKLDDIEVYLKTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T944</accession>
    <name>A0A8J2T944_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_02630g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T944"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02659">
        <property type="entry name" value="peptidase_C19C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000044">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000016">
        <property type="entry name" value="Ubiquitin-specific protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0645">Protease</keyword>
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    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
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            <end position="177"/>
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            <end position="527"/>
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        <source>
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    <sequence length="1184" mass="137934" checksum="8EF41CE3797E8456" modified="2022-05-25" version="1">MSDEVAGHVEEAIDLGKTADDLLPSLSEDLETEPQVEGSFTWRIEDWSKLTADKYVSPRYKIGDFEWDILLFPQGNHSKSLAVYIEPHAKGKKNEQTGEMEYEDPSWSCCAQFTIVLSRPGEDKKIQIVNSSHHRFNAIDTDWGFASFIDLNHLKYSSKSRVSGLLNHGKLNITAFVRILKDPTGVLWHNFINYDSKKVTGYVGFRNQGATCYLNSLLQSYFFTKFFRKLVYQIPTSNENPNDSVVLALQRAFYQLQVSSFPLDTMELTRSFGWDNAEAFTQHDVQELNRILMDRLETRMKGTAVENKLNEVFVGKMKSYIKCINVDYESSRIEDFWDVQMNVKNLKGLQDSFENYIEVELMDGENQYAAQDYGLQDAKKGVVFESFPSVLHLQLKRFEYDFNYDQLVKINDKYEFPERIDLSPYMDKDILKTNPGPRWYELHGVLVHTGDISTGHYYALIKPGIEDQWYRFDDERVWKATKKQVFDENFGFDRLPDDKIRTMTREQYQEYFVARHTSAYMLVYIQEDKKDEILQPVTSADVPEHVVMSVDRENKERELREKEIKEAHLYTNLKISSMSNFVHYQGFETFPNNHSSLFSPDLNPDNSMPTSLRIPSKSYVRDLFKEIKNSLSIPLDRNVRYWKMEYRRNGTIRLDEPFPMSLEDQTLETALQDDAARKISTLNIFVEEPYLDLGFLLKLKKAGLINNSEVTNDLIHTLRQDITALIPESEIPQTIEDSNRQLLFIKKFDPLKQTLTGAAYCSVSQLDEVSALSKMVAQFIETDKQIDFYEELQPGTIEPVSLKERFYIAELCSGDILSFQVPDTQAADIFPVYKTMTDFYQYLRHRIKLKFIKAQESTEEYVLKNDSLDNFEFWISAHASYSDISRVVSQYTQVQPEYLKIFAVYANGKFPMKSDSSLSDYILKDFNCELTPSFEYEVLSMSLKELEQLKSMKFYWLKNSYIHYQSYEFKVTNNCTVSEFLDKIQAKIGFSDEDKSNILLWTNYNFHFNGVLAHNSTLKSIGKSVFLFGRILPEELALVKQLDGMTEEEDASIGDEEDMEIIKTLKPKIEGCLVMVVQYFKDPENRHGISFLFNLIPGETFLKTRARLHAKFGLGQKEFSKIKLRVWYTTPHGHSFRSLQDYTDEELDKLILYNIMSNLDCIYMDHPDRLRSQNSHDRPMFIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T997</accession>
    <name>A0A8J2T997_ZYGB2</name>
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        <submittedName>
            <fullName evidence="8">ZYBA0S08-02960g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_02960g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CDF90785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
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        <property type="term" value="P:endosome organization"/>
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        <property type="term" value="P:receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAM_BOI-like_fungal"/>
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        <property type="entry name" value="Transcription Factor, Ets-1"/>
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        <property type="entry name" value="Boi1/Boi2-like"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="35"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="7">
        <location>
            <begin position="258"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="842"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
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        <location>
            <begin position="143"/>
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        </location>
    </feature>
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        <location>
            <begin position="173"/>
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        </location>
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        </location>
    </feature>
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        <location>
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            <end position="419"/>
        </location>
    </feature>
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        <location>
            <begin position="439"/>
            <end position="496"/>
        </location>
    </feature>
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        <location>
            <begin position="510"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="556"/>
            <end position="575"/>
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    </feature>
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        <location>
            <begin position="636"/>
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        </location>
    </feature>
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        <location>
            <begin position="1018"/>
            <end position="1048"/>
        </location>
    </feature>
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        <location>
            <begin position="1066"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
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        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="403"/>
        </location>
    </feature>
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        <location>
            <begin position="446"/>
            <end position="459"/>
        </location>
    </feature>
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        </location>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
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        </source>
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            <dbReference type="EMBL" id="CDF90785.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="1101" mass="119522" checksum="198A5BB964F2030D" modified="2022-05-25" version="1">MSFKKLQPSLTKLDTLDSGSPADGPGSAMSSGSTRRSAIYIVVNEYSKRMQDELDMKPGDKIQVIMDDEEYNDGWYYGRNLRTNEEGLYPVIFTQEMTFTSKPGLMRAKSAKRVGSLGKGGSNASFNGSSSELSTPQVIETAAPVTVQQKKPVDRQVSVKSTMSDIDRALEELRGSSLEEQTSPGPAAGYSQRQRQSPAKASQVKRGSMVFTTKHLDVGKKAESSALPRSVSKDTSAATSTINGIETSELIPSMAKGWTPQQVTAYFISSGFDVQSASRFQQHKISGAILLELELAHLKELEINSFGTRFEIYKEIEALRELVNSPTNLNSQRPVTQNNEETATASTDKDNSKSLTAPVFFNHGPTYRGHVKKASQSLDDIDNEFIQQAQGKEQHRTQEHEKNTQTAKHKPTSLGNTFSQAVPSSPIVDGDLANLELFSSPRRAPKPPSYPSPVAPPKSPVGNLTREFIAHSGSGYSPPTIYEKNAPSVESNYSDTNEKVADSLNVAPEDLNAAPSSFNATSETLQKETSKYKFPPESSPNPSSTNSGIGLGLAVGTPVSVNSPRSPLSPRLIDPWSNEQSAANQNTQQPAINHQIAASRQSATTRSSGLVPEAAHKNSLSGASFMDLINKVSHKNPGDEYVDEEQTSNYAEPVGRPTSSVYNTHSRATSTVQAPSSHYTDFQDLRRASSARSYFSAKDDHEDSSTPVNSGSGAFATKAKDSSDPAKGSSEDKSQLTPVEIRTNSKSAVNTPLAPTALSPSKPKSATKTKFKRRSVSAKEATPNGKQKSSAPDDDKKKRSVSEAVKGTSMLGKNHKGSSKKRQTSAFMEGIRNISVEKAMADADCSGWMSKKGSGTMGTWKTRFFTLHGTRLSYFSSTSDTRERGLIDITAHCVVPAKEDDKLVSLYAASTGKGRYCFKLIPPHPGSKKGLTFTQPRVHYFAVDGKDDMRAWMAALIKTTIDIDTSVPVISSCSTTTVSLSRAQEMLSQAREETRLREQQYMNEEDEDQLLWEREQQGNKENKENKENSEEYTLSATTQPSSSGSGTMATTGFASPYLLASGVLSPNLHRSGTAKSGKFQDRTPEKLKQQDAHLGVGTERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9A9</accession>
    <name>A0A8J2T9A9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S08-03356g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_03356g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316461">
        <property type="protein sequence ID" value="CDF90801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T9A9"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01366">
        <property type="entry name" value="KISc_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000106">
        <property type="entry name" value="Kinesin-like protein KAR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972:SF28">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="4">
        <location>
            <begin position="362"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="81"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="279"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="449"/>
            <end position="456"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF90801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="719" mass="82713" checksum="9F2DE1DD6D89BA41" modified="2022-05-25" version="1">MNRLPTTPTKGGGSESSIPSPKFNGLSPRVYKQRNASTPSIPNSPVEHDKFVSNIATASNGAITKSSLHLTSFYKENIRELNQLQETLFQKKANMDILLDELSDYNSKYKNLEFKWETLKEEKTMKHQQANLKVNELAKFKEELATKKKFLEQGHQLHLQQVRAKNDADINKMDSEYRVQMERLKFAKIKKYQNERDSLLDKVEDVRNRITTNHTALENMLRECETNHYKVKEQWLQDYQMRWKENVRLNENFSNDIAALSQQIKNSLEPQLAERKHRLVLLREKSKKLKEDLEIARKESKDLKEMTKKTREETVQSKHKRQELQDYIVNTKSELQQIDQILIKEETMRRALHNELQELRGNIRVFCRIRPPLKNENPSMAHIKVNKFDDNSGSQTMEIVKNGLNSMPQHFKFDRIFVQNESNVEVFEEIGQLVQSSLDGYNVCIFAYGQTGSGKTYTMLNPGDGIIPATISHIFNWIKNLKERGWHYEVSCQFIEIYNENIADLLRSNNENTASNLKHEIRHNQENNTTTITNVTLCPLNSVEEVDALLKRANKLRSTASTSANERSSRSHSVFIISLKGFNHLTDEHSEGLLNLVDLAGSERINSSQVLGERLRETQNINKSLSCLGDVVHALGSNDAAKRHIPFRNSKLTYLLQYSLTGNSKTLMFVNISACVNHLNETVNSLRFASKVNSTKMIARESNAPRSENAGTASIYLQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9K6</accession>
    <name>A0A8J2T9K6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S05-04764g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_04764g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF89919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T9K6"/>
    <dbReference type="OrthoDB" id="760868at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF28">
        <property type="entry name" value="IMPORTIN BETA SMX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="4">
        <location>
            <begin position="25"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF89919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="945" mass="108483" checksum="50F19E475876868C" modified="2022-05-25" version="1">MQDEQAILACIEQTMVADAKVIKIAEQQLFQFQKQPGFTTFLLQVVSNQDVPLNIRLSSAIYFKNKIQRSWNAVNREDGIKQDEQQVIKDNLVQTLVSNCGDNHIRPHLTESVRGILNNNDHWDLTNIMNELLRSDKQEYIYTGLLLLFEVCIAHRWDMADGREEIDAVIFAVFPTVEAIASELVNREDYKSNELLYLILKSFKYSCLNNFPQYFKSLEKLNAWIQLHLFVCAKPLPKEVLELDTADRSLDKRVKVNKWGFGNLNRFIHKYSRVTKFVTEEFVSYVFNNIIPPILQEYFKIIQLWSNGSLWLGAASLHYLIQFLEKCCVLDEVYPLMEPNVETIIENVIFPCLCANPESVELLQDDQEEYTRRYFDMNKEGTTADVASSDFVFVVGHKRPEKLQTILPFINSVFLSFADNAQDVNCAYKQEGAMRMISTLFTFLEDPSDLEGIFSHFITLFLSQPQYPFLVARALETISIYGDGFKDMSTLSKLFELTYTHFMTSDVLPIQIEAADALKTLVVSNPNIHPHIAAQVPGIMEKLLGLSKEFQIDILSEVMEAFVERFADELTIFAEDLARNLVEQFLELGRSLLDSSSSAYSTGDQDQEIQASALLQTMTTMVMSMNKVSLVDQFMPVVKFVIINAQISFLTEIVDLMDSLALSSKTLYHQFTPTIWEMFHDVLDSFQTYALDYFEGYMTFFETVVTHGFPQDQTFLPPFLEILSIKLESDVDYDVESVMEILVFYALSMRDIPLFDKALRASSNDELELDPSSIVKLFLANLFVKPLETLQVCEREGATLTVLTQWFSCKFSSVFSIKLQILAIISLLNQPELPGSVKGFMPQFADKLVSLTEKLPEAIRKRDAMAKGEEGLEEMFSTEDPEDDMYFEEYEEDLKETVLDQVNAFQEMHNFFNQLDSQRSQEIVNSLSEDKKHSLKVILEFVSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAG3</accession>
    <name>A0A8J2TAG3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sulfite reductase [NADPH] subunit beta</fullName>
            <ecNumber evidence="6">1.8.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">BN860_04192g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the reduction of sulfite to sulfide, one of several activities required for the biosynthesis of L-cysteine from sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>hydrogen sulfide + 3 NADP(+) + 3 H2O = sulfite + 3 NADPH + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>siroheme</name>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Alpha(2)-beta(2). The alpha component is a flavoprotein, the beta component is a hemoprotein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.2" evidence="6"/>
    <dbReference type="EMBL" id="HG316463">
        <property type="protein sequence ID" value="CDF91299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1688044at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009337">
        <property type="term" value="C:sulfite reductase complex (NADPH)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050311">
        <property type="term" value="F:sulfite reductase (ferredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004783">
        <property type="term" value="F:sulfite reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.413.10:FF:000003">
        <property type="entry name" value="Sulfite reductase [NADPH] hemoprotein beta-component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.413.10:FF:000004">
        <property type="entry name" value="Sulfite reductase [NADPH] hemoprotein beta-component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000051">
        <property type="entry name" value="Sulfite reductase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000016">
        <property type="entry name" value="Sulfite reductase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="entry name" value="Sulfite Reductase Hemoprotein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.480.10">
        <property type="entry name" value="Sulfite Reductase Hemoprotein,Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045169">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010029">
        <property type="entry name" value="PRK13504.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493:SF47">
        <property type="entry name" value="SULFITE REDUCTASE [NADPH] SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493">
        <property type="entry name" value="SULFITE REDUCTASE [NADPH] SUBUNIT BETA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00397">
        <property type="entry name" value="SIROHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="Nitrite and sulphite reductase 4Fe-4S domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="Nitrite/Sulfite reductase N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00365">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0349">Heme</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="19">
        <location>
            <begin position="659"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00057613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00067595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CDF91299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1419" mass="159544" checksum="978B216B04E35948" modified="2022-05-25" version="1">MTITGACKLPEALLRCAQAVDSYRIFYTTPTKDTNSEYAHLPQAVQLLSHADPLSEVVEQLSTDSLTTLITDETTLVKSLPHVNSLQGFPVVVNVDLSLQDYSAIASFKDLSIPILISSNPETLTQNANRALEVATKSVRPVLHFINFANIRQSIDIDVKPTTIAEDPVDLDSLELEHNFELVSQGTPLTAIVDFSAYEFGELLPLHTCLVKVNVYRPWNFNKLLQLLPSSVKKIVVVQSANQKSNQNGFEPFLLDFFPDFDQLVERGIDQIIISNIGIVKDVGAALEEIVQNVNSAEPDLTLFIGDSAKDEDAVNELSACLKNVLNLENAYMKVVRQLFSPNLHILNEYTSETVQQNNPEYGYGLFLNQDRRRQNLISLARRSLDPSLYSTDGVQFVQLLGKWLAYHDQSLDENSLKEANVVAHEIFEYLEKNQVYKKSEEFLQVGATEDAYRFKSTWLIGSDAWSYDLGNSGVHQVLSSKKNINVLLIDSEPYQHKSQSHQRKKDVGLYAMNFGHVYVASVAVYSSYSQLLTATLEAAKFDGPSIVLAYLPYHSENDTPLQILKETKVAVESGYWPLYRYDPSKPDEDAFALDSSVIKRELQNFLDRENRLTLLSKKYPDFSRNLAQSASDNIAKKQERRAKQAYDQLLEGLSGPPLHVYYASDGGNAASLAKRLSNRAAARGLKSTVMSMDDVVLEDLPGEENVIFVTSTAGQGEFPQDGKALWDALKASTDLDLNALNFSVFGLGDSLYWPRKEDKHYYNRPSRELFKKLEVSAAKPLVPLGQGDDQDADGYQTAYGPWEESLWEALGVAGATVPEEPKPWTNEDMKINSDFLRGTIVEGLADDTTGAIHAYDQQLTKFHGIYMQDDRDVRDVRKAQGMEPYYAFMARIRLPGGKATPEQWLVLDRLSNETGNGTIKLTTRATFQLHGVVKRNLKHTIRAMNSTLMDTLAACGDVNRNVMLSTIPHNAKVHQQISDVGYKISEYFLPNTTAYHEIWLSGIDDRDYDPDWPSIFENRKEGPTKKKTKVSGDALVDVEPLYGPTYLPRKFKVNITVPPYNDVDVWSSDVGLVSIIDAETQELQGFNMYVGGGMGTTHNNKKTYPRTGSLFGYLPTEDIIKAIEGVMIVQRDNGDRKDRKHARLKYTLDDMGVEVFKAKVEEYMGKKFQAGRPFEIKTNHDYFGWVKDETGLNHFTAFIENGRVEDTVELPQKTGMKRIAQLMQRYGSGHFRLTGNQHAVISGIKDEHLDDFKKVLHEYKLDNTAFSALRLHSASCVGLPTCGLAMAESERYLPELITKLENILEGYGLRHDSVVMRMTGCPNGCARPWLGEVALIGKAPLTYNLMLGGGYYGQRINKLYRASIKEDEVIEILKELFKRWSQEREEGEHFGDFLIREGVIKPTLEGKYFHEDIPEEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WUK3</accession>
    <name>A0A8J2WUK3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_07140g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat ELP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WUK3"/>
    <dbReference type="OrthoDB" id="27911at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000400">
        <property type="entry name" value="Elongator acetyltransferase complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037289">
        <property type="entry name" value="Elp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44111">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44111:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="53"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="190"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="269"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="373"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="601"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="648"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="498"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="500"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF87472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="783" mass="88799" checksum="60E00E20AFB21084" modified="2022-05-25" version="1">MSNMILEATFIGANRQNQVSDVNKTATVAFGAGKTIALWDPICEIPRGVYATLKGHNSEVTCVKFIPNTDFMVSASEDHHVKIWEFSSLECVQTIEHYRHSIVSLAVWEKMIVVGSADGLISLWVLEDGQFILNYEFSICKGFLPLSLSVINIQDDGFLLAVGGTKTKVFIYSFVLSGTRIENFQSAAELEGHEDWIKSLAFRYKDSPGECLLATGSQDRYIRLWKVRLNELIDDSDENVRKPRLLSNKQYKFKIKSELRVSINFEALIVGHDDWISSLQWHENRLQLLASTADTSLMVWEPDESSGVWICGSRLGEISSKGASTATGSSGGFWSCLWFVHEDQDYILTNGKSGSWRVWSSTDHYVWEQRLGITGATKQVTDVAWSPRGDYLLSTSSDQTTRLYAPWQFNANGETRSQVTWHEFSRPQIHGYDMVCVESISSSRFVSGAEEKVLRSFDLPKGVSEILQRLSGCKFELANDLPVAASLPALGLSNKATSVEDNEIEEQEQEQDPNLRETNETRNISYEMTSSLTTPPFEDQLQRHLLWPEIEKLYGHGYEISCVDVSPDSKLIASACRSNNAQHAGIRIFDTSNWLERRPTLLFHTLTITRLKFSDDNKYLLSVSRDRQWGIWERDFENQTFNLIHKNAKAHTRIIWDCDWAPSEYGQTFFTASRDKTLKAWSYDAIPSTFVVENTVKFTLPVTAISVYKKVHNDKLIVAVGLEDGSMYMYKYSPAGFELMEQLNGNITPADKITRMRCSHSQRNEKLYLAVSSCDNSTRIYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WUM9</accession>
    <name>A0A8J2WUM9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_07888g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_07888g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4068315at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035361">
        <property type="term" value="C:Cul8-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019021">
        <property type="entry name" value="Mms22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28122">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SUBSTRATE RECEPTOR MMS22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28122:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SUBSTRATE RECEPTOR MMS22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1355" mass="157215" checksum="7435C55BE1A71BAE" modified="2022-05-25" version="1">MLVDETSWIVVPDSEEEGTLLFNPHDAENTSTSPEPGVLSVARGDWGMNKDVAISEGYQRSLRKRKAIQRMPYSLDRIKHRQLLEGYDMSGFESISEELKLPSPPRCVIEGTTKSCRHDVNEYLEAERDDESDRTSTSEEDIVKFRGKVVNMKTGFRGVLPRVAWEKALRQRDSTVNTPKRRRPSTNHKGIAKKKTTTRGKTINQDDALLGDLIVSEEEDQEAHIQLHEKPGEDFLQEMELINEHYHEKYDNQYLSENETSNERWTNYVFKLDQKDLEGISINAENDMAYSDEYSDFDSDLLEIGALENDRNAIDTMLSKGKQESRNMSSSLRGANVANGTSNVNKCATKSKIIHQRRCIRNCRKEKQGHLPASHQPPSKLTRNYAQTAAIKTRRQRERSKDFKSKKPEWPSMKIEPFKNTRRANTFSTVIEAPSNKYVVPKHSLSRTDNSESIECITDEDEYCHKISLDALEALTSGKNVKPPDTIVINLSNKHYTLSKFLQNETPLTLVKIFDHIIENGITDQELLQVSESLTLFLLHLNHLGISEVISDFHKKFRSKVNSLREKAKPIHFYQIAVCQLMLLEIAHYSDISSFAMMEIKSNIVNHVVSFFKLLAFCYEAVADCDMAYLYDGCDILSKVVLVLNMKNELWEKLREHTFQAEVCLLLVDTFPIEHSEWEILKLDQHYIALTQAFAFIEYCKKKLHWKYENHLLFPFDKIFKKRRYEDFNEEELSTAKNRVLGPRQVLARTTMFDKYLGLLQDTPLTNSVIERITPIGELSLNDSPAALVNRLNLLIILGRNTNINLEKKMENLIRPILTLQYLSQLSENSFKKVSHSMLGGILSLANILRLKNVLFKGKILIAIFKNLVFKNPNMLLQPWTDFLAQFIPIIEGSPKSIPLFLKEFYVCFSLMSQKELFANNVLPILQLYLKNLHMLGASWVQENLLQIIKGKIHLSTNWIDYYCTIGNFLIQEDAFSWWSFYMYNDLTDSLMNKFYFYCKILQLCDSQSFELIKRTMFETATKYILHNDSTWFRRFLVILLDREHIIISEHPSGKLPSGTLYVVKKFLLTLSRLSYNDLLLKFISDLQKYYQEGIIDRELIFQLVEYLNLNFVDYVKSSHDFFLLKRELGISDIETEKSTFRDVFKSHKQPIPQSCFIESGIIHACSTGTQEVSNYMQKLKSLFNSSTLPNFFRFFVNLIAAHLQSESKELLTFKLQIGGFILYLINDFLKSKYLQVKPDEFLELCKLHILLCSYDNATIEQERKQHFRKENIQFQINVLRIADGFSDYHLLAQRSKEFLIGNNRPNSPPDDELSAKVDEITRHFINVDLHVELLQPHEDLYTLMSQLHDMVKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WVC1</accession>
    <name>A0A8J2WVC1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">mRNA export factor GLE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Nuclear pore protein GLE1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Nucleoporin GLE1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">RNA export factor GLE1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN860_14840g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GLE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WVC1"/>
    <dbReference type="OrthoDB" id="420884at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.510:FF:000003">
        <property type="entry name" value="Nucleoporin GLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.510">
        <property type="entry name" value="GLE1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012476">
        <property type="entry name" value="GLE1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038506">
        <property type="entry name" value="GLE1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12960">
        <property type="entry name" value="GLE-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12960:SF0">
        <property type="entry name" value="MRNA EXPORT FACTOR GLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07817">
        <property type="entry name" value="GLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="10" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="147"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="201"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="150"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00075092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CDF87803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="537" mass="62172" checksum="826F825F9A8FF3D9" modified="2022-05-25" version="1">MRFVFDELYQFSDDEEKYENDESSPCSTPEPSPADFTFVNEVEEDIPRLKLPRQSSSVELVSEKLDPESEQLLSQLNLHSKLSFADELPIIIPNSRNNNNSMSRKPDISMAPQEAAYGTATLLSTLQESLASKMNKLEIDNRAQFQSVKRAKETAQEERRRKEEAELKRKKEMEIKEQEAREAQRRQLELERQQREEELRLKKERDEKERMAKKALEQEETRRRQAEEAKKLEEQAIRGKAVTDFKAIEKTFRHYKDKIASIKKDIVEPVKKMDVNMRNIISRHKRKINPKFGQLTNSNSQLQSIQGELFNLIDQTRENNLAYLWVLNFIAKALVHQSETEVRVKPQSALPLGRLALSLLIKYPELKELLMARWVKKCPFVIGYTCKIDTEQGRQNMGWKRSSDQKWEEDTSYDERMGGIVTLFAVMTRLPLPSEFINTTVHPLSISHSWHLLARISNTPTNLLTNTHFIVLGSWWDAAALQFYEAYGNQAAKLLQLVGDNLTSAVSEHKYAGAARLRILMEEWQLSGLQSFPEMAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WVD2</accession>
    <name>A0A8J2WVD2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_15170g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_15170g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WVD2"/>
    <dbReference type="OrthoDB" id="74314at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052816">
        <property type="entry name" value="Peroxisomal_Membrane_PEX28-32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010482">
        <property type="entry name" value="TECPR1-like_DysF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28304:SF1">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28304">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06398">
        <property type="entry name" value="Pex24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="TECPR1-like DysF" evidence="7">
        <location>
            <begin position="163"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="532" mass="60963" checksum="1CA4E45983C254C9" modified="2022-05-25" version="1">MSAPRKREVMKKYFSKRYEKVLDSLMVADTTAAVAALVNNDTQKATGLPNLEVVHGVASSLFNASLEKLKPLKEDMILEKDMESSEEFWKDENFKQEIYSEAPTICTETEEQVVTKLESKTRQHFIDIFVEKLIARMVPEKLPEREHFYESGTEEERLKDQTVSATKLTQNLKDLTAKMGGVFEVQDSIVRLLTWRNPSGTVTMLILLTMVFFNPMYLLVMPLLYVMYAMMIPGYMRRHPLRRTFYPIRKIPGKSLLKDLVTGGPRKKRISALAVQEAGYNAGGINPGEIRENANNGVEFVANLRDFQNATSSLLSFLASFEKFKYGTAGFKDEGYSTVVFLAYFVSFLTLVLISPFINWSLVVSLAMWSIMITTHPKISPKMKTFIGTGAMEDGKKSTGTSRKYDVILDESPEVRFVEIFELYEQGITPRHWTFFKLSNQVFDPLDTVRKLQKPPPGVAALKDVLPPKTWSFDDNADWEVDYCVEKWASERGLKLAVEDEFLVDDMFKRKRLTRKVLRYANPIKKPSYKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WWD8</accession>
    <name>A0A8J2WWD8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="13">BN860_04852g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_04852g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="504"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="540"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="589"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="613"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="644"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="755"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1271"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1301"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1329"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1453"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="145"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="848"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF88213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1494" mass="168591" checksum="667013807681E6BF" modified="2022-05-25" version="1">MNSLHNQPVEKNSLGEADSTPSFYDGGERHEFHNNEGLQEMPTQSESGSQSSPLSPEMAFKVRTLARTLSSKQISDDLRELESSDENGFNAKQIIHDFVRDAKQQGIEFRKTGVVMEDVTASGTDRGALEGTTFGDILCLPLTIYRAIRAARHRKLRKIIRGVNVLAKPGEMVLVLGRPGSGCSSFLKTAAGITDEFAGGVEGDISYDGVPQETMMKKYKSNVIYNGEMDVHFPYLTVKQTLDFAIACKTPAVRVNGVSRSEYINTTRDLYATIFGLTEVYDTPVGNEFVRGISGGQRKRVSIAEALAAKGTVYCWDNATRGLDASTALEYAHAIRILTDLLRSTVFVTIYQASENIYDTFDKVTVFHSGRQIYYGPAKEAKAYFERMGFECPARQATAEFLTAMTDPEGLHEVRSGFEGKVPQTADDLEKYWQNSPEFALLKRDISAYKEAVNGEATEKFFDETMHAEKSKYVRKRSYYTVSFFEQVRLCTIRGLQRIYGNKAYTIINIVAAVVQAFIAGSAYYNSPSSTNGAFSRGGVLYFSLLYYSLMGLANITFDHRPILQKHKTYSLYHPGAEALGSTFSGMPFRMIGLTLFLIILFFLSNLNRTAGTFFRVYFFLTLSSESIYALFESIAAICDNMSQATPIAGIVMFSISMYSTYVIQEPNMHPWFRWISYVLPVRYAFEGMLEAEFHGRHMDCGGSLVPSGPGYEDILSDNRVCAFVGSKKGQSWVLGDDYLKTQFGYKYWHMWRNWGIMFIFLVGYIVAKTLFTEIKRPTKGGGDALVFKKGTRKAVAREAGPGDIEALDDMNLHTDARENFSNDSDVTRKDDQLFDKEFQTFQSKGTFMWRDVCYTIPYTGSEKILLDHVSGFVAPGTITALMGESGAGKTTLLNTLARRNNFGVVTGDMLVNGCPIDMSFERRTGYVQQQDVHISEMTVRESLIFSARLRRPQSIPEEEKLRSVNTVMKLLDMEEYSEALVGDLGAGLNVEQRKKLSIGVELVAKPDLLLFLDEPTSGLDSQSSWAIVQLLRKLARAGQCILCTIHQPSATLFERFDRLLLLRKGGQTVYFGDIGENSSTVLSYFESNGFRPCSKSENPAEYILEAIGAGATASVEEDWHEIWRNSEESQEVDDKIKKLIEEPSSTAFNDTQLVDKPSKYATDYFFQFKHVWLRTNVMFWRDKQYLMSKMMLQVVSGLYIGWTFFNPGESYTGLQDTTFAAFMAVIVSAPSMKQIQARALASRELFEVRESKSNMFHWSLLLFTEYLAEIPYQFVFSAMFFCGMYFPLRVFFEASRSAVFFLNYSIMFQLYYISLGLMILYMSPNLPAASVIMGVIVTILIVFSGVVQPVSLMPGFWTFMWKVSPYTYFIQNMVAIVLHKKPVVCKTKELSLFDPPQGETCGQYMENFLQRAEGYISNPDATSNCAYCQLSVGDEYLAQRGVSYGNLWRNFGFYWAYIVFNLFAMVAIYYISSVRGVGQFHPIAIFRRFFKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WWS1</accession>
    <name>A0A8J2WWS1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="9">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_08130g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 9">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="9"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19670">
        <property type="entry name" value="UBR-box_UBR1_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044046">
        <property type="entry name" value="E3_ligase_UBR-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055194">
        <property type="entry name" value="UBR1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF24">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18995">
        <property type="entry name" value="PRT6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22960">
        <property type="entry name" value="WHD_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="10">
        <location>
            <begin position="90"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="8">
        <location>
            <begin position="90"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF88349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1836" mass="211600" checksum="62615886B3060F54" modified="2022-05-25" version="1">MNYGVTNIRDFLVHLPKLAKYNYNDAVSYLVFKVLDLLMRCPPGSVDWSSIVTILESERWHDGTYKRILQDIDWKLTITPASKNASHKDAMCSKQCYPTETVFYCFTCTTNPLYEICESCFDEEKHRGHLYTAKVVVRPEGRVCHCGDPFVFKEPRFAFLCKNEQNNGPRTGPQGYNESALSTIGDVLDYLIDVMIYFKEVKENAQPMEVARQENTNSPVQQDLTLLRDSYGQVDDLNEQDTVWALQIDEEDCQMHYMDLTAKIARILNKPTEYAITITNMLEDGASSVTILRSKDKSKLRKISLQFQNEGAKNHIRSIGDTFKRDLTEDLIEWLYNIFSDESVSLETRASLRLCMLDKWHSGLLSTKLTSNRLSCFTSKINLLGGFLVSYEQRDTFPWFEPWKFTFNAKDDRLQGIMADYNKRLRDTDIPNSVNRFHGLHGSRFQYLLTEFPNFLSRLSRHRLVRVFCMLFTITDEARQYLAAQYLDVYLALLYGTVASDSTGFKVSMMGILSQYTFQDPETTNLAIRSGFVQRALRFAFTLMAFNPEDLVAYLPIPLYYGCKLPNETIRNRRTIICFKDICVLMSTNTVAQELLEDEGILSAIIESFAEFNNILPLKRETSEHVEFENFDFSSYYFFFSSILIMTDGYVRGISLIMNPETRRKVVTRLINIAMEKEFDSLGRFRKELPSCTSPKGLLDPKISTSTLLKVQETICGHKTEVINFQVGVDTQNFFNPMSYLFKFILQWSQCGRYAPLPEGLKNYINFENLFCDKKKALYISEASLSTLVLLGQINVGFWVRNGTPITHQARMYTRYSMREFTYMSDIFNVQFSMVLADPSDFVVTFLSRWGLRNWANGFPMGDFPDAETTTAIVNQCFLLLIQLFTEIKSLSMVSSVDGFEKTLKAEIIHALCFKNCTYSQIMNFIPEHITKHAAFDLYLEKYANYTPPSGLMDCGIYTLKPEYREEVDPFYFGLSASKRYEAEKSIRSSMAIKNQINFDDTFIPAKKVINYLKNSPYSKLFAISSTETFGLFLKNSLDHINKFDFENLLPNVVYLIHLCVINNLNDFMKIFWHEYTSMDSAECFYHSIGSILYGFLLKDNFSNVHGTIREIFRYLSETAPHVNAGGYLQEQAHSFNPDILRSSNIVKSSKDEEFERKKKMARMKKEKVIRRLERQQMKFIENNSIPTTDEEEFKESSMDSVSGDIVGWEFPHDSCVFCKMPGEDDVFVYFSYQESNICDHNIDFTRIKETANTLLYFSTDSEDKKNKQKSFSDSENYGLSPQMRLTIQDAVMKTCGHGSHISCLGNHMKSIRAAHNQTTKNVPVAYGFGLLYCPLCNGLCNSFLPKLTNTSSRAASDFFENKFQAKAAEESSLLLSTSMKAARICEDLTGKANSQKIQPFEIINRIFVNTVLNTELSSRCCPASVAIHEMPNQRLLTLRLLSELKSFLYLNYTRNSDFIVNQEVGPQNLFMLQEKYPDLLLLASEAMEHPLYTTLNQLPTLSAPTFYLKELIKTKLFKDCITLSKELVRVQFYKEHPVRSPWSIAIDPNESSSHEAVLIAEIIKKHLLCLDPVIPDDILRKVECLKFHIHIFLAESLKIFLRRLCMIFHSIYAIELEYSKEETFLRETDFLLSYLRMPSFFNIIEEFFEIDLEKSLGSFREALNNEKTIEFIDKFTLTNARTAKLVKLPQSLSHFTFSEADYTLQKAMKDDLAICLFCGKTCHIQKSVALHGYVQGECTNHSRNECEVLSTYGVFLLMRSNAVYLSYGQRGCFYPAPYLHKHGEPDEDFKFNNPVYLHAKRYNHLINDVILGNMIPHIVFRLTDGNSDLGGWETM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WX62</accession>
    <name>A0A8J2WX62_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_11716g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_11716g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WX62"/>
    <dbReference type="OrthoDB" id="4069723at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="513"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="612"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="258"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="316"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="612"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF88501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="760" mass="82543" checksum="7B3AB24A0C3B7E59" modified="2022-05-25" version="1">MTKSNRGSRAGSGGQGSSRSPTAESVNKWKIPHYYRRSPSSASQHSNTGDSNGNSTPSTPNINVMNSPKKVLLEDRKKGKKKSGYRSKRSNKKNPGEMVFVNYTVQDGSSDNESVGSQGINSAEIPTPLPKKKSSRSRMLKIFGNSKNEPPQSPDAVTPAKRSYNPLYKYTSALPGRDGGINSDEEVVPTSQPLLSEKQHYDPLSAEVLDPFAANGQENSKFNNDFVVPDSHLMGSRTTDENDASIAFSKMFSRKRANTGGSMSSLNSLNTSLQQQPSQFALQQQHHHQPQQLRHSQSFSNLGNVVVPATQRNPSFSSVSSFNNKFSPIRTASPARPRSARASSGLRLSRDMSSFQDVPESSTPAIVSQEMFLDTQTSLKTKPTTGNKYKRKQDSMPEIHRIYTPSTATSNASGTPSSSLVTPPTFPAGYTAASSASSTPNALELSQINNSTSNSTVNNTNSATNLEPCTSHNMPSTIFYKDTSAELLLEDSDMPTPIENFPALKGIPRTTLEEKEEDEDGEPGISSRLVSTNDRTRPHVSYNEPIDYVQFAHGSSTSSSQVDSIMTNSLSATTTNSLMGGHYFQEHLASGQSCKELKTDLQNKLSARATSALNSFSQTKTEPQNLGSMGSSSANNEAHDPFYKMNLEFDFENPDSFFREPKSGTPTNDVKATVSGNADLNSNATFHERYGNAHKPNHHQTVSTSTRIASHGTSGFEDGGTSVPVTHDLFEHSDNNNVAAKSTSNFPFYNNGGVNDFSYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WXN8</accession>
    <name>A0A8J2WXN8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S03-00320g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_00320g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2015333at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04043">
        <property type="entry name" value="C2_Munc13_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052811">
        <property type="entry name" value="Glucose_resp_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010439">
        <property type="entry name" value="MUN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014770">
        <property type="entry name" value="Munc13_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014772">
        <property type="entry name" value="Munc13_dom-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47263">
        <property type="entry name" value="ADENYLATE CYCLASE ACTIVATION PROTEIN GIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47263:SF1">
        <property type="entry name" value="C2 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_7G02350)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06292">
        <property type="entry name" value="MUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51258">
        <property type="entry name" value="MHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51259">
        <property type="entry name" value="MHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MHD1" evidence="3">
        <location>
            <begin position="638"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="853"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="domain" description="MHD2" evidence="4">
        <location>
            <begin position="1060"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF88717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1315" mass="149926" checksum="1EEBB8C8ACB3B130" modified="2022-05-25" version="1">MSPSSKGKRSVSSDSAESDPSVSSKYKPGISQSTLYYLTLKLLLLEYINEPRFQKYQEVPKAAPPPIIRRTPRKNLSGAEEDSRIMQISNGFKSNSSKSPSEVDSLQDTLKQLENHLSGIAMKRIAVDDENLRRSLLKFYNDLFLNPCMTSTLESMERFEELIVYFTKAANGELNKLIIGNVRRELHAEVSYFIDLLIRLTNLPNELVQKLRQYKESDKPQKHRLKKHSAPYGEVHSHSSSSSSVPTSQSSYASNKEGTTPSKPTFKLQEITHVAFFQQLFEVDEAKLQKDIIRVIREPLGDIYCKELLEIGENIRKGVGALKIHNFPTEKDYNLWANIELSEIAYLTDKFTPEKRAAGFTSQPHSMIPENPREVFVALLCKIFQHERSTSMNNLNLSKSALFFLHRASKCWRVEFYSTLASLVYTAANLSILQDEELNEHLTENLLNFIHSKILRSEDQMDTSNWNDVDQTQWMINLVHTSNQCLMSIDNLISALYASTKPKFSPVLSFYYTYIDADPAMLIYKQRKTSTDARMAKKLRRAVFKTSEEFYISLLDKVPKDKSIEIQHIHNIGELIIEQIKSIQKRYNKPLLDVVNLPFECASVLIEAFASDAPTMIERVEKYTLAKSGEHVGPVDALEMYDMLKELRSIYKQVQPKRKFPFNLEKIFTKYLSRLCDGVSSKVLKVIKESVKNEKWEQANSEVAYSSSVLDIFKMINESIRLFKNLDWGSDYQIAKIITFLLKSFSDGLHFYANLVLKMIEDDLSDDSSEILLQEDDYKITNVASDKSKNSWVDGMRSALKNSQPVEIPKPYQFKPRTCVLLNDLDSVIEKLNDLDGQLNLEELSETIKKHERKHRKSKSISNNSSQQLHQLYTIRVIGAENIRGFSSIDGLSNPVLSLVDTSQQREIGKTKIIRKSVNPVWDEEFEVDIPFGNTRTIVLTVWHRPSAKLRKLNSYDVCGKCTFLLDAKRYTDDGFPNAVTLDLDTQGKVFLEVSLESEKLDAVFCMGRAYRTVSRALDRAIELIAHKFSTFVNFAFSRSTLRTVCGSSGTTVSPKALVYDAILPLFDYLNANLSILASELTQALLFRVMLRAWASILTAADSLLLPSLSMAKIKKMQSTKSIWGNAMSALGNTTVVGYGRPLTQREIEVIFVWLNALCVDFFHNEGEGPPLSDLKNRYYQSILLIPVYYDQSIPALKKEADRLFPEYTKYLHNKTLGVSSNRTTSTRSRTLARKQTVVANATKKNRKQAEMDVQRDESDPLERSAETIDIVLRTLMAKGELDYVQKQLSGRKKIRKNLATERKVQAAVRGQKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WZH8</accession>
    <name>A0A8J2WZH8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ZYBA0S04-03884g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_03884g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10265969at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000001">
        <property type="entry name" value="Paired amphipathic helix protein Sin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000002">
        <property type="entry name" value="Paired amphipathic helix protein SIN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000003">
        <property type="entry name" value="Paired amphipathic helix SIN3-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.11">
        <property type="entry name" value="Paired amphipathic helix"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013194">
        <property type="entry name" value="HDAC_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003822">
        <property type="entry name" value="PAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036600">
        <property type="entry name" value="PAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039774">
        <property type="entry name" value="Sin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031693">
        <property type="entry name" value="Sin3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346:SF0">
        <property type="entry name" value="SIN3A, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346">
        <property type="entry name" value="SIN3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02671">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08295">
        <property type="entry name" value="Sin3_corepress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16879">
        <property type="entry name" value="Sin3a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00761">
        <property type="entry name" value="HDAC_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47762">
        <property type="entry name" value="PAH2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51477">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase interacting" evidence="9">
        <location>
            <begin position="855"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="163"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="439"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="591"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1593"/>
            <end position="1645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="37"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="439"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="450"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="470"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1617"/>
            <end position="1629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF89420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1645" mass="186788" checksum="9F8D70604951503B" modified="2022-05-25" version="1">MSQIWNHSPPTGVASQVPTSDGSSEDNKRKNGENRQGKNIGNDGGSNSNEDNGHNNKTNNTDNPASRNSNDLPPQSANPSISNEHEKEGKAHFVLPSISDLSGEAASSPKSNATPITAAGTTSTGAPSNANPGGPPLLPHQSLRLPLMQGAYPTAPVLPGFSTAATAGDAQGPRAPNSLNSSLQSADDKNAVGVKPRSGSAGMSVASFDNPLPPSHQVQAPAQSQVPPLHYQMLSPPQKQQETHLQTQAQTQTQEQQNLQQQQHQIHEQYYRPLNVKDALSYLEQVKFQFIARPDVYNHFLDIMKDFKSQAIDTPGVIERVSTLFRGYPNLIQGFNTFLPQGYRIDCSKNPDEPIKVTTPMGPSTVAGVAEMNQVAMFQGQQSRIQQQHLQPLNLPSSQDYLSEQQQQIPFDATRNQQVLPSSSPSNVSSQPLVAQKVQANHVQPATVSQDQQQQQQQQQQQQASPTLSPPLQHASSQQSPQQSFADQTKKAVDVEFSQAISYVNKIKNRFSDQPDIYKHFLEILQTYQREQKPINEVYAQVTVLFQNAPDLLDDFKKFLPDSSASTSQQQRQQLQPVVQHQQTAAAYGYYSENPPASRQNLPPLGSFSPPPNGSAPPDYYREPPQAMSLPPMGRLENHPDQGAPPPYVMTQGMSNDALPMSSMRSPVGPAGVQSPVELSQQEQQLQQQQLQQEQLQLQIQQQHQQQQLQQLQQQEQQQQQQFQTQSSELAGQQDVEYVDIAVRPEIDLDPSIVPVVPEPTEPIEDSLSLVEETSFFDKAKKLIGNKQLYTEFLKILNLYSQDLLGVDGLVDKVEHYLGSNKELFTWFKNFVGYQDKPKHIENVVHEKHRLDLDMCEACGPSYKKLPKSDTFMPCSGRDEMCWEVLNDEWIGHPVWASEDSGFIAHRKNQYEETLFKVEEERHEYDFYIESNLRTIQTLETIASKIANMTEEEKANFKLPPGLGHTSLTIYKKVIRKVYDKERGFEIIDALHEHPAVAVPVVLKRLKQKDEEWRRAQREWNKVWRELEQKVFFKSLDHLGLTFKQADKKLLTTKQLISEISSIKIDQTNKRIHWLTPKPKSQIDYDFPDKQIFYDILSLLEVFLNHTSNYSNPDKERLKDFFKSFLSLFFFIPSGEIREGMQKRAHSSPKDTLEETKECDASTGKRSREIDFSLRDILHRNKYQKLKRRLDGDNFSGIHSEEGLVDEQEYEDEEEEVIRQEAKKPWLLGNIVEEANAEGVINNRRSFNLFANTTIYVFFRHLTTLYERLLEVKNLDEEVTKEINSRKVTQFAKDLNLISEQLKNMGLDFAGLDAYQQLLHLSKRLIEGDIEHQWFEESLRQAYNNKAFKFYTVDKVIQSLVKHAHSIMTDVKCSEIMILFEKDRALSTTSAKDQILYRMQARSHMSNTENMFRIEYNKHTHHVCIQYIAIDDLTLAEAKSLEDKWKYYVTSYSLSHPTEGVLHEDLRVPFLEKIIEDERDYDDENSENPKFSPEGVCKSTLRIKIDPETYFLEIEPGTYDVFSRKSVNRFPQIKNGQAFLAKINRKKGLITNYMDGAKGWRKHLDDNVTQSTKGKLAYAEKYGTLRGFGDLKNDAGDAEQDSTQTEGLKQENDFNSRDSVTNGVTSQAGSEDRGGNDTTAEEVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X1Y1</accession>
    <name>A0A8J2X1Y1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S06-06656g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_06656g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X1Y1"/>
    <dbReference type="OrthoDB" id="4069534at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="546"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="572"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="632"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="656"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF90356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="674" mass="74662" checksum="6D7BE994C42770C5" modified="2022-05-25" version="1">MVRNEELEDFLKRVENYDSQRRETGGMNATNHGAYSQESIERARQLLQNGIAIVDDTPIGSTLATSDLAYKSAYNYERTFSPRRVMSQEKTYVVSEQDYLLLQSLKAKEKSPPPLPSRGRARELCLERILAEEKPPALPTRRYYSLSVSTDDSYNASILADGSNRLKGSKPPVPPKSRSTLRTLEIRTESEAKDVSVTPDSSKEEKRPSLGEEHNLEKKTRNSERSELKNSPRRYNLNAELEDSVKIAAVNSSIQQVPQQSPIDFLDSVQRKQVEYHTNTPSFGVTRTSEYDIDGFTHSAAKSTSQFNPARTAKPIQFIKNGTSDSESFISSAIKVSPNSHNVSENHINFTVPSNPSSESFLSSALKVLPPSKPIAPPKPIGLTKQTEPRTIDLNRPQPEKPLKPAKLAIKLKPKVPPKKSEIIIPKLRHVDSPPPPPFNKGSPVHETNFTNKLKKVEPPTVSEKSPNLPEALAKLGHLSRPPASPKRQDEVPEALTKLGHLSKPIVLPKPEGGLPEALTKLGTLSRPTISPKRENDIPEALSHLERLNKVNKQPPVPARKISMAEALQRAQELKAQKRSEQATKPDPEQPKDMKTELGAVLMAQKLRSTKPFLSGRSHTEPSSNNSSTTSLGRTQSTPITTFADQNATSNTLMHATKKRAKGPKRKLPSNVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X2E1</accession>
    <name>A0A8J2X2E1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Pentafunctional AROM polypeptide</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="23">3-dehydroquinate synthase</fullName>
                <shortName evidence="23">DHQS</shortName>
                <ecNumber evidence="23">4.2.3.4</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="23">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
                <ecNumber evidence="23">2.5.1.19</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="23">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
                <shortName evidence="23">EPSP synthase</shortName>
                <shortName evidence="23">EPSPS</shortName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="23">Shikimate kinase</fullName>
                <shortName evidence="23">SK</shortName>
                <ecNumber evidence="23">2.7.1.71</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="23">3-dehydroquinate dehydratase</fullName>
                <shortName evidence="23">3-dehydroquinase</shortName>
                <ecNumber evidence="23">4.2.1.10</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="23">Shikimate dehydrogenase</fullName>
                <ecNumber evidence="23">1.1.1.25</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="23" type="primary">ARO1</name>
        <name evidence="31" type="ORF">BN860_03114g</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="32">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="22 23 24">The AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23 24">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:21257"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23 24">
            <text>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate = 3-dehydroquinate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21968"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="EC" id="4.2.3.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21 23 24">
            <text>shikimate + ATP = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23 24">
            <text>shikimate + NADP(+) = 3-dehydroshikimate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="23 24">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="23 24">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="23 24">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="23 24">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="23 24">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 23 24">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 23 24">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="23 24">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 23 24">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="23 24">In the 2nd section; belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the 2nd section; belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="23 24">In the 3rd section; belongs to the shikimate kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="23 24">In the 4th section; belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="23 24">In the C-terminal section; belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="24">In the N-terminal section; belongs to the dehydroquinate synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the shikimate kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="23">In the N-terminal section; belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.4" evidence="23"/>
    <dbReference type="EC" id="2.5.1.19" evidence="23"/>
    <dbReference type="EC" id="2.7.1.71" evidence="23"/>
    <dbReference type="EC" id="4.2.1.10" evidence="23"/>
    <dbReference type="EC" id="1.1.1.25" evidence="23"/>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="197068at2759"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00085"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00502">
        <property type="entry name" value="DHQase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08195">
        <property type="entry name" value="DHQS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01065">
        <property type="entry name" value="NAD_bind_Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00464">
        <property type="entry name" value="SK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1090.10:FF:000007">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000135">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1970:FF:000007">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001256">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.65.10.10:FF:000007">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="entry name" value="Enolpyruvate transferase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03143">
        <property type="entry name" value="Pentafunct_AroM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00109">
        <property type="entry name" value="Shikimate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018508">
        <property type="entry name" value="3-dehydroquinate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016037">
        <property type="entry name" value="DHQ_synth_AroB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030960">
        <property type="entry name" value="DHQS/DOIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056179">
        <property type="entry name" value="DHQS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001381">
        <property type="entry name" value="DHquinase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008289">
        <property type="entry name" value="Pentafunct_AroM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041121">
        <property type="entry name" value="SDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010110">
        <property type="entry name" value="Shikimate_DH_AroM-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000623">
        <property type="entry name" value="Shikimate_kinase/TSH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023000">
        <property type="entry name" value="Shikimate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01356">
        <property type="entry name" value="aroA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01357">
        <property type="entry name" value="aroB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01093">
        <property type="entry name" value="aroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01809">
        <property type="entry name" value="Shik-DH-AROM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090">
        <property type="entry name" value="AROM/DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090:SF5">
        <property type="entry name" value="PENTAFUNCTIONAL AROM POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01761">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24621">
        <property type="entry name" value="DHQS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01487">
        <property type="entry name" value="DHquinase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18317">
        <property type="entry name" value="SDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000514">
        <property type="entry name" value="Pentafunct_AroM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01100">
        <property type="entry name" value="SHIKIMTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01028">
        <property type="entry name" value="DEHYDROQUINASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00104">
        <property type="entry name" value="EPSP_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00885">
        <property type="entry name" value="EPSP_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01128">
        <property type="entry name" value="SHIKIMATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 23 24" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="17 23 24" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="14 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 23 24" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 23 24" id="KW-0418">Kinase</keyword>
    <keyword evidence="18 23 24" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 23 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19 23" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="15 23" id="KW-0521">NADP</keyword>
    <keyword evidence="11 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16 23 24" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 23 24" id="KW-0808">Transferase</keyword>
    <keyword evidence="13 23 24" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="3-dehydroquinate synthase N-terminal" evidence="27">
        <location>
            <begin position="73"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="3-dehydroquinate synthase C-terminal" evidence="30">
        <location>
            <begin position="186"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Enolpyruvate transferase" evidence="25">
        <location>
            <begin position="416"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="domain" description="Shikimate dehydrogenase substrate binding N-terminal" evidence="28">
        <location>
            <begin position="1311"/>
            <end position="1393"/>
        </location>
    </feature>
    <feature type="domain" description="Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase" evidence="26">
        <location>
            <begin position="1430"/>
            <end position="1504"/>
        </location>
    </feature>
    <feature type="domain" description="SDH C-terminal" evidence="29">
        <location>
            <begin position="1556"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="region of interest" description="3-dehydroquinate synthase" evidence="23">
        <location>
            <begin position="1"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate dehydrogenase" evidence="23">
        <location>
            <begin position="1306"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for 3-dehydroquinate synthase activity" evidence="23">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for 3-dehydroquinate synthase activity" evidence="23">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <feature type="active site" description="For EPSP synthase activity" evidence="23">
        <location>
            <position position="853"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for 3-dehydroquinate dehydratase activity" evidence="23">
        <location>
            <position position="1198"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; for 3-dehydroquinate dehydratase activity" evidence="23">
        <location>
            <position position="1227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="43"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="78"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="109"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="134"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="174"/>
            <end position="177"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="189"/>
            <end position="192"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
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        <location>
            <begin position="272"/>
            <end position="276"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
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        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
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        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="895"/>
            <end position="902"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00044633"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00054455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRNR" id="PIRNR000514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF01761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF08501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF18317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF24621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="EMBL" id="CDF90529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1588" mass="174843" checksum="7F5FED7F6CF74388" modified="2022-05-25" version="1">MEFLQKVRILGRESIHVGYNAHAHMVQTIINECASSTYVAITDTNLLKVPYYHDFVQELRSNLPAESRLLEYSVKPGEANKTRATKAEIEDYLLSQGCTRDTVIIAVGGGIIGDMIGFVAATFMRGVRVVQVPTSLLSMVDSSIGGKTAVDTPLGKNFIGAFWQPQFVLVDVKWLETLPRREFINGMAEVIKTACIWNSEEFERLEANANLFLEVVNNSKLVKVGNPQTSEVHEISYTNVQQMLEHTFKLVLESIKVKAEVVSSDERESNLRNLLNFGHSIGHAYEAILTPQALHGECVSIGMVKEAELSRYLGILSPTQVSRMSKILVAYGLPVSKDEKWFQDLTLHKKTPLEELLKKMSIDKKNDGSKKKVVLLESIGKCYGKSAHFVADEDLRFILTDETLVYPFSNIPIDQVKTIVPPGSKSISNRALILAALGEGTCKIKNLLHSDDTKHMLAAVQALKGASITWENNGETVVLTGHGGKTLTACPDPLYLGNAGTASRFLTTVAALVNSSSTQDYVVLTGNSRMQQRPIGPLVDSLRINGTKIEYLKNEGSLPLKIYTNTPFKGGRIELAATVSSQYVSSVLMAAPYAETPVTLALVGGKPISQLYIDMTIKMMEKFGIKVQVSTSEQYTYHIPKGHYINPGEYVIESDASSATYPLAFAAMTGTTVTVPNIGSESLQGDARFAVDVLRPMGCTVHQTAVSTTVTGPPRGSLKPLCHVDMEPMTDAFLTACVVAAVAHDNNKESSNVTTIEGIANQRVKECNRIEAMGTELAKFGVVTKELLDGIQVHGVKSLNLLKIPADSSGPIGVKTYDDHRVAMSLSLLAGMVSIGDREDENTTPVRILERSCTGKTWPGWWDVLHTELGAKLDGAEPVDYLSQKNIKKSIVLIGMRAAGKSTVGRWCAQTLGYQLVDLDVLFEKQYGKGTIKDYVAKHSWEKFREEESKLFKETIKMYGDGGYVFSTGGGIVESKIARDHLKKYVAEGGVVLHLHRDIEETIVFLQSDPTRPAYVEEIRDVWQKREKWYHECSNFNFFAPHCSNEAEFQNLRKSFGNFITTITGNNVIEIPTKRSAFVCLTFDDLTERLDSLPSIVYGCDAVEVRVDYLARFDSEFIFKQLSVLRNATSNLPIIFTVRTKKQGGKFPDDDHTGLAKLFEMALKAGVEYIDLELTLPTDLQYKILNSKGYTKIIGSHHDFAAQYSWADPEWENRYNQALSLNVDVIKFVGTATDFEDNLALEKFREEHKVKPLIAINMGEKGKVSRVLNTVLTPITSEHLPNAAAPGQLTLAQINQIFASMGGIIAKKFFVVGKPVAHSRSPLLHQTGYELTGLPHHFSRFETDSANVVKEKLLDGEPKLGGLAVTIPLKLDIMQYMNELTDAVKVIGAMNTVIPMGNGKFKGDNTDWLGIKNSLTSNGVPDSTPHVAGLVIGAGGTSRAAIYALHDLGCSKIFIINRTVSKSIALKDSFPKEYNIVAVETSEEVDTIEERIGVAVSCVPADKPLDSGLLSKLERFLHKGARSSFVPTLLEAAYKPSITPIMTLARDKYQWQVIPGTQLLVHQGVAQFKIWTGVSAPFQAIFEAVTRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X4K8</accession>
    <name>A0A8J2X4K8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="22">ZYBA0S11-00210g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">BN860_00210g</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 24">
            <text>N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP = N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13501"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hydrogencarbonate + acetyl-CoA + ATP = malonyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11308"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <dbReference type="EMBL" id="HG316464">
        <property type="protein sequence ID" value="CDF91322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="14612at2759"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004075">
        <property type="term" value="F:biotin carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.460.10:FF:000001">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1770.10:FF:000001">
        <property type="entry name" value="acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000005">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000010">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.460.10">
        <property type="entry name" value="Biotin dependent carboxylase carboxyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1770.10">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049076">
        <property type="entry name" value="ACCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049074">
        <property type="entry name" value="ACCA_BT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013537">
        <property type="entry name" value="AcCoA_COase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728:SF3">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08326">
        <property type="entry name" value="ACC_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21385">
        <property type="entry name" value="ACCA_BT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0092">Biotin</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="19">
        <location>
            <begin position="55"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="18">
        <location>
            <begin position="202"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="17">
        <location>
            <begin position="690"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="20">
        <location>
            <begin position="1482"/>
            <end position="1818"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="21">
        <location>
            <begin position="1822"/>
            <end position="2137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1190"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="2033"/>
            <end position="2060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1190"/>
            <end position="1199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CDF91322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2229" mass="248580" checksum="7C8BC65A8AC65E29" modified="2022-05-25" version="1">MSEESLFEVTQRTEVPDYSKEHAKLPPNFIGLRTVDRAEESPLKDFVKTHGGHTVISKILIANNGIAAVKEIRSVRKWAYETFGDDRAVQFVAMATPEDLEANAEYIRMAEQYVEVPGGTNNNNYANVDLIVEIAERADVDAVWAGWGHASENPDLPESLAKSKRKIIFIGPPGNAMRSLGDKISSTIVAQHAKVPCIPWSGTGVDQVHIDEDTQLVCVDDEVYQRGCCSSPEGLKCAQEIGFPVMIKASEGGGGKGIRQVHRAEDFIALYKQAANEIPGSPIFIMKLAGKARHLEVQLLADQYGANISLFGRDCSVQRRHQKIIEEAPVTIAKPETFREMEKAAVRLGKLVGYVSAGTVEYLYSHEDDKFYFLELNPRLQVEHPTTEMVSGVNLPAAQLQIAMGIPMHRIRDIRILYGMNPQQSTEIDFGFTNESSSTTQRKPMPKGHCTACRITSEDPNEGFKPSGGSLHELNFRSSSNVWGYFSVGNNGGIHSFSDSQFGHIFAFGENRQASRKHMVVALKELSIRGDFRTTVEYLIKLLETEDFEKNTITTGWLDDLISQKMTAEKPDPTLAVVCGAATKAFIASEASCQSYINSLKRGQVPSKALLQTMYPVEFIHESKRYKFTVAKSADDRYTLFINGSKCEVRVRKLSDGGLLIAIGGKSHTIYWKDEVSATRLSVDSMTTLLEVEDDPTQLRTPSPGKLVKFLVENGDHVEGGQTYAEVEVMKMQMPLIASESGIVQLLKQPGSTIAAGDIIAILSLDDPSKVKHAMPFEGMLPDYGPPVIEGTKPVYKFRALENTLQNILNGYDNQVIMNASLQQLIQVLRDPELPYSEWGQQISALHSRLPSKLNDHLNQLVERSARRKAVFPARQLNKLLESTVKQSEVESSMLGTVVAPLVEIANRYANGLAAHEHTVFVKFLEEYYNVEKLFNSTTEENVILKLRDENVDNLEKVVVIVLSHAKVAAKNNLILAILKHYQPLCKMSSEVSSIISGPLQHIVGLEGKATAKVALQAREILIQGALPSVKERSEQVEHILKSSVVRPTYGATNPKRSEPDMEVLKDLIDSNYVVFDVLTPFLSYPDPSVAAAAAQVYVRHAYRAYTIGEVTVCEGTTSPICKWKFQLPSASFTSIPQIKTKFGMNRAISVSDLTYVADPENPPLRTGILVSSEHLDDVDDALAQSLETIPTTNSSTAASAGPSPDRSGSPTSLSNVVNVYVQSTDGFESEADVLTRLREILDTNKQELIASGIRRITFIFGYSDGSYPKYYTFKGPAYDEDKTIRHIEPALAYQLELGRMSNFNIKPIFTENRNIHVYEAVGKSTPLDKRFFTRGIIRTGRIRDDISIQEYLTSEANRLMSDILDNLEITDTSNSDLNHIFINFSSVFDVSPEDVEAAFGGFLERFGKRLLRLRVASAEIRIIIKDPKTGAPVPLRALINNVSGYVVKTELYTEVKNANGDWVFKSLGKPGSMHLRPIATPYPVKEWLQPKRYKAHLMGTTYVYDFPELFHQAVVSQWHKLSPKTKLNDNFFVANELIEDENGELTEVDREAGANTIGMVAFKITAKTPEYPRGRQFVVVANDITFKVGSFGPQEDEFFNKVTEYARKRGIPRIYLSANSGARIGIAEELVPLYQVSWKDPNDATKGYDYLYLTTEGLEELKKYGKDHSVITERIVENGEERHLIKAIIGVDDGLGVECLRGSGLIAGATSKAYQDIFTVTLVTCRSVGIGAYLVRLGQRAIQVEGQPIILTGAPAINKIMGREVYTSNLQLGGTQIMYSNGVSHLTAPDDLAAIEKILAWLSYVPAKRNMPVPILENEDKWDRDIEYYPPSDEPYDVRWMIAGRETPEGFERGLFDRDSFMETLSGWAKGVVVGRARLGGIPMGVIAVETRTVENLTPADPANPASTESTTQEAGQVWYPNSAFKTAQAIRDFNNGEQLPLMILANWRGFSGGQRDMYNEVLKYGAYIVDALVDYKQPIMIYIPPTGELRGGSWVVVDPSINSEHMEMYADVDSRAGVLEPAGMVGVKYRREKLLGTMARLDDTYRSLKEQLADASSSSEEHKAISKKLAIREQQLLPIYNAISVQFADLHDRSARMQAKGVIRKELHWVNSRRFFFWRLRRRLNEEYLMRRLDAELPLASRLEKIARLRSWYPASVDHENDRQVATWIEENYQILDDHLKALKLESFAQRLAASLRGDHDNVMTGLAEVLKMLSAEDKEKISKSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X4P7</accession>
    <name>A0A8J2X4P7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase</fullName>
            <ecNumber evidence="6">2.1.3.2</ecNumber>
            <ecNumber evidence="5">3.5.1.2</ecNumber>
            <ecNumber evidence="20">6.3.4.16</ecNumber>
            <ecNumber evidence="4">6.3.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">BN860_05600g</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="31">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="25">Multifunctional protein that encodes the first 2 enzymatic activities of the de novo pyrimidine pathway: carbamoylphosphate synthetase (CPSase; EC 6.3.5.5) and aspartate transcarbamylase (ATCase; EC 2.1.3.2). The CPSase-function is accomplished in 2 steps, by a glutamine-dependent amidotransferase activity (GATase) that binds and cleaves glutamine to produce ammonia, followed by an ammonium-dependent carbamoyl phosphate synthetase, which reacts with the ammonia, hydrogencarbonate and ATP to form carbamoyl phosphate. The endogenously produced carbamoyl phosphate is sequestered and channeled to the ATCase active site. ATCase then catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">In the 2nd section; belongs to the CarB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the 3rd section; belongs to the metallo-dependent hydrolases superfamily. DHOase family. CAD subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">In the C-terminal section; belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">In the N-terminal section; belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="6"/>
    <dbReference type="EC" id="3.5.1.2" evidence="5"/>
    <dbReference type="EC" id="6.3.4.16" evidence="20"/>
    <dbReference type="EC" id="6.3.5.5" evidence="4"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1924069at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005951">
        <property type="term" value="C:carbamoyl-phosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01423">
        <property type="entry name" value="MGS_CPS_I_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000002">
        <property type="entry name" value="Aspartate carbamoyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000025">
        <property type="entry name" value="Bifunctional pyrimidine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000005">
        <property type="entry name" value="CAD protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000011">
        <property type="entry name" value="CAD protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000004">
        <property type="entry name" value="Carbamoyl-phosphate synthase (glutamine-hydrolyzing)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1030.10:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000036">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000009">
        <property type="entry name" value="Carbamoyl-phosphate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="entry name" value="Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CPSase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CPSase_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058047">
        <property type="entry name" value="CPSase_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002032">
        <property type="entry name" value="PRK00856.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003671">
        <property type="entry name" value="PRK05294.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009455">
        <property type="entry name" value="PRK12815.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF5">
        <property type="entry name" value="CAD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25596">
        <property type="entry name" value="CPSase_L_D1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00101">
        <property type="entry name" value="ATCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="Carbamoyl phosphate synthetase, large subunit connection domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-grasp" evidence="28">
        <location>
            <begin position="559"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="28">
        <location>
            <begin position="1096"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="29">
        <location>
            <begin position="1353"/>
            <end position="1504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00043968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00044063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00058513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00081752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="CDF87404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2216" mass="244896" checksum="E4116DC0B8AAC86C" modified="2022-05-25" version="1">MSAIVPTSPVTPPMSSTGDRLAALELKDGTTLVGYSFGAAKPSAGELVFQTGMVGYPESITDPSYEGQILVITYPLVGNYGVADTKVKDELLEQLPRYFESNRIHVAGLVIAHYTEKFSHWLAHSSLSDWLKKEGVPAVYGVDTRALTKHLRDSGSMLGRLCLQSSEGQVDLAQQNWQSFFDVPSWVDPNVKNLVAEVSVKEPQLYLPPPDKVNLQKGPDGKVIRILAIDVGMKYNQIRCFVKRGVELKVVPWNYDFTKEQYDGLFISNGPGDPSVLHDVSSRLASVLQSKRTPVFGICLGHQLLARANGFSTVKMKFGNRGQNIPCTSTISGRCYITSQNHGYAVDEKSLTGGWKPLFTNANDGSNEGIYHTELPYFSVQFHPESTPGPRDTEFLFDVFIQAVMSHKSTGVLKSIQFPGGNVEENRKAHPRIEAKKVLVLGSGGLSIGQAGEFDYSGSQAIKALREEGIYTILINPNIATIQTSKGLADKVYFLPVTPEFVRKVILHERPDAIYVTFGGQTALSVGIAMKDEFEALGVKVLGTPIETVITTEDRELFAAAIAEINEKCAMSRAASSLDEAVAASKEIGFPVIVRAAYALGGLGSGFADNEKELIDLCNIAFASSPQVLVERSMKGWKEIEYEVVRDAFDNCITVCNMENFDPLGIHTGDSIVVAPSQTLTDEDYNMLRTTAVNVIRHLGVVGECNIQYALNPFSKEYCIIEVNARLSRSSALASKATGYPLAYTAAKLGLNIPLNEVTNSVTKSTCACFEPSLDYCVVKMPRWDLKKFTRVSTKLSSSMKSVGEVMSIGRTFEEAIQKAIRSTEYKNLGFNETDLDVDVDYELNNPSDLRIFAIANAFANLGYSVDKVWEMTNIDKWFLNKLYELFNFSKKIASYGTKEQLSPVVLKQAKQLGFDDRQIAKFLNSNEVAIRRLRKELGITPFVKQIDTVAAEFPAFTNYLYMTYNAATHDVQFNDHGVMVLGSGVYRIGSSVEFDWCAVTAVRTLRANNIKSIMVNYNPETVSTDYDEADRLYFETINLERVLDIYEAETSGGVIVSMGGQTSNNIAMSLHRENVKILGTSPEMIDGAENRYKFSRMLDQIGVDQPAWKELTSIEEAEDFADNAGYPVLVRPSYVLSGAAMNTVYSKADLESYLNQAVEVSRDYPVVITKYIENAKEIEMDAVARDGELIMHVVSEHVENAGVHSGDATLIVPPQDLDPETVDRIVVATAKIGKALKITGPYNIQFIAKNNEIKVIECNVRASRSFPFISKVVGVNLIEMATKALMGLPVTPYPVEKLPDDYVAIKVPQFSFPRLAGADPVLGVEMASTGEVACFGHSKYEAYLKSLLSTGFRLPKKNILLSIGSYKEKQELLPSVVKLYNMGFKLFATAGTADFISEHGIPVQYVEVLNQEDEEKAEYSLTQHLANNKIDLYINLPSANRYRRPASYVSKGYRTRRMAVDYSVPLVTNVKCAKLLIEAISRHLPLDVSERDAQTSHRTITLPGLINVATFVPNASNVVDGPAEMRETTRLSLEAGFTYCQIMARAISGPIISDVHSLKAAISAAQNSTYTDVSFTVAGTVNNAGYVAQTAGDVSALFLPFRELTNKVSTVAELLKNWPAGNQIIAEAKTSDLASVMLLTALQSRPIHITGVSNKEDLDLIITVKEKDSRLTCDVNIHALFVSRDDYPEAVFLPTKEDQEFFWRNLDSIDVFSIGALPTALAHVTGNKVDVGLGIKESLPLLLSAVDEGKLTIDDIVQRLHDNPANIFNIPEQRDAVVEVDLDYSFNHTKRWSPYTKGGLNGGIDRVVINNETVVLSGDLVVPEPKGKVVITSKTIPSTPSAQNDTPLNVQATKRRFSFSNERRGSTTSIEGYEELSQPLEQRLMSSRPPKELTAPGAIQSLIRSNNPFKGRHILSVRQFKRSDFHALFAVAQELRAAVEREGVLDLMKARVLTTIFYEPSTRTCSSFIAAMERLGGRSVNINPSASSVKKGETLQDTIRTLACYSDAIVIRHPDEMSAHTAAKYSPVPIVNGGNGSREHPTQAFLDLFTIREELGTVNGITVTFMGDLLYGRPVHSLCRLLQHYQVRINLISPEQLRLPAALRKELSDAGLLGVESEILTPEIISKSDALYCTRIQKERFENKDQYERLKDVYIVDNKMLAHAKEHMAVMHPLPRVNEIKEEVDYDHRAAYFRQMRYGLFVRMALLAMVMGVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X4Y2</accession>
    <name>A0A8J2X4Y2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BN860_08042g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_08042g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X4Y2"/>
    <dbReference type="OrthoDB" id="289913at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098588">
        <property type="term" value="C:bounding membrane of organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025941">
        <property type="entry name" value="Vps8_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 8 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23410">
        <property type="entry name" value="Beta-prop_VPS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12816">
        <property type="entry name" value="TPR_Vps8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23413">
        <property type="entry name" value="zf_RING_Vps8_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="CHCR" evidence="3">
        <location>
            <begin position="491"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 8 central" evidence="5">
        <location>
            <begin position="535"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF87511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1268" mass="144025" checksum="DE8FCC29FDAE151A" modified="2022-05-25" version="1">MKNEIAPDNSNMNSASSKVSRSDLSFLDYRAYTRLSEKSTESAEYSLRAKPNGNFQNCDSFFDDQANSSSILKWTILANIYPISGAYGGLTCIYSTKSCYVLGTIKGAILIFSTKQVLLHTLVPQIMNESTANYLRSAVQKIVVSADGTHLAASYESGECFIWNLNADTEVKNELDESREGTSSIRAILHITDHKGRQINGIGFMGLRHTALIVSDNSGCIFYHSGFRTRFWSLTYSSKQVLNISSGEKLIHSQSVPNVEGQPLHMIAVLTTSRFAIISTAPRLTTLFLQNVGDTTPTGVSSNGCLSWHESGSKVALSLDTEVTVVTIQESSPFSVRKTVTKLEEVAVLLHWLTQDLLGVLTESCQFFILDVSGNSKIVGTFDFLIHDLLMPPNKHFTICDRKLQLLTNSALKIGKFASWSDIVLNQVQAGNYIGALKFIKSWLLEHVPLWEIIRLKKIHSEREQELVKPLYNLALAALKYVINGPDTKYDDVYEVLSLVLSTLNSFEDESLKKETTSSFLEKSMNFFHETSISVFYEVLMNLIMEESLSALPAILFKSMLTFYANEEKLAIVEDMVVMLDPRTLDIDLAVKLCQKYGLFSLLIYIWNNIFDDYSLPLVDAVLRINDRHEEVSIFKTENVNSVNCIFDYLSFILTGRQYPRNQPIAPIEKQIEGKSGLYYMLFSGTCIQWPPKSGKKVHTKLDSNEEPAFPYFKLLLDFNTRRMLSTLNEIFEDSLLNEGYTDTGREDDHQINITRQYMIDVIIDIMREKYACADKALMAIFIACNIAKYPQFIELSSRALDGVATVICELDEPDLAADSQRALECLLSVYIPSNPDKFITTLKEKCFKRVLFILYIKTKKYSEILPLALSSPSTNIDYGKDFSTIIHFVLFETKSDALEHIRIAGLIRENFDTLVKKLGSQNAADVFENFDSDLHLCISQVHEGIIQQEYLEELYLHGILERPQLQHLKNLYFEVACQYKEKNCLLSWLDGINFKNLDVEHAINLLLSKNNYEAAAVIHQRLQAYTMVVDDMIFCIVGWFKQGGNGYETLGNYLDMAIEAIGRSKEEKQSNWTKLIACFMKLYGKYKGDTNSKQSCNRAFQQLFVRLAMAEAPEKDRKKSQLWNILTSALEQQEVIMMRAQDLRTLLSDVLTAYEIEWHIYNIILTIIQKSSVGLGNLYESTLMEGWSIINDECVICGKKLWGLGLSTLNFLVWERKRFHEGNKPFECTDADLTTVSFGCHHLFHKKCLQNLGQRDDKYFCLTCKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5I7</accession>
    <name>A0A8J2X5I7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BN860_02938g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_02938g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. TRA1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5570127at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05163">
        <property type="entry name" value="PIKK_TRRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000044">
        <property type="entry name" value="NuA4 histone acetyltransferase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046807">
        <property type="entry name" value="Tra1_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046805">
        <property type="entry name" value="Tra1_ring"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF1">
        <property type="entry name" value="TRANSFORMATION_TRANSCRIPTION DOMAIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20175">
        <property type="entry name" value="Tra1_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20206">
        <property type="entry name" value="Tra1_ring"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAT" evidence="4">
        <location>
            <begin position="2604"/>
            <end position="3159"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="3">
        <location>
            <begin position="3358"/>
            <end position="3716"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="5">
        <location>
            <begin position="3696"/>
            <end position="3728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="516"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2032"/>
            <end position="2068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2032"/>
            <end position="2054"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF87285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="3728" mass="429612" checksum="C4F336D9B63DF046" modified="2022-05-25" version="1">MSFGVQIDHFVAKFSEEDGTLQTKHSSLSELCDIIEYLSNPEDYQGLLHAVIPLLLQQLEKISISFDVSSPENKLRNSILKVFNRLPINQSFEPYVGQVLQVVMGALPRENEENGVLCMKILTTLFKSFKSTLQDKVEQFIQIIVHIYKNTPEAVSKTFQSSDNGASSDDELRDEQMLNEEVDTGESTGNQTSLTSVGPKDVKAAMYSFKVLSECPITMVTLYSSYKQLTTTSLPQFMPLVMDLLNMQVEQQRVAREEAQARGERYTAVSPDIKNREAYCEFILAQIKATSFLAYVFIRGYAPEFLQNYVGFIPDLIMRLLQDCPPELSSARKELLHATRHILSTNYKRLFLPKLDYLFDERVLIGTGFTTHETLRPLAYSTVADFIHNVRAELRLDDIEKTIQMYTRFLLDQSLALTVQIMSAKLLLNLVERILKLGKENPQEAPRAKKLLMIIIDAYVNRFKVLNRQYDTIMKYHKKHERVKNAKTKQLQEAVQIDNKDSDELMDNILHPAGASCSRPEAKEKNETTENTEPGNEVLVDDDVENQKCGMYDIKNHAPILLTPVSTNDPIKDAFYLYRTLMSFLKTIIHDLKVFNPPPAEYTIANPKLWVSVSRVFSYEEVIVFKDLFHEYIVGLRFFSSNAGKPNPPSKKHFDITMPSLPVSATKDGRELMDYLAFMFMQMDSSTFNEIIEGEMEFIYESMLEDSALLHVAQSFLTSEITSPNFAGILLRFLKSKLKDLGNVDFNKSNILIRLFKLSFMSVNLFPTINEIVLLPHLNDLILDSLKYSTTAEEPLVYFYLIRTLFRSIGGGRFENLYRSIKPILQVLLQSLNGMILTACLPHERELYVELCITVPVRLSVLAPFLPYLMKPMVYALEGYPDLVSQGLRTLELCIDNLTAEYFDPIVEPVVEDVTKALFKLLRPQPFNHTIXTALRILGKLGGRNRRFLNQASDLKAQSELDIEINAFFKMNGLVEEVPLSITPGIESALIILEDYRVDSGYKQSAFKYLSSVLFLFVKSSTHFGDNYDKTLEAAIHAITMEKIEIRPDFKAEPVKNNRTFHNQEKLFLRLLQAMFFSYSIAELKEEASKILDRLIDHFSLLQVNNTLLDKRNYSSAFNIDLKQPDIMIDSRVLVEAISYSLSSYIEEIRLVAISAIKRIYERSKIIYGEDLVFTHSLLFDLFKSFIHNCYEEAFYNKRAGVLGIKTLIDDIGITAGFLKRSQFELIDGFLFVLKDTPYEAPAIITENAEKLLIKVLQTTCTDIKELNSKISQNTLTDIVCELSNPRAKVRQACQRCLAVISETTGVPVVQLMEHSKNFLLSPIFAKPLRALPFAMQIGNVDAVTYCLGLPNTFLKFNEELYRLLQEAIVLADAEDESLSTLQRAAEYRTSEQLVQLRVACIKLLAFALKNEEFAAAQQGNIRFRILAVFFKTMLKTSPEIIDTTYHALEDVLAENSRLPKELLQNGLKPMLMNLSDHQKLTVPGLEALSKLLKLLIAYFKVEIGRKLLDHLNAWCRVEVLDTMFGQDIHEQTPTKIIVGIINIFHLLPPQADMFMNDLLLKVMLLERKLRIQLDSPFRVPLAKYLNRFHISFMEYFKKNMALRQLILFMCSIIQKPEAKDLADDFETEMDNFYEYYVSNIPTNQVRVVSFFTNMVDLFGTMAKIRGQEWLKGKREMVFKLKDMLELALRTIKRNTFYIDNLQLSQAIEKYQKIYLNFMTFYPEEKNLLLDFIEFSVSHGIEVAHPFKVFIYDNIISTKDKQQQAAYLTTSLNFALSDRFLDAGVFVLQNIVNATLIYEGSHSDSLQSLIIKGEKPDWLDLVHNKIWKSSNEILLSNVPGQYEVYRFELLQLSAIFIKWAPDMIRDIKKDVIKFNWHFLKLGDTLVKQSAYLVTSYFISQFDFPIKVVTQVFVAILRCSHAEARFLVKQSLSILAPVLHERMRTAGTPNAWTIWVKRVLFENESGQNSTLYEFLISQPDLFFESRDLFISNIIHHMSRVTFISNSNPDNHSLAIDLASLILYWEDKASKLENDAGKDQDGDINMEEGHSVDDTGKNNSNTVEDKESTPAVSMNLRETCIAFLIRYVCSSNHRASETELGLRAMKILSSLLSEKHWSNVNVKLFYFEKFLMYQDLGSENILYYSMNALDVLYVFFKNKSSDWIMENLTTIQNLLDKCIRVDHHDIQEVLQKVLVVILKAIKEKEVIAETEEETVGKTFVNTLVTIIGQDLQGTSSVAAGVMLVWTLFMVFPQSVDTLLAPIMKTFSKLCKDHLNTAQPKDATAVEEARITTRLLEKVLCLLSMKVSSLGDSRRPFLSTVALLIDRSMDQNFLRKIVSIARGWVFNNEVFPTVKEKAAILTKMLAFEVRGEPSLSKLFYEIILDLFDQKHFSNTEITVRMEQPFLVGTRVRDVNIRRRFMAILDNSLEREIKERLYYVIRDQNWEFISEYPWLNQALQLLYGAIEKDRTLVLVDAYELSSPNILAEFLPDNVSIPSESGNKDLSIFVREHISSLEECCNITAADIFEPLIEIFYQDPTAIHRTWISVFPQAYKCIPRNEKYGFVRSLITLLSKHYHSRQTNPKSNVISMLLDSISRVDSLELPPHLVKYLAISYNSWYQSIKILESIQNNTSIDNSKIVEANEDALLELYVNLKEEDMFHGLWRRKAKYTETNVALSYEQIGLWDKAQQLYEVAQVKARSGALPYSESEYALWEDNWILCAEKLQHWDILTELAKHEGFTDLLLECGWRVADWNSDREALEQSVKSVMDVPTPRRQIFETFLALQNFADTKKGDQDVRRLCDEGIQLSLYKWASLPERYSPAHKCLLHAFQQYIEFLEATQVYANLHATTAQNLDAKAQEVKRVLQAWRDRLPNIWDDVNMWNDLVTWRQHAFQVINNAYLPLIPSLQHSNSNSNVNTHAYRGYHEIAWVINRFAHVARKHNMPDVCISQLARIYTLPNIEIQEAFLKLREQAKCHYQNMNELTTGLDVISNTNLVYFGTVQKAEFFTLKGMFLSKLRAHDEANQAFATAVQIDLNLAKAWAQWGFFNDRRLSEEPNNMSFASNAISCYLQAAGLYKCAKTRKLLCRILWLISMDDASGSLANAFESFRGEVPVWYWITFIPQLLTSLSHKEANMVRQILIRIAKSYPQALHFQLRTTKEDFAVIQRQTMAVMGDRVENHNNSNNGPRQPWEHLQELNNILKTAYPLLALSLESLVAQINERFKTTTDEDLFRLINVLLIDGTFNYNRLPFPRGNPKLPANTENNLIRFSETLLAPHIRPKFNADFIDSKPEFETYIKRLRYWRRRLENKLDRAPKVEKLEKLCPHLSNFHHQKFEDIEIPGQYLLNKDNNLHFVKIARFLPTVDFVRGTHSSYRRLIIRGHDGSIHYFAVQYPSVRHSRREERMSQLFRLFNASLSKNVQTRRRNIEFTVPIAVPLSPQVRIMNDSPDFTTLHQIYDDCCAQRGIDPDSIQDFVTEQLNIAHDKALPTPDLTVIKVEIFSSIQSLFLPTTVLMDYFIALFTQFEDFWLFRKQFASNYGAFVFMTYMMMINSRTPHKIHVDKRSGDVFTLEMLPSRYPYERVKPLMKNHELNLPPDTPIFHNNEPVPFRLTPNIQKLLGDSALEGVFAVDIFAISRALIEHDNELNTYLTLFIRDEIISWFSNLHRPIVENPQLREMVQTNVDLIIRKVAQLGHFSSTPAVTTQFILDCISSAVNPRNLAKTDSNYLPWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5K0</accession>
    <name>A0A8J2X5K0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_15368g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_15368g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5K0"/>
    <dbReference type="OrthoDB" id="342264at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035361">
        <property type="term" value="C:Cul8-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990683">
        <property type="term" value="P:DNA double-strand break attachment to nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00027">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053036">
        <property type="entry name" value="CellCycle_DNARepair_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031906">
        <property type="entry name" value="RTT107_BRCT_6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47667">
        <property type="entry name" value="REGULATOR OF TY1 TRANSPOSITION PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47667:SF1">
        <property type="entry name" value="REGULATOR OF TY1 TRANSPOSITION PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16770">
        <property type="entry name" value="RTT107_BRCT_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16771">
        <property type="entry name" value="RTT107_BRCT_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="71"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="118"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="340"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="590"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="718"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="540"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="556"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="632"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="718"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="728"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="750"/>
            <end position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1128" mass="127385" checksum="1E6AD4C9130E6A30" modified="2022-05-25" version="1">MPGDSKLFEHLNFLIVVTKSEQLTAAKGIADLLRAHSCQICSIFNSYDVDVADKDVKSWFAEEVTEGNLIHFIISEDCTFPFYQVAAFDFLIPVVNSGWIFECISTKRHVRTSPFSPDPRHIFKDFQIYVSRHSFNSSEYLFYTEAVHALGGTCVDFLSSRTTHLITKDCRDPGILAVLNYGKAEPMKFVYPTWVVQSLKQFIVAPVEVHHIDPGDTIESVKAKLDELWSVVNDMSFAEPTRIWENHRFIIGMDVSLSRDLYSTFIQFLESNGGGVHRHLDEADITKTKADCYIGKSVTSKEYEVAEEATLYLGNIIWIFYMWSFGSFLLPSTKLIFSPFKRRIFETNQLIMAYTNFFGQQRFYIQRLVNCLGGYTTSELSRKNTHLLCRFPFGKKYETAKKWDKCIITNHLWVEECYMQAQRLDPLKPEFQQIPVKGGLKTTLGQIQWNEPDQSQEEINSKKADDNRSDLPMTSQPNEKKDSTDSATKLVCENISNENLIEKPKGDITSQMQETVTMRSVSRGANCKETKPGFISTKSEVLEAEGKPTTKDLNEEQSPSDSHYQQAEVSANCGELVIPVQLADLEVASPSQGNKVGSPPGHDESHQQEQSSELSQKPKSEENIETVGINLKEGHNSNKEKEAEGCDNKQEAGIVHLKPDISKLRLLQTESALNNTPARKLLELSESQKKQNFISAEQQKALEKEQTTAAFPHIHYAERQYRNRDTSAETNNTNPYSETSTALSEEDSKLTNLNHAHETSNTNAPGTGQSSEQANEETQSKSETPTTTLSPASPENASSSQGSRRAAKAKAARKLHEDIEELNEFQRNSKRKRTGNLLPEEIAQLEKRKKLENLAKETLAKVLSEEHEIVHDHANTEKSQTPSQHTPRKRLPYHINAVHTGGHNDINELDLVLLRLIGITIHNDITNENIAKLNTVIAPKKLRTAKFLKSLSFHPLKYALTPTFLKDILRIIHKNKRADLQLDLSKYYIPDMDQEKLYKFTSLPTKVFERAGILTVNIANDIPGGIEVISSILKVHGVKETKAIPTTHMNKLSSQELLANHDENGIQGGGAAEYLFLATKASQVKSLKKLAKDLNKSILAVEWDWFVASMFQLNVNFNDRKHVIYWAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5P9</accession>
    <name>A0A8J2X5P9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BN860_17920g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_17920g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFB1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5P9"/>
    <dbReference type="OrthoDB" id="360521at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13229">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3970.10">
        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027079">
        <property type="entry name" value="Tfb1/GTF2H1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013876">
        <property type="entry name" value="TFIIH_BTF_p62_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12856">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08567">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BSD" evidence="8">
        <location>
            <begin position="164"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="BSD" evidence="8">
        <location>
            <begin position="227"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="118"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="603"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="605"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF87940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="622" mass="70790" checksum="FDD9297A26493DAF" modified="2022-05-25" version="1">MSHSGAATFQKTSGIIAINEDVSPAELTWRSTDGDKTHIIVLNTIDKLQTTPPKSEKMMLRLLGKIDETKKQKDNEGNEVLPKPVTHLFSFNNRTVMDNIKITLQHIISRYKDDDIYEQKRRKEEESSGGTRNPSETPPPASLINTTTLDDSLSRGKLLSNLKLQQSLLRENKPLMRIFQEAVINAGLPPNEFWSTRIPMLRAYALSISQKLGPYNVLSTIKPVASSDNKVNVNISREKIMSIFENYPIVKKAYTDNVPKNFKEPEFWARFFSSKLFRKLRGEKIMQHDRGDVIIDRYLTLDQEYDRKDDSALLHPVQKIIDLEGNDQDDPVLKGNKPDFTMQAGSDANGHNDGSVDILKGMNRLSEKMVMSLEREYSKSKLNQEDMDREERSELVLSDLEEEYQEDYAMIHLKNQGERASLETKDSIEKSKEKHKKINKGRIHDQINLVLLSLGKELDLTQVSENSAYNNEVNQKVIAAVKTNAKQAKHNNTDTMWGVQGGGKSEEPEAKVQLPLDLLESCRMLHSTCCEFLKHFYLHFYSNKQKKQNMTKKLYKNLKECAEKILGLLDDVQKGDGDSMESICKSCLKPVLDSIHLAVQKFEESSQPTGTTTEKTIPQRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X659</accession>
    <name>A0A8J2X659_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BN860_12222g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_12222g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X659"/>
    <dbReference type="OrthoDB" id="6509908at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032218">
        <property type="term" value="P:riboflavin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF295">
        <property type="entry name" value="TRANSPORTER MCH4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="277"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="333"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="367"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="424"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="462"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="493"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="117"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF87689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="524" mass="56854" checksum="34170315C26963C5" modified="2022-05-25" version="1">MLPKAAIIGPVKALFRKKHGGYSVNDVEMQEHPKTEYISSGYGGNLSPEDSILKKNNGEVNISSVNFDNVISRDVTFENVTSKDVSFGNVTSKDVSFADTKISDSEQEDEFPDGGLDAWLAVLGAFIGLIPVFGMINSMGAIESYISKNQLKDVSSSNVSWIFSLYLAISFLSCILSGGYFDRNGSTKPMIAGTLIYVGGFFGMANCKKMYQFVLAFSVLSGTGTGVLMTPLVSVVATWFDKRRGIACSIATMGGSVGGIVFPIALRKLYNEVGFPWAIRIMAFLFLGCLLIAMKLAREREKPIVEPFQSTKEMVQWYASASLNWKYFLDTKFLFAALAASFAESSLTASSTFMASYSLARGNSQSVSYSLITANNALGILGRYVPAYLADKFLGRFNVSIITVAMAAFFNFVIWLPFGGHIGALWAYTCLYGFTTGSILSLTPVCIGQISSTQDFGKRYSTAYFLQALITLPVLPIGGAIVGKGSIINYNKFIVFVSVLMAAGASCYYISRSLCVGLRLNAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X683</accession>
    <name>A0A8J2X683_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BN860_09010g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_09010g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X683"/>
    <dbReference type="OrthoDB" id="21225at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000146">
        <property type="entry name" value="Putative two-component response regulator SSK1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="528"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="135"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="389"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="470"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="693"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="143"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="290"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="493"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="3">
        <location>
            <position position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF88386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="737" mass="81479" checksum="37C9ED51289065C9" modified="2022-05-25" version="1">MSESSTLKLRPTSPLSVNRSVPLNSALSWKIWLKLEDEAEAINQPFSLTFGEFDTVDDLKTKIFDSLNTIRWSRTNDNASIALGFYCDKSAWDPLVTEGFSPIQRLPVSPVDAIFGKGKAVTILQQQGVTARKSLAEGDSKGLSLNTSPRISASTSPKLSAMPRVPSARYGTNSSYLSVQGGNNNARSYGLIPPMSPLPFNGYSWPKKNLKHTIEQINESLFRVMFEPDEVVLNIYNEMFGHMGSQAASEALLVFCSDIVRQPTPLPFTAMSLQTHSKSDLVPESFKSQPQKEEGGEDGQKQVDVFSQPGLETPQQIAILNPEDAPPELLLEHTASQLAREENEREFQLITNEEQLRKVSQSMQDEDNPDSPKQAILLLPKDFDGYVNFNESPSKLSSQPPSPSASNTDASAGTSNVIDHQNFFHTNIENGGGYAQLRPGVQRPRTEDTLMQSHAPLSEKEVGMVHPMLESDRKKGLSSPTSPLLPRNPPPNFNRLSKSFPPKDSPSSEKNFPGDWSATSDKVFPKINVLIVEDNVINQTILSSFLRKHKIFYKVAKNGQEAIDIWKQGNIHLIFMDLQLPVLSGIDAAKKIRELERQHGIGIQKGKKVVSGELKKLNDDQINAPVIIVAFTASKSQSDKREALISGCNDYLTKPVNLHWLSNKINEWGCMQALINFDSWKHGQSRMTDNVLVKAPSHKSVKNSGNADAKSKLNRRSSDTSQSNVKEGSHLAPHPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6I8</accession>
    <name>A0A8J2X6I8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="13">BN860_10154g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_10154g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6I8"/>
    <dbReference type="OrthoDB" id="64767at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18024">
        <property type="entry name" value="DEXHc_Mtr4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13154">
        <property type="entry name" value="KOW_Mtr4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000083">
        <property type="entry name" value="ATP-dependent RNA helicase DOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000141">
        <property type="entry name" value="ATP-dependent RNA helicase DOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.300:FF:000001">
        <property type="entry name" value="Mtr4 exosome RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.30:FF:000002">
        <property type="entry name" value="superkiller viralicidic activity 2-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1500.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025696">
        <property type="entry name" value="Beta-barrel_MTR4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048392">
        <property type="entry name" value="MTR4-like_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016438">
        <property type="entry name" value="SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012961">
        <property type="entry name" value="Ski2/MTR4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF7">
        <property type="entry name" value="EXOSOME RNA HELICASE MTR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08148">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21408">
        <property type="entry name" value="MTR4-like_stalk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13234">
        <property type="entry name" value="MTR4_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005198">
        <property type="entry name" value="Antiviral_helicase_SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01142">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="Prismane protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="149"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="384"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="352"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="805"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF88434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1064" mass="120944" checksum="5A8E1D259F9FB832" modified="2022-05-25" version="1">MSSEDLFGVFNEEPAKLPEISATSLEDNDSKKGQQDRKHQLEGNEVESKTNKKFRQSKSSGEGKKKELVPVVTDAFEQEASREVDPSAGLMNTVSDQLEQDGKIKLMHQVRHQVALPPNYDYKPISDHKRLNEARTYPFKLDPFQDTAISCIDRGESVLVSAHTSAGKTVVAEYAIAQSLREKQRVIYTSPIKALSNQKYRELLADFGDVGLMTGDITINPDAGCLVMTTEILRSMLYRGSEVMREVAWVIFDEVHYMRDKERGVVWEETIILLPDKVHYVFLSATIPNAMEFAEWICKIHVQPCHIVYTDFRPTPLQHYLFPAHGDGIYLVVDEKSTFREENFQKAMASIGNQSGEDSGAVDGKGKKGQSFKGGASKGDSKGDIYKIVKMIWKRRYNPVIIFSFSKRDCEELALKMSKLDFNNDDEKDALTKIFNNAVALLPEADRELPQIIHLLPLLKRGIGIHHSGLLPILKEVIEILFQEGFLKVLFATETFSIGLNMPAKTVVFTSVRKWDGQQFRWVSGGEYIQMSGRAGRRGLDDRGIVIMMIDEKMEPQVAKGMVKGQADRLDSAFHLGYNMILNLMRVEGISPEFMLENSFYQFQNVISVPIMEKKLAELKKEVDSIHVDDEDNVKEYYEVRQMLEKYNEDVRLVITHPANILSFLQPGRLIQVVIDGKHSYGWGAVVDFVKRVNRRNPTAVYSDHESYIVNVVVNTMYADSPVNLIKPYNPSFPEGIRPAMKGEKTMCTIIPITLESIRTVGNLRLFMPKDVKASGQKETVGKSLAEVQRRFEDGIPLIDPIKNMKIEDEEFKKLLKKIEVLESKLFSNPLSHSMRLKELYEKYSKKNALHNDIKNLKNKIGESQAVIQLDDLRRRKRVLRRLGFCTQNDVIELKGRVACDISSGDELLLTELIFNGNFNELTPPQAAALLSCFAFQERCKEAPRLKPELAEPLKAMREAAAKIAKIMKDSKLEIVEKDYVESFRHELMEVVYEWCKGANFTQICKMTDVYEGSLIRMFKRLEELVKELIDVSNTIGNAALREKMETVSSLIHRDIVSAGSLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6M7</accession>
    <name>A0A8J2X6M7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">U5 small nuclear ribonucleoprotein 200 kDa helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_11584g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18021">
        <property type="entry name" value="DEXHc_Brr2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003287">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein 200 kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000024">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000004">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000001">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000062">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000013">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041094">
        <property type="entry name" value="Brr2_helicase_PWI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048863">
        <property type="entry name" value="BRR2_plug"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21188">
        <property type="entry name" value="BRR2_plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18149">
        <property type="entry name" value="Helicase_PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039073">
        <property type="entry name" value="BRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="492"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="709"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="1340"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="31"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="220"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF88495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2137" mass="243364" checksum="055A000FC7D69A34" modified="2022-05-25" version="1">MTENDGRRQKIKELYRYNEMSNKVLAADKRLLDNNGDPIKDAEESQPKSMKGRVSVKDMGSKICHDLSPEEKDEIQKEIASKLQKRSPKYPPQSTTILDSSYPLKYYPSDTENTQIYEEILVWVSDLLGSDMPHDVIVETADLLIAALKNSEKDRDGRIDRLRENLQNDLGIEVTELKFQELLKLTKQITDYESKSITDQQKAVAILSEEPYEAEREESVIETEGGEEDGIEAEEEKTHHVDREFATQGTDETLIIQGVNDNPVVKVAIQDVDEFFLRRVLANAIKNAEPTDIQKLADTIFIELEKQQDRKKLEGQLMKLLDFENLFLVDFFLRERNSLVWGIRLARVPETSKEELLKQMCDQGLDELVNQYRQRGKLHLKRQHPDELEGPDEDLNRSKRFDGENKIPPLVDLDSIKFDRTSRLLTVTKIQLPEGSYKKLAPHYEEIHIPAPEKKDVDFDPISISAFPQWAKNAFSSGETDTLNLIQSKLYPVTFQQDENVLLCAPTGAGKTNVAMMAILRVISHHINPKTNKLLRKNFKAIYIAPLKALVQEQVLEFQRRLAYLGIKVAELTGDSHLSRQQLLEAQVLISTPEKWDVITRKADESSFVQFVQLIIIDEIHLLHDARGPVLESIVARSLQGKTFQQPPRLVALSATLPNYQDVARFLRVPDTGLFYFDSSFRPCPLSQQYCAITEKASLKKLNAMNEACFEKTLQSVKDGHQVIIFVHSRKETGRTALWLREKFFESDNIDLIRKSDTASRKILDTESENVHDGHLKRILQVGIGIHHAGLSKSDRSLSEDLFADGLVQVLVSTATLAWGVNLPAHTVIIKGTDVYSPESGTWEQLSPQDLLQMLGRAGRPRYDTSGEGIIITDQSNVQFYLAVLNQQLPIESQFVSMMVDNLNAEVVAGNIQNRQEGVEWLTYTYLYIRMLMSPELYKVPKKDGDDNLSTYRQSLIHSALAILHEENLVVYEAENGKVEPTELGKIASYFYIKHNSMQVYDSQITQHSNQIDLLRIIALSDEFKHIAVRQEERQELRELLERVPIPIREDSGDALAKVNVLLQSYISHLKFDGFALNADMIFITQNAGRLFSAIYELCLKKNWSNVTKILLNFRKAVERRMWVANSPLRQFKSCPSEVIKRTEASTLPWNFYLDLQSPAEVGQSIRSEKHGKLVYDLLRRFPRLSLKCAIQPITPSLLKFELEVLPEWIWDNKWHDNIESFIVILEDTDGEQILYSDVLLIKKEYIGLEHFMDIYLTLNPSQQRNLPPNFFVTVISEKWCHSGSQIAIGLEPIRLPKKFPAPAPLMDISLIPVTNLENADFSRTFYFKTFNKFQSQVFEPLYNSNENIFIGATKGSGKTVMAELALLHHWSYSGGRAVYISPHEVKIDQLLNNWNNRFSGVAGGKVIRKLGSDMNLNFRIIAQSHLILATPEQFELVSRRWRQRKNIQRIELVIYDDIHEISRGMFGAVYETLISRMTFISTQLEKTTRIVALGSCLANGRDLGDWIGAPRNNIFNFSPQERPNALEIHLHTFEIMPSTALNIPMAKYTFKFATELQKQGVIVFLPSRRTCLTASSTFIQYAQEANWNMAKTETADLGSHLTAVDDKELKRSLTYGIGLIYEEMTSGDREITQKLFECGAISILIVSKDCCYDCPAANAVVVLGTQYYEGKEHRFVDYSANEILEMVGSAQSDGIDGMAKSLILTSPKMKEYYKKFLAEALPIESFMYFHLNDALSSEISTAVIQSKQDCVDWIAYTYFFRRIHANPSFYGVKDVSPHGISAYLTELVENALSELQRCDFIELHETDQDEIVQEEVISPLNACLISAHHNVSFLTMYMLLNSLSGSSTLREMLQSLSKASEFEGIALRQEDESILLKLNKQMPIKLTPSSGGSPVSYKVFLLLQAYFSRFPLSKELQFDLRIILRKAIPLINAIIDILSSDGLLNATTGMDISQMLVQGVWDTENPLKQIPFFDGSILEKCKEKGVETVYDVMALDDDERQDIMTMENHKLTEVAQFINNFPNVELEYSLDLTVPLAVGTPKEINVTLTRDEVPETLEVTSENFPGDKLESWWIVIGEISSKQLYAIKKVSLNKEKQTYALEFAVEASGKHQLTLWCVCDSYLDADKEVSFEVEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X7E9</accession>
    <name>A0A8J2X7E9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BN860_13344g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_13344g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in DNA replication and cell separation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the SDS23 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X7E9"/>
    <dbReference type="OrthoDB" id="449052at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030071">
        <property type="term" value="P:regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000920">
        <property type="term" value="P:septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000035">
        <property type="entry name" value="Protein SDS23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000043">
        <property type="entry name" value="Sds23p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016711">
        <property type="entry name" value="Ssd23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF36">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018148">
        <property type="entry name" value="UCP018148_CBS_YBR214w"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0129">CBS domain</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="9">
        <location>
            <begin position="247"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="9">
        <location>
            <begin position="332"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="447"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="540"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="44"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="482"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="498"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR018148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF88571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="560" mass="61647" checksum="DA042CF5A0FFB57E" modified="2022-05-25" version="1">MSSDQVSANSTPSTIGNNGAGPHGGSVSRHASIVEMLSTPPQLPQQHQHLSPQLQPQQEQQQQDQQAQPSQQQQRLRRLSNAQPANPQTELRGGNPQANTLTGANTAGLHSQIASTTSSSLNSVSSLGKDSDFESVKMTPTTTQCLNSLHSQKWQHTELSQLVEQNKLIFVSASASVEEAFNLLMKHHLTSLPVESYEGDMNCLTFDFNDLNSYLLLALNKIQVQNKEIQQDCQNGRPVPVGEMVKLTPKNPFYKLPETDNLSHVMSILGSGVHRVAITNPEMTKIKGILSQRRLIRYLWENARSFQNLEHLFNSSLQNLKIGVLNSHTKPTSRQSRVISIQGDEPLIMALYKMHVERISSIAVVDQQDNLIGNISVTDVKHVTRTSQYPLLHKSCRHFISVILNVRGLETGKDSFPIFHVYPTSSLARTIAKLVATKSHRVWIVQPPDLSPMEGTGSGGNPFAHSHFEDAVGHHHTGGSGHSSSSVHSPIMSAIQDPPSPQSSAPTPPTSSLFEKEYRTGKLIGVVSLTDILSLLARSQTEHKQVDPQSARRQRGSIYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X7H5</accession>
    <name>A0A8J2X7H5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Inositol-1-monophosphatase</fullName>
            <ecNumber evidence="10">3.1.3.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_10990g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>a myo-inositol phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84139"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="10"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X7H5"/>
    <dbReference type="OrthoDB" id="10254945at2759"/>
    <dbReference type="UniPathway" id="UPA00823">
        <property type="reaction ID" value="UER00788"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071545">
        <property type="term" value="P:inositol phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01639">
        <property type="entry name" value="IMPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000013">
        <property type="entry name" value="Inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000012">
        <property type="entry name" value="Inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033942">
        <property type="entry name" value="IMPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854:SF4">
        <property type="entry name" value="INOSITOL-1-MONOPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0452">Lithium</keyword>
    <keyword evidence="8 9 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF89177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="292" mass="32136" checksum="9CCFD3DE29DA55F7" modified="2022-05-25" version="1">MALSAEELKEVEASLIELAKTEVGPLIKSQSGTKFESYEDKANNVDLVTVVDKKVEGIVRDFLKKKYPNFDFIGEESYVKGETTIGQKPTFIVDPIDGTTNFIHGYPYSCTSLGLSENGKPVVGVVHNPHLNQTFHASKGNGAFLNSERINVSPRPLVLQKSIIGLESGSERDETPGSNFENKLHTIKNLLSEKGGFIHGSRSVGSSAMNLCYVASGLLDAYWEGGPWAWDVCAGWCILEECGGTMVSGNPGEWEIPLDNRCYLAVRGGAKQEEQKWFIESLWSHVEGRLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X802</accession>
    <name>A0A8J2X802_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S03-07756g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_07756g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X802"/>
    <dbReference type="OrthoDB" id="5430106at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018857">
        <property type="entry name" value="TORC1_cplx_su_TCO89"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22794">
        <property type="entry name" value="THAP DOMAIN PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22794:SF2">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10452">
        <property type="entry name" value="TCO89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="308"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="406"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF89039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="707" mass="78949" checksum="923AEFC2FCBC8555" modified="2022-05-25" version="1">MVQRNKSHRSELEAVPQQLSSKGSQNMPNTTGHATRPCRQFSTRSRAKSIASFKGFHRILPIDVGVSDVENSHHFGDHSFRKSRSSDSLYRKRAISGLNMTALTRVKSNPSHIIASSRPSSVNSSTQSPGAGSTGLKPSTSRSRHSVIDLHDGDEVYDDDTTTDEEVEYFSDEEDDDGGKSQKSSPSDEISKQSPVHIHRTPQETDMLSKSFTELTLPYQKPDGEPPIVNQPSYKAPSSLIKPRHATEQLLENNYNEKIDAGKHNFSALQEDGTAGASDKIDRIDNPNLTIMETGAHSLKRQGKNFAGTTEESTSETSNGENNYLSQRRNSENQEYIPNMILSQSTGVERRFDRPTSLQNSFGNNYQPSQVPQEVHRDENYSIAVNKENQFASSPNVSADQHLPRQELQQPQHQQQPHQQQNNTLLQQSNNGKGNTQHNFSTSISSLTNSLQRMAPENSYSASRKSHLTRRKPSQISFSGSILRAASGDLAVSTPPSSDINNFAQFLKSDNMDGESRTQRKLWLQRENSIMDLTSQHDSGDSIFMASNIEVKREFERISHEYTNVRRFSNPIEEALSRVESEQKMLGIETSDSHGSDALDVIFSNPVNGAKEMDQLLSHSHNTDLHNVLQSIWKEESALFNKDTNPVNPNKSPTSAHSANATTRTSLKNVMNPGAALQHQRMINSLQPTTRAVNRRIENAMHQQQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X848</accession>
    <name>A0A8J2X848_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S03-11386g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_11386g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X848"/>
    <dbReference type="OrthoDB" id="4034449at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070823">
        <property type="term" value="C:HDA1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038609">
        <property type="entry name" value="HDA1_su2/3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021006">
        <property type="entry name" value="Hda2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026216">
        <property type="entry name" value="HDA3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11496">
        <property type="entry name" value="HDA2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02093">
        <property type="entry name" value="HDA1SUBUNIT3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="171"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="598"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="442"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="528"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="184"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="603"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="631" mass="73006" checksum="6743C97723083B65" modified="2022-05-25" version="1">MNHNTRKLHVFYLPVGLTPFQRDLTEILVSLHARSFQKELESDGDDSDAMVQYPQISTKQLTYMLDKGIRVVANHPCLLVDHYMPRQFLRMEPTESLVTTSDKFQKLQELLSHFIQRDREEFPDILKICIISHNVRELDILEGLVLGKRMRIKRLSGTSLYDEKHVFPMEPSDASIDPQDSKDGTPNNESSSNKYTGYSRDDYDYSLKRKMRPIGKYEDDWLFLTTTTHLLNDPELMKGYNVDYTISFDPSLDPSLPALENLKGKGKKKSPLIKLLVKDSPDHFFLENPLPEGANKYEHLKAAIKHFLLLRHITFDNLPCVNYQEVVKALLKGEVAIPSLAEMSLSDVPKDAPLFQLTMTNLETDHNTLTIDEKIFDMKSYQSELNKRTLDRLHQVQKECRHRDEILARKRLSETERQNYLDETREGACNVFKKFQNTEKSIIDSEKRLERCKTESAKLDEKIASLDEEIKELKRITSLEDASQEIYQYTDRIWGFKNQLQTLLHDNSQKNQRNDTLRSNYQQKSSQAAEQAQCLKVLKNTAEQLQKDVDGPALRTQTNSLLSQEERLKNELASLKSRSKFLKSYVVEMTNYYSLKLSAGDDSLSNRPQTQNGRPAASRYRSTRSNTPAYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8G3</accession>
    <name>A0A8J2X8G3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">COQ4</name>
        <name evidence="8" type="ORF">BN860_13696g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Lyase that catalyzes the C1-decarboxylation of 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoic acid into 2-methoxy-6-(all-trans-polyprenyl)phenol during ubiquinone biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoate + H(+) = a 2-methoxy-6-(all-trans-polyprenyl)phenol + CO2</text>
            <dbReference type="Rhea" id="RHEA:81179"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10931"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="ChEBI" id="CHEBI:84443"/>
            <dbReference type="EC" id="4.1.1.130"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion inner membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the COQ4 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8G3"/>
    <dbReference type="OrthoDB" id="4249at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120539">
        <property type="term" value="F:4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03111">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007715">
        <property type="entry name" value="Coq4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027540">
        <property type="entry name" value="Coq4_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ4 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05019">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00081568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="332" mass="38286" checksum="6E076A70485B1C05" modified="2022-05-25" version="1">MFRLGVPKTRLSRQSVLLGQRRHFLVASAITLGGFVFGRKARLADAMENGELHNKNNDDEVAKKERMDARLKSLSHSRPMKPRYEGHVPLYLHEKMLLFIISGLRSYYHPEDGTNIIQLGESSAFSFVLESLKQTMLQDDTGRRILREQPNITSETLNMDRLSKMGKDTLGRTYYNWLIKEGVSPDTRAPVKYIDDPMHAFIFKRYRQCHDFYHALNGLPIIIEGEIAIKALEAANMGIPMATLGAILAPLRLKPVQRERLYDIYLPWAVKSGLSCKPLINVYWEELLEKNVQDLRSELGIYPPPDLRAIRKERLRVRKELKMKYEAYEAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8H6</accession>
    <name>A0A8J2X8H6_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Serine/threonine-protein kinase STE20</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Serine/threonine-protein kinase ste20</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">BN860_06634g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8H6"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008349">
        <property type="term" value="F:MAP kinase kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071470">
        <property type="term" value="P:cellular response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019236">
        <property type="term" value="P:response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001402">
        <property type="term" value="P:signal transduction involved in filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06614">
        <property type="entry name" value="STKc_PAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000011">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000385">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000007">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SAMKA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="20">
        <location>
            <begin position="362"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="687"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="430"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="963"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="136"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="182"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="250"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="304"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="435"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="478"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="506"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="562"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="631"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="656"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="967"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="716"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CDF90003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="988" mass="107064" checksum="A5B15487B8C4E338" modified="2022-05-25" version="1">MSNAADYREPQGPLPAHFADAKDTYDSETVNVSSEMESGKEVDSFDLPRVAGTLNVSALQEDETAEGEPRSGHLSLSEYRSMDPGRTVGVKEPPMEHSTSLDDPIQFTRVSSSSVLSGLSSSEGEKERMADATKQDTNLNDNNRNDTSLGVEDEGDETKRSISAANTTIPTLSGALEKSTLADKTNSTINTSEIEAQPNGLTHRPNDSSQRVNDSYLDSSIEKGNSSSKSGSVRTQHSSSGAARQKTETKTPTATAGSSTTPVLAHHSTAPQAISGSSPVNISKSSSFSPFSNSKKLGSKQDSARSPRSVSASSISSKYDQGKKKGGSGGSRMKGVFTSFVNNMKRTSGGEKRQSPGATVKISTPYNAKHLHHVGIDSRTGEYTGLPGEWERLLTSSGISKKEQEQNMQAVVDIVKFYQDVTESSGEDKTFKTFHVTNPSPADINSSPLKTPSTTSGNKFKTTPLSTDSVTPERSTETPKLQQYPLTTTPQTNSRHSPANGHTPDRNTGAQPTKSASMSTRESPLAASPIGGPSNDKFIPTRPAPKPPGGAKSLVSPPLHPSRSTSSPHVSLPHSGSQSSVSLPSRKPSNNKKEDQPLPPLPPPKEGHSPKENVVTSPGKKDEKNIPPIPSNSTPKSAKSKTPSREVSKKNNAALAEKKREERERRTKQLYAQLSGICAEGDPSKMYLNLTKIGQGASGGVYTAYELGTNASVAIKQMNLEKQPKKELIINEIMVMKGSKHRNIVNFIDSYLLKGDLWVIMEYMEGGSLTDVVTHCILTEGQIGAVCRETLSGLQFLHSKGVIHRDIKSDNILLSMTGEIKLTDFGFCAQINEINLKRTTMVGTPYWMAPEVVSRKEYGPKVDIWSLGIMIIEMIEGEPPYLNETPLRALYLIATNGTPKLKEPDNLSEEMQNFLEWCLKVEPEERASSAELLYDPFITDVAEENSSLAPLVKLARMKKIAEKMEHDDDDDVEEEYEEEVTKTQGSAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8Q0</accession>
    <name>A0A8J2X8Q0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Lon protease homolog, mitochondrial</fullName>
            <ecNumber evidence="11">3.4.21.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PIM1</name>
        <name evidence="17" type="ORF">BN860_07734g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homohexamer or homoheptamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12 13">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="11"/>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8Q0"/>
    <dbReference type="OrthoDB" id="2411602at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141164">
        <property type="term" value="P:mitochondrial protein quality control"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070407">
        <property type="term" value="P:oxidation-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000021">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.5270:FF:000001">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1480:FF:000003">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.40:FF:000010">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000015">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03120">
        <property type="entry name" value="lonm_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027503">
        <property type="entry name" value="Lonm_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008268">
        <property type="entry name" value="Peptidase_S16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718">
        <property type="entry name" value="LON PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718:SF2">
        <property type="entry name" value="LON PROTEASE HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01046">
        <property type="entry name" value="LON_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 11 12" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="16">
        <location>
            <begin position="181"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="15">
        <location>
            <begin position="897"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="85"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="283"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="825"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="285"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="825"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="837"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="active site" evidence="11 12">
        <location>
            <position position="989"/>
        </location>
    </feature>
    <feature type="active site" evidence="11 12">
        <location>
            <position position="1032"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="609"/>
            <end position="616"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CDF90046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1110" mass="124073" checksum="BC35348D9A34F7A6" modified="2022-05-25" version="1">MISSKTSKTVSQSLMYRLHLRSRGVFVSTASLRHASSLRSTRLPMDRISLSSNEHWQNFQAQEKNEKLQLDLKALEKIWMKQLSGLSAKSERDPEKDNREEHDGDNKEQEQEQEQDNNEIKKNENENDQNLDAENSGIKSSTAVSTGNGSQAPPSNNNDGGDGGNRSKSKKHTPPEVYPQMLALPISRRPLFPGFYKAVVISDDRVMKAIREMLDRQQPYIGAFMLKNSDSDSDVIHNTDEVYDIGVFAQITSAFPSKDEKTGTETMTALLYPHRRIKIDALIPPKDKRSHNIDTAQDVKEKPSDISKENERGGSEEALETKEENSSAGTQALATIDGEDLNPTEFLKNYDVSLVNLSNLEDEPFERKSPVINALTSEILKVFKEISQLNSMFREQIATFSASIQSATTNIFEEPARLADFAAAVSAGEEEELQEILESLNIEQRLEKSLLVLKKELMNAELQNKISKDVETKIQKRQREYYLMEQLKGIKRELGIDDGRSKMIETFKKRVEKLQLPESVRNVFDEEVQKLSTLETSMSEFGVIRNYLDWITSLPWGITSKEQYSISSAKTTLDEDHYGMNDVKDRILEFIAVGKLLGKVDGKILCFVGPPGVGKTSIGKSIARSLNRKFFSFSVGGLTDVAEIKGHRRTYIGALPGRVIQALKKCQTQNPLILIDEIDKIGHAGIHGDPSAALLEVLDPEQNSNFLDNYLDISIDLSRVLFVCTANTLDTIPRPLLDRMEVIELTGYVAEDKVKIAERYLAPSAKKAAGLENANVELTESAIRSLMKQYCRESGVRNLKKHIEKIYRKAALNIVKQLSIDDKPATGEDLKEDGSNKSHVSTSQDATTNTNDIEIEKTKDSTESMKVSDDIKINVDSSNLKDYVGPPVYTTDRLYDKTPPGVVMGLAWTSLGGCSLYVESVLEQPLQDCHHPSLERTGQLGDVMKESSRLAYSFSKMYLSTKFPENRFFEKASIHLHCPEGATPKDGPSAGVTMATSLLSLALKRSIDPTIAMTGELTLTGKVLRIGGLREKAVAAKRSGATTIIFPKDNLSDWEELPENVKEGLEPLAADWYEDVFQRVFSGLTVGEASNTWKHEFKLIDAKREKEKEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8R0</accession>
    <name>A0A8J2X8R0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BN860_18712g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_18712g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8R0"/>
    <dbReference type="OrthoDB" id="249703at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03181">
        <property type="entry name" value="GST_C_EF1Bgamma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03044">
        <property type="entry name" value="GST_N_EF1Bgamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000006">
        <property type="entry name" value="Elongation factor 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000142">
        <property type="entry name" value="Elongation factor 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1010:FF:000001">
        <property type="entry name" value="Elongation factor 1-gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1010">
        <property type="entry name" value="Translation elongation factor EF1B, gamma chain, conserved domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050802">
        <property type="entry name" value="EF-GSTs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001662">
        <property type="entry name" value="EF1B_G_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036433">
        <property type="entry name" value="EF1B_G_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00647">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01183">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89942">
        <property type="entry name" value="eEF1-gamma domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50040">
        <property type="entry name" value="EF1G_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="8">
        <location>
            <begin position="87"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="EF-1-gamma C-terminal" evidence="6">
        <location>
            <begin position="248"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="213"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="213"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF87972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="409" mass="46713" checksum="C251E8CFB8D25E83" modified="2022-05-25" version="1">MSNTLYVATFTPRALVTVGLAKFLKLDVEIVEDRESDERYTKSFPLDKVPAFIGKNGFKLTETIAIAYYLVALTGDEKLENTLVGTTAEEKANVLRWVSFANSDLVQQLIKAYFPLVGKVPYNKKQVDAALESIEKCAQFFELRLKEYTYLATENVTLADLLSFYSWSAVLGSVCGAEFRKKHPQLTRWFNTVATHPAAETAKKYFKLAEKPLEPPQGKKKEQKKEQKKEQQPKKAEQAPAPAAEKKPKHPLELLGKPALSLDEWKRKYSNEDTRPVALPWFWENYNPEEWSLWKVEYKYNDELTLTFMSNNLIGGFFARLSASIKYMFGCMVVYGENNNNGIIGAIMIRGQDYAKAFDVAPDWESYQFTKLDSSKPEDRAFVDDMWAWDKPVIINGEPREIADGKVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8V9</accession>
    <name>A0A8J2X8V9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine-protein kinase ATG1</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Autophagy-related protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Serine/threonine-protein kinase atg1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">BN860_06150g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="CDF90335.1"/>
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        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    <keyword evidence="11 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
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            <end position="315"/>
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    <sequence length="893" mass="100610" checksum="D90328E3C1B15B32" modified="2022-05-25" version="1">MTERRPTTIVNGEYTMEKEIGRGSFAMVFKGYLTHDKSKHIAIKTVSRSKLKNKKLLENLEIEIAILKKIKHPHIVGLMDCERTSTDFYLVMEYCALGDLTFLIRKRKELAEKHPLLKSVFEKYPPPSPNHNGLHRAFIVNYLQQLASALKFLRAKNLVHRDIKPQNLLLATPLVNYHDARAFHENGFVGIYNLPILKIADFGFARFLPNTSLAETLCGSPLYMAPEILNYQKYNAKADLWSVGAVLYEMCCGKPPFKASNHLELYKKIKRANNVITFPSQCVIEQGLKDLICGLLTFDPASRMGFAEFFENDLVTEDLSPYELDEAVPELEIKSKDVVESNMFISEYLSKPATKKSLGANALTKNDIKPLTPNDSSQTDMNTRKSSPNPASHPIVNSNRAMKRNPSLERAKKIGSSGSGSDIMLEKEYVVVEKKTVEVNALADEFAQAGPGPTILQHNRQSTSPSQRQRQLSDTSPSFAAGLYVGGQVQQQQQQPITAGRTTRPSNSSGGSSSRRPSLVDRRLSLNSLNPSNALSRALGIASTRLFGATPPTQQQQQQSSNASSPNYRSSLLNPKIFQDLTENIILRVDHLQGQLPQKMDGNSVVLWLETLSAKAFVVFSFAEVKFSQIVPLPRTSKDHQKRLSNSSCAIEEEDEEEPDFLTRHTLHNRSSSAVSSMLNDLPADEMHQLCTEAIILYMKTLSILAKAMQITSNWWYESQDKVYSLRLNLLVQWIRERFNESLEKADFLRLKINELKTATVSDRTLIKKEDAKAHEGGSDLQQEEESPQAGENSSEEPVYLEKMLYDRALEISKAAARLEMQGEHLNNCELAYATSLWMLRTLLDETSDEYPYNDEDIVTTGVLDEQDKKIIRKYIDSIANRLKALRQKMNHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9J1</accession>
    <name>A0A8J2X9J1_ZYGB2</name>
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        <submittedName>
            <fullName evidence="8">BN860_15148g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_15148g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
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        </subcellularLocation>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="TF_Aft1_OSM"/>
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        <property type="entry name" value="CYCLIC-AMP RESPONSE ELEMENT BINDING PROTEIN"/>
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    </dbReference>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF88648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="642" mass="69312" checksum="195D7314CE143B24" modified="2022-05-25" version="1">MSTTNKQSGVSSFDLEPNPFEQSFASTKEQHPVPSNGQQSGSSYAQQEQQPQAQQNLQQRTDSRPPLNVGRSTDGKSPFFYGSQKPNILSPPLLTPGGFRRLPPLLLSPSGVPQNTSSSGSAQATIMPSAQVGNMANSTANSGNGAPNVSNATAGSKPEINRTPSFLLNLSKTGLTPNESSLRTGLTPGILPPSQSHHYYPTLPALNSVNQGSSQAKPGSSGTAGTTPAPFTPGLGSLLGFPTNASPGQSGRTSVNAENFQHVTEAAVIPHENAQTQKVASETNRLQKKLPTHTDSPTQSQTGQKRSSSYAGKTSKNSKRSQSSTPNSVENRRASRNEESQNVEEGKDINDDDQERKRKEFLERNRVAASKFRRRKKEYIKRVEMDLKFYENEYDDMSRALDKLCGIVHGSPTPVASSSLISMLESSISQNDVPSSLSLLAHMKQVVYETRYFQRNGRNPRREIEKIQETDDEDRHRTDNDSAGNIRSRNGSTAGSTDLARDKRPSSANYPGSVPASFSSTGIQYMTPVQAMSTGSMPSFAHDGSSIHGMVKSEPNISAMLPLSIMASKNPASEQDVSSTIGTANSPSDVANGRQLIPMNDINQQVTPHNNSVPEIRHDSMADLANQPIRTEPALPKYPHTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9M5</accession>
    <name>A0A8J2X9M5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S09-01310g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_01310g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316462">
        <property type="protein sequence ID" value="CDF90946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X9M5"/>
    <dbReference type="OrthoDB" id="10379295at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="698"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="866"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="931"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="705"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="866"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="933"/>
            <end position="944"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF90946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1215" mass="137066" checksum="60907368D24F09D8" modified="2022-05-25" version="1">MRSINFTEHFESAGYIANSRTNNPSSSIADGEIVDTNSRSSVKETFKKFIRWRYDPDDCNSDSIIHDFPVAVSTASAAVGDPAHTVSPTLSKSWRDSAVFTAADLPAKKAVTSCRPHYSNEENKAMPDSDKKENKFSGHPSSKSKFIVGVRRLFYWGHGKKENELDTTDKVAIKNPMNDFFNIEPSESELGNPQVNPIRRKRDTMKLVCSNSLEHQFFLPSEVNEQNSSELDIKYFQKHRSRSFDFPESQSPEFIRMQKNAQKASRGIKRKLAAGGPSQCYARFSAKNCDSRDGRENVANTLCLKDSTNPTTPKFFSLNSAPREVPKVAAQLTLSSDERKVNELTMSAVKTNVDCWHDSPRLLSSNYQDERISISRHNNENSIISSINVSDFYPLKDPENSPYCKSVDDHEFLTEGTKPIGNLDAEHKVDTMIAPCKDYVKKSFRRTSSSDFVYDVDVHQNIEALIYAGESDKSPSEDSQTIDWHGNEVVSQKRNDTHTTAAKGNGSAIYFSGIRDTSNDARDINLIIEYRNAGNEMLHLPRHSSYDTVVHYDIPTAATTLPDDNEDFEISNLTLEKAEQKLPLSNCGIDTAFFKNFGSLFSNAKKACTGCDGEDNFKTSAAKVNTEAILTTLLDASVPYSSDRDSNRGEFAKESASQKFGTAILNNCAANISTNGNDGNNPKIAEYEINSHDEQFKNRLPTGTEKPNSPPIRNSVPSQASPVKASRIGAYRVDSQVKSVTNTRWYGISPETGSNADVEELMVPYANSRTLHGLSSFNNYWRMKDCHDSPLKTSCNTAISHESNYPPSTYEGFIHHILGVKSNEVQQLAVDHGATGNNVTEADREPDHSTCREDITEYGIIETGNNIVKGNKNSQSKYEPGQEYGPEGNEAPSFYLTSHFEARTISSLAENSLRDHDGSNEEPHEAAILGASHKGDDADTDRTNSSEGAAVDTPSPTPPPQQADRAETQETLLPLERHQCIFGSVSPDSICFVHWPIVKENVVARVLGDYCPLRFRNNIRFDYDGSDFRVKQYYMTNRAILQEQYRTRRRVLFAPRPDKWFHFEEETEILDCLDDFIKVNKCLYPMFKGPRIVQRKHMFRRWKNRGRDRLIILEQLLQYWRQDPAYIEQLKREKELRRSSPLAYPQKPERPPIKIVLQKIIDRMTQDMAHKRLGRPPRAGSSVILLKKGPPLGIDYFGYKKQRAQEWVNNLLLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9N8</accession>
    <name>A0A8J2X9N8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 18">Serine/threonine-protein kinase Tel1</fullName>
            <ecNumber evidence="4 18">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">BN860_03070g</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates histone H2A to form H2AS128ph (gamma-H2A) at sites of DNA damage, involved in the regulation of DNA damage response mechanism. Required for the control of telomere length and genome stability.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 18">Chromosome</location>
            <location evidence="2 18">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 18">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the PI3/PI4-kinase family. ATM subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4 18"/>
    <dbReference type="EMBL" id="HG316461">
        <property type="protein sequence ID" value="CDF90790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05171">
        <property type="entry name" value="PIKKc_ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000032">
        <property type="entry name" value="Serine/threonine-protein kinase TEL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038980">
        <property type="entry name" value="ATM_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044107">
        <property type="entry name" value="PIKKc_ATM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021668">
        <property type="entry name" value="TAN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11640">
        <property type="entry name" value="TAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01342">
        <property type="entry name" value="TAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13 18" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 18" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10 18" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="14 18" id="KW-0779">Telomere</keyword>
    <keyword evidence="8 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="20">
        <location>
            <begin position="1734"/>
            <end position="2328"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="19">
        <location>
            <begin position="2440"/>
            <end position="2755"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="21">
        <location>
            <begin position="2762"/>
            <end position="2794"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU365027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CDF90790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2794" mass="322318" checksum="D8A0F5486793DE28" modified="2022-05-25" version="1">MEDHGIVNVLTDLASSKVKERNQALDDLTTTLKQRPDLVPTKLLASTAETLIELVEAEKCKYCELLTSFSDSKRGKLSLSENKLSSVSYVLRLLVEKTCSRFKIKTLKLLLAALPELTVREGSHDLLEPSSVHLSYALLALVKSDLFQLKFTMQQWVSMVDTTCSLLGRRIKSSINDRNVSNFFALLSTLMSMDTVGVEYISAALARTLTQYLRLSQKENVNTRLALLLVNQLTVKAHCLNIRACLQLIKETWKHAISIAYSTNSEIQRELCYLDVFASELMCNKLPAMMGHDETENCPDFVSLSSLLHEYLTLRLANYRSTSLPIQWLRLDEKREIKVQWFELSDFQLKEKSNAHPWFNALAVTKLLSCYYFLLESKGEEKDIQLFKKRKVEPAWSSLLRDSYGVEDFICNSLDIGNSEIQLCSLQVFAFHASMQELDLEDDHRLRESLFQRFGDVEHAGWTCLCLTSLISQQGFSLEKTDLNRIFKLCLPLVKVPEKCQVACRLLSNAIKYSKLRITDTTTLNQIYDTFELSPINGPSVVCNEAFAFWQHLETYGKEVQFRLGDSTASKILEWLTSKWDQLLRIENHQNLFYDFLSWLSGRKKDCADDLNDYPFFDWRYNLWQDHYFIWQKYKDQRAFLLQKRPSSKKLKSERECNPVSSIFVERTTLNEILYRLLGIIEDFDGDASLLQFKWAVQVLKLVNRLSGDSSFLEYAADFKRTVSLVLPTMTFKDKASYIDFFQTILRLNAPNVEHIIFQGLRMKDIIISFRCTLNQVEIKQTSPMTEFDAVFDYGSDKDTNHQMASLANPYYHNDSQICVETLLRVLKGTKEPFNSSFEVLLDFLNGMESDILMKCLCPIIRWLGTIDQKEASYHRLLEQFTQLLSDHLLNKELNTSNQAIYLLCSYLDVMRPQWLVHFENSLNSDCNDILDWVISRFEENAFSGLCAITKLSRALLHMLRFNDLSRTCVKGGKQRIFATFTNCLKKLDKATILTELPEIITYMSIVSYKNQRIIFSEIKELFDVPQQSIEYSAFYVYSMLKLSSMSYSNLVFSMQDMTDYSCFEHTRTYIITALERMVEFLELQSPLDLLDISKFDMLYFWCSKNMTLNGKQENQWNIELYGFEHLEDFLCRYSSEITAFCFSKDQKSSPLMEKLCQVTKKTATQLFSESFHIALPISFVNGRAAGGIIDASQSLLGSNLMKSCSQVLLYRWILRFCDFGSLVDTGLALGKWFPNSLILKELFPSDAATPRYQLPLYIPAATWTRLLNDSLSHIKILEGDLRFLIIWILADLEVSKFGELRLACVRQLKSVMVAHEEILSECKFLPCILEMLAKFLRDSKIHEEVLSIIVCLLKLADKNNLSIVEALPSLYSEILIFIEIHQTEIHHSFKVMSASICTRDIPFSKTWHYCNDIIQGNHISDNIYENDEILKVEDTNRDRVILLSLMFSHARIPDISHLNVEPSILAVENLLKFKVPDEYISRNFSLWSAYYLDLFESAQEMSELFEKSGLAQQNMSFRDYFGDPGSLCFLFDKFFQFYEDSLPFQPCQIQLLWESTMEFLLNKYRKNDEDPMRISEFYYDKYKGRMTHMDSKLFRLVNGGPKTVFNMETFVTSHFLSSGESYGSWLQNFVGSLTDHLCLNIPDMKLFYYLSLASHSFAESIITILFNLLMYYDPKKGIYWASRMFSDIEKLLVVAEPAEKTFCVLQIISMLRSGNRINDRHCVSAYTTLPLKSVCESALKAHQVAFSYMLFEEYSMNDLGNLDTITLGTIYESLGDVDLMAGLPTSHNLVGALHSINKIEPMTWKSFLVNNANFNANYNEYLKGGQRPLLKATESQGFYGLASSLCSEMSTQYSSESYKWALQLGNWDLPLPQHIDTKYKGLYYSLRKLMHEDSEPLKCLQASLIMLAEKRSDFISQTEWVSTISSISLLQRFASSMKTVESAVATLRNLTILDNQKLEFCEFESYKSIVQAKYLLLDILSRKAENAGNHVPSEIKTVNVVQLAKDVKYAIENKSPQDALRYAFTLDGLVKQIDAVDPEFPLLLSLKRLSSFVSATALWECKEFKTPILILKDLLHQHKEQDNENSAHQHFEFLLKVPDDYIKAMLVKWTSQSRFEAPSVIFEKYIRNFDTKIKDFDLRASMFYIMGNFLSQQVQKLQASKEIEERQHRCDIQANESHALETIHKNTSLPDNERKDAKRHYNRVRLQLANDTEILNSLKIQRVQFVSKALHYFLNTLVFTNKYDEDVLDKFCGLWFEHDRDDDINILLFKEIGSVPSWKFLPWVNQIASKLSIEQTKFQKPLQLTMKRLLFKLPYDSLYSVMSILFYGKESVNLNFRVSQKVQAVEKLLKELQGFEKGTYFSKYVLPIREFCEKSVELANTKFELKSKSSKKLHLPGLIMGEYWLNCLPSQKLPLPTVHVPIRSSSDGKLARPYITSTVETVDISSSGISLPKILTFYISDGTKRKVLIKGSNDDLRQDAIMEQVFGQVNKILQTDKQMRKLDLNISTYEVIPLGPNAGIIEFVAHSISLHQILTVLHKNDSVSFDQARRSMRTVQTKSKTERIATFKNLIEEIQPQLRNFFFDYFPEPLEWLSAKKTYTKGVAASSIVGHILGLGDRHLNNILLDYKTGKPIHIDLGVAFDQGRLLPIPELVPFRLTRDMVDGFGVTGVDGLFRKSCENTYSVLRENREKVMYVLNILKWDPLYSWVMSPVRRHRHLLEEDSQLYGSLNLEEMESDAKHKEENQESFRALKGVEEKLIGNGLSIEATVQELIQQATDVENLALIYMGWSPFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XA80</accession>
    <name>A0A8J2XA80_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cobalamin-independent methionine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Methionine synthase, vitamin-B12 independent isozyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN860_02102g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the vitamin-B12 independent methionine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.14" evidence="4"/>
    <dbReference type="EMBL" id="HG316463">
        <property type="protein sequence ID" value="CDF91207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XA80"/>
    <dbReference type="OrthoDB" id="1053771at2759"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00082"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03312">
        <property type="entry name" value="CIMS_N_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.210:FF:000003">
        <property type="entry name" value="5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00172">
        <property type="entry name" value="Meth_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013215">
        <property type="entry name" value="Cbl-indep_Met_Synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006276">
        <property type="entry name" value="Cobalamin-indep_Met_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01371">
        <property type="entry name" value="met_syn_B12ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003556">
        <property type="entry name" value="PRK05222.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30519">
        <property type="entry name" value="5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08267">
        <property type="entry name" value="Meth_synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000382">
        <property type="entry name" value="MeTrfase_B12_ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Cobalamin-independent methionine synthase MetE N-terminal" evidence="17">
        <location>
            <begin position="4"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Cobalamin-independent methionine synthase MetE C-terminal/archaeal" evidence="16">
        <location>
            <begin position="439"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="705"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="444"/>
            <end position="446"/>
        </location>
        <ligand>
            <name>L-homocysteine</name>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="444"/>
            <end position="446"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="497"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="574"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="612"/>
        </location>
        <ligand>
            <name>L-homocysteine</name>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="612"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="655"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="657"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="677"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="737"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CDF91207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="767" mass="86202" checksum="2D7B0795F57D8B79" modified="2022-05-25" version="1">MVQSAILGFPRIGPNRELKKITESYWKGKASVDELLKVGKDIRAANWKLQKEAGVDIIPSNDFSYYDQVLDLSLLFNVVPERYTKYDLSQLDTLFAMGRGLQRKATETEKAVDVTALEMVKWFDSNYHYVRPTFSHSTQFKLNGQKPVDEFLEAKELGVQTRPVLLGPVSFLYLGKADKDSLDLEPLSLLDKLLPLYKEILNKLHAAGATEVQLDEPVLVLDLEKSAQDAIRKAYEYLGKQSNVPKITLATYFGTVVPNLDALKGLPVAGLHFDFVRAPCQFEKVLSVVDKNQTLSVGIVDGRNIWKNNYAKSSELVNKAIEKLGADRVIVATSSSLLHTPVDLENETKLDPEIKDWFSFATQKVKEVVVIAKNVSGADVKAELEANAKSVAARAASKRTNDEAVQKRVSSINEKMATRAAPFPQRLAEQKKVFNLPLFPTTTIGSFPQTKDIRINRNKFVKGTISAEEYEKFINSEIEAVIRFQEEVGLDVLVHGEPERNDMVQYFGEKIQGYTFTVNGWVQSYGSRYVRPPIIVGDLSRPEAMSVKESVYAQSITTKPVKGMLTGPVTCLRWSFPRDDVDQKTQALQLALALRDEVDDLEAAGIKVIQVDEPAIREGLPLRAGEERSAYLKWAAEAFRVATSGVQNKTQIHSHFCYSDLDPSHIKALDADVVSIEFSKKDDPNYIAEFKDYPNHIGLGLFDIHSPRVPSKEEFISKIRNILKTYPADKFWVNPDCGLKTRDWEETKASLTNMVAAAKYFREQYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XAP9</accession>
    <name>A0A8J2XAP9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ZYBA0S14-00760g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_00760g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316467">
        <property type="protein sequence ID" value="CDF91773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XAP9"/>
    <dbReference type="OrthoDB" id="68611at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018823">
        <property type="entry name" value="ArAE_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023244">
        <property type="entry name" value="Brefeldin_A-sensitivity_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052430">
        <property type="entry name" value="IVT-Associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049453">
        <property type="entry name" value="Memb_transporter_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47804">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47804:SF4">
        <property type="entry name" value="AFR661WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10337">
        <property type="entry name" value="ArAE_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13515">
        <property type="entry name" value="FUSC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02047">
        <property type="entry name" value="BREFELDNASP4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="613"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="664"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="696"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="721"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="750"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="domain" description="Putative ER transporter 6TM N-terminal" evidence="6">
        <location>
            <begin position="25"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Integral membrane bound transporter" evidence="7">
        <location>
            <begin position="644"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF91773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1185" mass="134012" checksum="4ADA999286E51087" modified="2022-05-25" version="1">MKSANRTQKLLRWWQRTFPCDRVLTQRIAKSVVNSTVALIFCLIPKVRKHLGAEPSMLPMISVIVHPGRRISGTIQGTIFCLTGLIFGLAYALFGRFIAQRCLGHNWKHLEESQQYALHYQRYESALAILAVFETFMLFFHGWMRSVSHHYFGIVFPLFLVVHFAFLDPLNDPRSTTAKAYTVPFYLGIAMSIFWNLVLFPEFGSTYLGNSTIDTLNMIHDTVDDSVNFFISVDMEEGQSLYSKPPCSLSKLLKMKSTIHNKVSTCSMVLNECIYEISYSYLSPTQLKPIINIFKSLAMYINGLANSCQLEFTLLARDNMREGDIMHINTDKEITYADFEQFDKVLCKLRPSIYNLHKILSESIYIAKVILSNAYDVKLSRVKCPEIFGDDGTTRYKSKKDLPTDFDIDQHRNSLYNALIDFDVQFREEMIGVDNDFLSPRDEMFLLSSFLMNFKESTKSVITILDLVKEIHAARKSRESKGWLRGKSIWFTCLNSFQTFMIWFKGSYSKINSVNENQTLKGGAYAPENQLMAFLATRRPSVEEHDLLSQQLESSKDLKDFATPAELPLPAVASSNSNPSNPIKAKNWRSKGLSYYFIIAIIKANRFCRRSKAHFRFGFQVAVALMLASFPMFVPKTRPWYIRFRGAWVGFVCILCMEPSVGGTFWAFFLRMLGVIIGSAWAYLSYAAGHHQTDPYLETVITAIGAVPGFYFFLGTPYVRAAIIGIISIYIVLLAAVLPSSVHDTILTSFAKRCFAVGYGGGVALIVQVIFFPSKARDELNKEIAFICGSISELELLYATGLEGESLKTSLTQERYDKMTYISQSAKAALSRADAYKGLARQEPRLKGEYTELEKVFTQIIFVLRQIVERMDNASLLRKQYGSAVIEELNSVAYPYRRQLVGSICCLMRAMQEAFLNKTPLPQFLPSARIAHRRLINMVRRALKNRYRGRLSALQSKSPSKLIHDDDSDEEQEIMINVKRRNREADSQGMLAPHEFALKERFLSWSASSAAMEEIIEYVEELCNLTKILVGINEFKYGFLSRPLYEDWAAEAATGFDNFIQGKRARKEGGLRSGEPAISEYSELSGSSVSDVVQDPVALHPEEPGVPLNLARIASHKAGHNGDDLPAPFRKRSFSIGSLGGQLGLWRSKTLGDTDSDLDVADDTSDEELPLALKRIVTGDRRNPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XAV5</accession>
    <name>A0A8J2XAV5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ZYBA0S16-00606g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_00606g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316469">
        <property type="protein sequence ID" value="CDF91944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="343070at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057974">
        <property type="entry name" value="NUA/TPR/MLP1-2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012929">
        <property type="entry name" value="Nucleoprot-TPR/MLP1-2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18898:SF2">
        <property type="entry name" value="NUCLEOPROTEIN TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18898">
        <property type="entry name" value="NUCLEOPROTEIN TPR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25785">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07926">
        <property type="entry name" value="TPR_MLP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NUA/TPR/MLP1-2-like" evidence="7">
        <location>
            <begin position="471"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoprotein TPR/MLP1-2" evidence="6">
        <location>
            <begin position="1018"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1576"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1639"/>
            <end position="1687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1704"/>
            <end position="1815"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="76"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="294"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="552"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="700"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="941"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1165"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1424"/>
            <end position="1465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1591"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1676"/>
            <end position="1686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1715"/>
            <end position="1727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1738"/>
            <end position="1752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1764"/>
            <end position="1776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1777"/>
            <end position="1788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1805"/>
            <end position="1815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF91944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1815" mass="209183" checksum="7283E46579EDBF19" modified="2022-05-25" version="1">MPGSVSRAADAAEGTNDNNGNLSSFYNFSEEQVSSIDHSILQSLEKKVEDFRELQSQNLKLNVTIDEMKYLSTQRADELKAQIGELMKENDSIRAERSHIEEESRQLSRDKLNAQHDIETLEEKLSILTQERDSLKVSRQEVTVLLEEKVTELGSCREECQKTVQESKDIRRQMLELENTVQNLKSREIRDQSEIQTMKQQMAIMKKNTEWLEKEVTTKTEQLATCRHKTEDEIQRLTTDSMTAKNDLQMERSRNDVIAAKNNELSKALQAKLLEFKEMTDSLQAQKQEFVHEMAMKQKLIDLLESQVKSLQNELTASLDKNDSDLHAQGEVSADGEKRIQELLELRNKFEESEQERLRLELVVKELLPEKKEIDPQDSNSSFASLRDKDGPLIKTYGDVGVLKKELIKERRQKERLQRQVESFIVELEYKIPVINSFKERTSMLEKELNDVALLLEHTSNEKEKKDREFEASSKKIKDYESTIQILTKQRTDLAHQVQFLLINLSIHGDSRGPLSPDETAFVKRIISNDDPSSQTDSQRVISERLVEFNNIASLQEKNMELLKIVRKLADRLEAEEKDVNKKIQHFENDTIREAKEAIVTLQDYNSSLESRIDVLTKERDAFKALCSTDDESTARQTNSGKVNSAQYTGEEAVRALEARLTSVTVESSQNNKMLNEEIQELYKSKTQISIELQKERSSKTLAEERLKLIQHSLELARNENSELIKRSQNLQSILERQDTRTTETVNELIASNSKLAVLQTKISNLEAERELLQSSNEASKENNLKLSEERNSLRIMVTQLQTLQSEREKFLHEIQKAYRENLDSLESEKAELKARMNSKIRESEEVESNKRVQIQWYQDKVDSMNTTARDLKQEIHDKTLLVDSLQVEIKKLQKQQEESEARIQSYKLLNDSGNEDTTQISLRKELEKTNIALSEACAEIEHYKELLSVNQDSLDQLTQEFNKQKNHFVLRVEALEDQKADLEHNIADLNGQLSNLNGTLQDTTEMAEKEKINLMKKISMLETSNKGTEQLKEEYDAKISQLQRDLEQQVSFANRAQRNYEEELQKDSNVSKQISELREQSQKDKLEIEKLKTLEEQAKRVLEQNEKSWLAQKEEYEKQIDTSRQHLEDLSAQNGLLYDQVELFSKDKGGDIVSGENSEIREILVNLRRERDILSTKLAVSQREEQLLRQSLATNADELDRTKRQLSQFQREVSAHSELIVHHEQIMQQLDQLNLLRESNITLRNVSEEANKRNQELQEELDRLLAKILPLESELDGLQKTQLEKNQQVEFYQAEVNRWKERSQEILRKHDRIDPEDHRLLNEKVKILEAKLEETSKENKELDDRFSRLKKQAHEKLNNSKISQASLTAQINELKEAKSELERKFEEEKKKVHELQEKVSIQIADDQVVDAVRGELADSLEKSKGLEQKVQENDSLISKLREEIDVLKNQLNALKDKNATMKSGNIPVNMANIVESMKQSFEEEKINFIKEKTEEFRRLEKQKDTAQANGRNVPSNVNYEEMKKQLESEYEEATLKRIAEAEDNLKKRIRLPTEEKIKKIVEKKKAALEEQYKKKLEESKLSVDSSASDPSERESLKQQIEKDIRAKFEEELKAVKKKAYEEGRQQAAMKSTLLERKITKLESQLQNGAESPEKDSNGMPSTKRALPSKIDEKSITSNKNVSNPLVSGEKVLKLDSSKPAFSFQALGGSNPFTSSPQSSDASSASAFGFKPTFMLSENEKKDTNTSDDQSKGVKPINPFSSSTDEKTNYPEHDSSKTTTPISNQIVSTPLKRAVEDEGDPEAASENKRPKDQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XBA5</accession>
    <name>A0A8J2XBA5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ZYBA0S05-07822g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_07822g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XBA5"/>
    <dbReference type="OrthoDB" id="6093671at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016428">
        <property type="term" value="F:tRNA (cytidine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057285">
        <property type="entry name" value="Pre-PUA_NSUN2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057286">
        <property type="entry name" value="PUA_NSUN2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023270">
        <property type="entry name" value="RCMT_NCL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22808">
        <property type="entry name" value="NCL1 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22808:SF1">
        <property type="entry name" value="RNA CYTOSINE-C(5)-METHYLTRANSFERASE NSUN2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25376">
        <property type="entry name" value="Pre-PUA_NSUN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25378">
        <property type="entry name" value="PUA_NSUN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02011">
        <property type="entry name" value="RCMTNCL1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="12">
        <location>
            <begin position="52"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="416"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="671"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="416"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="447"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="466"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="674"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="170"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF90050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="709" mass="80225" checksum="F8FE9201E1401841" modified="2022-05-25" version="1">MGRRNFKKGKTFGARNDRDNGKGWSELVRENAKWEEYYKQLSLFPADQWDKFKSTCQAALPLTFRITGSKSHAQEILALFKERHLPNLTGVEFEGETLKAPMELPWYPAHLGWQLDVPKTVIRKNVQFAKVQRFLVVENAVGNISRQEAVSMIPPILLEVEPHHAVLDMCAAPGSKTAQMIEALHRDNDEPSGFVVANDADAKRSHMLVHQLKRLNSANLIVVNHDAQFFPRIKANSSQKNRKDILRFDRVLCDVPCSGDGTMRKNVNVWRDWSPQNGLALHVVQFNILNRGLHLLSPRGRLVYSTCSMNPIENEAVVAEALRKWGSQVKLVDCSEKLPGLVRSQGINQWPVVDRNMQVKERGEEGTIESWFPPTEEEASRFHLENCVRVYPHQQNTGGFFITVFEKAGDLGSHKSVASAQESASKTENDEEGAEAQEPAAKKLKTDVNSNTSPAATTPTESVPTPEKKERLPRDANEEPFTFVDPNNEAMKNCWKFYGVDDKFDKNTCLVRNATGDPTRVVYTVCPALRDLIQANDERLKIIYSGVRLFVSQKSELECPWRIQSEALPIMKHHMTSNRVIKTNEEILKILLEDSFPNLDAIADSNVDSQFVQRTRDISSGCAFIEVIRDGPNKENLFLPVWKGKKCINLMVCKEDTHELLYRLFGVENVPKNGKNDVENKKSDDSKETDESKVDVVKEAPPQETNEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XBG9</accession>
    <name>A0A8J2XBG9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ZYBA0S26-00100g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_00100g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316479">
        <property type="protein sequence ID" value="CDF92096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XBG9"/>
    <dbReference type="OrthoDB" id="40134at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF461">
        <property type="entry name" value="AMINO ACID TRANSPORTER TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="401"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="469"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="543"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="118"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF92096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="584" mass="64427" checksum="5C514571D4DCB8A2" modified="2022-05-25" version="1">MSKLVPIASHSLSLNTSKAEEEGEKENVLQTASDHEKGEFIDVSGREIISDENDKKAHQLPLQAYTYYADIQREYERSKDYDGLYAAQREQLYTDSNSNLSPSKLDSQRMMRIAKAYSVFFLITTDILGPMNAPNAVSQMGYVPGVILYVLFGVAAAFGGWLLNHCFCKVDSNNYPVRTFSDLASRVVAPWFRYPFGLLQFIQMILNCGLLLLSTAQAVSQMLVVNRGKDRFCFTVDILVWALLAMIAGQISSLGKFAHIANLSVWMNIAVCIITMVGVAVGGPYYGIFQQYGQISPYFEPGTWNPMPITQYAIAPGTIGDKIGGMNNMVFAWGGATIFCEVMAEMRRPMDFWKGMLCAQSLILVIYLFYGLFVYSYNGQFCYVTANQAIGSKALQNAGNVLNIISGIIAMVLYGNVGIKVIYQGFLVADFGFPSLTSRKGTFMWGGFVIVYWGIAYILGAAIPSIGALVSIIGAFCILNFSYTFPFLFGFCLLCRQDAATADEFDLKTLTVVQADSYLRNWSRWKRAIGYGGAFRVFVKISLFFLFLASLATCGLCSYSAIVGAIADYATNTAQPFTCNSSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XDW9</accession>
    <name>A0A8J2XDW9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ZYBA0S12-00364g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_00364g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316465">
        <property type="protein sequence ID" value="CDF91501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XDW9"/>
    <dbReference type="OrthoDB" id="18234at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040185">
        <property type="entry name" value="Far11/STRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021819">
        <property type="entry name" value="Far11/STRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012486">
        <property type="entry name" value="Far11/STRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13239:SF4">
        <property type="entry name" value="AT25231P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13239">
        <property type="entry name" value="PROTEIN REQUIRED FOR HYPHAL ANASTOMOSIS HAM-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11882">
        <property type="entry name" value="DUF3402"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07923">
        <property type="entry name" value="N1221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01293">
        <property type="entry name" value="DUF3402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01292">
        <property type="entry name" value="N1221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Far11/STRP N-terminal" evidence="2">
        <location>
            <begin position="203"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Far11/STRP C-terminal" evidence="3">
        <location>
            <begin position="567"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF91501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="933" mass="108216" checksum="0F2A72359C372C89" modified="2022-05-25" version="1">MNRNGRPDLPIRSVSLDNLRPKSNLRKRLKFIDDTPTRSSSSASGSPERDRMEDDELVLDLDNILRTKLRMTQSLVDSERRGDTSHENELAFKAKSGLEGNVMTTYDCDEENGINDAQLNQVTLQGDEQKSDEMGDEDEEDEDEEFANVDEIDGPISGKKNENLEDGHGEVDYNMPVDEAYKKSLDERAAEFDKVSHFQPISQPRVEWELMNFYTLRDDLEEWFCASDFVQLPQTKSQFEKKVEEPVKFVKDEEYAKKTIVVLCQDVQSNLMALTYISMGTYALMENIDCQLQNIRRNNIFLCSYLSDIVLCFKKIAEACRDDGKNLKKQSSLLFYASTLIFFIANVCIEQRDCNHLVVREAIRQFDDSQLLNFLTRYIEHWRWSSRLSMRIRNMINLLFKILVLQFGEKPVWKRTKASIYDLHNLKYPSDSDKAQTLTISPLHYQAFREDVISRFPDYPTPLSGLPEEVDNSNSLSQFLEIPRPKARNPINLTLACPQQHIATPAPSPPSSPNITQMCDTIRHRKSFQTNMAFPCLYPSDDECGEDELTRRMPLDKEERESEVVVPYSVQEATNILSKSVHIKLSVQQLWYERDLFISTERGWKTNHNEDRYNYAVIPNVKKDEALEIMKRVDSYYNQCFSAFNSLVFVLLQTMESSLSNVDYRTADIPADSNIDSLIPRLEMARARELALKSSVGILCLMLKWFKLNHILKYEHLCVLLHDSRYIHTCCTLLGKYSESYSDKVLNRMLPTPISIWKECSNYNTDYQQSFLDGEPSNSKDCNVVMLTSLGYMLRVLRKIVGDKTERLKELPLNIGLLFKRYYRIFNLDIYHPILKIIKELTPFKNKRWKSEHMELISGVFLYEKLELLDNWVTGKDVTGELGDACGQEIAMRALLQFYNFLHYEKSMEDLGYSERSNSSLSLLNKESEYLGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XEN0</accession>
    <name>A0A8J2XEN0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">BN860_00650g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="HG316469">
        <property type="protein sequence ID" value="CDF91946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XEN0"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007234">
        <property type="term" value="P:osmosensory signaling via phosphorelay pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000004">
        <property type="entry name" value="Ethylene receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000289">
        <property type="entry name" value="Osmosensing histidine protein kinase SLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047:SF72">
        <property type="entry name" value="OSMOSENSING HISTIDINE PROTEIN KINASE SLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="23"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="19">
        <location>
            <begin position="468"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="17">
        <location>
            <begin position="533"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="18">
        <location>
            <begin position="974"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="376"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="692"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1101"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="386"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="413"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="432"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="744"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1105"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="14">
        <location>
            <position position="1029"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CDF91946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1131" mass="124868" checksum="06B16B5FA9D580E8" modified="2022-05-25" version="1">MWIKRLLFGKLEPPIRLGIRAQLTALVSIVALVSLIILAVSTGVYFTDNYKSLRSERLYMAAQLKSTQIDQNLNYLYYQCYWLSSRDTLQGSLANYVAGNKTYANWIDAGSVIQKFLTSSGLFSVATIYDSSFTPVLTATNNGTGNSISRSVLNKLMPLSGNSPLPSSLQTEGILTNPVLNGTSYIMSMSLPIFANPSIILTDSHVFGYITVVMSADSLKNVYDDTTALEKSNVAVLSAVYDANEKLTSYNFVFAPYGVSTDIISDTFPIKNNTFLNGALRQGKGGAIEKTKFLYNKKVAVGYSPCSVTFVNWVAVVLQSESVFMEPSTKLTKIISGTVVGIGVFVCLVTFPLAHWAVKPIVRLQKATELITEGRGLRSATPGTGSSAVSRSSSFFHRTSNSSVHSHGTDSGNQEKDHDLRSDHAPHGMFPTNGSINHSVAISTGSSGNEARSEKSRGLTTSANLIEARVPVYRRLFSDELSELTDTFNTMTDALDQHYALLEDRVRARTRQLEAAKIDAEAANEAKTVFIANISHELRTPLNGILGMTAISMEERDVDKIQNSLKLIFRSGELLLHILTELLTFSKNVLQRTKLEKRNFSITDVALQIKSIFGKVAKDQRVKLSITLAPNSIRTMVLYGDSNRIIQIVMNLVSNALKFTPVDGKVNVRMKLLGAYDAERSEQENYKSVYVVGGSEPKDGNQNGELDSSADPEDGSPTSREKRSDTEVTSEINETDEKQVSEEIEKEDEDSDEADDLSSFSTSTSSYNDTVFHSQFKKVPKVCDDSSDELGVEVKPQKTWVISIVVEDTGPGIDSSLHESVFEPFVQGDQALSRQYGGTGLGLSICRQLATMMHGTMKLESKLGVGSRFTFTVPLTQTREINFDDVPDAFEDEFNPDSKKNRKVKFKIAKSLRSRKSRSSLMTVGPKSSCSDKSLGQLKSIDSDRSVRLDRPFLQSTGTASSNINLSTAPNTNKVLVAEDNHVNQEVIKRMLNLEGINNIDLACDGQEAFDKVKKLAENEEHYNLIFMDVQMPKVDGLLSTKMIRHDLSYKYPIVALTAFADDSNIKECLDAGMNGFLAKPIKRAQLKKILEEFCPQNDSILDDEQQRGKQEQNEQDHEDQLQNEQGVDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2SWX0</accession>
    <name>A0A8J2SWX0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BN860_04698g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_04698g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2SWX0"/>
    <dbReference type="OrthoDB" id="2241241at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF50">
        <property type="entry name" value="HEXOSE TRANSPORTER HXT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="411"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="447"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="52"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF87363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="516" mass="57358" checksum="523DECA82AB4595C" modified="2022-05-25" version="1">MAEEQAAPMVKAEFFESESNLLENIPSHMPIKYIESVEDKEEVPSLLKPVMLCVAIAFGGFIIGWDVGVIGGISNMESFQNNFGPLVDLATGRHYFPDILIGLVISIFSIGGALGGLTMAKTGDWRGRKFGLYLSMFVYCIGLGIQLVHDVSWWQFFAGRMLTGLAVGSTAVLVPMFLSESAPLTIRGAMTTLYQLMITFGIMMGNILNYAVRSKLSNPLDNISWQLPIYMGFIWAAVIVVGLLFTPESANFLVTKKHSIEEAKISFAIMNGISEDNDASIDFIEQTLKEQHQTERHACHLNLFEFITGKPKYAKRLLVGILVMTFQQLSGINYFFYYGTSLFEKANFKDPYLPSIFLSTVNFVATFGGVYLVESLGRKYCLLLGSLGMFSCMLIYSTVGTFALEYRSSGIVMIAFTCIYIMFFATTLGPVSFVVVSELFPMKTKAISMAICSSFNWMFGFMISLLTPVITTRIGFAYGYFFAGCLILSAIFVWAMVPETKGKSESDINTLYETEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T0U8</accession>
    <name>A0A8J2T0U8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Potassium transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_05622g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TrkH potassium transport family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="9999863at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140107">
        <property type="term" value="F:high-affinity potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004773">
        <property type="entry name" value="K/Na_transp_Trk1/HKT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015958">
        <property type="entry name" value="Trk1_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051143">
        <property type="entry name" value="TrkH_K-transport"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00934">
        <property type="entry name" value="2a38euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064:SF30">
        <property type="entry name" value="HIGH-AFFINITY POTASSIUM TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064">
        <property type="entry name" value="POTASSIUM TRANSPORT PROTEIN DDB_G0292412-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002450">
        <property type="entry name" value="K+_transpter_TRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="4 10" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="106"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="737"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="778"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="810"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="872"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="937"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1027"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1055"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="199"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="291"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="624"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="970"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1182"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="323"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="358"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="523"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="536"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="981"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1182"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1228"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1248"/>
            <end position="1270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR002450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF87405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1294" mass="146467" checksum="2265BADEE9DDC30A" modified="2022-05-25" version="1">MISRTISRNPTLATFSQRYRDSFGKKLRDAIARFGHYVKPMRKYVFRNFLIVHYTYIITMTIVASIILYPIRNEKYIDILFLAAGTTTQGGLNTVNINSLKLYQQIVIYVTTFLTTPIIIHSTLAFVRLYWFERHFDGIRDSSKRNYKMRRTKTILGRDLTARTMSRNWQRSGTRQFTSASRRNNDDFQEKLFSGKMLHRDEQDSLPDSQNDRGRASKSSQGLGPDDIQRSASTKLQDDQNLHSGRGLSFKEAKPIRDNHHHQPYERFARRRSREISPEDMYRSIIMMQGHHQPNADDEGPALVIGAPGERSSGKSGVTSTKSEPEENKVDSPENTNERRPTHWANKNVSDGGESLTHDVNNQKGNPINGSFDINSNHSELMSDAEQQSNPFAASSVDSYSTFSGNSGVSSGSLTSDDRRTIGAADTSSSVRPLARFETPRQGINGPSIHFDISAPPSKRPDRNRVRKNRSNGSRSGFSMPKSKTLGQKLKRRLSFVDKPRMRSRNSFPDRSFEDPGRNNMENMDDYFADDESDKEDDHHSRVERQTHFPRSNTFDQRFSHSQDYLDVLGKSKSYDLGQTQDWNKLSSSPEFQKMIYKDWKRRHKGRKSLLKHRRRMETLNSRTGFKKFYSDPESDYGDSNSQYSPKGHAGSPENNEGEEHFGDTSMESRNSLDEEAGYFGPNDLGFVDSGPGGLSRSYSANYLSWEPTIGRNSTFVGMTKEQKEELGGVEYRAIKLLCRILVAYYGGFWIIAFLFLVPWMVTRHEYKKIIETDGVSPAWWGFFTGMSAFADLGLTLTPDSMQSFQKTSYPTIIMMWFIVIGNTGFPVLLRFVIWLMFQVAPDLSLTRESLGFLLDHPRRCFTMLFPSAATWWLVLTLVGMNAIDLVLFIILDFGSSTVHYLTHGYRVLVGLFQAISTRTAGFQIVDIGDLHPAVQVSYMLMMYVSVMPLAISIRRTNVYEEQSLGIYADPNEEEEEEAQQMVSSDEGNESESSTSTQAGNKKKKKKKRSNTSFIGAHLMRQLSYDLWFMFLGLFIICIVEGGKIRDNARPDFTIFAILFEIVSAYGTVGLSLGYPNSETSFSAQFNTLSKLVIIALLIRGRHRGLPYKLDRAIILPSNRLDNIDKQQEVTLRNRLARGTKPGDPVTAYFKSKTAAIKRGLLKIGKTAAEPEEHLLQEFNDHHTSGEDSENQETHEEHRAHGADEAHGANEAHEVHEIQEAQDSKGDSSPQESSSQPQEEAKAYVAQENASSSGTTASKRQSSDPYSTVPDQGGKSAHSVPSLRTYRLRRTNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T1C6</accession>
    <name>A0A8J2T1C6_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Superoxide dismutase</fullName>
            <ecNumber evidence="3 10">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_09142g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="3 10"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T1C6"/>
    <dbReference type="OrthoDB" id="239262at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.990:FF:000001">
        <property type="entry name" value="Superoxide dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.55.40.20:FF:000002">
        <property type="entry name" value="Superoxide dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050265">
        <property type="entry name" value="Fe/Mn_Superoxide_Dismutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11404">
        <property type="entry name" value="SUPEROXIDE DISMUTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11404:SF6">
        <property type="entry name" value="SUPEROXIDE DISMUTASE [MN], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase N-terminal" evidence="11">
        <location>
            <begin position="24"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="12">
        <location>
            <begin position="120"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000349-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF87558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="226" mass="25295" checksum="F0B5C13A79AC4D0B" modified="2022-05-25" version="1">MFARTASASLRQTPQFISRRTKVSLPKLDWDFAALEPYVSGKINELHYTKHHQTYVNGFNTAVDQLSELTTCLDKDPSPAVARKIISVQQNLKFHGGGYTNHCLFWKNLAPTSQGGGEPPTGALGDQIKEQFGGLEKLQKWTNATLAGVQGSGWAFIVKNLDNGGKIEVVQTYNQDTVTGNLIPLVAIDAWEHAYYLQYQNRKAEYFNAIWNVINWKEASKRFDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T1G3</accession>
    <name>A0A8J2T1G3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">BN860_09538g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_09538g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <location evidence="8">Coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T1G3"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09251">
        <property type="entry name" value="AP-2_Mu2_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14836">
        <property type="entry name" value="AP2_Mu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043532">
        <property type="entry name" value="AP2_Mu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043512">
        <property type="entry name" value="Mu2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0168">Coated pit</keyword>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="11">
        <location>
            <begin position="201"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="135"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF87573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="474" mass="53174" checksum="102D842E2A9CE376" modified="2022-05-25" version="1">MINAVFIYSSRGELIVSKLFNNSLKRSISDIFRIQVINNLDVRSPILTLGSTTFHHIRSNGSDSLWLVTVSRTNANSGAIWEFLYKLNAILDAYALTKEEKLKEDFMLCYEILDVVLGAGGIPIDTELGSVTSKLSVKPPKNSANSSESKVTGSFPGPSLSAPNLSMPKFLTRNNRSMSQDLGSNYPSSFPWRPNGIKYKKNEVFLYVNEKISILVSRDGSILKAYVDGTVDMTTQLSGTPICQFGLNESLSVEFGDSLWQETEHFTNKKAIPRAAAGSVMLEDCKFHQCVSLDKFNKDRVIKFVPPDGNMELMKYCVRDNLNLPFKITPIVTSCGTTVEYRVTLKSLFPNKLSAKDVALHIPVPPGTLDCKINTSNGKCRFVPEENAMIWRFNKYGGLTENTLSAVTVPTSDTTQLTLQQWPRPPMSLDFEIVMFSNSGLVVRYFRVLDKDEKYRVVKWIKYISKSGSYEVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T1V3</accession>
    <name>A0A8J2T1V3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">BN860_09582g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_09582g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T1V3"/>
    <dbReference type="OrthoDB" id="4068815at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014839">
        <property type="entry name" value="Crt10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08728">
        <property type="entry name" value="CRT10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF87575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="885" mass="100038" checksum="CBF0EA1827A1EB94" modified="2022-05-25" version="1">MSSDLDEAFAIWLASRRIVTRQHKPYLCFDSRILTAKGSAVLGNAFKIKHYLYDQQIHQGEFSTKATEIIEQHLQELERYTVQSLDCSDFVNKIKDRCDEVEPIWSSVSYVNPADYLVPPTQQLLVDQTLPKTDAVFFTPYAHHGLGFKNNLACVYGKWILMADKDVIEIHDLNCLLLEKTQWKEIKLRISLKSCKNHAKFQSDKELNKENIDLLFREDNFQVNFMKVTKFFGSDVLICCLDGGVVVLYEMDTLLNSVKDVTKSKILQKKPFLSAIPILILKTPDSCWSVDAIDKGSVTYLAAGHNGPGVTIFAFLEGHMSLQPIDTYQISSFHNVPNLNFVARSDDKNGSVVLAFCSIYGNVTTIKFKLNPIDGKIRAQALDSQFFAAFCWTVTPLSKGDFLKVPEFELLNLNYQTSFKSSILYSATQDSLIFGCHPPSVYCSGEIGIGGITTQIPVPVAPLEWGCQNGITNSLLGLRFTSFDKAGIITKSHLKPEGASSVISGYGPLFQLTRSSEPNLAERIYMDVPADMYRHYYMFERASLQTEHLNCEPCKKYENFHFNRAQSHHTEVSNCPNYKVWSGPLDEWIVEKPYHSHLLNEAYNLKCRMPTKVVVYSPAKSQAVSISDICGFTLAESFTDLADNCETWNVSNAENSSIPSQEPSPVDGLLAAGHIEEQPKWALHNHVRKVRHLLNLVEPESTSSPSGYRLSELNDDFFFVTTAHHIYLVKAHPLIVTSFTRDRIFPVSRITLCSCHEVLMALDRINIVCHIKEFHCMVVASQVGLLSLLRLTEYNGIYSFRQEYILGWNSQNAEDPDAQCILCYTNPGTSCRYCGIDDVILPFYHIVGLDYSYVPKDTLNCIGPYAILYVLSRNFLHRIKIAPSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2I3</accession>
    <name>A0A8J2T2I3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phosphoribosylformylglycinamidine synthase</fullName>
            <ecNumber evidence="4">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Formylglycinamide ribonucleotide amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Formylglycinamide ribotide amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">BN860_15984g</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the FGAMS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="4"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6666987at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01740">
        <property type="entry name" value="GATase1_FGAR_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02203">
        <property type="entry name" value="PurL_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02204">
        <property type="entry name" value="PurL_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.750:FF:000002">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000002">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000005">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000008">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000002">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.750">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase, linker domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00419">
        <property type="entry name" value="PurL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040707">
        <property type="entry name" value="FGAR-AT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055181">
        <property type="entry name" value="FGAR-AT_PurM_N-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010073">
        <property type="entry name" value="PurL_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01735">
        <property type="entry name" value="FGAM_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003672">
        <property type="entry name" value="PRK05297.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18076">
        <property type="entry name" value="FGAR-AT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22689">
        <property type="entry name" value="FGAR-AT_PurM_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13507">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01211">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109736">
        <property type="entry name" value="FGAM synthase PurL, linker domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="PurS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase N-terminal" evidence="21">
        <location>
            <begin position="41"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase linker" evidence="20">
        <location>
            <begin position="207"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="19">
        <location>
            <begin position="472"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="FGAR-AT PurM N-terminal-like" evidence="22">
        <location>
            <begin position="689"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="19">
        <location>
            <begin position="880"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="339"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00057317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00071729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF18072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF18076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF22689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CDF87855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1353" mass="147319" checksum="1AB64E3F37B8421E" modified="2022-05-25" version="1">MSRQILVGPRALSQFRVDNLVESVSQHSNSTSIIKLVRSCYVHYVDATVPELSAEDEKLLKVLLTYDTPLDVTTDPLSKLLADAVIKNVDDPILDDDTYLVRIVPRSGTISPWSSKATNILTVCGLEGKVNRVERGLALAIKVIPGFPLLQNLNDESLKTIFDRMTQELHLTSAPSTDDLFTHHDPKRLLHIPLVSEANEVPPQTTLAKANKEFGLALDQGEMEYLINAFVETLHRDPTDVELFMFAQVNSEHCRHKIFNADWTIDGLKQKFSLFQMIRNTYNKAPDFTISAYSDNAAVLDSGNVAYYYTPDPNTKQWVATEEKVPMLIKVETHNHPTAVSPFPGAATGSGGEIRDEGATGRGSKSKCGLSGFSVSDLLIPGYEQPWELDVGKPNHIASSLDIMVEAPLGSAAFNNEFGRPCINGYFRTLTTKVKNSKGKEEIRGFHKPIMIAGGFGTVRPQFALKNKPITPGSHLIVLGGQSMLIGLGGGAASSVSSGEGSAQLDFASVQRGNPEMERRCQQVIDACVALGAKSPIQSIHDVGAGGLSNALPELVHDNDLGAKFDLRKVLSLEPGMSPMEIWCNESQERYVLGVSSRDLPVFQQICERERAPYAVVGQATAEQRLLVEDSLLKDTPIDLEMPILFGKAPKLSQQTTTQNLKLAAPDLSVIPSVREALERVLRLPAVGSKSFLITIGDRTVTGLIDRDQYVGPWQVPVADVGVTATALGSKIVTTGEALAMGERPTNALISASASAKLCVAESLLNLFASDVKSLKHVKLSANWMSPTSHPGEGAKLYEAVQALGLDLCPALGVAIPVGKDSMSMKMKWGDKEVTAPMSLDITAFAPVNDTGKTWTPVLKKNVPNTVLVLVDLAASVATKSLGGSSLLQVYNQVGNTSPTVHDNATLKGFLEALLELHTMDIVQAYHDRSDGGLIVTLLEMAFAAHCGLTVSIDDTQETSPLVPLFNEELGAVFQIADSKLLDFKLVLSRHGISARHVSIVARPEFDTQAIKIQDAQGELLYESTRGQVQQIWASTSFHMQSIRDNPKTAEEEFTAIKDDKDPGLHYTLTFNPTDDLGLGAKLHQAGPRVAILREQGVNGQMEMAWCFQQAGFTAVDVTMTDLLEGRANLDDFVGLAACGGFSYGDVLGAGAGWAKSVLYNEVARSQFVRFFQEREDTFALGVCNGCQFLSRLKSIIPGCEDWPTFATNLSEQYEARVCMVQVFSPEDQEGSSLFLRGMGGSHLPIAVAHGEGRAIFDSKEQLQRFEKKSLCSIRYVDNRGNVTEKYPFNPNGSVGGIAGIKSPNGRVLAMMPHPERVSRLAANSWYPEEKFDEWQGYGPWIRLFKSVRASMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2L0</accession>
    <name>A0A8J2T2L0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_16424g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_16424g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2L0"/>
    <dbReference type="OrthoDB" id="6508832at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23807">
        <property type="entry name" value="UEV_UBE2V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000026">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="5"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="138" mass="15599" checksum="95B9DCDC7F27B738" modified="2022-05-25" version="1">MSKVPRNFRLLEELEKGEKGFGPESCSYGLADSDDISMTKWNGTILGPPHSNHENRIYSLSIECGPNYPDKPPKIKFISKVNLPCVDPKTGQVSPTTFHTIRDWKRAYTMETILLDLRKEMAMPANKKLAQPKEGTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2N3</accession>
    <name>A0A8J2T2N3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BN860_09076g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_09076g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2N3"/>
    <dbReference type="OrthoDB" id="4082933at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000160">
        <property type="entry name" value="Probable beta-glucosidase btgE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF24">
        <property type="entry name" value="FAMILY 17 GLUCOSIDASE SCW11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00587">
        <property type="entry name" value="GLYCOSYL_HYDROL_F17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035185421" evidence="9">
        <location>
            <begin position="21"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="70"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF87555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="461" mass="48468" checksum="100F443BFE68AE9D" modified="2022-05-25" version="1">MIDTIQLIAGLALLHSFAAALPVKREIITRMHTAPTHTNYDVYSTTTRIHVAPTVEFIIDGQNTYTTTLIPSNVDPDAEPATSTLYGTAQQSSSQAPAAPSSSEAAISSPWSSSAAPTEVATSIQWSASAITTQAPASSSQQWSSSTWGSSSALPTTTSYASSSSAYASSSSWTSSTSSYSSSSSQASGSSGSGGLSSVPTAITYTPYNDDSSCKSSDQVSSDLQKIQSKGISKIRVYGTDCNSLDTILSAASSLGIKVNQGLWIDDQGVDSIDDGVEALIQYGQSNGWDVFDFITVGNEAINSGYCSVSDLISKIKSVKSQLQNAGFSGSVITSEPPVSFENNPELCTESGIDFVGVNPHSYFDSGISADQAGQFVKGQVQLAQNACPNMKIVVTETGYPSEGDQNGDNIPSASNQLIAVNSILQEMDNDVTILSFFNDKWKNPGPYGIEQWFGVEDILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T322</accession>
    <name>A0A8J2T322_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_09406g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_09406g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T322"/>
    <dbReference type="OrthoDB" id="4066363at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="273" mass="30531" checksum="338AE33279FFB3E8" modified="2022-05-25" version="1">MQRRRFRLLNERESIRPLHARSTNVKPSAGRDVRVKQKQSLKRKSDESLMPTKRRRSERHPEPSQISNTVFDNDHVQLRLLDSDDLLPGTVSPSERLTNIEEEEDSLQLEDFDQPPESTSTPLSAPRAMEVSQDSFRPPLCYNANMDDSPLQDKRYELDTYPCANYLPMGFLPQYPSHPGSHLGYIQPLGPIMSSAAGPPIRPPIGQVVNMPNMPYPIRGPVGAPVFTDQYFGAYGYGAMFPPITSQNSGSSSYKSSGGDATYQGSNESSEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3G9</accession>
    <name>A0A8J2T3G9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BN860_01266g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_01266g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1562405at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019441">
        <property type="entry name" value="FMP27/BLTP2/Hobbit_GFWDK_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019415">
        <property type="entry name" value="FMP27_SW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019449">
        <property type="entry name" value="FMP27_WPPW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045167">
        <property type="entry name" value="Hobbit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678">
        <property type="entry name" value="ANTIGEN MLAA-22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678:SF15">
        <property type="entry name" value="PROTEIN FMP27, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10344">
        <property type="entry name" value="Hobbit"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01214">
        <property type="entry name" value="Fmp27_GFWDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01215">
        <property type="entry name" value="Fmp27_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01216">
        <property type="entry name" value="Fmp27_WPPW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27 SW motif-containing RBG unit" evidence="4">
        <location>
            <begin position="1142"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27/BLTP2/Hobbit GFWDK motif-containing RBG unit" evidence="3">
        <location>
            <begin position="1263"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27 WPPW motif-containing RBG unit" evidence="5">
        <location>
            <begin position="1682"/>
            <end position="2133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2559"/>
            <end position="2594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF87210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2620" mass="299907" checksum="5643332952580B0F" modified="2022-05-25" version="1">MDSDLARYCFIYVVLPLLLCNLLLFKFAGATFWFINPFRIEVVNLRLQGKCAVRSLRLSLRKKKIIVEGLTIGAIDADDTVASEGSDEGKSQIRYKSPQYRLEKYLPLLLRICNGLRIEISDLSVEHVHKYSIKEIRAAIKYKTSTGLLNVNVIVSSAHWAGESVCQDFSLKLSTKLAGQTLNKHDLFSEIKADLKIFDVLIPLDAVRALDRSPASSSSTCKNCRPSHSYVRPEEPEFNASIQEGLRDLYEKIDTLRLTMAPISQFNVVIDRLAIRGVSLAQIPELQELNEALTYNIYATNINFDTIRYESTMPGFKLLFKDDDTPIKLGLTISNFAICLDMKRKCNVPSSNLIKVLEIPNISLYGSTNLFTQYFGDEDDSSLKNAILSLKCNVLTPTFDINVNHLSFYKCFKKNIRVFANSLKDNSNDVSNASYQWRKHLLIAYFENFLPLIDIKFTLEDTRVVISDEDDILIHKLTALMLRYNTERSLSIVNGKGEQIYDGRIGAEVLGLRLQHLNKNLDYSRGIASLESLVLRSALDIVPNLLLSSTLELDAIEVDLSELPTMIALNNIFRKLDIQIHDVEQNYFKPFYEKFAERLQLAEQACCTIRAEAKSHEILPSSFVFKELPAIFDYIKVTMRDLKMYLGCRSVFMPPETFSNIDAQSPDDLVNGKLRKLFHKTDKVELAFFGTKTQWHNKEDGARIRMVKSAQLSDYRYYDGDDLDNFSSSAATEVEHLWNVNILVDNISLTVIAETPESVKELSKKKMIEISAFTMAIYPDIDQLDSQGGSKLMLKCDTKNVYSVTSLMLAFFVISGIHTLNQIFGQDIMLQNQESMAKTHLMALSQAKTKSFLSHVEWKELKKYLNIEVFIERFNQVLDLPNGLKTRLEAFSTFISLTNMSEVSIKGEYFRLCVESPTVKRYWVRLVSITHFKVLTDLTRLKEQTKEKAGNFDNLLPSVTLENESWIFNIPHQFEMYRLFDNISTVVKAFKQMIYSFKSCKNSNVIFPKVMSAPYLPKVKLKSGRLNFTIDDDPFEKQMSMIFQIGLQEQKIRLAKLQEFDKHIERELMFTGKPHLKQNNTISSHAKKCDICNKINAIKNKLIFKKKSPHSKSSFSNESSFTPVDDYGGEYREHGILERASKLYEKLEENFSTSWINRIEQFRYKERESFFKSFSYLWGNVDYSKLPPDVNYRVLPFSTSPFLSIITIEGIDIDIFQPSFGVEATANFVYNLGKKVPTDTQYSFIFPMYLDAKFEELRWHLRDYPLPFIYIPRLEPSQVKDKSAIHIHGDFVVGEDMIQSERELRTLFIPLIPSITVENTDCYYSLLIPRTITAVKVFADLNLDINSKQTTQVTWGGGYQPAIQQAMQCFDNFSKPPLDPSAKTGFWDKLRYLFHAKVLISWKNDGRFEVGLKGSRSPHKIGSDASGFIVGFSKNISLSVNEEGDPKKFVSCSAEKIYFSIPNYFAKPLLVWSRPSTETVFVPINDETTLQECASYYYLSDLGTGVKDASKLRTMRSHYIEKTGIKLAGGVTLNVGTVFERRIPGTKQRTFDSTPHYNVRLSNPIYVEDINKHDSFSGFRSDFIHLSFTLLSASTSAYNALQLSPAGLVTFLKWWRSFAGNFPVRSGPLFGLQSMSPKFGEHLFTISYHADVSPLFLSYIAHSVDTSRLSKKSQSDNPEFAGVKAKATKFVMDLHQRKEVLITYQEELNRTKQIMKLNFLAGEVWTKKFDIRTVEGSFKPMTSQHDKKDAFFEVYDNDMAWYDVSDFNEAYSVDLENCVPKVRIQPLLYTPHFVYRKHASYGDKYQVDPKDHQPIPPFENGISHDCSLGKAKLLQLGILEERVQLLKDLKAKSINGEDSNLHHGNQQELLAKLSLGIEKAQHLLEDFRYMHDHENGPAGSHNFYYELPNLMRESMAQVYENRYCINYMLLKWNEDTRDAMYKFLYFLGLADQFSALSASKSLQIIEDVVRQKTDYDQMRSRQEDDRVPHEKRTVEIDLSNLKDNELNQSLSKILEKHLTELNISVKNVSRSNHYVEFLSPQIQLITNEEPDACIMVTSPGVKLEVVSFEGDTTNDEYRRDTIMKRYGVHVVNANVFYFRREDYMDYLDLYFNVAAYGHVEGTSWPPWVALELCFDSDYIPAEANIVKNLSAAICYDKVSELSSLYGLVKDKLQDKLGGFFPRVAVSSDSKTYMYIHKLVKNLFFYVEPENAELRKQIDKLTIGFDLGDVSQMRDVIEGLHSSLRILTTVENGFLFKRHILDDADKVELHNVQSERVHHLLRLYIMMKVFTSNSKWTDSAHSSLLWDFNVGKVILHLLVEKTKPFLDIGIERLHFERLESAGVFNNNKLTASKLQVNDLRDGVTFQNIVGPISPKHLTKDEMTDPSPLVFIMWEMDEPIGGIKVAKKAHTRITGLSLNLEDETVMKIVRWFMPDEIKPMLESDDSDDSESFDLDNAIADAEENGDAEILLDKHADLNEMVQRSSEFVIIESLHLNTFKVCVSFRGKGALRLANVTEFVYNFPTLKLQNQTLRVMDLLILLKKVLIKDLVRHTGRFLGTKVRRHRPDDDSSITTTYNGDAAPAESLRRLPSSSNSINSGSALISRSIKRLPSFSSNSKDVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3P0</accession>
    <name>A0A8J2T3P0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Telomerase reverse transcriptase</fullName>
            <ecNumber evidence="2 13">2.7.7.49</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Telomerase catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_07932g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.49"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Chromosome</location>
            <location evidence="13">Telomere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the reverse transcriptase family. Telomerase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.49" evidence="2 13"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3P0"/>
    <dbReference type="OrthoDB" id="289721at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000333">
        <property type="term" value="C:telomerase catalytic core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003720">
        <property type="term" value="F:telomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01648">
        <property type="entry name" value="TERT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021891">
        <property type="entry name" value="Telomerase_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003545">
        <property type="entry name" value="Telomerase_RT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12066">
        <property type="entry name" value="TELOMERASE REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12066:SF0">
        <property type="entry name" value="TELOMERASE REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12009">
        <property type="entry name" value="Telomerase_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01365">
        <property type="entry name" value="TELOMERASERT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00975">
        <property type="entry name" value="Telomerase_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="9 13" id="KW-0779">Telomere</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="14">
        <location>
            <begin position="407"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF87506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="862" mass="99236" checksum="E6F1D3768E123AF3" modified="2022-05-25" version="1">MKSLQAFIEENLHIDLVSTDYLRLTASGALNGLDELLTTCFVIHNEHRIELPDIYPGARYKDIVDTCVIHLLKKKLYNNILTFGYRIAVSSEVNTSLHCVSTNSNVSRIKGKQWQLLCRIIGIENFVDLLMNYTVLQYIDKFFTQIVGNRANERHVPPSWCSDNRDASCGSHDVPITIQKLLYRNASPFNEEQVLPAGDDVEAIINEIFGPSSYSKLCFRKSKVIPIIKKMIQNHAGLKYLQILNSVCPFDSDFVEGKHLDASTKVTLVNKFVIIILQKLIPSEMFGSKRNKSKIFYYLSQLLRLPLKGTIPFSELKNSQRIEDFRWIRLVANCNESSLQNNQRLVACFISWIFKLIIPRIVTTFFYATEVSSSVLVLFFRHDAWSKISAPFMKDYVDKYLIENEVCRNHNSYLLSKYNHSRLRLVPKKAKGEFRVLAIPFKGADDEEYSIFQHNFKTMILPVQCILEYLRKKRKTHCRKLYSPTQIAKHLMRFKKVLLTKYGKIPDLFSMKFDIESCYDSISRKKAMQVIGVVLGNETGFFVRSQSFYQPETGILKIQNVVNGCRQPAEDEIYIDNVRTVYLSKEDVCDVLKMELFDTALCLAGKCYLRRDGLFQGSRLSALVVDLVYDDLMERYEIFRGQYPNESLSLRLADDFLVISTDRNQILELKKLSSDGFKEYGAKIKAEKIKISSSICLPHKPFQFCALNISLASLQAWKNSESFNIPYICFASTSKTYDQLIKLLEMRLSYGTTDSSLNTPFTILFQIHEVCVNVSQTFVGAFRKKIILLQPFENFILRLLVSTIKACQNCGCDPFFQSQLRQLVFQTFLETLLPIPSKFVEVIRYLKFELNKCTYISSVAPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3Q2</accession>
    <name>A0A8J2T3Q2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_14906g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_14906g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3Q2"/>
    <dbReference type="OrthoDB" id="70250at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007720">
        <property type="entry name" value="PigQ/GPI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21329:SF3">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21329">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT Q-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05024">
        <property type="entry name" value="Gpi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="385"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="417"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="454"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="484"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="618" mass="72173" checksum="8177F0E3C9D34889" modified="2022-05-25" version="1">MSRHYIFWPQNLNDRKIIGCNESLAAVAIALRGTDYVVLDALPMSLVEKERLESPYTIVGIKDSGSNRWKFSDEMMCIIEFRVPKIELLQFFSLGPISLTLPEKEYETNHDCKETKSLRQLWGHPRYQAHDKRLRQSLKLLNLFNTHLSALHDKYPYHFTHTNKLGQKIFVVVKSWTFMRTVRNAFAYVSFYLTISACRIAACIYLILNWSYLPLVNISVTAQQIDLRCQQVCYFPVQYLRINMNVSLRKALPRFRTYSEASANLRKDLPSKYYPDYIRFYNTLWLMLNDVSFGLICGSLLLEKCDDIAPLLHKVITYCLYDFIKTVTLLLAQNPGGIKLNEELASFLSDLFLWTIEFSNAMFIRPLADTMNLRLFIRILANSNCIFGGTFALSLIVDYFSILSIHIYLFYRISSKIYYWQLNIMISLFYLFCGKKKNILRNRVDNNFFQLDQLLLGTLFFIILVFLTPTVLACYIAYTILRMAAITIEILLESFISLLNHFPLFALLLRLKDPRRIPGGISLRLKERTSDLPHFELKNKPLSIYLMFKPFVVLMDQKRDTFYSTKTLKKILLGKPIAIKRNKLYQILYSSLPNTPIDIKSLYDKLEHLSTAKEKKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3S2</accession>
    <name>A0A8J2T3S2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Mannosyl-oligosaccharide glucosidase</fullName>
            <ecNumber evidence="11 12">3.2.1.106</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Glucosidase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BN860_09054g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(4)-(alpha-D-Glc-(1-&gt;2)-alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + H2O = N(4)-(alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:55988"/>
            <dbReference type="Rhea" id="RHEA-COMP:12806"/>
            <dbReference type="Rhea" id="RHEA-COMP:14355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:59082"/>
            <dbReference type="ChEBI" id="CHEBI:132537"/>
            <dbReference type="EC" id="3.2.1.106"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="13">Glycan metabolism; N-glycan degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the glycosyl hydrolase 63 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.106" evidence="11 12"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3S2"/>
    <dbReference type="OrthoDB" id="410058at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004573">
        <property type="term" value="F:Glc3Man9GlcNAc2 oligosaccharide glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.110">
        <property type="entry name" value="Glycosyl hydrolase family 63, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031335">
        <property type="entry name" value="Glyco_hydro_63_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031631">
        <property type="entry name" value="Glyco_hydro_63N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038518">
        <property type="entry name" value="Glyco_hydro_63N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004888">
        <property type="entry name" value="Glycoside_hydrolase_63"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412:SF11">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03200">
        <property type="entry name" value="Glyco_hydro_63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16923">
        <property type="entry name" value="Glyco_hydro_63N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035250601" description="Mannosyl-oligosaccharide glucosidase" evidence="14">
        <location>
            <begin position="23"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 63 N-terminal" evidence="16">
        <location>
            <begin position="37"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 63 C-terminal" evidence="15">
        <location>
            <begin position="311"/>
            <end position="813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CDF87554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="815" mass="93536" checksum="23BCC828EE3904DD" modified="2022-05-25" version="1">MLVKFLLHITLWLNIVIGSSSGEPFNDASFEKYTKQSLLWAPYRSNCYFGLRPRNVDATPLIAGIMWFDNSRTDGITKFRHFVDQGDRMTKYGWEMYDPRLGGKEVIIDDENNLNLTIYFTKSHDGDNWAFRVHGEPLDFTRSTTASIILYLNQNGDPKESRLMKASSEGDSDFLQFDGLSKELGEYQISIWDNLGELFNDRDLRNMEVAPGCDASKTAHVSLTVPDGSVWKAKDIFQTLMADSVKAILEEKKADVHTSLIPSIFTIRNYYQFPAGNFHYVQKTFDNSDPKGFEFDVVYNKLESQERINTPQQVSSLIASTLDEVTARFNRHFHFKSQNNGHRQFAIETLSNLLGGIGYFHGNQLVDRTTELDEDTFKEVQLTHPVEEGPYSLFTSVPSRAAFPRGFYWDEGFHLLQIMDYDFDLAVEICSSWFELIEDSGWVAREVILGNEARSKVPDRFAVQNPNIANPPTLLLAFSEMLGRAIEQENQDSLNRFGENTEDSLTPQTNQLELNSDLLINYAKKIYPKLLKHYNWFRESQRSSLDDYLDVLEEEGILNEIHLNETYRWRGRTVTHCLPSGMDDYPRVQPPDESELDVDALAWVGVMTRSMRQIAHILGLEEDEAFYQKIESNIVENLDTLHWSEDAGCYCDITVGGEWDDERTFACHHGYISLLPFALKLMPQGSPKLDRLISLMSDPKEMFSDYGLTSLSKSDLFFGKGENYWRGPIWMNINYLVLDALKYYYPDVVATSPKKSTEARSLYQALKKNLINTVFDNWEKSGYCYEQYSPENGKGQGIEHFTGWTALVVNIMGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3U4</accession>
    <name>A0A8J2T3U4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Mannan endo-1,6-alpha-mannosidase</fullName>
            <ecNumber evidence="4 14">3.2.1.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BN860_14158g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Required for normal synthesis of the cell wall.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>Random hydrolysis of (1-&gt;6)-alpha-D-mannosidic linkages in unbranched (1-&gt;6)-mannans.</text>
            <dbReference type="EC" id="3.2.1.101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyl hydrolase 76 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.101" evidence="4 14"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3U4"/>
    <dbReference type="OrthoDB" id="4187847at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008496">
        <property type="term" value="F:mannan endo-1,6-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007117">
        <property type="term" value="P:budding cell bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000006">
        <property type="entry name" value="Mannan endo-1,6-alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005198">
        <property type="entry name" value="Glyco_hydro_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014480">
        <property type="entry name" value="Mannan-1_6-alpha_mannosidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145:SF21">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DFG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03663">
        <property type="entry name" value="Glyco_hydro_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016302">
        <property type="entry name" value="Man_a_manosd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035210230" description="Mannan endo-1,6-alpha-mannosidase" evidence="16">
        <location>
            <begin position="20"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="431"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR016302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CDF88605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="453" mass="49703" checksum="00B2F4049D06164A" modified="2022-05-25" version="1">MLLMNIIAWLLHAIIPVWALDLEVESKDSVCAATALIQQGMLDYYAGDKYGGAVGMFQSPYYWWQAGEAFGGMLDNWYFCQNNTFENLIRKAMLAQTGTKYDYMPDNQTMVEGNDDQGVWGLTVMGAVERNFTDPGDGKPGWLAMAQAIFNEVWSRWDDEHCGGGVRWQIFTWNSGYNYKNTISNACLFQLAARLGRYTNNQTYLHIAEEVFDWLHDVGYVVLSDKGNVYDGAEIDSNCTEVTRLEWTYNHGIVLGGLAYMYNATNGSSTWEERVSQILGGATDYFFENDIMYESGCQHNDSSTCNNDQRSFRSIFSRMLGLTSVLVPSVSDQIDTLLTASAAAAAKSCSGGTDGHTCGLNWFKGAWDGQYGLGEQMSALEVIQNTLVHTRPAPFTASDGGSSVGDASAGLNSSTTNVLQNKLSINTGDRAGAAVVTAVVLMIVVGGSVWMLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3V2</accession>
    <name>A0A8J2T3V2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_17414g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_17414g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3V2"/>
    <dbReference type="OrthoDB" id="424794at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02557">
        <property type="entry name" value="PseudoU_synth_ScRIB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluA-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF42">
        <property type="entry name" value="TRNA PSEUDOURIDINE(31) SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="1">
        <location>
            <begin position="121"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="400" mass="45851" checksum="138C9B86F1C6446C" modified="2022-05-25" version="1">MGVQVYFSHGLRKLRPYYHTRGAFAKGRWFGKTLLDVLLNEFRSHSEQHYLEEIRKGRFGIIRQGESLTPEEVLLSPIRNNDIVQTLSHKHEPPVLQWSAEEDDVPSLKIAGLDVIHEDENLLVIDKPNGIPIHPTGQFYQNTITEILKNHGKAALPCYRLDKLTSGLLILAKNTSTAGKIQERIRDHDMIKLYIARVKGRFPHSTADAIERATPFENPSFITQEVSSVYTIEPKKGFPAGLSAVKDAATSFYPLKYFPGCDESVVICRPLTGRTHQIRIHLARLGHPIVNDPFYNVDNVEYKIRSHFIVNISDWTELNYSTEQLSKIFDTFIQESDQVQEERTNDQKYPVRCPECGSVSLSDPEPDKMKIWLHAWKYADKQGELKFCTRLPPWTDQLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3Z4</accession>
    <name>A0A8J2T3Z4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BN860_18822g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_18822g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3Z4"/>
    <dbReference type="OrthoDB" id="411372at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045041">
        <property type="term" value="P:protein import into mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052604">
        <property type="entry name" value="Mito_Tim_assembly_helper"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016694">
        <property type="entry name" value="UCP017292"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008913">
        <property type="entry name" value="Znf_CHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037274">
        <property type="entry name" value="Znf_CHY_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28082:SF1">
        <property type="entry name" value="HELPER OF TIM PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28082">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05495">
        <property type="entry name" value="zf-CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017292">
        <property type="entry name" value="UCP017292_Znf_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161219">
        <property type="entry name" value="CHY zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51266">
        <property type="entry name" value="ZF_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHY-type" evidence="5">
        <location>
            <begin position="11"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF87977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="109" mass="12492" checksum="4ED584F0811A0CAD" modified="2022-05-25" version="1">MQGGNLIRGKLIDDESRCVHWHSPLDVIALRFRCCDRYYACYSCHSESETHAVEKYSIRSSSAANVPLIICGVCKFQMTFQQYQGLQCPSCSAKFNPGCKLHYHLYFET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4A8</accession>
    <name>A0A8J2T4A8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BN860_17062g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_17062g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4A8"/>
    <dbReference type="OrthoDB" id="1058301at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034479">
        <property type="term" value="F:phosphatidylglycerol phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08570">
        <property type="entry name" value="GDPD_YPL206cp_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052271">
        <property type="entry name" value="GDPD-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42758">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42758:SF2">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GP-PDE" evidence="8">
        <location>
            <begin position="2"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF87902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="315" mass="36405" checksum="8D7058737A7D5CF2" modified="2022-05-25" version="1">MVLVAGHRAFKAQYIENTMVAFDRAYEAKADIIETDLQMTSDGVVVVNHDADTGRIWDQDLCISDHTFEEISKLRCKDDSTLRMPTLIDILKWMVDHPNVRLMLDIKFTNQKIVLPKTYAAMLGVKNDVQFWQKHIIWGLWQLDWFQYGVETGAIKDFEVFVITLSLDIAEQFIKYSLKLNNPHFKLSGISIHFVASWTQKFRYHLAPILQENDIKVFLWTVNKGIDFKYTGGLPIAGVVTDDPIVARQLSNEYSSIKKFVPPSWGSLNGLRFYSFLAAYHIICTLLFIPWAHYKVGPYSVAYLVLYLLRTIHFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4B3</accession>
    <name>A0A8J2T4B3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BN860_13410g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_13410g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4B3"/>
    <dbReference type="OrthoDB" id="20729at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051767">
        <property type="entry name" value="Nucleoporin_NUP42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46527:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46527">
        <property type="entry name" value="NUCLEOPORIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="215"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="271"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="423"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="483"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="291"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="425"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF87742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="598" mass="62066" checksum="1720DF90A6EDE97D" modified="2022-05-25" version="1">MSYTNSGRVPCKYFQQGRCTRGNSCKFAHVRSNGTTTTGEGTQNMSEADIYRSFVSPSSLNKIERTIANDIRDVENFQQKPLTSAYSYGVPCALNLISGRDYSPEESRFDFYKSQRQGTGQQYIAQINAREKDMQKCLSHVKSHPDWAARYLQKNTKDLVETGQRSMKNEFVNFPIDLGGGNTFGTSTFGTSTQTSSGPFGRPTSAGAFGTPAFGASSFGTPPGGPVNTGSSVKPPIVRENPFGSKKLNNMFGTSSNPFIANSKSTTGAFGQPAFGSTSRPGAEGSSFGKPAFGSSAPTTSASSAFGKPAFGSSAPPASGGSAFGKPAFGSSASPTNGGSAFGTPAFGSSPAVANVTSAFGKPAFASTNSNAFGGVAFGKQAFGSASTPFNNLQTSGNTTFGQPTFGSTSLSAMGAPPFGKAAFSSTSTQNDSTTSAFGQPSFKSSSPFNGNGNSQATATFGSSNVTAVQAGLPFGSGNSPFGSTTVNSSSPFTQIQSNAQSTPFEPSAFVSKAQLQNPSSAVPSSAGTTFRIQESFSKASTPLQTQVRQRKFIQGKPAAEDNLTAKELDQQTLRCFRSSHFSLGNVPDIPPPLELVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4C1</accession>
    <name>A0A8J2T4C1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_17766g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 21 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4C1"/>
    <dbReference type="OrthoDB" id="526653at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.10">
        <property type="entry name" value="Mediator complex, subunit Med21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021384">
        <property type="entry name" value="Mediator_Med21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13381:SF0">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13381">
        <property type="entry name" value="RNA POLYMERASE II HOLOENZYME COMPONENT SRB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11221">
        <property type="entry name" value="Med21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="87"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF87933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="144" mass="16619" checksum="F75C869C6F464424" modified="2022-05-25" version="1">MTDRLTQLQICLDQMMEQFCATLNYIDKNHDFEPTDEHEPKMSDRHATVAPPEEFSNTVDELSTDIILKTRQIIKLIDSLPGVDVSTEEQMHKIDTLQKELVKIEDKKIAAVRDKEKLQKEVNDVIDFFVSGIAESRQESVEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4E1</accession>
    <name>A0A8J2T4E1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BN860_14092g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_14092g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4E1"/>
    <dbReference type="OrthoDB" id="129353at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17745">
        <property type="entry name" value="BRCT_p53bp1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047249">
        <property type="entry name" value="BRCT_p53bp1-like_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013914">
        <property type="entry name" value="Rad9_Rad53-bd_dom_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047252">
        <property type="entry name" value="TP53BP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15321:SF3">
        <property type="entry name" value="TP53-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15321">
        <property type="entry name" value="TUMOR SUPPRESSOR P53-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08605">
        <property type="entry name" value="Rad9_Rad53_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="5">
        <location>
            <begin position="831"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="128"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="309"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="361"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="513"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="782"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="272"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="361"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="384"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="556"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="574"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="782"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF87770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1131" mass="129036" checksum="DB1E20FB8A8DE222" modified="2022-05-25" version="1">MVHEIKQADESALNYATDAAMQETPIANHFFSPKKAPKANSIFENKNTPTEEFKFNSQAHADQCCCSPLTAKGMEGKVFNNESLQVSDESFHHSPPILNRRTPDLDKIAEFFHRNRTPKQSKLLAQFESSELQDTPESQRRNLSANIPKINKKSSSTEFIRNPRRTDEGQCSEDTESDLLYKSNIRNSEKSLEFIKDAGSRKNLMSFNGRTQLEEKKTQRQIDHMEPVNKRDASKYEIHSNGVQNSKFSENSEKTMKISFINSDPRKVYEGQDNNGTDNEISRLNDTTDGDLPVQWVFRSHAHELYNNSNRSTQIIDPTETQEDNDTVNERETSQNGDTVARLADDTQTQLVASFNGQGESFLNETGDVDSSTQVVRSPEQLLPRNSQGTPSTSSNLFFEPITEVPETSSPPKPREVIDIASSANSSISEQQAPSNETEEHEKASQERVFLQISNRPEHFPSQEIELSECMDTKKEIAAASSEVELTQDLPEVEEQGTQEKIYNLPARSFDTSFENSQEITKKTSEKRRKNDRIAIEEEVDEGYRNHPRKKLRRSSPCNKAQNQMRTPDRSPFLINQSDTSPSPFQEDSNGLSGPPVEDDRKFPQAIRSQDDKFLSKEDIGFEDAVWCQYDLNYSFYPGRLLSYDESSDGCWVVFNTGKSLTKNDDIYYLDIRVGDTVNWKGKPHSVVALECRTHDLNIIRCIRGFDTVHLRRRKVSATLGKRSFVVPLSSISLDVNEWTKRPKVILEEGSHTRAKAFKFLQHPIRGRKNIVNTSPRKARVESEKTRVLTYKEDSEDESSESGTSSSMSQLLDLSSKIGPPHIKKKLLDAKELKIFERCIFVLSGLNQDKDELLRVIEDFGGTVLELGFLELFDYECLDSRSQQMGDAYLLNLLLKSDSKLDFGNFKFACLLSEKHLRSLKYLETLALGWPTVHWNFIEECLQKAKLCTENLHQYLLPSGESHRLAADAHHKLGVIKSSNIFDFYETLLRGGSLRDQVSIKKSVLFGYTILLYGHSELDQFIRFVLACFGVKRVFQFAGKIENAACQNLDALTKRLNFLLAKDKDCRIIFYINGDSAVSDTILEENKRKFWKYFANWENLPTDFHVESKEWLIQTVINEHAGFVEKNIFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4H5</accession>
    <name>A0A8J2T4H5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_15786g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_15786g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4H5"/>
    <dbReference type="OrthoDB" id="5599713at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037521">
        <property type="entry name" value="FLCN/SMCR8_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021713">
        <property type="entry name" value="Folliculin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037520">
        <property type="entry name" value="Folliculin/SMCR8_longin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31441:SF2">
        <property type="entry name" value="FOLLICULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31441">
        <property type="entry name" value="FOLLICULIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11704">
        <property type="entry name" value="Folliculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51834">
        <property type="entry name" value="DENN_FLCN_SMCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN FLCN/SMCR8-type" evidence="1">
        <location>
            <begin position="48"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="238" mass="27102" checksum="BEF59F431B36CE53" modified="2022-05-25" version="1">MGSMLISLSHFCDKHGPRILLVTQAAINGSTGDELLVPTYPTDSYCDSCLLHFPKSSASDIRSLKSNVGNQCFVSTQYSSIRYQLLTLIIRRSFSEETMIYDGMPLVFYDDTRGLNLVMGFKLNDDNARGNERRYCLIFTIDSKDHKASMELISANWSFITGGFDKMINYIRDSHRQQIQKKNDTANENGNFSLLGGTYLRGNKVKVPRNLAELTQDELLFVRLHKWNSFLLHTCMQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4L4</accession>
    <name>A0A8J2T4L4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Calcium-channel protein CCH1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">BN860_14928g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="416585at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000093">
        <property type="entry name" value="Calcium channel subunit Cch1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000118">
        <property type="entry name" value="Calcium channel subunit Cch1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF7">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="540"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="647"/>
            <end position="665"/>
        </location>
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        <location>
            <begin position="671"/>
            <end position="699"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="757"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="783"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="817"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="847"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="879"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="955"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1193"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1224"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1266"/>
            <end position="1283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1326"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1436"/>
            <end position="1459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1516"/>
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        </location>
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        <location>
            <begin position="1550"/>
            <end position="1569"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
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            <end position="1599"/>
        </location>
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        <location>
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        </location>
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        <location>
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        </location>
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        <location>
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        </location>
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        </location>
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        <location>
            <begin position="202"/>
            <end position="256"/>
        </location>
    </feature>
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        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
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        <location>
            <begin position="47"/>
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        </location>
    </feature>
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            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
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            <begin position="108"/>
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        </location>
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            <begin position="146"/>
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        </location>
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            <begin position="214"/>
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        </location>
    </feature>
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        <location>
            <begin position="224"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00061395"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00067459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CDF87807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2001" mass="231759" checksum="C8DD8D8B7A9D6BCC" modified="2022-05-25" version="1">MVSTKRRLTQFFEPGTNNGNDQEGTRNNADMNAPLVRPSVNVVPPQTDDEGEDQDEEDDDDDGELDAYSLAERDESKASQRESPTIGDLFRNPSGFKLKRGRPKLTLHTGSSRENSESPANHSRSLSGISADLFRTRGRRRSSGSANTQHNSPDSNVYSDRPPKSARVLSYIAAEDMDEFHDIEKDFQSAMDNQGLTWLPQLGQIKSRSRSDSLDNENEEEGRSDEVLHESVHLSDNEALQPDVGQSKTTDDSKGGLMGLALHSGSHKDDVGSFFDKNSRVEVSSFEVELANFDEPQEEAWQLYGNSLGLISPTNPVRLKLAALQMSEKYRMCTMAILLLFTCLMAYRTYKPASNILFSFSNWADDFNFLLCVLFTLNDVSKIIAFGFWDDSQMFAAYGRPYIPLLERLGITKFYLYLREKYGARIIDTLVPFKLVKDEDEEKFQRKSLSDPLTEDIKNKSKRKHFEIPRAFARSSWNRVDLTSTICFWIALFLTINDYDRRNGIRIFKTLAVLRILRLVNTDTGLSSILRGIKEAIPQLINIGTMLVYFWVLFGILGVQSFHGSLRRQCVWTNPNDPTDKYNYDMQFCGGYLDPTTGKPKKYVFVDGSEGPVSKGFLCPRNSKCVSDSNPYNGKVSFDNIVNAMELVFVIMSANTFTDLMYYTMDSDEMAASLFFIIAIFVLTIWMMNLLIAVLVSSFELANERFKKKKLDMNTIESWPVRTTKGYWKYFQVKAKNKGFPKWATKGLNFFNKIEPVFVILIAVDAILRCLLDATVSERFLRTFFRIDCSISSILFVESVFRLIFHIPNIWKFLTEFSYVYDLIISFLTLLFSALKLNGYLGHTYYWLSILHISRLYRVICSFGFAFKLWKRVLGNGIMIWNLSAFYFFFTFLVAIIMSVFFEGVIPSSEMDDQSFGMFSLPNSFLSLFTIGSTENWTDILYSLQSHSVNISSSFFSSVLLIIWFILSNAVILNIFIALISETMEVKEEDKRPLQIKHYLKYVYPQKIKDYTHATLLKRIRKKFFYNHTNEDSKDFKQFLIRGTAIMNIAQNMGDLANEFRKNNNPSGLPQWLTTTFLKVTEGFSVMQKLKMYADNPFYRNPKVVFNEGEDKNRRTFSLELDEYEDEKLEYLRMHPSFNYTYFILPPRHKFRRFCQRLVPPSVGKRTDGVQFYDDDTDEYGHKSYFRYIERDIFVFFLAVMTLLLIVFSCYVTPLQRMQEHMGIWSWSTYLDIGFICIFSLEFLVKTIADGVLYTPNAYLRNPWNIIDFVVLISMWINFIAYIKNNGNLSRIFRGLTALRALRCLTISNMARQTFNMVVFDGIKKIFEATFISLTLLFPFTIWGMNIFRGRLGTCSDSNLGRSDCYNEFTNTVFQWDIMMPRVYQQPYLYLDSFGSALRSMYEIVSLEGWVDLLENMMNSTGVGTVTSTFASPGNAVFLVFFNFLSMVFILNLFVSFIINNHAKTTGSAYYTIEEKSWLESQKLLSQAKPKAAPSLIELPKSRRFFYSLAVQKNNFYYASFLQLVLYIHIIMLLCRSYKEPRAKTRFEDIYFMISATVFFLQELFHLYGEGFRLYIKSKWNILRFCILLASFVLSAVGFRFSDEQIWFSNIKELFHLVLFLFVIPQSDTLSELAETANASWPPILSLTYTWGILFLVYSIALNQIFGLTRLGPNTSDNINFRTIIKSMILLFRCSFGEGWNYIMDDLTVTEPYCYSSMTGSVTSDCGSKTYAYVLLMSWNIVSMYIFLNMFISLIIGNFSYVYRRGGSNSAIDRREILKYTDAWAKYDTDGTGYLEFSHLPRLMHSFNGPLSFKIWEGRFTVHNLVKNYMKVNPEDPYDVEVDLVGLNNELNSIDIEKLLHRRMQYRKFYQEVLYTNSYWGAIRFSSLLQLLPLYTTYNPRECLGIDEYVRYLYNMGKVEKYLDTERNLDILNMVVASWKYCVKSKKRLSTHSEVIRTQTIEVPSNPFNDDFENDSGEFTFANTPSMDFGVDNFMWSPKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4L5</accession>
    <name>A0A8J2T4L5_ZYGB2</name>
    <protein>
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            <fullName evidence="2">BN860_17370g1_1</fullName>
        </submittedName>
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    </gene>
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        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="4063341at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="328" mass="37623" checksum="D5C215D8C1779C9B" modified="2022-05-25" version="1">MSSGLGRLLKLITTGNGVCRRILTTYAVSAVMFLVRIRRRGQVKKWVNNTGAVAVLFLYPLVLRILSRLGLPSRYCACSITVISSLALAKSIPSWLPSYMMVESVSDYMLSLNALRQLFGGLEESRLLRLRQMIFCLLVPTIRTSPWLRRGTFIRDFILFYCLWCLLSLYQGAKCYIKGKRPTTPQACAKPPQFEESRLLSTSFKPLVDKLAELQELTTCNRQTFAEILMNSALVHNIIPSFKWALWKRFCRSVLSQSNLGRTHLMKSLVLMLGFQVLDQNRQMDISPRVLRYMVRVNLKEFLKPLSNRLQMMLILAFSNLALYNTTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4N4</accession>
    <name>A0A8J2T4N4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">BN860_18030g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_18030g</name>
    </gene>
    <organism evidence="1 2">
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        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="3257538at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019171">
        <property type="term" value="F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026223">
        <property type="entry name" value="Htd2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052741">
        <property type="entry name" value="Mitochondrial_HTD2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28152">
        <property type="entry name" value="HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28152:SF1">
        <property type="entry name" value="HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02096">
        <property type="entry name" value="HTDHYDRTASE2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF87945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="266" mass="31446" checksum="6C314D7B74A1B499" modified="2022-05-25" version="1">MLKNMKWIIPDYVDQLQLKRFTTLIGDRVSINLRSNHLALGDNLLFFNPVQRHLSKDGYLDYQTPSRLTNDNDIMWNRRVWAGGEMQMFKPLQIGQQYYCQEKIKFLKKIGNNYYVGIERSISHSKEQRSLQELRTLVYTSSLVRGVFNEVKTPGKPIGIFKFCDLDIIMYGQLTLNPHRVHWDKNYTQDIEGYKNIIVQGPFAVQVLMAFAESFFAKPLKRVSYKNLNVIYPETEMEICAYHNYIYMRDKYSHDIIYVSAQCFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4T3</accession>
    <name>A0A8J2T4T3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="23">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">BN860_11738g</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 23">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphocholine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38583"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:38584"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(out) + ATP + H2O = a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66036"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:66037"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 22">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="23">Membrane</location>
            <topology evidence="23">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 23">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="23"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070867">
        <property type="term" value="C:mating projection tip membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990531">
        <property type="term" value="C:phospholipid-translocating ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140351">
        <property type="term" value="F:glycosylceramide flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099040">
        <property type="term" value="P:ceramide translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000048">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000108">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF180">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE DNF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 21 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10 22 23" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 21 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 23" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="270"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="577"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="627"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1219"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1247"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1298"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1337"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1364"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1405"/>
            <end position="1424"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="25">
        <location>
            <begin position="214"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="26">
        <location>
            <begin position="1184"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1584"/>
            <end position="1662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="1584"/>
            <end position="1593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="1613"/>
            <end position="1630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="1631"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="20">
        <location>
            <position position="695"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="695"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="695"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="696"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="697"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="697"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="829"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="870"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="899"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="937"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1017"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1018"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1019"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1138"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1158"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1161"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1162"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1162"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="CDF88502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1699" mass="193435" checksum="566028D0B9F0916D" modified="2022-05-25" version="1">MSQNRETRPVYVDETPGSPFEDTFEFEEGTSKGKPSRGGSILKKFGSLRRNNKKNGEDANEDSGVRVTFDEHDDEDASQYLHTPLKHSGAEDMKPGMDDDVHSFDATPKMAEPSATFEDVALDGVDQDKMASETHHEMKRLRMGTRRNKKGTANVGRAKTLRWAKKNFHNPFEDPKKDGPVDETGMQNRVGQLRTVYFNMPLPQNMLDEEGKPNVVYPRNKIRTTKYTPLTFFPKNIMLQFQNFANVYFLVLIILGAFSIFGVTNPGLNAVPLIVIVIITAIKDAFEDSRRTVLDLEVNNTRTYILKGIENPNVSNDSVSPWRRFKKANTKLLFRFITFCRGHMTEEGKRERLQAKRAKLHGTRMSSDKPRNSLDSIDSYRVSADYGRYSQDFDNLGRVVSRTDDVNVIDRALPANSESRFMKDYWKNVRVGDVIRIHNNDEVPADIILLSTSDVDGACYVETKNLDGETNLKVRQSLKCSHKIRNSRDVTRSRFWVESEGPHANLYSYQGNLKWMDSKSDELRNEPITINNMLLRGFTLRNTKWAMGLVIFTGEDTKTMINSGITPTKKSRISRELNFSVLINFLLLFILCFISGVANGVNYDKRNQSRNSFEFGTVAGRPATNGFVSFLVAVILYQSLVPISLYISVEIIKTAQAAFIYGDVLLYNARLDYPCTPKSWNISDDLGQIEYIFSDKTGTLTQNVMEFKKATINGVSYGRAYTEALAGLRKRQGVDTEVEGRREKEEIARDKDTMINELRALSSNSQFNPDDLTFISKEFVRDLQGASGEYQQRCCEHFMLALSLCHSVLVEPSKHDPNKLDLKAQSPDEAALVGTARDVGFSFVGKTKTGLLVEIQGVTKEFRILNILEFNSSRKRMSCIVQIPPTNPGDDPRALLICKGADSVIYSRLKRSGDANNETLLEKTALHLEQYATEGLRTLCLAQRELSWPEYVEWNKKYDVAAAALTNREEQLDEVSDSIERELVLLGGTAIEDRLQDGVPDSISLLAEAGIKLWVLTGDKVETAINIGFSCNLLNNEMELLIVKQAGDDVDEFGSDPFEIVNTLITKYLTEKFSMSGSELELSEAKRQHGLPKGEFGVIIDGDALKMALSNDDIRRKFLLLCKNCKAVLCCRVSPSQKAAVVKLVKEWLSVMTLAIGDGSNDVAMIQSADVGVGIAGEEGRQAVMCSDYAIGQFRYLTRLILVHGRWSYKRLAEMIPEFFYKNVIFTLALFWYGIYNDFDGSYLYEYTYMMFYNLAFTSLPVIFLGILDQDVSDTVSLIVPQLYRVGILRTEWNQTKFLWYMFDGLYQSIICFFFPYLIYHKTMIASNNGLGLEHRYYVGVMVTTIAVISCNCYVLLHQYKWDWFTVMFIAISCLVVFVWTGIWTSSITSGEFWKAGARVYGAPSFWAVFFVGTLFCLLPRFTWDCYNKMFNPKDVDIVREMWSRGDFDHYPQGYDPTDPNRPNISKGGRFGDNVISLGALTNGVGDNYSRDTVMTEEIPMRMMDFEDGSPRQFVKEEAWKASPRENQDLLYSPLLQQQQQQQQQQQEQQQQLHQQQQLQQQQTQQQQLEHLQKLERLEQLAKEEQEQQRKLSEMTAETSASVYGIPRASYDPTRSSYETPRASYHTSRMSYDRTRPSTDRPRHSLAETRTRASLERTRSHMLANNQLDYRYSTDRARTSLDLPGVTHAVDLMNRGKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4Z9</accession>
    <name>A0A8J2T4Z9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BN860_13894g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_13894g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4Z9"/>
    <dbReference type="OrthoDB" id="1588579at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="84"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF88593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="443" mass="49513" checksum="46BC0D0A67195F2A" modified="2022-05-25" version="1">MITTQSTTAKRRLSVHNNLFDPALYKLPQAPQGTFQPRKQALPARPAIFARGEEPLCRASYFKQKFVNLFPKDVQGYLPEVDIKITSICLVWYVTSSISSNLSKTILRDFTHPVALTELQFLVSAMLCVGFMSVVNCLQKRNSQHLLVKACQSFPEGIVPSYLNGNFRESIMGKFLVPCKLVLMTTFPMGIFQFVGHISTHKATSLIPVSLVHSIKALSPIITVGYYRLFEGKQYNTMIYYTLVPLIMGVMVTCWATHGSKAPDSTNTLQMTSGLFFASLSMLIFVSQNIFAKGFLTTRRNRGILPSSSESNLNKRELSPVQLDKITILFYCSCMGFLLTLPPFLTSEFLQHQSVFRDLSVKVGLLIAFHGAAHFLQALLAFQLIGMLSSVNYSVANIMKRIVIILVALIWESKLNIVQLIGLSLTLGGLYGYDKWGSKYPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T514</accession>
    <name>A0A8J2T514_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S03-12266g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_12266g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T514"/>
    <dbReference type="OrthoDB" id="4067709at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF89229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="310" mass="35169" checksum="6A4CAB498222F587" modified="2022-05-25" version="1">MVGFEYSKEAAKLLDFTQKCIENSRSNDDTVERLKIEMKNRRAFRKNLRVQHAIDSGRYDLVDEGSLLQRIPDYPTTADVYYKVDPSSCQVELPSEQLSQKSEAFGQSNKSDLRDHFISRRKLKSHDQDRQKLLLNAQNNTDLIKTLLGNATHFVDLHERTLPPPPPPPPPPPLASALLTESSLRNAASQPQPGNLSQYPPEINQLPYHNNDYPLASHGAPVMLSQNNTSVYYPQLYQVPYFSHPAPIPVAHSTSTLPPPNSQPRNSSHERQKRKPSRTNARVSKMPVKRKVPKSLKRMDSLVKKHDLDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T564</accession>
    <name>A0A8J2T564_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nuclear cap-binding protein complex subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">80 kDa nuclear cap-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_10396g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NCBP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T564"/>
    <dbReference type="OrthoDB" id="10252707at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005846">
        <property type="term" value="C:nuclear cap binding complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000339">
        <property type="term" value="F:RNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000056">
        <property type="entry name" value="Sto1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027159">
        <property type="entry name" value="CBP80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015172">
        <property type="entry name" value="MIF4G-like_typ-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015174">
        <property type="entry name" value="MIF4G-like_typ-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412">
        <property type="entry name" value="CAP BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412:SF2">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09088">
        <property type="entry name" value="MIF4G_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09090">
        <property type="entry name" value="MIF4G_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MIF4G" evidence="13">
        <location>
            <begin position="36"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF88443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="863" mass="99878" checksum="611A72543F6BF5F8" modified="2022-05-25" version="1">MFSKKRRADFEEDDGYRDFKPRTPKRQRVPPVVQLCKEMMPDIRTIGESVKAFEEDIKFLSDAIVNEYGHEDYFNDALLNTFRAVVLEQPHKQPAIALLTMVVNSGNEVAAKSVINFFFEELQKYCASSVDESYEVASNDTGVWNKIKLVLRFLSILSPIILSDDLLGIYRTLFQLAVNLNRDAGPGNRNALSEAIYTNTLLDLPYLFFYNRSDTNLQSKVEELFSFVEKEYEVKSTDINLLKQFNKNAPYQTTELIHVVLPNVKKALANDMEQLSQLFPDWQHILTEQPSEKHGFNDALNLPPLEQLKPFSAFDRGVGSVDSMWREPRYNFHVYLPNSVGDFETVLPITTYAGQLFKDIIIDIVESLEFNRKEVARQVVTLDLFFKTGIFAEPGVSIAQLAATYEENPLASTYKIEDISIETILELVFKLPDVSQPFAYFYTLLVEICQNSPKAIAPVFGRAFRFFYNNLDNLDLELKLRYLDWFSIQMSNFNFSWKWNEWESDSVRFGSCFYNAKMNFAKNLIRKELRLTSNTLDVEDSLPDEFKKYLDSSYFPREKMLEYYQSLFNADSFQVNPEDIKKNDLYFRQESFPAHEKVRQLLDYVHKQNDVRDVSELDAIIDSIRQEFGSIISNFHQFIIVLLVQVVVQSGSRSLSHANKYIGDLKDDLTHVFSKLEMDDVEKQYIIVEAILRFWNSNSQNGFLVADSFKFAGLVSPLSIFQFSFKEQDGKNYGLVDGTSIESTFRNLSQHLDNHDCFAFVSERLVNIVNQCISDLGLQVDEEINIPDVSANPETNIGTDLPQWDLLWKYETTMGFLKSILRKYVDEYRALEENLRNMVETALSHHAPTKSQMVTWMDELHKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T571</accession>
    <name>A0A8J2T571_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_10748g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_10748g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T571"/>
    <dbReference type="OrthoDB" id="4041975at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF88458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="243" mass="28252" checksum="0A745E381FF10866" modified="2022-05-25" version="1">MSWLIDKYLAFEEPLLDRLAHTTYVHHFIHDKISARITLFLMVMGSIAIVNEINITCKMARATKETSEELNKGYIDESLKLHRLIISDDYHSREYLDEKSGIVIEEFEDREKFFAKPVHVAHLYVECNCVETKEGPALLEKPLLFHLEFSPEEYEGEKRPEFGARLRVLRKRLYHLFKDSKLFEELVKDKENFAIGGNVRVYNAQDELLPVSTDEVQLCFLKMETGDTIRCDFLIGEGSLSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T598</accession>
    <name>A0A8J2T598_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome biogenesis protein NSA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_12486g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NSA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T598"/>
    <dbReference type="OrthoDB" id="18388at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22858">
        <property type="entry name" value="Nsa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037379">
        <property type="entry name" value="WDR74/Nsa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16038">
        <property type="entry name" value="NOP SEVEN ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16038:SF4">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="380"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="400"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF88533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="415" mass="47010" checksum="9B0A29B44007BA7F" modified="2022-05-25" version="1">MRLLISCQEGGSLKEAVCAVGTDSSVQNAEQPLKVDPIFSDGADNCVDIMHVSGDNLLLGRHNGLLELLELEGYQSLDHISNLLDDARMEPFYSRSKKRVKLKDCFVAFQPLRDNHFFLATKSGLIHILKIEDNRQLLKIQTLEVIGPLDFAQVCDNWGKQHGKYVFGYGGEENQIKLVEMEADFSKLRQIWEAKNVKNDRLDMRVPVWPTAMKFLQPSSTGSTVDDKVNYQFIVITHWSYLGHYNTQHGRRPLTYKQLFKNREALEHLEVVSPLSTSLGNVATDQFRDFAIITTDTKRNVYKYDINGRLLSKIGRGDISGAASFVKVVEPGCLLEGGLDKYARIYDVCSGERLIKVYAGASIKYITLLDAARAVPSENQYVASKRKPVENREGEEDADELWKELDEKNSKRQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5F3</accession>
    <name>A0A8J2T5F3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">BN860_14246g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_14246g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5F3"/>
    <dbReference type="OrthoDB" id="73691at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF88609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="403" mass="47138" checksum="B807B5943AB9E0AA" modified="2022-05-25" version="1">MLRKKFTQVRKYADLHRVTPISTVSEAAFHDSLQSNRLLSRLREAYLAQRFKVQNEDPSEEINSLHSLPMTVLPRENIFAQVNKDAKIGHWRKPAVKWFRLGLYMLKYYKDGVKNTYILAKRTKPLLQNYNATNCLVSELFKKVEFNEIEHRLMKKSSNDTIKPLPLSRRQFIECHRRKEVWKIPTFFLLALMFEELTAVICYVWPKVAPHNCLTPGGFKKISGAHANEQLDKLKKVPPYASPYMLSEDEVFAKLRSLAIEETPYWKLKLYRFMDNKSIPQEALVKAHQFLFVDDWLLLQHILHEPTTKLTVAELVDCIWLRQLYYRHEDINVMANNTEGRRVLVWRLLIYWAFRFDKTVSCGGDKLFAERWGVNNIALLNYSGKGGGSLVGRDELSILESSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5L4</accession>
    <name>A0A8J2T5L4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ZYBA0S03-07206g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_07206g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5L4"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05474">
        <property type="entry name" value="SAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000023">
        <property type="entry name" value="Aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033876">
        <property type="entry name" value="SAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035217235" evidence="9">
        <location>
            <begin position="20"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="10">
        <location>
            <begin position="77"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="105"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF89014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="532" mass="57062" checksum="917864347A20F612" modified="2022-05-25" version="1">MKFLNVLAYNFLAASLANGATVRHNSVAFDKSPRAVRLSFDKHQARSFEEVLEGKGGLEKRDGDDVSFDLKNELNFYSVALQIGTPGQEVTVLLDTGSSDFWVPSSENPYCKSPSQSGNGTQQEQNEVQEKAQQTLTGGRTATKAGTSQTSATLDCSQYGNFSSNKSSSFRSNNTGFSVAYGDGSYASGTWGTDVVAVGGINMTGVSIGVANVTNSSVGILGIGLKALENTYTGVQNVKNGDTREYANFPMMLKSEGFIDKNVYSLYLNSKDAPQGSILFGAVDHSKYTGQLYTVPILNLYKKKGLKDPLQLQVTLQGVGVQENNNTNTTTTTPVPALLDSGTTLVYLPDEMASLLAEQIGAQWDERIQYYISDCSNLDDAQLFYNFGGFNIASNLSSYIVGHTGQHCVLGIQPSGGDSAILGDMFMEHAYVVFDLERFEISLAQARYDSGQEQVEVVTKKVPSAKKAPGYSSTYSTSQSILPTGNIFTISGSSVSGSSTSMRTKSGDNVAADLAPAPLLALFVHFMLSFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5P5</accession>
    <name>A0A8J2T5P5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_01816g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5P5"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13993">
        <property type="entry name" value="STKc_Pat1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="19"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="315"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="490"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="601"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="763"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="318"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="339"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="389"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="536"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="564"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="639"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="769"/>
            <end position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF88782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="877" mass="98676" checksum="4136F133C73D1E0C" modified="2022-05-25" version="1">MKTLDYEVYKEGGILNDRYQKIEDISEGSYGYVSLAKDVQQKKLVAIKYIFKIEGDDKEDGKEEKNEKTHSLCDEAACEIDILMQLGNEHKNIVQLLDHFDSYIVLEYCSGGDLYEAIKGGLVPRKTKKVTHILKQIIDAVEFVHSKGIYHRDIKPENILISNLFEWTVKLSDWGLATKDATSLDRNVGSERYMAPELFDSNLDIEERKEPYSCKQIDVWSIGIVFLNTVFSKNPFSVANQSDKAFCYFAANREALFDVFSTMSLDFFQVLRYSLTIDPANRDLSKMRTELLNLSEYTLDDEYYNSLDDDLAIVDEPPYVPPSSAPAPVSLPTPASSSNEHKANEKRRASDAKESKVKETNSQDERAKSVPKFKFQKRSHPEAKSIKYNNNNNNNNNNFRNHYRASHNNGAKPKLIKQSRKPLGIPTPNTHINNFFDDYRRGRHNNYGNSNNNSNGNNGGNYHNGFNTRDYFTPPSVHNKYMEGILSSNQAGKRNWHSRPRRSSFSQTAGNGRLALRSPVPANHSPGKYIPPNARASYTNGTDASMTMMHTPTYHEQFAKSDSDGSSSQDVGTGDNDDVLFTLEENDSDFVHDLDNLSLHDSSKKEQIPQELPGPMSSVMPTVHVTSDSPNQGHRRNNNGHYNSNTNNNNNNNGNDNSDGNDLPELLKTPVTEHQHLFSTGNPFSGLLSPRQNNHMDGLGNSAGHRKPSASEQRQKYGVYVPPHHRKSSASKPASVNEATLSVSHNSLNYSTAFQPKQQLSGGYRHNGTHGTQGFRNSSFLPSMNNDHADSTTAVQDGDIFAQTDSDVLLFEDDEAITKSNRPRGRSMFGPYEIYDHTTFPMGKLRTGRKSSTFQEEAVGALEQYKNNWLILQQQQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5V1</accession>
    <name>A0A8J2T5V1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="16">BN860_14048g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_14048g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5V1"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17997">
        <property type="entry name" value="DEXHc_SMARCA1_SMARCA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000082">
        <property type="entry name" value="ISWI chromatin remodeling complex ATPase ISW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000002">
        <property type="entry name" value="ISWI chromatin-remodeling complex ATPase CHR11 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1190">
        <property type="entry name" value="iswi atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.30">
        <property type="entry name" value="ISWI, HAND domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044754">
        <property type="entry name" value="Isw1/2_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015194">
        <property type="entry name" value="ISWI_HAND-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036306">
        <property type="entry name" value="ISWI_HAND-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015195">
        <property type="entry name" value="SLIDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF49">
        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09110">
        <property type="entry name" value="HAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09111">
        <property type="entry name" value="SLIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101224">
        <property type="entry name" value="HAND domain of the nucleosome remodeling ATPase ISWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="78"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="376"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="15">
        <location>
            <begin position="758"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="646"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="935"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="647"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="952"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="969"/>
            <end position="982"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF88600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="982" mass="114816" checksum="5B3997D6E5340857" modified="2022-05-25" version="1">MQKLLKGIDNESKGRRLSDSGSRHTRKSEKEEDAELIADEEEDGVDHYENDDYVTETPWYIKHGKLRDYQIQGLNWLISLHENRLSGILADEMGLGKTLQTISFLGHQRYVKGVEGPFLIIVPKSTLDNWRREFEKWTPEVDVLVLHGDKEERRELLQNRVLQARFDVLISSYEMVIKEKNTLKRVAWQYLVIDEAHRIKNEKSTLSQIIRLFYSRNRLLITGTPLQNNLHELWALLNFLLPDVFGDSDVFDEWFQQNNNEQDQETVVQQLHTVLNPFLLRRVKADVEKSLLPKIETNLYVGMTDMQIHWYKSLLEKDIDAVNGAVGKREGKTRLLNIVMQLRKCCNHPYLFEGAEPGPPYTTDEHLVYNAGKMIVLDKLLKKLKAKGSRVLIFSQMSRLLDILEDYCYLRDYEYCRIDGSTTHEDRIEAIDDFNKPESDKFVFLLTTRAGGLGINLVTADTVVLYDSDWNPQADLQAMDRSHRIGQKKQVHVYRFVTESAIEEKVIERAAQKLRLDQLVIQQGTGKKTANLGNSKDDLIDMIQFGAKDVFEKKSNTISVDDDIDQILQKGDQKTKELNAKYESLGLDDLQKFNDMENQSAYEWNGKNFQKKSGDKTVEWINPSRRERRREQQTYSVDDYYKEIIGSNSKNHSKSNNKSTPQPKTFRPPKLIHGQDFQFFPKELFSLQEKEQLSFKKKVNYKVTVYDITKASEGDEDEEAKLISEDTKKRIKGEQQKIDEAQDFTDEDETRKQELISQAFTNWNKRDLIAFVNACAKYGRDRMDMIRKSVDSKSPDEVEQYAKVFWERYKEINGYEKYLATIENGEKKNEKLKFQEILLRRKIEHCKYPLHEMTLQYPPNNARRTYNSTEDKFILLTISKYGLFADKLYEKLKQEIMASNLFVFDWFIRTRTVHELSKRSNTLLTLLTREFEAPESMKKKRNRVSTREDTPASHNGNETITPSLPASQDEDHIEKKAKLHQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5X5</accession>
    <name>A0A8J2T5X5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_15456g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_15456g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="436637at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="6">
        <location>
            <begin position="43"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="7">
        <location>
            <begin position="460"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="5">
        <location>
            <begin position="938"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="718"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="723"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF88660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1290" mass="145496" checksum="8A561E387C75D8F6" modified="2022-05-25" version="1">MFVDSSELNLYHLTLQRQSNYVHSCIGHFVDGNEEENTTALTNGKIRPKKHLQLCIATETHLELYDVSEGSLKKLALVPVFAIVAAIETLHLENTVCSFLAITSDSGNLTLAKFVRVSDEKVVLKTLLNQPLTRSGLRRLSPISYLQVDLHGRCLFLSAIERNILCFVANQVGQDGICLQDPLEALVPHSLTLDTTVCDVSYDNPCFASLEIRGNHYLVFYVLDLGLNHIVKKADYKLNDTANFLMGLPALSKYHIACHIETDGGGDQVEYDETNPFVLIGFENFLVIKDLQGYFDIKVLIPRRNGKPGPTIIICYAIQTLKKDFFVLLQSNWGDLFKLKIIPDKKNFNRPTATISYFDTIPQASNLHIFKNGYLFATSELNDNYLFQFESLGDDEPLLGSSFEPSVNLQNLSLVEIQKNLNPLSSSQVVNAAPLTFTMSSLNKARFLTNGVNFDTTISTQLPAGADDIWTIKFPTDEYHRLLFLSYSKSTMILKTEEGTMEQLPPDESSFKVKADKTVFVGIMGNKSVIQVCENEMRQITTPSQSNQFERKLEWYPPAGIRVIAAASTRSQLVIALSNGEVAYFEMDTESGSDSLHELQNRIEFNEDITGVAMLPGLRSDFLAVGNKESTIKILSLKKSDVDDFLEVVSIQALMAPVSDLKLIRIQRELQLHVGLENGVYLRSKLNGLDGQLYDVKRKFLGPKRVTLSLIPNVSLTATSKDEESDEDESDGEGQDTLDSEHFKENTRQLKPCVILHSTKTWVCYENDILFHVRPILLSNDNALIKVSDFAAENIKTNGCCALTSSHNLVIGKILDFISKDKWFHVEEVDLNEISNDESRFGQEDEEDLERVPKRLYDACKVITFGDSKDLTLYIENAVDDENVRVSLARAHHFFTLTNNTQKFQILQKMKAVTAVITKFTTSSHYLVVSASDNKLYTFVLTFKRVPNGAGTFELELIHTTQVEDRVHAMIEFKKKLLVPVFGSLILYSLGKKQLLKRSISQTTAFITKITALSNWEDQRIAVGDNRESVTLFCFDDSKNVFVAIADDIVKRYVTCLAFLDQSTVIGGDKFGNIWTLRLPHAYEKLIKTNFPRALDSLQQYRGLKRKAPNAMDCPFKWTILNHFFVNDIPMHIQKIKSVQLSDRPSIIYCGLQGTIGCLIPLLTKQEIMYMKDLEVVMRDADDIFSLDQDTQNTSNMEVTEDNYAFGDDTPKTGDHARTQIPEGAYSIVGRDHLKYRSYYAPVRNVIDGDLCETFVNMSVTEQMSLCKQLTNLKPEDVINQVNEIRTNYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5Y2</accession>
    <name>A0A8J2T5Y2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ZYBA0S03-11540g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_11540g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5Y2"/>
    <dbReference type="OrthoDB" id="2158884at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07830">
        <property type="entry name" value="STKc_MAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="34"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="418"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="664"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="420"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="669"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="718"/>
            <end position="740"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF89201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="780" mass="87944" checksum="3E13443C4152C8A4" modified="2022-05-25" version="1">MLHKKCNKDQTTSTPPDVDNPPLHIPLKSLEERYQVVEELGNGSFGSVALAKAQFDVTTIQNDGGKQHLIRDTLMNQAGSEHDNYNGKQQGIVAIKTMMTKLPALHDYTRVREVKFILSIPANKHLIQIFEMFVDTRAYQLHIVMECMEQNLYQMMRHRRRRVFSIPSLKSILAQILAGIRHIHEQDFFHRDVKPENILISPSTRYFDNEWLSQGHYTDNYVVKLADFGLARHTSNKNPYTAYVSTRWYRSPEILLRSGYYSKPLDVWAFGCVAVEVTIFKPLFPGSNEMDQIWKILEVLGTPHNTKESERTGYESHGGNWEDAKHLAQRLNMKFPYVEGTGLNNLISSSQLQPLTDVIKSCLKWDPNQRVTAQELCMMPFFRGTIAAQECLEASNRKAAIERAAVAAATRVARHYTVDSNSGSSNNSGVCGSSGSSKHGKRTNTEQASIFAGIKSHARAGSTLADKSGYHTRQLIFHSEGFMTVKQQKQRELLPRQDDDDIIVNEQVHEVEDECADDDEISPFRNTLGDYLTDDVAANTPEELVDSDSTISDDGENQDEMDLSHEIAQNIAFCEIPAASQQPFEDSQFVPDGNDYSEGSCHQTGSSVKKLSDEIEAFNRQDPDLYSQYYSTNTMPTAMTTHVTGKSHSHTPFAETLNYVHYDPTHQATTPSSSTGDDTSITHEASTNNNDSKKHQTHSSLQPVNRLLDNMSIDDSFNNNNANNNNNNNNNSNSNSDNINLIPRSFMLPPPNGFEDPIHRQPQPQQQQQHFNHHFGNVTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5Z9</accession>
    <name>A0A8J2T5Z9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ZYBA0S03-03554g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_03554g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5Z9"/>
    <dbReference type="OrthoDB" id="10258692at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000431">
        <property type="entry name" value="Set3C deacetylase complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000494">
        <property type="entry name" value="Snt1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051571">
        <property type="entry name" value="N-CoR_corepressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13992">
        <property type="entry name" value="NUCLEAR RECEPTOR CO-REPRESSOR RELATED NCOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13992:SF39">
        <property type="entry name" value="SMRTER, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SANT" evidence="3">
        <location>
            <begin position="645"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="894"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="556"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="702"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="875"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="999"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1096"/>
            <end position="1123"/>
        </location>
    </feature>
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        <location>
            <begin position="1145"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1182"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="234"/>
        </location>
    </feature>
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        <location>
            <begin position="239"/>
            <end position="269"/>
        </location>
    </feature>
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        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="319"/>
        </location>
    </feature>
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        <location>
            <begin position="335"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="556"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="706"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="737"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1000"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1012"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1026"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1101"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1152"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1193"/>
            <end position="1206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF88859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1244" mass="138141" checksum="D9F3D0BBE172AE8F" modified="2022-05-25" version="1">MPHPPSHRVGDKRRYYYYSSNPRHGGFKKGFQSLSSGPNVPQQPIAASPGTAGLAARHSRYDPHSPRKPMSRVGSRYNPDVEISAVAKSPAAAPSSSITAGVNNNITSTTSTASSPGGGVDGPIMNGSRYSGSRYNPQPRPRSVDSTKKEMQVLTNANSASGYYSRSNKWRMTATAASKLDYDNYGNSQFSRAPFYKSRNRPSLPSSAKTTTPPAVPQHTAVSHHTSVPQSSSLPVIPQTQQTQIQAETQSKKQGQQEQVQAQSPAAPQLKQEGQVQIQPQTQSRQGSQSEVAMTRSPSPTLPPSDDSKLVTTEKRTEETASTETETSTERDTPAATNNSPHSLGASLINSVPQPLTKSQDREDTESEDKMDTITKLSTVNIWSSSPKDRNTDCTHSCPRHEDYEHIYEPAVLKTDLDSLREDSKSNNKLQPLQVMEACIFPMNRAQTRLWQLKSQPRRHITAQQSHLLKNPIKSLQTYPFFRQNIVIHKQALKPLLCRCISKLKRYEYLRKLRLKQALSKVEPQWQENCAKMDTMSHELRKEEICRKEEAELELKERERQEKEVQERQARLSGSSRRRNRADFVDDAEMENVLLQIDPVYKHLQCAAEIPPMIRDPLQKNAIKFKDVNNLVTDKNSWASRILKDGEDSFTQHEHELFVEAYLSYPKKFLKISNYMGGLRSPEECVLHYYRTKSSAAYKKLLNDKNKKRQKSNSAKRSKKRERASDTNNEHSLTLSEHVDHDGKRRESSGKIVDGGRSKVVKHMEQVEIKLIEDQNRLQQVDSDVVKADDAATQEQHIVSKDRAVFKKEENTYETLGTPNARRNSINDSITYSASADAAKGTEVNSAVSSSPSQNGNSSVVKAFTSSADQREVFAESYESSNDEGQQRKRHKHPGEHKSSYWSVKEATFFPELLKQYGSQWSLISEKLGTKSTTMVRNYYQRNAAQFGWKPLVEEADFKRNATSSGSVQQSQILLQSEQTPLSMSNGVPSQLWPALGFFTNGSTKSGNGPNEEPHTDTTADKKTASLPSQATEKSAQPFSSSYNKDSLFPELSSSATGLPAPRLPSIQLPASNEVKKEKAPYTGSQLKDLLNEDCQDDARPSSTWHRSTTDTFGHKSPPSAEHIQLSNTVRPRISNLVVQELQGPIGAPSGSRRSSSLSLLLNPESDGTLKHSALARSVPQQKLAPAQFSEAHPSNAQLQQHNEVSSPPAPASKINFAMDPLGALAAVASESLLQTDGSSKSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T638</accession>
    <name>A0A8J2T638_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BN860_16776g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_16776g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid/choline transporter (ACT) (TC 2.A.3.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T638"/>
    <dbReference type="OrthoDB" id="2417308at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000046">
        <property type="entry name" value="Amino-acid permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004756">
        <property type="entry name" value="AA_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00907">
        <property type="entry name" value="2A0304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF7">
        <property type="entry name" value="CHOLINE TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="421"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="454"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="491"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF87889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="563" mass="62517" checksum="983831F67AAF237F" modified="2022-05-25" version="1">MNSGYEIEQSSQADVHKRDYVANEVPALSFDKDDMDKPNQVEGDGNGEEVHLRKSFSLWSILGIGFGLTNSWYGISASLVTGLSSGGPVLLIYGIILVALVSITIGVSLGELSSAYPHAGGQFFWSLQLAPPGHKRFAAYMCGSFAYAGAVFTSASTTLSVATELVAMYALTHDGFVQQRWHVFVTYEILHFFLMLFNCYGKPLPFIASSSLYISLLSFFTITITVLACSSGHFNSAKFVFATFYNETGWKNGGIAFIVGLINPAWSFSCLDCATHMAFEVEKPERVIPIAIMGTVAIGFLTSFCYVISMFFSIRDFPGLMSNDNGFPVLIIYYQALKNKAGAIVLGCMILFTSFGCVISCHTWQSRLCWSFSRDRGLPFSKWWSTVNSRMGVPLNAHLMSSGWVAIIGLLYLASSTAFNSLITACISFLLLSYSIPVICLLMKKRRIPHGPFWFGKFGLVCNIFLLLWTVFCLVFFSFPPDYPVDRNNMNYVSVVLVGIFLYCLAYWQWWGKKDFHVEGIEDESRDANETIDEYELSGDTQSQGSVLAATPQYIEIQEKSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6B9</accession>
    <name>A0A8J2T6B9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S03-08680g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_08680g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6B9"/>
    <dbReference type="OrthoDB" id="5563539at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007039">
        <property type="term" value="P:protein catabolic process in the vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013860">
        <property type="entry name" value="AreA_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052292">
        <property type="entry name" value="Glucose_repression_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28051">
        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28051:SF1">
        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08550">
        <property type="entry name" value="GATA_AreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitrogen regulatory protein areA GATA-like" evidence="2">
        <location>
            <begin position="200"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="266"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="667"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="994"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="283"/>
        </location>
    </feature>
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        <location>
            <begin position="476"/>
            <end position="489"/>
        </location>
    </feature>
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        <location>
            <begin position="495"/>
            <end position="514"/>
        </location>
    </feature>
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        <location>
            <begin position="713"/>
            <end position="722"/>
        </location>
    </feature>
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        <location>
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        <location>
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        </location>
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        <location>
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            <end position="801"/>
        </location>
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        <location>
            <begin position="816"/>
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            <end position="838"/>
        </location>
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        <location>
            <begin position="1000"/>
            <end position="1019"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1019" mass="111932" checksum="83779277FFEF0108" modified="2022-05-25" version="1">MANNLAAYLSNQRDRSWPLETSSAPATPISSGSINEVDKSSDIKNEDDMGPSVSMAVHADSDADFRKSTFNLKRTRSMGLLDEYIDPTRKLLERHDLGSLPRSRNELMREDEERENPEHLEEQPGSHSEEESSEVSESDSSESSRDDEGGESSRSVSVSPPSADADSLLLPQDDNDLMREPKRHVDYLSHEWNESDISNSWKYIILKKKKRTSVDHVNAARLENASWRTWAKARNHLKTVSPEVVNWSKDSDVTWLYGPIVRGNDNEDNDVERGYGSDDENSKRMSAPRAKKKNSGMAPKPILKKRTVQEILKDNSLWKLSQARKHRNEIMHATSVMDANGSLYDSHDDYDDYDALAARVNAQYYGSAEKKHSNGAGSMAGVPNLSGSHHDAVPQGAEQELLSPVPQNASAKFGGALKTILTSSNDNGTGSKSSKPIRHIHFNDRVEQCVAITNQDSDSDFIDNESQNSEEDHTNSYGTGSIISGRTGSLVQSNEDNDSDTLSSDEGEADDEDNSGLFISPSIPRRADSIIHSPITDSSSTGSLKNSKLSIQPIIKLLPATTLNYGSDDEDSGNSDYNGYGSSVSHNVNTYRGYDYMYDYNSVYTGDTSNFLPVENCDVVDVPAGIDLHTAMAGDNASTYEFNHAALSDLGKDIRADEQAKNFQQNEHEHSFMDNDSDDSGFGSDEQFIEDTKYPSSDEEEDDLRLRRAASIGKTSGTSSFRDLTHGPPSDTQNTPSRTCSFISGKPLEPSLTPSAKSPQSNYASKSSNANEHINHNPNDTNNNDDNNSNIDNNSNNSANSGTIRSRSPRSLKRAASSSFIFNSDSEGDDDDDNDGNDDGGYSRLRKNNLIHENSEAKDNSPYLSFHSRSRTAPPQSSVILPATTTRSVTASGPQLSDLSKSIRIKNSLSSAEVGASDVAIRGSFSPINGSAKSAVSNKHIYDKNKPMSDLKDVDRNLQDYHIDENRSVGASEHRESSESVHKMMQNARHIANKYLHSWKKGEGNSHGTKLDNKDSGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6D9</accession>
    <name>A0A8J2T6D9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ZYBA0S04-05380g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_05380g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="16066at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024372">
        <property type="entry name" value="Ecm29_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055443">
        <property type="entry name" value="HEAT_ECM29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346:SF19">
        <property type="entry name" value="PROTEASOME ADAPTER AND SCAFFOLD PROTEIN ECM29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR GCN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13001">
        <property type="entry name" value="ECM29_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24492">
        <property type="entry name" value="HEAT_ECM29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Proteasome component Ecm29 N-terminal" evidence="5">
        <location>
            <begin position="14"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome adapter and scaffold protein ECM29 HEAT-repeat" evidence="6">
        <location>
            <begin position="1307"/>
            <end position="1468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF89484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1851" mass="207596" checksum="D934FE89F52244D1" modified="2022-05-25" version="1">MESSISEEKEKGLVQKVELRLALADTPEKFEHNLGIFLSPLLLKLSSPYGSVRQAVLGSLKDILARLNSLPKVRIPVDKLLHQAQNVPIDSDQGSANVRLYSLLLASKGVDRMSVNEKKQLIPVVMRGISKLPESAAARIFHILCKLILDWKAPQKGSKEEDEAFEFLNLENSNDLEFLSKKFTKFFFLIPARPAPDTGVIARGYTCPGLSAADVSFFTYIAGVSFNKDQLLRFKSAIFRFFNGGFTVEARLLAEFLVVSSTDDSDISDTATFYLKRLTISVENHDFIDYLVSLYIGDKKIGTPPVKYELQEKILSYLNKSVYATADAKRVSQICHMGLNSPHYKLRSLSLIFIRHVAKHNYRALIEQTHEREDVGFNINVASLIKNNLHSEGWPRLQLSSTTPNFANSLSQRRLQYETLGEILSKDFDLIKDFSYIEFLLDSLKGDLPDCWPSILEALNCLTSSLAKLPQCSKEKLKTVVRESLSDDFELESTESKERKDAIMACRFVLIKYTNAAFDFVDADARLFNVWGTSRINRFDIIEESYKGLNPYWYNINRASNTKEFKPTGELLASKITEIELPSFENFVNLLLKKTDPNSLAVTSAIRSTLGTAIRFSKQCLISQSAYGKATCVTQDEDWSLRIEKALDVDEKVPGLLLGLISRFKGDWFARFLKLLCAEVIYKDETGKQISISKYPDAIFGEFMLLLLGNCNDKTLAALGDLVPGLYNYLRTVETANDLDIATGANLLGIISASLPESIHVRSIITSSNEVVNASDKINPSTIANSYIFPRLYLRGNFQCIGVEKLESLLGVLLPIISVPRLKTLVAKLLCQLLKFGLILELHAQTRRAIILKIMNILSPKLQKDESLMELWGFLSLYSKESGFFDEIFSKLYDTHATKQIELLFTGGEAFSILAGGWKSAYLAKQLDISRCVLGSLQHTFSDDYVSVVLDRILLACDSTKPSLRKSACIWLLSLVQYLKKSSRVISRCNEIHLRFMKFLADRDEFVQDSASRGLSLVYEIGNGDLQESMVKSLLKSFTDSANAMSMSSGSLSGETQLFEEGTLSTGEGSVSTYKDILNLASEVGDPSLVYKFMSLAKSSSLWSSRKGIAFGLGAIMSKSSLENLLLQDRSISEKLIPKLYRYKFDPYQSVSRSMNDIWNTLISNSNSIINEYFNEILQELLTGMGNKEWRVREASTLALLNLIQTQSDASLDDKLLDIWFMAFRSMDDIKESIREAGSKLTTVLAKMLARTVDVNKGVKPEKSRRILESIIPFLLGSKGLNSDAEEVRSFSLKTVVDVVKNSGSAMKPFAVDLVFEFILLFSSIEPQVVNFLSLNANTYKVDANTIDMHRKSAVTGSPLLQAIEKLIIDSDDSQMEAYVGSVTKAIKKSVGLPSKVAASTVLNVLVKRYSFSLKPFSGKLLKICMNQFEDRNESVRLAFAVSFGHVYHVASLEKCVKYAEQLSTRYFNSVDASSRKVVGYAIEAVSLHAAPQFEDTAGIYVPLLFVASNDADESIRAFFTKIWTESSSSDIGAVKLYLEEIVDLLEKNIGSNDFAMRRTCAKSVCLLCDKVDATVPAKVLAQLFSITIEALKGRTWEGKEVIVEAMVALAVKFRDYVSTHSELKDSIGEVLNMEISRQNKKYARQIVFCYCQYLDAFPSLPLIEKLIQVGQSFFDVPELDGSNKRSKPFPSEISVKSSKGNIEREDHSIKFLKACASICKPIDSIFPYHLAQFVMDKVIFLFQEDYVVYTWRTQVGACEIGLKFAENYESKYSTPEYETNLRIFWHELFVYNSTKETIENAKVQLIRLGGKWMNKDAQLKDEIQGDLIRLSEAEVSSKLYNELSNLGITH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6H9</accession>
    <name>A0A8J2T6H9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S04-07228g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_07228g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6H9"/>
    <dbReference type="OrthoDB" id="5589766at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040206">
        <property type="entry name" value="Zds1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013941">
        <property type="entry name" value="ZDS1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28089">
        <property type="entry name" value="PROTEIN ZDS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28089:SF1">
        <property type="entry name" value="PROTEIN ZDS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08632">
        <property type="entry name" value="Zds_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01327">
        <property type="entry name" value="Zds_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein Zds1 C-terminal" evidence="2">
        <location>
            <begin position="1024"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="564"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="664"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="772"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="819"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="898"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1085"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="317"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="668"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="699"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="773"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="910"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1104"/>
            <end position="1117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1126" mass="124968" checksum="32D5D056E4AAF71D" modified="2022-05-25" version="1">MDVDQSRILERQQQRQQSRPRKSENMDEAMREKRKSEVMIAAQSLDSEVQSVKNLKRISIGSMDMLMDPELEYRVSSTVGKQDGRKSWTSGLPTRPADETENVHNRAAVRYTYPSRAVEGGPTDPDASLESDLSEISFDITNSAYLEETGADDSSSLLEGDSTDPRGSQKSQVSSSGRSISGVGSLKRGVSNVALRKSSTSSDSSTGSNPSLTKNLLWVPASQHPNVKPENYLELVQDTLHNIKIDGNETGQGSGEVTDNSTNKENEEQTYRLRKNSSLVRKPSRLRKSYTEFDDENEDYQDASAYSKKRPSDDLENTSSQTVSSANSSRAVSLRDITDELTKISNNAGLTDSDAITLARTLSMAGSYRSDESDDIIDDSRVEYGEELNYGGEATVGERRQEESEFASNMIMKNGFVIPARSSLRRSKFNTYRVRSGSDESLNEESQLFESESNNRQTTQGQVKETVTNDDAIDQSLQSGLREGEVVVVERVSAPVGADDTQQSKQQEHLGTPVDQHSHDSKSPSKFIGEDGSNLTSPSTISDFQDIYDHYRQSSVDWEKELKNEQEFSQRDNHNKPATSNVLSEDPTKEITIEVGDASMDMSAENTVMSNNSSVSNADLSADPSIKPENVKLLDNAAIGQQPPITHSPAKREHSMVAEKPRLDHRASGNSDNENNSGNNRQAQKRGGWAIFNMGTKDFLSDRKEERNEPTKERETSHKEGYASGNGDFIKTSSHMRTEFERLAADRSNHSKNRHSPIFAEANKKEIFEESNSDGITDTEPSAQVSEDQSHIDSANIDSSNTFSAQRLEQKFVNLFKRKGKGKASSSKTGRNHKRLELTKKSSNNSLPKFRRTTRNQPKQVEEEIEIEQNEAAMAISTSEITIGEPADDALGTSISTSYEETPEEKQEQHLSLQPAVSVQSTKSSPTIVVESVKELETEELQDSGMETDGDTQKIPQETIKMKKQPSGERAKLAMQSTQPTVHAKPQPLPPRKLTFSDVTRLDRPNAPMRFTDSAFGFPLPMLTISTVIMFDQRLPINVERAIYRLSHLKLSDPKRELRQQVMLSNFMYAYLNLVNHTLYMEQAAQERGHEGPETGANEADASRSGSGAYTTDQNDANGAICIPDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6K1</accession>
    <name>A0A8J2T6K1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Stress response protein NST1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_08284g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May act as a negative regulator of salt tolerance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NST1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="21629at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025279">
        <property type="entry name" value="NST1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13945">
        <property type="entry name" value="NST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="174"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="254"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="554"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="661"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="758"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1122"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="182"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="195"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="266"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="305"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="560"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="595"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="661"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="680"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="758"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1122"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1155"/>
            <end position="1174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU049441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF89605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1392" mass="158269" checksum="3ADCDEF9D7D73ECB" modified="2022-05-25" version="1">MPPSSRKGKKKSKSKQNGKKAVATSQSDGHGLIDAELYHEDGDYPSSRVIKRAPNGDVIVESLPVDDKRKDIGHTDDNLQQSPMAFTLDSHWESLTSEEKKNILRIEKNEVFDVIRKYQNNHSCNCSVCGRRHMAMDQEMERIYNTLYELDKDRDPEINPVKFHLSIIKELQDSKSQQQHQVHREQQKKIEQQEQEQQQQVHQQQQVQLHNLPELEKSNRQENSQTDYENMRDEVVKFFLSSNAVDSLKEEVMHFKESKQRQQQHPQHHRQQPHQQHKQHHQQRQQHLHKHHHQRQHERDHENANHQTLQEGQKLQDNPTNQADNQPDTVRLPIEEHTGNSHLLTPVAYPNAAYDTDNPSPSVDAEGLQGKYLNFAKTFVSSHPKIAQEYVNRMMMYPDMRELTDDLMNNNGQGFIQAVEGFVLEQQSQKDPNQLQQLIGPNDADITQYQHLGNAKEFTTMLHNGTPLTEEEYINLQRQIAQRMTNCYDTQKKEFKEVSQLERELFIRFMFGEDRKSFGDLIMQAFKEKFNDDYGTAAIGASLAAAAAATSASMKIPGSDETDADYDYDDPNDDGGAIDSGEYDDHFDDYSGYDHEEDEDFENEEEYDVRSDLNEENSHGMTCDCDDDGDQVCSHSSHEEANCKDYGHVCGKSELRDDVHNHRHHHHHHHHHHQHVHEDYAEEGEDEEDEDDDEEDEEEEEYDSGLDESDRLEEGRKLIQIAITKLLQGRIMESYHEKQAENNRLKLLQELEAEELKKKAKEEKKQKKREKEREKKRLQQQAKEEEIRKRDEEAKAAKKEAEEREKQRREAQRKKVEETKRKKDEERRRKLEEQRRREEEQERQRKLKEEQKRKRDEEKRLKEEERRLREEEKRRKEEEKRKKEEIKKKETDIKQNEEDKKNRQNQMRGGLPQELAKNTGGSLLGSAKNNPQSISTMLPENAYGDTRHTRSNATLDNNINQEFFSLVNAASNSRTSSSMGEFHKLMQIGNGISNQEEPNKSILPGSSLFGSSNLLPSLDALSNIEGQISPVQPPAAGLNNQAYNISSWDGFLNTGITNDSSGGLRSSQQVQQAENQNLLQQQKVQEKQYCSIAPSLGLSSSDPNEGNTFEEEMNSLTSMLTSAGLGSSRTTHSFSQTPLWGSGAPQNSMNMVRNGASAAGTQVAADATTGATAGPRSSIWEAPIGGPDVTTMANGIDSATSQFPASNIWSPTAAPSSVSFLDSHNVAPSNGNQLGNATKLDPAGSSFLMGNIYNTYSAFAAAQDPSLREHYVSANLLHQNSVCDSMDYSTFVNYLLNMRSSHGCELLTNETGTVTHTKMVLGLPQSSLSQFSGNVTNTMYPTSSDAKPMNAALSQGETLPQSGSLGSGLKPNAQLYNIGQRPNASLVSNIWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6R9</accession>
    <name>A0A8J2T6R9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GTPase-activating protein GYP5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_04280g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GYP5 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6R9"/>
    <dbReference type="OrthoDB" id="295078at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000044">
        <property type="entry name" value="GTPase-activating protein GYP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23436">
        <property type="entry name" value="RabGap-TBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="13">
        <location>
            <begin position="701"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="639"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="928"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1067"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1123"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="996"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="116"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="229"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="244"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="289"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="321"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="353"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="387"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="448"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="516"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="928"/>
            <end position="945"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF88187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1156" mass="129631" checksum="15B715672B47D4D8" modified="2022-05-25" version="1">MGKKKGKKSAAFKAQRAAETLDDNENQNSTTDANDASEAPPATGDSLDDLNATTEELNSHENDVTPKETYKQEHLTILNPEPASGDIVDVSAHSSPEVKPLFADEFDNSLLHPHQDSQSDTVPVTDSLQTDSELVNTANPSETQPLRIRKPDTPIAESPSQEHGSSQPSPHQNSEPNIENSHGDFNPAVGAAAEVENEDHPTRVTSPRPEPPSLDATALGPSEVLEAPSQENNTSQPNSPQRSTRTDIKNTHDVHDSNVESPAEVEADDRSIRATSPRPKPPPVDTTPLNISYVANSVKSSSPRPLPPLPNAALTEVSPIASGSISGLSNHTPVTETSNTESQETGKASKPTSPRPLPPIPVDLPVTKSDDLQETKQPNKPPLPARSGQSAFSSRSTQITPLRASYEGNSADEEDDEVPGLRSFHRTSQRSSNRSVVVGEPVKSAPPKHFEPLRTEATDPTSSEMTDVDLNELTEDNRESLATPVRLTKSKGLSRNHDEENQLSTPTRKLDPSVAPSETSHISPKTLRPETQSPISPPLPARGTSMRRYQVPPPLEQEMQSEEFRKNLSLTRVRDNEPKPPPINRAKRLESAAEINLIANRFRETTQYYQTEDEVSRENIEKGQSALKSSFSTFLESLPGTPLTPGGKFEGAQATPEKESDLGDDELNLLKTDWSFWTQVVNDFPSVAGNPEILEERITEGIPEQVRGIIWQLIANSKSVEFEDIYQTLRDTASPHESSIRRDMKRTKFIPERKFESLFNVIKVYSVFDPDVGYTQGMAFLVTPLLLNTETEAEAFGLLVRLMKNYGLRNMYLPEMPGLMLMLYQFDRLLEEHSPQLYNHLTRQGVRSSMYATQWFLTFFAYKFPLGFVLRIYDIVFVEGVESFLKFAVNLMLKNIDNLLDLQFDRLLDFLKEELFYYYLKTHVEQRKNGKNEDNDNDSVLKREITSTSSSSKRADIQDDEYDIDQFVWDAMNDVHITPISLRRYTSEYDEIHEIEQQKEAQYESIRIKNQQLQKEVRKLEHGYTLLNREHVDIANELIKNRLKIETLNDENNDMKSTILDLKRQLEERDRKQASPSPDDGLLPSDIRQDLNRTIKRNAEVMSENLKLQDKITELEKTLATLRTKNAAPAPASGVSSHVPREKLSNGWSGLKKVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6S6</accession>
    <name>A0A8J2T6S6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S04-02696g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_02696g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6S6"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF89372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="645" mass="74690" checksum="99147C300C9D3804" modified="2022-05-25" version="1">MFRARGLALIRTFHSCQREQGSSLKEKLRNVQQHDILMNKSSKELAKLDAKKRIQFKKLQKSAYPRQQALHLLKQKHGDLDEEIKAAVLGPTSQDDLKHLIHTKDKRMIYTILGVSGDQLRDSKLIAGDVKKFLRRGQVEKAVFLSRLAKKKGSAAMNAVMEYYFNEKQAPQSGIDMYNWRKKWGIPSNEFTNTILFNGLAQQKRHVSKASGDLVLKTIDRLVKDNELSQIEYNAAISALSNCTDVTAAFELYERKIKGISKDAITHLWILRACKRIASDVLFQELVLTVLRKIPASCIDAQLLFEFCKTLNSRADCGRLQSMTILALNEYFEIELDQKSFPKPIKDFHFVPLSHWSIRERYPLSMSVLGLLLDNCLQTGQYELGIHFFRQVSKHKRDMLDIDMFHKYMELLIRGHSSTCGDQCLEVFNEIENDSNLPTVKHSLVLLYRAFEKQARKKVNNSERDKIDKFLKKCLCFIKEVEGVYSKEFNDRVYPAKSWKSIFSILKAANAHEAVGNRCLVAILDDYLKSSIRGEFEQFKSDSPSAQRFVELELVRLLNQIALRMALPQIENVDHSLPSTERTIFLQRRRLLQLRNCVLKHVEALESENRNKDVLEKWELALREKARHIILDHATEEFNKYVLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6V0</accession>
    <name>A0A8J2T6V0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S05-05864g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_05864g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF89968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6V0"/>
    <dbReference type="OrthoDB" id="4068356at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="774"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="265"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="635"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="687"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="775"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="827" mass="95143" checksum="2CA4D5255394C6CC" modified="2022-05-25" version="1">MNCLTYKATSSNQPRITAVKLESTEIPIAPQKESSKKNRQANTVNLPTLKDRNAHLSSNKVPTPLKESIDRVIKKDTVQNQVTRNPIVTPTHGVKNTNFIIEEAIEGFEFLYSNAKPKGNLRFGENRTIIFDKEEEVDRLVTQGIYVRTNGSNCQSSNLKPVLKNRLVNIDTSYFEQEEASYLEDDSEEAITENTPLINDSTDYIVIETRQTIERLFTLHDIPLDEVEDDLLGSLRRLAAGVVETFRKKERCYKYVGRQQSIEEIDELEYFNDNLRCEVSKLNNKLQEESIKWEVVDQTFAENDDLKAQISELTLATQRLAKELELSPKWEDVDGLLEEDKQLKEKIAELTSSNSKLSDSCDSMQILALKHTEELKEYKTDIEQLKNKISSIQKENAELSSLNEKLVSTNSAICGTIDKLREENVKVLKKMKSAKKKAYSHYFVENITLTAMHEDLAHKLDDVSIELDCVRKANKELLLANANLLFEKKELSSAGSQLSSTVNDLFERVKNITMECKDWQNKYLKSKDNYISAKSDMGFIEKLYDYEIKTLGGEKTALEKKLSELRLLSEQSTLKAALFSELYNSVCEDKETISKELDQSLIEIKILRSQLIDSEASVMHAQQWQAREEICLSSLNQIQDEKAKLLEKYSKLEIRFTQLQQENDELSSFKKDYRVFDEKYDWLESVALSLRQEKEHSDDQVRELKRQLRASYKTIESLENAKLQSQKKPQELQKQLHELQTKYSQLVGDFTTKETELQQALCRMQELATMLRGTMGQTTTSSPTEWEPWSAEKEEAPATSKRWQFRRSASLTLGRGKKGFFPRSVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6X9</accession>
    <name>A0A8J2T6X9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ZYBA0S05-07294g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_07294g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6X9"/>
    <dbReference type="OrthoDB" id="1926336at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051952">
        <property type="entry name" value="Golgi-autophagy_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000237">
        <property type="entry name" value="GRIP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23157">
        <property type="entry name" value="GRIP AND COILED-COIL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23157:SF25">
        <property type="entry name" value="GRIP AND COILED-COIL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01465">
        <property type="entry name" value="GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00755">
        <property type="entry name" value="Grip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50913">
        <property type="entry name" value="GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GRIP" evidence="8">
        <location>
            <begin position="860"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="118"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="247"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="762"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="812"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="305"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="534"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="597"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="271"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="765"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="816"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="846"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF90030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="910" mass="103899" checksum="48DC63C53C192839" modified="2022-05-25" version="1">MFKQLSQISKNLSDEFARGVSEDLNPPQEQQHDDSGLPPDVLAKLRKFEKYEDKYPKLLAAYKGERAKLEKAAEIEKVLTEYTPISSFDDAESLAAFFKNINEKQQLLNNEIKRLITSGTQEEDNTQNSGQNNGDRSDGERNYEKEIEDLKNEISESNKKYNSKITELTEKSVRAEETIEKLNEEHNSSSQQSKDLLEKKLLENSTLQEKIDEMERELTSKKNSLKELQEQFDEQKRKLDVLEKQSVEGQLPTGNAGTTSSILPPTPTSKSSKKKKKNKKKLAAATQVQSSSPVASASCEDAGEMASLREKYNDVCAKLETLKESLDAEWQGRYDTLRAELEASSSSNGEYKQRLEVMEKECDNLKLELEKSRDLLKEKESEIEETKDMMKTIGNELVDAKDQLKEYSGSNKEDITYLNNQLEKVSSELDSATAKLKQKENEFNGAVKNLNKEIAHLKLTSSSNIEQRKQLEMNNSKLAETVNKLKEEITALNRQLKDLENLKKSHASLKTSIIQKEKTVIYLEEQVAKCTETIEATKKSSETAKRENKQLSELLEHQKKEFSHLQNDAKSKTESFEKYIKENGQLSERLLMLQSRYDNLQSLKSNSNEQIDSIKRQCEELTFKLKEANKRILSLEDEVSEYSAIIRDKTREANSMRRLISETTNGESAKERELIERLTYETDEKIKLQNELTLQATRKARDIQDLKQANSELKSELHSLQLSEKELSADVAALRRLNANLQDRHGSTSEDSSELEKLAAGLKESLSKSDQKIRDLQETNSNLMRTNDDVNEKLNRLSKNYKALSKQLAELRESNRNGNLTRSSRSSSVVSNNENLNSTNIDSRHSSFSSNRTALPTETASETNEKVAYIKNVLLGFLEHKEQRNQLLPVVSMLLRFDSNDEKRLMMSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7L3</accession>
    <name>A0A8J2T7L3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BN860_15742g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_15742g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7L3"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="5">
        <location>
            <begin position="457"/>
            <end position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF88672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="913" mass="106216" checksum="B70D96F24D991600" modified="2022-05-25" version="1">MLRLPLKVCNRWVQTHRSVLQKVTLLLIVVLVVARQAGWVSTPAAVQLLVAMGPMAGRARTLYRKFKFDTVMQWPEEYTLRGSLLTPSMGPLKGQELGSIDDLHFYDGDPRLVWSVYLDHAMNAVQSYQHEAANSAEHEQPPTLPFSWYDWADHHDFNKLIAASQDGGVEITCPFLYERAFEAEKLELIEKVAGSPLFSYQRVKYYDPEWYKKIRAERPGPEKVPETQCRRVNEAAAVKFNLPIQVHELHEDVRPEVYQMQARSYLFSELRNPLSLTMLESDRAAYRFPVRPTRSNLVESRMLEAFLSRHMTRADEQADFTFDHVARMQEFLKSDAAFKAKVVIPDAHRTQEDESVIPLKPSDFEFDTRGKIKELEKLEADKKINAHQKQYLESLKVSIATHPALAPKYLSEAAHISQFAGMGYHRDKRFFNGGLVEEYQEYQARLNSLIRTWLRFTRTNGLLTWVAHGSLYGYLYNAKNFPWDNDYDVQMPIRHLHLLSEYFNQSLVLEDPREGNGKFLVDVGTSITVRTNGNDQNNIDARFVDIDSGLYVDITGLSVSSNPQRDNFKDWYEQKKKTINMEQDMKEFKNPEVGDGLAAMDINTLAQYAKDHDSEFNKEQKEWIKKVAKSEKDNFEKKNAPQKGLTPPQRYFMNREVGAYNCRNNHFITMDFASPLVKTLYHGVEALVPYRVIEPLRKEYYVPKDLGFTTYQGNVYVPELRSWFNLNILKRFANMRSWNANLREVKSPINDLKLEDLDPLFENMHNIGYTDLFSLYYTSYNITAYRLKELEIQYDTTLTKEDKEDVLEILRHDVAPKMPPPVKDPTLYMYEKRLWEDLAQSMTKGEVDRVTQEVSYGIYKELFAAATDLYNKKASLFMITDDSGEPYDFNHIGTEIPKENKLFEKDYPDDRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7Q4</accession>
    <name>A0A8J2T7Q4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ZYBA0S05-07624g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_07624g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="443634at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15482">
        <property type="entry name" value="Sialidase_non-viral"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.270:FF:000005">
        <property type="entry name" value="Sortilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031777">
        <property type="entry name" value="Sortilin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031778">
        <property type="entry name" value="Sortilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006581">
        <property type="entry name" value="VPS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050310">
        <property type="entry name" value="VPS10-sortilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106">
        <property type="entry name" value="SORTILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106:SF27">
        <property type="entry name" value="SORTILIN-RELATED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15902">
        <property type="entry name" value="Sortilin-Vps10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15901">
        <property type="entry name" value="Sortilin_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00602">
        <property type="entry name" value="VPS10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110296">
        <property type="entry name" value="Oligoxyloglucan reducing end-specific cellobiohydrolase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035289748" evidence="9">
        <location>
            <begin position="20"/>
            <end position="1580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1375"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="domain" description="VPS10" evidence="10">
        <location>
            <begin position="43"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="VPS10" evidence="10">
        <location>
            <begin position="725"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1523"/>
            <end position="1580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1524"/>
            <end position="1534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1547"/>
            <end position="1565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF90041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1580" mass="177008" checksum="F5CF0327D7EF5C3C" modified="2022-05-25" version="1">MLIRLLLSLWACLAAIGNAADFKPKVTRSSRPVIYSVKAFDDSTTLLMVQKKGLFVSFDNGGKWEAPEGVPAEVIMVDVDEYHRHDRAFAVTNDFQYYLTEDQGKHWRHIQPPKGSNDFTFCELDSHPLNRDYFLLRCSDCSTVSLPGLGEFSKRAMFIPSCKEESYVSSNGGKSFRKLESPQHPSQSGSQYLGVICKFGVNSADSKLKSGANFIYCLEGQMDEWFQTNGVLFYTSDFGKTTKMVDQFKDVTVSDFQVLDSHILAYTLEDRFNRLSAAQLWVSSDGFNFKKAYLPTLLRYTSLYDATEDSLGRIVLPLSTMMEESNSASQILLSDSSGLKFSILQVTPEDILGFSTIATSPNLRGTMWADFMSITNRPFRHAEKDKPNHLSKVTFDSGATWSNLKVVDPTNRHKKLFHCDINDVEHCSLHKFFGDPVPIGSAGILMTLGTIGDGTESMWEDIMTFISRDGGATWEVAFEYPCAYAFGDLGNIILAMPIYPDEDNDPQAEFYYSLDQGKNWEEYQLEVPITPMDLLSTTPDGSGLNFIISSFNLGNSPNNGDADSFFYTIDFSEAFEGKICGNNDFEPWYLANGDCVNGAKYSYRRRKQDSRCLVRKLFEDLTLKEEICEQCTEKDYECSFEFSRDSDGQCKPDYKLLGASSICDNSKGEIELKPMQKVTNSKCRKELKVESTKVSCAAKSDILITENVFHSKIRSYQYFDTNEDESLIIDTVKDGVYISHDSGQNIKKFEPGEDITEIVFNSFHSSHAYLFGKNGNLYVTRNRGKSFETYKLPYSRQLGMPLDFHAKDANTFIYYGGKDCENIFNPACHAVAYFTRDGGKTFVELLDNAIHCEFAGSLFKHPVDESLIYCQVKEPQSRKRTLMSSVDFFQTDKKTVLDTIIGYMNTGEFIVMAVPLGNSELRAYVTVDGAELAEAKFPPDMSAEKQEAFTVLGSETGSIFFHLTTNDEPGGEFGELLKSNSNGTSFVRLESAVNRNSLGMVDFERIQALEGIIIINVVDNPERVLAKEEDKLLKSKITFNDGSDWSYIQAPLQDSERKPYACNPKQLEKCSLHLHGYTERNDLRDTLSSSSAFGMLFGIGNVGEYLLPEDQCSTFFSKDGGETWTEIKKGAYQWEFGDHGGVLVLVPKNEATNTLTYSLGMGDTWSGGSTFSDYVFTDEKILVQDIVTVPRDSALRFLLVGSSTSVQGISTKTFTIDFTQKFKRQCIINGEKPDGADFSYFSLGESDSKCLFGHQARYLMKTHSECFVGNAPLSEFYEITQNCSCTRNDFECDYNFYKAYDGTCKLVEGLSPADSSEICKKEPDLIEYFKPTGYRKIPLSTCVGGLRLDDTSAPLPCPGKEKQFKEKYKVNRTPYFLSFLFFFLLLLAITWFIYDRGIRRNGGFARFGEIRLDGDDLIENNGTDKLVNSIVRSGFYAASAVFSGYQLLKRSLGNTALRISERFSRRRGPTYSSLLHDQFMDEADDLLTGHDEDANTLGSILGNDENFEIDDDDVVFPADHTTLSEDISASSPVSPTADAADLARNSPPATAQEQAPTLTEGSANDINHENTDNEQDLGVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8C3</accession>
    <name>A0A8J2T8C3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetyl-coenzyme A synthetase</fullName>
            <ecNumber evidence="5">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_05776g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23176"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="5"/>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T8C3"/>
    <dbReference type="OrthoDB" id="1706066at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05966">
        <property type="entry name" value="ACS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000004">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000001">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF14">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="8">
        <location>
            <begin position="88"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="6">
        <location>
            <begin position="151"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="7">
        <location>
            <begin position="602"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF90642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="725" mass="80368" checksum="10503ED6032F5625" modified="2022-05-25" version="1">MSPSTAPAVKPDAGDSSSIIGELKSKLKAVAGSERSDEVAEMEQEQEQATEYEHLTRVPIVKQRPISDRLQPELAQHYSPHLSGLQEYKQLYKQSIESPGKFFGDKARQFLNWFKDFDSVFVPNPETGKPSLENNAWFLNGQLNAAYNCVDRHALQTPNKPAIIYEADEPGQGYTLTYAELLEQVCKVAQVLQYSMGVRKGDTVAVYMPMIPQTLITLMAITRIGAIHSVVFAGFSCNSLRDRINDADSRVVITTDESKRGGKIVETKRIVDDAIKETSGVRNVLVYKRTNNPKVQLVPGRDLDWDDEIKKYKGYCPCEPVDSEHPLFLLYTSGSTGTPKGVQHSTAGYLLGALLTMRYTFDTHREDVFFTAGDVGWITGHTYVVYGPLLYGCTTLVFEGTPAYPNYSRYWDIVDKYKVTQFYVAPTALRLLKRAGDSYVENHSLKSLRCLGTVGEPIAAEVWDWYSEQIGKNEIPIVDTYWQTESGSHMLTPLAGGVTPMKPGSACFPFFGIEPAILDANTGKEITASHAEGVLAVKSGWPSFARTIWKNHDRFLDTYLKPFKGYYFAGDGAARDKDGYMWILGRVDDVVNVSGHRLSTAEIESAILDDSIVAECAVVGFNDDLTGQAVAAFVVLKNKSSWSTASESELQDIKKHLILAVRKDIGPFAAPKLIVLVDDLPKTRSGKIMRRILRKILAGESDQLGDVSTLSNPGIVRHLIDSVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8H1</accession>
    <name>A0A8J2T8H1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S05-08372g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_08372g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T8H1"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005946">
        <property type="term" value="C:alpha,alpha-trehalose-phosphate synthase complex (UDP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000131">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1020">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase related protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF123">
        <property type="entry name" value="TREHALOSE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF90073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="891" mass="102010" checksum="2F241451D63F1CFE" modified="2022-05-25" version="1">MAPTSPKRQRIINCVTQLPYNIELGKTNNEWTISPATGSSSLYSSLEYLNSEEKSGEYEQHVLGWTGEVTRKNTGRFVSKRNDNEVIEDEAEEDPLYLTADQIKGITADLHKQHEKYNQYNVANVHPVWLLRRDQERWRGFAENVLWPTFHYILQFSPDGSEKENMYWYDYVKFNEAYAMKIGEIYQKGDIIWIHDYYLLLLPQLLRMKFNDQDLTIAYYHHSPWPSNEYFRVLPRRKQILDGLMGADRVCFQNEGFSRHFVSSCKRLLDSTSKKSENRLGMDQYQISAYGGDIVVDSLPIGVNKNKLLEETFTKDIDKKVLSIKEAFQGKKIIVGRDRLDSVHGVVQKLRAFETFLGMYPEWRDQVVLVQVSVPSKKGGADQVIRLEQQVNELVSSINFQYGSLNFSPVQHYYMRIPKDVYLSLMRAADLCIIASVRDGTNTTAMEYVTIKSHLSEYNCYGNPLILSEFSGISTVLKDAIIINPWDSVAVAKSINKALHLSREERTSLENRLSPEVPTIQGWTDKFLLTLKDITNEAQGSSDRRITPALNRPVLLENYRQAKRRLFLFDYDGTLTPIVQDPAAAIPSARLISILQKLAADSRNQIWIISGRDKKFLNKWLGSRLPQLGLSAEHGCFVKDVDDEHWVNLTEKFDMSWQAKVGKTMEEITNRTPGSVIERKQVALTWHYRRADPEQGEFYASELKKELAGLAEKYDLDIMAGKANVEVRPKFVNKGEIVKRLVWHHHGRRQDMLVANREHLAKEEMPDFIFCLGDDFTDEDMFAQLNKIEAVWKKEFAGQSNQWNGFGFYPCTVGSASKKTVAKAHLTDPQQVLDTLGLLVGDVSLFQSAGTVDLDSRGHVKNSDSSLRSEKASAQYLMRRQHSDLSAGSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T915</accession>
    <name>A0A8J2T915_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ZYBA0S07-01948g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_01948g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat RAPTOR family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10262360at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030307">
        <property type="term" value="P:positive regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004083">
        <property type="entry name" value="Raptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029347">
        <property type="entry name" value="Raptor_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848:SF16">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14538">
        <property type="entry name" value="Raptor_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01547">
        <property type="entry name" value="YEAST176DUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM01302">
        <property type="entry name" value="Raptor_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Raptor N-terminal CASPase-like" evidence="5">
        <location>
            <begin position="79"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1020"/>
            <end position="1049"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF90477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1529" mass="173756" checksum="2CD64B7C20D4937F" modified="2022-05-25" version="1">MPVIYGPQPLRPLNVETRHGFDEQYHSEQFLQTLANDFIFYYDDKRHKTNGNPTPEEDKIEDAEHYYQPIHDWKIMKDRQKTVSAALLLCLNLGVDPPDVIKTQPCARVESWVDPLNFQDSKKAIEQIGKNLQAQYETLSLRTRYKQSLDPCVGDVKRFCNSLRRTSKDDRILFHYNGHGVPQPTPSGEIWVFNRGYTQYIPVSLYDLQTWLGAPCIFVYDCNNAGNIVSNFKKFVQKRIREEQDGIQNTSAPSPSFTNAECFQLASCRSDELLLMSPELPADLFTCCLTCPIEISVRVFLMQTPLKDSKYKILFENPTNNDFEDDKNSYKSKIPNVIIPGVLSDRRTPLGELNWIFTAITDTIAWTSLPRPLFKKLFRHDLMVAALFRNFLLAKRIMPWYNSHPISDPELPDSIAHHPMWQSWDLALDEVLSKLVNDLKNSPPASDLGSQFILQQQESLQNQAPQRQDNKSNSIQGNSRFAVANLSTMSLANHPAVQGASKRSQASLSQQPQQQQQQQFTGFFEQNLTAFELWLKYASNTRHPPEQLPIVLQVLLSQVHRIRALVLLSRFLDLGPWAVYLSLSIGIFPYVLKLLQSPAPELKPILVFIWSRVMSIDYKNTQAELIKEKGFMYFISVLVPDWGLLPSPASGTSIAPGSALSMTASPNMNGTRGQHYQSHNGLYSSAHYHSNDTTDEQKAMAVFVLSSFVRDFPLGQKYCFSLDLVNKLSFYIDNSDIPLLRQWCIILIGQLCTHNSLYRHICMNAGIIDTLLRSLKDPVPEVRTAVVLALKNFISDVDDSDQLFKLQQEFEQQYQQLHAQMQQLQSSSHQQQSHHIEQQQMKIEQQIQHCQIMQLQMQNIEPKKLKKQEITNLVGVLGLINDGSPLVRKEVIIFLSHVVFRYINFFIVVAFNELIEEIIQMERTDTEAQSYTDKHAVSHGSVFSTIWEALLILAEDPYRENRTLAGQVVDYVLLELSTHKDLSYAFSKIEHYLIKRSVGLGQGRKSPSNTNSQVQLLKKPNLPFSNDRHDSSPEGNGQEPKDKSNRSKGISLGKFLQTLGLGNSKNRSESNLRGLMNEHDMKPTNTSFLSAPHGMAPLAKIPRSRKLTGPLTLPLSSTFLDYSREYFQEPQMRKHETDEPGSIEYSARLWRRNRNENIIQQTQGEKKLALYGDWSNRLTTFDNKTQPKLIKFAQFESFLVSADERDEVTVFDWENNTHLARFSNGNPFGTKVTDLKFLNEDDAALLLTGSSDGIIKIYKNIHSCDELELISAWRGLTDMLLTPRSSGLLTEWQQIRGSLLTTGDVKIIRVWDAHTETVEVDIPAKTSSLITSITSDQLAGNIVVAGFADGALRVYDRRIDPRDSMVRLWRPGTGTRRTWINNVHMQRGGYRELVSGSTNGVVELWDVRMDEPVGMFRDENLQQQNGQKRLTTMTTTQVHEHAPIIATGTKQVKIWTTSGDLLSNFKNHHSSGGVAGTLAATGIRSTLSSSNAFLSSMAFHPHRMILAAANSHDTSINVYKCENRNTEFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9B5</accession>
    <name>A0A8J2T9B5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ZYBA0S07-05952g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_05952g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T9B5"/>
    <dbReference type="OrthoDB" id="7451790at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061499">
        <property type="term" value="C:outer plaque of mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902412">
        <property type="term" value="P:regulation of mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031028">
        <property type="term" value="P:septation initiation signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052574">
        <property type="entry name" value="CDIRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47566">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47566:SF1">
        <property type="entry name" value="PROTEIN NUD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="157"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="237"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="327"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="257"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="330"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="340"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="377"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="412"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF90649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="833" mass="92365" checksum="BE32B8D4BA1FD998" modified="2022-05-25" version="1">MGSPSKNLVEGLEGFHISSPGGSKEKLNVKQYLPNNDFADSKFLSFVEASQSRSYVKKLGDWSMSYGTVQQRPGESPQHANVDARSSFEDGFAMNNNNNDENAETPQWKQYMMMQAQTKAKGASKQPLKLQLDQQPSHGSKDPASELVLTASLSDISGIPTNTFKRHGRRKIENEISKETQEDDDDDDGGYEQEEENQEEREENLGHGHEEGEEDDIDPALEARGVFDNILRKQRSNYELPQPPTHKQPTSAQLPDSAASSETTSSFFGETPSSLSPAEPHNPAAMAQAQPFAKQTKKPIEGMKLITPEEMGLVFDNVNGVWYKPAPKNQDVSSSRTLDSSTNNSEFTTESTSVISKQQYQHHHNHHYPDHHRTEQPQEEDEEYDDTPMDAPEINPRFLLKSTQSHDARHGSSITPDSSRATGSGLNSVRGPRGSANAASAPRAASSANMPAPGDVTTVSQVHTSFRQSKRELVAVLTDAIPPRGRDWATEVLQLDLRRHSLGQVVGLDQMLPSLRECDLSHNKLRSLHGVPPLVTHLRCSHNKLGNCTLDGLPHLETLHFDHNALTRISSTISGCLHLREVNLSYNKIRLLDRELGASRMLRLDLSHNELEGTIDLAKLSSSEGDAWQHLEELDLSHNRITKLRNVNSLRRLRVLKLDGNPLQEILEHSATSGGNLRTLSVLDTTQKLQRIVSAPSGAFPYHKLRILRCDSHRGAAHWRALPRSLEELHLQGGDLHKLPHWDVLPGSLRRLTLRAVKGLTALPRSLAWRVPTLQELCLAGNELASLYGLVEALPVLCLVRLDLRGNPVAKGGLADIDRILRMACARLADVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9C8</accession>
    <name>A0A8J2T9C8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ZYBA0S07-01288g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_01288g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPF family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T9C8"/>
    <dbReference type="OrthoDB" id="361020at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000736">
        <property type="term" value="P:double-strand break repair via single-strand annealing, removal of nonhomologous ends"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20078">
        <property type="entry name" value="XPF_nuclease_XPF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000009">
        <property type="entry name" value="UV endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006167">
        <property type="entry name" value="XPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047520">
        <property type="entry name" value="XPF_nuclease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00596">
        <property type="entry name" value="rad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150:SF0">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="11">
        <location>
            <begin position="788"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="21"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="942"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF90450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1038" mass="119229" checksum="7E95B5BAF0C13503" modified="2022-05-25" version="1">MPRLFIDEDSEDEELQLELSRQTEEIKDSSNKASQELHNETNEAVLANAEEEDALDSKPLYPLIPNENIDEGLKPNLDDIRQVDIQLNLPLAFQQQVVENTLVTEDPLVILGRGIGIISIVVNLLFTLATPTRINGQPKRSFVIILNASPANNLRIEEELQELSWLSGCEEIDREEENAQRPFHVINAESQNSDKRHQLYIAGGIFSITSRILIVDLLSGLVHPNRITGMVVLNAEKLKSYSNESFILEIYRSKNKWGFIKAFSEAPEAFVTKFSPLMRKMKDLRLRNVTLWPRFRVEISQCLNNAVDPKINKVIEIKVSQTNSMSQIQFGLMGCLKKCIAELNRKNPELALETWTIDNVLDTTFVRSIDAVMIPNWHRISYESKQLIKDVRFLKNLLKLLVSGDAVDFYEELQLSIDANKPSISKKYSESPWLMADESQMVISYAKKRIYCDGEYHLEEMPKWEQLVSILEDISYQRSYRDVQGPTLIVCSCATTVSQLSRVLSQADKKDGFRRNMLRKLQIYKERREVSKRLVREVREKEPEIAPELNVSAAFTKQEVLTKRRRTRGAAAVAAVERLKSAGSGEDIESVINEYDANDELDSEGLLEISDSEEAPAIEGDFEEKFPVNNISQKVLTKEIWDQRLTDYQFIDCADQIIIEKFDNIIDDSYLQEIMPSNIIMYEPDLSFIRRLEIHRAIHRELPPNVYFMYYSDSVEEQGHLTSIRKEKEAFTKLIRENSMLAHHFEADEDLSHYKNLADRKVKLNRLRKVTTRVAGGQAALADFTQDVVIVDTREFNAALPGLLYRYGVRVVPCMLTVGDYIITPDICIERKSIADLIGSIQNHRLVAQCKKMMRNYKYPTLLIEFEEGQSFSLEPFSERRSYRSKELSTVHPISNKLSQDEIQMELATLAMKFPGLKVIWSSSPLQTVNVILELKIGRDQPDPSQSITVGKKSKQKKYEKNEAASPIDQQDYSSILRIPGISKVDYFNIRKKVKSFQRLKAMSLQELSDLFGDEDLVEKLHRFLLAEREDEEDSNIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9E7</accession>
    <name>A0A8J2T9E7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S08-04918g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_04918g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316461">
        <property type="protein sequence ID" value="CDF90862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="8436363at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052410">
        <property type="entry name" value="DRC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24107:SF2">
        <property type="entry name" value="NLR FAMILY CARD DOMAIN CONTAINING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24107">
        <property type="entry name" value="YNEIN REGULATORY COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="29"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="218"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="281"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="486"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1201"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1275"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="105"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="218"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="336"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="376"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="398"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="491"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="514"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1201"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1275"/>
            <end position="1286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1296"/>
            <end position="1318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF90862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1351" mass="149687" checksum="0430D8776EC02778" modified="2022-05-25" version="1">MPDSSHDPQKLLRAHKIGTLDVDWLVNARNLNTDDPVPPFEKKERKERAPSVQHETHNELPPEDPRSSMHIHANLPEHVSRTPARGRSNSVVNLKNPFDTDDDTSNALRKTKSLSAASPESQTRNYQRRASISVNSVPQKREKKMGFFKSLFSGIRKNSSPPPPPPPKTRMQNVTAPSSRRGSLSAGVPLTRARTASMSMGHHSNHQQEENHIFFHHHDQENDGEHPPKEDGGGPLFRARTHVTEEDRNARDSRLAEFLRYYKANGYPVSAFRHVDEATKNLDVGDKPTGPGAAFQVGGDDSGDENPPVTSTTKYDARGRPIPPHPTKSKLPPALKDARESLSRKSETQTEANSESDSEQSSSNRFGSFLKRVTSHHASTGASVSPTRSVHHHHHHHFDPTKLKDVPGLENMKPLRHVSFASNTYFNDPPQQICSKHPRRGEVEVKPNGSVVVHRLTPEQRKRILETTSAGVVVGGSGQLKLLNQQGEVGGDARKEEEKAPAKLSATQMHPDRYDEEEEEDDETHTAQHRNIRLAAAEAAAEARAKDTPNELTRVSTNNEEEVGVSGMAKRVTIDKPMISRRSIASNSSLSLSSMSSAESSDDELLPPPHAKIPHDVVYTRCCHLREILPIPATLKQLIPGSTDPIPLLQLRNPRPSLVEVWSFSDFLSIAPVLCLSLDGVTLSVEMFRIILSAILGKKGFEKLSLRNTPIDHEGWQVLCYFVSKSESLSALDLTMVPQIKTNAQKPSKSSLRSTIVRMESDMENRSDMNWDLLAASVAAKGGLEEVVISGARMPADQFENFIDVACIATVRLGLAYNALTKQQCQALSRWLVQSKVTGLDMGFNDLKGKLSALNDVIWDKVYNKRQKNVFRYISLNCTGLEVDAGDVSETNEVLKLISLLCYCEDLKFLDISNNPKIFPHCLSTLIDCLPVCSGLTRLHLDYESLEPTSVVTLAEALPLCTRLNYLSMLGNKFDLASYKALAEAVKKSPSLITLDVDYSGMPQKIREKLSLYTMKNVDNELNRVRSKSNTSGEKSPDSELQEELSRLLSGDIENKELYDELVQKYVQRVNIVRTKIGKVVHDLFAIRMQGQLNVEGKETLIRLCFIDASLEKRLELLEERMGKKTDTNPFSVSNSLSHVSTNRSAASEATIVPSSMASTAGLEHTGHSALLPFGRAEVEAGQEQVPQADDTVELVDKDENGNPIKHDDGPVYGVSHHHRHSSADGVDKEQLGKAAKSLDTDKIKDYLMKSDVNEVVDVIDELHNQGYRLHDLFKKQPKSSAERARLPSKRVPLRSRTSSTSVNSLSSSTSKAGSLSSRPSTDSLRAVRAENEAIDAAYDQVLDNLQRDRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9L1</accession>
    <name>A0A8J2T9L1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ZYBA0S09-02564g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_02564g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the importin beta family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316462">
        <property type="protein sequence ID" value="CDF91000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T9L1"/>
    <dbReference type="OrthoDB" id="361693at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058669">
        <property type="entry name" value="TPR_IPO7/11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF7">
        <property type="entry name" value="IMPORTIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25758">
        <property type="entry name" value="TPR_IPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="5">
        <location>
            <begin position="33"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF91000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1029" mass="119010" checksum="9FE9A863C708C662" modified="2022-05-25" version="1">MSETAVLNELTLVETLEQASNPQHAGSEVQKSAEKQLEAWQTQPGFHYTLQSVYLDLSNSLQIRWLSVIQFKNGVEKYWRSTRLNAISKNEKISIRRRLFDLIDEQNNQLCIQNAQAAARIARFDFPVEWPNLFEQLEQLFGDERIMSSAIKIYNVLMHVNQIIKILGSARIGRCKPAMQSKVPLIFPMIVSIYLRSFNTWTESLGTDEDNSSSQQVSYLALKVLRRTIADGYERPQKDESVCEFMRMTVTHFDLLISHQENFRKSDVYDKFVRCYGKLYYNIITNSPANFILLPCSTQILITYTRLLFERAPDVYNENPDTGDFWEQTAIRGFLILKRVVNFIHKRGAVVLKARSDRFSVDAAMQRISNEFLNEKLVTRLLDTLITWYLKLRPSELESWFMDPEEWMNEQLSSSYEYQIRACAENFFQDMINSFSELLVPYLLKKIENDAANLPNSLEGFLSKDAIYASFQLSAAAIGEMVDFDRLLVQVFLPEATSSSEPENEARVIRRRVCLIIDEWSTVKCSDDSKKLCYEYFMELLAKENDAVVLLTCVQALRTMIDDWNFDKKSFEPYLNGVVTLLLRKILPAVALTETRLYILNTMSDIIIQTKPLISRDLLIEILQIVPDLWQVSTNSVSEYILSNGLLRLIKNLVTSLGPHSHLTWTISLPIVAQSCNPSSPIYQLVSEDGYELWGALLQNFSPAEANFDPEFIQLIPFLEFGIDSHTEILPTLLEIVRSYSLILNLQEFFSCPTFSKIFSQLSRYFLKLRDDAFELTLNIWENLTLFNPTDYEKPLLEYFHQDGVLQAIFDSIFKEEPLSSYQCAQVIQICARVAYVNPQALIDFISAYHQHLPSFTQNQELSISERRVVSSSMSVEDVLTKFLSVWIVCFKDIFDPKMKKVHILGISSLLRTGMYPVLSEFQIIVSMWVDMLEEINEANNGDCEKYHLNDIITEHSIEYPPTSEQIRYHELCKNNDPAHNVSLKSFIRQILEFLENRMGTNYQELLNSVNSRLMESLQLFLEISFPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9R4</accession>
    <name>A0A8J2T9R4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoinositide 5-phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_03048g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptojanin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.36" evidence="4"/>
    <dbReference type="EMBL" id="HG316465">
        <property type="protein sequence ID" value="CDF91617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T9R4"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000029">
        <property type="entry name" value="Inositol polyphosphate 5-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF269">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE 5-PHOSPHATASE INP51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SAC" evidence="10">
        <location>
            <begin position="147"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF91617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="934" mass="106364" checksum="614924425DB75220" modified="2022-05-25" version="1">MRIFVGKSPRSIVLCSNGYNLSFRRLLGPQQQQQYLKTPIVALKSIPDRELTEENGYLEVKNRILNGLLGLVAVNGDLFVAVITGVQKVGFPRWRQEDKRIVTTENVYKVLDVDFHSLDTSVFDYLFQEISEQNYEKLVNEHPCGPLKKLFSDGTFYFSRNFDISNVLKSHGLAHSMDYTIDNQDKNFIWNANLVTEIINWRSRIPAEEKDYFDGEFLTFVMRGFYKTQVVDMTDTLATITLVSRISAESKQNAFDLDGIDEDGRVPNFIETEIIVTTNDFIFSYTQINGNVPLFWESSDGQLLYGRKLKLTKSLEHTQAAFDRHFDNIASKYGVVSIVNLIKPRSESQESLANGYKTCADSKGIKITNVDYGSGLLTKTPHKLLYLLKQDIYEFGAVVYNRNKGVYIGKQTGVLRISAFDSIERPNTVERLVSREVLELAAGELSGLDITSAFLNVHDRLWSENYYWLERTYAKNVRSPGKYKKVYAKLFSSKIKLYDPLHSTVAQYLRQRKATYTFAKNITIFVGTFNLNGKVPTEDLNSWIFPDPSKQEDMYVIGLEEVVELSPGHMLTTDPYLKQHWEKIILGQLNSVGKKKYGCPWSSQLGAIMLMLFVSEEEYPKIKHIEGDVKKTGFGGMASNKGAVAVSFKYSATRFCFLVSHLAAGLENVEQRHHDYKTIVKNIRFPPGLRIKDHDAVIWMGDFNYRILMANDDVRRLVSEKKYANLFEKDQLNQQMIAGEVFPYYNEMEINFAPTYKFDPGTQTYDTSEKMRIPAWTDRILSRGEVLKQLTYGCAEDIIFSDHRPVYATFKAQATVVDEQKKAALSHNIYGEIAEKLATMSEEEKIAILNGGGALIDGIDQESVTSLPENTPTGAKQKRALKPPSSELSRWWLGNGKQVKVVLDVDPQQYMINPKKAPNPFINEDSDEPLFVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAF5</accession>
    <name>A0A8J2TAF5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycerophosphodiester phosphodiesterase GDE1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_03862g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316463">
        <property type="protein sequence ID" value="CDF91284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="197419at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047389">
        <property type="term" value="F:glycerophosphocholine phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08606">
        <property type="entry name" value="GDPD_YPL110cp_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14484">
        <property type="entry name" value="SPX_GDE1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057506">
        <property type="entry name" value="C2_GPCPD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051578">
        <property type="entry name" value="GDPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22958:SF1">
        <property type="entry name" value="GLYCEROPHOSPHOCHOLINE PHOSPHODIESTERASE GPCPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22958">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25329">
        <property type="entry name" value="C2_GDE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SPX" evidence="6">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="572"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="606"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="7">
        <location>
            <begin position="979"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="666"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="864"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="684"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="706"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="892"/>
            <end position="902"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF91284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1318" mass="146101" checksum="D3E434C53419D717" modified="2022-05-25" version="1">MKFGKTFPNHQVPQWSHQYVNYKGLKKLIKEITQEQEKLYRQDHEHLKTRNSPPGKVRDSSKVEENYMDNEGVKKLLAFFFFALDRDIEKVDNFYNMQFSEYERRLRRLTSSAQFAEINNSLMTQSGISLVPQGMHHPRPEINGLNPNYVDQMQSSQSMMPTMSQTPEDISEDLAEVLGILVELRSHFRNLKWYGELNKRAFTKILKKLDKKVGTNQQHIYLQARILPLGFSNDSEIVRCLNVINDVLDKVSFKVQDKLASGKKVAEKEIGGVELKNGGPPNDIILYLIEKNDGIALINELTAMYRSLVLIPTRILVNLLNKAALSQSFQCMDELLKIIPTLRDPSDINARNFFHHHVIALGKNHKKLREDTAYASVKANGALQGSVRNEENLDNMTNLNSLLKETLDLEAAELPDHNSRLIGAFGPDGINSNDSPASLVHILDKLPAHLRPSLLQRDNYKRTPLHYSAQYGLVEVTRIIVENLKKWGAWNADISIDNTSIWGDSENLTPLHLAVIGSHPQTVKTLLSYVNHAQPVTCPQLLHLATRLNSGALVEALLSAKGFDLNFPDEDTCETPLYLACKLNLYEAATCLLEHGADTELREKLFGWTPIFVAATEGYERIVQLLLDHGTEYVVFDESGWTPMEHAALRGHLKITAMLKITGHPGISHPKIPSDEVEAVTRGSPPSESVSSPASRGDSTSSSISGHNNENLKGENQSSIEHKPKNGHVKINHRELLRSSTARSNSHHKHHAPLPQPVKSFGHRYLDHDESLLMITLGGNDSRNTKPAVSLNKVPVSKVSSTQLDTALSLVITCIDDLSETPFVLDLPLDDNLDAVTFKVPSKANESHVVLFDIVPTYGSVPGRPSESPLCSGNSHAHPYPADPPMVGSGGSLSSNNTNGGNTAPRVVGRAVALLSKTGAFVGVNRRSLNDVITVPIVANGTMDVLGTVSFEHVMVTAFDHPKMSVGRTETYWKSLVSTRVIGHRGLGKNFSSKKSLQLGENTVESFIAAASLGASYVEFDVQLTKDNIPVVYHDFLVAETGVDIPMHELTLEQFLDLNNVEGHMDVVNRKDRRRSVDDTETSIYKTSFDDSLAKSGAHEDVSKLLENRMRLTRTYKKNNFKGNFRGHSIASSFVTLKELFKKIPQNVGFNIECKYPMADEAEQEDIGQIAVEMNHWVDTVLKVVYDNANGRDIIFSSFHPDVCVMLSLKQPSIPILFLTEAGTTQMADVRASSLQNAVRFARSWNLLGIVSAAAPIIQAPRLAQVVKSSGLVCVTYGVENNDPENASIEMDAGVDAVIVDSVLAIRKELTKANVNVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAH9</accession>
    <name>A0A8J2TAH9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="15">ZYBA0S11-01354g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_01354g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316464">
        <property type="protein sequence ID" value="CDF91371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2TAH9"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306:SF6">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="14">
        <location>
            <begin position="77"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="256"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="774"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF91371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1110" mass="127397" checksum="BA9FA1D96BF18571" modified="2022-05-25" version="1">MLPTLSSNKRNFETFDNDLISFANTNESHANETRRIQLKRRHYNVSPMTQFPDTADASTQETQSSLMESSPSGHIKKITLWNFMCHEHFELELGPRLNFIVGNNGSGKSAILTAITVGLGAKASDTNRGTSLKDLIREGCHSTKIILRLDNSSYGAYNQGKFGKEIIIERTIRKDGTSSFSLKSENGKEVSSKKRDIQAAVDFFSVPISNPMCFLSQDAARSFLTASTSAEKYRFFMKGTLLQDVTDHLEQAKSISSSAQESMSLHQDNLNNLKEEYEDAKKLLRELNQTTDLNQRKRLLQGKALWLDVAQNKEACRLLREEIAAYEKQIELKLAKMRSKQEKIDRICVDRSALEKEVENKVLSVAEKDSEHQEARDLLRNIKANFEKERQNRVEAQTSIDQCAQKIKTLNSYITRLEEEIRSEMGGDKSQMRKHIEQLENENEKLSKVVSSLSLELQDLQNEEKRITQERNSEIQKMNHELQRKKNELRQVSQGNSNFLNNFDERMILLKQTIEKRAGEFESPPIGPIGSLTTIKGDFGKWTRSIQRAISSTLSSFVVSNHKDNRLFRELLKTCKIKANIPVITYKPSHFDYSSGKSCCGYPTVADALEFSSPEIECLLVDQNKIEKIVLVEDKEEARKLLKQQPRNVSMTLSLRDHRSGYQITGGYRLDTVNYQDVIRIKVGSSSDEGIEYVKDLINNEIREIDMVSYRYGQKLEEVHAKILTIDKELRQTRRNLKDNNINITNLKINVGKEVDTGILGSKLEEKKIQEQAINGYKTALEELDLKIEQIAQRVQPLKEEYNNTRSDLLKNQEELQELKEDINNRASKVAKFNDDIRHYELKIDSYKEKIEEKKNNIRVLERGIEDQMNNAQHFCSEEQIECADIPSNPEEIKRELENISKLMRKAEKQLGLPQQKVLEIFEKSRDKYQEGQEKILQMSNALDTLYNSIQVRLQNLQSAQRTTCLDADLDFRASLRVRNFSGNLSFITSSKRLEIYILTPNDEKARNVDTLSGGEKSFSQMALLLATWKPMRSRIIALDEFDVFMDQVNRKIGTGLILKKLKDIARTQTIIITPQDIGKIADINSQGVNVHRMKDPERQNNSNFYEHGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAI1</accession>
    <name>A0A8J2TAI1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ZYBA0S20-00276g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_00276g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316473">
        <property type="protein sequence ID" value="CDF92062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001262">
        <property type="entry name" value="ABC transporter CDR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005285">
        <property type="entry name" value="Drug-R_PDR/CDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00956">
        <property type="entry name" value="3a01205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="491"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="524"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="575"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="602"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="633"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="739"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1173"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1244"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1294"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1323"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1353"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="121"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="837"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="430"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="793"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="437"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="794"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF92062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1498" mass="169939" checksum="94149D95ABD1FE91" modified="2022-05-25" version="1">MTESNSETPLYQGFEQEAQEYVKGLAKTFTRQTIGEDNNEDHSLQPVNPIFQHGDGIYDEKLDPNSDQFDSKAWVKNMSLIMNGNPEYYIPYSIGCCWRNLSAHGASSDVAYQLNVANVPIQILQWVYRHIRPKREGDSVQILKPMDGILKPSELLIVLGRPGSGCTTLLKTLCSNTHGFALSKDSEISYDGLSPKEIKRHYRGEVVYNAEADIHLPHLTVWQTLITVARLKTPSNRIKGVSRETWARHVTDVAMATYGLSHTRNTRVGNDLVRGVSGGERKRVSIAEVMICGSKFQCWDNATRGLDAATALEFVRALKTQATLLNCASAVAIYQCSQEAFDLFDKVCVLYEGYQVYFGPTQEGKKYFQDMGYYCPPRQTAADFLTAVTSPAERIIRDDYKESGKHVPQTAEEMFNYWKNSSNYKKLSEEVDSELSSNKEPDRENIREAHIAKQSKRTRSSSPFTVSYFQQVRYLLIRDWWRLINSFDITFFQMFGNGTMAFILGSMFYKVMKKDSTATFYSRGASMFFAVLFNSFTSMLEIFSLFEARPITEKHRSYSLYHPSADAFASALAEVPPRIVTTVIFNIVFYFLVHFHRNGGRFFFYYMMSLIASFAMSHLFRTVGSVVNTLPEAMMPAALILLALSMYTGFAIPETKMLGWSKWVWYINPLSYIFQSLMINEFTGRKFPCARYIPSGPGYENVKNTEKVCAAVGALPGKDYVLGDDFLKLSYNYTNIHKWRGFGVGMAYAIFFFFVYLFLVEFNEGAKQKGEILVFPHSVIRKLRKKNLLNKTGNTDEEKCSSSESIKVSDKKMLEDSDDHSSNEVSEVKLSKSEAIFHWRDLRYDVQVKKETRRILNGVDGWVKPGTLTALMGASGAGKTTLLDCLAERVTMGVITGDVFVNGKPRDASFPRSIGYCQQQDLHLTTATVRESLKFSAYLRQPADVSKREKDDYVEQVIDILEMGAYADAVVGVAGEGLNVEQRKRLTIGVELVAKPKLLLFLDEPTSGLDSQTAWTICQLMKKLAKHGQAILCTIHQPSAILIQEFDRLLFMQRGGKTVYFGDLGKGCKTMIDYFERNGSHQCPPDANPADWMLEVVGAAPGSHANQDYHEVWKNSAEYAAVLEELDRMEKELPSLNEGPDAGSEKAFAAGTLSQCLLVTQRLVEQYWRTPSYLWAKFFLTVINQLFIGFTFFKADLSMQGLQNQMLSVFMFSVIFNPLLQQYLPTFVKQRDLYEARERPSRTYSWVAFMVAQIIVEVPWSFLAGTLAFFIYYYPVGFYSNASMAGQLHERGALFWLFCTAFYVYIASMAVCAISFMEVEANAANMALLLFTMGLSFCGVMATPKAMPHFWIFMYRVSPLTYFISGMLAAGVANVPVKCSSYEYVAFVPPSGKTCGEYMSEYIKYMGTGYLADSSATSRCEFCQYSKTNDFLASINTYYKDRWRNYGIFXXXXXEYPKRVAQKRNNNTLLHRTIPSGRTISRSRLYLYNTIEYYKGCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TB14</accession>
    <name>A0A8J2TB14_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S10-01508g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_01508g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316463">
        <property type="protein sequence ID" value="CDF91181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2TB14"/>
    <dbReference type="OrthoDB" id="1204at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010513">
        <property type="term" value="P:positive regulation of phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903778">
        <property type="term" value="P:protein localization to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024260">
        <property type="entry name" value="Vac7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28258">
        <property type="entry name" value="VACUOLAR SEGREGATION PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28258:SF1">
        <property type="entry name" value="VACUOLAR SEGREGATION PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12751">
        <property type="entry name" value="Vac7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="695"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="402"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="613"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="500"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="622"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="634"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF91181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="898" mass="95441" checksum="52E6592D42CB08F5" modified="2022-05-25" version="1">MSDEDHKLTVETETVEAPGASLLPSTGNGGPNENGPETGNNEQDSVNVVTSKDISPVQFASSYAAAAHAAAAAAAAQAPASSEASVNLINHNPLSPHAIQSLKNKKSSLLINNERNKETLGARGGEDEQGGSAASGGLGNSANHGNGVVNPGASGVGGGGGGPSEAAGSAQGDQHISQMSHSSKAATAPPGQTYSQQPKPQDPKLAKMIQRPSLPLMNSREILLESEKMNSGTEKEVPGSSNPEPTAAPPQEDDSESLHKPTKADFFAARLASAVGENEISDSEETFIYESAANSTKNAVAGGTTEALPATGLGSQQQAPLTQVPPSPHILASGAQLPQQQQQLQMQVGAGPGAALPQIPLSPQVPHGVASKMSVPVLNNNSKLLNRLKSTRHTSLSALPANSSSTFATVPPAASPITNTMPVQPSTEDLSSLKSFARRPGTMDLQSVKSFSSEPRSPDKRLSLVSLAKGGNARKSSVAAAVPPPPPPPPPPPPFGAGATTHTLSRKPSISNSTLRHVSVSRSKPDPPSFKRKLRTTASKIFDPNGARRYSGIPDDVNLEDYIEQQQPLEPHRIGHELADDQQSTIQEEDAEYGGDVDEDDIHSMFFYNHRGNLESRPPISDYDEDDDPPDLENNTNNNNTTANNNNSNSNPLPYTATEYTPLKPRRQLSHARLGYSPHNFYTKKASWARLRHCLYFSFVTTTLLAVGFILGFLLASNKELQDVNMVLIDNVLSSVDELVFDVTVSALNPGIFTVDLQEIDLDIFAKSAYAEQESVYSNDDGNRNQGHSTQTVLLGTAYSLETPLRFRGNFLRHNYDVSVSSVKLLDPGAKQDSVATAAVLQPPALQLQQLEDDVAKWKNIIKHDYVLIVRGSMKYSVLLGGRKSVPVQCQADVKVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TBV0</accession>
    <name>A0A8J2TBV0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ZYBA0S15-01530g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_01530g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316468">
        <property type="protein sequence ID" value="CDF91917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2TBV0"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015489">
        <property type="term" value="F:putrescine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11476">
        <property type="entry name" value="SLC5sbd_DUR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031155">
        <property type="entry name" value="DUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154:SF4">
        <property type="entry name" value="UREA ACTIVE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="422"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="450"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="490"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="558"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="591"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF91917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="669" mass="71746" checksum="B7749BEFE2C1035A" modified="2022-05-25" version="1">MEPILSQGYGYGFAVGLGAAFALLMAVISRLLSKYAGQVQNSERFSTASRNVSSGLIASSTVSAWTWPATLLTSGTWSYVHGVSGGWLYGVGGTVQVTLFVFLALKIKQHAPTAHTIPETFYIRFGKAGHLLFVFYCAATNIIVSSLLLLGGSQAFSAATGMHTVAASFLLPLNVIVYTALGGLKATFISDWVHTVIIYVILIVSAYTIFCSSSLIGSPGKMWDMLKEVQEVFPASGTSFLSFKDKDMILITWSVMLGGLSSVFGDPGYSQRAIAADSSSVFAGYMSGSICWWVIPFALGSSAGLACRALLTNTASVTYPHALSDAEVNASLPVIYGLAAIFGKSGAAAGLVMIFMSVTSATSAELIAFSSITTYDIYRTYIKPNATGRNLVRVSHLAVVSFGIFMAALAVVFNYIGVTVGWLLSFLGIILNPEVSAITISLFWSRMTKLSLLIGAPLGTVTGVVCWVGATHRYADGIINKTTLMTPEATFIGNITSLFSSAVYIVIISFIRPEPAFDMNEWSEEIKAADDIDAEELSALKVSDKGKRLLSKQANWSLLINIFILLGVYVVLTCALYGWGGDMSKGSFTAFMVIMLIWLMVAAIYIILFPLWQGKQAIKMVICNALGKGEIPDQGKFSDQVENEGSDSEVIVEQASSKKEAEITILEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TCA2</accession>
    <name>A0A8J2TCA2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_00584g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="HG316468">
        <property type="protein sequence ID" value="CDF91876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="162894at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901992">
        <property type="term" value="P:positive regulation of mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05611">
        <property type="entry name" value="STKc_Rim15_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000340">
        <property type="entry name" value="Serine threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001008">
        <property type="entry name" value="Serine/threonine-protein kinase cek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GREATWALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="696"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="1097"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="13">
        <location>
            <begin position="1439"/>
            <end position="1553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="450"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="515"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="858"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1254"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1327"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="454"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="520"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="534"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="600"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="858"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="894"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1268"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1327"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1351"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1374"/>
            <end position="1397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF91876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1569" mass="173698" checksum="1A6B2F0C9562CC87" modified="2022-05-25" version="1">MSQLSEDESHVTPKRSKSITSLDSESSSNYDKYLQLATEKNPVMLLELDQDGTIRYLSEMWETIVGPREYGQIADLIIGTQEDKHVFHRAMEMMLVNDSVSYTVTFNVMAKEADVGDGEYQCDTARLITLESCGILIRDSETQLPTHSMWTVKPYDPEWYGEETLADLPAEFVKRLGFGATIFAEYLRLVDYEQCLDSVDLPPPKMELCRVCETFVPAWWLETHAQSCVCEHRIDSYIHLLHDNLVEQAALFQNNPELDKGYKGLPVNINSSSSRSIVDSLKELCEISININPSELRSQKRATTEDLLLNPICLTQDNLEGIYDFSPRSKWNIQNVKSWQLSFKEELDQDPGLALLVRDTIDLAKQKVEALLRLDNAMTYSLRIKNEVNTWVLQLISQHIETNKINLRLFLGYTDSPEPQESSEAHLGKVGSSSAGQEALIPSTALAEGGIVHSSSSSSKPSRNISRSHSRSASQARSRSASSSFAISPKIASPQPQRPRSELFANSYLIADDLPQGDTENVKKENDILRRDNTSGSNVSTTNRTSKSHSRSLTPRQRFDDQHSVFLRNSSGSPLDEAGERTQSRPGSVPSFGALKGDSKNSLTVSKLKSSFSLTPGRSSPLPAALAGGPTSRKSSGSQPRGGADSSGVSSPFANARDYLTPEQHPSVSNLPKQPLSPLLLATNQVKSPAPSIKDYTILKPISKGAYGSVYLARKKLTGDYFAIKALKKSDMISKNQVTNVKSERAIMMVQSDKPYVAPLYASFQNRDNLFLVMEYLPGGDLATLIKMMGYLPEKWTKQYISEVIVGIDDMHQNGIIHHDLKPENLLIDSKGHLKLTDFGLSRAGLVRRHKNISQSKQFSFSNASANQSPISPLMSSNRNHHKRKSSLKHEFSESPSMLSPSTDEVSNRGRDNSESSSQSHGDILALKRSDSQVSFSILNISRSSTPTPPLFDALHSRTNSNTSDKMIDTNSAMDLALFHPEDSKQDKMFFGTPDYLAPETIEGTGEDDACDWWSVGCILFEMLLGYPPFHASTPEEVFKNILAGKIQWPEFSSLVDQKEFESSSSRDLISKLLVLDPTERLGYNGAREIKDHPYFRDVDWDRVYEEEPSFVPAVDNLESTDYFDLRGAVLEDFGPDEDAHDVPMNSENDMTYSPLFQPNDASKRSSGTIANSNDNIVHPQVNKLNITANELESHEGSNLSSPTLKHIPLAIPPHMRDRRVSKLNETQTEFGSFYFRNLSALDKANKDAINRLKNEHSDSPGIHRRTSSASHLSSSSEGSGSKIKAGKVSVSGSPGGNLIGRTSITRAASPCFRASSPERIMSIDISGGLSRKGSGESSSNSSNLYHYQFNDDSPSSAKLKSPISPNGANNALSTLISSSSSASSRTRTTVKSTSQRANSNENFENDRLQAISKVQSMRYRRRSGRKSLGDDDFRYHMDVLVCEPIPIHRYRVSRELEDLGCAVVSVGAGDELISRATSGIKFDLIMTALKLPKLGAADIVTLLKHTSGVNSTTPVVALTNYYHEAVSVNLFNDVLERPVLFDSLKKLIAKYALRKSQQQEETIFSDND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WV92</accession>
    <name>A0A8J2WV92_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_13784g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_13784g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WV92"/>
    <dbReference type="OrthoDB" id="69928at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005697">
        <property type="term" value="C:telomerase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018834">
        <property type="entry name" value="DNA/RNA-bd_Est1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019458">
        <property type="entry name" value="Est1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045153">
        <property type="entry name" value="Est1/Ebs1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696:SF37">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY FACTOR EBS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696">
        <property type="entry name" value="SMG-7 SUPPRESSOR WITH MORPHOLOGICAL EFFECT ON GENITALIA PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10374">
        <property type="entry name" value="EST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10373">
        <property type="entry name" value="EST1_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Telomerase activating protein Est1-like N-terminal" evidence="2">
        <location>
            <begin position="80"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/RNA-binding" evidence="1">
        <location>
            <begin position="217"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="798" mass="90667" checksum="D866B1E708A39D84" modified="2022-05-25" version="1">MTTLPSGIEKNVSDTISAFSEQLNATFKSNKIVQDYALLNGFLSFVHSKLNRLIMEILKQQQDIYEGKEGEIDDKSIPLILRLYWEKIAYPIFKWFQMWRKMLLPKKSKEQPKYVEFRRMNTKLTKFFKSVHNFYYGVLETFLKEYDLSRVIPSELLQELSKEASQEGVKLDPSNRFTVLVVVTSNSCLLYLGAAQRYKSLSEKLSNKYSTADFKKAFRYFDLASLILPSIGEAHLQKGMIYVQTDNLGCAIYEFTRGALSRIPSPAGLTNFANIVCEKDSNLRERFDAVLKETHLQEMEGTKIINREIIEFYFLALFGAHFAPQSWLNENDKKCLLEGVGIDHLKKVMYEKISTRYLKNIETIFNSLITVIGGFDLLLILRIRSNKPAFDVKSVNLKELDNTSITYLEFAFSFITHIMNDVIRDCWQKNLTSFQYLAMVRIIECWIKSNRAVLQYSHRDEEFCKSLAQLLNNIGQSGIVEVSPSSNHRPRRTYFFQEDVDLKEFSCIKYALTDFNDERIFSMEDAPYRLLNRPPEQDKLSPEEEGKLRLDAIVCSGVKFLSKNSCGIGWNALNKVYESHPKPKKIEVNNCNMSNPVSLQPVRGPTAKIAAVPGVDQRSLDYSKQEYVNEISKWGYSGSSAPMAPSSLSTKPSSSLTNTVLAQTLSMERAEYNNTYAPQSTVSSTASSVVSSAPTPNVPLVAPAYPYQMYPGAMPAATAPQPQHPFYPQYPFISAPPPPPPPPSQEGFQQIQAPVPYMQRQEAAFFGNPSLGQQSMYYGQRAFPYGPQNGNNNGYSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WZB8</accession>
    <name>A0A8J2WZB8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S04-01926g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_01926g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WZB8"/>
    <dbReference type="OrthoDB" id="4036548at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="809"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="676"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="851"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="911"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="990"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="270"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="874"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="915"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="948"/>
            <end position="962"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1018" mass="108142" checksum="F3B1CC77771CD095" modified="2022-05-25" version="1">MEVNFTSIWQNLSPSELLTSASTHWGGTSTTVEPVSSLLASASTSLPLDSARWQSTSTSTYVYSTTSAQTQATNEYTSETLTSSPSYDENNFYSSSRPQSTYDYSNSNILRSTLTSAPSSTTAYETMSYSSAGSQADFYSDHSDLSIVTTSTLSYPTSSSSLIPSDYYESATTSTSNAKAIFYSSSNSPSWTETTDFEISETSSWWSYTSSPTWDDSYSPSSSNTGSASATRTTVSDTFGIQTGAYITDDSESSTSSGDIWASSGETLVPSYTSPSASPVPSTTTIEYSSSSYTSPGESSVSTLSESLIPSPTSSSTSSVLIATPGEPSIPSSSMLIATSNEPLIPSVSTPSMSPILATTSSESLIPSVAPYSTSLVYGTPPEFSSSSAIFPEASIISTETSSWDSMAAPSYYSSEYSSSVSTPLVSSISETAELSTTSSTVEWYSSGFPTSLYAMTPSISVSVDSLTLPTPLSAYYTTTEQTSQIWTSSDTFPLEATTSFAAESTGFPSLYNSATHTTPISTWEPISSTWGPSTTESFEVSNSNFYYSSIIPTDITSTVESSVNSVTNPVYWLDFTSSTTTTSIGEPTSSDYTLTTSSYIPFTTTEWSDATPTTASSVVEAALTTASIWSGSTPLDLLPSSSFTSSSSGFFTSSTESAVPSPSIRLNYTSSLASSTGSATKSKNSTRPTSTANSTTLATSSKLSSSRLSSSHSSSVTSANTGYSVSYDTKYAYYIYTQTYIITDATTTFGTGIPTTVEEPRSVSTTFSAPQTIVTQDMQFYNNWLNSGVGHTNAQHSSGSGKSDTGKIVGGVVGGVCGFLACCLVTWIFFRRRRNGHKGILKSAQGFSSEIGSRMDDSSSQEPQVPAFGEKPQASSDNNNNGTPLFSIFRTQKIPETENGEANLDLFLNPNQQRESKASENPFRDEFNFRRRPAIPPPVPPPRKMHSNTQYPATSRSSNTPRDNRSSYVSSLGDSSFVSSLQGDYSTLSSGPIRLDPGNYNAGNPEGPHGGFFREVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WZV7</accession>
    <name>A0A8J2WZV7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetylglucosaminylphosphatidylinositol deacetylase</fullName>
            <ecNumber evidence="2">3.5.1.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_07536g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIGL family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.89" evidence="2"/>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WZV7"/>
    <dbReference type="OrthoDB" id="440160at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000225">
        <property type="term" value="F:N-acetylglucosaminylphosphatidylinositol deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993:SF11">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035271142" description="N-acetylglucosaminylphosphatidylinositol deacetylase" evidence="3">
        <location>
            <begin position="19"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF89574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="281" mass="32124" checksum="F8D3D8E936329A75" modified="2022-05-25" version="1">MKITKVYFLLWLIFLGFSSRIKLRNSTVYHNELNFLNDTNSLSLIVGHPDDEVMFFAPTLLQLDKFMPSSVDFNVVCLSKGGADGLSETRVIELQRSVNFLLANTKRKVELFQFDYPDGHDERWDQTSVKNAIKSAVLENSDVQRAVLLTYDSHGVSNHPNHIACYNAVSALLHDEKNVDAALYLNSYQGNMALKYSSFFWELCKLTRQWLASHVSASPPALASVLALNPTVTLMSTYTQYILSFASMMGAHESQMVWYRYAWWTFSRFVFVNDLKVHFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X0F7</accession>
    <name>A0A8J2X0F7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Condensin complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_02190g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the CND1 (condensin subunit 1) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF89808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X0F7"/>
    <dbReference type="OrthoDB" id="436262at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010032">
        <property type="term" value="P:meiotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000272">
        <property type="entry name" value="Condensin complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000687">
        <property type="entry name" value="Condensin complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026971">
        <property type="entry name" value="CND1/NCAPD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032682">
        <property type="entry name" value="Cnd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007673">
        <property type="entry name" value="Condensin_cplx_su1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024324">
        <property type="entry name" value="Condensin_cplx_su1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222">
        <property type="entry name" value="CONDENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222:SF2">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12717">
        <property type="entry name" value="Cnd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12922">
        <property type="entry name" value="Cnd1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017127">
        <property type="entry name" value="Condensin_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 10" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="6 10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Condensin complex subunit 1 N-terminal" evidence="13">
        <location>
            <begin position="68"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Condensin complex subunit 1 C-terminal" evidence="12">
        <location>
            <begin position="983"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="872"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="872"/>
            <end position="890"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR017127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF12922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF89808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1167" mass="132257" checksum="CC1AC2ECCD6119EF" modified="2022-05-25" version="1">MVGFNLAESLTRFQSNDKRSFPQVEGPIDELNRVTDRLAVSAEQIDAEEDTLDTLIELCQGFPHLPKKLQSQLCYLVTSSMSNLAIEIKSNLGGDTNQFEVMELILQWKVHLEEYGYLTYVLLSFLQEDVHIAATQTTTLGRGGSKKTSVGGAAVELFKKTCVQLESICDAIINVLRIDLNKLFQTTAEKNMFIELFTKPLYLLIEIEPSVKVSTLKFLIQRIICMSVKYHNQESSAENAIMTNLTYFLHLSNFNAELLKTLNDVYDYPQLTEEVLRDISSRVFNSKDTTGPKAISNFLIKLSELSPHVMLRQMSLVIRLLNNSSITLRCSVVEACGNIIIGFAKDQEELDHYKQQILVLLELLEERFLDSNPFVRTKAIQGCLKICDIDSKFNKSRSKFAKLAVCSLQDRSSLVRRNAVKLLSKVLLKHPFGAMHGTQLTLSIWENYHSSAQKKLEIFCGKNRKSLDQAIEASFIREEEDVSESDVSNMENSHLYGDDLEKAHQLFKFERESETMNTVDRNTILKLKLLVVYYEDAIEFIKDIHCSLEFATNLLFSKNRNEVLETMDYFVLADAYGLEPSRNGVKKMLHLVWMKGANDEGTSISSHLVDCYQQLFLTAPEGFPFREKVTFLAKNLIQLTIGASIADIASLEQLLIMMYNRNLIDEDVINTLWGIYNSAPSDTKHPEKFTREQYHGSVIVLGMLSLANNEIALRGLDSLLSVGLGEAGDHDLVLCRYSCLALERMIPKDSGGFTPLTGEDRQNEVVRKLYSKIISYTTDLECYPMLEQALNALFTISSKPDVVATELIREKTMMTFGKPEGDESTNGPPTSRVVSLSQLLFIVGQIAIKTLIYLESCEAEFKKRKIAAEVRQSKEKDNRDENESTEKGNDSELEMIGASNEDDFADAIIFIRENELLFGEKSLLNKFCPIVEEIVSNYARFNDQILQRTATLCLEKFMCVSSKYCEKSLPLLITVMEKSSDPIIRSNAVLGLGDMAVCFNNLVDENTDYLYRRLHDDNIMVQRTCLMTVTFLILAGQVKVKGQLGQMAKCLENPDQGISDMCRLFFTELATKDNAIYNGFIDIFTNLSADQSLQKDGFKRIIKFLLSFVDKEKHQKQLSEKLLTRLPKCDTQKQWSDIAFVLNALPYKNEKITEALEKGFTMVSARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X2N4</accession>
    <name>A0A8J2X2N4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_04258g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X2N4"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17998">
        <property type="entry name" value="DEXHc_SMARCAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002378">
        <property type="entry name" value="ATP-dependent helicase FUN30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000014">
        <property type="entry name" value="SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="517"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="889"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="110"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="210"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="420"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="11"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="328"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF90576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1059" mass="121512" checksum="06644C567ECF9431" modified="2022-05-25" version="1">MNENNDEVQVPESSSPNNAKLLSSSPLKQEQASSDTSTLLRDKFSFIPGNSNGRPHFEVQSSVPSNPETQLAQLASEFPDFSPTLVQAVFKSNSFNAKLARERLNRIRSQRQNWSVSSNKKKAHSMQQSYQQQQSAMGRASPMSTNRLSSIKQNDSSSKITVERQKTSIFDRYSNVMNQKLRPTMTDSLVIDEESLAKLGIKSQEPLTANKRRRLVRADNIDNRKPTQLDKAKENHLKMKKRGGLSDGDETAEEMSGEEEGSAEEYEERTPEVNIDEQILHFLNTADASDVADLGETSMEKANIIISNRPFSSLYAFSQMELLNEAEQEKRAQDAQKKRTRRVQPKKEGERVLEKINQSIRGYNAIDSLIKKCSTYGNLIASHMKRWGIDLEHANKDEELNFMSVDSDDDAVVEDFDENAVSATPTPTPDNGLAEATKKKSKRRGSDDDYDDDEDVDFEESEDEDEDYGQTRRSVTPHRRNRPSQRGLVKFFKGKPRLLSEDLELKDYQQTGINWLNLLYHNQMSCILADDMGLGKTCQVIAFIAYLKQINEPGPHLVVVPSSTLENWLREFQKFCPSLKIEPYYGSQQERANLREILERNAGQYDVVVTTYNLAAGNKYDISFLRNCNFNVVVYDEGHMLKNSLSERFSKLMRIQGNFRLLLTGTPLQNNLKELMSLLEFIMPSLFESKKEHLASIFKQRARTTDNNKDFNPLLAQEAINRAKTMMKPFILRRRKDQVLKHLPAKHRKVEMCELNSQQREIYEREIRLVMEHRQMVKDGVLPKDAKEKAKVQSSSSKNLIMALRKAALHPLLFRHLYSDEVIARMSDAILDEPEYSENGNRQYILEDMSYMTDFELHKLSCNFPDTLSKFQLSHDEWMQSGKIDKLCQLLHHIIVEKKEKVLIFSLFTQMLDILEMVLSTLKYKFLRLDGSTQVNDRQSLIDKFYEDDTIPVFILSTKAGGFGINLVCANNVVIFDQSFNPHDDRQAADRSHRVGQTKEVTITTLITRDSIEEKIFQLAKNKLDLDFHVSEDDKKAQDAIENKVSDILEDLIYDEAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X3C6</accession>
    <name>A0A8J2X3C6_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 14">DNA replication licensing factor MCM6</fullName>
            <ecNumber evidence="3 14">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BN860_04148g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3 14"/>
    <dbReference type="EMBL" id="HG316461">
        <property type="protein sequence ID" value="CDF90830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X3C6"/>
    <dbReference type="OrthoDB" id="1744952at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097373">
        <property type="term" value="C:MCM core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990518">
        <property type="term" value="F:single-stranded 3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902969">
        <property type="term" value="P:mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17757">
        <property type="entry name" value="MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.870:FF:000002">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000009">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000115">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.870">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008049">
        <property type="entry name" value="MCM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041024">
        <property type="entry name" value="Mcm6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF43">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18263">
        <property type="entry name" value="WHD_MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01662">
        <property type="entry name" value="MCMPROTEIN6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 14" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="16">
        <location>
            <begin position="503"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="204"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="826"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="224"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CDF90830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="999" mass="111023" checksum="C9D01F8304B53861" modified="2022-05-25" version="1">MNRASNSSPPPSSLGGGFGSSSGFENDSQTGSRNQFPSSSQIHAPSGVGPMSSSRFSSQNHFSDSQAGDGAPDLPMQGLRRRAINHVKKVDDMTGEKVREAFEQFLEDFSVEFEETGESDKVYLAQIEFMKIYDLNTIYIDYQHLLMRENGALAMAISEQYYRFLPFLLKGLRRIIRKYAPDLLTTGDTPAQFTQEVGEELRDSMGNSQNANSGGLSSGLGNNETGTATVSESNGSPEQTERVFQISFFNLPTVHKIRDIRSEKIGSLLSITGTVTRTSEVRPELYKASFTCDLCRAIVDNVEQAFKYMEPSFCPNPSCENKAFWTLNVSRSKFIDWQKARIQENANEIPTGSMPRTLDVILRDECVERAKPGDRCKFTGAEIVVPNVSQLGLPGVKPTATMDTRGISRNSESLNNGVTGLRGLGVRDLTYSISFLACNVVSVGSNIDSSKAESNSESGLQMANNLQATNLYQDYEKDQEVFLNSLNANEINELKDMVKDDHIYDKLVRSIAPAVFGHEPIKKGILLQMLGGVHKSTVEGIKLRGDINICVVGDPSTSKSQFLKYVTGFATRSVYTSGKASSAAGLTAAVVKDEEGGDYTIEAGALMLADNGICCIDEFDKMDISDQVAIHEAMEQQTISIAKAGIHATLNARTSILAAANPVGGRYNRKLSLRGNLNMTAPIMSRFDLFFVILDDCNEKIDTELASHIVDLHMKKDDAIHPPYNAEQLRRYIKYARTFKPILTEGARNFLIAKYKELRADDAQGFSRSSYRITVRQLESMIRLSEAIARANCVDEITPAFVAEAYDLLRQSIIRVDVEDVEIDDEDEADMQSNQEQPANAEQETEQSTEEASSANRTPFLTQPSSKEKSTTKVSYEKYVSMMNLIVHKIAEVERGDGRELTASDIVDWYLLQKENDLNTETEYWQERKLAFKVLKRLVKDRILMEIHGTRHELGIGSMDDPMEQPQQTNPDNVVYVIHPNCEMLETIDQEDSGEEQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X4W7</accession>
    <name>A0A8J2X4W7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="13">BN860_04610g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_04610g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X4W7"/>
    <dbReference type="OrthoDB" id="16982at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000014">
        <property type="entry name" value="Cation/H+ exchanger protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004713">
        <property type="entry name" value="CaH_exchang"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005185">
        <property type="entry name" value="YccF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503:SF10">
        <property type="entry name" value="VNX1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03733">
        <property type="entry name" value="YccF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="271"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="305"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="460"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="554"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="585"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="617"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="652"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="685"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="758"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="839"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="875"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="909"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="951"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="974"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="domain" description="Inner membrane component" evidence="12">
        <location>
            <begin position="270"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="11">
        <location>
            <begin position="588"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="11">
        <location>
            <begin position="837"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="66"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="369"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="799"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="405"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="426"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="803"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF87359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1007" mass="113387" checksum="FFD001A55012B188" modified="2022-05-25" version="1">MGGSKDRGLHALHSNGTVASGQDHVYRVFSVDDDPDEIEHDIKYLEGLHDGLKYALNGKVSKSGAAQNILPSSTDTSSSPKVGGSSKSKQEHQKQRGRQGQHSWQERRQEREPVQEQGQEEEREDERSHDQEHGHDGERQPSQVKIMAPANAPGDDKDDENSSESQVNPHEFLIEDMDNDSGSHRGLLIGEPGGEDEENGSGSDRASIADSLESYTLRERQHAINETHPFGIRIWKPALYKKHRSVQKVADEDIHETHLKSITWWVRIPNIIWSLSCGLFLFLVTSLVSLVVLVLGLFTESAREYSFLLLRLAYYLLWPFGKVVYLTFDEQYSQEDKDEGISAQQFYNWVTSYSNRLFFHRSQTQDTTPLLGDSYGDHQQLQQQQQQQQQQQQQQQQQHQHKPSYGSTDSNRGRQPQQGSTGSERSPNSYSQEDESSSPSSKTAIQRRYFGRGKWTFGRFVFYLCFHLVLQPIIFVFAFFTWLFVFTIPMSNILWNLTYHIRRHPLALGFKSVKSQTKPGETISSIKDSKSILLCTFRCAGWYYYKFTVDGTNVIVMNLVTLVFFTIFDFYVLKRVLGPSTWISHDTTIFALCLASIVPLAFYIGQAVASISAQTSMGLGAVINAFFSTVVEIFLYCVALRQSKGLMVEGSMIGSILGAVLLLPGLSMCGGAFKRKTQRYNPKSASVSSTMLIFSMIVMFVPTLLYEIYGGYVVKCDDDKRYDLSVTSIAKALSSQRCSFSHPALKFNKMYTHVIEPMSVSCAVVLALAYLIGLWFTLRTHAVMIWHFPISDQNKEGSRILEPSQSASGESTTNAGQETHVDEDGGHESPNWSRGKSTFILLFATVLYAIIAEVLVSCVDGVLEASSLNPKFLGLTILALVPNTTEFLNAISFAIHGNVALSMEIGSAYALQVCLLQIPTLVIFSIWYTWNMDHSMIDIRDQMFALVFPKWDLVASVASVFMFTYLYAEGKSNYFKGSVLILLYVIVIFGFYFQGLVDNWDSIEVNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X511</accession>
    <name>A0A8J2X511_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_06326g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_06326g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="GO" id="GO:1902494">
        <property type="term" value="C:catalytic complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:positive regulation of cellular component organization"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06890">
        <property type="entry name" value="PX_Bem1p"/>
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    </dbReference>
    <dbReference type="CDD" id="cd11879">
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        <property type="entry name" value="Protein kinase activator Bem1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Bem1/Scd2_PX"/>
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        <property type="entry name" value="Bem1/Scd2_SH3_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051228">
        <property type="entry name" value="NADPH_Oxidase/PX-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
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        <property type="entry name" value="PB1_dom"/>
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    <dbReference type="InterPro" id="IPR001683">
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    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706:SF2">
        <property type="entry name" value="SH3 AND PX DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3 MULTIPLE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PX"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="66"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="146"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="6">
        <location>
            <begin position="266"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="7">
        <location>
            <begin position="469"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="403"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="542" mass="60315" checksum="EE69C70A47A80A70" modified="2022-05-25" version="1">MLKGFKFSKRDSSGSKGRISSSDISSPTPTGASAGKFSKGSLSRNVSATSSHSVSSSRKNSRSVQTSEKMIRALSSYRSQKSGEVSFVEGELFVVLDEEREWYQALNPSTKKQGVVPKNYFEIVDKNRIASRHASIVSQQSEQPKMGTLYAIVLYDFQAEKSDELTSYAGENLFICAHHNYEWFIAKPIGRLGGPGLVPVEFVTIIDITSGYATGNDVKDDITFVNLPTVQEWKTNIAKYKASNISLGSVEHQPTATAQAHKSFGLDQNVVTKAAVESFGLEDEKYWFEVTCELSSGKGRRLKRCYQDFYDLQVRLLDSFPAESGKLRDPNGQWTRRIMPYIPGPVPYVTDTITKKRKDDLNVYVADLIMLPNYIARSEMVMSLFVVRDNGFDQEFPLVDGRSPANVGAERGEDTAVLTNPTEGLRSTGEDNTLTGEDLQLYEKLSELSLTNSKPHSRPPSTLPPSLKPTKIKFYYKDDIFALMLSSNITFAELCSKIGPRVDTEKFKLYVKLIGDEGEEVKNDEQVAQVIQGKLKISVHDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X529</accession>
    <name>A0A8J2X529_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_07008g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_07008g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4081733at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026248">
        <property type="entry name" value="Fyv8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02076">
        <property type="entry name" value="PROTEINFYV8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="608"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="290"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="610"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="773" mass="85137" checksum="052DFA581818F491" modified="2022-05-25" version="1">MSDQVGRKKSYRWVSASQASYDGSEWDSDDDGGYGGGYGSYGQEKDAKGSLERANTISKLPALPKLDYDGQREKVVEKANEEEEEIEPEIIGELPDLPGDIRVESFGGSDKPANYVNDTAESSSQEQQNLTGVKPSPLGLEVQEEPVIPNLEEKISGNSSKDSLSRRTPVNEDLDNLMAEISREMEQNAQEQSQEDELDWDDDLSASGEEGYGVSKSGYFTKLVESDDDDVQARDEEAVSIKTDDHALELEVYSPKTPLERNVLSTSLSYSQSSKETGLDSPKEEVQKRGSSPSSPEIIGSHAQLKSPVSKGSSLEESEDDALSYTESLDYHTSRNDAHEYSEKFQEVGGSESDSDESAMRIHKSGYFGKMVHDEGNDVETIHKEKAEDVGQTEDAPGNKNEGIEQGSQNAEEALQEDELDPHNKSIESKINAESEECGSDRNINKSISGKKSVNLGGWRPDTSAARSGFLEETARKAPPGYVYDEDGKLVDLTPSSMKPRVVSAYSEAESAWNPFPSNHNDELETIKDTKTLYDNQTIYNVPGLMTNNQNMPPLPNIDSQQHLANQPTGSNSSDPKSLTVGSSIATLSEIDSDKGPLQSHFKEILGGNDMHEESQTSKGTKESAPVKKSEIPKAYNVNGASAVPQLDLNKVISSKNSHASKIHELQRYYNQMLEHETGIEAWIEASLKTSAKPERGFLFEEYKVNKHVKDAYANADELSKKHTVSNTVASVNQNVSQLTKKVFSHPMKSRXXFFFGKVFVYLGIYTANCCFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5I9</accession>
    <name>A0A8J2X5I9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_16820g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_16820g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3231855at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013899">
        <property type="entry name" value="DUF1771"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053020">
        <property type="entry name" value="Smr_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47417">
        <property type="entry name" value="SMR DOMAIN-CONTAINING PROTEIN YPL199C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47417:SF1">
        <property type="entry name" value="SMR DOMAIN-CONTAINING PROTEIN YPL199C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08590">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01713">
        <property type="entry name" value="Smr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01162">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SMR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Smr" evidence="3">
        <location>
            <begin position="96"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="187"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="204"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF87891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="265" mass="29948" checksum="CF69E282D793947D" modified="2022-05-25" version="1">MSSGAAVERGLLLAHDYNHATDSEYHRLRNLADQAYSQRKKLSHESQIAYKKGQKAKAHELSEEAKRQQTKAENFNLQAAEYVFVQNNADSASDEIDLHGLYVKEAEWVLKRRIATAIQQGEPRLKVIVGKGLHSIDGIAKIKPAVEGLCDEANLRNYVDPKNAGLLIIELQGSNVPQSWSNPDVTGYHLIQPPSPTHPVSPIYQGPQQPQYYSQQQQQNQQQQQYYPQQQQQQQQQQQQQQQQQQXSGGNLLVQMLSLLCICLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5K2</accession>
    <name>A0A8J2X5K2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein CFT1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cleavage factor two protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Protein cft1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_17546g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">RNA-binding component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Involved in poly(A) site recognition. May be involved in coupling transcription termination and mRNA 3'-end formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CFT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6109at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000937">
        <property type="entry name" value="Cft1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="12">
        <location>
            <begin position="14"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="13">
        <location>
            <begin position="542"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="11">
        <location>
            <begin position="972"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="152"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="429"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="161"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="447"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF87923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1339" mass="152918" checksum="90EC944A43C119B6" modified="2022-05-25" version="1">MNIYDDILDATVVSLSATGHFTTSKYEELLAVKTNMISVYRVSLKGRLILTNEFKFQGKITDIATIPQKDSELDCLLLCTTLAKLSVVRYDELHNTVETLSLHYYEEKFKDISLLELTRESSMRIDPIKRCVVLFNNDVIALLPLLPLDLNEEEEEDGNEKEDGKGGEMEENAPRAKRPKPNKERFTAPSTIFPINHLHQDIRNVIDVQFLRNFTRPTLAIMYETRPVWAGNSQLTPFPITYIAFTLDITFNEISTEVKSTVIAVVKDLSWDFHTMIPINNGCILIGSNELAYIDNTGSLQSVILLNSYANKEIKKARILDRTKSQILLNKPISYSWSHSKERKEETLLMMDHEAAFYYILLDYEGRLLTKFDIINLPIVNDTLKDNFNPTCISRLNSTLSGNYIDLFVGFLSGNASVLRLNNLKSAVESREEHKETASPTEIDGEKFEDEDDLYSEEASDAGRNNESKEIVVETVSPFDIEVLSYLRNVGPITSLTSSKICSVEKFVKGLSNPNRNEMSLVATSGNFTGSHLTEIQISMRPEVQLALKFISITQMWNLKIKNKDKYLITTDSNKKKSDIYLIDKNFALYKEGRFRRDATTVSMSMFGGDKRIVQVTINHLYLYDTNFRRLTTMKFEYEVVHVSVMDPYILITVSRGDIKVYELDSKHKKKLFRVELPDILREMIITSGVILRSNMCNEFLTGLENSSEEQLLFTFVSADNQIIFFPREHNDRIFQLSGVDQLQEMLYISTYSLPDEVVPDPSIKQVMINKLGHNNKEEYLTILTFGGEVYQYKKSSRRHSRFYRNIAGGDLTITGAPDNAYAKGVSSIERVMHYIPEYNGCSVILITGNVPYIMVKEDDSVPRLFKFANIPLVSLTRWGKHSVMCVDDIKNARVYTLDRDNMYYGNKLPLRKINISNAMEDYMTLTRVTYHERSQMFIVCYAKEIEYNALGEDGVKLVGYKDNFPHAKSFKSGVLLVNPKTWNVIDKREFSDNSLINDARTMMIQLDSRTRRRKEYVIVGVAEVETEDLPSSGSLSVFDITEVVPEPGKPDTNFKLSEVFKEDIRGIVSSVCDISGRFLINQSQKVIVRDVQEDNSVVPVAFLDIPVFVTDVKSFGNFLIIGDSMQGFQFIGFDAEPYRMIPLGRSVSKLETVSLEFLVNGGDIFFAVTDTKDILHIFKYAPDEPNSLSGQRLVHCTSFNLHSTNTCMVLLPRNEEFAVQEKSLSPVQVIGGQSDGSVFKLVPLREDAYRRLYVLQQQITEKEVQLGGLNPRMERLSNEFYQSTHAVRPMLDFNVIRRFNTLSVEKRKVMAQKAGRRAHFEIWRDLINIEFSLRSLCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5M0</accession>
    <name>A0A8J2X5M0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BN860_18602g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_18602g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5M0"/>
    <dbReference type="OrthoDB" id="2354757at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035838">
        <property type="term" value="C:growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051380">
        <property type="entry name" value="pH-response_reg_palI/RIM9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28013">
        <property type="entry name" value="PROTEIN DCV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28013:SF3">
        <property type="entry name" value="PROTEIN DCV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035301433" evidence="6">
        <location>
            <begin position="20"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF87968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="229" mass="25453" checksum="862C14FD3CC3DCBC" modified="2022-05-25" version="1">MHFAVPLCLILITVSLVFQILSLVSTPISSISLSSYSGLNYGVFGYCDESTDSCSPRKLGYTPHVELTSSGSHRQVALPSSLRFSVTKLLVVHPLSFAVTLILYSLILLMNHRHLASSTAFLLTVAFFSLPTFLFSLLCFLVDIMLFSSHLRWPGWLMLPSTILIAVCCSLIWNVRRTVSIKNYEALQEARTAASVETYSMSDWPVHEPVAPNNPLLEPEVTYTSTFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5N3</accession>
    <name>A0A8J2X5N3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pre-mRNA-splicing factor SLT11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_17216g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in pre-mRNA splicing. Facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome. Binds to RNA.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5N3"/>
    <dbReference type="OrthoDB" id="10259600at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048995">
        <property type="entry name" value="STL11/RBM22-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF6">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21369">
        <property type="entry name" value="STL11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="STL11/RBM22-like N-terminal" evidence="5">
        <location>
            <begin position="4"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="249"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="257"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF87909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="279" mass="31473" checksum="8AA1744BD0628557" modified="2022-05-25" version="1">MEVQVCEKCLRDTKHLTKYPQGAECKICTGAFDMYALKQRSSLTKTLICKKCAQQRNICQCCMLDLVWGVPVELRDRLLSLVHDDPSLKTEEAKNEMMRRFLSFKDVKLGGAQITGNKEALEELEPLLALHRSFRLSFKSSHRYLLYNIDPSIPEWQISSSIEDLAGPNSVEALSVNHKAHIAALTLKRDDDKFASSIDKITTSQGLTRGQLTIDRFQVLVVPLIAPIDVNQFSAKVALSLQKLVFAESSRREPQGKHARPPKPGRVTKKSKRARDLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X619</accession>
    <name>A0A8J2X619_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_10682g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_10682g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X619"/>
    <dbReference type="OrthoDB" id="2421129at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17041">
        <property type="entry name" value="Ubl_WDR48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051246">
        <property type="entry name" value="WDR48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021772">
        <property type="entry name" value="WDR48/Bun107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19862">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19862:SF14">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11816">
        <property type="entry name" value="DUF3337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="545"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="635"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="798"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="545"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="581"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="644"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="657"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="798"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="810"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="822"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF87624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1015" mass="114395" checksum="91AA9F1F88B467E1" modified="2022-05-25" version="1">MSELTVSYGLLPPQSRCSQNLHILPISKILYPYDRDDYFITCGRDGSVIRHRCTTEGVSLGGTKVQAHNDWVSDIIQQDVDSFITVSYDFSIVLLTWRPELENWETKIIGDHEDYIKCIVEIPTDDDDGSFTFATGGLDKKVKIWKLDQNQAQCVHVFDNAQANDTGSIYCMNSVQRGKFLPFDLIVGDSNGDLVFYSCKQRTEFSRVKNIHKTNMKTLKMFDDCTKLVSTCSDGLICVWDLTEVVNVLKIMASFKWDCSIWCIEGDCLEELYVGDAKGRITRADFSDVQNVRLEMVYESHSAMEGIDATVNNGISSKESKKKHVGIVCINWLPNNFLLFSRSVDSNLNRLDLANNQVTVSEGGIALTKSSLLTNRRHVITENTKGEIQRWDLVVCELLDTFDPSEGNFDQVVSKYTSQEILSHWCSVSVKVGLLFVKLNKRILNTEVYGTALKGYDVVNGVQPNPDERYNLGKIVANSLFNEFVMFEMHKDKLYRQCLVPKKRNNSFFHNEVTPVGETQDFKCKDRRRKSAFYKISYPNSVTEKGTLGSLSAPNSPFLASQTPAPPKDRPLLGPPALSDRGTTSLTKNNLENGEGANFPPSKGSEGRTMSSGSLLSRKLRIFKNTNNRAFTATSAAADSAISDAEDSALDDDTKEIGNTTSETIGNNAGYHTENAQQQMYNCDTNIATTNDTTETGPTNSDSKKKIKEESLSDLIIQFHENYKQQVTNNTSTLKMLAKKPPQTKIVRDNLSPIVRVTNGVLIVVHSWEKGSCGGRVLFSTYLPPRNTDYMKALDKENAEDDEDIDDSLLEPLPQPQQQERYSGETEKAGEEDFTENEYTNGKNRKQVYEQLEGILPYWFSKILFCDSRVVKQQPKLNFVITPWRSSEPGGLQVGQPQPQQSPLQHYKMKFGRVASRSTDLLVGTTDLPRVSESNLKLLAPGMIKVKKIKAYIVDRFETRTPEMKSKLEPSEWLELLCKGQVLDNDMTLSTVRTLYWKSQSDIVFEYRRRMAVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X689</accession>
    <name>A0A8J2X689_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_13322g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_13322g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X689"/>
    <dbReference type="OrthoDB" id="10251230at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="649" mass="73118" checksum="2E0EEF85F2561A61" modified="2022-05-25" version="1">MVVGAQLTDEVSLRDMQIAAILRMLFLNKDTTDTNLESSFNDQELQWKVLVLDSKSTAIISSVLRVNDLLKAGVTVHALIQQRRASLPDVPAIYFVSPTQINIDKIVEDLKEDKYCDFYVNFTSTISRDLLEDFAKQVSQTGRADRIKQVFDQYLDFVVTEPELFSLELPGTYSLLNNPNSSEEVITSSCDLVADGLFNAVMTTNSVPIIRAPRGGPAEIVAEKLGGKLRDYVINTRSSNTSSALDSESLERCVLVILDRSIDFACMFAHSWIYQCMVFDVFKLSRNTITIPLKSEQGQEQESQKKYDLEPDDFFWNENSHLPFPEAAENVETALSAYKTEASEITKRTGVSNLTDLDPNSNSDTVQMQEVVKRLPELTARKNIIDIHMNIFAALLSQLESKSLDTFFEVEQDPDNTKTRSRFMDILKDGKTANMEDKLRTFVVLYLTSEHGLPKEFVKEVEKYFADNEYDISALTYVYKLRDFMQLSNRTLQNKSLEDGSSKATTGNNLSLSGLYGLTEGRLQGGVGSLISGIKKLLPEKQTIPITNVVEAIMDPLNSSQKNLETTDAYLYFDPRITRGSHTRKPKRQSYNKSLVFVIGGGNYFEYQNLQEWAHSQLHNPKKLMYGSTDIVTPGDFLKEIAELGSLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6F7</accession>
    <name>A0A8J2X6F7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histone acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_08724g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21992"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:21993"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="2"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6F7"/>
    <dbReference type="OrthoDB" id="3361892at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032931">
        <property type="term" value="F:histone H3K56 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051236">
        <property type="entry name" value="HAT_RTT109-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013178">
        <property type="entry name" value="Histone_AcTrfase_Rtt109/CBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016849">
        <property type="entry name" value="Rtt109"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571:SF2">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE RTT109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08214">
        <property type="entry name" value="HAT_KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027124">
        <property type="entry name" value="Histone_acetylase_Rtt109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01250">
        <property type="entry name" value="KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51728">
        <property type="entry name" value="RTT109_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF88373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="432" mass="49814" checksum="FC41C99204FB7811" modified="2022-05-25" version="1">MNQIHPQGLPKLLASCLPQDERFFVLQLQSVPTETHPIVTPKAKCESPLTVKVQHFFALFHHEKIVYALEVYVYLTLWFDSDCSAERLIFISKADTTGYCDVKLSFKELTKTLIEYLTSIDPNYYLHRIKPRGRNYVQQPELLTHRTNPIHALRILSKRHRALSYSKPEVPSDFYLSLQFPVKFETAICLFTRPEPQYLFAESSKNPNKHCLNGVQLLNWWLSILDDILCHVFESTSQAKLQIPGESTAQVRRHLDSVQFGNWQVGYVFGKNSSDLAAYTIPLLPDDPKTRFLRQLAEEGRINIVDLETFWIELAERQEFRLSETVSVMGVRGIPSKLSTHVPSSDDVFTAPSKKRFDYIKSYITGEEYDTEEGAFEAFANIRDFFSLRLNRSLQMVVGTMSSIDKEKFKRPVNPEEAHSVTTLVTRKKPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6N5</accession>
    <name>A0A8J2X6N5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_14708g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6N5"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14127">
        <property type="entry name" value="STKc_CK1_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000968">
        <property type="entry name" value="Casein kinase I, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="75"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="376"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="478"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="402"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="481"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="523"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="543"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF88629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="574" mass="63464" checksum="51EDEF94E0AEF250" modified="2022-05-25" version="1">MAAATNTAMAVNSLTNTGNMNLNRTSNVNSAANVNMANGAGAAGAGVIPTAANGGGGTMSSSSTRDDSTIVGLHYKIGKKIGEGSFGVLFEGTNMINGVPVAIKFEPRKTEAPQLKDEYRTYKILTGTPGIPQAYYFGQEGLHNILVIDLLGPSLEDLFDWCGRQFSVKTVVQVAVQMITLIEDLHAHDLIYRDIKPDNFLIGRPGQPDENKVHLIDFGMAKQYRDPKTKQHIPYREKKSLSGTARYMSINTHLGREQSRRDDMEAMGHVFFYFLRGQLPWQGLKAPNNKQKYEKIGEKKRTTNVYDLSQGLPIQFGRYLEVVRNLSFEETPDYEGYRRLLLSVLDDLGQTADDEYDWMKLNGGRGWDLAINKKPNLHGYGHPNPPNEKTRRHRSKMAGAGLHHQIGQSPQQQQQQQQQQQQQHQQHMLQHAQLSSQAQQMHMNGGALAPEDALNRGAAGQNGAAAAQFGSAKLDPTSYEAYQQQTQRKYAQQQQQQQQGATPGTAQTRYPAARGANGYPSGTAPQQATNKYNYQKQPRALPQENSGASGRQQKNTDTASFDTSKGFFSKLGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6Q1</accession>
    <name>A0A8J2X6Q1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BN860_12640g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_12640g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6Q1"/>
    <dbReference type="OrthoDB" id="627262at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF0">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="294"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="431"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="463"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="553"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="637"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="686"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="195"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="196"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="714" mass="78540" checksum="9C79421F4594D65E" modified="2022-05-25" version="1">MSETQVPVSWGKKPGIQQMTMRATVVGLVIGSLVLISNFQFGLQTGWVSMMSLPSALLACWFFKQVWPLIRPHDVPFTDVETVYVQSMAVAVGTGPLAFGFVGVIPAIEKFLTKEESGNSRNQGELFSIGQLLMWSTGLAFFGIFIAVPLRKQVIVREKLPFPSGSATATLIAVLNGSEILQEVSRSDVLQMRNRRLSESSPADREISSLSRGGSPDDRYSSLEMDDTVEGQQDVATYDENIGILFRTFVVSSLYTIGSYFLPVLRSIPLFGTYLSKNYLWEFQPSPAYIGQGIIMGLPTVSYMLFGCMLGWGILAPLARRAGWVPPDAGVDDWEKGVHGWILWSSLSIMVVDSVVGFIVVTMRSIVRFWLTDDKARLLTNVWDDSYESMLLEEERVINKRRYTTSSSSNRPNTVKLVYDDVDHEVAEEHLVTYTTVLSGIIVSSLLCIILVIYLFGLEIIPIYAMVTALLIALFLSVLGIRALGETDLNPVSGIGKISQLIFALIVPRSRKGAVLLNLVAGGVAESGAQQAGDLMQDLKTGHLLGASPRAQFVAQIVGAIWSIVLSSVMYICYNKVYHIPDKQFRIPTAIVWIDCARLVTGQGLPEKALECSIILGCAFALLSLVRNCYRDRNRKWLVYVPSGVAVGVGIYNTPSFTIARFIGGVVASLWMNSQHFNLGAKTKMIVFSSGLVLGEGICSIFNMLFTDLNIPHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6Q2</accession>
    <name>A0A8J2X6Q2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_15434g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_15434g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6Q2"/>
    <dbReference type="OrthoDB" id="4076669at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022757">
        <property type="entry name" value="Gsf2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11055">
        <property type="entry name" value="Gsf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF88659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="408" mass="47674" checksum="F037FDA3C0B5F647" modified="2022-05-25" version="1">MEVNVRLNADIEKDFAFQVDHEDTIKTKIARIFRKDGTGMGHLMVLRPTVFHKEEPTGFYKSMHPGYMTEGGCLIFDYDADEREYLQLLEEDRPILEQVWPGQLVLPRWEISKSNIYRYVAIMLAWLYTDLPDCISPTPGICMTNNMSRLIIPLLQHFELFDFADRMRQEITPGYSSLFAQWAFFSLHIGKILLITLFFGVGLCNPISFNPYKVIKVTQLDLAQPKIKNLVKSLGWVGIRRGTFDEYQSHFYQYITKKYGGPVGASKAGKLRMACNPGFPLGDGEGFQTPLSQRFELHTFEEIEKKGLFYISEDYFVELENNLKQNIEKCNGDIGLMNTEVKRFRRFGLFEPDAKIEHMVELRKKTYEKMHEEEESKIEKDRLEKVAKRRQEERRKQDKLANESKKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X795</accession>
    <name>A0A8J2X795_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BN860_10550g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_10550g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5529162at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070762">
        <property type="term" value="C:nuclear pore transmembrane ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056541">
        <property type="entry name" value="Ig-like_POM152"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056542">
        <property type="entry name" value="Ig-like_POM152_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056543">
        <property type="entry name" value="Ig-like_POM152_9th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056544">
        <property type="entry name" value="Ig_POM152"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037701">
        <property type="entry name" value="Pom152"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056540">
        <property type="entry name" value="TMD_POM152"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28206">
        <property type="entry name" value="NUCLEOPORIN POM152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28206:SF1">
        <property type="entry name" value="NUCLEOPORIN POM152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24312">
        <property type="entry name" value="Ig-like_POM152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24519">
        <property type="entry name" value="Ig-like_Pom152_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24527">
        <property type="entry name" value="Ig-like_Pom152_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23664">
        <property type="entry name" value="Ig_Pom152"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24097">
        <property type="entry name" value="TMD_POM152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 N-terminal transmembrane" evidence="3">
        <location>
            <begin position="78"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 first Ig-like" evidence="5">
        <location>
            <begin position="222"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 immunoglobulin-like" evidence="2">
        <location>
            <begin position="579"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 Ig-like" evidence="4">
        <location>
            <begin position="789"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 immunoglobulin-like" evidence="2">
        <location>
            <begin position="911"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 ninth Ig-like" evidence="6">
        <location>
            <begin position="1116"/>
            <end position="1188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF88450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1306" mass="147053" checksum="CFF3EE40D6E0946A" modified="2022-05-25" version="1">MDRDFLLNTATPRGNHWIGATSSYRSHVDTSAQRRFQPSTSSKEAKFNNLPEMKAKRSKDDIEVNPNSLPLISPEVLDVSRQRFLAFLVFLIIQGYKAYDLFLLKSGLPVVGLGPKSYWFNFISKYVVIDAMFLYFLPSFKIPKLTFPPWVICLQTVFMWGFTLFVSTEHDFTVITGIVTLLWRFNNKEMSLSGSSVSHRRLLDSSSHFKGAMTIKILPENTAMLNPLHESYCLPVDTNLLPEGHLKIPVRINSTAGIQSIQLEYRDLYTNEIELRNLTAKDFKVVNDPAFLLNKDHALAKGITESESIRYVELPLSQVGFYQIKRIIDSKNLSLKVYQSHLIISHCPVATILGTGSFDRCIGDSDKLSIELQGVPPMRLGYSKTVDGQKYTYMDSNLQPEYFESPLQSTPKHSFLSGELENLKWARTYPVTINLDLPVTQEGFYNYKIDSLVDGLGNVMDFRKIPESVKSAHGVSYNFKVHGIPRASLEEKFDPNSSTKKSILVKFESIQDWQAAIPYWAKLTHVAANGETHSFNLSTNSLTHDFQAELPGSYKLDSVQSKFCPGVVIGKSNVLVTKPIPPELEVKSTPILDQCVGQVGLNFDLTFTGIPPFHYRAMIYRIERGKRDLYDTKRYTSQGTRNQFNYNPTTEGNYEIVFDDLSNQLFTDPISLVPREKYTFKTSMRVKPSAVFKSTHKKKLCLGDHTNVPVQFNGEPPFTINYDILETSSNKRETFIEDNIESNDFSIGLPSFNMGGDYIVSLVSVKDFSGCVVSLSEPDVMIQVRRDVPSASFNLLENANSIKIKQGSFAEIPIKLAGEAPFTVKFQHLDYEGTVLGVYESRFNTNYKPSLNLNKEGFYKLIEVYDSYCMGKIENVEFQFEISFLDKPFFSVVENSKTSKLSALTFTKEQVCQGTESAIDLVLSGSPPFILEYAIIAPNGYTASDSIQVATRYATIKLPNEEAGEYTAVISGLFDTNYGESDFTKAIPSETEVLVRQTVNPSPYITFADLGKTFRTCSINVQEDASSLEPIKLKFIQGKPPFSITFSVYHESKGRSDQFTLNNVESTSFPYYKLYENLKLGNHVVSIEKIMDANGCINDMIGAQDNHILISITDVPKIHLLDPTAEYCVGDYVAYQLNGVAPFSIRYEFNEKQLKLKEHTSQFVRIASEPGTISIVSLKDSISQCVVNFTHPAMKKEFEKLSLLVHPLPSVTVSRGKNLIEDIHEGDQAEIIFTFEGNPPFSLTYVRTEEAEGSEGRGIPQVVETHKVTDIYAYEYRVVTSLQGTYQAIEVSDAFCFAKNDAYFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X7A6</accession>
    <name>A0A8J2X7A6_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">GPI ethanolamine phosphate transferase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_05930g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the GPI second mannose.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the PIGG/PIGN/PIGO family. PIGG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X7A6"/>
    <dbReference type="OrthoDB" id="272139at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051267">
        <property type="term" value="F:CP2 mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16024">
        <property type="entry name" value="GPI_EPT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000045">
        <property type="entry name" value="GPI ethanolamine phosphate transferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037674">
        <property type="entry name" value="PIG-G_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039527">
        <property type="entry name" value="PIGG/GPI7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045687">
        <property type="entry name" value="PIGG/GPI7_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23072:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23072">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19316">
        <property type="entry name" value="PIGO_PIGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 12" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035170873" description="GPI ethanolamine phosphate transferase 2" evidence="13">
        <location>
            <begin position="16"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="457"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="540"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="598"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="665"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="705"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="748"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 2 C-terminal" evidence="14">
        <location>
            <begin position="395"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF19316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF88960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="810" mass="91878" checksum="EB1C787E97F4C4BC" modified="2022-05-25" version="1">MLMKFLMLLLVQAIAVFIYCCGFFPQKNVLRGDAHFLLEPVLQNQSEPVFDKLVLVIIDALRSDFLFDQSMSNFTFVHSLLNSGEAWGYTAFSNPPTVTLPRLKGITTGSTPNFLDAILNVAEDDSSSNLKDQDSWLKQFAIHGKKMRFFGDDTWLKLFPLEFFQEFEGTNSFFVSDFEQVDWNVTRHLPDQLTTQRDWDVLILHYLGLDHIGHKGGARSIFMPAKQTEMDSIVKRLYENVDQNTLICIMGDHGMNDAGNHGGSSPGETSAGLVMMSKKLAQFPAPKAQRNISLPLTLPLQNDEVSYDYLTKVQQVDFVPTLASLFNVPIPKNNVGIIIRDFLPLLNTEVASIKVKENYHQMASLLGQNADINVEQMDQLFMLMKNLQNVLTKSATNYKYPILALGFGILVIATAVSLCFATTTMRLSPPFFLTLLMSLLLGLSTFGSSFVEEEHQIWWWVVTGVLLLSQFYLRTSFRTHFICLACVRLIRAWNNSGQKNTYNNTVFDILKANPQLQWYLNVLTIFVVGMRGTLGDTLSFIISFSLSGLCVSYKVSWAVVNHENVPGWLKELAFKNCLFFTSSDTNVFGEALVPMAQFFFQCVVVSIITKIVALRMGLTRSNIHNCISLFLMFQSPSPNIPLFLIFEVLQSALTQILIDHYSSNDYFISLTSLILQYFTFFQFGGTNSIASVDLSNAYNGVPENYNIYFVGFMMFVSNFAPTIYWSLYKSQFPDMNNHKWRSFAQRKITLLVYHCVVGCFLLAACYVFRSHLFIWSVFSPKLCYFAAWNIFMNLIVECGLESIIVATNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X7F1</accession>
    <name>A0A8J2X7F1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ZYBA0S03-09868g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_09868g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1854502at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120009">
        <property type="term" value="P:intermembrane lipid transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030011">
        <property type="term" value="P:maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13292">
        <property type="entry name" value="PH_Osh1p_Osh2p_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000061">
        <property type="entry name" value="Oxysterol binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000308">
        <property type="entry name" value="Oxysterol-binding family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000001">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000436">
        <property type="entry name" value="Swh1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF205">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="234"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="10">
        <location>
            <begin position="318"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="433"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="524"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="578"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="788"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="841"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1134"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="450"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="547"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="637"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="792"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="850"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="872"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="915"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="931"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="951"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="965"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="985"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1110"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1139"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1185"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1208"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1224"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1239"/>
            <end position="1249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF89130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1688" mass="190351" checksum="A70D4E2BC96E1DE7" modified="2022-05-25" version="1">MDRDSVAIIAPGGQSYSSSNIDPQMRSRTSSTSNDSSDHDMAVASKPLLKLKLLDSLRQGDFKQLDTLVRNEFQPEDDPNVEEVKNLMLHYAVQVAPTSLIKEIVNQWSNASSTSRKNAEKAGITIDINYRDQNGDTPLHLSAFQSRADVVEFLMDQPGINDCILNNSHLQPIEMCRNLGIAQMMQTKRATYVAEIAQEFRTAFNNRDFGHLEAILSNPRNAELLDINGTDPETGDTVLHEFVKKRDVIMCRWLLEHGADPFNRDRQGRLPIDLVGKVNENDSATNTKVAIDIELKKLLGKAAKEQSVIDVTNNLHEAPTHKGYLKKWTNFAQGYKLRWFILSIDGKLSYYKDQADTKNACRGSLNMSTCYLHLDSSEKLKFEIIGGSNGAIRWHLKANHPIETNRWVWAIQGAIRYAKDKELMMKSGTVPPSLAMSRGLNPGGRHESPLYSSQLQHSQGNRRASSQLDDELGMSEASQQGKAHSFAGNRNRMSSASLGSSELRLNDNLTESGKLYVNRMVGNRIDSGSHDMPSHTASNTSRRHSSQKSLLNNFPNGIASQDGNAKVNNYQRQAAGIQSDVDQQKGPAQEPNNSQTKEAVPKKPVNISNDFVAAKVNEPTEKSVDNSASNGAIDETSSNEDDLENTSEDADGDNRDQNSNFDVDDQEVKTQYGPYSQRLLMIQRSLSMELSSLNELLDSGHPNEEVWVTLKKSLPSLSNTFEKLYFLTSERDKKLGAMLTKQREVNNVWIQSVKDLELELIEKSNRLASLDKERRGLKRLLHKKLAEAGSSPDGSQFNSVQPRKLEDSNATLEGIAKFIEATKEEDEDSDADIFFDAEDLVDSSSEDENSGSQKSPEPQNFHSSYDSGALHAPDHMASHDTEAHSGDLRTSSESRENHQETEPSKKGFSIHEPPSRAPPDVPSNSPPPVPRTSNHFDQNMPTQRTLEQDMPDSHHSFDRARVASSAGSRGNPFRQQELQQGATHPNEHHHSENHLSEHHPSEHHPSEYHLSEHHPAEHHHSEHHHDSEYHPSEHHHSEHHHSEYHSNEPHSSEHVPSEHLPSEHHSSEHYSSEHSPGEHHSSEHHPSLHHHGEHHPSEHHHGEHHHGEHHHGEHHHDEHGDERDQYAYALKNKEHESSIHNNRISGSQSDRSRFSNSPADNDNSAEMKHKKRDDHTQENYPSGMKNDSSGSQRPISSYSQKKEPEDSAAVATGAAVGATAGNVSKHDSEKVPKKDLQDSKNSTPNSLKKSSSKLDEKSLPIQGVTEPQKEKSAIILREGSFLGYEDGLRKKLKLDEDDRPAISLWSVLKSMVGKDMTRMTLPVTFNEPTSLLQRVAEDLEYTDLLDMAASFDDPTLRLLYIAVFTASSYASTIKRVAKPFNPLLGETFEYSRPDKGYRFFTEQISHHPVISASWTESPKWDFWGESNVDTKFNGRSFGIKHHGLWYIKMRPNNGSPEELYTWKKPDNTVIGILVGNPQVDNHGEVVVRNHTTGDRCIINFKARGWRSSGAYEVTGEVFNKKNEKKWVLGGHWNDSIYAKMHTSKNKGDIMKTQDASSNGPKLDGSKFLVWKAAPRPEAPFHLTPFAITLNAPQPSLLPWLAPTDTRLRPDQRDMEEGLYDEAGDEKHRLEEKQRAKHRKRVENHETYKPLWFHKETHPITKAEYWQYNGEYWKLRKDHKLKNVSPDIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X7M3</accession>
    <name>A0A8J2X7M3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ZYBA0S03-12464g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_12464g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endomembrane system</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X7M3"/>
    <dbReference type="OrthoDB" id="1845386at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048284">
        <property type="term" value="P:organelle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16462">
        <property type="entry name" value="RING-H2_Pep3p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007810">
        <property type="entry name" value="Pep3/Vps18_beta-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058919">
        <property type="entry name" value="Pep3/Vps18_RING_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323:SF26">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 18 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05131">
        <property type="entry name" value="Pep3_Vps18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26148">
        <property type="entry name" value="VPS18_RING_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Pep3/Vps18 beta-propeller" evidence="6">
        <location>
            <begin position="1"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Pep3/Vps18 RING C-terminal" evidence="7">
        <location>
            <begin position="813"/>
            <end position="896"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF26148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF89238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="914" mass="105099" checksum="69E76EC4752F7EE8" modified="2022-05-25" version="1">MNVEIEHVQLELIKELQNNICALRVQANQLCFALRSGLLFLIDLDVPAAVSRCQVPLIDTGNNSEKLVDMWMAPDGTLLFIKTNFAKYYLCSVDTIRHEESTGNQKSGILAVKKLHKKNCNIKYVAWLDNSNFLCGDVNGQVFQVGVYDKHGKQRQDPEVHTVYQSNKAIDGILWCKDSGIIIASGNKIMYWNAITSGKMLKEPSETERFEQLQKEHGNKFSSYDNKFAWITKAGVVFGKLGATSNVLSNAKVLLNVELPESTHAIKDVILTEFHIVLLRGTTILIVNQFNNAIVFEDSIYSQKSERMLGLTADYSQEPPTFWCFSSSNIYEIILHHEAKAVWKLLCEQKEYHKALELQGLNSWEREKIYYDQGLHLMQVGEPLQAAESFGNTDCASVGSIALQFMQAPEKLEALQIYLTSKLNKLDAENQSQRILLSSWIVWNYMRQLNNIEEDINAERDSVNIRVLKEKKHLLVTHLHVFLKSHLDCLDKKTIYQIIAKQDRDQDLLYYAELIKDYEFVLSHWIKQANWYEALKVLLAMQDPESAYSYASVLLLNAPEETIHVWMQMASLYPVELIPALLIYFTSYQKQPSSIKQNFALIYLKWCVDEHDYTDPILYNTALYMLITGGDDKDESSEGRTIEFLDNHKAKYDSDFILRLSIRYHKVRTSIYLYTQLKLYKDAVDLALSSKMIDSAKLVVENSELDSNHKLKKHLWLEIARAMLYTDHGHQDIKHTVRSIISESHDLLEIKDLLPLLDEFTTIANLKDELIRSLEKHGQSMSSVSSQIQQSLKMKKDLATEIETFKDRYRILEPGVSCSSCEKPLQTRKFLVFPCNHSFHTDCLIKAILQSNDYNFKSKIENFQRRLARDRKSVKPEELESIITRKCCLCSDININTIDGSIAIEELEAAKWAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X7X5</accession>
    <name>A0A8J2X7X5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP-dependent RNA helicase SUV3, mitochondrial</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_05578g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X7X5"/>
    <dbReference type="OrthoDB" id="6692397at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045025">
        <property type="term" value="C:mitochondrial degradosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000965">
        <property type="term" value="P:mitochondrial RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17913">
        <property type="entry name" value="DEXQc_Suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18805">
        <property type="entry name" value="SF2_C_suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000269">
        <property type="entry name" value="ATP-dependent RNA helicase SUPV3L1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1080:FF:000004">
        <property type="entry name" value="SUV3p ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001549">
        <property type="entry name" value="SUV3p ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055206">
        <property type="entry name" value="DEXQc_SUV3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022192">
        <property type="entry name" value="SUV3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044774">
        <property type="entry name" value="Suv3_DEXQc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE SUPV3L1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22527">
        <property type="entry name" value="DEXQc_Suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12513">
        <property type="entry name" value="SUV3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="385"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF89493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="739" mass="84648" checksum="2B028B7EE5BA8CE2" modified="2022-05-25" version="1">MKHCRSILDTVHGSWLRTSCRLYHAKRHSLRIFEDHQWLVQTPRLDNLPPREKSLENGITFKFDGIQSGNIYAANNDFKHLLDSSMQHVYNRKVTASQQGQDNFKTHAWLKLRDHIYGQLKDEHLNEKIRKYQPTLNQLIVPSQPSFLIPQLVKMDDYGKVTWEKILKGQAVTKQSKFEYILTHTYDYIYEQEVIPLMMETRKTDGTDDIDISNPVEWFAEARKMKRHIIMHIGPTNSGKTYRALQQLKVANRGYYAGPLRLLAREIYDRFQHDGVRCNLLTGEEVINDLDAAGNPAGLTSGTVEMVPLTQDFDVVVLDEIQMVADPDRGWAWSNALLGVRAREVHICGEKSTLPLVRSIMKMTGDKLTINEYERLGKLKVENSVLPQGYSSLRKGDCVVAFSKKRILDLKLQIEKKTNLKVAVIYGSLPPETRIQQAHLFNSGDYDVMVASDAIGMGLNLSIDRVIFTTDVKFNGKEMVELTSSNIKQIGGRAGRFKSDSAGKETPQGFITTFDSEVLSTVRKGMEAPIEYLKSAVIWPTDEICGNIITKFPPGTPPSALLKAIALHLEKSTHKLFTMSDLKNKLNVLAIFEHMEDIPFFEKLRLSNAPVKDLPLVRNAFAQFCGTIARRQTRGLLSYPFPFEVLDYRCITDEKFGLELYESLYNIIMLYFWLRNRYPSYFVDSESATDLKYFCELIIFEKLDRLKKNPYNKGGPQSLGSIYKQKAVFLKQYTSRRYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X828</accession>
    <name>A0A8J2X828_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ZYBA0S03-09560g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_09560g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X828"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000177">
        <property type="entry name" value="MNN5p Alpha-1,2-mannosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF89118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="614" mass="69150" checksum="98A7DC7EBA87CDB0" modified="2022-05-25" version="1">MALVTRRYGKALRLAVIVLILCMIFVGTSKYSSDQVAVTEEYLRKYLSGGQLEYALESVAAHGSNAANAIKQGAGYIKPAPAGSDSPDLLPVDSENTAKLKSFYTEVFTHLKNFSPDGRSERQYKDSCPLDGDVGFRPENYHEWPKLARDSLASCLEVSEEEKDMLKKNHAGYVDVLSSLVLSKNAYKGRGIVTVGGGRFSLLALLVIQTLRELGTTLPVEVFIPPSDMGETQFCESVLPQYNAKCIYLSDILPSDVIDNSEFTGYQFKSLSLIASSFEDVLFLDADNFPVKPLDDVFDLEPYKSTQFVLWPDFWRRTTQPVYYDIAGIAVSTTKRVRNCFDDLTPPEVYTKNPKQLSDVPFHDFEGSIPDVSTESGQLMISKSLHLPTILLALYYNVNGPTWYYPMFSQRAAGEGDKETFLAAANFYGLPYYQVKTATGVEGYHRANGEGFRGVAMLQHDLNQDYVRYEAAADAIEAKYGGKTAKSIKYDKNYSLDKFYKTFFGSEDSPLKECDVMFAHSHAPKFDPYGLWTNNELVEDGKHFRSYTSLRKINNYDLELSAIGNIHKFVCVDKVKFKYLTEKLQSDQDFYDMCDYVAKRHEFLLSSHEQATTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8F4</accession>
    <name>A0A8J2X8F4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mediator of RNA polymerase II transcription subunit 13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_03092g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the SRB8-11 complex, which itself associates with the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 13 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF89846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="103819at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009401">
        <property type="entry name" value="Med13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021643">
        <property type="entry name" value="Mediator_Med13_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06333">
        <property type="entry name" value="Med13_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11597">
        <property type="entry name" value="Med13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0010">Activator</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med13 N-terminal" evidence="11">
        <location>
            <begin position="9"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator complex subunit Med13 C-terminal" evidence="10">
        <location>
            <begin position="1083"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator complex subunit Med13 C-terminal" evidence="10">
        <location>
            <begin position="1245"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="448"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="749"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="450"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="479"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="749"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF11597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF89846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1361" mass="152447" checksum="75F6FFD06DA33349" modified="2022-05-25" version="1">MATDQSTYNVDDLLSSFYRIEKLEKVNFHQYVPKKQEDQWSIQMELLLRKQNPRNLVALLSRELWCFSINDQEVPTPPRLSGDDNNEVLIPDKKGQFAADYSKPNLPPHYALFLKALRRMIYINITANSFNKLIPYGNACIFLEDSKSCHILLIEPHLFDIGVLATTITTKSPGLIPLTVDRIDDNFLRHHALYLAPSGIRMYLPSGNRQRFLARPPKNAEVLLMTLYVSHGIRLIEKKDLQWVKLIPHLGHLNGHTPNIASYMDSPTDSRTIVWPLDLCFAQKASDVHYDSKDSPCYHDLNDAFDIIEDFVQLKQTSAYRTPKSSNGALGSGLAGNSLSSGGGYTEQFQHYYRSQVGSAPSTSYLLSKENSDPKALSRDISPAYLSFDKPLSGSGENFGSGAFSTTPNLNESELFNDKKSLINDSEVSPVKSEVGSNLKHMLREGEAGTASTMTSPNQVISPSHSTGAITDSPGNEELFGDEDDDEDLFGDSNNYSGGENKSPHGTYIHKSKSDEITDDMFGMSEDEDQDRRSNSNENMYFFKDGRTSAMEPSLKKPSIKRKYLDIPIEEMTLSHTPLYTDPGAPLPIETPREKRKSVFAPLNFNPIIESNVDNKYKNGGKFSISPSHNDEALIFDISTADSSSSEEESDSSDDLEGLNIRQDMRSTDLTEQDAPFGGSISNFQPAVPQAFVRSELMSASLPRANDIMKDGANGIWKISHPDMSQQDPLLSTVNDNMVAADGSLAKMGTTTEQYPNSNKLQKDEPPSGLSENETSIPEPAAADTSLMPESSSSLPFLLRHMPLSSIPDVFFCSKATASITKKNQDILGLLAEQIVFDCGMFDSFGGQEVAYSALKDCENGLIFNTLQELFSEFTRIKGDDIISKIYRMSEPAIHVKRHHEVIKMNADSQVFSQYLDVKPAKGIKNFRFLMLTTSFFHECSSFVSTLSQTYINFEFGFCELLKLTNEDAQGLNFLKDFDESKLLLLAAQIVSYCSTNKNAGNNVPVMIILPIDSDGLEEIVTKTTKFEIIRNEVLSKIPNAQLFLKLILMDFVRNPLIPVDAYSNLCLSIYNILPPKTLKFTSIARTPPKKITFRTMQQSNSSSAIHYDAFIHLAYARSIDKEWVFAAFSDSTGNENIIKTLFVGKSRTKFDEACNEIWATALRLAGRKYGKICLILTRLNGVLPDDELMNWRRLSGRAVHLAVVCVDDNTKISFVDKDPSYPTFKLLLHHIGMEKTLHNSNFDDYEIRNIYEDVHGVIFRNSFPLSNSQHRCAIKSGALVRYKNCSGEQILDKFEVNLLNCPHSDSTKLLETILEEFRNLSALNSWFGISNGDVTHIPWHVLAVKKMMKNLVHTRVKILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8J0</accession>
    <name>A0A8J2X8J0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="3">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_07360g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="3"/>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10264149at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05167">
        <property type="entry name" value="PI4Kc_III_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.70:FF:000011">
        <property type="entry name" value="Phosphatidylinositol 4-kinase alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000022">
        <property type="entry name" value="Phosphatidylinositol 4-kinase stt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000014">
        <property type="entry name" value="Phosphatidylinositol 4-kinase STT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045495">
        <property type="entry name" value="PI4K_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF15">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19274">
        <property type="entry name" value="PI4K_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIK helical" evidence="9">
        <location>
            <begin position="1336"/>
            <end position="1521"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="8">
        <location>
            <begin position="1594"/>
            <end position="1875"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF90033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1891" mass="214991" checksum="CB584F41FFC8938D" modified="2022-05-25" version="1">MRFDVKRTVKNSVRAKALNKLSQLPTESLSEGRLRHLSQDLPVFRTHKEAKLYSIPLTLNEWEILLSLCQSTPQTLSQARDLLNDVVSSYFVESPRQRVSEVLDSMFKLEDLKHPNEILTFELTKFLLRVCTKFPALTRLCGPLIDQYMDNISQLFGETSSAAISLIGFINAFICEGNAIELSFLAWLRLSQALKRGGLLQTPGISNEFTDEVLVRYYDSGNEVSAPLFLDLLAQFQVSLASSIISVPKNGQSLLSELLLEAQLQLFEMHQNSATTPENLKLFLSGISNHTNLIIDMCTFALEYCSDVNMLDLSSETRALNSFEAQAHFIEFLCLVPFIDAVDGQLFTRFGVVVSDAIDRFLLSDAVTSRLVRSTVAAASLMNFYSEEFSLAFLKAFPMLVSSPHVETQDVAHVSKTFALGLKPLNEDAVVSTVYTINNFLTVYEDGAPVQPLRERHLTSNSLPPHQHSLLRKGRAVTSDTMQVIDNINHDMVMTNGKSHDSPNEDMATYHRFLFENCVTAVVVIASHYNDQSITALTVTILTQKVFVFSDELDETILKLLPLLAAHTTTTELNLLLTFYKLAYLQAGKRNKERLQNCIVLARVSMSRELLEKRFLSDMYIMMLRDLLDLIAACGEGEKSDHHRADTEITQFAEQIAIYLSPLAALMPKPGFPPLNLTSDEATTISFRNIWFNMVVHGFHYDSEIVKKHYSSLVVIAYNTPPLASDFPPTKREVSLEMNTILRRSSSSSNIKQQKHAISQFLQSSSVQNRNLSTSKIMFLASTLLLEMLRCDAGDCSQVLHYFCDPSVVRSSVDKAVGDINISIIEKYTFFVQSGNYRLADSQAVAKQLNNLLLRLPDRNPYAQAAAFQTCETFIKKIPSSLCHHHSLYTLLDLLTTLFDSVTDCEINRFEPHYEFLLKHSKNKILLPDSESWRKQTLTTLYNYSKHWLKIILAKCNQDTKILLQSYVSDVTYSDRLDDIEYGVSFAMEMAGTITVGDRELSKLTYTGRQKPNTIAKFISQHSWRSKSLVDTAIVSSPEDITHKIDIAVREMRRALKSGEPVIFEHVVSFLDSTSALLLLDKCAAGSLISDIVYIPFEVFTSPALKLATNVWLTITKERRDLANILLVAVGFRWMKSIDERKGLFSRKHDLVREENQPMLYSPYDKKAINRTAHDASQSLQPHHYIIQFFASHFEGTMFQSKSLLKMFTQWGIYGLKHLKYASLHPFARMLRNELLNFAVLLLTVNYKQNTRYVASLCQAIIDGALSWFVKPRSWPFGSNQLKIKADLATTIQLKNELDAVSSLMTRYSAAEFKLLQYFLMSEIMYIETWLAPMVKLKESDFEEPEADLIKVAFDKNPALAVGFLQRFPGKRQEEILTDLVVKNPLLCIGVPELLELYLTDKASQSQLDLHYVSYWCSVSPLKSINLFLSSWNRNTFVLQYSVFSLESHPVNVTFFYVPQIVQCLRFDHSGYVEKLILDTAKTSVLFAHQIVWNMLANSYKDDEGLEEDELKPTLDRVRNRMISTFDDKEREFYEREFGFFNEVTSISGKLKPYIKKSKAEKKQKIDEEMQKIQVKPDVYLPSNPDGVVIDIDRKSGKPLQSHAKAPFMATFKIKKRVEDSETGKEKEIEKWQAAIFKVGDDCRQDVLALQLISMFRTIWSNIALDVYVYPYRVTATAAGCGVIDVLPNSISRDMLGREAVNGLYEYFITKFGDEGTIEFQNARNNFVKSLAGYSVISYLLQFKDRHNGNIMYDDQGHCLHIDFGFIFDIVPGGVKFEAVPFKLTKEMVKVMGGSPDTQAYRDFEELCVKAYLGARPHMNAIIECVIPMLQSGLPCFKGLKTIKHLRNRFQPNKTEHEAALFMRTLIKKSYESLFTVGYDEFQKLTNGIPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8M3</accession>
    <name>A0A8J2X8M3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_16930g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 13">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="282270at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001193">
        <property type="term" value="P:maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
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        <property type="entry name" value="Zn-ribbon_RPB9"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
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        <property type="entry name" value="Zn_ribbon_RPB9"/>
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        <property type="entry name" value="Zn_ribbon_RPB9_C"/>
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        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
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        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB9"/>
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        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_TFIIS_1"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="14">
        <location>
            <begin position="71"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="11">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="10"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF87896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="122" mass="14356" checksum="0B72A2DB7B955850" modified="2022-05-25" version="1">MTTFRFCRDCNNMLYPREDNENQRLLFECRTCSYVEEAGNPLVYRHELITNIGETAGVVQDIGSDPTLPRSDRECPKCHSRENVFFQSQQRRKDTSMVLFFVCLACSHIFTSDEKNKRREFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8Q9</accession>
    <name>A0A8J2X8Q9_ZYGB2</name>
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        <submittedName>
            <fullName evidence="9">ZYBA0S04-10704g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_10704g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
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                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ras GEF"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="305"/>
            <end position="373"/>
        </location>
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    <feature type="domain" description="N-terminal Ras-GEF" evidence="8">
        <location>
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            <end position="980"/>
        </location>
    </feature>
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            <end position="1378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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            <begin position="120"/>
            <end position="140"/>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CDF89706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1413" mass="161495" checksum="0FA3A8FB3B80150F" modified="2022-05-25" version="1">MASKVEADTPLFMQDETDFISPTRPLHYSMKKRDRDPNVLPRHASELQTNYLPHSSYAMTKESSEMMSTTTKELPIPRQTFAFPQRDFNSDNTPVLNNNDNVPIEDILTPKVNNGESYMTESVYSEDSSDEDSSSEGSVSYSYGPIEIGGEPFAGLGLEGVNDNISVSSNVEIAKTAMVISSKSSPVRAIRTSAIVNGKGNLSPFSNQSFFIENIDNNFQKYNNEQSQYSLISGDFQGAQQSHSNSPIPKEPKSSIIDIARNTMTEIPKESQSSVVNMSRNNSSSIQRNTTLKKNTELKLDNENFAYLFIIAIHSFNSKTLDNPDDISICLSFEKDDIAFVHTVDESGWGEVTIVRNRQRGWVPFNYFSDTVKCKNIKEPGDHLKCLIETRSPLQTLLSASARFLLDCQSTSSSDDLNAITFNFQYINLVKDGVKKILEKTECVSRSNELVRQRPLIRKARKQLLADWYNLMIKADYYKHSTIPKNIQTLTELLYEVLDQAFVFFRTWSKERIEYEKEEHNKESNGGEVKGAEGSVYLRHHAVQYLTEAPTAMIRLQELYDMLFLYAGLILGRLDLIEHNPSGAEMLEAIVHQIIILLRELLYISKSCSSIIQDKFQYAYDDTVHKNLDPLLSLVSELVSCVKLLVVDTLREGFGIQDAELLVREDLYHHTDQGQRLIFIVSSLTTLIANTVSGCNSYLRLIGDFQLSKDRKFSDLQEMKITPEKFISHCSEGLTKNIDEEKLAKCMNKAIQEQNGAGVCHRELTRFSTIRAGNEKKMGFTIEGTQFLQELFMERKPFDRESKFEPFKLNLEEGSTAVDELDDINNKDSMSKQLMFGKDGSLIGASFKALVYRLTNEMNKPDKFFTASFLLNFRSFGTTVDLIDSLIARFDLNDKSIQYELHEKNGQYSSRASRLKNRRKLVCKVFQYWMESYWNYSSDYMVLPTLINFFNEGLSSFLPIEAKTLTEIAARLCTLTPHSKEFKKDTENLRQLHPLSIQHSRTCSIYSELSSSSSTSHRSSTVDQQMLEEYDLTHIPSQNRNSMSLPLPVLNYGTCPLLSKRNIQDMERLLISYRLVTGYSTSAMNKSPNDLNAQNDTRLLILEWNELVTSDLRVSQPLAHNDMNLVDVNPLELAKQLTLIESQLFMAVGPFELLDENYLPKKHHLGLAPNVNAIVNFTNQLSNYVIECILYPNISLKERTSRLQGWLKIALAASHFRNFNSLAAIMTALQNHAITRLQSIWDGLGDKEIDLYDYLARVVHPNNNFKIYRKKLKKYTEENKFGEEQHSKTIVPVVPFFNLFLQDLTFINEGNANSRDDDKFRPYTLVNIDKYFKITKTLNIINFFQVDYESTAQGTDKLISFFNIGDQIDEESTNIKPIPLLQEFILCEFWRVNTLFKENKDRGYQLSIALKPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8R2</accession>
    <name>A0A8J2X8R2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ZYBA0S06-03246g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_03246g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4063069at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF90209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1127" mass="128288" checksum="3911808C6D0A5FF7" modified="2022-05-25" version="1">MEEHVVLGRLHTSRNVVECFLMKNSFLVLIEREQIKKALYTDDLKHDDMKIVFSRGYNVAASIYWEPLTGEEYLFVLKDCGALEILDSHCKRIDLLETNIELDDTNKFILFNNKNQIYVNLTKSSIYAIEYQVKNRGISFVKKDETLKCIFEDISTVIGMDFQWNIDFYTGREYPVIAVLLASNISGQINFQAIRQNGTKNGPWQNIVNRATLDFNESHDSADQRPILKSISNVGFFVFFATRVLFIKMPNGYDQTVGGNSFNSYGPFDAMSLRYNSIEMEENKINRSGDLMMKRNAASLEFWFFTLGSELLKFKLVIELEDPDELIMHWSKLTCENFPVVGLKQNESNGGFLQRSIFLSRTVAILLNDSIGIMFVDLEKVRLVNEMRFHLSQFFYSDTIGGDFRRTISCGASAENAGFIEYKFWGYSDIFAPIKSFTSEEEIVELWNSEQGIWWKNSNGQIFHEGQLIETFLPPVHVTSQGVVLRDTSIAVWTNIINDFEGNYSYVSKNGEIGWSDDLEKVFLENLKSSALAKYILSCSRLNDGSKITILSVDNKVTVLENSEIIKKNVDLSRYLDSLSSVSIVQCQQTYRILVCDIGGQLCELDSSSLNVCCKLKFGTKKLQISVVPESTFCILYNKDDVMLMDSAMNGIQITKLDVRYRLRKIVAKSSCDIACLDEGHNIYQFRIPTSLNSPQTISKIFASNTHIHTKVLSLPCSTRYVITSSLCSGHSNVLKRLKYYAELQLHDLGTQETVFRYDLSNEYPQAIISDMVAFSFHKPTFEDYSPQSNLFAMQLAFDRIFIVSLNYEMAEEESLDNLLLFSLDDGNGSIHFLQGINTGYSISALHAYDSRVVLAAGHVLQAYQIDYTVKEDAFKIITVSNEVYTGGLIKNFIEFPNFFSLAPQTKRLKIQKRNTDLRNNKKRVAAFNLVKGLQKFDLTWSSRDQQVSLRIVPVVDVGQAPFFSVDTSERLMVAFSVCRMDASTWLAVAFSNKSLLLYCIHGSDEPSSLQMKLPDQVTGIFSAEKPHCFSHVRKKESLINFKRRSMSILFVLVTSKGKYGIGKTESSEFSKKLESTCVEEMTEQFKFIRLENGESDLRFTDARILPPDGGTKXXXFFFGINYASKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8V6</accession>
    <name>A0A8J2X8V6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_02366g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_02366g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8V6"/>
    <dbReference type="OrthoDB" id="442460at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070139">
        <property type="term" value="F:SUMO-specific endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051947">
        <property type="entry name" value="Sentrin-specific_protease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46896:SF3">
        <property type="entry name" value="FI06413P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46896">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="7">
        <location>
            <begin position="419"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="690"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="756"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="853"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="957"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="992"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="48"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="692"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="855"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1000"/>
            <end position="1015"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF88102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1015" mass="114639" checksum="F09D5A86A2FBF00E" modified="2022-05-25" version="1">MSNKRRRIQGSLPINTLSSTLNGKGSGPEGDSGSGQKIDLTTPGQPRRYKEFRKDPRVVEEVNHSSTKNFLPLNDRKVSSKSKDADQTEDHLGLVKETPLRRSTRRVNGLLFSSPIESEAHFSSTAATIIKLNLSGSLRTLDVRKQWDEKAQCLMKFIADSDGPRIGFTHRNFEIKNAQVEFLRDCELIIFDKGFSCIGLVLKESRFIDMDNKSKIGRAHTRLFMWMSSADGKDGKIAKIKDIVVESDSCKTKLVGSKALVVEKLDQAAIQDYKTKNCVKTTNEAFEKLKKTNYWDKKDFQSLGHQKRLSQFTSSASHKIHVPKTTVIHDLHESAIPHISTTDFFNKSSTPYIKNKRTNENVDSKKDPVEELRMSGRTRSQSPALRQSTLWDLDSEKDFETPEVFKPKLHYKFADGTSYTVTNQDFKCLYNHDWINDSILDFFTKYYAESSIKKGIVGRDDVCIMSSFFYTKLVSDPNDYYGNVKKWVNNSNLFKKKFVVVPINSTFHWFGCVITNLDVLYNHFKEIDRNSHGSSHALQAINDKSIDHTFSRNDDELTVSTPIVKILTFDSLRQTHGREIDPIKEFLISYAKDRYSMNIDKALIKMKTCVVPQQPNMSDCGVHVILNTKRFFEDPVATMEVWRSTKTRSKASTRIVNEYFDKASRDRARKDLRNVLWDLLQMQIQLMHERNEDIDEKDSGTNDEDENDGDLEIIENYPQDEEQTFSTKSANENVITATGAQETKLDSLSKKTVISDTSTTAAPKHSANDHGDNNDGLVIDRASSLCDSTRSASQRNVLKSSPLRDIVRCDYKQSTKGVSSPYFGESHLTKRAASFGYEAGDTFFSPTLSSFVDSQRSDADMDRTTRSNSSEDAGSPIMLTNGVSNCRRFPTSSGHSREGKNSPSSPFNETVIVSDLEQDEDVNLVGGTQSEKGKSDDDDLTHISKAEKKSIENISLSSDSLNRPNTRIGRTISSKKDTQLIDDDSSYVEHNEDKPASFLIMSQNDAQAITSDEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X926</accession>
    <name>A0A8J2X926_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ZYBA0S05-07030g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_07030g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X926"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032161">
        <property type="term" value="C:cleavage apparatus septin structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000162">
        <property type="entry name" value="septin-7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="9">
        <location>
            <begin position="113"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="490"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="36"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF90019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="520" mass="58765" checksum="1444A402811C2C6D" modified="2022-05-25" version="1">MSVREETVVIKKEESNYSMGTGIQGAPQSEQAPGSQGNANGSQEGAAGSASSTKTQPAANNFASNEYLSGSESPQLGQVLPDQPELKIIRRKIPGFVGFANLPKQWHRKSIRRGFTFNLLVVSQKGLGKSTLINTLFNKDLDSIGESNPNAVAEKLEGTGLEDGEENAASLHTNEGSGESNLHSNVKINAATTTIEENGVNLKLTVVDTPGFGDAIDNTDSWKPIVDEINLRFDQYLDSENRINRTFEDDNRIHACLYFIEPTAHLLKPLDIEFCKQVHEKCNLIPVIAKSDILTEEEINVFKRRIRRQLDEAQVQLFQPPQYSLDDEESIQASRELYEKLPYAVVGSTEVVVSRDGRQVRGRSYPWGVIEVDNSNHCDFVYLRDMLVSQYLEELRERTNKVLYEKYRSEKLTRLGIKQDNSVFKEFDPDMRQKEEKQLHEAKLAKLEAEMKTVFHQKVSEKEKKLQKSEAELFSRHKEMKEKLTKQLKALEDKKRQLESSVNNPAPPSPAQPKKKGFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9G5</accession>
    <name>A0A8J2X9G5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ZYBA0S07-00122g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_00122g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC12A transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X9G5"/>
    <dbReference type="OrthoDB" id="2020542at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015379">
        <property type="term" value="F:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055064">
        <property type="term" value="P:chloride ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055075">
        <property type="term" value="P:potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034486">
        <property type="term" value="P:vacuolar transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000013">
        <property type="entry name" value="Solute carrier family 12 member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018491">
        <property type="entry name" value="SLC12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004842">
        <property type="entry name" value="SLC12A_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827:SF72">
        <property type="entry name" value="GH08340P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03522">
        <property type="entry name" value="SLC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="348"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="417"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="475"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="56"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="10">
        <location>
            <begin position="542"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="906"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="917"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="931"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="951"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="982"/>
            <end position="996"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF90400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1094" mass="121929" checksum="B7A167C7A4289162" modified="2022-05-25" version="1">MSSSTGPQPQEHTSLLAPKQRYFNYEPDSTELRRKISSKYDPENPNKDKLGTYDGVFVPTALNVLSILMFLRFGFLLGQLGILCAFGLLLISYAINLLTTLSISAISTNGTVRGGGAYYMISRSLGPEFGGSIGLVFFLGQVFNAGMNAVGVVEPILYNFGYSLPYEPPAAIWEILPRGRWVEFGYATVILTICLGVALIGSQTVSRAGNVLFWILAASTASIPLSTFITQPFQHGDMAYTGPSWQTLKGNLLPRLTEGAAGSLLEGKETFNDLFGIMFPATAGIFAGAGMSSELRKPSKSIPKGTLWGLLFTFICYAFVALSMSCSIPRETFYRDVQIIQSVSAGPWIILMGELATSLFSIIMGVVGAAYVLEAIANDNIIPGVSVFHKRPLAAVLFTWILTQLCLFLDVNKIAVFITMTFLMTFAVMNLACFLLVISSAPNFRPSFKYFDRFTALTGTLMSTAAMFVVDGLSASAVILGMIIIFIMIHYLCPPKPWGDVSQSLIYHQVRKYLLRLRQDNIKYWRPQVLLLVDNPRTSWNLIRFCNNLKKGGLYILGHVTVTSNFQGQFQELRKQQKAWMSIRELTRIKAFVQIGIGPSLPWGVRNVFLGSGLGGMRPNITVVGFFDLKHYQEATRLDTASVQTNDASCDGYSSFDPTSKHLPTDDCKNEDKIKVQQWVEILEDLSLMHSNIAIAHAFQKLDMDQLKQGEPEDKKFIDLYPIQMCAKMKVQKDSPSVVTTNFDTYTLILQLGAILVTVPEWKRTHTLRVILFVEKNLDRNEEMRRMQNLLNVLRIEAEVLVVALDQFRVYNTIIKGDPINFEYVNSVMKHDAWWQDLVEARNTMKPKRRFSTVDEIQHKPYNIKSQPHKYKLSKLQRLGVSFTMSSSMPHNASLLSDSLDEDAETVRESSPSIPSLKERMNYESTRRKNNNSVTRVSSTQRNYDDKSKTSSRPVFSSNKLPNTKVLEDQSGDKPTIVPVVESPSSGNEGSGSREPGLPELSSTYSGDSLLRAMQNLVFNDLPSRAQHLILNDMMSQISGGSDLIFSTLPVPALGTHEDEEASLRYVEDLHTWLQGLPAAMLINSQTMTVTTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9J8</accession>
    <name>A0A8J2X9J8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_15522g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_15522g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X9J8"/>
    <dbReference type="OrthoDB" id="64915at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055080">
        <property type="entry name" value="Gal80p-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051317">
        <property type="entry name" value="Gfo/Idh/MocA_oxidoreduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43708">
        <property type="entry name" value="CONSERVED EXPRESSED OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43708:SF1">
        <property type="entry name" value="GALACTOSE_LACTOSE METABOLISM REGULATORY PROTEIN GAL80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22685">
        <property type="entry name" value="Gal80p_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="18"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Gal80p-like C-terminal" evidence="2">
        <location>
            <begin position="156"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF88663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="431" mass="48479" checksum="5CED3E4968096FCA" modified="2022-05-25" version="1">MDHNKRSAVSTVPDAAPIRVGFIGLNWNKGWALKTHRPAISQLSSQFLITALYDEDQEAVVTTIQKLRLVHATAFTSLEAFSSFKNIDMIVVSIRASKQYEVLMSILKHSATNLSLKYLFIEWAFGSNLQQAQEIYDATAQRRLQTIVALQGRKSPYIQRAKELIHENYIGEINSVEIAGNACWFGYERSRKFAPGMFEMGNGLDLVTTTFGHTIDLLQYITESYFSKINALIFNNIPNQTLVDDLGNPMGRTVPKSVPDHLLFHGSLQRGNIPVACSIKGGKPTKKFTKNFVIDIHGTRGDIKLEGDAGFAEMSNLVLYYNGIRANDHPAAGEKLSSEQGKEFMEVYHLRNYDAVHGNIFRLYQSIADFHFNTRKIPNLPSHFIMQGFEFEGFPTLMDGLILRKLIDSVYRSSSLGSTLDVTNTLQFAFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9Q2</accession>
    <name>A0A8J2X9Q2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ZYBA0S08-03532g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_03532g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316461">
        <property type="protein sequence ID" value="CDF90806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X9Q2"/>
    <dbReference type="OrthoDB" id="427368at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022100">
        <property type="entry name" value="WDHD1/CFT4_beta-prop_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048591">
        <property type="entry name" value="WDHD1/CFT4_hel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19932">
        <property type="entry name" value="WD REPEAT AND HMG-BOX DNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19932:SF10">
        <property type="entry name" value="WD REPEAT AND HMG-BOX DNA-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20946">
        <property type="entry name" value="Ctf4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12341">
        <property type="entry name" value="Mcl1_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDHD1/CFT4 second beta-propeller" evidence="6">
        <location>
            <begin position="451"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="domain" description="WDHD1/CFT4 helical bundle" evidence="7">
        <location>
            <begin position="784"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="397"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="401"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF90806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="892" mass="100347" checksum="1B1691C262F549D9" modified="2022-05-25" version="1">MPEIVDKSVFDYGGKTMICLAPDNNHLCVANKNGLSKVLHTNSPEEEPEVVEIAKGITGIVCTSDTSYVLTTVQGDCYKYDFKKGTDQLLARSALPLRDCDVVHGGKMVVLGGDDLEIVLVELADDPKRHTLKIEEQVTQLSYSSQTNLLSVSMVNGKVSFFSLSSTRPNKLHEMNGYIPANSYNDTYRDKILNSLGTNLLSDDENDEDDGDENNVKDPEFCDENRISTRAAWHPRGLHFALPCGDYTVKVFTIKNYLLIKTLTNPAYSGAHYIDLKFDVLHGVYIAAVDLDNRLTVWDWETSGVIYKKEFQQKITNFVWKIQPDSEILDIILGTWSGDVITVKKVAEPQEGNSHEIPKRHGLFVESDNDEVSPYPTETLEAANEVFTDEENYNTKKHSYEDEENFIEDDDGAGYVSETRPLKQKKSVRNGSLPPSKASLSRAGSKPFVYKPLSQGATPFGSSDRRYLTMNNVGYVCVVRNNEQNSITVSFFDIGRFTEYHFEDLFGFDICSLNENGTLFGQSKTGHVHYRPHNSQHPSWTKILPLQKGEKLTSVSHTPKRVYAGTSSGYIRIFSQYGVPLMVEKMSPIVALAAQDYKLFSIHFHSSHGITYSLLERSPSSAKYYQRESPLPLTLPQENPQLSDNDSDFLYFNPLGIKNFFFSAYGDPCIFGIDNVLLVLSKWRSAAESRWLPVLDTNMEIWKTSGGKDSDVRAWPLGVIYDTLNCILVKGRNIWPEFPLPLPTELEMRIPIFVKSKLMEEQKDHINHGDELVEQEIVIPPQWAAEEEFLRSKVLSLLLGDTVANDGEIYGNEGEILDSLVGVHDKSLLRLFASACTEQNTEMALSLAQEVKQDRALNACLKIAERAELLNLSRKVGELREARIEQEINEGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9Q4</accession>
    <name>A0A8J2X9Q4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">BN860_02014g</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X9Q4"/>
    <dbReference type="OrthoDB" id="63989at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990604">
        <property type="term" value="C:IRE1-TRAF2-ASK1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070059">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036498">
        <property type="term" value="P:IRE1-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10422">
        <property type="entry name" value="RNase_Ire1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13982">
        <property type="entry name" value="STKc_IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000572">
        <property type="entry name" value="Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.180:FF:000002">
        <property type="entry name" value="Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000443">
        <property type="entry name" value="Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.180">
        <property type="entry name" value="KEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045133">
        <property type="entry name" value="IRE1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010513">
        <property type="entry name" value="KEN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038357">
        <property type="entry name" value="KEN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954">
        <property type="entry name" value="IRE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954:SF6">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06479">
        <property type="entry name" value="Ribonuc_2-5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00580">
        <property type="entry name" value="PUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51392">
        <property type="entry name" value="KEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 21" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="17" id="KW-0834">Unfolded protein response</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035237570" description="non-specific serine/threonine protein kinase" evidence="21">
        <location>
            <begin position="23"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="691"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="domain" description="KEN" evidence="23">
        <location>
            <begin position="1000"/>
            <end position="1131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="CDF88791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1131" mass="127808" checksum="99DEBB0BF418EBFD" modified="2022-05-25" version="1">MRLPQRTFYAAILTLWVSLCLGFRLWPQWADKGIDGGQDQSVDPVSPRMTNVEYHTAIPTAFRRQSQIAATIPHSSSVRWLQAPTEVAYNATFKKKKGDAYDFASGRSALIPYQYRRSLDKVSLSGILLATDIEGGLHALNRENGQLLWSIDSKHTRPLVNIVEPPRVKTKETLIIEPYGEGNIYYFNIFQGLQKLPVSIAQLVASSPMHLMTKMVVDDIGTIVEDEKMYTGSRSTAMYVIDATKGDIISAFGPGTENKKYRDNPETCTARGVEQEGCENVIIIGKTIYHLGIHSKDGTTYNVTYASWQQNTLDTRFASLSSLAGDSTYISSFRDRSLLAINADYKIAKWVSSKFPGIVNSIFDVFTDESAGENIIVPRPFRSPEDATFQKRKVYLSQIDNDSWFALSGENYPSLVEAAPAAKYTLSERWRVPTLFENEDLFTTAVTGVHTLRSRKFEQIINSSPSDKSLPSVPENGPLLIEPSPNLPLEMDDVSLNNALDAYISPQELEAYRLKVQEEIANEFMQKHRGSIIYLVGKLAYRVVESSLVLLFALFVLTVLQKFRIIPPIHSLLEKSGLISVEDIKVENLEITDEVDSEKRIHKDDQESPTLAGKNTIMNSSIVPTDEDEEAEIKAEEGNISKNNCPVIEKKKRKRGTRGGRKLKKKHGTQITEQLQHVETIEFENGLKNLTVSEKILGYGSSGTVVLQGSFQGRPVAVKRMLLDFCDIASQEIDLLTESDDHPNVVRYFCSEKTEKFLYIALELCNLTLEDLVEQKKPLENIPPIPRNYINILHQIASGVAHLHSLKIIHRDIKPQNILVAPPKKCVAGYKTESCNLRILISDFGLCKKLEADQSSFRTTLANTGGTSGWRAPELLHESTRKLLESISVDSEESNINGSTPTSPYDPATKQRLTKSIDIFSLGCVFYYILSKGEHPFGTRYLREANVIKNNYNLSGLHQTLKDRSLIYEANDLITQMINRDPLLRPSAMGVLNHPLFWTTSKKLDFLLKVSDRFEIEKRDPPSDLLLELEGHSKQVIPNKDWHSKFDKDFMENLGKYRKYNGERLMDLLRALRNKYHHFMDLPEDLAAVIGPVPDGFYDYFIKRFPNLLLETYHVVRQNLQGDQNLGNFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XA11</accession>
    <name>A0A8J2XA11_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ZYBA0S03-07778g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_07778g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="528"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="564"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="603"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="641"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="672"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="776"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1287"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1329"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1360"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1484"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="169"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="872"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF89040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1524" mass="171252" checksum="0941036BE360167A" modified="2022-05-25" version="1">MDPVDNLMEERRSAESTETIPAVSTPPVYGAEQAQNPHSHGNSEQDKQNSKDQDEDEDNGAALHEMLTRTRSGGQSSQLCPEVASRVRSLARTLSTRRLSDGPLEADTDEDGFNAERILKSFVHEAHQQGIDFRKTGVVMEDVTASGTDSSALEGHTFGDLLCLPLTVYRAVKAMRHRKMRKIIRDVNALAKPGEMVLVLGRPGSGCSSFLRTTAGVTDQFAGGVAGEISYDGIPQHTMMRHYRSDVIYNGELDVHFPYLTVRQTLDFAIACKTPAVRVNGVSRKEYISATRDLYATIFGLTQIYDTNVGDEFVRGVSGGQRKRVSIAEALAARGTVYCWDNATRGLDASTALEYAQAIRIMTNLLHSTAFVTIYQASENIYETFDKVTVLHTGRQIYYGSASAAKAYFERMGYECPPRQATAEFLTALTDPHGLHRVRSGYEGKVPRTADELEAYWRASPEFAALKNDIEEYKSAVNGEATEQFFSESMRAEKSKYARNKSYYTVSFPEQVRLCTIRGFQRVYGNRSYTVINVAAAVVQAFITGSAFYNSPSSTNGAFSRGGVLYFALLYYSLMGLANITFDHRPILQKHKTYSLYHPGAEALGSTLSGAPFRMIGLTCFLIILFFLSNLNRTAGSFFRIYFFLSLCSESIYSLFEFIAAACDNISQANSIAGIVMFSISMYSTYMIQYPNMHPWFQWISYVLPVRYAFEGMLEAEFHGRHMDCGGTLVPSGAGYENVANENRVCAFMGSQKGQSWVLGDDYLKSQYGYKYWHMWRNWGIMFIFLVGYIVVKAVITEIKRPVKGGGDALIFKKGTRRAAAKEPGPGDLEALDQMKLQGDAREKFSSGSDLMRGDATDAGAVDSSDEVFEGFQSKGIFIWREVCYTIPYMGSERVLLDHVTGYVAPGTMTALMGESGAGKTTLLNTLAQRNDIGVVTGDMLVNGHPIDTSFERRTGYVQQQDIHIAEMTVRESLVFSARLRRPQSVPDEEKLHSVENVIRLLDMEEYSEALVGALGCGLNVEQRKKLSIGVELVAKPDLLLFLDEPTSGLDSQSSWAIVQLLRKLARSGQCILCTIHQPSATLFERFDRLLLLRKGGQTVYFGDIGDNSSTVLSYFEGNGARKCEQSENPAEYILEVIGAGATATIEEDWHEVWQNSSERRETDEHVTQWIQELSSQAQEKSEIGEKPSKYATSYLYQFKYVWWRTTTMFWRDMPYLMSKMMLQVVSGLYIGWTFFNPGKSFTGLQDTLFAAFMSVIVSAPTMNQIQARALSSRESFEVRESKSNMFHWSLLLITQYLAEIPYQLLFSTMFFVAFYFPLRVFFEASRSAVFYLNYCIMFQLYYVALGLMILYMSPNLPSANVLMGLVLSMLIQFCGVTQPMSLMPGFWTFMWKASPYTYFIQNMVAIVLHKKPVVCKKKELSYFDPPQGKTCGQYMENFLKRSPGYISNPNATSNCAYCQVSVGDEYLAQRNVSYGNLWRNFGFYWAYIVFNIFAMVGIYYIFSVRGVGGFNPFAIVKRLIKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XA62</accession>
    <name>A0A8J2XA62_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MMS19 nucleotide excision repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_01068g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the MET18/MMS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316463">
        <property type="protein sequence ID" value="CDF91161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XA62"/>
    <dbReference type="OrthoDB" id="342900at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039920">
        <property type="entry name" value="MMS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024687">
        <property type="entry name" value="MMS19_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029240">
        <property type="entry name" value="MMS19_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12891">
        <property type="entry name" value="DNA REPAIR/TRANSCRIPTION PROTEIN MET18/MMS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12891:SF0">
        <property type="entry name" value="MMS19 NUCLEOTIDE EXCISION REPAIR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12460">
        <property type="entry name" value="MMS19_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14500">
        <property type="entry name" value="MMS19_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="MMS19 N-terminal" evidence="7">
        <location>
            <begin position="51"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="MMS19 C-terminal" evidence="6">
        <location>
            <begin position="572"/>
            <end position="971"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF91161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1022" mass="115769" checksum="BAE2CAD476A7AE21" modified="2022-05-25" version="1">MPNEESVSYLETSIVSFVSNIDVDQSSSDKTACQLADSISHKSFKLLDLIVALRDHLTSEDLTSRRRALCCLSTVLSKLPNNTLLKNEVSVVLSFYSSKIDDNLLTKETLSGILSLTEMKYISITEIYSILKILSTKYNPSSHLAPTRYFTFKILDAIHEKYNSRMLVDHELTDAFVKAFLHVGNGEKDPRNLLLSFRLNEIISSNLDCADKFKEDLFDILFCYFPITFKPPKDDPYKISNTDLKIALRSAIASSPSFAEDAFGNLIDKLTASSPSVKNDTLLTIEACIKNFGGEACLKHWLPLWNALKFEIMHNTDVGYPDPLISLPNQPSESDNYQLSLTVIRSIGQTLLQFDEKAFDKFFSHILEEVKPNFVYEKDLKQTCCILGSIASVSDITFDKVVTASLPLFFKNSSDISKLKLEIMNLSFFFDAYIKVFGQTAPGNVVQVPKNALVRRKDDILMSLSKALTGSPKVEVTIRTLSIIQFTKLTKMQGFLKNEETALVVQYISEALLTDNNKNIYHAGLESLKSIGEIHEDIVFEIALTKMLELLPVDPAEQSQLNDGEPVEKETILKTLLDFTNSRHKLIKESIIGLSKKLDQVAPHENSEEYCFLLISTIHTLLSNNIEFIKEEDVKSIKKDIELPLFAAINNCPSIGNDDYNLILLSNVLFFLSLKSPRATHQEDLIKYNKYFLDDFQLLTRPSRLVVPFGKLLCGIDKKCNFQNAADIFRQTVIILSNAKTNMSPFEKLGYQELLAIFANKWISEETISEISDWDNKSMKNLEVLIWVAKGIVMKNSPLAVNFQQQFVSLLSDKEVGSLVAKLFEIFVIDIISLQKYKGITWNGSTKLLYKQKFFNDVFNRLVSAFKDTSEMQIKSNYLTALSLISKHISSNLIEPYMNEMLPLLLQSLDMPNGEVRISALNTLIDTSEKFHQLIAEHVQTLSRKLLKLVIPSKYNSFGVRVLSLRLLETISHVVPLNYSLALKEEIIRGLEPVLDDRKRIVRKQCVNTRQAYFELGQVPLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2SZF5</accession>
    <name>A0A8J2SZF5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_11892g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2SZF5"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000910">
        <property type="term" value="P:negative regulation of sterol import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035376">
        <property type="term" value="P:sterol import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13279">
        <property type="entry name" value="PH_Cla4_Ste20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06614">
        <property type="entry name" value="STKc_PAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000139">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000356">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000705">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SAMKA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PH" evidence="11">
        <location>
            <begin position="64"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="CRIB" evidence="13">
        <location>
            <begin position="190"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="580"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="255"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="258"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="273"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="307"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="329"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="377"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="461"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="493"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="513"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF87674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="873" mass="96011" checksum="7DA78263224767F9" modified="2022-05-25" version="1">MSLSAAANKISDNDFQNIGPAPRPPGTGGSGYNQTKPITKLLNQLDLNREGSGIKSLSQNSMNFKKRTGWISYKDDGLLSFIWQKRFLVLSDSYLALYKNDKVSEDPVLQIPLTSIVSVSRTLLKQNCFEIVRKSDRNSSNSLSSAGSGSSGGGLDPGSKKSIYIATKTEQELHSWLDAIFAKCPLLSGVSSPTNFTHKVHVGFDPETGSFVGMPSNWEKLLKHSRITGEDWNNDSAAVIQVLQFYQEYNGISDKNENGTNGAPTNLSQTSLNGNNTNNKIPNGNAGHNSNSPGSNSSSESLRTNSIKQQNSTNTNGLLPQRHPPTPPNSKGTTSAASTQSMKPNLNPLNTKTTSPPHAMNHQQHQMKMLQKRPMHSPTTGSFSQRSLPRNYPPQQQTFHPNHQFAQQQPHGPHEMSGFRPHHNMVNPYAKQPGSSMNNGPNALPRASPQSGYIDKNVNIPTRLRPQRAAPRPPVAAAAAAAAAAAATRQHSDSSQPPNMITNGGVANHTIAGANSPSPQKLPNLPQPQRQAQKKPDAEATNKDVGVTKQRKQAKSTLSNAEIMSKLKSVTIETDPSSYFNLIEKAGQGASGSVYLAKRLKLVPHTPEDTTSVDPPEVGDKVAIKQMILSKQPRKELIVNEIIVMKDSRHRNIVNFLEAYLKTEDDLWVVMEYMEGGSLTDIIENSAAAGSNHSPLTEPQIAYIVRETCQGLKFLHDKNIIHRDIKSDNVLLDTRARVKITDFGFCAKLTDKKSKRATMVGTPYWMAPEVVKQREYDEKVDVWSMGIMTIEMLEGEPPYLNEDPLKALYLIATNGTPKLKSPERLSLEIKRFLSVCLCVDVKYRASTEELLHHSFFSMACSHEDLATLLEWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T0R5</accession>
    <name>A0A8J2T0R5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_15808g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_15808g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T0R5"/>
    <dbReference type="OrthoDB" id="4069826at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000323">
        <property type="term" value="C:lytic vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035493">
        <property type="term" value="P:SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040939">
        <property type="entry name" value="Vps38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157">
        <property type="entry name" value="UV RADIATION RESISTANCE-ASSOCIATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157:SF5">
        <property type="entry name" value="UV RADIATION RESISTANCE-ASSOCIATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17649">
        <property type="entry name" value="VPS38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="209"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="425" mass="49335" checksum="060047C0CC52F7B7" modified="2022-05-25" version="1">MGPYLLKRRLRHVRSVKINNLCVTQKNSDDLFRFPSYFIVLENSRGETLYVSEVQNGSLYSVQFNELPLQHSPLTLFILKLFAEVPGKLSPYEDKVWCLFKNYKIDLNRMQYLKQDDIVGSYNAPLLELIDGNYVLPEVPIFSSSSSKTKEHKRKVSSLKNKPSFSFNYVLKLNKILEYTSQVMEESRQLSSKLELQIQNEHKKHIWVIDNLKHYNQQMKARIQKKRLELKKLDSMVALANTDLELPSEPHTNPLNDYYGNTYPSMVDSRNKLEALRTKKLAQLVSIFQMTELFHVRTGFATFDQSHQNASLYDKLSLQYLNKEELLKLATSGTKDVKKLTSTCLGYYTMFVTLIATQICSIPLPHVLWYCGSTSVINGTVPLYLDNNRDTTMLSQAIDYFNRDVVQIIQYLKHRRAQVVDNQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T1J1</accession>
    <name>A0A8J2T1J1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BN860_08064g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_08064g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T1J1"/>
    <dbReference type="OrthoDB" id="68020at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042791">
        <property type="term" value="P:5S class rRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16169">
        <property type="entry name" value="Tau138_eWH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046488">
        <property type="entry name" value="Sfc3/Tfc3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044210">
        <property type="entry name" value="Tfc3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035625">
        <property type="entry name" value="Tfc3-like_eWH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007309">
        <property type="entry name" value="TFIIIC_Bblock-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049543">
        <property type="entry name" value="WHD_TFC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15180">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15180:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04182">
        <property type="entry name" value="B-block_TFIIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20222">
        <property type="entry name" value="DUF6581"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21552">
        <property type="entry name" value="WHD_TFC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="B-block binding subunit of TFIIIC" evidence="7">
        <location>
            <begin position="109"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor tau 138 kDa subunit extended winged helix" evidence="9">
        <location>
            <begin position="547"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor tau subunit sfc3/Tfc3 C-terminal" evidence="8">
        <location>
            <begin position="738"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="477"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="674"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="478"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="509"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="674"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF87512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1160" mass="133051" checksum="AF63D5C41CB71DF2" modified="2022-05-25" version="1">MCQIPVYPDELVRLTCELIAYSGGEISLPLLWESSSKFVSLNDEMSKNFVFSCVTSDAAVTLYERGKVVHKSYSEIAANEDDFTLRIDEDTLWIILTGYRKKEANIGGSAFELLLEIAKAKESGINTKDLALATNQDPRSITGRVKKIGHLIFSVQMIYKGHVVKLLKLKKYEESDTTVKPYVNLRDHLEQIVDVVKKSKNGVRQIIDLKRELKFDSEKRVSKAFIAAIAWLDEKEYLKKVLVVSPTNPSVRIRCVKYLRDYVQEDKCINDFDYESDSGEDDIVDDEKLALDDDDAYEGLDSTNATSVLQQEGLVVQDEKSAEKNDILLNRFYPMQNQTYDITERAGLQGISTMEVISKMTGKDYKRAFTKSSEYYVDSVGKPTVQSSGYKIVRVYDFEGKKKFYRFFTEENFRKITDISEDITFNCFEPLPKQAENLLQLNGSNFVALSNTLHFMVRDGQEYFFWNGEVKFPSKISPAERKRKQEEKARNNKGTNTSLKRRKADRFLNTENETSSEGNILSNQAVEENGLSSKPKVLKVGGFSAGSLRSLRRQRAILEVVKKAGGVTYLREQFFEDVSQFMGSNTLLDKKTVRGDVNLMVENEKLRMQEEPNSGKRIIYLPGILSSTVSDFVIKEKDSRKAYFKDVIHNTDIYFFDQTEKHRFHRGVKSAERVRNYQNRDKAKKQPQDKATRKQAKNARALQKQYKLSRQLSSAAELATKNTVSRKAERSKQKTVFHVGNKVGARALIMAVVITKSIKNEIVWDQITKLFPNNSLENLKKQWTARRIRMGHAGWKAYVDKWRKVLLAATKEERVSLEDLERLDLPKLINLWIASDIEEDRKPIYLYRDYLENKRRYTLVRVSPTGGVKSGLAMSSMIQRESSSLKKVYVYDDEGCTRQHQLPAWGTEEDIRAVIRSILFDNSATSRDEIKVLNNVPKERVDKVIMDMAKEKHLYLHGSKLEATNTIKELLEAKGNYTMFEGSEIYRKKLEEMFRAGNGVIINEEIPDVASWIFIELITQSKVHLDVIPTMHNKNTLSYVTRRFGIRGLTPPLIISPNKPIIVEKEEQVPVPMGEPNSRLWIDSVGNLRPTVWKSLLSMLINEILFNPSICLGMLENNFSSALSKRELKDICDWLLKKGLIYETPFLGLKATHNWFTALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2F3</accession>
    <name>A0A8J2T2F3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_15302g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_15302g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2F3"/>
    <dbReference type="OrthoDB" id="539213at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF9">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="34"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="105"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="138"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="171"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF87824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="226" mass="25008" checksum="7C3453565420A268" modified="2022-05-25" version="1">MNLPLHEACMSNNLRKAQEIFNDSPQDLYQKDADGRIPLHWAVSFQSEEIVSFLLLKMVDVSVDNYKDESGWTPFHIACAVGNLYIVTNLYERHEKPDLNLPTNQGVTALHLAVSKKHTKICEFLLEKGASVRVKDKRSQLPLHRAAAIGSTSMVDLLCKAGSPINTSDAEGWTPLFHALAEGHGDVAVLLVQHGADPDLETSDGSKAVDFAVNDEVKKYFLRNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2H4</accession>
    <name>A0A8J2T2H4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">BN860_15654g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_15654g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2H4"/>
    <dbReference type="OrthoDB" id="5329385at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031342">
        <property type="entry name" value="Mug163-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17119">
        <property type="entry name" value="MMU163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF87840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="228" mass="25874" checksum="663C5A754BFCFA3D" modified="2022-05-25" version="1">MTSHLLQLAQLPQALRCLCRCISTRHSNLGKLTNYLVSQGVPNLLQTPLESKYIDDNIKLRLFPTTHPYIPVLQGRNKYRASMNAIRLLANKFVLRKGTRLRINAVSTILQEETSKYNCITTNDRLVVKWQSCAPDAGEEEMGKDHEIALVRDVRDIKASSPVINHIMNPATNKLTNEIESTQRPRFVRGIFIFEFNKDNSSILVHTIDSVETVDFEKKVQTNALASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2N0</accession>
    <name>A0A8J2T2N0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">U1 small nuclear ribonucleoprotein component SNU71</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_07558g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the U1 snRNP particle, which recognizes and binds the 5'-splice site of pre-mRNA. Together with other non-snRNP factors, U1 snRNP forms the spliceosomal commitment complex, that targets pre-mRNA to the splicing pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SNU71 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2N0"/>
    <dbReference type="OrthoDB" id="6275295at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.10">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002483">
        <property type="entry name" value="PWI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057543">
        <property type="entry name" value="SNU71_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057542">
        <property type="entry name" value="SNU71_RBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24826">
        <property type="entry name" value="SNU71_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24825">
        <property type="entry name" value="SNU71_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00311">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="PWI" evidence="15">
        <location>
            <begin position="523"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="305"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="452"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="305"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="344"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="453"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF87490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="595" mass="69263" checksum="DE2EFF60D267DA49" modified="2022-05-25" version="1">MNEIIYVSPQLYLVSDERWRSELEKPGYVPILRADIHKFQESLKSVLTARDLSSARHTSNLNALGQKDEHSAAESSDADNKIIQSAGGAAKYQSLKQFLPISLVQQLHTVSLMGFPTNISLHNLECFLCDCLGLVLERIHAAQDPDMLECWTNIQSLETQDIFIRIAERHKCFANFVQIMGDWFQSEEGPSVEIHIEANTAQFIKDQDVCNIELDKDLMRSLNQLWQQLNHSAPDKSLAPDNNVEYRIDLNTLSDLPQESLGQLCQDIMGFRTKVVTIEREKRVREAYEESKRRKQQMMKIFDQIRKSRRETGDHRPDEDDDEEEEEEEEEDDDDDDGEEDSVLEQHRVDQERRESENRYRTILKHLQTKIEPRLASLQERIYRASHYDQIRSDEQALYLKELLHQAVDPYYDHHRSYKLQEETADNIDRQEHPSYEAQPLEGAKLPTIGSEVQEHAQQKEKQQDLERSDVSYDDSQDQEQFKIKFAFKRAIDSSATHISSEDEDHDTAHAVAPEVLKEQDLDAKLKRLNESGLVQELVKEYLGVYEQDLVDYIYETIREKRSKHALLTELRETFDDDAVNIVEKIWSSEELNAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T347</accession>
    <name>A0A8J2T347_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BN860_15346g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_15346g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid/choline transporter (ACT) (TC 2.A.3.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T347"/>
    <dbReference type="OrthoDB" id="4476201at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000046">
        <property type="entry name" value="Amino-acid permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF6">
        <property type="entry name" value="GABA-SPECIFIC PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="156"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="201"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="311"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="366"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="415"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="441"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="484"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="516"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF87826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="577" mass="62588" checksum="031241FA97296F8A" modified="2022-05-25" version="1">MDIIEPQDIKESSSVTPRESVEKNATSVEFHNVGSHIRSVTSRRAGEVNYINAATSNSDNQLLAEIGYKQELQRKFSTLQVFGVAFSIMGLLPSIGTVLSTGLAGGPVSAVWGWLIAGCLILMVGLAMAENSSAIPTAGGLYYWTYYYAPVGYKEVLSFIIGCTNSLALTAACCSISYGFAEEVLSAVILSKDGDFTVTNGKLYGIFAAVVIMEGLCTCLASGVVAKLQSLSIYSNLFIIILLFIALPIGTKVNRGGFNDGAFIFAKYHNYSDWNDGWQFCLAGFMPAVWTIGGFDSCVHQSEEAKDAKKSVPLGIIGSIGACWILGWLIIIVLVACMDSNVENVMNTKYEMAIAQIIYDSLGKKWAIAFMSLIAFCQFLMGASTVTAISRQVWAFSRDNGLPLSKYIKKVNKKYSVPFVAIIAACCASLALGLLCLQGAAANALFSLAVAGNNLAWSMPTFLRLTYGRDLFKPGPFYLGKFMSAVNGWIGIAYQIFIIILVMFPSQQHNVTAPTMNYACVIGPGVWILSFIYYITYKKKYFHGPASNLTDDEYVDAVGEDMVENIDNILSNPEKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3N2</accession>
    <name>A0A8J2T3N2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA (guanine-N(7)-)-methyltransferase</fullName>
            <ecNumber evidence="11">2.1.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Transfer RNA methyltransferase 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA(m7G46)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TRM8</name>
        <name evidence="13" type="ORF">BN860_14576g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10 11">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms a complex with TRM82.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="11"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3N2"/>
    <dbReference type="OrthoDB" id="47276at2759"/>
    <dbReference type="UniPathway" id="UPA00989"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106143">
        <property type="term" value="C:tRNA (m7G46) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine(46)-N7)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000060">
        <property type="entry name" value="tRNA (guanine-N(7)-)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03055">
        <property type="entry name" value="tRNA_methyltr_TrmB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025763">
        <property type="entry name" value="Trm8_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00091">
        <property type="entry name" value="tRNA (guanosine(46)-N7)-methyltransferase TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417:SF16">
        <property type="entry name" value="TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 11" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="122"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="157"/>
            <end position="158"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="255"/>
            <end position="257"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF87791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="282" mass="32817" checksum="B05A83F7DFDD49C5" modified="2022-05-25" version="1">MKAKPLSQDPGSKRYAYRVSKEENRKELKHVKIDVAEDKKIDLPRKRFYRQRAHSNPFSDHQLEYPASPDEMDWAELYPEFQDQSTGEMTNQVTIADIGCGFGGLLVDLSPEFPNDLILGMEIRVQVTNYVEDRIIALRNNHAKDKGYQNISVLRGNAMKFLPNFFHKGQLSKMFFCFPDPHFKQRKHKARIITNTLLSEYAFVLKEGGVIYTITDVKDLHDWMVKHLEEHPLFQRLGAEWESKDRCVQIMTHATEEGKKVERKKGDKFVACFERLANPTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3N4</accession>
    <name>A0A8J2T3N4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_17194g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_17194g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3N4"/>
    <dbReference type="OrthoDB" id="10445500at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="211" mass="23479" checksum="6C8D58C3BEF267A1" modified="2022-05-25" version="1">MSPIFLNAIFNSTSICEPLSQSELSRVVVRPSLPVKELFIQRAAHKEYFSSNKLQSLVVILLMAAAARTLIYDAAKVEYKDIVTKILKSLKLRLTLLSKATLLLPIAVTLFRLEAQSLITSKQSSAHNPGEALDSKAGVGCDSDDDAETDPEIEQDTELEHQLASDTEQEVQQQYDLPATWRTQRKEVGLPYQDLTMSKSTALSLLLWDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3S3</accession>
    <name>A0A8J2T3S3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Restriction of telomere capping protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_04676g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat RTC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3S3"/>
    <dbReference type="OrthoDB" id="60955at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061700">
        <property type="term" value="C:GATOR2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16488">
        <property type="entry name" value="mRING-H2-C3H3C2_Mio-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037590">
        <property type="entry name" value="WDR24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049566">
        <property type="entry name" value="WDR59_RTC1-like_RING_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46200">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46200:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17120">
        <property type="entry name" value="zf-RING_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <keyword evidence="5 11" id="KW-0853">WD repeat</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="212"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="322"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="1220"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="671"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="718"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="827"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="873"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="920"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="678"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="718"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="831"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="873"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="920"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="943"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="983"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1000"/>
            <end position="1013"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF87362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1268" mass="140890" checksum="04FFCE9E071972EC" modified="2022-05-25" version="1">MSNRPIPRNQAGFSFGRNEMKPTSFGRSSFRIQHYSNSSQMSPVGSSPRTRMSPRYSFTGSVSFNESNLKESMFYDEKGKKEEVVPKSSGLSYSTVSSKELSSIDRINDPTLNSIICAGKTHLGLYKFSSEDNSIACVHDFMSSGYDTNRSASFHTGLAKRSKRTKLSTIADVKAGFHNHKNYVAVCGTSTSISIYDINKTNSMDNPVVTNLSQHTRSINSFDFNTAQSNLIISGGQDACIKIWDLRSSRSKTLNRSDVNINTASDSIRDVKWMPNQNFARMGSQSEMRSVGTAGYKFASIHDSGLLLTFDLRQPNQVEKRINAHSGPGLCLNWHPYQDYISTGGRDGKCCLWYVGENKPLSEFSPNGSTFTATPHSATPNLAANLLVLPETTINCGFPVTKLKVRPCYERKILNSMIAMSSMAEDFGVSIYSVARKYIPKYNLSTSSPSLGFVWWDENLIFNIDKEYKINGWYVNKEPTVLANMSKNITGWRDIEGNGLLFVDQDRGSYEVGDDMPINIEDTKKPANHRISMSSLNAVSSNSGVIGSIKKGISQTGLTSLSGERPTIGKTPANFSSKSMATQPMNLSHNNSVSSYPGLSLHNELPGCENVSSPFLVTLDLPYILNNMRISQLPPDKKFFNSPEVMAIKESPVKVFKFLAKELEFSYMQERRGGDVKSVTQQSNTNEDTTKKDLMEKFGLSENTTWTALISKKGEVAESESGKKDAPVKDFADSSAGSDAESADKGLHLDKVTSNVLSGDKPKLDKANELNIEVQERIDTLLELIPICDHNAAVYSYIDDLPNFKIWISIRDSLLWDLKGLSMDYPEGRASASQSTEDKQMGQQYSIGNDPPESVTSEIRSYITSDLNSFVEEQPKALRSDSDGQKDNKKPISNLQKQLTKIRESETVFEGTLANERRIVDAPEASHKYDTPKLPENRVSNDSESDVIEDEDEDKPGSPSQGIPILNKRKPRRSFIDTYMSGMKSSVGSNGESDTRLSKSRNSQSFGHSSPGSRMSPIASIGSAEFNPSNFKKIASRADRRSSGSLFMSPLKRKELFATEKSSKPSIERKSSIAPEQVSSLSLPPWNTRKLLKQIYKQAVEMGNTLLVVNILFLFQNLYQVTTTEVVKNSLAQFISILHRYELFELSAAILKYSPWEVVVNADGGQSLVPLFCERCGKLITNELSKERFTLEAHQEGNPLPLGKMGYWYCDSCKKPNTLCVLCERPMKTLTMCLLECGHEGHFECLKSWFLDEGMTECPGGCMNQVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3V6</accession>
    <name>A0A8J2T3V6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_18250g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_18250g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3V6"/>
    <dbReference type="OrthoDB" id="19806at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070822">
        <property type="term" value="C:Sin3-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038919">
        <property type="entry name" value="STB2/STB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059025">
        <property type="entry name" value="STB6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31011">
        <property type="entry name" value="PROTEIN STB2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31011:SF2">
        <property type="entry name" value="PROTEIN STB2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25995">
        <property type="entry name" value="STB6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STB6-like N-terminal" evidence="3">
        <location>
            <begin position="35"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="622"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF87954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="778" mass="89336" checksum="B60F29DB66F6F789" modified="2022-05-25" version="1">MTIEEGHKDNVFQLPRAGRTNKPINKTTVPEPLASYIFPDIRALHEIRLETFVHLTYEEITVHGFEIYIVEQWAAERKVSTVITSYTGNSQDTINAVQVVLPKNPERWPGKLREYYEDLSIFAKARSMPKGSLFITNLATVPSTLNLLHVECGDIRVIWKDFEVNFDLKKLRCGGRSALLLCGTSAAAEDKFRQLYKISIESSERERQLEELGQYEHFSQLKQNYNPVIELITMVQICLSYFNLYHDMKDGLLCDKTEAAVARFWQKYGKYYFGIDRPRNEGTSGPATVAALISLVLSCYFKLQVEDCISSKDPFEADEFFGGIYTFQKKYGLTRGTNPVYLDDLTLQRLFEVSAKTSNSDIFKFKKVVKSTVSDITGKGNPMHLSNEILTTDLDALVRKIHGGSLGLLWKSKGRLRKNFYKDYDNFCEIRYARGNPKAVLDKQANQMRSDGSESQESSSDEQGKHVFDTNLARYNTSSSSVSASSMFCNYDKANYASNFNINKLYHGEYFRRNSSPLLFNESSDQESLPPDEQSHGLYRANSFSDVQDVVEAWSLPFDPSLVKMARDLLKLHNQIQSQEREKKSNQGCMRGDEDLHAGHEGHYKVDFEQEDHCRADFEHLISHLQNNQQLYSHRVKELENNHKEVENKQLLLHGEMRELSSLASKFKYDMGILDIRMRDVEESISQFDKKLAAVQKSLVDQGLGTALGSELLVDKEQLEKCLQDFLQAENTKYEGVCCRVCSKKYFKQLHNDFKAWTSWTINRFFNRGEIPNTQKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3X9</accession>
    <name>A0A8J2T3X9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Coatomer subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_11628g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Oligomeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Peripheral membrane protein</topology>
            <orientation evidence="1 11">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasmic vesicle</location>
            <location evidence="11">COPI-coated vesicle membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the COPG family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3X9"/>
    <dbReference type="OrthoDB" id="1074925at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000368">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1480:FF:000001">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1480">
        <property type="entry name" value="Coatomer, gamma subunit, appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009028">
        <property type="entry name" value="Coatomer/calthrin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032154">
        <property type="entry name" value="Coatomer_g_Cpla"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017106">
        <property type="entry name" value="Coatomer_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013040">
        <property type="entry name" value="Coatomer_gsu_app_Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037067">
        <property type="entry name" value="Coatomer_gsu_app_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261:SF0">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16381">
        <property type="entry name" value="Coatomer_g_Cpla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08752">
        <property type="entry name" value="COP-gamma_platf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037093">
        <property type="entry name" value="Coatomer_gamma_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55711">
        <property type="entry name" value="Subdomain of clathrin and coatomer appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="13">
        <location>
            <begin position="20"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer gamma subunit appendage Ig-like subdomain" evidence="14">
        <location>
            <begin position="670"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer subunit gamma C-terminal" evidence="15">
        <location>
            <begin position="821"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="627"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF87662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="934" mass="104381" checksum="12DFF8C1E31B0BD5" modified="2022-05-25" version="1">MSTHTYKKFENAGSGDLPDRMTIYQDCMSEFNESPVNPKRCRLLISRLLRLLAEGETFPQNEATALFFSISKLFQHQNDSLRQAVYLAIKALSSISEDVLMATSSIMKDVQNGSDLVKPNAIRSLTSVLDESTAFSAERLFKSAVVSKNPSISSAALTSTYHLLPIAETTAKRFVNETQEAVMDLKPFPHKHTVGDYYPNSTFITQYHALGLLYQLKKTDKMALLKLIRQFSDNNTLKSQLAKVELVKLVTELVGRDPQLFSSFQPLLNNWLSNKYESVQLETAKLITAFAIQSSHLVSAELHASAVTTLQSLLTVPRVSARFAALRILNRVSMVAPEKIVVCNPELESLINDPNRNISTYAITTLLKTGTENNISSLISTITKFIHEVSDDFKIIIIDAIRTFSLNFPQEWKSILQFLIDVLKNGEGGLKFKNNIVEALIDMVSFVPQARDMALENLCDFIEDCEFNEILVRILHLLGKEGPSTATPSLYVRHIYNRVVLENSIIRSAAVVALSKFALTKNDPTLCESIVSLLRRIASDRDDEVRDRATIALQFIESARKEQSLEAREFVQNKYSYDLSSLETKLSAYISSNSDSFHTPFDVTNVRRYTEDETKAIELKRKQEQLLRGNLENGSESTPGLEEAGKAEKLDSSFAGPSLDEHQEDLLVTKYTDELLSIDQIKQFGPLVNSSKMIDLTDPEAEFVVRGVKHLFNNNAVFQFNITNTLTDIALDNVTVTCTPENPSESNLEELFSLSVDRLLPSQEAACYVAFKKSEDIVMEGFLNNLTFTTRELDPETQQPLDDTEGFQDEFDIDAIFLNAGDYIKSSFVGDFTSAFDELPNEEVAVYNIKENITLQEIVDRLVVNTSCLPLESTQYAPSDSSSHTVKMFGKSALKGDKIALVVKLIKTSKGIAMKAQGRSEDATLCSYLVNGLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3Z6</accession>
    <name>A0A8J2T3Z6_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SWR1-complex protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_07866g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SWC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3Z6"/>
    <dbReference type="OrthoDB" id="19740at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11658">
        <property type="entry name" value="SANT_DMAP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032563">
        <property type="entry name" value="DAMP1_SANT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027109">
        <property type="entry name" value="Swc4/Dmap1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12855:SF10">
        <property type="entry name" value="DNA METHYLTRANSFERASE 1-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12855">
        <property type="entry name" value="DNA METHYLTRANSFERASE 1-ASSOCIATED PROTEIN 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16282">
        <property type="entry name" value="SANT_DAMP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="10">
        <location>
            <begin position="159"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="14"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF87503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="474" mass="54739" checksum="4CA981CED36E4F9E" modified="2022-05-25" version="1">MSSSDIFDVLNIQQKSKSHGSSASPPPLSVPPTASKAPKPQVTGMQRELYNLLGENQPPIAIQPASKFKDKLTALSKPSPWTFAEFKANDAVRLHHWVKGSKELVSEETQESPYAKFNVHLRIPSFDKESYESFMSNDKSSNDSVKQEDSGSPDIVENGDWSYEEVEYLFQLCRKYDLRWFVIQDRYKYDKSRTLEELKERFYKVCQRFFLAKNPNDPLLPSLNFPKEKEIERKKYLQRLLSRSAAEIAEEEALIVESRKFEMAAKKTLNERESLLRLLDSPNSDQSVSQYLTSQGMSQLYGSLFSDKSRKRKHESNVPENPWMRQQQLFALQRQQLQQMHDRKQETQETGQLKKTKKQKQEMQTAIKRKAESVYAEHLLQNFNAEERRSLGVMAHGEKLPPGVYLRSTKISAFKPALQNKVATALQELGLSVRPAMPTYDVVQRHEELLKRIVILVTLKKHLDKLEAEKAITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T411</accession>
    <name>A0A8J2T411_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_11518g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_11518g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T411"/>
    <dbReference type="OrthoDB" id="10027013at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051052">
        <property type="entry name" value="Diverse_substrate_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942">
        <property type="entry name" value="METHYLTRANSF_11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942:SF4">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="3">
        <location>
            <begin position="40"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF88492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="294" mass="34317" checksum="D652B039AA395DB6" modified="2022-05-25" version="1">MSVFSAKDFNAQRYSTIRPTYPDSFYEVLSKYHQGPREMLIDVGCGPGTATFQLADCLNSFEKLIGTDLSSTMVENARATQEQNADKYANVSFVQSPAESFEFLGRNAYKQKCDMITAVECAHWFDFEKFQSAAAANLRAGGTLAIWGYGDFFFPDYPKLDFEIDDLTFGEDKFGPYWQQPGRNIACEMLKDLHYDSRWFSDAEEVYYYKEDLKGKGPKSTPLFMYKRLTLTDLKEYIKTWSGYHSWQKDHPKPEKDITDIFIERVKELYPELKDESEVQITWRTFWMFARRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T442</accession>
    <name>A0A8J2T442_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-5-taurinomethyluridine 2-sulfurtransferase</fullName>
            <ecNumber evidence="3">2.8.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_09692g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5-taurinomethyluridine(34) in tRNA + S-sulfanyl-L-cysteinyl-[protein] + AH2 + ATP = 5-taurinomethyl-2-thiouridine(34) in tRNA + L-cysteinyl-[protein] + A + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47040"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11726"/>
            <dbReference type="Rhea" id="RHEA-COMP:11732"/>
            <dbReference type="Rhea" id="RHEA-COMP:11733"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:87171"/>
            <dbReference type="ChEBI" id="CHEBI:87172"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MnmA/TRMU family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.14" evidence="3"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T442"/>
    <dbReference type="OrthoDB" id="3685at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01998">
        <property type="entry name" value="MnmA_TRMU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.280:FF:000001">
        <property type="entry name" value="tRNA-specific 2-thiouridylase MnmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000115">
        <property type="entry name" value="tRNA-specific 2-thiouridylase MnmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.280">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004506">
        <property type="entry name" value="MnmA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046885">
        <property type="entry name" value="MnmA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046884">
        <property type="entry name" value="MnmA-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023382">
        <property type="entry name" value="MnmA-like_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001138">
        <property type="entry name" value="PRK00143.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00420">
        <property type="entry name" value="trmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11933:SF5">
        <property type="entry name" value="MITOCHONDRIAL TRNA-SPECIFIC 2-THIOURIDYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11933">
        <property type="entry name" value="TRNA 5-METHYLAMINOMETHYL-2-THIOURIDYLATE -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03054">
        <property type="entry name" value="tRNA_Me_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20258">
        <property type="entry name" value="tRNA_Me_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20259">
        <property type="entry name" value="tRNA_Me_trans_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA-specific 2-thiouridylase MnmA-like central" evidence="13">
        <location>
            <begin position="239"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-specific 2-thiouridylase MnmA-like C-terminal" evidence="12">
        <location>
            <begin position="344"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF87580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="406" mass="46314" checksum="82F075E9C82DCD88" modified="2022-05-25" version="1">MLSRFNEIIGKRVVKGYKSQHFPALHDNVIIAMSSGVDSSLSAALFAGHFPKARGVYIQSWGKSQSLTDPRLEPCYERDWKDVCRVAQHLNIPVDKMNFEKDYWLDVFEPMLENYNQGMTPNPDIGCNKFVKFGKLRQVLDQTYGARNYWLVTGHYSRILNSLNDDKPHLLRSFYTCKDQSYYLSQINADYLSQLIFPIGHLTKPEVRQLAAVAGLSTATKPDSQGICFVNNSQHGKFKHFLRHYLPDSVGNIITVDENEQKRIWGKHTGLWSYTIGQKVGISMPQGDPRYAGAWYVSEKLHDSNEIVIVKGRDNPALYKDVLYVSNIVPMDRNFRSLLEEGISNQNLFVQYRSLQEPVPLISCDFSHQLVIKLARPQRAIAPGQYCCFYLNNRVMGSGTISRVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T450</accession>
    <name>A0A8J2T450_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glucosidase 2 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_14202g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T450"/>
    <dbReference type="OrthoDB" id="28322at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017177">
        <property type="term" value="C:glucosidase II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039794">
        <property type="entry name" value="Gtb1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036607">
        <property type="entry name" value="PRKCSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028146">
        <property type="entry name" value="PRKCSH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630:SF1">
        <property type="entry name" value="GLUCOSIDASE 2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630">
        <property type="entry name" value="N-LINKED OLIGOSACCHARIDE PROCESSING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12999">
        <property type="entry name" value="PRKCSH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13015">
        <property type="entry name" value="PRKCSH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035182518" description="Glucosidase 2 subunit beta" evidence="6">
        <location>
            <begin position="24"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="7">
        <location>
            <begin position="497"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="445"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="653" mass="74372" checksum="50FDE1233BA12F2F" modified="2022-05-25" version="1">MATRLNSVLLGAIVGLCAFRVLAQNSETVLGVPEVNQPLYVAKDGKWACLSDSSIVIDSSQINDGICDCPDGSDEPGTGACGMSGPQFFCKNKGFLPRYISQSKVGDGVCDCCDCSDEELTTGHVFYRGANCSQLQLSFNKFVETELRRYEAGSRALKKMTAHADSSQKPKSKEELSQEISSLSEKLANSEKVLEGEKAEYLERLKTDEPLLYELEQLHIDKLARDVNEQFESVIKVSKAYEELVEILDGLVEGYSRHLQDLVVNDNVKKYQFYKQQNLLKLTCDSKNENGLRDQLMVYFEKELPELFSHGLSDKPAKYLQGKATFVDMLILSKVEYTEVVMEAVGKLREMMLDISENYNRNYQDQDVKKAVEAFKRYTSKYLSVGVVQMPQLLAEKLEYLREFVSAKAPELVTLAGSDIGAEEGGNGLMKQFHYLAQEIPRFFKPDIKSQIEKHQQDVESLRSQVTERRKDLNDIIKRDELGDDERTKSIKKLINDMCPYVENALDGYTYEIHFNGHIFQRANFGDQNQVSIGSFQNLRLNQDLALHKYQEYVRIKYSGDDELVQHLISETKDDKEEYLFGNLYQLNNGLELEFDHGDKCWDGPERSASVFVQCSEKMQINKITEVSRCRYVIELDSPYGCSQVFTYQPFTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T488</accession>
    <name>A0A8J2T488_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BN860_11870g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_11870g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="294853at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032817">
        <property type="entry name" value="Mon2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16206">
        <property type="entry name" value="Mon2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mon2/Sec7/BIG1-like dimerisation and cyclophilin-binding" evidence="5">
        <location>
            <begin position="8"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2/Sec7/BIG1-like HUS" evidence="3">
        <location>
            <begin position="208"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2 C-terminal" evidence="4">
        <location>
            <begin position="914"/>
            <end position="1053"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF87673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1617" mass="184441" checksum="DE3A336DAF2DE992" modified="2022-05-25" version="1">MAAIGGEFSSFWKQVNAELHALSSESKKRNSGIKNASDKSIEILKVVHSYEELSRHPDFVAPLVMSCSSKNAKLTTISMQCFQKLSAVPCIPVDKLSDVLDAFIEANQLAMDIKLKVLQILPIFFKVYGKNVYGKLCMKLLKCCSDLLQLPNKSSMVLGTASATLQQLIDEIFERLSLQQKDDDNLIKDENNHEVLIGNNEFIKVNVYRADANRLFSDLCSSFAPAEQPSKDVLLDVKNLPVNYGLEILESILSNSKCVFLEYQDLQFLLRIKAVPLLLRFISSSKNFSTVVRSYRCIRLLIIREYLEVLELELEVILSLLTHNISHDSDAPLWKKALSFEIFMELSKDYNLVCGIYMTYDNFADKKHIVSNFLRDSLQLLQTEEYSQYLCESSIVEKMDMPLISTETSALKTHYLHMLDKSTPPPVNATHLIWLMLFISNELSESLSSAALHCSQSVQEEEKGSVKNVLNGMFPGLFAMHGKFLYSTSLDNALFHSLIRAFQKLAHASGILCMNQQLNQCLELFSSAIVKNVDLSREKSKIDPQQGSTVLNTLSETLIGSTTSEKLTESEVERKILPVRVLNQRHVSLFRALISLSMSLGASLEIESWSSILRTWQWVAYYIYGPSADFMENFYLQDVPPPPAMTKSEVISIENSIGNLFESTTSYSNPAFRTLLSALISESKRTLSLAADVNNGEINLHYDEQKSVSVCIYNKIFYIAQIGELAIYNCSRFVNDQKDRKNWTTIMDYLTSLISDRKLESVAFRLYANKILTDIIRKTSIEVANNEDQEARNKSFNILETLVMDSLMGVIDAFKNLEVGKEEIYHGVANAESEILFELLTTLKDLLNEFGDLLGHSWSTVFKIINAPFEWGQIDANPVFMALQDNSSLVNGIIQKRSEMIQVSYEVFKLISDDFLQALPLDVIKGVIDTLMNFVGQELNLNISFSSISQFWLVGDYLRVRKGEEDRGMEAEFIKDIEEGKLEETITSKNSPYYKIYNGLWLYLLQQLVECSKDKRIEVENGAVQTFFRIIDSHSAYFPNWDLLFLVVVKPLLTDHCYNENLAKGSDFWSHTLPGLVKLYRSNFSNFTEHSSAVDEWLILLDALQHFTSSDATELAYVAIQNYRNLLEFAVGIDCFPAIVLNKCIEIWCGYHIVYGDITGSNEYSSKTGYDCIEELVTGFPFLYQIMMKHDRITVEFMEKTLSLFYSAVRYPLLPEHTRDNRKPSSLQLAVLRGLNTFKTTQKSELEVLVLLQLSVMVTLPLDTREKIEKKLLPKLSKSQISRIPTFEAICYDSFEELNRRLHVRADLDFSSASNKKIMLRVLKNLGEMASRKSLIDLSPQKDNPIWILSSQCFRDLFLRMFDSFTLTNVSEKKKESFFSVFINVAISSLQKHGDYTDAKTEVADLVEYGNFRDIMLREDVIKFIEDSKIEHFISAVWNCSFLFEMDEVEKEMLKHSKSLLQVAENLSTFDFSDISGCTVENPLFTKHNCALACLEDLLRFIQIPGDNFARLRRLSAPFLVSRVAFVLRRYVSNESLVRRAPIPKVRKMELEILLPGLHHILDLFLTDNRSEEHSVIDALKLLHPLILRAIPLSHKMNVLQYEVMELSLGFTKLSPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4C3</accession>
    <name>A0A8J2T4C3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA topoisomerase</fullName>
            <ecNumber evidence="6">5.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_13762g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="6"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4C3"/>
    <dbReference type="OrthoDB" id="430051at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035825">
        <property type="term" value="P:homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03362">
        <property type="entry name" value="TOPRIM_TopoIA_TopoIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.290.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.140:FF:000003">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="entry name" value="Topoisomerase I, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034144">
        <property type="entry name" value="TOPRIM_TopoIII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390:SF21">
        <property type="entry name" value="DNA TOPOISOMERASE 3-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PROKARYOTIC DNA TOPOISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00396">
        <property type="entry name" value="TOPO_IA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="7">
        <location>
            <begin position="2"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="8">
        <location>
            <begin position="172"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF87757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="646" mass="72960" checksum="C5A405C6404B82D6" modified="2022-05-25" version="1">MKVLCVAEKNSIAKAVAQTLGGGSLSSRDSGYMYVKNYDFSFDGFPFAQGSGSCHVTMTSVAGHLTGIDFQNERYGWGKCRIEELFDAPINEVLDKNQRKIADNIKREARGADFLMIWTDCDREGEYIGWEISREASRNNPRLTSEQMYRAVFSHLERRHILDAAKRPQRLDMRSVSAVGTRMEIDLRAGVTFTRLLTETMRNKVGGAGVASNGNRKKDTSTVISYGTCQFPTLGFVVDRFERIRNFISEEFWYIQLDIKSSENETTAFLWDRGHLFDRLAVLTFYETCIELAGNVAKIVDVRSRPTSKYRPLPLTTVELQKNCARFLKLNAKQSLDAAEKLYQKGFISYPRTETDIFPKTMNLKALIEKQAQLGQDGRTAWAAYAEQLISSTPGNNKFKTPRSGTHDDKAHPPIHPIVSVGPQANLPKVERQVYEYVARHFLACCSEDAKGHSTTLILDWGGEKFSASGLVVLERNFLEVYPWAKWENTKRLPELEVGQVCEVSKTKMRSGSTSPPKPMTESELIMLMDANGIGTDATIAEHIEKIQTRNYVRGEKAGKETYLQPTILGVALVHGFEAIGLEDSFAKPFQRREMEMELKQICEGLRTKADVVDDIVSKYRRYWAKTNGSKNTLLQVYDRVKASMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4D5</accession>
    <name>A0A8J2T4D5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BN860_13718g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_13718g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4D5"/>
    <dbReference type="OrthoDB" id="2018619at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903089">
        <property type="term" value="F:5-amino-1-ribofuranosylimidazole-4-carboxamide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015888">
        <property type="term" value="P:thiamine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11482">
        <property type="entry name" value="SLC-NCS1sbd_NRT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4160.10:FF:000005">
        <property type="entry name" value="Thiamine transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012681">
        <property type="entry name" value="NCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00800">
        <property type="entry name" value="ncs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF15">
        <property type="entry name" value="NICOTINAMIDE RIBOSIDE TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="236"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="337"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="434"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="505"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="546"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="628"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="645"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF87755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="657" mass="73546" checksum="7013CC4D28EEABED" modified="2022-05-25" version="1">MSIKSNNQYGVQEVDESSLKELEPQPPTVEPSSAISEHSSTVEETPQLRRRLPFVSPLGSLGSSLGKTSRKCLKWLEVPEDERSTLSFLRNPDLLPIKKENQTWGFWSNFGYWGVMSFSVGTWMSAVSAADYGLSYPATIGTFIVGDVLTIFFTLANSYPGLDWKVGYTLSQRFTFGIYGSIVGVVIRILMSIVNYASNAWLGGLCVNMILDSWSHHYMHLPNTLSPHIAMTTKEVIGFILFQVITCFFYLMKPYHLNYLLIWSCAACCAAMVGMVIYLTVKAHGVGDLFTSLESTAKGSDYAWAWVYMISYWFGSVSPGSVNQSDYSRFASSKTAVYLGTIMALLIPTTIVPIFGVIGASTCEKLYGQPFWYPMDIFNYWLTQDYSAGGRAASFFCGVAFVTSQIAYTAENAGFASGMDLAGLVPKYINIKRGALICAAISFALQPWNFYNSTSTFLTVMSSFGVVMTPIIAVMIADNFVIRKRKYSTTQAFIVKGEYYYCWGVNWRAMFAWIAGMAPGLPGIAWEVNNKYFNNVGIVNFYYGDSFFSFVISFFLYWILCLVFPVKINIKRDDRDYYGAFTDDEARAKGMIPYSEVSLEEIAMHQNNIHPSPSAPASCEITAHDEIHSKTEKDLQEQPESPYSEWDKTSIADRDNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4H3</accession>
    <name>A0A8J2T4H3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_15830g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_15830g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4H3"/>
    <dbReference type="OrthoDB" id="783096at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07383">
        <property type="entry name" value="MPP_Dcr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32440:SF0">
        <property type="entry name" value="PHOSPHATASE DCR2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32440">
        <property type="entry name" value="PHOSPHATASE DCR2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="243"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="577" mass="66752" checksum="4066CA69E96F5B5C" modified="2022-05-25" version="1">MPILSKRYLRYLPYILLFVFLEKSVIFGPWHQSSFPFKRKADHIRSHSSLRAWLRANNLEYVDEYDHVVVNVGRMQCFHLGDWWSHCAVKDELKPQNSAEVQRKIIRKDLLGAKTFGFSDYLFYDTLSIYSISQLEDNDQKEQVLLTAVSEQQSPDSQKVYNFYVNFVPLQVEYLTNGTPIISDFNVLFGDDCVDPRNNWELDKSWILYRGQFPSYLSIRRLSAKHIEDWHPRLTVREDGTYKIVQLADLHFSVGKGECRDEFPKHDFCEADPKTIKFVEEVLDLEEPDMVVFTGDQIMGDRAVQDSETALLKAVAPVIKRRIPWAMVWGNHDDEGSLSRWSLSKFANGLPYSLFTISPRDTKDNTFGVGNYYHQILDYEKKYPVLTFYFLDSHKYSTTGKIFPGYDWIKEAQWDYLEKIYDEELYHHIKEIPAYHLSMAFFHIPLPEYLNEASKRNPGESNPIIGNAKEGVTAPKYHSNGVDSLVHMNVQATSCGHDHCNDYCLLDDSSTSKLWLCYGGAVGEGGYGGYGGYERRIRMYQFQTKQGNIYTWKRLNGSPINYFDYQPLALGGVSSTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4I8</accession>
    <name>A0A8J2T4I8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial pyruvate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_16644g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the uptake of pyruvate into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4I8"/>
    <dbReference type="OrthoDB" id="1697690at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006850">
        <property type="term" value="P:pyruvate import into mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005336">
        <property type="entry name" value="MPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03650">
        <property type="entry name" value="MPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF87883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="127" mass="14543" checksum="221C09EEB97FA05A" modified="2022-05-25" version="1">MSQPFQRAAQRSFISKYINKETLKYIFTTHFWGPVSNFGIPIAALYDLQKDPTLISGSMTTALLVYSAVFMRYALAVKPKNYLLFACHVINETAQLGQAYRFLNFNYLSSPEEQEEIRTKYAAVVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4M5</accession>
    <name>A0A8J2T4M5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">BN860_17986g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4M5"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098588">
        <property type="term" value="C:bounding membrane of organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16489">
        <property type="entry name" value="mRING-CH-C4HC2H_ZNRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051878">
        <property type="entry name" value="ZNRF_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46661">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZNRF1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46661:SF4">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15" id="KW-0458">Lysosome</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="20">
        <location>
            <begin position="17"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="225"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="104"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="137"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CDF87943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="283" mass="32318" checksum="6B49094D1F2CA08E" modified="2022-05-25" version="1">MLNGTNYVNSFAQWQPDENMRNCLNCHAAFSFIVRKHHCRCCGGIFCGSCSEKFARYDNQRVRVIKRSATDEEFPPFRTCDSCYDNLLHMKLLFSPWGKRLEASGPPQAVEGNDFRSDSPTPSYTPGEVVKNTGNGVKDDSQGPSEDVRSVEPNEENSCPICNAFLGELESETQAQQHVEECIQKAEMTQQHHELRNEIGSTTFQNRMLVYKIPEASIEEEFPECPICFEEMVPGDKVGRLECLCVFHYKCIKSWFRKKAQKIKSNDVKYIGKNFCPLHDAVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4P6</accession>
    <name>A0A8J2T4P6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_18778g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_18778g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4P6"/>
    <dbReference type="OrthoDB" id="440781at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16105">
        <property type="entry name" value="Ubl_ASPSCR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021569">
        <property type="entry name" value="TUG-UBL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46467">
        <property type="entry name" value="TETHER CONTAINING UBX DOMAIN FOR GLUT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46467:SF1">
        <property type="entry name" value="TETHER CONTAINING UBX DOMAIN FOR GLUT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11470">
        <property type="entry name" value="TUG-UBL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="265"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="413" mass="46566" checksum="4D94F55E0724D3EA" modified="2022-05-25" version="1">MATVVICYNFNTFKVKVTPSTVLNDVLSQSLQHFGLSQDNGGKWSLIHGDIPVELDLPWRLMNLPTGIKLELEQLDSKKSNESKLIKIRVQAPGYNNSVVKQVDVTQNLKQLLQELGKENDWPIQDPEAKLQLFTKTIPLAELTNQTLRSLGVLESVSIRLVLPCTASNDARVNTSQSAEKPFEGKKSEHPETPKKIHQLHQVSAFVPPQNTLASQLREEEVEAHELTVEHARKYQQMVLKQTGGLGGPMLPRRLREQTEAAKKKEIQECVVRIRFPDRNHVEVAFEPEDTMQTVYKVVAGSLVNEELEFTLFQSHPYIALPSDGRKLASDWQFGAKTLLLFDSKKKGPYLKDSVLENARNLTDASEVKLDRSEEARPNHESGDKGKSKMQSTASSRSMTPGKYPKWLKLNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4Q1</accession>
    <name>A0A8J2T4Q1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_19108g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_19108g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4Q1"/>
    <dbReference type="OrthoDB" id="2253354at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="225" mass="25853" checksum="46494DE52DEC5141" modified="2022-05-25" version="1">MLGRVVRPLSVFRLYSQIAPRPGKSSRFDRTIIKPLVVVIVFGTMLTHVTNQQRLAGEMERRYFLKIEILKDLIERAREGDTNINVQEELKLVNKLFERTSEARELNAREAEKLQEISSSQNYSEKEVLQSLNRSPRALEKESLDDLLKSIMDEISDEPKQPIPRKSSPTPNSNEVILDKDVLKEEAKYERELSQYKPSTDVHLIVESPGELSSAAEDTKVTRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4R5</accession>
    <name>A0A8J2T4R5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP-dependent RNA helicase DBP3</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ATP-dependent RNA helicase dbp3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">BN860_16754g</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">ATP-dependent RNA helicase required for 60S ribosomal subunit synthesis. Involved in efficient pre-rRNA processing, predominantly at site A3, which is necessary for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4R5"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002174">
        <property type="entry name" value="ATP-dependent RNA helicase DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 17" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="21">
        <location>
            <begin position="133"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="162"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="348"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="133"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="59"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CDF87888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="543" mass="61654" checksum="8C4591988122865C" modified="2022-05-25" version="1">MSRKEHKAKKRANEDEDDIQFKKHKSNGKEQKVRDKKEHKKKKEHKDKKGKNNKKEKKVKRDNSETTDGEQIKSDVEEKTEKSAGNESIKDAPGGYRQSEDLSNIPQADVDIFYKENEVSVEDKLNYNYRPLLSFNHLSLKPEIQSEISKFPKPTPIQAVSWPYLLAGRDVIGVAETGSGKTFAFGVPAINYLVDNRSQKRGVQVLVISPTRELASQIYDNLILLTDKVGLECCCVYGGVPKYEQREQLKKSQVVVATPGRLLDLINENSVDLSQVRYLVLDEADRMLEKGFEEDIKSIVKHTNTTDRQTLMFTATWPKEVRELASTFMRDPVKVSIGDRDELSANKRITQIVEVIEQRQKDRKLLDLLRKYQSGSKKDDKVLIFALYKKEASRVENNLRYHGYDVAAIHGDLSQQQRTQALNDFKMGKSNLLLATDVAARGLDIPNVKTVINLTFPLTVEDYVHRIGRTGRAGQTGTAHTLFTDQEKHLAGALVNVLNGANQPVPEELKKFGTHTKKKEHSAYGAFFKDVDLTKKPKKITFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5B3</accession>
    <name>A0A8J2T5B3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 18">Pyruvate carboxylase</fullName>
            <ecNumber evidence="7 18">6.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">BN860_13168g</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.</text>
    </comment>
    <comment type="function">
        <text evidence="3">Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20844"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 18">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.4.1.1" evidence="7 18"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5B3"/>
    <dbReference type="OrthoDB" id="196847at2759"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07937">
        <property type="entry name" value="DRE_TIM_PC_TC_5S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000018">
        <property type="entry name" value="Biotin carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000010">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000600">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.600.10:FF:000007">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000033">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.470.20:FF:000012">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.600.10">
        <property type="entry name" value="pyruvate carboxylase f1077a mutant domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003379">
        <property type="entry name" value="Carboxylase_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055268">
        <property type="entry name" value="PCB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005930">
        <property type="entry name" value="Pyruv_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006761">
        <property type="entry name" value="PRK09282.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009554">
        <property type="entry name" value="PRK12999.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01235">
        <property type="entry name" value="pyruv_carbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778">
        <property type="entry name" value="PYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778:SF2">
        <property type="entry name" value="PYRUVATE CARBOXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02436">
        <property type="entry name" value="PYC_OADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001594">
        <property type="entry name" value="Pyruv_carbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89000">
        <property type="entry name" value="post-HMGL domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14 18 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15 18" id="KW-0092">Biotin</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="10 18" id="KW-0436">Ligase</keyword>
    <keyword evidence="11 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12 18 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="25">
        <location>
            <begin position="18"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="24">
        <location>
            <begin position="140"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="26">
        <location>
            <begin position="557"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="23">
        <location>
            <begin position="1094"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="312"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="566"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="638"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="21">
        <location>
            <position position="734"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="764"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="766"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="898"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="22">
        <location>
            <position position="734"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-biotinyllysine" evidence="22">
        <location>
            <position position="1135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR001594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="CDF88563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1177" mass="129451" checksum="496FC85D286E5D6A" modified="2022-05-25" version="1">MSTKKFSGLRDNFSLLGERNKILVANRGEIPIRIFRSAHELSMRSVAVYSHEDRLSMHRLKADEAYVIGEEGKYTPVGAYLAIDEIISIAKKHGVDFIHPGYGFLSENSEFADKVAKSGITWIGPPADVIDAVGDKVSARNLAAKADVATVPGTPGPIESAEEALEFTKKYGFPVIIKAAFGGGGRGMRVVREGDDVADAFQRASSEARTAFGNGTCFMERFLVKPKHIEVQILADNYGNVVHLFERDCSVQRRHQKVVELAPAKTLPIEVRNAILTDAVKLAKVAGYRNAGTAEFLVDNQNRHYFIEINPRIQVEHTITEEITGIDIVAAQIQIAAGASLEQLGLLQDRITTRGFAIQCRITTEDPSKNFQPDTGRIDVYRSSGGNGVRLDGGNTYAGAVVSPHYDSMLVKCSCSGSTYEIVRRKMIRALVEFRIRGVKTNIPFLLTLLTHPTFISGEFWTTFIDDTPQLFQMISSQNRAQKLLHYLADLAVNGSSIKGQIGVPKLKKHPDVPHLHNSSGEIIDVVSTPPPSGWRQVLLEHGPDEFAKRVRKFNGCLIMDTTWRDAHQSLLATRVRTYDLAAIAPTTAHALAGAFSLECWGGATFDVAMRFLHEDPWERLRILRKLVPNIPFQMLLRGANGVAYSSLPDNAIDHFVKQAKENGVDIFRVFDSLNDLEQLKVGVDAVKKAGGVVEATVCYSGDMLQPGKKYDLDYYLEVTEKIVKMGTHFLGIKDMAGTMKPAAAKLLVGSIRAKYPDLPIHIHTHDSAGTGVASMAACAVAGADVVDVAVNSMSGLTSQPCMNALLASLEGEIDTGVNATHARELDAYWAEMRLLYSCFEADLKGPDPEVYEHEIPGGQLTNLLFQAQQLGLGEKWAETKRAYRQANYLLGDLVKVTPTSKVVGDLAQFMVSNKLTPDDVKRLASSLDFPDSVMDFFEGLIGQPYGGFPEPLRTDVLRNKRRKLTCRPGLELAPFDLEAVREDLQDKFGDTDECDVASYNMYPKVYEDFQKIKEKYGDLSVLPTKNFLSPPEIGEEIEVTIEQGKTLIIKHQAVGDLNKETGLREVYFELNGELRKIHVVDRSQKVEIKSKPKSDGHDPLQIGAPMAGVIVEVKVHKGSLVKKGQPVAVLSAMKMEMVISAQTDGQVKEVFVSDGENVDASDLIVTLEDVAPAKET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5F4</accession>
    <name>A0A8J2T5F4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_07404g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_07404g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5F4"/>
    <dbReference type="OrthoDB" id="2430277at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22064">
        <property type="entry name" value="WH2_WAS_WASL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WH2"/>
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            <begin position="30"/>
            <end position="47"/>
        </location>
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            <begin position="1"/>
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        <source>
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            <dbReference type="Proteomes" id="UP000019375"/>
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    </evidence>
    <sequence length="779" mass="77121" checksum="2EBC6A801A78E9D4" modified="2022-05-25" version="1">MPGPPPPPPPPPGFGAGPAAPKPGASVMQGRDALLGDIRKGARLKKAETNDRSAPQVSGGGVTGSSSSGSGSSHSVSSAPPIPIGGGPQLGDILAGGISKLKPINKGPSPPSAPAAGAPPIPKIHSDLSSPPRLPAAPPTRPRPSMAAPQIPSAPAPSIPSARPKRSSMLKKSGSSGPPRSPASPAPPIPMGAPPPPVASAPPIPSAPSTPAPPPPPTPPPVAMAHTSSSSSDASAHAAPAPGGLPFLAEINARRSDRGVTEGISKFPSASGLSPRSSSVPPAPPIASAPPLPSAAPPVPHASAPKLPTTRPSQKSGAPRIPSGRPSRLKSVKKSESTGLPAVAPPAPPAPPAPPAPPAPPAPPAPQALSAPPLPSASAPQSPSPPAAPPAPPAPPASPSAPLAPPPPSLGGRHAGKTRKKSSGKESTPVSGGALPFLAEIQKKRDDRFVVDGKSGYSTKAEQEDAAAEDVAAPAPSSMHHTESAGAGMSFLGEIQSKLKHQHHTGSPSAPTAAAPPLPAASAPPVPPMAPSKTSASHRSVSSRIPSAISPSTPQIPSAPSPSIPSAPSPFASSPTPKDSADNGSSFLNDIASSIRHKRQGSSMSASLSVPAAPSPPPAPLAPPSPPPTSAPPLPNASAPKVPASPPPPPMSTSSSFLEQASMKPPPPPPQTSKSSATSSSGDAGTNFKQRLFSSGSSVLKRNNNEHTNSPDVDVGAVTINGSRSASGKVKSGKTYINDSRFRWMNASQIPKPPMFQGKTKLYPSGKGSSVPLDLSQFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5G2</accession>
    <name>A0A8J2T5G2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Chromatin-remodeling ATPase INO80</fullName>
            <ecNumber evidence="15">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">BN860_05666g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">ATPase component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the INO80 chromatin-remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The DBINO region is involved in binding to DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="15"/>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="372624at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:transcription by RNA polymerase II"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="DEXQc_INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SF2_C_SNF"/>
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    </dbReference>
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        <property type="entry name" value="Blast:Putative DNA helicase Ino80"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SNF2 family helicase/ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR031047">
        <property type="entry name" value="DEXQc_INO80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF2">
        <property type="entry name" value="CHROMATIN-REMODELING ATPASE INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13892">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51413">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DBINO" evidence="20">
        <location>
            <begin position="442"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="681"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="1247"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="122"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="173"/>
            <end position="292"/>
        </location>
    </feature>
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        <location>
            <begin position="370"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1391"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="513"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
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        <location>
            <begin position="130"/>
            <end position="143"/>
        </location>
    </feature>
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        <location>
            <begin position="208"/>
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        </location>
    </feature>
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        <location>
            <begin position="271"/>
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        </location>
    </feature>
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        <location>
            <begin position="405"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1393"/>
            <end position="1404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CDF89497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1411" mass="163104" checksum="B87C3A69C2F82A4A" modified="2022-05-25" version="1">MSLAALLNREDEQHEEKQRPPEPVALSNGHGGGEPVQKFLETLNQEYAVISERDVLEHEYQDWKFINYQEFELVSEWNNQSKDWKGEVFDALYREMEEMRGEWEAYALYKENKLKLLRELDKSEEEERNKIKRPKRAPRKRKIHELEEGDAQQQQPLLAADNHVRVEELDTKVAYQPQQQVNGKHEEVPEEIEAVHPEQQQQQALDGKNQELNHDGERDSGSDHEHELLKTEHEPEQVPEQEAEQGPTRDPEQEEDSHDAPFALEDDENDKDFSLEDEKPRPSSKKDKTVTLESEKPKIVKELIKMCNKNRVVKVKKRRFTNAIVTEYNPLDNKLSVKVTLKQYHVKKLKRQINEAKRAAAMEEAAKAKEEAARNKEISGSPSGKKRKVIGKDGSVDGTPGNEVDSATASPTKNGLPTYGLKMTVKEARAIQRHYDNTFFTIWKDMARKDCAKMARLVQQIQSIRTANFKKTSSLCAREARKWQFRNFKQVKDFQTRARRGIREMSSFWKKNEREERDLKKKAEKEAMELARKEDEVRESKRQAKKLNFLLTQTELYSHFIGRKIKTNELEGAMADQNIQQQGELGSHVDLDTTEANKTDFHNIDFDNEDDEQLRIKAAQNASNALAETRAKTKEFDDSSANGEELNFQNPTSLGEITIEQPHMLACTLKEYQLKGLNWLANLYDQGINGILADEMGLGKTVQSISVLAHLAEKYNIWGPFLVVTPASTLHNWVNEIAKFVPEFNILPYWGNANDRKVLRRFWDRKNFRYTRDSPFHVMITSYQMVVSDATYLQKMKWQYMILDEAQAIKSSQSSRWKNLLSFHCRNRLLLTGTPIQNSMQELWALLHFIMPSLFDSHDEFNEWFSRDIESHAEANTTLNQQQLRRLHMILKPFMLRRVKKNVQSELGDKIEVDVMCDLTRRQDKLYQVLKSQVSTANYDAIENAAGSDDASSDQNLINTVMQFRKVCNHPDLFERADVSSPFGFVDFGKLATMAREGDTADVMYSTKNPVTYQMPRLIYEDIVLPSYENDIAARSKLLNFTLNVFNPITNKELCQRLTFLTGLEPSEFLQIAGKDVVQRAIEPLDPGKKPLIEEIYNENRSSNKLRNGLYVSSKPSTLQELSHSTTGGVLNSLLDVQSSVYNDQYFNNLHPSYRPGASSTPISIDVPGCRHLYEQTQRELFDPTISQALSEIPPETQYNLHVEKGIPLERFPVTGKFPGPFNKYFSSHISMPSMDRFITESAKLRKLDELLVDLKKNGHRVLIYFQMTKMMDLMEEYLTYRQYKHIRLDGSSKLEDRRDLVHDWQTIPDLFVFLLSTRAGGLGINLTAADTVIFYDSDWNPTIDSQAMDRAHRLGQTRQVTVYRLLVRGTIEERMRDRAKQKEHVQQVVMEGKTEAKKVRQLDDQSPNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5H3</accession>
    <name>A0A8J2T5H3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_14180g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_14180g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5H3"/>
    <dbReference type="OrthoDB" id="434695at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034044">
        <property type="term" value="C:exomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015374">
        <property type="entry name" value="ChAPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975:SF1">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09295">
        <property type="entry name" value="ChAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="533"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="533"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF88606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="778" mass="86758" checksum="EF920B6BC7AF2501" modified="2022-05-25" version="1">MFSQGSIPEVKEEAVGYSLSERRLKIPQFQDLGPPDLITMLKYIPSSSSNAADKSHHHGAGAGSASTSATPQAAGDAADGAGSTAQVNGTAGSADQSALLTGLHTGERLKGEIGTFFYCLGADTSDPTSIAVFLKSIADTISDTPQVWFGKSKNFSVARISLSTWNTFRKCDVSIVVHIPGTVQTYILDSNSEQLQIGSSPADHDLVWTETFFSGIVRSVMLMKDNSEEGEVQNLVETYIVNPLTSGKLGNVVDIFIDLFPTVYERGHLLGGPCQVTNVTRTNNYLVETLVEIVRLTHSVEKCQAMLEKLIAKNPEAVVILVRVLLTNDREVDGMKLINETLASNDPEREEQPNSTTTPEPPAYLDYKSELLCVQTQFLIDIKKDYKLAEKTAQAAVNCSPSEFTPWSLLAKAYIRLGDVENALLTLNACPMTPLKEKYALKRVVPIPTEGSLHLPLPVDVILDEVSSLNPQDIQREHRSADPVLSNLAASNLKSVFQIAYRLLTEIVRLTGWETLLKYRSKIFVMEEEYQGSSDDINNSASAKASSVNVNETQDSQSVPHQEDGGEANTAESAVDTNNSTHALRSKRLCERWLDNLFMLLYEDLKTYTLWQTEQLYFEAQYSKYNKLTFEWELFGLCARRLGHYPEAARAFQKGLSQRFSSECARRLLEYCVKERQQIRDQSSSPNTEYTSSQIITRINELDHTIIDLCVKVCCWNHRWYIEFSIKLIDALSEVIQDMGLTKVSNEIAARYSEQVSQLMKDNMLDFFADYTNGYFDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5I6</accession>
    <name>A0A8J2T5I6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S04-06810g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_06810g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5I6"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005946">
        <property type="term" value="C:alpha,alpha-trehalose-phosphate synthase complex (UDP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000099">
        <property type="entry name" value="Alpha,alpha-trehalose phosphate synthase subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000036">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase subunit Tps2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF15">
        <property type="entry name" value="TREHALOSE SYNTHASE COMPLEX REGULATORY SUBUNIT TPS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="38"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="130"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="220"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="49"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="270"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF89542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1071" mass="119366" checksum="56A18643F1A68690" modified="2022-05-25" version="1">MKIIIASLFLPDQPQFELEGDQQDGVGWVDPNLVKVNNPSTEHLRRTRNGSTNSNSTPYSGGNASQEFLGSAESEQNPQSGPFNMEGPISSEKFMENLTANATAAHSPVTMGNHRAHSAEDFFTNPANGHKMTSGKPSMPETPESLQPNSSHDSTANLLKNVNKSLLQQSVLNNANNSSQMAMEGASKFPMGNSSSTVVTPKSRAMPSVNHAVVNFAKVKRQQQQATLPSVRRVPARASLGNNGSGSSNLKFSQLASEEAAEENAVSGSESEEGENEANSDSDLESDGEHEYFVPKFGGYSNNAKLRGSILQSSHELFSRLPWKIVPSDKGNGALKNAVFTAVRERTIKDPVRFVGTVGIPTDEIPKEVSAKIAKTLEDDYSSNAVIMDDVTFKGAYKNFCKQILWPTLHYQIPDNPNSKAFEDHSWGYYQKLNQLFADRIAEIYEEGDTVWIHDYHLMLVPNMVRKAIPNAKIGFFLHVSFPSSEVFRCLAQREKILKGLLGANFIGFQTKEYSRHFLQTSNRLLMTDNNENELKYKGKIVSIKNIPVGIDFFELNSQLRNERVCQWRELIRGRWKDKKLIVCRDQFDRVRGLEKKMLAYERFLRDNPEYVEQVVLIQITMGSEKDSIAERNIMLVVDRINSLSPDVSVSQPVVFLHQDLDFAQYLALSCEADAFIVDPLRGGMNLTCHEFVSCSEDKNSPLLLSEFTGSANVLKGGALLVNPWDIKKVSQAIKKALEMPHYEKRQRWKKMVKSIINNDSDNWITTSLQEINNAWEFNSERSNVLKLSYSQIKSDYESSKKRIFILKISEPPTPKVLEILNDLASKDCVYVLNSFSKTTLENLYSRVPSIGLIAENGAYVKLNNVWYTIVDQTDWKDDVVRILDDKVERLPGSYYKISDSMIRFHTENAEDQDRVVGVVGDAITHINTTFDGRGIHAYIHKNIMFVQQRGLSLTAAQFVLKYHNTKSSASTPVAPADVGVTSSPTKIGQGGSYFSGAGSSPFHVDFACVTGSSSPVLEPIFDLIRQEVKKGSLRFGYAIVYGHVSSTFATGHVDGLNELLSILESISKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5K0</accession>
    <name>A0A8J2T5K0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_09296g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_09296g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5K0"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF88398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="552" mass="61628" checksum="2DCC70698621BE6C" modified="2022-05-25" version="1">MVKQNTVLLSHSVCEKRNTPRAKVLTPSLKRDSVGEVWLCDYYPHGLRESTFTSKGQWFKLKLAKCMSHVNDPATTCSKGNPANSNENLQSAEGIENPKCESSFDEGSTRSAIAPADDFLIANETCKVPNMNIAVKPTQCVALDGLASVGKGTSKLKQIQDIKFMESKENSATEVSKEFLASNFEAIDAMIQATTAGNIGSRKGTPPFKNRTRRIRRRRKVKDKGRDCCKQVAYKVPIKEHYLKRWLDSKISPKHLHVNDSDQRSGSVRMFNNSSSYGTRITRRIDLKTEQSADIAHIKIHSDQHVGDIDKQQEGSFETESVYESTGAKTSCHNEIGVSMEIDDEEFNPDLALEKHSRIPILLSQLESLTPKSTLLEMLTLNNKFVGNSSKAENSQEKKLSSTSNAGLQSGNILISPFPVEMASRSINLDGEIDEKKEDFGLESFLLPQSLQLQGNDRYQDLDTDVSMRGFTGQFLTSACNTWELFDKTEKMDEPFEIADEQTPNCSLHNISESFYKQLKSDKGEVYRSIIPIPASQAIISPYSDDKTGYAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5Z8</accession>
    <name>A0A8J2T5Z8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">BN860_16160g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_16160g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000604">
        <property type="entry name" value="ABC transporter B family member 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001471">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF15">
        <property type="entry name" value="ALPHA-FACTOR-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="263"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="722"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="765"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="844"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="869"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="947"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="42"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="365"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="726"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="1052"/>
            <end position="1287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF88690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1290" mass="144625" checksum="B08A15656542EFEE" modified="2022-05-25" version="1">MYPIYGIRINLTTIIVRHCWMIAKGGSIYHYVACKRDKWLLLVIVSSTILSGIVPAVVSILTGRVFNLLNDLNRRSHPMQELTVASMSILVVGAGSLGIIWLCITSWMLLGETQHFEARNKLLSSYLIKPMEWYDKNDSIEGELTQLNRCVEELRSSSAEASALTWKNVVTIVALLATSFYYSWSLTLITMCSAPVIIICAALFSHMINIHAERENESTSKSSQLLTWSLEAAQLVKLNCTEKLEISSFQISVKKCSINFIKLSLYASINTAILKFLTLTMFIQAFWFGCTMIRKGELIIADVITCFQSCIMLGATINGTLQQIVVLQKGQVGVRQTRAFLALDEKFDELKNQSYSSPPHFTEKVEFCGVQFSYPSRPSMPALKNVSIEFPVGKTTFVIGKSGSGKSSLANLLLKFYQNYKGDIFIDGTNIKVINQSTIIENIMVVEQRCHLFNDSLRNNILFGCRDIDEVAMSEKLKAACRIALLENFIHDLPYGLDTAIGSEGIMPSGGQQQRIAIARAFMRDSSILILDEAVSALDVVHRGLLMSAIRTWRADKTTIILTHDLSEIQPNDMVYLVEDGEVLEKGLQKELLSNHEGRFFNLANIGLSDDFDDVYSQITTVETQKAKIPLPSYEDSLIEYEVETPRMDEECGSLFEVSSIPLEDLSFRQKKRCKTLRVTVQEEFTEQDSVEHIKVESANNEELVSMLEIMKHMYTNGGRKYILYVGIICSLYAGVANPIFSYTFSYLLNGIVPDGETHGGSAGYLLKWSMIVMGIAAADAVLNFVKDFVLGYYSETYIRMLRNSAMRKITFNRLAWSSKIKPSELSALIMNDLRDLRTLTSKFVNAVSSFLIVSSVGLIWALITGWKLSLVCVSMFPLMIIFSVIYGNLLKKYETEYKNTIAKLENHQYEIVKGIKTIRNLQIESHFMGKFKELERQAKGIGRRRAIVIGFGVAGSNTLTMVVQGILYYYAIKLVITKEYTSKRMFETFTLLLFTIMTCNNLVNQIPDIRKGQRAAHKIYRILAQDESCEHAGSYPCRKTPILSNHHGPLISFKDLSFEYPSTPTVKILKHLNLDIYGGDRIAIVGESGCGKSTLINLITGLYESPKNSLFIDHTDLHDWALFDLRQQIAVVEQKPVIFSGTIRENLIYGVSSNIQDIDLFDMLKYVGIYDFISTLPDGLETKIDSALLSGGQNQRLCIARGLLKKPKILILDECTSALDAVSSNIINEIVRVGPPAIVTISITHDEQMVKACNKVAVLKNGCVVEIGDPEELLNTRSNLSKVLTRIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T621</accession>
    <name>A0A8J2T621_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase PEP5</fullName>
            <ecNumber evidence="9">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_00408g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex. Component of the class C core vacuole/endosome tethering (CORVET) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Vacuole membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the VPS11 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="9"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T621"/>
    <dbReference type="OrthoDB" id="26184at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033263">
        <property type="term" value="C:CORVET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048284">
        <property type="term" value="P:organelle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16688">
        <property type="entry name" value="RING-H2_Vps11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057308">
        <property type="entry name" value="CHCR_PEP5_VPS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057307">
        <property type="entry name" value="PEP5_VPS11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016528">
        <property type="entry name" value="VPS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024763">
        <property type="entry name" value="VPS11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323:SF24">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23341">
        <property type="entry name" value="PEP5_VPS11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23356">
        <property type="entry name" value="TPR_PEP5_VPS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12451">
        <property type="entry name" value="VPS11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007860">
        <property type="entry name" value="VPS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0926">Vacuole</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="CHCR" evidence="11">
        <location>
            <begin position="406"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="931"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="884"/>
            <end position="918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR007860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF88721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1032" mass="117361" checksum="BB4D1EECFC01BB04" modified="2022-05-25" version="1">MAFASWRQFQFFESTPIRDPLQGSDTPLYSDPTLSAATPINKNQFALAVKSNYIRVVSLSESRIDHEFQAYEDNYQITYLEVVDQGFLLAVGEKLGKPSLIKIYKLNNLPSKPSSYHSIVEVKKGNYTYPISVVATSRDLDCIGVGFVSGKILLIRGDILRDRGSRQRIIYEDAGKEPITSLQFNLESTVCFAATTTRVMMFSTTGRNRGQPDITLDAQHGISLNCSCFSSYTDEYICYVEGSIDIYTEGGEKRSVVTGLSGVKRISSIDEDHLLLVVEAEYARSTVLEVEEFSQSNSNRVIILDLKNQVVTLNNFISSAIIDIFIVSDGNYKLVYLLTSEGTIIKICEKSLEDQLDIAIQKEFFDFALELAKQHSLDPLKIQEIHRKYGDWLYKKGSRSEAVEQYIQCLDVVETSEIISKFGINESPDPKGLQNLAQYLWSLIKENRSNSDHITLLLIALVKLKAKNDIQYFIDHYSRSGNFSEDVITSDMDDESFFYSDKNLFDLELVLDLLQESKFEELAYRMACKFAKDPVVIVDLILNTLDDPHAAIRYIRSLSIDETLRVLVTFSKQLLDKCPNDTNILLIAVFTGKFKRTEFANNLKASEPTHELSDDLMTVFYSYRTFLKYINNVTGGEAEVEARNSTAPTYHPPKASLIFNSFISNPFQFVVFLEACLESYQRYEGSKEDKQVILTTLYDLYLTLANDDIPERQNDWRSRATKVLKESNRLFMASENATAKGSSSGKSVDNSLMMLISHMNQIDIHPIQEGDGEKKNISELDKGSRASLLNTFRFMILTDDPQKCLAFMEAHGVQEPYLYCVALKYFISTKQVLEQIGGEKVLKDKILDKILEASLMSVVEIISVLGSTNVATYGLIQEVLISHVRQEQDQISRNKKLINSYESELKSKKKELKDLLDLEKPLQVTIKNKTCYMCHTALELPLVFFKCQHIYHQRCLNEEQKTKEGKKYFKCPKCIVELETSEKLLEAQREISKKTDMLKIALDNGDEIDDRFKIVTDFVGRGGLEYSHVTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T630</accession>
    <name>A0A8J2T630_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ZYBA0S03-09032g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_09032g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="360653at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052575">
        <property type="entry name" value="SSU_processome_comp_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057525">
        <property type="entry name" value="UTP20_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046523">
        <property type="entry name" value="UTP20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011430">
        <property type="entry name" value="UTP20_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17695">
        <property type="entry name" value="SMALL SUBUNIT PROCESSOME COMPONENT 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17695:SF11">
        <property type="entry name" value="SMALL SUBUNIT PROCESSOME COMPONENT 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20416">
        <property type="entry name" value="UTP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23099">
        <property type="entry name" value="UTP20_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07539">
        <property type="entry name" value="UTP20_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20 N-terminal" evidence="3">
        <location>
            <begin position="825"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20" evidence="4">
        <location>
            <begin position="1603"/>
            <end position="1819"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="1">
        <location>
            <begin position="1636"/>
            <end position="1671"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20 C-terminal" evidence="5">
        <location>
            <begin position="2244"/>
            <end position="2474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2457"/>
            <end position="2489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="2457"/>
            <end position="2472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF89095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2489" mass="285154" checksum="BDFD0CD548FF3B3C" modified="2022-05-25" version="1">MAKQRGTPKATNRFRYASFKDKIDDLKIEPARNLGIRAHDYVDTSHFLASFEHWKEINLSASFTGFAEEIEGIVQTLPQILFHQDKILDLLLHYIDKHEPLSVQPLLDLLAQFCHDLGPDFLKFYDKAIRCMVRLLEIAVTFESSDVFEWVFTSLAYIFKYLSRYLTESLVPTFELLFPLLSHSKPYLSRFSAEAMSFLIRKSSSKKLVGFVSYAFNKLEQSPESNLYDGLLTLFTESLITTQDSLHSKSKAILEILIEQVLGGNSEIAANLYSDLWIDIANHASSDNLAPIYENTWSMLQQRITSNNVEELARITSAMVFAESGKKISDWFQVIKVTKTIISLEDRSRLSAQTVAFLFMVLFRNCDIKNLTHFHKAMFEYYLHEYPGSFIQFFKSSINFCPERIFSFNGNKYLQQYINKHWKSEGERTALLFLQSDEDSEVGKLLHVNTPKEFSKSVLGSFSRYHQNISEDTLFDIGWGSIVLSKGTERDAALLISLLEDILGMPSSTPDFQSDIFGYLLDVLDSENLEGSFDLLKKILQQLKRYQTSLIFVKSLDKFLNQLEQTKELESYLTAHQEYILTLSDNLVLPDKELRYASLKLIGTLFQAQGKLVPQLINECEIIEEVPLTLQCARDINVRIRNMGATFMRSEPNKILNNTFLKFMFGLLTLRFSPVWEGVYEVLQNVYGKDQELVWNLIMKFLNGPDAHYNLAYRTEEVVLEHEKLCSWDVKAIRLHEALISFFHVWTNYSSPDTTILQTLKEKRANLDYPGQIRNQTLKFILMAPQLAERHSRDIVPYLFNQDESSEVFGMADLVEKDPFSAATWTEADRNMLLKVIGKFRNMKSIYKSQAVHERLMVLLGSRSTEVQRLAFEALLGYNNSVLTKYKDNLRNLLDDALFKDELIRLVSKGESRIIEDNDEQELMPYIIRIFFGRAQTPGTSGIKKGRKHAVISMLPNLGDPYIVEFLKLGTGKFNYDHFFENGYRIQESEADIATLRRMLGFVNIINSSLQVLGSAFSTAMATTIRPLLYTISMSYFVTSKEIKEPHVAKIGANLRQQSLKCMNNIFQNMSDSPTLLALLPDICEIVILPRLEKFEDENLQQPSSILKIMECWSTKEDLYPFLYYHGCVSPFAMMRVLSNENAKESVVSVILETANNIVSKPVEVEEYADLVTLVASNCLHVLPTLYKRLTDPAIVSISVDLLLNLVQAGYLQDSETKKCLVQSLTLIIREKFKGINKKDIGKILHVIASLMKEYECPWEDVKYLYEAICSLYAIYAERELREALNDVINSFGVNFPQLQKVAKFVCDLNSYSPKRMQEYDFPRILSSWKQFHEHQCKEFSDAQWLPVLFTCLFYINDEDELAIRTNASHTLCKFVDCMNEKPSFDDATTLIKTLKVIVLPNLRMGLRRAKESIQAEYLSVLSYIVTTGKYFTDLNDMRVLLYDGDEEANFFTNVVHIQLHRRLRAVKRVREHAPALSESSISLYLIPIIERYVFSQEEKFRNIGNEALVTIGSLANFVSWSQYKALLRRYIFVLKSKPELLKESVSLITKVSVALKNSLLHSRGKFNTSLSIRKFPDDLEGPEKFVVDEIYPKLSVVLRIRDNETAVARIPISEALVNFVFGLSEERTVSLLPGILTSLCQILRSRSEELRDAVRKTLAEISKVLGAEYLPFILKELISALRRGSQVHVLSFTVHHVLKTLATELKSSDLDNSSEMITNVIMEDIFGFAGEEKESDNYHTTMKEVKVNRSYDTAEMLTSNISLNFFSTVLGPVKALLLERINLKSQNKLNELLRRYALGLNHNAHASSVEVLALCHELFEQSENAVNVRAAKPSVTENEEFFLINLNAKKTKVRSENSLFNATLQKFSLDLLRTVLSRHRQLLGVKYLQGFMPFLKLSVASDNEDLLISGLRVLLMMVKLEFPGESEGMFLNCARKVLNIIKDSPSTSTEVCQVGLKFLSATIRQKDIKLKDSALSYVLGRILPDLNEPNKQGLAFSFLKALVYRHVQLPELYDVIDTVRQIMVTNHSKEIRDVSRSVYYQFLMEYDQSKGRLEKQFKFMIDNLQYPSQEGRQSVMELINLIVNKANSMLLSKISSSFFVALANVFFNDDAPRCREMASLLLTNLLQKLDSESLKPIEKYIIAWLRQGQEPTFLNLGLRVYKVYMLTLGLGKNLTLDDLAMSRMRSIVFDTDVGSEQPWDLIYSSLSVFLAYADMTEHVFASSMKIIWDKVIGCLLYPHPWLRQSAAKIVDILVTNLDRFETPFTELELQNIVSRIMRQLGAPSLPEDLAAVSVKTLMKIAIRWKQGNVAYIPKNSDQGREAKYSTPLNYMIVRAGAIIRSEDNPADSVMSKKSCIRLLGLLIQVLDENQLVNESENIILPLFKYLEQGTGERHNEEQEMLSMAAQECLQILESKISVSDFTKAYANVKQVVLSRRQERKAKRAVLAVTAPDIAAHKKLRKHTRSRQKRKHEKDENGFYQRKNKKKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6C2</accession>
    <name>A0A8J2T6C2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BN860_19284g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_19284g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6C2"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015254">
        <property type="term" value="F:glycerol channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000022">
        <property type="entry name" value="MIP aquaporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050363">
        <property type="entry name" value="MIP/Aquaporin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829">
        <property type="entry name" value="AQUAPORIN OR AQUAGLYCEROPORIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829:SF9">
        <property type="entry name" value="AQUAPORIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="356"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="496"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="527"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="578"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="164"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="217"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="642"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF87997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="663" mass="70959" checksum="0E3839C6920E8FE4" modified="2022-05-25" version="1">MEDRNNKGGKKPDIRLTTPKDKSAGKVTWEGKTKQSEGGTLPTNRRNRIAIAPLTPLTPLTKAGPEGDILLAEDSRRPGVVYAYSSPNLTALNNPSNYEQARLVTVQYENTGGSVRPTANYVDPLYRQLNPSVRSEKAKKPVWSLNQPLPHVLDQNLAGKVQKNLDAKSRASSRSASRAASRDVSRDVSRSGSKTSMSDWKSLLRRGPSYKRLNDLEAQLPSTSAASTRAKSERAAKRRQSQATLAPSVGDQGHHNATFTLGDGSSAVSIANQDSSMTEKPQVEAIETVPSHSQTLRRKLEQLDRSSVGNVGGGGGAVVITDGAGGGGDALADSDSEPMVFPNPWAKFRYYMREPFAECFGTLILVIFGVGGNLQATVSKGSDGTFGTPGGSFESLSFAWGFGCMLGVYVAGGISGGHVNPAVTISMAIFRNFPWKKVPVYIVAQIFGAFFGGAMAYGYFWSSITALEGGKDIRTAALTGACLYTNPKPYVTWRNAFFDEFIGGSILVGCLMALLDDTNAPPTNGMTAFVVGLLVAAIGMALGYQTSFTINPARDLGPRIFAAMIGYGSHPFHLTHWWWIWGGWLGPIGGGIAGALVYDLFIFTGCESPVNYPDNGYIEHRVNKIFHTNLLLSTAGSDHDDASGHSELADHNSAATADTLVKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6G9</accession>
    <name>A0A8J2T6G9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="19">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">BN860_01486g</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 19">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="19"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6G9"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000085">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000009">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF5">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 18 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 19" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="955"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1010"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1039"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1070"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1100"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="22">
        <location>
            <begin position="150"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="21">
        <location>
            <begin position="256"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="23">
        <location>
            <begin position="898"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="16">
        <location>
            <position position="498"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="499"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="500"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="500"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="586"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="629"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="653"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="682"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="763"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="764"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="765"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="845"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="851"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="871"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="874"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="875"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="875"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="CDF88065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1140" mass="128627" checksum="78D3109DEF05DEF5" modified="2022-05-25" version="1">MPLSPSRGLGADLNSKFGTSHNKSIDSFDLQFEDNLDRALENLQVRTSTHLERNDRSGQEEGESTESFELRTLNENDRLRDNMGRPSIGGSRPLMSDPWLPESGEVHDSTAASPIMVRHGILSKIISFFNLSPRYRSLSAIELNDSHTEREIHPETTPVYDRNKYPSNEISNAKYNAFTFLPTLLYEQFRFFFNLYFLIVALSQAIPALRIGYLSSYVVPLGFVLTVTMTKEAMDDIQRRRRDNEMNSEPYQVLNKQKLVKSKDLKVGDLIKMSKGDKVPADVVLLQSSEPSGEAFIKTDQLDGETDWKLRVACPLTQNLSQEDLLTKIRITASAPEKSIHKFTGKVNYKENTSNPLSIDNTLWANTVLASSGTCIGCVVYTGTDTRQTMNTTTATVKTGLLELEINSISKILCLCVFALSSTLVLLKGLHNRTWYIDIMRYLILFSSIIPVSLRVNLDLAKSVYAYQIEHDQTIPETVVRTSTIPEDLGRIEYLLSDKTGTLTQNDMQLKKIHLGTVSYTPDTLDIVADCVQSLGSPKNSSGPNTGRRDISTRVRDMVVALALCHNVTPSFEDDELTYQAASPDEIAIVKFTESVGLSLFKRDRHSITLLHEHSGKTFEYKILQIFPFNSDRKRMGIILHDQQKDEYWFFEKGADTVMTKIVEKNDWLDEETGNMAREGLRTLVVGRKKLTQKSYEQFQKKYESASLSMLNRDEQMSNVVAEYLEENLELLGLTGVEDKLQKDVKASIELLRNAGIKIWMLTGDKVETARCVSISAKLISRSQYVHTITKLDKPEGAMNQLEYLRLNTNSCLIIDGESLSVFLTYYRKEFFEVVSHLPTTIACRCTPQQKADVALVIRELTGKRVCCIGDGGNDVSMIQCADVGVGIVGKEGKQASLAADFSITQFCHLTELLLWHGRNSYKRSAKLAQFVMHRGLLISVCQAVYSISSGFEPIALFTGWLMVGYATCYTMAPVFSLTLDHDIDESLTKIYPELYKDLTEGKSLSYKTFFVWVALSLYQGCVIQGFSQKFTSLNELDFFKMVTIGFTALILNELIMVALEIYTWNKMMFISEVATMLLYVVSVPFLGEYFDLKFMSKPLYFGETTFILALSIFPVWAAKAIHRKLNPPSYAKVQEFSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6I6</accession>
    <name>A0A8J2T6I6_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">U3 small nucleolar RNA-associated protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_11606g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the HEATR1/UTP10 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="31183at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000530">
        <property type="entry name" value="UTP10p Nucleolar protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012954">
        <property type="entry name" value="BP28_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056473">
        <property type="entry name" value="HEAT_Utp10/HEAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022125">
        <property type="entry name" value="U3snoRNP10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040191">
        <property type="entry name" value="UTP10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457">
        <property type="entry name" value="BAP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457:SF1">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08146">
        <property type="entry name" value="BP28CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23243">
        <property type="entry name" value="HEAT_HEATR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12397">
        <property type="entry name" value="U3snoRNP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01036">
        <property type="entry name" value="BP28CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 9" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 9" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="BP28 C-terminal" evidence="10">
        <location>
            <begin position="1492"/>
            <end position="1639"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="8">
        <location>
            <begin position="1731"/>
            <end position="1769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF89203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1771" mass="199822" checksum="9276E7B6B73BE5AC" modified="2022-05-25" version="1">MSSLRDQLSQVSLQNAPVALDRKKRQKLHSASLLYNPKTASTQDYDFIFENALSSLKELIEIEPKFEIFTRTLFSDSSMSIDRSLQSKEEVKDLDKAINGYLMLASSKWHLAPTLHATEWLVRRFQIHICNAEVFLLSTINYYQTSIFKRILDIVKLPPLFHPLSSFVRTDKNPTHVTIIKLFSDLDFLKLYADYLSTCVRQGTTYTGQLLFSSCCFVNLIAYTQNNEERLNTMVPIVLEISAKLLASSSVDCQISAHCMLVVLVTALPLKKAIILAATETILSNLQTGAKRSALITICKLFQTLKGQGNVEQLSDKLYKLFDAKYDVDYLNDHLGKPDSVPSDKFITTYLRALARYDYQNLSSITSLLQNIKLEKFELRLFITDLIHLSEVLNDKSQLISVFEYLVSLNETLVLNCLKSLNVSPEMFEIRLTTSLFSNKEEVSESDIMQALASEKIGNGKTTATLSFKEFTDKNSAFINTSNKSMLAESDDAFGKLLSLFLEAVRHRYSAGQFLSSFFTTLEGRITFLLRILVSPSAPNALRLISLNNISKCINSIDKESNVFALIPCMVCALNDISKNVRIGIKKLFSQISKRPFTKHYFMANRLYGEETEFPLLSPKDGELWLDHFLSEYSVEDHDISKLIIPKKNEKVYLLFWSNQALYIPLPLPKMVLIRNLINRFSTPASYSSIFENFINGYLANRDEWELCCRHNKTNFADFESVIVDLISPKEKNQFYVDFIINALKSDYDGFASIVASKLLKIFGTLKFEQQLHILQSILDTAAADEQSYDAIETLQSLPLTTELFVTILSQNRIHSDSEITELTKKRRRRSSTNKAALQNESVSQIAELHLKKLTIILETLNISKANGSDSLLSTLLSLLSDLETLEQDGGLPVLYTQEVLASCMLRTIHSIEQSSLPKPKHVQADVVVSAIRSSQSPQVQNKLLLVVGALASLDFETVLHSVMPIFTFMGAHSIRQDDEFTSNVVENTIKTIVPALLKGTENEAMDETGFLLVSFTTALQHVPKHRRVKLYTTLVESLDPNKAIAPFLVLIAQQYSSNITNFRLGEGRGLIEFTKSFMANFTVLQQLNGWGEYLSLIKVLIDAQKDDESKRELKLHALFTNNILNLSNNGLLALVQNGFNFMNKVLEESEVGYNDASSNFRVGLYSIILDPRSNTTLVNDVKSKFGMLLENVLNFIKNLNDFSAPMSSDTEDSSSENDAVDAKSDIKGALFTMLGQVLNLLPVDNFVDAVLPLLSSVNDKDIRYQLTLVINTKFEFTSAEVIPTAQRVMKALLQRIPLEQNYTDVLQVSFNTLASLIGQFGKSLDNSLLNECLSLSTRELNSSRTEVIISSMTVVTSCIESLGIKSIAFYSKIVPAALKAIDNNSQEVDHGNLRKQLQLSVLLLFASMVKHIPSIMMSNLQDVLQAIFLADEVETSIRLSIITLMVENVDLKEVLKVLQKLWSNKICQTEDSIAVSLFLSALESTIEAIDKRAATSQSPVFFKLLLQLLEYRSITNFDNNTISRIEASVFEIANSYVLKLNDKVFRPLFVLMVRWAFDGEGVTNTAILPTERLASFFKFFNKLQENLKGIVTSYFTYLLEPVDQLLKKFVNEDIVNVNLRRLTLNCLTSAFKYDKDEYWRSTSRFELICESLVAQLTNVESVIGKYLVKAIASLAANNNQADEHNKFMHKLLVEHMKSNCSSNEKLWTVRALKLIYSKVGESWLVLLPQLVPTIAELLEDDDEQVEREVRSGLVRVVENVLGEPFDRYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6Q0</accession>
    <name>A0A8J2T6Q0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S03-06854g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_06854g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRN3 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6Q0"/>
    <dbReference type="OrthoDB" id="26970at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001042">
        <property type="term" value="F:RNA polymerase I core binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001181">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007991">
        <property type="entry name" value="RNA_pol_I_trans_ini_fac_RRN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12790:SF0">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12790">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IA RRN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05327">
        <property type="entry name" value="RRN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="603" mass="69803" checksum="EF2AA3CD6E0B8F32" modified="2022-05-25" version="1">MMAFGEQTGKRTGQDPIGLEPKKRKVEFVVPEDGDDKEFTAEMYKRFLKTALSDLEKNETTQLDMVANQLAQPANSDQRISAENLSVLLDVLSKNINKIDSSKRAPLIQSIINFEKWWELPSYTLNKYMLFVTILCSSIPKWWQDVSMSLVSGFNLSPEKTVHHHSLLKYFLQMIPSSMGYIDMYLVRFFPNNNDSSKNLVNYISNLLTLTTYCKELQFQAWSLIVERTISLDVALQNELDELDDDIEDFEGDDDDDDNEEKEEDDTNGIPDEDIVAEDEDQDDVPESELEGEEEYNVKVSQSIKDLSTKLDAILTLVTGRLQEELTAENLQAGDGVTIFNTLTTLFKSHVLPTYYTKSIQYIVFLISQQQIELMDSFLVTLIDISFSPTETSEKKIKSLQYLGSYIARAKKLSRTQIIFVASYLTSWLNRYVLEREEEVDQPGGMERFKHFYAAFQALCYIFCFRYSLFRDADSSWECELDKFFPRMVLTKFNPLKYCNENVMLMFARIAQHEDVACCFSIIENNNNERLRGIIGRGDKDSKTSQSVATSTWSLATRQQFIDLQSYFPYDPLFLKHYKQMMKDYYIEWSEAGGEYESDELED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6W6</accession>
    <name>A0A8J2T6W6_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Elongator complex protein 3</fullName>
            <ecNumber evidence="15">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">BN860_06370g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalytic tRNA acetyltransferase subunit of the elongator complex, which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>uridine(34) in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine(34) in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.3.1.311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="15 16">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ELP3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="15"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10265243at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000003">
        <property type="entry name" value="Elongator complex protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056591">
        <property type="entry name" value="ELP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23613">
        <property type="entry name" value="ELP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01086">
        <property type="entry name" value="elongater_protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13 15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11 16" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15 16" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Radical SAM core" evidence="18">
        <location>
            <begin position="91"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="17">
        <location>
            <begin position="405"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR005669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005669-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CDF88279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="583" mass="66575" checksum="6C39E1328623661F" modified="2022-05-25" version="1">MAPGRKGPKTNKPKLAPEKERFVQCCSDITLELTNSLTSGTTKETNLNGLITKYSKKYRLKQQPRLTDVINAIPDQYKKYLLPKLKAKPVRTASGIAVIAVMCKPHRCPHIAYTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPYEQARGRIEQLKQLGHSIDKVEYIIMGGTFMSLPKEYREDFVTKLHNALSGYNGNEIDEAIQYSQESVTKCVGITIETRPDYCTPTHLDDLLKYGCTRLEIGVQSIYEDVARDTNRGHTVKAVCETFSIAKDCGYKVVSHMMPDLPNVGMERDIEQFKEYFENPAFRTDGLKIYPTLVIRGTGLYELWKTGRYKSYNANALVDLVARILALVPPWTRIYRVQRDIPMPLVTSGVDNGNLRELALARMKDFGTKCRDVRTREVGIQEVHHKVHPDQVELIRRDYFANGGWETFLSYEDPKRDILIGMLRLRKASKRYTYRKEFTGQRTSIVRELHVYGSVVPLHSRDPRKFQHQGFGTLLMEEAERIAREEHGSEKISVISGVGVRNYYTKLGYELDGPYMSKQXXXXFFFWPSVNSNSAPTAILPWAPEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6Z5</accession>
    <name>A0A8J2T6Z5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="16">ZYBA0S03-10506g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_10506g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S9B family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6Z5"/>
    <dbReference type="OrthoDB" id="16520at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002471">
        <property type="entry name" value="Pept_S9_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF200">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 10, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00708">
        <property type="entry name" value="PRO_ENDOPEP_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="15">
        <location>
            <begin position="147"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="14">
        <location>
            <begin position="605"/>
            <end position="800"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF89158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="803" mass="90566" checksum="5C19399F2A7BD79D" modified="2022-05-25" version="1">MDAAEKYKLWDLEAAEEEANPKRTRLRICSLGLALIGFIFITVSIFREETLTFLKSQSSHGQLTKTGQVKVSFENVRNGTFAPVMKALQWINAPGSISDDKGLFLTNSDDGYEVRSVFDKDFSRTLWKGKSFVYKNANYSVDSLVASPNLEKLLIRTKTVSNWRHSSFGSYFVYSNSTGFQCIGDNLSLAEWSPNSLDIAYVHDNDLYLYSSEESKTVDRITQDGSSQVFNGKPDWVYEEEVMETDRTLWWSPKGDYLAFYKINETQVGEFVIPYYVQEPEDVYSKLKSIKYPKSGTPNPAATVVLYDLKKGKASQVELAASPDLLIETKWVGDRMLLVKSTDRSSDVLTVHLVDAEAKESKVVRKESSNGGWWEITHNTLHVPQDKSKGRASDGYLDVLPVNGFNHLVYFASADDAEPVILTRGAWEIEDGPFSFDQETNNVYFIAAKKASTQRHLYSVSLQNPEKIVDITDTSTSAVYSATFSSGGRFVLISYKGPDVPFQKIVDLKSDEQDVEKEGNIVGKTLYYLTKNEELRRNLASFAIPSKRVQELTLDDGVIANSFEILPNDFDPQKRDHYPVFFYAYGGPNSQQVLETFSVGFLEVVASQLNALVVVVDGRGTGFKGKEFRSLVRDQLGDYEARDQISAAALYASKPFVDADKISLFGWSYGGYLTLKTLERDAGAHFKYGVAVAPVTDWRFYDSVYTERYMHTPQQNIQGYVNSSVHNVTAIAQAKRFLLMHGTGDDNVHFQNSLKVLDMLDLNGIENYDVHVFPDSDHSIRYHNANTIVWDKILNWTKKAYLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T776</accession>
    <name>A0A8J2T776_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Peroxisomal hydratase-dehydrogenase-epimerase</fullName>
            <ecNumber evidence="5">1.1.1.n12</ecNumber>
            <ecNumber evidence="6">4.2.1.119</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Multifunctional beta-oxidation protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">BN860_06084g</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Second trifunctional enzyme acting on the beta-oxidation pathway for fatty acids, possessing hydratase-dehydrogenase-epimerase activities. Converts trans-2-enoyl-CoA via D-3-hydroxyacyl-CoA to 3-ketoacyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a (3R)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32711"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57319"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="1.1.1.n12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a (3R)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:26526"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57319"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="EC" id="4.2.1.119"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.n12" evidence="5"/>
    <dbReference type="EC" id="4.2.1.119" evidence="6"/>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF89978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T776"/>
    <dbReference type="OrthoDB" id="3592703at2759"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018812">
        <property type="term" value="F:3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03448">
        <property type="entry name" value="HDE_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05353">
        <property type="entry name" value="hydroxyacyl-CoA-like_DH_SDR_c-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000410">
        <property type="entry name" value="Peroxisomal multifunctional beta-oxidation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000185">
        <property type="entry name" value="peroxisomal multifunctional enzyme type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054357">
        <property type="entry name" value="MFE-2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051687">
        <property type="entry name" value="Peroxisomal_Beta-Oxidation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024">
        <property type="entry name" value="DEHYDROGENASES, SHORT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024:SF2">
        <property type="entry name" value="SCP2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22622">
        <property type="entry name" value="MFE-2_hydrat-2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0456">Lyase</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ketoreductase" evidence="21">
        <location>
            <begin position="10"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00055743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00073871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00081853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CDF89978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="921" mass="99963" checksum="D4F3CDC28BB16FF7" modified="2022-05-25" version="1">MSSKLSFEGRVVVVTGAGGGLGKVYALEYAKRGAKVVVNDLGGSLGGSGHDKRAADLVVGEIRKSGGTAVANYDSVSGGGSNIVKTAIDHFGRVDVVINNAGILRDVSFAKMSAEQFAQVVDVHLNGAYELSHAAWPYMLKQKFGRIINTASPAGLYGNFGQANYAAAKLGLVGLAETLSKEGLKYDIRANVIAPLASSRMTEDILPAHILKQLGPEKIAPLVLYLTHESCKVTNSIFELAGGWYGQIRWERSSGQIFNPNEETLTPEALLHKWGSINDFTDKPFKPTEHPIQLADYNDLITKARNLPKNNDQGTIKIESLQNKVVIITGAGGGLGRSHANWFARYGAKVVVNDIKSPKDVVQELNNKYGEGTAVSDTHNIITEAPLIVETALRSFGHVDVLVNNAGILRDRSFKKMVDSEWNAVLQVHLFATFAMCKAVWPVFMKQKSGYILNVTSTTGIYGNFGQANYSAAKSAILGFSKTIAIEGASRGIIVNVIAPHAETAMTRTIFRDTELKNHFDPSQVSPLFVLLASEELQRSKGKIGVRGELFEVGGGWVGRTRFQRSAGFAAVASTKDNIITPELIRENWSKVVNFSNPQYPSSFAESSALILRALQAASSPHTGNSPTDSKNTAKSTSPDIFVYTDRDSILYNLGLGAKSNETQYVYENDPHFQVLPSFAVIPFMHNVMTGLNMGELVDNFDFSKLLHGEQYFKLFPPTVLATHRSVRTIIKPLQVLDKGSKGAVVVGGSETFCTKTNKLLATNEGTFFIRGATARGSKARSLGARAKFATMNFKPPQGQSPDAELDCFTSRDQAALYRLSGDYNPLHIDPKAAKAVGFPRPILHGLCTLGITVKALYEHFGAFSELKLRFTSPVFPGDHLKVRVWKQANGTVIFQTVDVTRNVVVLDNAAIHLVGSDSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7J5</accession>
    <name>A0A8J2T7J5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ZYBA0S06-01838g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_01838g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7J5"/>
    <dbReference type="OrthoDB" id="1914839at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032936">
        <property type="term" value="C:SREBP-SCAP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045540">
        <property type="term" value="P:regulation of cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030225">
        <property type="entry name" value="SCAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46378">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN CLEAVAGE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46378:SF1">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN CLEAVAGE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="400"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="469"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="585"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="12">
        <location>
            <begin position="247"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF90151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1241" mass="140355" checksum="430F4EEAF7225E4E" modified="2022-05-25" version="1">MRYIQSAGHSGCYGESSHLCWQRHFFKFCARVVRHPRIHVVLPLLFAVILSCKLLTYVVHPSQAMAKLSESQFVCKVELLKDTASNPVESQLSLVQIWMRSTEDAANVLKKRILLESLALQNKLLEGVKVEKLISSPFQLWSNSLRRLQSDPAPLRTINSRLMALPRYSLFGTWKINGLISSAAGITISLLVNNTEYPQLQATLNQNAAKLNDMRNVTNFHIFAQPHMDSIKTESLQLFLVKLGSKYKVIIFILHFALLLHLALSMKRLSKTVKSVLGISLAMVSQIIFTLASATTVTKFCFKSSSDYIPIRALSCAVIFFSLNEQIRLLEDTRGCSFEPKPAEDPERISSEQFYDNCDSQVDRSFVSHAASCHLSSTKSLVVLSLIVTVLIPFSRRTTLFLLLSFWFGHFLQCTFFTAVLSLDRRRWEDSDLLTLDNSLLLDDNISGRSKFDFENHLHSLRFICRNRYSVLLTAIYLLAFNIKFSVARSSSSLISKLFHKGFRHVLMFGKPVPHVIFDQRFIAEGTSKLINEANKKGCYLANLIVRDPFLVLKYQDGQNLSQLEKQLPTIFNTSSFTSSYKLDFYYVFEFLLFVILLLSCTMLILQKLTEKLDHINSFNLLGNSGLEDGIDIEDAKRSKDGKKYLQNLEDAQNVNGVDDTNIEITDHFHAKELYKGGHSLDIVGIATSKCPFVVSVGLDYKVYVWSPLLKPIPSPTCIPLPRKFWPLSKVVLSNDGYYVGFFGKNGSITTWSRRQMNFVWELQLKKNCQSKDSSVTAPPLEAFFRKITVPSARQKKLPTTTMLRPELGSRRSSVNSIDYAARLTEGGIDAAYEKVVFTHSDAEEKDELVFVTPSGFIYSIQTSGELNVEKLTVSDHSLKSCKLLSSPRVNDRLVACDMVGDLYVSTVVNNKWRQRKLPINYNGMLAPSSNFCEIKENSPGSPTSQETRLMSKTDYTIELVSFVGLILRVIGREAELIDAMTGTVIRTFTIGLFKAGSLRVFHDNPTHCRFCGSASVASFSIAYNSFENERRVTLHTFKLESRTKNSICMRVERDPREIRCLGMESTFETIHYFYDAEDWCVTDNNMLIGIRRVPQDIPAKVGTTSISKIVTEDDESGVLMKRKPLKSAGEQKTVRNSEFCIHNIWEGWTMSAAGKISLHKIPMGVNGLIVNRLGPVARYGAKAMIVGFANIMNLFYIGHEELIFNPEKEDANQEELSLRFVNKRRDRLSSKKSPLNYSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7T4</accession>
    <name>A0A8J2T7T4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aminopeptidase</fullName>
            <ecNumber evidence="11">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_02212g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="11"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7T4"/>
    <dbReference type="OrthoDB" id="10031169at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000006">
        <property type="entry name" value="Puromycin-sensitive aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF171">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="14">
        <location>
            <begin position="16"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="12">
        <location>
            <begin position="255"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="13">
        <location>
            <begin position="545"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="331"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF88800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="880" mass="100587" checksum="1E7EC1C67B5EF4A9" modified="2022-05-25" version="1">MANSEKAFTLPDNFRPRSYEISLSNLDHKRRIFTGHVKIHFSCNKPSKKIHLNVRDLVIDSAEIRLVSDGRTLQLLSCETNSRLEAITLNVAEPISSNFSVHIDYRGVIQNNMSGFYRSKYKEHKSRKEKVMLSTQFEATDARGAFPCVDEPAQKATFKVSIIAEECYTVLSNMPEEKRSSRGSQVEHIFHTTPLMPTYLVAWAIGEFEFIEGKTEKEIYPTLDQYSIVDGTSKTTGVLPIRIYTSLGKRNQGLFALSVATKAVDLFSELFKIPYPLPKLDLLCVESYSHNAMENFSLITFRPSALLTNYKNGGDSNLAALEKVAYVVCHEIAHQWFGNLVTMQWWDELWLNEGFATWVGWYAVSLLFPHWDVRSIVMLRSHETALTLDALRESHPVKVTLRDPKDIDQVFDTISYLKGCSVLEMVSRYLGEKDFLAGVALYLERNRWGNATMEDLFDCIYQVSHVNMLENIRPWLLESGYPLLTVTESNDKLDIRQRQCGVCEEIKQTKYWWVPLLLTRDDEFLRKLDFTGKSIECGAGSKFNLLNTDGAAFYRVIYETPKLMEALCDNLINLSSRSKMSLIADVFALRPLANFLHLVSRIMMVHNSEDVYVWKMINTSLMKIRSVMFSPSDPKLLANFDLYILRLIDPWIEQALEYFRNCATISKNQEGASCPDSSIQFYDSILTTAGKLSHPGVVEAARALFLKGNINASNRAIVLTTVLSQPDTTRLIFDRVVEWLDKAVLEEVEIILRSLGGVTNPHLFESCFVLLFKIEQMNVQFLAEGWKDNPLIHSTILQSVRQYGRQLLNRLSVNQVIIARFVSFTFGYCRGKDSVQQIKLIFEGFDISAFDRVLNQTLELIEGNTGYYANNASLWKEFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7W7</accession>
    <name>A0A8J2T7W7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="15">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="15">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ALA1</name>
        <name evidence="17" type="ORF">BN860_04786g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 15">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Alax-L subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="15"/>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7W7"/>
    <dbReference type="OrthoDB" id="2423964at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070143">
        <property type="term" value="P:mitochondrial alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00673">
        <property type="entry name" value="AlaRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.130:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.310.40:FF:000003">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000011">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059090">
        <property type="entry name" value="ALA1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF9">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26023">
        <property type="entry name" value="ALA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 15" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 15" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="7 15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="16">
        <location>
            <begin position="9"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="600"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="604"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="719"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="723"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CDF90599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="956" mass="106718" checksum="8F23E82BDDDEB382" modified="2022-05-25" version="1">MTMGNTEKWTAKNVRDTYLNYFKSKGHTFVPSSPVVPYDDPTLLFANAGMNQYKPIFLGTVDPSSDFYNLKRAHNSQKCIRAGGKHNDLEDVGKDSYHHTFFEMLGNWSFGDYFKKDAIAYAWDLLTKVYGLPEDRLYVTYFEGDAEQGLEPDTEALELWKSVGVDESHILRGNAKDNFWEMGDQGPCGPCSEIHFDRIGGRNAASLVNMDDPDVLEVWNLVFMQFNREQGGILKPLPAKHIDTGMGFERLVSILQNCRSNYDTDVFTPLFARIQEITGARPYSGKFGAEDVDGVDTAYRVLADHVRTLTFALADGGVPNNEGRGYVLRRILRRGARYARKYMNYPIGDFFSKLVPTLISQVEDIFPEVAKDPSYLFEILDEEEVSFAKTLDRGERLFEKYADAASKSGSKTLDGLQVWRLYDTYGFPVDLTELMAEEKGLTIDGPGFEKAKKESYEASKRGGKKDAANVLKLDVHGISQLNNDKVPKTEDVYKYGMDNVDATILKLYDGSNFVDEISEPGQKYGVILDKTCFYAEQGGQEYDTGKLVIDDAAEFNVDNVQLYNGFVFHTGSLEEGKLSVGDKVIASYDELRRFPIKNNHTGTHILNFALREILGDDIDQKGSLVAPEKLRFDFSHKKAMTVEEIVKVENICNKQIKDNMAVYYKEVPLEKAKSIYGVRAVFGETYPDPVRVVSVGRSVDDLLSNPSNEEWHKYSVEFCGGTHVTKTGDIKVFVILEENGIAKGIRRIVAVTGSEAFEAQRLADEFSKELDAANKLPFSPLKEKKLKELGVRLGQLSIAVASKQELKTKFAKIEKGVKDEIKARVKQETKKTLDEVKEYFAQNETASFLVKFIDIPTNAKAITEAVNYMKSSAKDKSIYLFTGNDPEGKIAHGCYISDIAISKGIDGSSIAKQVSGIVGGKAGGKGNVFQGMGDKPTAAIDAVTELEKLFNEKLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T802</accession>
    <name>A0A8J2T802_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="18">ZYBA0S07-03466g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN860_03466g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Cooperates for the ATP-dependent vacuolar transport of bilirubin and glutathione conjugates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042592">
        <property type="term" value="P:homeostatic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18595">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000020">
        <property type="entry name" value="ABC metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000450">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000001">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056227">
        <property type="entry name" value="TMD0_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24357">
        <property type="entry name" value="TMD0_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="349"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="428"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="452"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="530"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="566"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="958"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="999"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1085"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1183"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="17">
        <location>
            <begin position="289"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="16">
        <location>
            <begin position="629"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="17">
        <location>
            <begin position="959"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="16">
        <location>
            <begin position="1279"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="858"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="858"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="870"/>
            <end position="885"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CDF90544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1524" mass="172816" checksum="2596A7A121F62215" modified="2022-05-25" version="1">MDQSNSSSWVCRLCRSKEGLGPISFYGDFTRCFIDGVLLNVAGLFMLIFGTWEIGKLWRKERPIFKYRKDWVIASRIFLVLLQIGFVSLSTLNLPKHKDEDITIASQYVITLLSLFDVAILHWIEYSRCNVSNAVVLFYWLLEALFNFAKALNFFIRHYYENKWPLGRTIFVITLFQGINASFILLLEALPEKKLMPYQQLQQKFSKRKSNPYDTANIFSRISFSWMTGLMRTGYEKYLDETDLYKLPENFGSGELSDRFDSHWQNQIRHKSHPSFAWALIATFGPQMLIAGVFKVIFDVLAFVQPQLLRILIKFVTEYGEQHKQKLYEFMGKVHLAKHANEVPLVRGFMISIGMFAVSFTQTSILHQYFLYSINTGMKIKSALTSIIYKKALVLSSEAADMSSTGDIVNLMSVDVQRLQDLAQWSHLIWSGPLQIILCLTSLYKLLGKSMWVGVVILLVMMPLNSSLMRVQKKLQKGQMKNKDQRTRLVNEILNNMKSLKLYAWEKPYKKKLEYVRNEKELKNLTKIGIYNALINFQFNIVPFFVSCSTFAAFVYTEDRPLTTDLVFPALTLFGLLSFPLAIVPLVLNSYIEASVSIGRLYSFLTNEELQRDSVQRLPRVSNEGEVVIKLGDNATFLWKRKPEYKVALKNVNYQVAKGELNCVVGRVGSGKSALIQSILGDLFRVKGFATVHGSIAYVSQVAWIMNGTVKDNILFGHKYDADFYDKTIKACALTIDLAVLPDGDKTIVGEKGISLSGGQKARLSLARAVYARADVYLLDDPLAAVDEHVSSHLIDHVLGPKGLLKTKTKVLATNKITALSIADSVTLLENGEIIQQGTFQEITEAVDSPLSKLIKEFGQKKHKSEDAEQSGSSSAESSSNHENSVPIKDELDQLQKLENLQLNEDGSQSLRRASDATLRSIDFDDEENAANKEHREVGKVKWEIYWEYARSCGVKNVLIFLVFAVASMFMSVMGSVWLKHWSEVNTRFGANPHAGRYLGVYLALGVLSAASTLVQTVVLWVFCTIRGSRYLHSSMANSIFRAPMRFFETTPIGRILNRFSNDIFKVDELLGRTFSQFFNNVIKVSFTIVVICFTTWQFTFFVIPLGMLYMYYQQYYLRTSRELRRLDSTTRSPIYAHFQESLGGLSTIRGYNQQKRFIHINQSRMDNNMSAYYPSVNANRWLAYRLEFIGTIIIFGAASLSIIRLKNGKLTPGMIGLSLSYALQITQSLNWIVRMTVEVETNIVSVERIKEYSEIPSEAPYIIEDHRPSPDWPTQGAIEFQHYSTRYRPGLELILKDINLKIKPKEKIGIVGRTGAGKSSLTLALFRIIEAAEGRIVIDGLPIDQIGLYDLRHKLSIIPQDSQVFEGTVRENIDPTEQYTDEEIWHVLELSHLKSHISSMGKDGLQTQLTEGGGNLSVGQRQLMCLARALLVPTKILVLDEATAAVDVETDKVLQETIRTAFKDRTILTIAHRLNTIMDSDRILVLDAGRVAEFDSPANLLKNSKSLFYGLCEDAGFVPSQRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T845</accession>
    <name>A0A8J2T845_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S03-06546g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_06546g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T845"/>
    <dbReference type="OrthoDB" id="10050400at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031942">
        <property type="term" value="C:i-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24145">
        <property type="entry name" value="Mgr3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040201">
        <property type="entry name" value="Mrg3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28142">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE I-AAA PROTEASE SUPERCOMPLEX SUBUNIT MGR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28142:SF1">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE I-AAA PROTEASE SUPERCOMPLEX SUBUNIT MGR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF88988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="481" mass="55893" checksum="470A95D23BF75677" modified="2022-05-25" version="1">MFKSGLRLTVRFANRLPKRYFHKQSSLANVSSSIGKQQRQQQEQQQEQQRKRLKNLKIERWAFSIAGLSALGYGIWYFYWPHHTFPVSVAKILRKGLWAESNKEGFDYQKAVGFYINALEECQKLHMDPISDEYTGIELKMAEMYEKLNMFEEANGIYLELLYRFYDALNSPGRVPEERRPDLIRRDLRILIKSLEISKDLNIGKRNLLAHLLLAQEEILSRSPELKEFFERRKEKVKSMFQGKVLNASDFKTFVNEDNIKLNDEGYMILDLQKNSSAWEPFKEEFFTARDLYTAYCLSAKDITAALSCKMTTVEWMVMADMPPGQILLSQANLGSLLYLQGEKFEAEIYQLEQRCEKDPAMKKEDIVIKTLRQLHKNRDTCLDMSKQCYDSVVQFAKRNNKLRFNVKDQLDPSVSQAIALSTYGMGVLNLHQGVLAKAEKLLKDSIALAKETDFSELLKEAENELQKTTTLKNKKQSEQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8E9</accession>
    <name>A0A8J2T8E9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="13">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">BN860_02982g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:21249"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="13"/>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T8E9"/>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000011">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.150:FF:000004">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1070.20:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000008">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000016">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1110.10:FF:000007">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1800.10:FF:000007">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009674">
        <property type="entry name" value="Rpa2_dom_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06883">
        <property type="entry name" value="RNA_pol_Rpa2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="17">
        <location>
            <begin position="40"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="16">
        <location>
            <begin position="202"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="18">
        <location>
            <begin position="478"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase I subunit RPA2" evidence="19">
        <location>
            <begin position="585"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="14">
        <location>
            <begin position="693"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="15">
        <location>
            <begin position="1059"/>
            <end position="1119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF06883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CDF90198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1195" mass="134664" checksum="7A843E25A421D8F9" modified="2022-05-25" version="1">MAKVITPPTTRTAHFRTVERENNFQNPPKDKSAYPLLYEAVQPHIGSFNALTEGPEGGLLNQGIKDIGEKVIFDGNRSEGTQLGNRLSISVEQASVARPMFNDGVASGVERRILPSEARQRLSSYKGKLVLKLKWEVNKGEETFGEVRDCGGVPIMLQSNRCHLSNMSPYDLVKSREESDEFGGYFIINGIEKLIRMLIVQRRNHPMAIIRPSFANRGAAYSQYGIQIRSVRPDQTSQTNVLHYLHDGQVTFRFSWRKNEYLVPVIMIIKALCDVSDREIFDGIIGPDLSNSFLTDRLELLLRGFKKRYPQLRNRTQILQYLGDKFRIVFQAPPDKSDLDVGKELLHRIVLVHLNGETNEDKCRMLLFMIRKLYSLVAGDCCPDNPDATQHQEVLLGGFLYGMILKEKIDEYLQAIIAQIRTDINRGIPANFKDRKYMTRVLMRVNDNIGSKMQYFLSTGNLVSQSGLDLQQVSGYTVVAEKINFYRFISHFRMVHRGSFFAQLKTTTVRKLLPESWGFLCPVHTPDGSPCGLLNHFAHKCRISTTQSDVSKIPSLLYSLGVTPASQTFAAGPSLCCVQIDGKIVGWTSHEQGRIIADTLRFWKVEGKISGLPLDLEIGYVPLSSRGQYPGLYIFGGHSRMMRPVRYLPLDKEDIVGPFEQVYMNIAVTPQELLSNVHTHVEFTPTNVLSILANLTPFSDFNQSPRNMYQCQMAKQTMGTPGVALCQRSDNKLYRLQSGQTPIVKANLYDEYGMDNFPNGTNAVVAVISYTGYDMDDAMIINKSADERGFAYGTMYKTEKIDLSLNRSRGDPISQHFGFGNDEWPKEWLEKLDEDGLPFIGTYVEEGDPIVAYFDDTLNRTKVKTYHSAEPGYIEEVNLIGDESNKLQELQTVSIKYRIRRTPQIGDKFSSRHGQKGVCSRKWPTIDMPFSETGIQPDVIINPHAFPSRMTIGMFVESLAGKAGAMHGIAQDATPWIFDENDTPADYFGEQLLKAGYNFHGNEPMYSGATGEELRADIYIGVVYYQRLRHMVNDKFQVRSTGPVNSLTMQPVKGRKRHGGIRVGEMERDALIGHGTSFLLQDRLLNCSDYTQTNVCRECGSILATQQSVPRIGSISSLRCRRCAIGFDEAKKLLAKYENSENAFIDDSHIWEDGQGNKFVGGGDTTTVAIPFVLKYLDSELAAMGIRLRYNVEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8Z7</accession>
    <name>A0A8J2T8Z7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_03576g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="HG316462">
        <property type="protein sequence ID" value="CDF91045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T8Z7"/>
    <dbReference type="OrthoDB" id="942095at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000650">
        <property type="entry name" value="Serine/threonine-protein kinase ppk16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="37"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="411"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="698"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="780"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="833"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="414"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="434"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="482"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="509"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="546"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="703"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="783"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="840"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF91045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="904" mass="101501" checksum="6E62D95577E2C34F" modified="2022-05-25" version="1">MAPTKVEQNQIKCIIGNSYNRLYSQFTSEELTEVGNYKIIKQIGEGSFGKVYLASHKPTHRKVVLKTSDKNDPNVVREVFYHRQFDFPYITKLYEVIVTETKVWMALEYCSGKELYEHLLVLHRIPVDECARLFSQIVSAVHYAHSLNCVHRDLKLENILLDKRGNAKLTDFGFTRECVSKSSLETICGTTVYMAPELTQRKSYDGFKIDIWSLGIILYTMINGTMPFDEDDEAKTKWKIVNEVPEFSDSYMNEDAKDLIKQLLAKSPGDRPTMDQILSHPFLQPYGQVMLARTEKIIKRQRNGMTQFHSRLERKLLKRLKQSGFDTQAIKASIQKRKCDSLSGLWLLLVEREKTCQRVDHSRRSRSVLSVKKVFDPTTSENNEPKSISLCQQSFPAPEVKKVSSLRRILSRNSENDPLKRPPLEPKKKYLEASQCQTRQTSIDPARNTSSSSVSSIKGSPGNKKNNILTKVTKFFKSRRQNNNNSTNVLNNNSNASNASNASVMNSSTGSQQYTDPPNSQGTSPSASTHAVESNECQDKKRTNRSESLNSATKSSNQSTAKEPDTAPSHANDASLGIPHQEQKPHNDHRLLKRFKSTTSSDISGQTSAGNYDVESVSNNNIQVPSHELPKSPSFLRPRPVSGISEFSNDTYNSEYSTDGNASSLRVADFPRPNFPITMSGEAAQDLQGRSIYKQTIERRDMSVMSSTSSASEKSSRTDSFYDITTASPPMLMDIRNSKGLSMADSVLPRFGAQHALFPKRNFSGSRRGNIGRKNHIRGFAKHNSSQPHSIIQEESSLNSQEEKHNSNGVTLPPDHNLLLEEENVVFRQNNVPASEKENPSSTPLAFQSEFDSPNFSSSNQVANNAEDWSPKYGTLHKGMSAPSLITEDDEALRIADQEDNFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T923</accession>
    <name>A0A8J2T923_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ZYBA0S04-08020g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_08020g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T923"/>
    <dbReference type="OrthoDB" id="6499973at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF315">
        <property type="entry name" value="TRANSPORTER MCH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="346"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="382"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="416"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF89594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="487" mass="53670" checksum="1738DEE5666BCD2F" modified="2022-05-25" version="1">MNASHKDEDLQSNYKAITVSSDVELTSKDRNFEPQSHSTNTRLEVPDGGYGWFVVLAFFIYNFCTWGANAGYDIYLQHYLEAHTFPGGGKLDYSAIGGFSFGVGVLFGPLIIWLQNVFPVQAVIGAGIVFQAAALILAAFSKKLWQIYLTQGVLLSFGLAFIFIPSLTLLPQWFRKKRSLATGIATSGSGVGGVIFNLGMYRMIEIRSVKWALIVQFIICLVLNNVALLLTRTRKEAVFQNSNIRTKFFDKKVCKNVGVWLVIGWVGITTFGYVVVLYSLSTFTTSIGYSAKQGSYVSCMVAVGWFFGRPAIGHISDRYGPISVGVLVHLILAILCWAMWIPCRNLATAIAFGILQGLLMGTIWTTIGAIVARVVGLRRMGLAFGLVWVSIGAFGIVSPIIGLVLRSGQGTKPTDFKRTCIFAGFGYFGASLFQWLLRGYIIARDRKIERNTGAINVNEDQDELDVSVKITDLIRSSFILRNVSKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T946</accession>
    <name>A0A8J2T946_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ZYBA0S07-03510g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_03510g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AVL9 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T946"/>
    <dbReference type="OrthoDB" id="26278at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018307">
        <property type="entry name" value="ABL9/DENND6_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051731">
        <property type="entry name" value="DENND11/AVL9_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31017:SF1">
        <property type="entry name" value="LATE SECRETORY PATHWAY PROTEIN AVL9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31017">
        <property type="entry name" value="LATE SECRETORY PATHWAY PROTEIN AVL9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09794">
        <property type="entry name" value="Avl9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="3">
        <location>
            <begin position="9"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="486"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="588"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="486"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="588"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="604"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="633"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="661"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF90545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="688" mass="78434" checksum="145A7B39CF2FA446" modified="2022-05-25" version="1">MKEELPVIMGLCLVDFHHTRGPEVEYWCGLPEGTDEDKIFPNLPFQALPDGSHSFKETFTYFTLLYNEKQRCSPPNGATDIPEDELKDYTTLFAISCSRQIRSDDLTYKEKDITRSTVQKAIVVISRQPIFGQIKDKVSLVTNAFFLQRDFTDKSIISSLYENLMSLFKTWDIQDGHIYLGLSLRKLIYDFKKDALFLLKSLLLEKQIIFYGNNVEALCNVQFGLISLIPNLLSNLQNSGSPLLHKNLDDLKVADSFRSSDRQSVLRFLGFPLSIFEKGGLFSPYTPLQQMQDIKSDNTQFFVIGSSNSLLAERKEELCDIFVNVDESSVEIIDKSLTAFLSLSNYDKKWIDSLTNTVISSWNENDLDTPRNSQFEGSEDFIRWQFEEYLTGLLSSIKLSDYIASPKENDTSLQSIPEDMQNSHPIQQFNSSWIQKWRETQNFKIFNKFTDDRIFDLFPPKHAYNATDPFTVFQQKLATTFQNLKKNNGSTDGTKVRDNTSISSKQQKENIITKTTSSVSSVGSNTADKEPNVWSFWKDYFKERRKNKKKEAAEANVKSTQHLEYSQSTNDAIDSALVGLGLYHEDVEASKDDGREEESKMEAAEEADEGGEVNEERARVVETEGEGSNRATDAQRENGNEVEHQGDREVGSDIEQAGRGGEQKDEIDGDKESYKSMRKSFTFEEVAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9D8</accession>
    <name>A0A8J2T9D8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G1/S-specific cyclin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_04544g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Essential for the control of the cell cycle at the G1/S (start) transition. Interacts with the CDC28 protein kinase to form MPF.</text>
    </comment>
    <comment type="function">
        <text evidence="6">G1/S-specific cyclin essential for the control of the cell cycle at the G1/S (start) transition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316461">
        <property type="protein sequence ID" value="CDF90847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T9D8"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007089">
        <property type="term" value="P:traversing start control point of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20559">
        <property type="entry name" value="CYCLIN_ScCLN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000080">
        <property type="entry name" value="G1/S-specific cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014399">
        <property type="entry name" value="Cyclin_CLN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21615">
        <property type="entry name" value="CYCLIN N-TERMINAL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21615:SF2">
        <property type="entry name" value="CYCLIN N-TERMINAL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001770">
        <property type="entry name" value="Cyclin_CLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 6" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 6 7" id="KW-0195">Cyclin</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="9">
        <location>
            <begin position="76"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="225"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="459"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="238"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00053308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF90847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="524" mass="59427" checksum="8307732829EE141F" modified="2022-05-25" version="1">MACDTRLGLNVTAKQTYYPIELSNAELLAHYETVQEYHQEISANVLTLSSKFKPDAKLIDQQPEMTPTHTRSAIVTFLFELSIMSRVTDGIFYHAVRLYDRYCSKRVVLQDQAKLVVATCLWLAAKTWGGCNHVINNISIPTGGRFYGPNPRARIPRLSELIHYCGGADVFDESMFMQMERHILDTLSWDVYEPMINDYVLNVDENCLIQYELYKRQLEHNKEWVKKRQSQQSHDSDATVDVDEDDEENVYEDEEDEDLEMKIQLINLKRFLIDLSTWQYDLLKYEMFEVSHGIFSMLSRFTDQDQGPFLMTPLPSTTNQAQVLNVFINAVAHTPASLLEVYKERAGVIDFVSTVKNYQADLHKKLQLASAMDLSRRSVSSARYFDQLSTAPSPTYSQHYTPMRNTSTQSDNSVFSTNMDQSSPITPQIYSFGQYANDGSTCGSACGSTLSVNSITNKNSYEQQQQQQQQEDKENQMATHQLPPRAKFLGNGMFASPSGTVNSANSSNRSSIISLAIGNVNTMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9J9</accession>
    <name>A0A8J2T9J9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1,3-beta-glucan synthase</fullName>
            <ecNumber evidence="3">2.4.1.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">1,3-beta-D-glucan-UDP glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_02718g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;3)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;3)-beta-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21476"/>
            <dbReference type="Rhea" id="RHEA-COMP:11146"/>
            <dbReference type="Rhea" id="RHEA-COMP:14303"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37671"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.34"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 48 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.34" evidence="3"/>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1880850at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000148">
        <property type="term" value="C:1,3-beta-D-glucan synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003843">
        <property type="term" value="F:1,3-beta-D-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006075">
        <property type="term" value="P:(1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051278">
        <property type="term" value="P:fungal-type cell wall polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026899">
        <property type="entry name" value="FKS1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056261">
        <property type="entry name" value="FKS1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003440">
        <property type="entry name" value="Glyco_trans_48_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741:SF15">
        <property type="entry name" value="1,3-BETA-GLUCAN SYNTHASE COMPONENT FKS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741">
        <property type="entry name" value="LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14288">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23605">
        <property type="entry name" value="FKS1_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02364">
        <property type="entry name" value="Glucan_synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01205">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="477"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="507"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="565"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1360"/>
            <end position="1380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1408"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1502"/>
            <end position="1522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1528"/>
            <end position="1546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1617"/>
            <end position="1639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1659"/>
            <end position="1683"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1695"/>
            <end position="1718"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1724"/>
            <end position="1750"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1857"/>
            <end position="1877"/>
        </location>
    </feature>
    <feature type="domain" description="1,3-beta-glucan synthase component FKS1-like" evidence="13">
        <location>
            <begin position="179"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="229"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="863"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1050"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1898"/>
            <end position="1929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="229"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="252"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1068"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1899"/>
            <end position="1911"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF90511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1929" mass="222693" checksum="CC1AC5C8B7D2027E" modified="2022-05-25" version="1">MDDCSPISLTDIDYPAWCQDEEVPVTKMELKSIFDDLARKFGFQQSSKENMYHHLLAQLDSRASRTTSQNALLSLHVSYIGGEQANFRKWFFAAQLDLDEEIGFQNMKLRGKSRQRNFKLAKKRGTSIKEQMEEWQEREQEFISNHPKVVLTPEQLEDKSNLRTADYKWRLKMKQLTPYEMARQLALYLLCWGEANQIRFAPECLCFIFKCALDYDVSTNLSVTETRRSAATAADAPRANAAMKSECDGDGDGNRNDEAEHDGNKESDSQRTKDKIKGEDGDGDARENESAPEYTFLQEVIDPLYHFLKTQLYRKNSKGKWVRRENDHKNIIGYDDINQLFWYPEGIERIVLQTGERLVDKRLEDRYLHLKDVEWSRVFYKTYREVRNWMHCLTNFNRFWIIHFAPFCFFTSFNSPTLYTKNYVQLLNNPPTLQARLSAVALGGTISCVIQILATFAEWKFVPREWPGSQHLTKRMCGLLLCLVINVSPSVYVFGFFELDVHSRSAYIISVIQLIVAVITTLFFAVRPLGGLFGSYLNRGRKKRRYVSSQTFTASFPKLSGRSKWFSYGLWTFVFLAKYIESYFFLTLSLRDPIRVLSALNMSRCRGDRLLQRYLCQWQPRVTLGLMILTDLGLFFLDTYLWYIICNCIFSIMLSFSLGTSILSPWKNIYSRLPKRIYSKILATSEMDIKFKPKILISQVWNAIIISMYREHLLSIEHVQRLLYQQVDSMMMDKRTLKSPTFFVAQDDSTFKTANFFPSNSEAKRRISFFAQSLSTPITEPVPVECMPTFTVLVPHYSEKILLSLKEIIKEESSKSKITVLEYLKQLHPLEWDCFVRDTKLLNIEKDTVNRLSKDLLHADSQNNDQCGDTDDNHSATGIHSTGNDERQQQQQQYGNRERENNAAPSLSFLTVDNKEENHEQDLVQKKINDLPYYFFGFSSSEFSYTLRTRIWASLRAQTLYRTISGFMNYSKAIKLLYRVENPSMVQLYGDNVEALENALSNMANRKFRMLVAMQRYTKFNKDEREATELLLKAYPTMCISYLWEEQQDQKQSLGKDPSQNEEEYQDTNKPVNSDASTTGADVSKSSNDKKDDDWNDDKDNEPVFYSCMTNGYADIDEKTGLRKPIFKVRLSGNPILGDGKSDNQNHSLIFYRGEYIQVIDANQDNYLEECLKIRSVLSEFEELDMESTIPYVPGIEYDEEPPPVAIVGAREYIFSENIGVLGDIAAGKEQTFGTLFARTLAEIGGKLHYGHPDFLNAIFMTTRGGLSKAQKGLHLNEDIYAGMTAVCRGGRIKHSDYYQCGKGRDLGFGSILNFTTKIGGGMGEQMLSREYYYLGTQLPIDRFLSFFYAHPGFHLNNMFISLSVQLFFLLLLNLGALNYETILCNYNKDMPITDLEKPIGCYNIQPALHWVSIFVLSIFIVFFIAFAPLVIQELLENGIWRATSRFLHHLLSLAPLFEVFVCQVYSNSLLSDITFGGAKYVSTGRGFAITRIDFAILYSRFANIAIYTGFQVFLMLVFSTVSMWQPALLWFWITVISMCFAPFIFNPHQFSFTDFFIDYRNFIHWLSSGNTQYERESWSSFVKSSRARYTGYKRKTINDVSEQDGKDINKATWDNLFVSELAMPFLVLLFDFSAYMFINAQTGVVDVAATNSVLRLLIVTFIPILFNMVVLLILFFTSLLAGPLTNLCCHNTGSVFAAIAHALSVIVYLVSFELMWFLESWNFARTLILLITCINLHIFIFKWFTVFCLTKEFKNNRSNLAWWTGKWYNTGMGWSVILQPIREFFVKIMESSYFAGDFFLGHFLLYMQTPLLFIPFIEYWHPMLLFWLKPSNIITSKVIYTRKQKRRRSVIVRKYFVLYFLVLGILLVLLVAPFTANRMIPDSSKLLASTPLIGVAQPNGQNNNDTGPNAPTTIMTTTPPMPQFRTVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9Q1</accession>
    <name>A0A8J2T9Q1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ZYBA0S08-05446g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_05446g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316461">
        <property type="protein sequence ID" value="CDF90883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T9Q1"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008053">
        <property type="term" value="P:mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051646">
        <property type="term" value="P:mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000638">
        <property type="entry name" value="Transmembrane GTPase Fzo1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027094">
        <property type="entry name" value="Mitofusin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465:SF0">
        <property type="entry name" value="SARCALUMENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465">
        <property type="entry name" value="TRANSMEMBRANE GTPASE FZO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="13">
        <location>
            <begin position="180"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="406"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="412"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF90883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="846" mass="96215" checksum="D3EB0A55A9F63CED" modified="2022-05-25" version="1">MSQKSLQPSHAGGVDDEDDDGSTVAPSQAPSHLSYSRRQGSFIVRDEDEEALDDSATVYSGNVHTLHPRKTSHVRSLSNERLLSAQLSQLNYNNNRVSLNRAILQVHDLLQQIEMENGARPIHMPLKDNGLRVLNLQLKIGGNYSERGEVTLDKKALAKLFETQLRDALNHLCSLQKRVDDVSSKVFITGDVNTGKSSFCNSLLKRRLLPEDQLPCTNVFCEILEARENDNVEEVHALSAERAQSVKGANAIYNIRDKSTYETFPLTDLDELVLQNDKYVLLKIYIKDDKRPAETSLLRNDTVDISLIDSPGLNMDSMQTAEVMTRQEEIDMVIFVVNAENQLTLSAKEFIALASREKKFMFFVVKKFDKIRDKQRCKDLILRQIKDLSPETYKSSSQFIHFLSHESGDPGGGDDGDDGDPYDNNDPDPDFDRLENSLRNFVLKRRSVSKLLPAKTYLCKLLCDIQLIAEFNIQAYQEEDEDLYRQMEDLKPKLARVKAGCEGLANSVDRLAEETVTDIYEFTRQRILSSLQIPLTDFPTYQGLSQIHDFIFQTEQFIKDRIKDCVLSSEVYAREQTQMSVERVSYMGKEELGDEFMSNRVFKSELMFTKKRHFFGKKLSVPLSMSQLFAPSWEGFFRYLSWGMGLPTAPVEADDEATASEQTKTDNGLTSALGLKNYSLVQYCRKPSLLFTSKIPTLAVYSFGGARVLRGVVIHGVQFFSWGAIKRIGGSVLFVGSLLSLAYLVHDLPRALPKNLSLRYKNALQDGDYVHANAERISREVREVLRVPTREIIRSCELVIDKKYGAQKELEKKRHDNALSMKYLQQIRERAVSQRQIVERINLEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TA07</accession>
    <name>A0A8J2TA07_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial Rho GTPase</fullName>
            <ecNumber evidence="15">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN860_05688g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 15">Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Mitochondrion outer membrane</location>
            <topology evidence="2 15">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the mitochondrial Rho GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="15"/>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2TA07"/>
    <dbReference type="OrthoDB" id="10020961at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01892">
        <property type="entry name" value="Miro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000553">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013566">
        <property type="entry name" value="EF_hand_assoc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013567">
        <property type="entry name" value="EF_hand_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021181">
        <property type="entry name" value="Miro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052266">
        <property type="entry name" value="Miro-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020860">
        <property type="entry name" value="MIRO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819:SF1">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08355">
        <property type="entry name" value="EF_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08356">
        <property type="entry name" value="EF_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037488">
        <property type="entry name" value="Mt_Rho_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51423">
        <property type="entry name" value="MIRO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="13 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 15" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="624"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="domain" description="Miro" evidence="17">
        <location>
            <begin position="3"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Miro" evidence="17">
        <location>
            <begin position="437"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CDF90638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="653" mass="74131" checksum="6AE756D22CB9BD56" modified="2022-05-25" version="1">MAKETIRVVLCGDDGVGKSTLIVSLVKGRFISNLQDVLPPVTIPRDFSSSPYSPKSTILVDTCNANQATLQRELKSADVIWLVYSDHDSYERVSLYWMMTFRSLGLNLPVILCKNKCDEYGENTAAVDSTDTKVEDEEFIPILMEYKEVDTCIKTSARTQFDVNQAFYLCQRAITNPIAPLFDARVGELKPLVVQALKRIFLLSDKDQDNYLNDDEITTLQRKCFGRSIDINELNFIKKALSDISSPTQEYDGYMLYVPDKGITKDGFLVLNKIYAEKGRHETIWGILRAFQYTDSLSIKDKALFPKLNVPDTSSIELSPKGYRFLVDLFLRFDKDNDGGLNDTELTFLFKCTPGQPKLWSETNFPYSTVVNSRGCITLQGWLAQWSMTTFLDYKITTAYLVYFGFQEDARLALQITKPRKMRRRAGRLYRSPLSDRKVFNCFIVGKAKSGKSSLLESFLGRPFTETYSPTLGPHIAVNSLELKGGKQYYLILQEFGGQENVILENRDKVKNCDVLCLVYDSSDPESFSYLVELVKRHEDLKDLPVVFVALKADLDKVQQRCYIQPDEFTEQLFVDHPLHISSTWPSSLNELFIKITEAALAPGKNTPGFPPESKINEAEYRQAVMIVASTVGFISLFTFTVMKLLKPFKDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAA1</accession>
    <name>A0A8J2TAA1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ZYBA0S07-00342g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_00342g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2TAA1"/>
    <dbReference type="OrthoDB" id="4151048at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052202">
        <property type="entry name" value="Yeast_MetPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782:SF10">
        <property type="entry name" value="PROTEIN SIP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="40"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="104"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="787"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="922"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1040"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="125"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="829"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="872"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1044"/>
            <end position="1065"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF90410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1065" mass="119765" checksum="43996FDC1FB9BFDB" modified="2022-05-25" version="1">MAVEKKGREGKRKRYGQPRSPRGEQALLMAKNKKMRQSQACDRCRLKKIKCDGMKPSCSQCAKVNFTCRTSDRLTRRGFPRGYTEMLETEVVRLQKLMESRGISIEPQDGDSDTKQIVTPSTNEEMSREPLGKEEEEKEFPGSDGTAVAGTHNPGSDGSAGTGAGASAGVGSVSVSNSRGGVGELEFPFSNDTFHYYDNYRRGENYMGNSTWDVLTGSLPSPSDEEPRNQTETDCFANYHLSAMKNILHLDSNVSFLPKFLLVKYDYNIPKLVKLLRLAIADFGKIENSLIPLLYPFDKWEKDLVRHLVQSNDFSNSAHSEGVDPITLLTLLYIIQLNWSCFNDFKLFQLTKTVCLSSKTRLETLQCILLASFYFMGGQTSAVIHQRSRDNSQVWATELLHIAYSIVLNLGLYVNSKRMIPVHANCDAAPQQHRVVTFWVFQFLDSWWSFLQGLPKSNFLVDEFHPQSIRSLNVAALTPFMLLLQFTVESLDGCNLLHILAGDHAKGRSKLVYVTESFRKMLVKWKLYHQMQDHEDDFDPDEAAHLSPTLTKPDMVEIQLTLTYLIVSFLSDEKFNDAGKQVTKNDVMTSTPTGFSSSAETTSLHSSNLEEIAYEILSLYSLVLMDHARLEQPQLLKILHLLSCDNRRLIHLCLSTLNNWALSSRGLGTSEGHTNWRYNRYRNMVNQWCKFYLADKLNDPLWQQLIVSFKLDLSAEQPQTFYNWNEVEYLENVEVFNGNPQVFSKSQLIRSNPHAVMDQFDIFSPTNVGGASGATNKPIFNNIQSTGFSRKMADEPQQVENPRSNISEHDSANIIDMSKYRSQVLTSNEETDDGYAEDDDDDDDDLLNKPLEIPLSRRTGSLFHRGSKNRNDNGSNYNTTNNNNGHRLSNMHPHNERLRRHTLDHIILDGPPSETMRFEEPVYSRQGEPASLDGADTTSDGKIPFQNYALDAKNNKRNRSQSISVPPHAEKAQGRPPPPQESQQQLPLGNTTIMPSAAEHGDVLFNGLVEDDSHDSHSSHSHFVETPRALVDMLMLPAEGQQGGSVVNGPSSCNSLAAGSSDTNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAD2</accession>
    <name>A0A8J2TAD2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ZYBA0S11-03004g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_03004g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316464">
        <property type="protein sequence ID" value="CDF91445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001147">
        <property type="term" value="F:transcription termination site sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21408">
        <property type="entry name" value="1B_Sen1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18042">
        <property type="entry name" value="DEXXQc_SETX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000326">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001152">
        <property type="entry name" value="tRNA-splicing endonuclease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044340">
        <property type="entry name" value="Helicase_Sen1_1B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024481">
        <property type="entry name" value="Helicase_Sen1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056474">
        <property type="entry name" value="SEN1_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF495">
        <property type="entry name" value="HELICASE SENATAXIN ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23576">
        <property type="entry name" value="SEN1_barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12726">
        <property type="entry name" value="SEN1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase Sen1 N-terminal" evidence="10">
        <location>
            <begin position="83"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase SEN1 beta-barrel" evidence="13">
        <location>
            <begin position="1141"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="11">
        <location>
            <begin position="1298"/>
            <end position="1590"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="12">
        <location>
            <begin position="1598"/>
            <end position="1795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1835"/>
            <end position="2151"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1476"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1856"/>
            <end position="1890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1922"/>
            <end position="1934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1961"/>
            <end position="1970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="2003"/>
            <end position="2028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="2029"/>
            <end position="2042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="2078"/>
            <end position="2091"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF91445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2151" mass="244438" checksum="62FC3B938C4C17DC" modified="2022-05-25" version="1">MSSENSVMEQKYEVAKGFIPTIEKVYAGTNTDIPEANLLGDLLKLLAELPEDFHLFCDPVFAPISVFCLTIFSFNEQSTVSWLKNKFNPTLARCDKCILNFTRGKCKMLQHFAVQRHVPHEHVSKFNDIVCLWRADAVFPVLHKISVNDNTEVKVTREIELAVFECLCNPHMLRLKKDLRMTFDVIFKYLFHAKQPFLDPSSSEFFGKFIAGIIFCWCEGTQEEIQWARIILKKLHEEKYCIGLENFGSDVLEEINIHMIFLQNPANWNEIVVSQFWSRLIPIFTLFEKDIFTEYFEIPKNIDSLKKTVRFPFESIYKMWYNHLGKTYRDKPLEFLLRALCMFLDKLGSAFWTKLEPFTFHSILDMVFDRDIFANKLVRVQNNPIPDDEIGIIMSFSGSITDMVSWTLPFYKALSSSKRVQMVKKVSMAFLRVISNHQSLKSIPKACLMNSSTALLRAVLTIRPDELAMLYDKQDFEVVLYAKTDSRALLNNPLIQDIVMKSATSPTELYPDLGESVVSVSTSAMMVLTKSINFDILMLCEATFKLYAGRHVNELNLTPTLVENLTARLDLRSFHDGPLLAKQLLISFNHINGLLIVEKSKDPAAQRHNALIKQYHHHCTKLVGKFADILPSQLSIILAEREPAQGFWSCVFSSNTELYQAATNILYDTFDVEGRFEGVHAILKNNLTNQLFAIDNVLHQLIKCEFYEPCPRAIRVLMDVVSAFTDPVAGMFSNYESLKNEKTDKELHRFWELTWSFLDTIYRCTLNWATKYEYSELENFTKDTLDLSRSLVDSYREFSDSLNDTKIDLFESVVKAFKNMLYWLRLSDDALLDSCVRLIVSASDLAKEKQIKFDDTLVEMMAKYGSKAKKFSNKLSDQQAREILMKAESFNKQLTDRTASEAEAYHKEKLLAKSGQSSFASAQTSTTVAAESKAEFLQRKATSSSITGRPKATQSKITSFGSFQPSNAIALHQHKPIKPLSKMELARRQLLSNRVIHPPSATVFNHRRTVVKPKDESSSDESGDDIETARELFASAKSKGKGIETVDISGKVIKKGTSAERAKLDGENMRRRLNVDLNPFYEIILQWDYCRCDEYPDDVMKSHCVDLKDQFRSVAEYQQIVKPLLLLECWQGLCSARDRGDFKPFSIIVGNRTAVSDFYEVYASVNKKKLQDSSITESDLIVLASFPNDLPGTEYTNESFKSAGETCLAKVRTLKNTKGDNVDVTLRVHRNHKFSKFLTLRSEIQAVKVMQMTTVEREYTSLEALQYYDLVNQILQATPTPQMEVNNDEIELVKKKYELNLSQAAAIVNTVLKEGFSLIQGPPGTGKTKTILGIIGYFLSTRNNVPANVIKNPGSSSSLSTDQLLKKQKILICAPSNAAVDEIVLRLKKGVCNKEGNIFNPSIVRVGRSDAVNAAIKEFTLEELVDKEVSHKNYEFTKNPELEKKFNEAVHKRRGLRARLDAENGVPTSSMSTEDIAKLQLQIRELSKQLNELGRERDLMRERNSVNYRNRDLDKRNAQARILAKSDIICSTLSGSAHDVLSSLGVMFDTVIIDEACQCTELSSIIPLRYGGRRCIMVGDPNQLPPTVLSGAASSFKYNQSLFVRMEKNMTPYLLNVQYRMHSEISKFPSAEFYNSKLKDGPEVDILNRRPWHEVPYMRPYKFFDIVTGREQQSSKTKSYVNMDEVRVAMELVENLLNKFEKKISFAGKIGVISPYKEQASRMRREFASYFGATVLQEVDFNTIDGFQGQEKEVIIISCVRADDTKSSVGFLKDFRRMNVALTRAKTSMWILGHQNSLVKNRLWNRLITDARERGCLEVACPGFLNPKNSKAQAILARHKPSDDSSLNSDDYDPFESMKPESKVHSKRKHEDWDAQTRRGINNEKKRKETSSSSALQGKKRKSSLFGGPTIANDIGNSGPYIKDKGKPDRKKSENTNPKTKRHVGFSDNVEIIPRIHDQNETPNTKTSTTQRDENKPAQVGSLPLKRSDPESEDDENDYTPRPPEERLPPKRKDEQKKEQNSKVDKSHQSALSAPQTGSGPQNHLYGYQVAIPPPSTHYRPGGNGQQHQLQYYPPTNGFYTENSSYSQMPPTASPVGDSSAAHQSFPGPHQIYKPPSRFNAVRSGTIGARSGDRRQQSSNLFIPKKKQHHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAE6</accession>
    <name>A0A8J2TAE6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ZYBA0S10-04412g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_04412g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the organization of the mitochondrial membranes and the global structure of the mitochondria. Also required for mitochondrial distribution and mobility as well as for the maintenance of mitochondrial DNA nucleoids structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MDM31/MDM32 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316463">
        <property type="protein sequence ID" value="CDF91309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2TAE6"/>
    <dbReference type="OrthoDB" id="17678at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012571">
        <property type="entry name" value="Mdm31/Mdm32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31068">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31068:SF0">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08118">
        <property type="entry name" value="MDM31_MDM32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="91"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF91309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="554" mass="63759" checksum="9ED03FA20A7FEC37" modified="2022-05-25" version="1">MWRTRPSIRGVAGFLRSHGLRPTRSRVSAQRAIRYNSTNQNREDQKPFKFITERDRRLARATSIFEKLKINIKWILTKSTRPFNSDDIGAFISWVLVSNVLIIVLWTTTFASLVIYLMNTVLAQEYVATKVGNFLTKNSALSVVFESAIVPDWSSGKITFNRVFVSRRPKLSHSFTKGSQKEALQRTELALGERLLVSREDFDDGNYTQYDLTIDQVEISLSFSKWLNGRGILDEVSINGLRGVVDRTHVVWLSNSDPRDYKNVHKAGDFEISKFTMSDVLFTLYQPQGFRPIVVSIFNCQLPQLRQHWLFYDLLNANSVSGTYDNSMFTIHRKFNVEKTPFSSSDNSPWKRVTRLRVDNLDIDHLNAGMEGPFGWITEGKVDMIGDVLLPDRCADDRMLTEILTEVGERLFKEAKRRTMAVPQTPAVTEMDPDQYFIMDFFLKLHNVRAEVPLFTPELGYINNALIRPIVGYINSKRTYIPIRCRVVKSIKDFEGSWTIYDSLLMQDLSAQVYDAFTEYVADDREKSVRLKRVTFWSLQIVLQVILMSLGAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAM3</accession>
    <name>A0A8J2TAM3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ZYBA0S11-02146g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_02146g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VAM6/VPS39 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316464">
        <property type="protein sequence ID" value="CDF91406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2TAM3"/>
    <dbReference type="OrthoDB" id="5325112at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032914">
        <property type="entry name" value="Vam6/VPS39/TRAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019452">
        <property type="entry name" value="VPS39/TGF_beta_rcpt-assoc_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894">
        <property type="entry name" value="CNH DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894:SF49">
        <property type="entry name" value="VAM6_VPS39-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10366">
        <property type="entry name" value="Vps39_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vacuolar sorting protein 39/Transforming growth factor beta receptor-associated" evidence="6">
        <location>
            <begin position="595"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="4">
        <location>
            <begin position="717"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1014"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1019"/>
            <end position="1037"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF91406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1037" mass="119826" checksum="A68F61CCA2D07C12" modified="2022-05-25" version="1">MLKANLVDSVASPGVAAVLHNPETHKLVLAKNDQSVEVFSREGNTLRLIQTYPQILRNPRANDATVKELYSCRSLSTIFARCDKSLLLFDSINFHKYDQIVDGRGIEECWVMESHRSHSEETFTTLLYATRNRYKLRMLFWEGRTYKKVVEATLPSSKEHVLSAFASEDGVVLATNHAIYHWPYGESVLVKIDRLIKRKYPSDLIGCLAELASKAHDTTNGKGGLISDTTSFISSSRLTRKSSIASFWTRKSRSSEHSLDSTRLIFKPTNEDQICIFDGYTKNLFHLGLTNSHNPYLLASDLSQFMEWNRGFDTVRYLTSDTLILNNAQNIRIVDYNYGFTFLEQSIPEGIRWVEQIGKSHFIVLTANFQLQLYHYQVEDGTDELLADDESLCGISFDTDFNKLWRKVLFYNHVLNSPYSEKLCESKHPEHSLDLCVLKLRDLTVLWCLESFDRFQNCMSKLEANDSVTEREMVLQDIVMKIIFEKLVEFWAPPQLVVLRTFPPNICRLVPEITGQDHTCLFDDLKTRNTYVIPPLLIQRWVLPYLTDTRRHLKNLAETNTILWKHSGREIDADVNFFLIDKHQSVGVTAMLTLIDTVLFVLYLNYYPSMVGPLMRIDNLCETDLVVEELHGRCMFQELVDFYFQRGMHDNALEFLTNMIINMDQNDNSIKLQDGVKILIIDYLKRLPLENENLLLQYTKWLLQRFGVDTAILESIFMNDLPACVTRDHYEIYKFIDGYNKQVSVRYLEFVISTFGSRDVNLHTTLIELYFKDLSNANTRMKLKSVLESTSVYEPRTILRLLNELLKDEGSSFSQSENEFILFSKIYPLKKLGAHTEVINILYDDLSDYSAASNYCSDIYHDDHELGERSLNYFYKKLITHSQQTKNHSELVYFLQEHSTKLDIINIYELLPKSIPLKDFKETILQTIKSRSISKDENRINKSLLQVELINEGNALNKALSEYAVLDENYRCVVCNKTFSTLTTDTILLFAFNKGDVIVHHNCGKALQAKIQSKKVKAREKSPKRVADLGPEEKMGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAN0</accession>
    <name>A0A8J2TAN0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ZYBA0S11-03972g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_03972g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316464">
        <property type="protein sequence ID" value="CDF91488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2TAN0"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002058">
        <property type="entry name" value="YML081W-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF13">
        <property type="entry name" value="RESPIRATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="103"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="390"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="467"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="510"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="394"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="415"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="467"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="483"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="510"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF91488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1150" mass="130550" checksum="4BAEB548859B3308" modified="2022-05-25" version="1">MTGEKKRSSCLMAWLDHQVCVKDKMSVAQEGAMSRDPRGFEEIPPGVASEREPPALLLPIPKKSRTIKTDKPRPFLCPVCTRGFVRQEHLKRHQRSHTNEKPFLCVFCGRCFARRDLVLRHQHKLHASLVSRTLEADVLAEDQDGTDPMDKNIIKVTGNKESILPTPSNPMAKTTAQLKKAAREASMGNTPTAGSVGDAIGCNSRRKRHASFSASSALSYVNDSSSSQVVREQEPQEGPHQVGFSTPQLTAQELMDKAVESGVVDVGLLGLPPDFNLDDGPNGSLLTNFLTMGSGGGQGGFVNVEAGENSNLTLFQPQDVWQEGVQPAQHEQHQDYQYHHHQQHYPATVDAFIQDPQAAWLASDFISNAHLEPDFKVNFDTFNDVGFADAPSTAGVTPRSNPYNQPPSLSQYQHQHQHQHQHQHQHQSQIQIQNQTQIQNLSQSQQRQQQQQQYQQHNVAENIILSRSPNLSPNSNQSPASNHTQNSMSLGPSVSSLFKNRQKDLFDESYSLLSSPSTKPTPSSPSPPDIRETSSQFRKCTKLFLFNEALRSQIIVANNLTSNLFPTVEELNSYVNLYQYEFHRYFPFVHLHSLVPTIENYPLALSVSMIGALYAFHSSHGMLLSNISWKCVKAYLDNTKDHYESTPLWLIQSMVLLTFLGIFSNDSSVTRSMESRIMTLINLVKITKLNLPLENLRTPPIESDHAFDFQDDPEILAQIRAQYKNPEQIRKNYEYFITAQARIRTCHTVFLISNLFTSLVGLDCSLHSIDLKCGVPCYYEKLYLCENFTEWSVLLAKYRIVLDSKFSLIQLANGYEEYEHSLMYISTGSQYYFENSKVSFKTLLSMLISIYEKIFMERSHLKYEENLQTIEVKWRMTSRPVIESMLKYWEALYIKSGGILTRNKDNIPIINESPSMRLIIPLHLFANIRKCINICPVMNKAWTKDWKGMNEVVDNLCYDMESLREATDYALSIIDFWADSVSLMKNAETTAFRTPIFSITCMFSSILIMAEYMKRVEKWAQDYDSTNLLAPIINASDRLLWLKSEKVLKKVEQHLLPKGYNAQSYSEFLRVQAKGALDVDVLDNDLAQRAVRPDTPMVETAAVIIRARLSTRCLYLGVRILGDAPVWPIALLFAHVLQARAIYNEHNKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAX0</accession>
    <name>A0A8J2TAX0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Origin recognition complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_03422g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">ORC is composed of six subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ORC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316464">
        <property type="protein sequence ID" value="CDF91463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2TAX0"/>
    <dbReference type="OrthoDB" id="1926878at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 1"/>
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    <keyword evidence="3 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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            <end position="186"/>
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            <end position="442"/>
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    </feature>
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            <begin position="445"/>
            <end position="464"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="997" mass="113640" checksum="F52B819680879A4C" modified="2022-05-25" version="1">MAQTLKDIRGWEVITTDENGNIINDNRRRSRKRGGSEKTFLVRLSDGLKFGRGDSVIMDDGGTNTYSVYLIHEIRLNTLNNLVEIWAFSYLRWFELKPQLYYALFNPAFAAENRPIEEYRARFIEEVNKAEIYLTAELSEIWLKDFVDVANIMTESQWKQSGKLEDKDFFVRYICEPTAESYVPIDIVKEKKMLLDAEPKSFEEHLKKMSLPPAVTYNQRPSKAKLNSTRVSGHNKVASLSEIKLETETDDDAEEHEEGRNKAEKEKEKEKRDTNESGEEKGKKTAKSWSQSRIKSRKRKNKSKINTSQTSSPSPPSDAYSDAQETPSTVSKSSPAKGGNKEQPSKVNLFHKLTAEGEDENESGSEQESDSDDAEEDSNFTAKEDDATVDESEDTHEEEDVDSEEEEEIDEEEEEEGEEASNTSPKKVRRKMIPGNHKKSRKLLASSTPATPSRPSTSNSNGSPAIRKFTKRNVARAKKKYTPFSKRFKSVDEIPNLTELAEFNQSTDDLNVAALENKLRTPKKHEIVETIFSKIKKQLYSSHEKEEIVKSTNFQDYLPARENEFASIYLSLYSAIESGSATTVYVAGTPGVGKTLTVREVVKELQRSADQNELPLFQYVEINGLKMVKPTDSYEVLWNKIMGERLTWGAAMESLEFYFNKVPQNKKRPVVVLLDELDALVTKTQDIMYNFFNWTTYENAKLIVIAVANTMDLPERQLGNKVSSRIGFTRIMFTGYTHEELRKIINFRLKGLNNSFFYVDTQNGSARMVTPEEAMQAQLPPHTKRVRLTMSDDAIEIASRKVASVSGDARRALKVCKRAAEIAEQHYMSKHGYAYDGQVSMPEEEDTNDGEELQTVHIAHVTRALNETINSQSTKFIAKMSFTGKLFLYALLNLIKKTELQEQQLGDILDEIHLLIEVNGNNKFILELSRVLFQQDTETSPEQLKIISWDFVINQLVEAGIIFRQSMKNERLTCVKLNISQEDVNKGLEQDENLKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TC20</accession>
    <name>A0A8J2TC20_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol-3-phosphate 5-kinase</fullName>
            <ecNumber evidence="2">2.7.1.150</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Type III PIP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BN860_01156g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57923"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.150"/>
        </reaction>
    </comment>
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    <dbReference type="EMBL" id="HG316466">
        <property type="protein sequence ID" value="CDF91682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="158357at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:fungal-type vacuole membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000285">
        <property type="term" value="F:1-phosphatidylinositol-3-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03334">
        <property type="entry name" value="Fab1_TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PIPKc_PIKfyve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.810.10:FF:000001">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase FAB1"/>
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    </dbReference>
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        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase isoform X1"/>
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    </dbReference>
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        <property type="entry name" value="1-phosphatidylinositol-3-phosphate 5-kinase (Fab1)"/>
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    </dbReference>
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        <property type="entry name" value="1-phosphatidylinositol-3-phosphate 5-kinase (Fab1)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
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        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PIKfyve_PIPKc"/>
    </dbReference>
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        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
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        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
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        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748:SF7">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="15">
        <location>
            <begin position="205"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="PIPK" evidence="16">
        <location>
            <begin position="1752"/>
            <end position="2082"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="54"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="270"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="483"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="600"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1357"/>
            <end position="1480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1555"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1730"/>
            <end position="1792"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="954"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="298"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="509"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1374"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1423"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1454"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1465"/>
            <end position="1480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1555"/>
            <end position="1564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1730"/>
            <end position="1761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1774"/>
            <end position="1788"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CDF91682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2094" mass="237153" checksum="2D1E681A402F5B5A" modified="2022-05-25" version="1">MSGASSDMMSLKQKGPAATVKAAPDKPEFLATQALIPKTTTDLDTNPVLSNISLPQELSNSGSIPLEDTHSDSTANKSRSSGVSTSGLDLEPHTFTHNNHHSGALSPLSKYGRGKSGRSLTFSINNDANIKRSSIYESKSTVTAIPIRNPVTGGKQHHGDNDDASSSKSASSSMTKSFLFGFYDNKKKHENATKGVLSKQYWMKDDSAKECFTCGRAFTTFRRRHHCRICGQIFCSGCTLLISGDRFGYNGKMRVCKNCFEHADNYEDSSDDDSYGEQNSHHNNNNSAIQETSPDKWSSPSAPLSPGLGLANHNPVMKQQDLFLMNDEDVKSIVTTAEDTKLFLSTPPPPPKMAIPATRQGESLEISFPTYRHSNALDSQQHESAGSSGTGVRDRFTIKDVDMLSPYDLQFGSPSQMHGRSSNKNSKKSLRKTVFNYVNNGKHSSERPDSGGSADSIIGNLGSKNFKFEFNYMKSARRSSIESSKKDSGLWLSHAPNDRDETSSSVLLQDDDTSEDEASMSIYSSLNDPMHSRNPIRSMRNSTKSVQRAEASLQRMRFRRKSKSKPTKDSDSIFKGLNIFTHSTPNLVSVVNEDNAFGSQTNTSTNSLAKPKNKSGNAGQWRRISSVSGLRVPREKRNELNEVARLHLKALLDQVIRDQNFETLDFWTPLLKDFLHKVQNIELSARDSNTLDFRQNYVKIKRISGGSIAASEYINGLVFSKALPCKNMPRFLENPRILLIMFPLEYQRNENHFLSIETVFAQEKEYLDKLVSRLVSLSPDIIFVGANVSGYALELLNKLGVVVQFNLKPQIMERIAKLTDADIAISVDKLAANVKMGECEEFKVKTYIYGNISKTYTILRGCNPSLGGTILLRGDSQEVLRKVKDVTEFMVYALFSLKLESSFFNDNFLQLSTSYYVNERKRRREQKYLGYFSAFLEKFNNRILTVSPTVEFPVPFLLERARELEQELLNKKKENELLLARGSMEDDPRIESLGLASKLTQKDLKYLIKFVHEKEMEDLELQFRKRSRHWELSYAQSHNLLGTGSHQCITVLYSMVSTKTATPCIGPQLVTIDYFWDSDISVGQFIENVIATASYPCRQGCGGLLIDHYRSYVHGRGKVDVLIEKFQTRLPKLKDIILTWSYCKKCGTSTPILQMSEKTWNYSFGKYLEVMFWSNNEGVTGIGNCTHDFTKEHVKYFGYNDLVARMEYSALEVHELVTPPRRITWTPYKDIKMKVELYYQILDKINSFYDSVSDRLGRVKLDSMSGDKLAAAQEKLDELKLRVNEEKKTVLDDLEQLYHESPGDQHIKLNVVIKAVHDKVVSWDAEFTEFGKKFLPSENDITRITSNQLKKLFSDFNKSQETKKTGPQAQNKSLEIEDLRFNQQENGKEEERAKSLQQPLGPKNTEETVFDGSKKKKGYHSLSRLNSRQIDPPDNSTSQANQNPLSRPALRKTSSSINSSLDNSTAENVRRSSLNSNFSGDSFFAKEHNTKSKVGELASFFDQMHFDALSKEFEMQRERERIQLNKNKYQASRLHQSTPIVEIYKNVKDAVDEPLHDQSKRASKEGLPNSESHEKISTRASNQNQIDKKLESQLENSIHQWGEKIFHKGGGKKDKVDPEFATNAILNGGKEAITAAAAPPALTIANANKDDANTQPEKSLLMKALTNFWADRSASLWKPLNYPTHPTEHIFVDSDVIIREDEPSSLIAFCLSTSDYRLKMAKLDNQQPTTFARTETHQSSQTPEASFSETESQQSGETLKNNAHKYASMEKDSSITPDDTQAQPQNFQPMPRSENEILESIMTKKTAVHLRYQFEDSLSVMSCKIFFAEHFDAFRRICGCNDRFIQSLSRCVKWDSSGGKSGSGFLKTLDGRFIIKELSHAELDAFIKFAPSYFEYMAQAMFHDLPTALAKAFGFYQIQVKSSISGSKNYKMDVIIMENLFYEKNPTRIFDLKGSMRNRHVEQTGKQNQVLLDENMVEYIYESPIHVREYDKKLLRASLWNDTLFLAKINVMDYSLVVGIDNKGHTLAVGIIDFIRTFTWDKKLENWVKEKSIVGGNSIKKPTVVTPKQYKNRFREAMERYILMVPDPWYQENT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WV40</accession>
    <name>A0A8J2WV40_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA (guanine(10)-N(2))-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.214</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_12332g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM11 methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.214" evidence="9"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WV40"/>
    <dbReference type="OrthoDB" id="296065at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160102">
        <property type="term" value="F:tRNA (guanine(10)-N2)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000260">
        <property type="entry name" value="RNA methylase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016691">
        <property type="entry name" value="TRMT11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059073">
        <property type="entry name" value="TRMT11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370">
        <property type="entry name" value="RNA METHYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370:SF3">
        <property type="entry name" value="TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25904">
        <property type="entry name" value="Tmrp11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017259">
        <property type="entry name" value="tRNA_mtfrase_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00507">
        <property type="entry name" value="N12N6MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51627">
        <property type="entry name" value="SAM_MT_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 10" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA (guanine(10)-N(2))-methyltransferase TRMT11 N-terminal" evidence="12">
        <location>
            <begin position="4"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA large subunit methyltransferase K/L-like methyltransferase" evidence="11">
        <location>
            <begin position="185"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF87694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="435" mass="50017" checksum="5E57AD5A2396ECF2" modified="2022-05-25" version="1">MSPKKYLVYMVPINVNFRRPELESLADLCGLTVDFSDYREDSPFFVVELGNDSQASAWIKRSILTKAIYEYWGEGNDFEELHRSIKSQPFYGLYQRSYLNDTFKFEFEAYRGSSKANRVDKIESFGYLGFQGKIDMKNPQQVYGIIEQYKPISDNVGSTVPEKIYFGRLVQRSDRHAMEVYDLKKRPYKGTTSFEAELSLVSANIAQVKPGSIMYDPFAGTGSFLCAGGHYGALVVGSDIDGRMIRGKGAAQNINSNFKYYQESIQFLDVITMDFTHNSLRENLVIDTILCDPPYGIREGIKVLGAKDPEKYADKEKIEINGKKAYLHRDYIPAKKPYSLDNLLDDLLQWASERLPQGGRLAFWMPTANDDNIETIVPLHSNLELRYNCVQEFNKWSRRLLVYINRGPEFNGQSNSGIKRSTQNFRERYFSNFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WVH7</accession>
    <name>A0A8J2WVH7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_16666g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_16666g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WVH7"/>
    <dbReference type="OrthoDB" id="3992718at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071479">
        <property type="term" value="P:cellular response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026217">
        <property type="entry name" value="Ddc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007268">
        <property type="entry name" value="Rad9/Ddc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15237:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT CONTROL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15237">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02063">
        <property type="entry name" value="DNADAMAGECP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="487"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="556" mass="62718" checksum="490F3AB3E8418A02" modified="2022-05-25" version="1">MSFRASFSSSEKHSIWFRTINALTTVHSDIKFTITSAELIAWAINTTDTTLCQIKFAREFFDEYEFRPYEIVFGENGVQIVPDRDGGDTKLYSFQINGKQLTTISRKPDNDNIKEFIIMVNNTTTCPDTLANRLLVKVEMASLISKEYSPQFIPIKYDPVIINLKYKRKFLNVYGPEAASPTAEASLDPKLLEIFSQAKHELATDLFTDNKDSYPKDKDQLTIDDEINYICCNQALIRNFLDNCNANITEEIKLEINAYKLIITAFTKAIYGRNNDMLRNNMSLSNTISISDIAHYCLFTTIEENDNEVSQHSKRKDATKSIVFKLKDFKNFMTIGSSWKSSLSGNVSMWFCHKGDPLLVEIKKPGIKMELVQITESTGAGVIRDSELVATKIVSPQKRNTRVPPAIEESAAGRISPLKMAPIPSKQVRTSPLKCAGSTGSSLGERPVARQLFVKEETERSLGGWNHGTEATTLHQENCGAPVLAERSRTTVGWGQKPLDPNPPVAQIDKQSMLKKEKRKYLQELQEEQKRQKLSPGKEEEEFGPTQPTRPKGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WVJ8</accession>
    <name>A0A8J2WVJ8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BN860_17392g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_17392g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WVJ8"/>
    <dbReference type="OrthoDB" id="207120at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11887">
        <property type="entry name" value="SH3_Bbc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000316">
        <property type="entry name" value="Myosin tail region-interacting protein MTI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057402">
        <property type="entry name" value="AIM3_BBC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035552">
        <property type="entry name" value="Mti1_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46026">
        <property type="entry name" value="RHO-TYPE GUANINE NUCLEOTIDE EXCHANGE FACTOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46026:SF1">
        <property type="entry name" value="RHO-TYPE GUANINE NUCLEOTIDE EXCHANGE FACTOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25459">
        <property type="entry name" value="AIM3_BBC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="5"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="71"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="195"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="470"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="195"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="227"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="341"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="375"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="483"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="516"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="548"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="606"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="643"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="667"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="690"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="762"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="823"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF87916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1165" mass="127145" checksum="D102D446049FE296" modified="2022-05-25" version="1">MSEPLVPFQVVAQYPYKSEFEDDLNFEKDQVITVTSIEDDQWYFGELRDLDGSIHEGIFPKGFVAVFEGGSTSSTSDVHPTASSMPGSEVQSKPIAVPEPIPESKPAAEPEVKHVASPNRFSMATYDDDEDDDEDHWVDAATSNSPNLKGKMSMFHQDDSQPAPKPRNSVFFDSADAAVKKTVVADSTHHYTPTTLTTHAVPAASAAPAMQTHVEQPGGEEPVNRSNDFKKEDQEDLPKMSLKERIAMLHEQQRRQMQREQEKLTKKAKKEEEAQQLSSSLPVEEEEEYVNKPKGDETYEDDQEPVSLDPSRDLARGVEGLNQDEALQAADMKANASQNLDKAERQREEGGYHDDDEEPLTETPQDYDIMQGKEIEEDDDEDEEEDEEEKRRAALTQKMAKFANAGRYGGPVSFNPFGMPPSMGINTGAELKKKSSVASEEAEPQEEASIPKVVPVMPFADPNAVPFLKKGSTTEEYAEDEIHDTYRDLQDARKSEEAVPLLDTPGDMADEEEAGDSQSAYVENTAFQPAEEESVSPEREQPALVGQTTSTDSTGYDSSDENTDRKISTSADENTRHQPRTSNVPPVPKTPATSSKRSSLLEESDNAQNSFPTISKPSAAEFEKPFTPSDDVSRSPGTPTLDPSVESSQQKSSRSSTSGPPRPPPSRNSSARRTNSSLTRAPSGSLPSAKGPPPPPPVPEAPELPRAAPPIPGTSGLPTPPVPPVPAPGTSNLPSSAIPSVPGSTKPPRAAPPIPGTVEPPRGAPPIPREPPRTAPPAPHAVESSGPPLPPPPIPGTSELPKGSAPPVPGASNSPELPTHPEIPSKHPKTDMKNILPPAPLSPPTAHANAEVRHASNHSTESASTHARGAPSLPTKNHPNTSAPSIPGQPPPAPGVSTADVPKKLNRAASTKEFSDFTNQRSIEFRPQDDWWIDKTFPSGLFGQKVKCVMEVDDHLIKKRSSQKYMVRDFYFLFEDFSQLHVAVSYDVAKPKETVLASQKFYPIKHQPELLNEFSKRYGNFVLQRAESLIGSQTANFLPSILSRLSPEIILPIGSRTFGVPIFRYKAGESSNLENAKNIRPGDVLVIRKAKFDLLTRSGERKTVAVGAETEPYASVITDFEFTKGKFNVIEEYAGKVVQSSYKLHHMRSGRLKVFRITGRQYVGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WVL7</accession>
    <name>A0A8J2WVL7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Inositol-pentakisphosphate 2-kinase</fullName>
            <ecNumber evidence="3 9">2.7.1.158</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_18052g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Has kinase activity and phosphorylates inositol-1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5) to produce 1,2,3,4,5,6-hexakisphosphate (InsP6), also known as phytate.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ATP = 1D-myo-inositol hexakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20313"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.158"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="9">The EXKPK motif is conserved in inositol-pentakisphosphate 2-kinases of both family 1 and 2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IPK1 type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.158" evidence="3 9"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WVL7"/>
    <dbReference type="OrthoDB" id="272370at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035299">
        <property type="term" value="F:inositol-1,3,4,5,6-pentakisphosphate 2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009286">
        <property type="entry name" value="Ins_P5_2-kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14456">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14456:SF2">
        <property type="entry name" value="INOSITOL-PENTAKISPHOSPHATE 2-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06090">
        <property type="entry name" value="Ins_P5_2-kin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF87946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="275" mass="32514" checksum="D426BF3550A2112E" modified="2022-05-25" version="1">MRIVDRGRANLLIDYGNPDWLYRCCIRFPSSLRLCNQYTMDNVRFINEKIRPILLDMLCSMELQTVPLNKLQVLNKHITELDDDIVQLIKIKTLKNESLDHLIYRDHFTSFYCSGDDILMEFKPKWLYSKLGYCRNCTYNSLKNRDISYCYATLLNEPRHFFSIAKQASLPREFVEDIICYFSSSDNILKVLHDAQKKSDVQPWTQIGSVSDVDDQCLLCMTLRDVTCFIRWHPHSSLSVSVVDVDLKPREKWEYWIETHQTLNSYVTTHHSKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WXH1</accession>
    <name>A0A8J2WXH1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_14884g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_14884g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WXH1"/>
    <dbReference type="OrthoDB" id="4070114at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035310128" evidence="2">
        <location>
            <begin position="19"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF88637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="362" mass="36137" checksum="0F76094C5BBC58DD" modified="2022-05-25" version="1">MKLSNVALFMGACSSAVANPVHELLYRRDVANGTTVAGGSNSSAAGDASNGTATASSTSPSSTAATNVTGGGYGNSSSGSGSSSGSSGSVSVFVTGGTSTLSNSSGVDVNWLFNSSQSLNVTQLYQIAIQINQTLGQGSNNGVVIVSNAESLESLGFFSSLVFNTDKPIVIAQNAATGAAIAQDPSSKARGPLVLGDNHLIYPGVFAPSAGETSSCAAVGIASDASNATWFFENSVPALTGPSSVIKQNYTNFTNIDVQNTPVVPIIYDGGYSTQIISSLTSASGFVIVSSGVNSTTSSIGNTSVPVVFAEGGSGLHFVGDEDVPQGTIPAGYLSPIKAQILLSVAAVNGVSSSDALRSLFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WXI0</accession>
    <name>A0A8J2WXI0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_15236g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_15236g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="26401at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000435">
        <property type="entry name" value="Ubiquitin ligase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024990">
        <property type="entry name" value="Apc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048971">
        <property type="entry name" value="Apc1_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12827:SF3">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12827">
        <property type="entry name" value="MEIOTIC CHECKPOINT REGULATOR TSG24 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21282">
        <property type="entry name" value="APC1_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 1 beta-sandwich" evidence="7">
        <location>
            <begin position="1492"/>
            <end position="1576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1370"/>
            <end position="1415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1390"/>
            <end position="1401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF88652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1678" mass="189554" checksum="A9596D4784825B5D" modified="2022-05-25" version="1">MDSFSPFDQRREDNVDSFSKDYYFSDGNERGDIWLCADRKTVQWYVGEQCIRKFTYDQPVIKANFVDFEKAPNCFVIVLRDFAHVYYLSEGDSTTVCFPFPVSNVFWYSQGLVLERKVDQTLCGELDHRFITLSDPMLPFGFLGLSSHQKHTDDFRNVQMLLFPELDTQKITVLYDSLHKQLNFYYTQLLTEHSGAHNDGTISRRDHHSLGSAASFALGSNTGGSSGNSAEAKKNLRKISILNRRATSATLTTSDNSELPSASLKSAESISSSSKVIPRRSLSATLDRMSGALASPTVEFSSNSVQQNHSDVLHQAISTKDVTLTKISSMTLPQHLVSRIKTLKCTALNIHNREAIAIFDPCTHFSKIWMIDLLPDVINSLSFKIYGNSPKDMIRLSNLGTTGSITDMCIYQSNHFPGTLAILHEAGHISLYNPFFELASPQISLPNGCACERLYYISDDRMVLGEKANISANFQLTLFPYPKRASIKLCFEAIKWICSSSVFQAIVYLWQFCLQLQNDTKIGCEFLALESVLTAMILPPASTGTTANSTPPMNEKNSIYQSLHNTSAYIYLSNFGVPYLMPKIIMGLHLIREEMILNVLQRDEVIRLGEFLSFATAIMDWPASWRDYYGSCISTREHPPISGVFAHPLDEPPSVMKSLYSVTENSYAPITPFISYSRLVEMDSRIDHLITPRSFKIIRFYEMTHASDYSNDFLLQVLSKLEITREEISTFPLGILAPLRKMLEIVEEKLCEIDTKLDLSVVSRPDLERCVSTIRQLKHDTEDQIKNLSPYRASLKLCNGNMKMVPKDIYTVLTEVVKSTNRFTLERGMMEENPEMDDFDEGYTLKKNAGLIFSEDRRFYHVLSLLVYYKPRRIQFLPRETEYTKLLRRKKAIARVMSMRTCATGIGFGAVAYATEKPLATQKWIKAPLDFTYIFPDESKVSLEPEELDPARVLWGEFHSGVSSGLRISKKSKGINGSWIAFNKPKELDAQHGGFLLGLGLNGHLKGLEEWHVYNYLSPKKTHVSIGLLLGMSASMRGTMDLKLTKVLSVHIVALLPPGSSDLNINLKVQTAGLIGIGLLYQRSQHRRMSEVLFSQVKSLIIVNEEPISDENYRLAAGLSLGLINLGVGDLIAGENVSANVNALEFSNSFPIGGVGPDEKIINGLLEILKRNHDVEQDWIPENSFVGALMALMLIFLKTGNVAVAESIKSPFKFTPLKVDVRPEIFMYREWAYHMIMWDTIGTDLSFIIPELNHIPDFEVNTDNLPIYYTMAGRVLSVGIKFASTGDQKIRDTLLYLMDRFLPFYQYPGDGKLDFRLAILGINALVNVLSVSASLVMCGTGDLEVFRRTRFLHEIVCGKHSDLFELNQTKKRSDKGKETDSLETEVPSEEPSHYDTVREPETSLNETNIEPEEPLDVTAEADEKADDENQYSKFITTSLSLGFLFLGSGQYALKTSSLDNLSYLILSVLPAYKPDAPLQETKHFWSMAVEPRCLVVRDAETERSISNIPIEITVKLNDSFREEVKELVAPCLLPDVKTIKRLRVKSPLYYPLEIIFDEEVKATEFFRNGTIIYIERRPAKQNKHYNYESISDIQLGLKKRMENLADQSLPGSTSSFADRLFGDFGLTDFTMLELEKEVKSPGNVQNYNLEMLCSDINSGDVTDYQLELWRRRHCGQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WXI9</accession>
    <name>A0A8J2WXI9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thymidylate kinase</fullName>
            <ecNumber evidence="3">2.7.4.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_15632g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="3"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WXI9"/>
    <dbReference type="OrthoDB" id="425602at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:dTMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006227">
        <property type="term" value="P:dUDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01672">
        <property type="entry name" value="TMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000679">
        <property type="entry name" value="Thymidylate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344:SF1">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate kinase-like" evidence="10">
        <location>
            <begin position="9"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF88667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="216" mass="25126" checksum="ACBE00CE285101C8" modified="2022-05-25" version="1">MKRGLLILIEGLDRTGKTTQTTLLFEKLKPNAKLIKFPDRTTCIGQLIDKYLTENDFELPDQSVHLLFSANRWELSQELQRYLLAGKHVVMDRYVYSGIAYSSAKETSGMDLEWCLRPDKGLIKPDLLFFLLNEDPSDTSSRRGFGNERYETLQFQDKVKDQFFNVFRALENYPKPNDRLRVLNVANCSIEKVAQEIWSIVQEKLAKEPPSEFTFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WZL6</accession>
    <name>A0A8J2WZL6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ZYBA0S04-04984g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_04984g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="CDF89467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="2241241at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:siderophore-iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17322">
        <property type="entry name" value="MFS_ARN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Siderophore iron transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000420">
        <property type="entry name" value="Siderophore transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
    </dbReference>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
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        <property type="entry name" value="GLUTATHIONE EXCHANGER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS_1"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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    <feature type="transmembrane region" description="Helical" evidence="9">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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    <feature type="transmembrane region" description="Helical" evidence="9">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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    </feature>
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            <end position="622"/>
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    </feature>
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        <location>
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            <end position="688"/>
        </location>
    </feature>
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            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF89467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="714" mass="81141" checksum="1093D36133BE592B" modified="2022-05-25" version="1">MSTEGDDSFIIDTIKMASELRTREEFVLGHPGDVALDKAEHERMEEELEEEGLQKVNPASPSNAIEITESDGEKQFPEELNEYGLPDRLGASRRLIIRRSELMAAQYDAWPYRLLLLFCAFLCGYGYGLDGNIRYIYTSYVLSSFGEHSLISTINVINAVISAAAQIFYARLSDLFGRLWLFITAIIFYAMGTIIQSQSYDIERYAAGSIFYNLGYVGIILVLLLIMSDFSSLKWRLLYQFVPTWPFIINTWISGNVTSAANPQKNWSWDIAMWTFIFPLSCLPLICCMLHMWYKASKTEEWKLLNEEKTYFQTHGLISYLRELFWRLDSIGLFILAISLGCLLVPLTLAGGVNGKWRNPHLIGPICLGGALIPVLLLYELKWAREPILPWRLIKDRAVWSAMGISFLIDFIFYMAADYLYTVLIVAVDESVESATRISSLSSFVSTVFSPLFSLYITRCTRLKPYIIAGCGIWMLAMGLLYHFRGGKDSHSGIIGGLCVWGVGTTLFTYPVNVSVQSAVSHENMATVTALNYTLYRIGSAVGSAVSGSVWTQTLYRDLLKRISDPKIAALAYESPYDFVAAYAWGTPYRDAAVESYRYVQRLLTIIAIVFCAPLMFFSLCLRNSKLTDEIAHENLKEGEVIDREEEDPVFEWMMRHARKVIPGLRNKEPEVTSEEGPDIESDSVEQKTESEFKKLKSRFRWKKSPEQTEEITY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X034</accession>
    <name>A0A8J2X034_ZYGB2</name>
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        <submittedName>
            <fullName evidence="13">ZYBA0S04-10088g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_10088g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="26740at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="MMM1_dom"/>
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        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466:SF19">
        <property type="entry name" value="NUCLEUS-VACUOLE JUNCTION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TEX2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="11">
        <location>
            <begin position="92"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="12">
        <location>
            <begin position="263"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="477"/>
            <end position="814"/>
        </location>
    </feature>
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        <location>
            <begin position="478"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
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            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="544"/>
            <end position="554"/>
        </location>
    </feature>
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        <location>
            <begin position="560"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="592"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="619"/>
            <end position="629"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="686"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="711"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="752"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="762"/>
            <end position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF89681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="814" mass="91753" checksum="1630CE158314D0FF" modified="2022-05-25" version="1">MISFKLFLAVYLLGGVTFIPLLVITLLYANKWSERLRTRKSTGFDEQLLLTGLDPEFKAGEIEEPKGVKVYRKGWLTVTKKYYYHHSEVTQMVEKEGQDVDVPQRSQLKKRHKFFAVLKHGNLFLYRDDSQKSNLIHAISLQGSFVTIWPRDPKHEAPDASMFTKKTCISILRDGTVNYDGSLHFETRPEGQENSSWSQFYLYFENNIEKEDWYFALINVSKRDPKDQTKPSSVIDPNNCARTAHLKTADSLYLIQAIYSTEGQLSAKWLNALLGRLFLSLQRTDALKNYIYGRLYKKLTKLNKPGFLDDFVIEEVDVGTSAPMITNPQLLELAPTGLTRISLNMQYRGNVSVIVATNVTINLGSHFRQRQVPVQLAIKLNELRGPMFLMIKPPPSNRLWYTFQSEPILDVDIEPVVSSSKFSYGMVTNAIKSKLKEAIKESLVVPFWDDFAFYNTEDEIYRAGIWEKFNEKVNPSGFEDHGHTTEDLVGHKDNKATSEKSYDQADNKVSSENSDDRSEENSTSSDESSISERPQALKPRSMSKHSGSISSGGVHRSKSSDSIKTQDSNLKTRTLQRVEHFKKAFSTKNKDDSDDEQTLDDDDNSGDEDPKDSSDSKKYFKNSLKKFGKWYRGSSDNESEEASHGRSSGSRASSYMGKPANNHPPEMISNRRKTLPRRPVPPPLKSPTSPSSVSSASFSPTLNATEMFANKSRSLSNMSSETNDSVVSPTLGSHNHAAFVKPRRAPGFSEELFADGMEDPDISTTPTNQEGGRNTNERFGPPEAPSRPELSSEPLQNHDSRVHSSNVTESDRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X0R5</accession>
    <name>A0A8J2X0R5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ZYBA0S05-04324g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_04324g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF89899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X0R5"/>
    <dbReference type="OrthoDB" id="27962at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058567">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058568">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058564">
        <property type="entry name" value="TPR_TRAPPC9_Trs120"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013935">
        <property type="entry name" value="Trs120_TRAPPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058563">
        <property type="entry name" value="Trs120_TRAPPC9_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512:SF5">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26280">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26282">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26283">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26251">
        <property type="entry name" value="TPR_TRAPPC9-Trs120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08626">
        <property type="entry name" value="TRAPPC9-Trs120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trs120/TRAPPC9 N-terminal" evidence="4">
        <location>
            <begin position="8"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 TPR region" evidence="5">
        <location>
            <begin position="365"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 third Ig-like" evidence="6">
        <location>
            <begin position="964"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 fourth Ig-like" evidence="7">
        <location>
            <begin position="1158"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="338"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF26282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF26283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF89899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1260" mass="144227" checksum="3356569419A0B4E7" modified="2022-05-25" version="1">MNPLTYDASFVQPCRVRLLVVPIGKWNRQKFLDAVMKLRKYNEVRLLDITPIDSPLFTPQGFPNGRLFFEIDTSGHHDALNLFLYDFEPFRKIFVVIGLVNDNSEVTTNLKVLKEKFPTVISHNLIYVGEKKTGLENDVHAFYNGLDYELNLETIICDIGRNFLNALSHYYSSYKHVTLRSPGAIGGNSVLKTAFTRQLVGANALVNSSSTSSKRNTFESTTGSIKRSASLKLAKSLSASENKSQVRSQGRQCKILGNFQLLAGRYIDALNSFTEAVTHLYKVRDHLWLASALDGIAICFLLLTFLKVSFQIPSIVMLIVPVQTSSNSMEINSSRSNATPPNKSPRNSSSTLALGQDFDVENVNLPIFIKMISEKILYYYELSLNHHCDYAPQIVYAETLLKTLTFMVACRSSSRLSQPVLEKIIKGTSLKTHEGEVDDNEQDDSSMIPNEQEEVLFTKLEIYSFANKTFELQLKEMEIESQSNVYILLSKVYHSLGFTRKRLFVLRLLLVALLANQDPMSWYSDYQEILDGMVKLYGIEDRKPESSVEDSQAMAWLTLQKKCLHLCLMVARKLKCSESAARYAVMLVSRYTHLLTQSEQQNLFVAYIQPYFQVGYIKSYWDPFLLRDVKLARLESDISGGELPLESEVAVLTDKPNSGTALIHPEEIFNPFRHLLSADKIVAENSPATLQNVFLVGDRAMFSCMVQNPYKFEISITGVQFNSSATKFCELSKNDVSLKRPFYVSPESIRIINLPMELKSPTHHDWLDIDSVQLSVMGMPMGDFPIALSENHIPRAGLPNDRFNAGKVQIKVLPEQPELQLLRTDNMTDNSWMMLHGTKKRFTVTLRNKSLKCPIEYLQFLHATNVEQSVKPDYWKKMQLDDLHGLETQLEWLKNSCIKVLNPPQRMEPNETCFFELEVDASAMPFHFNGFDLVINYGMNANDKSRVYLKRLQSTYNVTLKRSIEVPSIEIVPLNENFASEVSHVDWVHYLLKRQREDSQFDVSKYLLLLLDMRNSWIDSIKIQIQYEDFVSHEHVIETNHTTRVVVPIRKLEYQNYNFKKKLIPRVWQGRQYIQSGLTDEQEIDMREKFWCREHILSRLRCNWTLSTDDSVKGMVNFRQFVEKFDTRMILSLYEAKSPYHIDLSIDKRSVAKGQSLNAVVKVSPTSILDPALQSPPEMLFLSFMIFDNHTARLLTKSDKRVLFNGTLSRCLRVTKPVETALQLLPIEKGSYEICACISRSNTSDQVLQFNPGLVTFTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X4Z0</accession>
    <name>A0A8J2X4Z0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Plasma membrane ATPase</fullName>
            <ecNumber evidence="17">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">BN860_08394g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Cell membrane</location>
            <topology evidence="2 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="17"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X4Z0"/>
    <dbReference type="OrthoDB" id="116380at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000011">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000005">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000008">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 17" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="14 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 17" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="335"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="698"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="725"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="761"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="831"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="864"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="75"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="16"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CDF87526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="924" mass="100992" checksum="9DA3AAEB9AAFDDE0" modified="2022-05-25" version="1">MSDERITEKHPHQQSDSEEEEDVPQSQSEEEVEEETEEEVTDESSSEDDIDALIDELQSNPGEDEEEEDDGPAAAGEARKIPEELLQTDPSYGLTEEEVVHRRKKYGLNQMSEESENLFVKFLMFFIGPIQFVMEAAAILAAGLEDWVDFGVICGLLMLNAGVGFIQEYQAGSIVDELKKTLANSAMVIRDGQLQEIPVNEVVPGEIMQLEDGTVISADGRLVTEDCFLQVDQSSITGESLAVDKHYGDTVFSSSTVKRGEGFMIVTATGDNTFVGRAASLVGSASGGQGHFTEVLNGIGIILLVLVIITLLLIWTACFYRTDRIVPILRYTLGITIVGVPVGLPAVVTTTMAVGAAYLAKKQAIVQKLSAIESLAGVEILCSDKTGTLTKNKLSLHEPYTVDGVSDDDLMLTACLAASRKRKGLDAIDKAFLKSLINYPKAKNALTKYKVLEFHPFDPVSKKVTAIVESPEGERIICVKGAPLFVLKTVEEDHPIPEDVHENYENKVAELASRGFRALGVARKRGEGHWEILGVMPCMDPPRDDTAATIAEAKHLGLRVKMLTGDAVGIAKETCRQLGLGTNIYNAERLGLGGGSTMPGSELFDFVENADGFAEVFPQHKYAVVDILQKRGYLVAMTGDGVNDAPSLKKADTGIAVEGATDAARSAADIVFLAPGLHAIIDALKTSRQIFHRMYSYVVYRIALSLHLEIFLGLWIAILNHSLDIDLIVFIAIFADVATLAIAYDNAPYSPKPVKWNLPRLWGMSIIMGCILAVGTWITLTTMFLPRGGIIQNFGSIDGVLFLEISLTENWLIFVTRAAGPFWSSIPSWQLAGAVAAVDVIATMFTLFGWWSQNWSDMVTVVRVYIWSIGVFCVLGGAYYLMSESVAFDRLMNGKPMKENKSSRSVEDFLAAMQRVSTQHEKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X563</accession>
    <name>A0A8J2X563_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alcohol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_10616g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X563"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000947">
        <property type="term" value="P:amino acid catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08297">
        <property type="entry name" value="CAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000002">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="10">
        <location>
            <begin position="19"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF87621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="350" mass="36808" checksum="9EFD5D72AC6C05EB" modified="2022-05-25" version="1">MSPVIPETQKGIIFYEPHGKLHYKDIPVPKPKPNELLINVKYSGVCHTDLHAWHGDWPLPVKLPLVGGHEGAGVVVGMGSSVKGWKIGDLAGIKWLNGSCMSCEECELGNESNCPEADLSGYTHDGSFQQYATADAVQAAKIPKGTDLAEVAPVLCAGITVYKALKTSEAKPGQWVAISGAAGGLGSLAVQFAKAMGLRVVGIDGGPGKEELFRSLGGEVFIDFTKEKDIVGAVIKATNGGAHGVINVSVSDAAINASTKYVRANGTSVLVGMPAGAVCKSEVFHQVVKSTKIAGSYVGNRADTREALDFFSRGLVHAPIKIAPLSTVPEIFEKMEKGQIVGRYVVDTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5A1</accession>
    <name>A0A8J2X5A1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ZYBA0S14-00936g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_00936g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the formin homology family. BNI1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316467">
        <property type="protein sequence ID" value="CDF91780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1104827at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:actin binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0005522">
        <property type="term" value="F:profilin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070649">
        <property type="term" value="P:formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Cytokinesis protein sepA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cytokinesis sepA protein"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Formin, FH3 diaphanous domain"/>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Actin_filament_regulator"/>
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        <property type="entry name" value="ARM-like"/>
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    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
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        <property type="entry name" value="DAD_dom"/>
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        <property type="entry name" value="FH2_Formin"/>
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        <property type="entry name" value="FH2_Formin_sf"/>
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        <property type="entry name" value="GBD/FH3_dom"/>
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        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47102">
        <property type="entry name" value="PROTEIN BNI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47102:SF2">
        <property type="entry name" value="PROTEIN BNI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00498">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="6">
        <location>
            <begin position="212"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="7">
        <location>
            <begin position="1319"/>
            <end position="1737"/>
        </location>
    </feature>
    <feature type="domain" description="DAD" evidence="5">
        <location>
            <begin position="1760"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="4">
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    <feature type="region of interest" description="Disordered" evidence="4">
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            <begin position="318"/>
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    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1109"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1168"/>
            <end position="1303"/>
        </location>
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        <location>
            <begin position="1715"/>
            <end position="1764"/>
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        <location>
            <begin position="1829"/>
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    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1955"/>
            <end position="2003"/>
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        <location>
            <begin position="733"/>
            <end position="803"/>
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        <location>
            <begin position="1"/>
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        <location>
            <begin position="36"/>
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        <location>
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            <begin position="1993"/>
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        </source>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51231"/>
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    </evidence>
    <evidence type="ECO:0000259" key="6">
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CDF91780.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2003" mass="226318" checksum="D80C53842E3B755E" modified="2022-05-25" version="1">MIRSSGSRNPNSHHNSNPSTGSSGSSSIIQGLKRLANSQSNSSPPHQRIETGDISSPKQVSVPSRVSNASDLLPLQHSRTPSQQSQSKYTYSRRASAVSQQQQYQQQQYQQQLQQQQQQQQYQQQSQFQQQPQYQQQSQYQQLQHQQLQHQHQQQYQHQRQASLGPTGGPISRQPTNNSHATSQSSAASILSQGSLSNLSKFMRPDGTIRLEMPKDRNEIEALFEDILYKRNIVQTLSPEKQQVLRNYDIEKKWLIVKQDLQTDLKKMISKNSVASSHVSVDSTVLSPTTLTTSGAAHEMVDSSAAVGVSGMKVPMKSSASSKTLNSSSSSVSTQQVSINPPSTASSTEKTNRPPIYYVKKIIADELSTDETNDLWVTLRTEQLDWVDAFLEHQGHIAMANVLMKSLYKVSQDQRLTDRALERETSYFKCFRVLVMLAQGLYEFTKHSIMAETVAYALFSIRLPTRKMATEIFVCMLEKKNKMRFEVILSALDRKFMIGENLHMMQYVGNSPQNFVHLKRDSQLKIVQAWLTSIESTLQGRGKMGSLVGASEEFRAPGGENSILEYSQWTMVFINHLCMGTDVINQRVLLRTRLENSGALRIMNRFKLLDYDKITAQVEYYENGKLDDINSLLETEGQNSQVNMQDPMMMLQKMWGYCKGTENEKTFTSLVQHLFLSSSKVAEDKNDPSKLAKQLKLMDSLVANVSISTVDEESSMNLAIQRLYDAMQTDEVARRAILESRNLTRELEEIQAERDFLRQKLNSTGDGIVGRLEEELRQRDDILAKNQRVTRHLQSELQELKKKHLLEKHEHEVELRKMLTILNARPDDDSLAKSARFSKPGMDSEKQNSIQKALQDGLQRTRKDLSVDSKRFGITVQPNKRLRMLRMKMEGIEQEARELEMTNFSDHEKKQLQEPIQIKRDDSKKQASVRKLSYLRKQLTDIQKETNDISKFNVEERVREMFNEKRLQALKKLKELETTYKGFGIDFNPDELLESKGVDSSLYEKEIDTQDLKAAQSKMDEMDRITREMAVLKANLENQQKLQQMQQQQQQQQQQQQQQNKQQSDSSSTESSSEDDEDSTEVRNDTASEFSQRSFGSGAGSFLEALSQKYGSGQNSGSSTSPTQGNFGVVRNNSNHRTSFANRMKKSGNSTPYFDELTRKVARAPSLTLSNAASGDEDTLPAASPRIPQSIDDTLPRDKVSSSSPVTSAPPPPPPPPLPDILSGNAGTMSKTTEGEANAGPAPPPPPPPPPPPPLPSFGKASLKGSSSVPAPPLPPGLDTSGLDSSSPSPPPPPPLFPGQKKMYRSDMPDLLEETTTFRDNYPQPHKKLKQLHWEKLDATEDSIWSSGVAEKFAGDLYEKGVLAGLEKAFAAREIKSLASRKKDDLAKVSFLTRDISQQFGINLHMYSQMTVDELVIKILKCDRDFLQTPSVIEFLSKSDITEVSINLARNFSPYSTDWDGIRSIEDAKPPEKDPNDLQRADQIYLQLIFNLQSYWSSRMRALKVITTYDKEYQELVSKLRKVDKAVGAVLKSENLSNVFNVILAVGNFMNDTAKRAQGFKLSTLQRLTFIKDSTNSMTFLNYVEKVIRDNYPSFNDFLKELEPVLDVTKISIDQLVNDCRDYSQAVINVERSIEIGNLSDSSKFHPLDKVLIKVLPILPEARKKCDLLDDEVRLSIMEFENLMQKYGEDSGDKFAKNTFFKKFADFVQEYKKAQSQNMKVEEEERVYERHKRMVEEQQKREQTPPRTTSGDGDDDDGDDDRDRRDVMDKLLDQLKNAGPTNSDPSSARKRAVIRKKLLADKDAAVHLISDLESGEDSIVYSPTSPIEETMHSNAESPTLHKSQSSILNSQTPDKGSPTKKLGSQDTEDEEEISDKAKILLMELSGNGTPTKRSEALNGHKEKLRARRRRTHVRNESTSSNKLYFLEDETPPITPVEVEFNKENINEQLRQQCLPDDSPQEIRGMNNATRVITSSSERDGEYDNESAKEETKGFSSISESSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5F9</accession>
    <name>A0A8J2X5F9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL3m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_14994g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5F9"/>
    <dbReference type="OrthoDB" id="274683at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="50S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.810:FF:000001">
        <property type="entry name" value="50S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019927">
        <property type="entry name" value="Ribosomal_uL3_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03625">
        <property type="entry name" value="L3_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF87810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="273" mass="29827" checksum="E5508AFE480AA49D" modified="2022-05-25" version="1">MSSVMWSSICKPVTFARCSSTRAHLIAPSVVSTIKLEAQPINHSPQQAQKRKWLPLRCGALSKKEGMMPFFDESTGRRVACTVLKLDNVEVLMHRTVDENGYFACQVGYGSKHPAKISRQMLGHFASKLVNPKEKVCEFRVKNEEGLLPVGTVIKPSFFQNGQFVDLRSTCKGKGFAGVMKKYHFAGLRASHGTSIMHRHGGSYGQNQDPGRILPGKKMPGHMGNHQVTIQNAQVMSVDDELGVILVKGSIAGAPGSFVKIQDAIKKPPATGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5M6</accession>
    <name>A0A8J2X5M6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BN860_18954g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_18954g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5M6"/>
    <dbReference type="OrthoDB" id="1532798at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF6">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="4">
        <location>
            <begin position="209"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="58"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="109"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="154"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="402"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="81"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="406"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF87983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="452" mass="50300" checksum="A230D8484046206F" modified="2022-05-25" version="1">MVSMDDQQARPAPETRPVSILNSKPQAVRKDTEDKKLLSHFNNLGLGTRDHVKSQVGALQGATPGSCGKPHGNLRVKTDGSVESSPQKQSGSGSAVSFTLSDAQKISQHRYSINSKPAKPETASQESLTEEQDSPLGGKNSIVHMPGDFIYFDSNSKPSAASNGANATQGVPGSHTLSMPPRKEVIGPNAGPQVPFTEFFQKQDDKKFHILLGATGSVATIKVPHIIDKLFKIYTREKVSIQLIVTKPAEHFLRGLKISTDVKIWREEDEWSGFRKMGDPVLHHELKRWADVFLIAPLSANTLAKLAYGICDNLLTSVLRDWTPATPVLVAPAMNTFMYINPMTKRHLSILQEDAPYVSVLKPVEKVLICGDIGMGGMREWNDIVEILRRRISEICRARDENDVVLQDEEEEEEEEEDEDDEDEEKEDQEDDDDDEDEDDDEDDEDDDEAIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5Q8</accession>
    <name>A0A8J2X5Q8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_18272g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5Q8"/>
    <dbReference type="OrthoDB" id="10264220at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF59">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A, GOLGI ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="464"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="509"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="586"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="618"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="679"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="816"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="116"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF87955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="877" mass="100684" checksum="1D1B019CA2ACA92F" modified="2022-05-25" version="1">MVGEEAIFRSADMTYVQLYVPLEISREVVCLLGNLGSLMFRDLNKDLTAFQRGYVSQIRKLDDVERLVVYMQEVNEKHAEATWKYVLHTDEQGNDIERPSLTQLVSEMQTHSHDSINAALDEIHEFESRVRRLDDSLSDLKGRLNDLLERRYVIFEVTRFLQSHPGLFGRLEREQRGLGDVDDFQLDLDDISETLSATFSFDNEEEHNDRGRENPHDAVSNGDNASDQNLLEMGFHDRFMVAGSIRRDKIEVLNRILWRLLRGNLFFQNFAIEEPLLEDNEKVEKDCFLVFTHGETLLQKVQRVVDSLNGKVVSLAHCTHREIQNLNDKISEIQQIAHTTESTLHTELLVVSDQLPVWNAMVKREKYIYATLNLFKQETHGLVAEGWLPSSELPLVSDTMKDYSETVGSEYSTVVSVIHTNRAPPTYHRTNKFTQAFQSICDAYGTATYKEVNPGLATIVTFPFMFAIMFGDLGHGFILLIIGIYLLLSEKKFESMQRGEIFDIIFTGRYVIALMGGFSVYTGLLYNDIFSKSMTFFNSGWKWPSNFKEGENIMAKSVGVYPFGLDYAWHGTDNGLIFSNSYKMKLSVIMGFIHMSYSYMFSLVNYRYRNSPVDIMGNFIPGLIFMQSIFGYLTWAIIFKWSKDWIKDEKPAPSLLNMLINMFLSPGHIEDQLYPGQRFIQIFLLLAALVCVPWLLFYKPLMLRKQHSGVRLDGYDSIRNGERVDELSTDLGATDGEGMVVTDYNEADENEEFNFSEVMIHQVIHTIEFCLNCISHTASYLRLWALSLAHAQLSSVLWEMTIKNAFSPKNSGSPLAVARVVCLFAMWFVLTVCILVLMEGTSAMLHSLRLHWVEAMSKFFEGDGYPYEPFTFKNIME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X672</accession>
    <name>A0A8J2X672_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_12552g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_12552g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X672"/>
    <dbReference type="OrthoDB" id="29879at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010961">
        <property type="term" value="P:intracellular magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12829">
        <property type="entry name" value="Alr1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000008">
        <property type="entry name" value="CorA family metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.20">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044089">
        <property type="entry name" value="Alr1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21535">
        <property type="entry name" value="MAGNESIUM AND COBALT TRANSPORT PROTEIN/MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21535:SF55">
        <property type="entry name" value="MAGNESIUM TRANSPORTER ALR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="568"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="599"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="204"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="649"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="659"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="697" mass="78237" checksum="04F1DB311FA97B82" modified="2022-05-25" version="1">MSEESNSRGSGSSSQLAPTDSLVSSLVSMDTEEHHELYDHRQQPEHLPVVAGAPSVSSNVEVCSKPPRSRANSRVSSNAPMGKTVSHVATKQQRRASFDTDNSQASRESQETEEDVCFPMHPQLHTRINGIDFDEMEEFARDSQQTNQYLMTQNRDLQNVLEGSEPVFNTGGSSSSTFTASSAALKYIPKTAIRPAINTGQFSTNQVPGISFGSNKVEEDESDDKKPELPQQWQPREELYEPQFVAPDRYSFFSSESEETVHATSIAGLVKPGQHFAQLFRNGEPTWWLDCSCPTDDEMRCITKAFGIHPLTAEDIRMQETREKVELFKSYYFVCFHTFESDHESEDFLEPINVYIVVFRSGVLTFHFGPISHCANVRRRVRQLRDYVNVNSDWLCYALIDDITDSFAPVIQSLEYEADAIEDSVFMARDLDFSRMLQRIGESRRKTMTLMRLLSGKADVIKMFAKRCQDEANGIGPALTSQINIANLQSGRDDFPNSNKPGQFPNTTQPRGDIALYLGDIQDHLLTMFQNLSAYEKIFSRSHSNYLAQLQVESFNSNNKVTEMLGKVTMIGTMLVPLNLITGLFGMNVKVPGQDANNLGWWFGILGVIILLGISAWLSASFWIKRIDPPKTLNDAAESGAKSVISSFMPNNARSNRNKRYESSGDRRSSTNLFSNRFGPSANRSVASLPSKYSRYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6E3</accession>
    <name>A0A8J2X6E3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Squalene monooxygenase</fullName>
            <ecNumber evidence="4 10">1.14.14.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_07976g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, and is considered to be a rate-limiting enzyme in steroid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>squalene + reduced [NADPH--hemoprotein reductase] + O2 = (S)-2,3-epoxysqualene + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25282"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:15441"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the squalene monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.17" evidence="4 10"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6E3"/>
    <dbReference type="OrthoDB" id="1678617at2759"/>
    <dbReference type="UniPathway" id="UPA00767">
        <property type="reaction ID" value="UER00752"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004506">
        <property type="term" value="F:squalene monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013698">
        <property type="entry name" value="Squalene_epoxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040125">
        <property type="entry name" value="Squalene_monox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835:SF0">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08491">
        <property type="entry name" value="SE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 10" id="KW-0274">FAD</keyword>
    <keyword evidence="5 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0492">Microsome</keyword>
    <keyword evidence="8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene epoxidase" evidence="11">
        <location>
            <begin position="197"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF88343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="492" mass="54429" checksum="C91B087448BA0130" modified="2022-05-25" version="1">MVATELLNADSGVIYDAIVIGAGVIGPAVATGLARKGKKVLIVERDWSMPDRIVGELLQPGGLRALRSLGMVQAINHIDAYPVTGYTVFYNGEHVDIPYPYKSSCPPVEKLKDLVKDGNDKVVEDETIHIKEYEEDERERGVAFVHGKFLNNLRSIVGQEKNITRLQGNCVEILRNAENEVVGAKVNIEGRGKVDFKAHLTIICDGIFSRFRRELDSQHVPSVSSSFVGMALKNAKNPAPMHGHVILGTNHMPVLVYQISPDETRILCAYNSPKLPTDIKSWLIKDVQPFLPESLRPSFDEALSDGKFRSMPNSYLPARQNDVTGLCVIGDALNMRHPLTGGGMTVGLNDVVLLIKNIGDLDFSDRETVLDELLGYHYERKNYDSVINVLSVALYSLFAADNDNLKKLQKGCFKYFQRGGDCVSVPVAFLSGVLPRPLLLTRVFSAVALYAVYLNIEERGFLGLPMALLEGIMILFTAAKVFTPFLYRELCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6G3</accession>
    <name>A0A8J2X6G3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_16512g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_16512g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6G3"/>
    <dbReference type="OrthoDB" id="79687at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14011">
        <property type="entry name" value="PK_SCY1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF6">
        <property type="entry name" value="SCY1-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="638"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="722"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="652"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="729"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="754"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="780" mass="88258" checksum="54818E38280D5803" modified="2022-05-25" version="1">MFWFGKTGISSKYSFSASPTFVTEPWSVYTGRPKDGNSTGVSKVSIFIFDKKRFEAHLLNYGIIKSRNSSHDRTLIAEAYDILKKQVSNLAKLKHPNILQLVEPLEEHSKNFMFVTEYVTGSLESVFTNVDEELNFFKGHVREDIVIQRGILEITQALDFIHNRASCVHMDVQPRSVFINDNSDWKVSGLGHLWSLPKGTNSAEYFMHQYDPRVPPFMHLELDYTAPEVVLDSTVSFKSDYFSLGSLILKLYTGKSFLSSENSSTQYKDEYAKFERRLAGRSWDGVLADLPRNLKVCMPKLLNRDLYERYDNISDFLQCDFFQDPLMKTLNYLDDLPTKDKEDRLLYLEGLVELLPKYPTTLLQRKFLTVLLHLLGQLCGDVELDSASISMDVHLVIRIGSTLSQLTFQERVLPVLADKSTFPHILHCATLPLVENLSTLKEKIKQDDFVQVILKPLLTYCFHEMKGELSVTVQEKLLEKMTFVTDVFDFLTVKSFLLPLLSELFSKTTSLTVKNACVSCFQLLIERKVVDSDLVCQDIIPLFQSMKTRDPRILMQSSKFFEIVPQIVKNEKMLVEQLLPLLWRYSLSPTLQTAQYSQYVQIINKLSSDLQKKHMEGLQKTDHSHEAKSARDFNKLIEPTSTGAPAEDADTKNANNISTPAIQPVRKNTPVAASRPATKVSNARTARFTEELPKRSQLTRPPRRPEINTELAADIDDFDEFVSASSPTSSQRAPSGSAGPPPSLNSLLAAQPPISNGNQTATKLNSLPPGFSLPLQPSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6H6</accession>
    <name>A0A8J2X6H6_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">coproporphyrinogen oxidase</fullName>
            <ecNumber evidence="4">1.3.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_17282g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aerobic coproporphyrinogen-III oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.3" evidence="4"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6H6"/>
    <dbReference type="OrthoDB" id="15318at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00322"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004109">
        <property type="term" value="F:coproporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1500.10:FF:000006">
        <property type="entry name" value="Coproporphyrinogen III oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1500.10">
        <property type="entry name" value="Coproporphyrinogen III oxidase, aerobic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001260">
        <property type="entry name" value="Coprogen_oxidase_aer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036406">
        <property type="entry name" value="Coprogen_oxidase_aer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018375">
        <property type="entry name" value="Coprogen_oxidase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003727">
        <property type="entry name" value="PRK05330.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10755">
        <property type="entry name" value="COPROPORPHYRINOGEN III OXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10755:SF0">
        <property type="entry name" value="OXYGEN-DEPENDENT COPROPORPHYRINOGEN-III OXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01218">
        <property type="entry name" value="Coprogen_oxidas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000166">
        <property type="entry name" value="Coproporphyri_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00073">
        <property type="entry name" value="COPRGNOXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102886">
        <property type="entry name" value="Coproporphyrinogen III oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01021">
        <property type="entry name" value="COPROGEN_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF87911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="314" mass="36231" checksum="B4EBC30C48DF67F8" modified="2022-05-25" version="1">MAPQIRQEMEALVRRKQAEITQALEKLDNVKFHTDTWLRGNNGGGGTSMVIQNGETFEKGGVNVSVVYGKLSPAAVSAMRSDHKNLELTHSEGTKFFACGLSMVIHPHNPHAPTTHLNYRYFETWNADGTPQAWWFGGGADLTPSYLYEEDATHFHNLHKQALDKHGKDLYPRFKKWCDEYFYIAHREEMRGIGGIFFDDFDERSPEDALKMIEDCFDAFIPAYIPLVARRKDMPYTPEEKKWQAIRRGRYVEFNLIYDRGTQFGLRTPGSRVESILMSLPEHASWYYDNHPEPGSREQKLLDATQHPREWATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6K0</accession>
    <name>A0A8J2X6K0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_18294g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_18294g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6K0"/>
    <dbReference type="OrthoDB" id="10262720at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034663">
        <property type="term" value="C:endoplasmic reticulum chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10230">
        <property type="entry name" value="ASKHA_NBD_HSP70_HYOU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF3">
        <property type="entry name" value="HYPOXIA UP-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035211559" evidence="7">
        <location>
            <begin position="23"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="551"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="829"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="835"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="865"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="880"/>
            <end position="896"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="896" mass="100953" checksum="78E14A08CE1E960D" modified="2022-05-25" version="1">MKFPVMFLLLSTWCLFWNATLAAVIGIDYGQQFLKAMVVSPKAPMELILTPEAKRKDVSGLAIKTLDGKGAEIERIYGSAVGSMATRFPKNTLLHIKPLLGKTEKDEREISLYMREHPGVNIVPTERNSLAFLVDNVQYPVEEITAMNLQEMINRANSLLRDKDPNGIDVVDKLAITVPEFFDQYQREALLDSAHITKNGLETVLINDGLSVMMNFALKKRDFTGKSYYIVFDMGGGSTKASLFSIERPENDSEGLRIEFGGYGYNPHLGGSKFTLDVANLIENKFLANHKKIRTEELHRNPKTLAKFVQAAEKAKLILSANSEASISIESVINDIDLRTKITKEELEEFNEDTLSDIVMPIKDALTNQLWEDQVQLKQLAGVILTGGSSRVPLVQHEVNKFVGESKVLKNVNADEAAVDGATIRGVKLFEAFRTKPLNVTERSISDYSMKRDDKKTVVFPKGTIFPASKTMTIGNAEELKEDLLIDLYEESTLFESVKTKTLVADKIYSPEKCPEGIIYNATFSLSQNRLFGIDKIEAICSKGLSISGGNSKNDTTKADQQRKTNLGGSKRPLKLTTSSENTVIKAMSLEEQLKLREHIKVLDEKDTARFELEEAKNLLEGLLYETRFFLEMEEVAEKGPQTLIAKLARAVTDHLTWLEDETDNATKEDVILKSLEVQQLKDKIDAYLQSLSEPLDSQQFETLLDSANELLARFTLAQGNHTEHLEPLRDNFTDLGYDIHEQYSKISLPHHIAAPLKDWNDTLSTFKDAVTLLEYAMSGDEKNIDRERLFEIKSAFDVASAEIKSIIEYFDRAREYRLRELQSLLQKTEKAKRRKEERLRKKREEKEKTKDTNNPNNQYDSEKSSLSSTASSSAKPEENASSQTHTSSTIAHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6K9</accession>
    <name>A0A8J2X6K9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">V-type proton ATPase subunit C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_18690g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity. Reversibly leaves the enzyme after glucose depletion, causing the catalytic subcomplex V1 to detach from the V0 section.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the V-ATPase C subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6K9"/>
    <dbReference type="OrthoDB" id="6605928at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14785">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000002">
        <property type="entry name" value="V-type proton ATPase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1460.10">
        <property type="entry name" value="subunit c (vma5p) of the yeast v-atpase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1180">
        <property type="entry name" value="Vacuolar atp synthase subunit c, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004907">
        <property type="entry name" value="ATPase_V1-cplx_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036132">
        <property type="entry name" value="Vac_ATP_synth_c_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10137">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10137:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03223">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118203">
        <property type="entry name" value="Vacuolar ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00053565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF87971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="392" mass="43898" checksum="D0E438432F101D69" modified="2022-05-25" version="1">MSTTLYTANEFILLSLPKNAAPAVSPSSNADSWLQNEMLGGKSFVSDFRIPEFKIGSLDSLIVESEELAKNDNQIGSSIGKIVEILSGLNEAGTNTYKTVPINNVPVPEYLENFQWNSRKFRLDKSIEELMQLIANESSQLDADVRATNANYSAAKTNLAAAERKKTGDLSVRSLHDIVKPEDFILNSEYLTTVLVAVPKSLKSSFEKSYETLAKNVVPGSAGIIAEDSEYILYNVHLFKKNVQEFTSAAREKKFIPREFEYSEELIDKLKKEHDSSANLEQSLRVQLVRLAKAAYSDCFINWFHIKALRIYVESVLRYGLPPYFNTKIIAVPPKNLTKCKTELTEAFGYLSGNAFAKNKQGKIVKEDASLHQYAALVDTDYEPFVIYVVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6R4</accession>
    <name>A0A8J2X6R4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phosphatidyl-N-methylethanolamine N-methyltransferase</fullName>
            <ecNumber evidence="17">2.1.1.71</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Phospholipid methyltransferase</fullName>
            <shortName evidence="17">PLMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">BN860_16138g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes the second two steps of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylmonomethylethanolamine (PMME) to phosphatidyldimethylethanolamine (PDME) and of PDME to phosphatidylcholine (PC).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phosphocholine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32739"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64572"/>
            <dbReference type="EC" id="2.1.1.71"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32735"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64572"/>
            <dbReference type="ChEBI" id="CHEBI:64573"/>
            <dbReference type="EC" id="2.1.1.71"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 17">Phospholipid metabolism; phosphatidylcholine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Endoplasmic reticulum membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Mitochondrion membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the class VI-like SAM-binding methyltransferase superfamily. PEMT/PEM2 methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.71" evidence="17"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6R4"/>
    <dbReference type="OrthoDB" id="8300106at2759"/>
    <dbReference type="UniPathway" id="UPA00753"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000773">
        <property type="term" value="F:phosphatidyl-N-methylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000005">
        <property type="entry name" value="Phosphatidylethanolamine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03216">
        <property type="entry name" value="PLMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024960">
        <property type="entry name" value="PEMT/MFAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007318">
        <property type="entry name" value="Phopholipid_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15458">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15458:SF5">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04191">
        <property type="entry name" value="PEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005444">
        <property type="entry name" value="PEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51599">
        <property type="entry name" value="SAM_PEMT_PEM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 17" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 17" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 17 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 17" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11 17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13 17" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="14 17" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Lumenal" evidence="17">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="intramembrane region" description="Helical" evidence="17">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="17">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="17">
        <location>
            <begin position="80"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="17">
        <location>
            <begin position="128"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="17">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="111"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="193"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CDF88689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="213" mass="23935" checksum="10C4373BF7829E87" modified="2022-05-25" version="1">MDFKESFRGIRSAYMELFHDVDLEEPSFSTAMYFIIFNPTFWNIAARLEYHTHFLTKITGSAKKGCYLLAATIFTLGVGRDLCFHKALDAQPLSPVLDNPKVKFVGQALVIAGQVLVGTSMWQLGITGTYLGDYFGILMDHIVTSFPFNVCNNPMYQGSTLSFLGYSLVTGKAAGLILAFMVYAMYSFALRFEEPFTAKIYAERDDARSRKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6T6</accession>
    <name>A0A8J2X6T6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BN860_14400g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_14400g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="1664597at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="26S protease regulatory subunit 10B"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="26S protease regulatory subunit 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="210"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="63"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="433" mass="48875" checksum="19D39952A5CACA43" modified="2022-05-25" version="1">MSEENDPLMAAFEQENGQNEGNNNETHDLPREAEQEQEQQRLDPEEEARSKALNDFKKKLMEHRRFDDQLKQRRQNIRDLEKAFDKTENDIKALQSIGQLIGEVMKELSEEKYIVKASSGPRYIVGVRNSVDRSKLKKGVRVTLDITTLTIMRVLPRETDPLVYNMTSFDQAEISFDGIGGLTDQIRELREVIELPLKNPEIFQRVGIKSPTGVLLYGPPGTGKTLLAKAVAATIGANFIFSPASGIVDKYIGESARIIREMFAYAKEHEPCIIFMDEIDAIGGRRFSEGTSADREIQRTLMELLTQMDGFDSLGQTKIIMATNRPDTLDPALLRPGRLDRKIEIPLPNEAGRLEVFKIHTAHVKKAGEFDFEAAVKMSDGFNGADIRNCVTEAGFFAIRDDRDHIIPEDMMKAVRKVAESKKLEGTVDYQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X7D9</accession>
    <name>A0A8J2X7D9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BN860_12992g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_12992g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X7D9"/>
    <dbReference type="OrthoDB" id="16284at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004489">
        <property type="term" value="F:methylenetetrahydrofolate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.220:FF:000002">
        <property type="entry name" value="Methylenetetrahydrofolate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004621">
        <property type="entry name" value="Fadh2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053806">
        <property type="entry name" value="MTHFR_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00677">
        <property type="entry name" value="fadh2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754:SF3">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21895">
        <property type="entry name" value="MTHFR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MTHFR SAM-binding regulatory" evidence="9">
        <location>
            <begin position="313"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF88556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="599" mass="68671" checksum="A11879B76C624ABA" modified="2022-05-25" version="1">MRISEKLQTQHSKSEKPTFSFEYFVPKTTQGVQNLYDRMDRMYQSTLPQFIDVTWNAGGGVLSQLSTDLVETSQSVLGLETCMHLTCTNMPVSQIDEALESARLSGCQNILALRGDPPVNQESWKPVEGGFRYAKDLVKYIRTKYGDHFDIGVAGYPEGHPENVNDKETLLDHLREKVDAGANFIITQMFYDADIFVKWCEDVRAMGIQVPIIPGIMPITTYTAFLRRAQWCDIHIPQEFIDKLDPIKDDDQLVRDVGTDLVVSLCRRLLESGHIRHLHIYTMNLEKAPQMILDRLGLLPTHDEPLDNHLAILPWRKSLNPQRRNEEVRPIFWQRRPYSYVARTSQWAVDEYPNGRFGDSSSPAFGDLDLCGASLIRQSGKRSLELWSTPTSIQDIAQLVINYLRGELKCLPWSDTALNHEVDDILPDLVALNQRLIITVNSQPQLNGVKSSDPVYGWGPRDGYVYQKQYLEFLLPKDKLPKLHEALANDVMLTYFAVDYDGNLTTNHRDGSRANAVTWGIFPGREVLQPTIVEKVSFLAWKEEFYHICQEWKLNFENHQYNDSVNVLQRLINDYVLVNIVDNDFISSESRIFQLLSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X7K5</accession>
    <name>A0A8J2X7K5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ZYBA0S03-11694g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_11694g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X7K5"/>
    <dbReference type="OrthoDB" id="44015at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16988">
        <property type="entry name" value="ANTH_N_YAP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
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            <begin position="296"/>
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    <sequence length="643" mass="72773" checksum="629A8C2F34FEAE31" modified="2022-05-25" version="1">MTTYFKLVKGATKIKMAPPKQKYVEPILLGTTDPQDFNEIARALDERISDTAWTIVYKSLVLLHLMIREGEKNVALKFYCDHLSFFELNEISKCAKWSSADIRALERYNHYLKVRCQEYAQTGIDFVRDSHPSLKPGNGRDVGVALDNVDSLEIQIGALIRNRYSQMDLQNDMLLFAFKLLVQDLLALYNSLNEGIITLLESFFELSRPDAERTLDLYRTFVDLTEYVVKYLKAGKTVGLRIPVIKHITTKLIRSLEEHLADDGNMHPGTTGAGTTPVVGTGIGAGGSATGANGDLSIAQRKLEQIREQKRLLEQQLKNQQILVSPSVPQQPYNPFGSTNDSFSFEMASTGTSAQVPQATGNPFLQQQQPQHSQTQPPQIGFLHKQQTSLPTSTTVPDLSTQQTGFYAAHTQVTPSFTGAGFGGYSPQQQQTNPFSLGQDSSSPARANPFSQQQQQQQQQQLHQQQLQQQQLHQQQLQQQQLQQQQLQQQQQQLQQQQQLQQQQQLQQQQQQLQAQHTAQMQLQQQHTQHTGTFNPFQGQYGIQPQPQQQQQQPQVQHAVTTGSFVPQSPLPNLQNQHTSFHQQQGFVSTQATGYNPFQSSQVPAPQQQQVSQQWQQLPPQQQQQQQQQQQYEVYNQTNLIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X7N2</accession>
    <name>A0A8J2X7N2_ZYGB2</name>
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        <recommendedName>
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        <name evidence="10" type="ORF">BN860_12508g</name>
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            <taxon>Ascomycota</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
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            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
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        <text evidence="2">Belongs to the CDC37 family.</text>
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    </evidence>
    <sequence length="502" mass="58225" checksum="F8BF56012D380E08" modified="2022-05-25" version="1">MPIDYSKWDKIELSDDSDVEVHPNVDKRSFIKWKQQSIHENRAKRNQDIANMETQISMYGNLNKRVDKLLVSVSDNELADRQSVSRFLNANFDKTEKPQGPNVDPEIPPFNEMVEDLFEQLENDAKKQGKNPKDGSVIRKFVLEHRAKIDRVTAEAKVKLQELYKEKELHISSDDIKTGFDSGFLNKKETEEEERKAANMQKVAAANVSAQKGSEISLPKPRVQFIEYDDVMKLAPETEEFGKLKVGDYKASEKYLLAHMQIISEQQKDALMMTAFEYQMQDNPKRAYQVIHQSELLSYIREIYNTKKISFLHLEQMTEVIQLFFKRVLYNENNPAGRKSFLESVQTKFNHIKNRVKVMEQEQEQEGVETIQLKSLDETTELEVNLPNFESTDPEEQRKVAAFNKLPTKMQDALKTRNLEKVNDVFAEVSLEEAEHYLDLINEAEIIGVKALLENEEDFKHLQDEYKNRNQLEDLNLKEEHNQQSNAAEDKPSGLSTTDTVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X7S3</accession>
    <name>A0A8J2X7S3_ZYGB2</name>
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            <fullName evidence="2">ZYBA0S04-01398g1_1</fullName>
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        <dbReference type="NCBI Taxonomy" id="1333698"/>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
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            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
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        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </feature>
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        <location>
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        <location>
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            <dbReference type="EMBL" id="CDF89319.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1345" mass="139688" checksum="C03789AD2B3ACF38" modified="2022-05-25" version="1">MLFRKFKKHDSENAGDESNEAVRRSENDPERTVQGGQQEGQQEGRNLSSKGHLGGTGSAGLHSAEAGAAGTGPTPPTPPHGQDSGEQYVVEQSYKEEYVPSSRYGTAASPRQGAPGVQSTSQNTSRGATSYSTSKNNSKDVGSSGATSGASNVASDHPDYEDRSDRHSHSKKGFSLPFMKGRHHKQEEHDHAQEHEAMAGERGQAHPSSDRHDNHNSRAAGAAAMGTTSRSRGATSGSSNSAPLGGSSPSGLSAGVPADPGTSSATMAGAGAAAQRVGGPGTGVGTGSGFNSNSNSNSGYGSTTRTGGGSGSGLHQDSSTGIHGKGRSENQPRARASSDHGGRNSSTKQSKEVSGRDNSGGITGVSKRVSGAPGATTTTTTVITSDTTTSNINQGSSADSSNQGGKNTTTSTSTSRKGAGDYGFVSATSPSGTRDFDYDKIEGDFPEGTQKYEHRGPATASSAAKKAPLSAEEIEPRAAAHGHGSHAYSESYRGGYQAGYTAARSGGVPVGGTTQHSPKESHGGHGEEEAAGFGKTAGHSNANALGASGTTGPSTGSTGPSTGTTRTTRITTTSTAPSSSRAGPAVTTSSAAKSSSSSSSSSSKYGGSGAERYGAHDPSYQESRGEHTGVLGGTEEYGYGKANKPSDQFGGDDFTREEKPGLRHEEHHHHHVPGAYVTDDRDVDDHAHYDPAGYRKSAASQLGPGSSEYPEDYDDMRNPGSDPLGTRRSEGSARKSGAPPPLAVIDPANPKLSENIDASARKSGPKVTSHHAIGSGARTSGYGDEYAAKAGGAYGDSSQDVRPPKYSTGDRGVEHDSDHSHDRRHDDSTRNSSSGNTGGGNYYRHTEYYHSGPGEDQSALRPGTYDSQDDRGQWSQEGKWGQQESLGQATRNQEPRTASSGYHAKSSGAREGSPSGSNYYDSSEVAPDSEKQTAAERATSKKSVRKEQEPVLKRPLSKNQDTSVPRLGFEGLFGRRKSSSSGGGSEEPGKMDSFVRRLSLSRKKSSDRSEPSETTTTEYYQEEEVEEEIGQQPLSKTKSSKSSKSRKDSSSAESGSPKLKKSVAETMSPKTASRKSVSGTTSPRAKKVQVPETRSQPQMQTQTQTQSHSGPTPTREPPTQEGKRFAETFGNLPSLIDPNVPTYGFGTHSGTQDASTAKPVASVEPAETSKSKFASTGMSSPSAGGGPLSGSIGRDTGKTSTKSKGSMGPGTTSNFSAMPPTSYSTSAKRDEPTYADTGAEGPGGNYVASSGNNSLGRTLSVESDNSQYGRTHQEGYHHHAGESPLYGEHHHQGYNESTVPRAGVVDDEELPEGAGHAHTGKEQPGIFEKIKHTIMPGSDDTTTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X7T5</accession>
    <name>A0A8J2X7T5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_01244g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_01244g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4070435at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1157"/>
            <end position="1191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1392" mass="157843" checksum="F4A64474AB9934E2" modified="2022-05-25" version="1">MAFGNAWDLDCLRPESWFRAGDLSFSESATRPFISSPRHDTQAELNDNDVTSLENSDINHFRLWFKLRSSICSRKPQRCLPIMVANNPQEHVQQISHIRTNISEAESPFFGPRREMEELFGVTGDPESLDDSYIVELLASSQPGDTNNTLKDKMNKISLLPTYLLVSLNSVYIAGEPSHIPINHGIIDCQIFDTLPERLGSLILISTSSGYLISIILGIGQSKAAIVQYWELGSAGDWHIVKHQSHEQFVAVNKKKGLCKFFCFRNALHFTLVNNLSIDNTQFLDCLFFPNTSQSHYLLFVPSIRYHRLVFFCIEWDNTAPAMKDVYQLTYLNGHLIDCCVPVGNNRSLVFHGSDISLISAHQIMSGETNFKHFESKLLKGVCSYFEAPQLLAKLKRIHGEPFNTFQYCTLLATSSGNIIICVMDDQDRIEFFSLTRFKGLKDVCPVCAQSASEKEYELVVISFGRTLLLTIDIADITRLTGEFPIPSLSGIQFKHTLDSSTEDNFKLLVISPPKTASRFSSEIWLASSMAISHLQTFAPVRKLHEVCKLRQFQLYNKIQLFRFSSLENGLKKALLQDGHIQHPLMYLVVATDSLSVSKAFLLNLHTPEITVVELDDFLAEVEGGSLDAFFSQKGSLVQITRDSVYLDLLGLSPEEQTEEYSPGWEIEGVAHCGNKIIVWNCVKKKCNVINNIDDLGKGQQFVTSVFLENEMRLHNSKSVEFCIVRDEENANTTFVYLATNSGLSRCTWDALYTVNDPTFEFQSLCDECSDSLLSLSSHLCFIKNDSQFVKANYRHFHIAEVQLRYNTKDFQIRPFDEESCLIFSTQEITVYSVSATKEKGTEIYELKLPPSSKVNPILDVSADAENNRIFVLYSDGLEVYDLSYFTWNSSNYLLRSTKTTEKKFLFVEKINRMIVVNLRAREWDCIKLVDGKTLSLDPSTLQSPADLKLIDVVEIYSAKRHVELLLQFGTLLKLVRLVPKRGRISVQEMSRYEFSAPLFNHIEVGNDGRFLVFLPAIPASPHHPFDYFMQMHVDQNDQLELLSSLKFPEQINLQCFRLCGKDVIIVSKVYDRVFLLKDFSRQISQKYLQAISLKMPLSSKVLLVCPLGGDSFVVVVSCEGRSDHITELLFYHRDDVKLSGESKKAYADGGDLLYEAAMLDDAQRQVESEISRIEEEEDDAQENFNEHEEMMSISAATQGDDWDLNYRDFLHRMANGRLGQGEEWDFDDLDMEYEVEEDDEVAQGSEEDEDMLDTDDMDEMDEFDAFERGASNSFPLDLDPELEDSATAPEVASADLVRPVTASTNRKPYQVMHLTKAIKDIKYDCNANAIYLLAMDSSVLVLSQASKQIALQSIVQKDTSKTRDNSFPIKTTQSGLFALDADGHVPELPWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X892</accession>
    <name>A0A8J2X892_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Deubiquitinating enzyme 12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Ubiquitin thioesterase 12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ubiquitin-specific-processing protease 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_10638g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 17 18">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X892"/>
    <dbReference type="OrthoDB" id="292964at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000180">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000203">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2230.10">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00695">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DUSP" evidence="14">
        <location>
            <begin position="30"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="13">
        <location>
            <begin position="289"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="749"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1052"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1138"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="749"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="765"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1065"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1143"/>
            <end position="1164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00075183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00078780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00081974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF89703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1180" mass="135158" checksum="E1ABDDDBD25B9D0C" modified="2022-05-25" version="1">MSDSSGGELSIENEPVGVTDKEDLVNDVFPSLDMQRNIITQLLNTNEKQAKEGDRVYIIPQLWYAKFLDPQIVESKQLGPIDTSTICRDYDNFILVDYERCPYLSLPESVFVKLIEWYGLAINSQPVCTSLILDENNNLVTEYNQCVFRVHHLVASTYERRYNNRKVAYFAISKLSTLKQLSIKILDTFFDRETELDISKAKFKIWFVKDSDANSATSVLNSSYLLDPLQFMGFPVHTRITPQLFARTLKDTGIFTGDFVVEIRQSNQNYHWISNYFMYNELAPSSGTTGLSNLGNTCYMNSALQCLVHIPELRDYFLYGGYEREINTENPLGYQGYIARAFSLLIQNLFGNRASISANHTASTACSPTNFKNTIGHFNSMFAGYMQQDSQEFITFLLDGLHEDLNRIVKKPYVEKPSLSPEDNKNDFNVIKKLAEDTWKLHLLRNDSIITDLFVGLYESTLQCPECNNISITFDPYNDLTLPLPVNTVWHCKVKVFPQNSPPCILEVEMSKSSTYQDLKEYVSKYAEIDSQNLYGCEIFSHQFYNNYESPESNSQFLPIQELISESDDVIFYEIQASENDIIVPVLNSRIEEGFKSPRLFGVPFFIVLSPQDRTNPGAVRYAIENYYSHLSGGFVEFPLQRPDKLSSSSTLAFLSERYPNVDFDEFKDILQYVTRKDIESVDCYFKIKIMDNSPSEGETDRELLSDAEKSEPEFWTPFSLTNLNKSKDISDFLDPVIRNIYEHPATFYRTQSNSSQETSLEDSSTTKEKREDMELTQHDSDIDLDNQKQENDLGLHQEFGQLATSSPRTLIVHSHSVIVCEWSDEAIQEVFSEDKLIHWEKPAELKNSQLEEERQKRQQQEERRITLHDCLKLFSKREVLGLNDSWYCPTCKAHRQATKQIQLWNTPDILLIHLKRFENLRSFSDKIDEVVDFPINGLDMSQYLVNKGDPQGNIYDLIAVDNHYGGLGGGHYTAYVKNVSDGKWYYFDDSRVTETSPEQSVASSAYLLFYIRRTKDGKIGNNQLQEIITKGREQHILSVQEMHARQRLIYDTNKTDSEEEVEHEDAGRLGEDNFEDKRDNSEPNAESGGSHENSISARSTDYSVKSLEVGRLQPSEPYDGSENNMSRRKLRLLNKTYTDTNIAHSPASSISSDGGDSIGASVSRSKSKDDTLLRSPSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8J2</accession>
    <name>A0A8J2X8J2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ZYBA0S04-06722g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_06722g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8J2"/>
    <dbReference type="OrthoDB" id="3976101at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF89539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="552" mass="63949" checksum="0238AF173D4A9AD7" modified="2022-05-25" version="1">MVTQDQLLTLLSHSSLFFNQYDLFNLALTSKKVHDEFATKKLYRCIHITRDPIIRSPFWYLDGNTTYLSGYRAVKKTADQNDLFLHDRIERLLESPHLDKIEEIIVEKGLFADYETCTPILRKLLDQIVVLNKVKTFEIRDQKLFRDYYLKSLELTNLNRVTIVDLNSIGTLRSPLHLRKLKWILVKRDMTGTSLAPSLKQILCEQLEEAEFVADEVTCCSLNIFLYLQKEGVKFQQLRSLKFNYTHNLNGCDNSFLEDPISVIESVVDLGKLKKLELGVSCEHADCDCMDAFLGGLAPKLNSLRHLGMIEKNTMETANHKTKENWDIITNRFVMRLPDVSQNLLTLSIRHATPLNGLSEDSVAGNYVRRRTLFETVLPKLTSLQNLIAPNILQSLSAYEFLVCDLLWNGCECNACSKILPIFDQYLMNHQHYLIDEGRYTDVTPTVFFAYAGDFLMQRFSNYLDWDLDIFSIAPSFRFWNLHGYDNLHHFDDYECFFDETTFAPLCMAISHFFNGYMDHLVHLLPNLRTAVLSGIYYAVDSDSHEYISVYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8U0</accession>
    <name>A0A8J2X8U0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ZYBA0S05-02036g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_02036g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF89802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8U0"/>
    <dbReference type="OrthoDB" id="2399539at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052202">
        <property type="entry name" value="Yeast_MetPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782:SF12">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="40"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="79"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="757"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="828"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="972"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1087"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="93"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1088"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1126"/>
            <end position="1138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF89802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1196" mass="132789" checksum="A7626D3480F26F8A" modified="2022-05-25" version="1">MGRPKKEVSQGNLERFNKELEMAGTHTELLLRDKKGRSRSCLLCKRRKQRCDHKQPSCTTCLKAAVKCVQPVRYNMPTGDTEGTPSPLPGAYSGSNTSNVAQTPKAQKNGIISPDSGATSNVTSRATSRAASGVASGSVVNVNSEAASGASIGAMNGGASMPSSLSSGHSTAASPELRSPSLNSSTFNLYPADLGVLNKPLCGQGAKLNPAYTLSLERRVKYLESLLDTSVETLSNPTNSDKNANILPLPNTGIKDKFLPLKKPATNHGDSPLSIAANLAGPNQSTILPSLASDSLDSVEFLKCIFAKYNLREFLDYDPAFRFEEEVSRSLLETFFCRLQFKYPLLDEDEIYTFHDDYIHSNIYSYSENGFHFACGRLWLVFSISACLSMTTGKYKGEPPVRYFSRAIRHITRCGENLTHVQQVELLTLLVLYILRTDRDSLVLYDIIRDAMIICRDKLHLNVWRPEDPMAHKNLRLFWCVYLLERMICVAVGRPYAIAESEIDLPLFDEVSFNTRTMNDPKRQHKGVHFINQSLRLRRIESHFVEKLQILPESQNKKQALSEQLPLVQKFFHELEVWRANCSKSNVRNFENETLKLYYYRSVRLLIQPYLEFLEPEDRLFRECQAAAGQICQLFKIFHQKTMTGHSTPAAHTVFIAGVTLIYCMWLARNNNDQKRKNLGDESKHTRPLVSASLFSTMDDLRACSVCLYVMTERSSFARIFRDTFDQLMNATVGNLIERCGPNSSELIYISSLAEGTTDTNKGEGSNARGIEQSEGGMPPATRRIYGTRQAEVLAGFVENSQVDLEKQKELKKKQGVLEQKSVPESLSHFLINRSDKSSSQESSSQEITESESTEKTATAKKRRGSRCDNDSLASDENQYIVKKPTHSKEYDWKVFQHQALLQQHNAQQNLQAYLSSLNGNNKAEQSLDGIKTCINDIEKRFAHTIGPSNGYILSEIKNAGNNTNPVDFGRLTGPVGPPGAPGAPGSSGLSGPPGPQNSALPPMLNPEQNYRVLGANSTREGFAISTSKPVAKLLARKEPENSATILNANILLSNGAQDMIKDISTWTTDSVAHLMNDNNYKRDASYEDSNNAVQTQSSEAHRFPENFHSSTKEISSSGSKKRSMEPTTSYPQSTSLGFLPVSNNGSIPVTSAADADQIKLSVDPEFQSVKLPPPTAKDFVVEEFWTVNDDYGFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8U8</accession>
    <name>A0A8J2X8U8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ZYBA0S06-05490g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_05490g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8U8"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF52">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="13"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="102"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="147"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="102"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="229"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="244"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF90305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="508" mass="58610" checksum="2B43FFB56D5A3C66" modified="2022-05-25" version="1">MASQLKIRLDETTYYSTLGLSSNASEKEIHKSYVKLARELHPDKSKSDVAAELFKLISHAHSVLMDKEKRVKYDQLLLSKGLLHHSIKETYHRFDLRVEPVTPAKERSARPQRPYEKQPYGFNTDTKSPPKGKVPIFRSFNLKSYQRESFEHKEQASSSQRRDDGIFTSRPSTPFNAGQDEKAEGKREEDEEEEEEEELNNSRSKLHKVNLSSTFEDFSNSPFIDRDRRHYARTKHEAKKQGRRSTSPIKTVPTTSKTGTHEGWESLKSLLEKFRRQDAKEELPTTEAPSGKANESDLSAEKIRRAESQSIKLDDLGQSLPTEKNTFDMSHVSESIPAVKRAKLDLKISEEVPKVEPYKDTQNVAESLYVPVNRPLPRIYKTHHIPLDQYMINSRSLQCELPEMPNFQCNVMNKAEIEYCKELVMIFNEQSNAIKQNLLATLSDRLKADQELHEKLVKVENTANWVSSKDFDFELVAKLSELQNRQRVVAQSFADLLKTVYTITEQHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9K2</accession>
    <name>A0A8J2X9K2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Serine/threonine-protein kinase TOR</fullName>
            <ecNumber evidence="17">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">BN860_15874g</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Vacuole membrane</location>
            <topology evidence="14">Peripheral membrane protein</topology>
            <orientation evidence="14">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="17"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010972">
        <property type="term" value="P:negative regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016242">
        <property type="term" value="P:negative regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901992">
        <property type="term" value="P:positive regulation of mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031137">
        <property type="term" value="P:regulation of conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905356">
        <property type="term" value="P:regulation of snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05169">
        <property type="entry name" value="PIKKc_TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000020">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.150:FF:000001">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000371">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000601">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000004">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.150">
        <property type="entry name" value="FKBP12-rapamycin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009076">
        <property type="entry name" value="FRB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036738">
        <property type="entry name" value="FRB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057564">
        <property type="entry name" value="HEAT_ATR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024585">
        <property type="entry name" value="mTOR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026683">
        <property type="entry name" value="TOR_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF9">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08771">
        <property type="entry name" value="FRB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23593">
        <property type="entry name" value="HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11865">
        <property type="entry name" value="mTOR_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01346">
        <property type="entry name" value="DUF3385"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01345">
        <property type="entry name" value="Rapamycin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47212">
        <property type="entry name" value="FKBP12-rapamycin-binding domain of FKBP-rapamycin-associated protein (FRAP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0469">Meiosis</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="FAT" evidence="20">
        <location>
            <begin position="1327"/>
            <end position="1911"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="19">
        <location>
            <begin position="2085"/>
            <end position="2411"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="21">
        <location>
            <begin position="2430"/>
            <end position="2462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU364109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CDF88678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2462" mass="279693" checksum="D7F400981BD8037D" modified="2022-05-25" version="1">MLSLRRKAGGHHHTRKKLTSSSRSADDISTTSAHDGTGIVTADETRVTNSSGGDVKNYVDNDASLAAMDSNFSTFNLIFDKLRSPIPQERIAASYELKTSLISLVREVSVEQFQRFSNKINNKIFELIHGSDPSEKIGGILAVDTLIGFYAHTEELPNQTSRLANYLRVLIPSNDIEVMRLAANTLGKLAVPGGTLTSDFVEFEVKTCIEWLTTSPENSSSSSKQEYRKHASLLIITALANNSPYLLYPYVNSVLDNIWRALRDTKLVIRMDAAVTLGKCLSIIQDRDTALTKQWVQRLFKGCVYGLTLNTNESIHATLLVYRELLTLRGFYLDGKYDEIYQSTMTYKDHKYDVIRKEVYAILPLLASFDPQLFASRYLDQTMIHYLTILNNMSTNATNHSDKAAILVSIGDIAYEVGSSIMPYMDPILDNIRDGLQTKFKNRKKFEKELFYCIGKLACAVGPALAKHLNKGLLDLMLLCPLSDYMQETLLIINEKIPALESTINSRLLDLLCTSLSGDKFKQPGSPMSLKSLSIEKARQWRNQSILRKTGEVNDDVNDAQILTQALRMLHAINHKYCLTEFVRLVVILYIEHENPHVRKLAALTSCDLFVKDNICKQTSLHALNSVSEVLSKLLTVAITDPVAEIRFEILQHLDSSFDPQLAQPDNMRLLFMALNDEVFAIQMEAIRVVGRLTLVNPAYVVPSLRKTLLELLTELKYSNMPRKKEENATLLCVLISSSREVTKPYIEPILDVLLPKSQDNSSAVASTALKAIGELSVVGGEDMKQYLDELMPLIINTFQDQSNSFKRDAALKTLGQLSASSGYVIEPLLDYPELLGVLINILKSESAQNIRRETVRLIGILGALDPYKHREVEVTSNAKISVEQNAPSIDIALLMQGMSPSNEEYYPTVVINTLMKILRDSSLSSHHTAVIQAVMHIFQTLGLRCVSFLGQIIPGIISVMHSCPPSLLEFYFQQLGLLVSIVKQHIRSHVDEMYEVIEEFFPITKLQITIISVIEAISRSLEGEFKRFVPKTLTFFLNVLENDKSTKKSVSIRILKSLVVFDSNLEDYAHLITPTVVRMAEYSPGTLRMMAIVTLGKLAKNINLSQMSSRIVQALVRVLNTEDRELIRATMNTLSLLLLQLDTDFTVFIPIINRTLVRNHIQHSIYDQLVNKLLNNEGLPTNILFDRDFDNNKKEPAEAESAAKKLPVNQSVLKNTWDCSQQRTREDWQEWIRRLSIQLLKESPSHALRACSGLAGIYYPLARELFNASFSSCWTELYTQYQEDLIQSLCTALSSPQNPPEIHQTLLNLVEFMEHDDKPLPIPIQTLGKYAQRCHAYAKALHYKEVEFILEPSTSTIESLISINNQLHQTDAAIGILKHAQRHHDLQLKETWYEKLQRWEDALSAYNQRENAGEDSLEVTMGKMRSLHALGEWEQLAELSSEKWAISKPDVRNVMAPLAAGAAWGLGQWDKIEQYISVMKPQSPNKEFFDAVLALHKNNFDEAEKHIFNARDLLVTEISALINESYNRAYSVVVRSQIIAELEEIIKYKKLPLNSEKRLMIRNTWNKRLLGCQKNVDVWQRVLRVRSLVVKPKQDMQVWIKFANLCRKSGRMGLAQKALNSLLEEGGDPEHPNTARAPPPVVYAQLKYLWATGSQKEALRHLIGFTSRMAHDLGLDPSNMIAQSGPQNSTIAPHHVEDYTKLLARCFLKQGEWRVLIQPNWRIENPDAILGSYLLATHFDDKWYKAWHNWALANFEVISMLTSGSKDEKANGMRSSTNGLHRIENGMMGSDTFTAEGSNYPIELIQRHVVPAIKGFFHSISLSEASSLQDTLRLLTLWFTFGGIPEAAHAMHEGFSLIKIGNWLEVLPQLIARIHQPNKVVSRSLLSLLSDFGKAHPQALVYPLTVAIKSESVSRQKAALSIIEKMRMHSPVLVDQAELVSHELIRVAVLWHELWYEGLEDASRQFFGEHNTEKMFATLEPLHEMLKRGPETLREISFQNSFGRDLNDAYEWVLNYKRKKDVSNLNQAWDIYYNVFRRISRQLPQLQSLELQHVSPKLLAAHDLELAVPSTYAAGKPIIRISRFEPVFSVISSKQRPRKFSIKGSDGKDYQYVLKGHEDIRQDSLVMQLFGLVNTLLQNDSECFQRHLDIQQYPAIPLSPKSGLLGWVPNSDTFHVLIREHREAKKIPLNIEHWVMLQMAPDYDSLTLLQKVEVFTYALDNTRGQDLYKVLWLKSRSSESWLERRTTYTRSLAVMSMVGYILGLGDRHPSNLMLDRNTGKVIHIDFGDCFEAAILREKFPEKVPFRLTRMLTYAMEVSGIEGSFRITCENVMRVLRANNESLMAILEAFAFDPLIHWGFDLPTQTIMEQTVIQLPLTNPSELLRKGSITVEEAAKMEVEQKNEIRNARAMLVLRRITDKLTGNDFRRFHELNVPEQVDKLIQQATSVENLCQHYIGWCPFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9W4</accession>
    <name>A0A8J2X9W4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Glucan endo-1,3-beta-D-glucosidase 1</fullName>
            <ecNumber evidence="4">3.2.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Daughter specific expression protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">BN860_01618g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 81 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="4"/>
    <dbReference type="EMBL" id="HG316462">
        <property type="protein sequence ID" value="CDF90959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X9W4"/>
    <dbReference type="OrthoDB" id="4473401at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:endo-1,3(4)-beta-glucanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000920">
        <property type="term" value="P:septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.420:FF:000008">
        <property type="entry name" value="Endo-1,3-beta-glucanase Engl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000006">
        <property type="entry name" value="Endo-1,3-beta-glucanase Engl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005200">
        <property type="entry name" value="Endo-beta-glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040720">
        <property type="entry name" value="GH81_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040451">
        <property type="entry name" value="GH81_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983">
        <property type="entry name" value="ENDO-1,3(4)-BETA-GLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983:SF20">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17652">
        <property type="entry name" value="Glyco_hydro81C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03639">
        <property type="entry name" value="Glyco_hydro_81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52008">
        <property type="entry name" value="GH81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0134">Cell wall</keyword>
    <keyword evidence="12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035266721" description="Glucan endo-1,3-beta-D-glucosidase 1" evidence="17">
        <location>
            <begin position="23"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 N-terminal" evidence="18">
        <location>
            <begin position="578"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 C-terminal" evidence="19">
        <location>
            <begin position="911"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="87"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="262"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="104"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="329"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00074614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00075210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF03639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF17652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CDF90959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1277" mass="136019" checksum="836D718AB48D6D6B" modified="2022-05-25" version="1">MIIGTPAILLFQLLSLTTCTRSLEVALETDIVLSSSNTIFTAGDPYIIADHVVPRVSATDQGSLSLLSVASSSNELNTKSASSSLSFTSETVRSGGSSSATMITSRSVSTTSTSETTVTVLPSEGDRTTLQPLSVVSGVLTVPYDSDATTSTSTIFTTSMPLSVSQTLGSILTSLTTNSNPLVTNSPSYSSQKITINTNTLTSALTISNSVVSIGPSINSTNNSKLISAHTAGKSLSLAPSGTVNSDSYIVSTEISRSANWTQGPYDSDTLLHPTVTQNSKQTGTSSASSYDLSDNHDTSIGSSISRWSTPAVNLTSHVTITPNYNQTTVSSSTIKEGSKTASMKNGTSLTGTSNSVQFSSSSYSSLLPYATPNPNFNQTATLSSITSQNSAATGSSNSTKSIGSKNSFRSISSSHTSQLQTLASTSNSNQFSALPSTISYANESTASSTISYGTARASNRNTTTLVGTSGSILSTSESTLRSQLTSASLLPSSASSTKQSLYSPVISSSSLFSSETFSKSGSSSTVSSWTSTTSTSLQQTITSKTATSETSTYSTISLFDAIATDEPPSVFGRSMNPLSPADGVSNDGPIETNKFYTNLLVGTQSSAVFVYPYSLWKYSSSYGTGFAIQHTTSSQYSFGDEDSEGNSEYLVNPLGIAHCVLSATSFDSSMTMEVNEMTFSSVKVTLTEGSSTSDYLEIPLVQGMGFSTGVYHGGLIAKIASSVGFSAVTEESSTNLPEGVLKYRITLLNGVTWLCYVVNPSGTMESSFSMEVTSTYTIEAADSIDGLIIQLAVAPESSDLEEFYDEAAGMYPTDFQVSGISDGTTATYEFSYNTEGSSASGNTIIFSLPHHQKSFSSSMADKYTGITLQSTTKGVMTGYLTNVLQFSQTMNTEISWLPWSSQLGSDPLSYSADQLKLLAEVANSELQVSISSSISGLNTYYLGKVIDKYAYILLTVSDIIGDESSTNSTLENLKSAFDILLSNSQTYPLLYDTRYGGIVSSGDWASTTTQYDFGNTYYNDHHFHYGYIIHAAAAIGHVDAKLGGTWAQENKEWVNSLVRDVANPSTEDQYFAQSRMFDWFHGHSWAAGLFENGNGKNEESSSEDYNFAYGMKLWGSVIGDSSMELRGDLMISIMSDSMNDYMLYDDSNTVEPSEIIGNKVSGILFDDIIDYTTYFGTNTEYIHGIHMLPITPASSAIRQPTFVSQEWSSKLSSIIESLESGWAGILRLNQALFDPLDSYNFFSASDFDSSTYLDNGMSRTWALAFSGGLANSLGLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9X8</accession>
    <name>A0A8J2X9X8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ZYBA0S03-05974g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_05974g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X9X8"/>
    <dbReference type="OrthoDB" id="341482at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037700">
        <property type="entry name" value="NUP88/NUP82"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13257:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13257">
        <property type="entry name" value="NUCLEOPORIN NUP84-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF88962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="692" mass="79398" checksum="B04A9E318FC976A3" modified="2022-05-25" version="1">MTIESHPIFRRSVKSPERFFFTSRNGSRVVTYQDSAVRWCYASDPSYNFMPLEPPLLHVDNAKLSDTGNLLLLYNTSEIRVIEIPWGYHSIPHQSFQKFSLTAAEQDPRIKQVIFHPLADQEHSIVVLRSDSTIALLNINYPETMQPHEHILNKYNGALGVDSLICDIESITFSLDGLILYALSVSEGGDIYAFYPCVPHLVSLQADELDLLLSKSLLQYDSLDVQTPSATKRNTVKQFKFVTQLRKKLEEQETTLTITPEWLRVKGQGPFTIAPFPDRYYNRTSKQIALLPIGPYNELLLMSLDDGTIAVLYRDLELIMCWDTNGYTYNNSFVLVESIKLDTRNILVLPHTHGQFYVLGEGKAYFIDTTCWSQTFSSCIDNSDLQPIADLTFKSKVDKIDIPGDIKSCAIWDAADQPLHVFVSDKTVYAKTMTQDTFDESKQKEDLQKPERAVYKPLFSQPLAEIMAYNTTLQAEARHPVAKLIEPPKRQKPFLNDSNEEQLDILTDVSKEFSQKVSIAQSLGIMLHGRFIEQQTELTRQLRFSNSLLERQENLKKKSEMQSSECDAKSAKQAKLLARFAKLKENLIRIGESPKFKDMTINNKEMAWFREIRNQVLTFNQYVHQQKNIQDQLRYIKQELDHISEESDRGPKEPSDEWKELLTILENDTKIIKECNSELAQTFSEIGTEVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XAM8</accession>
    <name>A0A8J2XAM8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein STU1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_01640g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLASP family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316465">
        <property type="protein sequence ID" value="CDF91557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="46159at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060172">
        <property type="term" value="P:astral microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF9">
        <property type="entry name" value="CLIP-ASSOCIATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CLASP N-terminal" evidence="8">
        <location>
            <begin position="300"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="594"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1078"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1110"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="594"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="633"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1087"/>
            <end position="1097"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF91557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1462" mass="166483" checksum="1499F49A8792222D" modified="2022-05-25" version="1">MPDTFAVGFPLYEQLSQSGDNDPVLLLTQFKGHVKKELVDVPSIPNYFKGLLYVLDKPEFSNLPRISTLAHSSLSYLVKRVAMQSPAYFEDLNAIRNLVAHLFMLGKESNIQLEARSFWVSSSRALEAIYLIQPVALQNCLEELLKDNDAQLRKLLLVMQELLQISQKVEGDHSREAIFQRFVPLLVHILNQKEYEESEVASLVCDILIKNNTEKNQLQDIAERIGQPHNKVLFQKAAAVVISDGTRPSSFPDPPLLNTQSELQCIYDEFQVFTNHYSCSNVVQARELSEESMEYVQSLLETLLVPFQSLKETEQNWKMRQANLIEMRGLVQNKFVMEHQLEFLSCCRELHLMECVGRAALSLRTTLSTTACLAMKDFIQKFSLNLDSVLLEQMFESLRALLFSSKKISSTNAFNCLVIMLTSIDFHNKLFQNCFMLINQKSTLPKNCSAILLRIFIIKFHNTSKLESSIIYIEEWLKKGITDAQTSVREAMRKTFWYYYKCYPNSAKKFLNSQLSLQLKKAVELSIPHHLHIDYQPIVSNSTISSLSSSLSSSRRTSFGTKNFPSYAKPTQSSNAFLQRVANARSTSEYVLRENNVNTHVNNSSKRKISAPPQFAKRSLTTPTHSDIHHHTSGPTDQSPAKILLSSQGDDSNSTFLEQHNEPTENSVQIDLTDDISRNHSNSLIKKYVGYKVGDGKGNLETMYQYLESSSLIKIKDGLQLLQNTLLMDSSQVADSRGTLDFERLTPLVRHLTIKVPQELKPLLSIPKFCQSIPLTYLIEIFAINHLDFADELLETLSLETSLRTVCEIIAYLDSGDKNQENESSPTVSLHYMKYKQFIFNFCFKLVQKFLNNKSHDVAECHEALGECIEKLAQICGQEYDETLYYDTIHQVYVSNTPLFVEKIRHVPLVSTKLKICNELEARDKEGNFHREVIVSRQSLDGLDGDRFTDAYKEEVVDEGKFMEMTMVNPFNQMRAASGGSVVHHDHTLSKTVAEADNPSNKSDEEYDDINQKGLSEITKVVSIYQPAENVSKPDVSRKMIEEQDVEMSGVLQETSVNLSEIFGTSQEHETTVKFSKHPPNIINPATRPSSCELTDSASRDEDHDLARNVSIERDKSPVTPLTEHQSKELSNGINSIDIGKKHEVTVSREKKSICNLSAEILANAIKENDNNGDPNTIEFNLLELVPSDSLIYHEISQAVITTNDFQDFNQLFLHMRKAITRIESKSFTMKHLNYVIGSLILGIRQENMRHWLELENGYNELLDLSEKLFHSTDETQMVPVNLACKTIVLIESLVLTNNYLQNVTPISSSVFKKIWEDVILMLSKLPEYSSEIYCLLQELRDLLVFLDFFTKADVNRILRGLASEVNDATLGIKETFLMETLSIILSKSRIPLQKRLIFDIISVIQSYLEAKKTEWRYFSCKVLSQVLQHSEPFDNDNTGINQLFNGLSSGRQNILRRFSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XAR7</accession>
    <name>A0A8J2XAR7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Lysophospholipase NTE1</fullName>
            <ecNumber evidence="3 14">3.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Intracellular phospholipase B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN860_03202g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the NTE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="3 14"/>
    <dbReference type="EMBL" id="HG316464">
        <property type="protein sequence ID" value="CDF91453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="421051at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046470">
        <property type="term" value="P:phosphatidylcholine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000007">
        <property type="entry name" value="Lysophospholipase NTE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001423">
        <property type="entry name" value="LysoPLipase_patatin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056556">
        <property type="entry name" value="NTE1_P-loop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF29">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE SWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24179">
        <property type="entry name" value="NTE_Ploop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01237">
        <property type="entry name" value="UPF0028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 13 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13 14" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="11 13 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="16">
        <location>
            <begin position="782"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="16">
        <location>
            <begin position="899"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="17">
        <location>
            <begin position="1332"/>
            <end position="1496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="360"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="500"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="580"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="695"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1618"/>
            <end position="1637"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="13">
        <location>
            <begin position="1336"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="13">
        <location>
            <begin position="1363"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="13">
        <location>
            <begin position="1483"/>
            <end position="1485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="362"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="500"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="527"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="541"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="583"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="696"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="722"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="13">
        <location>
            <position position="1365"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="1483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CDF91453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1637" mass="182130" checksum="3542AEE875539963" modified="2022-05-25" version="1">MNVSNQTKLIPLRGSDNAPGFVSSSQSSTQRQMSQSGQQAASSNSQGWLVDNILNVTWVISYFLIGSTRYFARWSIYMVTVCLLKVPKWCFLSANQFHLTISFPAFLALATIFTYMVYCLMKDQVLSQYKTLSSDDVDSNEADTTHSSTNGGISDQKDEKLGIRGQLRYGKREKDEESFATSYLDQFLSAIRIFGYLEKPVFHDLTKNMKTQKLDEGEILLLDNSVGFSIVVEGTLQIYHKLEQDSDIVRPNEAYKRKMDNITDKTEYGDRESLSSCANEFTAGSGNDFIDNEEENSSNYIELKNGLGKFQLLNTVKSGNPVSSLVNILNLFTSSNVGDGWGLAAPSSLSLNDKPIDFRSPFATQNDWNQGSRGSNASNSPPPDLEKSVDGEPDLMQTYSETNDMPQGLMPKVIACASTDCTIAIIPPQAFAKLTAKYPRSASHIIQMILTRLYRVTFQTAHDYLGLTRELIDMEVLLNNTVSYEIPFYLKETVMKKLKSKSEKEDINESRMEKKKEQKVQNSVKKGPARPKMAHLKHSQSHFNSSSTNLRSSRHVVLDSRDHFNPGDLLSSVPLLRKESLNPSTASSTQESKSSSPPASIPSSQRGVSSTSSKSSNKLLQKPPHSSAILADSSHNRNEPNSSHANLLVQREENIKDRSFSAAEEETEESAVKMALVEAMFTYLGINKENMRFPSVVSSSGTSDELTGTSHRSSSVSPDATSSMSLSSYASPSLTNGASYTSVLRILPSDYAISSRRTKPRKKAEYKEEISPKLTFESAKNDFAQGIKLQFFKQGTTIVEQNRKGKGLFYIISGKIAVTTGKGSRMASDEKSLNQEHPETLLYIVGAGGIAGYLSSLIGYKSFVNLKAKTDCYVGFLSSQILERLCDKYFMMYLRITETLTSLLSPRLLKLDHALEWIHLSASDTLFNQGDPANGIYVVLSGRLRQVQKTVSNQKGDETDPRGDDDEDLEILGELAQGESFGEVEVLTAMNRFSSIIAVRDSELARIPRTLFELLALEYPSIMIRVSRLVARKILASQKSGTNQPSKLADGNGGKYDLNATIPPTKATFGQRKHIHNSSTGSITFRTIAILPITSGLPVEAFAMKLVQAFKRVGRSTIGLNQRTALSHLGRHAFDRLAKLKESGYFAELEDMFQTVVYIADTPVNSSWTKTCIAQGDCILLLADATAFPEVGEYEKLLLKSRTTARTELILLHPERYVEPGLTHRWLKHRTWVDSQHHVQFAVNPLDTEANPKHNGINIGAFSLVDRFIQTEFSKKTQENISKLVPDAIKARVENISSRFINRKRQVYTPVYSHKNDFLRLARILSGKAIGLVLGGGGARGISHLGFLKAIEDQGIPIDMIGGTSIGAFVGGLYAKDYDLVPIYGRVKKFAGRISSIWRMVADLTWPVTSYTTGHEFNRGIWKTFGDVRIEDFWVQYYCNSTNITESVQEIHSSGYAWRYIRASMSLAGLLPPLEDKGSMLLDGGYLDNLPVWEMKARGCDTIFAVDVGSVDDRTPMQYGDSLNGFWIVINRWNPFSSHPNIPNMAEIQMRLGYVASVNALEKAKQTSGVIYVRPPIEDYATLDFSKFEEIYDVGADYGHTFINELIAQGKMPYIPGSQPKQEGESPEFFLRRRNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XAV2</accession>
    <name>A0A8J2XAV2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DNA polymerase epsilon catalytic subunit</fullName>
            <ecNumber evidence="17">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">BN860_00672g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">DNA polymerase II participates in chromosomal DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="17"/>
    <dbReference type="EMBL" id="HG316467">
        <property type="protein sequence ID" value="CDF91771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10060449at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05779">
        <property type="entry name" value="DNA_polB_epsilon_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05535">
        <property type="entry name" value="POLBc_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000002">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000005">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000010">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1600.10:FF:000006">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013697">
        <property type="entry name" value="DNA_pol_e_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029703">
        <property type="entry name" value="POL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055191">
        <property type="entry name" value="POL2_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054475">
        <property type="entry name" value="Znf-DPOE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08490">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22634">
        <property type="entry name" value="POL2_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22912">
        <property type="entry name" value="zf-DPOE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23250">
        <property type="entry name" value="zf_DPOE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01159">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 17" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 17" id="KW-0235">DNA replication</keyword>
    <keyword evidence="14 17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 17" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="12 17" id="KW-0408">Iron</keyword>
    <keyword evidence="13 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 17" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase epsilon catalytic subunit A C-terminal" evidence="18">
        <location>
            <begin position="1500"/>
            <end position="1893"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU365029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CDF91771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2194" mass="252850" checksum="BD67BF755436E78E" modified="2022-05-25" version="1">MSNTRFGGSSSDNYAVTAQQLTRASKIDEIDSMMGYESYIPPQHSGRIDLGNLDVVPGRVGWLNNMHPTMVTQEAVSGESDFAGGISAVDYYFLDEEGGSFKSTVTYNPYFFVKCADDTRVNEVEEFLKKYLESCIKEVNVVQKKDLKLDNHLLGLERSLIKLTFVNSNQLFEARKLLRPILTLNENNKGQKDTYTRTHPARVNGNRFEDTKLLIEDIREYDVPFHVRVSIDKDIRVGKWYKVTSAGLIEYPEKVAFADPVVLAFDIETTKAPLKFPDAAIDQIMMISYMIDGEGFLITNREIISEDIEDFEYSPTDEFLGQFTIFNEADEMCLLQRFFEHIKDVRPSVISTFNGDFFDWPFVDNRAKIHGLDLYEEIGFAPDTEGEYKSPYCSHMDCFRWVKRDSYLPQGSQGLKAVTQAKLGYNPIELDPELMTPYAFEQPQQLSEYSVSDAVATYYLYMKYVHPFIFSLCTIIPLNPDEVLRKGTGTLCEMLLTVQAYKGNILLPNKYTDPIERFYDGHLLESETYVGGHVESLEAGVFRKDIKNDFVIDPNTIDNLLEELPKALHFCIEVENKTALDEVTNLEEIEKQIKAKLLDLRNNNKRNEYPLIYHVDVASMYPNIMISNRLQPDSMKTERDCASCDFNRPGKSCDRRMPWAWRGEFFPAKMDEYNMIKRALQNETFPNPNKFSKKQHLTFDELTYAQQVGHIKKRLTDYSKKNYHRIRASEIVEREAIICQRENPFYANTVRSFRDRRYEFKGLAKKWKNKASSIGAADKHAKEEAKKMEVLYDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGITCLTGATLIQMARALVEKLGTPLELDTDGIWCTIPQSFPDEFTFTLRNGKKLLLSYPCSMLNYKVHQKFTNHQYQDLVDPVKHKYKTHSENSIFFELDGPYRAMVLPTSKEEGKGIKKRYAVFNDDGSLAELKGFELKRRGELQLIKNFQSDLFKVFLEGTTLEECYAAVGTIANRWLDILDYKGSMLEDEDLINLICENKSMSKTLKEYGGQKSTSITTAKRLGEFLGEEMVKDKGLQCKYIISAKPFNAPVTDRAIPVAIFLAEVSVKRSFLRRWTMDMSLEDFDLRNIIDWNYYRERVASVIQKIITIPAALQNVPTPVPRVEHPLWLQKKLAAEEDKFKQTSLNRFFTTTSNVPQNGKIKDIEDLFSGRDDEQGMEKVAKVSVNKKISKRKRAENKKTEESLILPSIMPSMEDDYVSWLSYQKIKWKIQARDKLRRSQLFGNSNNSMERTALGSMIRKQAESYADSTWEILQYKQSSQPGVLDVYVNINDKIQTLKFIIPKTIYVKFKRPPPVKIKHSSMERSNFVLPNSPEMKDTSSQNLYRITLPEWDYLNAIEDSASILNNENIVGVFESSIPPTERAIMELGSSVNFKSNTVGALGRGLQKGFDRKNLTITESERYLSRFSIAISYLLHIPTNIGYEFFIICNSWSDTIQVFALKPSMQSQDISATNLKQLYKEIYSRRIKKLKEYSRFFHYNEDTNFETQSFVDISKLYRKMSSQINQQKEDKGLQFLLMLQSPYVTKLLDNLRILNQVPIIEIAMTELILPQLNWRTILNKKIISNIFSCGPRLSHLVTLSQFSNIPICNLKVDTMGYIIDILYARKLQKEGMVMWWNENSPLADHGGVQRDYDPNMAGLQNDLDFPIINNPGVSEQAVLEIEVSNLTVNTVLGSALINEAEGCDLADADAENDTAARANTSNGAFVEDAFSSGSLVVLRSMLKEWWDGALKEDMIADLLVNSFVNWVQDPDSKLFDNSLRYHVHNLTKKAQLHLINEFYYLGSVVIYSDRSRLLVKTKKYSPETCYAYGQYIIKAVRANPAFVYLDLKIDRYWDILLWMDNYNFGGLACTEIQEKEKQDLKAYSQWQLKKFLPAIYQQEFDDWMMIILDSMTKTKNNWLESKLATQELVQLTTKQNNNAEDVDDGPLTNFTEIFCNPLIKRVQKLYQNQQEFILDPNFAADYAFPSLPGSHLENGNPLLELVKYLCHIMLLSKKTILEVRSLRKNLLKIFELREFDTTSEFKNPSSKLIVSSLLCEYCFFMNSINFCRGDVANFACQKCHKNFDDVLLQEHLIQKFLADVDTYLTQDVRCSRCSKIKQDSMSPHCDCSGSWEGTISKEGIEKKFKVYQQVAHFFHFQLLSNALHDMCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T0F2</accession>
    <name>A0A8J2T0F2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_14466g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_14466g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T0F2"/>
    <dbReference type="OrthoDB" id="4070718at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="74"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="546"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="245"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="546"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="585" mass="67387" checksum="41D158734CEC525F" modified="2022-05-25" version="1">MKSNKRGTSPDNHDRDMEVNSRKNVCTHEPSNKLRKIWIYPSESKLARSNRKLGQNSFFQPPTLQKIKSLSTQLNAEPLSRDDETVQNDKLNGKQASNSSMHPQKLANTNDLREKHNANNSNFLGGTLSASCELNESCLKRVKEAENIAGRNEMEESVRSILKHLTHEIMKYEMAIASLYGKIKEMDNENERLRQRYTLMCTDVVTAARQSNDLSTNDTAESTIEEECFSDGTEEEIDPIPSSNFSILKQELETLENRIVERLKELEENQRPIKQNALPNNEHEDDQISIVKELKQKLDRKESELKEVRKYAEIRVSNLKSSLISARTLQEKTNRKYEDTKERAKKASKLVSESNASIEHYKAIAHFQKDIIRSLRLQLNEKDLKISSASPKILQDNVPPTDYEERNSTNKSHDLLLEKYNRISAAYTVLSSVSNMQNNDLTILTQQYEEEKLKSLSLMSAVEQQRKISNTVRKAGPFRESDDLKKVSKMYEDLCEQQSKEAALHDEELNRVHDMYRKLLKKKDEEHHCTIENIAQELSEFRGFSEAHSLEKNEEQSSIGDNLEEDIDKSLEPVEIDSELVRITD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T0U4</accession>
    <name>A0A8J2T0U4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BN860_16204g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_16204g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T0U4"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19518">
        <property type="entry name" value="RecA-like_NVL_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19530">
        <property type="entry name" value="RecA-like_NVL_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000110">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000156">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000365">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="233"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="555"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="144"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF87863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="832" mass="92218" checksum="074C8C3FF0040D93" modified="2022-05-25" version="1">MGKNPSKKSSLRGSLDGKIENLIYRLLEEKSLEKKRSTAENEQDQDDESLEALCFAKDLKGNEIFTYCQSKDVTMLRVKKVVLQKSIERVLQQVIDEEAQEFESFPGYKDQSSLTSPLDQETLMKERNLMVVPDTNDMNKSITEQWNVKPETPNTPATPTTADDADASAVPKKRSKDIQPSKSKRQKTKEDRSPPNARLDSIGGMEDVVAQLMELIGLPILHPEIYLSTGVEPPRGVLLHGPPGCGKTSIANALAGELQVPFISLSAPSVVSGMSGESEKKIRDLFDEAKSLAPCLMFFDEIDAITPKRDGGAQREMERRIVAQLLTSMDELSLEKTGGKPVIIIGATNRPDSLDAALRRAGRFDREICLNVPNELSRVHILKKMSQTLKIDGEIDFIKLAKLTPGFVGADLKALITAAGTCAIKRIFHAYANLSAAEDPDQKMEIDQEERKELSLKNTANMIDPLPLSIIQKFIQNFPEPLDDEQLARLSIRYEDFLKALPTIQPTAKREGFATVPDVTWASVGALNKVRVELNMAIVQPIKKPELYEKVGISAPAGVLLWGPPGCGKTLLAKAVANESRANFISIKGPELLNKYVGESERAIRQVFTRARASVPCVIFFDELDALVPRRDSTLSESSSRVVNTLLTELDGLNDRRGIFVVGATNRPDMIDPAMLRPGRLDKTLFIELPNSEEKLDILRTLVKSNGTPLASDVNLETVIQDERCKNFSGADIASLVRESSVSALKRSFFKSDQVQSVIENDLDKEFEDLSVGYSSEDILVTMMDFLNALRKVRPSVSDKDRLKYEKLSNKMGWNDDVELKTDDVKNSNAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T0Y8</accession>
    <name>A0A8J2T0Y8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_16600g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_16600g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T0Y8"/>
    <dbReference type="OrthoDB" id="4068945at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="3">
        <location>
            <begin position="22"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF87881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="372" mass="42461" checksum="6A2FF4F4D939B1BE" modified="2022-05-25" version="1">MEATRRFLFTLEEARSENGVVMEIGRASRRDPSRLPCVDNLFYDERSLSRRHALLGLKLMDPVDWERNLADQFRIYVKDLGSTYGIIDLNSKESDPYIVDLKNGERFGLIQLQQPISMNQSRPAKLKFQVDVEYRGEGLFECLLTDVSFNDSPLATRPATFDERIFFRALENSSPLPYEEEPANQLESSSSSSLASTPCAHEETNEDTSWSTEEWSSEEYSRESHFSESHSSEESSSSSFVSFLIESFTKRVEELESSNQSGGVETSDKNSKPEFNRADESFSSHEGERFPESSKKEREKPEQQDINPIRTKRPLSEEHEERDQRQEKRTPPRKKTRFNIVSKRDILTGAIGVVLGSFGTIGILIGIANKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T1D3</accession>
    <name>A0A8J2T1D3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_18008g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_18008g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPC family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T1D3"/>
    <dbReference type="OrthoDB" id="300780at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000111">
        <property type="term" value="C:nucleotide-excision repair factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071942">
        <property type="term" value="C:XPC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018327">
        <property type="entry name" value="BHD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004583">
        <property type="entry name" value="DNA_repair_Rad4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018326">
        <property type="entry name" value="Rad4_beta-hairpin_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018328">
        <property type="entry name" value="Rad4_beta-hairpin_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036985">
        <property type="entry name" value="Transglutaminase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135">
        <property type="entry name" value="DNA REPAIR PROTEIN XP-C / RAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10403">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10405">
        <property type="entry name" value="BHD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01030">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01031">
        <property type="entry name" value="BHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="6">
        <location>
            <begin position="399"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="7">
        <location>
            <begin position="452"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="648" mass="77002" checksum="35F00EDCA1140E8F" modified="2022-05-25" version="1">MSKRTAEQNPRDLKRQDNLQLFYIDEEPADESDDVLLDEEEWEDVPLDGVSVTVSLPESEKHPKKKLRAHQYQRLKFGLHISLMPFMLRVLRERWMWTQDERLNRRLRRSVPKVINERFNRLFKEGNFDKLRTLLLGLVFWFRSHYQINSNGFRQNFNRLQYLIKYSNTEVEPRLYASILRNQHFFYGERPSIRDGVEDVRQMAKKKKSNRDILVVFFLIILKNLLPSNAQIKLCFALPLHDYSLACHNVKRQMNNGVGRVPDRFDTDLLAPFFWIELRFNFRPNELYVIDPVVSLKEGEIVARHAEDETVATLQPYLKINTKQKVDYIVSINCADGIMTDVSPRYLPNLYYRYFKPLESSIVLHSLGRKSYEIFIKFLGKYSKNDMPHFSLMKKLAAKNHNIPETLHEMKRSPCYIVPSLLKKNEILQSCSQHIGNFRKEPVYQKCDVLLLKSRQHWAQLGRTVKPGSTPLKSKKYVSMKKRREQRLDLYEIRELFSIEQTLPTPRLPSTYTDQSGQIRPITDVEFYSNKFGHIEIYSHDTIPNGFHLMPLRCKHVIQLFNKRCLKMGQQRIQYLEVLSGFDFRKRPGYAMPEIRNLFVNSQDYYRTVQLLQDYKQKMALQRWDELIINMKLQKRLDDTYGDLAQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T273</accession>
    <name>A0A8J2T273_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BN860_14290g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_14290g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T273"/>
    <dbReference type="OrthoDB" id="5414888at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013934">
        <property type="entry name" value="Utp13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854:SF15">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08625">
        <property type="entry name" value="Utp13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="139"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="373"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="478"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="520"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="562"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="604"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 13 C-terminal" evidence="6">
        <location>
            <begin position="657"/>
            <end position="800"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF87779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="805" mass="89490" checksum="3620534C58C9012B" modified="2022-05-25" version="1">MDLKTNYSNTALKPIYAGSGAVATVSADGKLLAAPVLDEINVVQLSPFKQILHTIENDDEQEITALKLSPDGRYLCYVSQAQLLKIFDVHLGEVARSMKISSPSYVLDSDSTSTLLAVGGTDGTITIVDIENGYVTHSLKGHGGTVSSVKFYGEQNGNVWLLASGDTNGTVKIWDLVKRRCLHTMQEHTAAVRGLDFRESSEESLVLLLSGGRDDVVNYWEFNLKKKCTLLKTLPVHQQVESCGFVNEQLIYTAGGNAVFQLISLESGKVTKKTHQPVEELFIVGVLPIANGSKMYLVYSDQTLVLIKLDQAISNAEHPIEVEACIAGNHGTIADMDFVGPNFDKLALATNSPTLRIIPRYSEGCLPIEVELYEAHSDLLNAIDTTEDGLWLATASKDNTAILWRYCFDTSRFTCYAKFEGHSAAVTAVGLPNVMPKGWPEFLITASNDLTVKKWKVPKYVENGENEMQVVKSSEYTRRAHEKDINALSVAPNDSIFATASYDKLCKIWDLETGEQKATLANHRRGLWDVAFCQYDKLLATCSGDKTIKLWSLDNFAVVKTLEGHNNAVQKCAFINKQAQLVSSGADGLLKVWDCSTGECLKTLDGHSNRIWALCTADDGDLVVSADADGVFQFWSDCTEQDRQQHDKEQVLKVEQEQSLKNYLSQGDWTNAFLLAITLDHPLRLFNVLKQSLSATLEEENSSNVIFNTELDMVISELSSDKLLLLMQRCRDWNTNARTHNIAQKTIRCVLLHHNIVSLSEIPGMVQIVDAILPYSQRHFTRVESLVEQSYILDYALIEMDRFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2N4</accession>
    <name>A0A8J2T2N4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BN860_13630g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_13630g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2N4"/>
    <dbReference type="OrthoDB" id="687730at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22695">
        <property type="entry name" value="FHA_VPS64-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051176">
        <property type="entry name" value="Cent_Immune-Sig_Mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 170 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715:SF37">
        <property type="entry name" value="LD47843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="492"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="4">
        <location>
            <begin position="110"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="125"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="450"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="418"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF87751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="518" mass="57257" checksum="C019DE79B4BC00DE" modified="2022-05-25" version="1">MTEVERNWRAPAAQAHSSSASSTGSSNRDAVGNGISPTKRTRSNSKSSGHGQKQSSSSVKDNEGKHKSDQEQLNIPVSPRNKYTHIIILKSLNETFETKFLVVPFKPESLKLGRPVVSSNNGSQNFGGIGGANGVSKQDPQQVPQVRPDNGHFDSRVLSRNHAALSCDAHTGNIYIRDLKSSNGTFVNGSRIDQNDVELKVGDVIDLGTDIDSKFEHRKISAFVEDISMIPLINDTEIENSFMNCIGEGQKEKVAGTSSALRHVNGVSGSNPEAQSMTAQRAAFDAAMFGDVNNLDLEDTVLGSETEILSGIFINNSIGTSSNLINVVKTLATEISLEKHEYTKLKSMESFLINYITNLDYVNRLMVEKNDKQLIKLQNALRQKLTEKQDAIVKEHKCQMDKIDRESKALKTSYETKERDKDIHIKRLERDLEDLRTRLEVEKYKSSQFAKKAALPEPSQPKETSDTKNEENIENGEVLNSEKPIGSSRKKMGGGTIFFVSAMSIGFVAFAMRFSSEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2U7</accession>
    <name>A0A8J2T2U7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_18184g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_18184g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the free Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2U7"/>
    <dbReference type="OrthoDB" id="15735at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033745">
        <property type="term" value="F:L-methionine-(R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.40:FF:000008">
        <property type="entry name" value="GAF domain-containing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000614">
        <property type="entry name" value="FRMsr_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051330">
        <property type="entry name" value="Phosphatase_reg/MetRdx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021:SF15">
        <property type="entry name" value="FREE METHIONINE-R-SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021">
        <property type="entry name" value="GAF/PUTATIVE CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01320">
        <property type="entry name" value="UPF0067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="2">
        <location>
            <begin position="62"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="171" mass="18815" checksum="AD429A8D6C5043B8" modified="2022-05-25" version="1">MGEHHADFANFETSGREEALALVLQHYEALTTNQDNWVCNLANASSLLWHAYHTLQIPVNWAGFYVSYGQDELVLGPFQGKVACQTIKFGKGVCGKAAAEQATQVVCDVNKEPDHIACDGETKSEIVVPVVSNGETLAVIDIDCLDYDAFGPLDQYFLERLAKLISKTCKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T317</accession>
    <name>A0A8J2T317_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1,3-beta-glucan synthase</fullName>
            <ecNumber evidence="3">2.4.1.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">1,3-beta-D-glucan-UDP glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_07272g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;3)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;3)-beta-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21476"/>
            <dbReference type="Rhea" id="RHEA-COMP:11146"/>
            <dbReference type="Rhea" id="RHEA-COMP:14303"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37671"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.34"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 48 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.34" evidence="3"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1880850at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000148">
        <property type="term" value="C:1,3-beta-D-glucan synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003843">
        <property type="term" value="F:1,3-beta-D-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006075">
        <property type="term" value="P:(1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051278">
        <property type="term" value="P:fungal-type cell wall polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026899">
        <property type="entry name" value="FKS1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056261">
        <property type="entry name" value="FKS1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003440">
        <property type="entry name" value="Glyco_trans_48_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741:SF48">
        <property type="entry name" value="1,3-BETA-GLUCAN SYNTHASE COMPONENT FKS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741">
        <property type="entry name" value="LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14288">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23605">
        <property type="entry name" value="FKS1_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02364">
        <property type="entry name" value="Glucan_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01205">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="461"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="494"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="537"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="569"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="627"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="680"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1364"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1451"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1478"/>
            <end position="1495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1566"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1608"/>
            <end position="1637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1649"/>
            <end position="1670"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1676"/>
            <end position="1701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1808"/>
            <end position="1827"/>
        </location>
    </feature>
    <feature type="domain" description="1,3-beta-glucan synthase component FKS1-like" evidence="13">
        <location>
            <begin position="304"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF88317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1882" mass="214757" checksum="69D335FD3CF7B8B8" modified="2022-05-25" version="1">MSYGQPNDNNAPYEEDMGGYAQYNQGYAEPMYDEYGQPVYPDGSYYDPNAAGGDPYAQSSIGPYDDQTMDYASGYYNQPRGPAVGENFSDFSSYGPPGTPGYDAYGGGGGAYTPSQMSFGEPGSSGASTPIYGGEGYDPQAIAMALPNEPYPAWTADNQSPVTIEQIEDIFIDLTNKLGFQRDSMRNMFDHFMTLLDSRSSRMSPEQALLSVHADYIGGDTANYKKWYFAAQLDMDDAVGFRNMQLGKLSRKAKRAKKKNKKAMEEAATNPEEAEATLNQLEGDNSLEAADFRWKAKMNELQPIEKVRQVALYLLCWGEANQVRFTSECLCFIYKCASDYLDSPLCQQRTEPMPEGDYLNRVITPLYRFIRDQVYGIVDGRFVKRERDHNKVIGYDDVNQLFWYPEGIAKIVFEDGTKLIDLPSEERYGRLGDVVWGNVFFKTFKETRTWLHLLTNFNRIWIIHVTVFWMYTAYSAPTLYTHNYQQLVNNQPLAAYRWASAALGGTLASIIQIAATICEWSFVPRKWAGAQHLSRRFWFLCLIFAVNLGPIIFVYAYDTRTIKSEAAHAVAAVMFFVAVGTFLFFAIMPLGGLFTSYMNKKTRKYVASQTFTASFAPLKGLDMWLSYLVWVTVFAAKYSESFYFLILSIRDPLRILSTMTMRCTGEYWWGAKLCRQQGKIVLGLMVATDLLLFFLDTYLWYILVNVIFSVGRSFWLGISILTPWRNIFSRLPKRIYSKILATTDMEIKYKPKVLISQVWNAIVISMYREHLLAIDHVQKLLYHQVPSEIEGKRTLRAPTFFVSQDDNNFDTEFFPRNSEAERRISFFAQSLATPIPEPLPVDNMPTFTVMTPHYAERILLSLREIIREDDQFSRVTLLEYLKQLHPVEWDCFVKDTKILAEETAAYEGNDEDMEGKEDALKSQIDDLPFYCIGFKSAAPEYTLRTRIWASLRSQTLYRTVSGFMNYSRAIKLLYRVENPEIVQMFGGNAEGLERELEKMARRKFKLLVSMQRLTKFKPHELENAEFLLRAYPDLQIAYLDEEPPENEGDEPKIFSALIDGHCELLENGRRRPKFRVQLSGNPILGDGKSDNQNHALIFYRGEYIQLIDANQDNYLEECLKIRSVLAEFEELNVEQVNPYAPELKYEEQSTNHPVAIVGAREYIFSENSGVLGDVAAGKEQTFGTLFARTLSQIGGKLHYGHPDFVNATFMTTRGGVSKAQKGLHLNEDIYAGMNALLRGGRIKHCEYYQCGKGRDLGFGTILNFTTKIGAGMGEQMLSREYYYLGTQLPIDRFLSFYFAHPGFHLNNLFIQLSLQMFMLTLVNMNALAHESIMCIYDRNKPVTDVLYPIGCYNLSPVVDWTRRYTLSIFIVFWIAFVPIVVQELIERGLWKATQRFFRHICSLSPMFEVFAGQIYSAALLSDMSVGGARYISTGRGFATARIPFSILYSRFAGSAIYMGSRSMIMLLFGTIAHWQSPLLWFWASLSSLMFSPFIFNPHQFSWQDFFLDYRDYIRWLSRGNSKYHRNSWIGYVRMSRARITGFKRKLVGDDSEKAAGDASRAHKTNIILSEIIPCAIYSAGCFVAFTFINAQTGVKATDDDRVNSTLRIIICTLGPIVVNLGILCFCAGMACCCGPLFKMCCKSSGSVMAGICHGFAVIIHIAFFIVMWVLEGFHFARMLIGVVTCIQCERLVFLCMTTFMLTREFKNDRSNTAFWTGKWYGMGFGAMAWSQPYRELCAKTIEMSEFSADFVLGHTILFAQFPVLCIPYIDKFHSIMLFWLKPSRQIRPPIYSLKQTRLRKRMVKKYSWLYYTVFCLFVACIVGPAAASSHVSKTIGSSVTGTFKNIVQPRGKDNNDTGLQMSTYVSHWYTSTPSLKTWSTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3D2</accession>
    <name>A0A8J2T3D2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Malate dehydrogenase</fullName>
            <ecNumber evidence="3 12">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_12398g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3 12"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3D2"/>
    <dbReference type="OrthoDB" id="4069699at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070013">
        <property type="term" value="C:intracellular organelle lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000268">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000009">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
        <property type="entry name" value="Malate_DH_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01772">
        <property type="entry name" value="MDH_euk_gproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF16">
        <property type="entry name" value="MALATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="13">
        <location>
            <begin position="9"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="14">
        <location>
            <begin position="166"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="15"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="134"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF87697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="356" mass="37343" checksum="4540B5D9F99F65AD" modified="2022-05-25" version="1">MPHSVNEDIKITVVGAAGGIGQSLSLLLKTQLASLLPSHRHAHLALYDVNADAVRGVTADLSHIDTGVSVSGYEGDGIGGALEHADVVLIPAGVPRKPGMTREDLLAINAKIIKSLGASIAKYCDLSKVFVLLISNPINSLVPVLVKELEAHAPGLPVERRVFGITRLDAVRASTFLHEATVRAGSTPKSNTLPYVPVIGGHSGETIVPLFSLVPGASDLSKDTLKELVHRVQYGGDEVVKAKNGAGSATLSMAHAAYKVVESFIPLLTGSVPSIEGTFYVALQDSKGQPINASAQKLLSHINGLPYFAVPLRVTSAGVDEVDAGIIERMSLFERERLLAPCLGKLQGNISTGLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3F5</accession>
    <name>A0A8J2T3F5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_12772g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_12772g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3F5"/>
    <dbReference type="OrthoDB" id="64915at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000544">
        <property type="entry name" value="YMR315W-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840:SF5">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase C-terminal" evidence="2">
        <location>
            <begin position="144"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="350" mass="38114" checksum="FAEB2354BD30540C" modified="2022-05-25" version="1">MAPILNVGIVGTGIFARDMHLPCYKSFPDEFKVVAATNRTKSKALAFAKIAGIPEDKVYDDLDEILNDKNVDYIDAMLPAQFNLPTVQKAIAAKKPIILEKPIAANLDQARELVKIAESTDVPVGIAENWLFLEVIDVAKKHLERIGPIVAFIHNSTGPFISHNKYLTTSWRQHPKHIGGFLSDGGVHQLGLVISLVGEFASVSALTKQVRPQSGTDDIVFSTVRVKDSDVIGNFTYGSAFGATEKSVFLKIFGKSGTILIDLSNKAKPVVKVRTGETQETASDEEVYPVETDGTNGVKAEFLNFHEAVAKKDKTLVRGTPRKTFHHLACVAAFLESSSKNGDSVKVQEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3K7</accession>
    <name>A0A8J2T3K7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Monopolar spindle protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MPS2</name>
        <name evidence="13" type="ORF">BN860_16842g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Component of the spindle pole body (SPB) required for insertion of the nascent SPB into the nuclear envelope and for the proper execution of spindle pole body (SPB) duplication.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Cytoskeleton</location>
            <location evidence="11">Microtubule organizing center</location>
            <location evidence="11">Spindle pole body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus membrane</location>
            <topology evidence="11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the MPS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3K7"/>
    <dbReference type="OrthoDB" id="4035046at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071988">
        <property type="term" value="P:protein localization to spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030474">
        <property type="term" value="P:spindle pole body duplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031433">
        <property type="entry name" value="Mps2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17060">
        <property type="entry name" value="MPS2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="106"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="136"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF87892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="342" mass="39284" checksum="55DEFC9FF6613B3D" modified="2022-05-25" version="1">MDLDKSSSSIVLDLAWSDVDRKNQDFIYAKDFPALIRSIEKILNRGKPEELPLLSNTGKSVIDTFAKEKEFFKIYRDEFKEIFHGLVGKTFKDAIEMANAPGQLSAGSVLQDTQPSKPQEAVKSNKSSPRKANRLLRNLENRVSTMKEELKFKDDILAEKDRELIQLTRKLGEYKDKYEFVQRQFNFYKDHGESPKRSSSESSNMEQNASTKHEFIISELKRKLQEQTLAISTLREQLHRTEGAGAFYSKSAKDMNLNDWPKAMTAFLLLASLVILLFGGLLWLTSGSEDSTSFAQPSWWEKNGVLSRIGWFIRDWSTNSVDYVDFEPSSDAYDRIMGIRRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3Q7</accession>
    <name>A0A8J2T3Q7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosylphosphatidylinositol anchor biosynthesis protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_17568g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGF family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3Q7"/>
    <dbReference type="OrthoDB" id="17366at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009580">
        <property type="entry name" value="GPI_biosynthesis_protein_Pig-F"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06699">
        <property type="entry name" value="PIG-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="99"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF87924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="215" mass="24765" checksum="772390317BD34B8F" modified="2022-05-25" version="1">MGFKNKRGSVKKSVSFSDDNTLTRNTLKRHIDHDNPPVYVRTTTLTVPWHLFLLLYYYIYVSTNYDTVRMLYLLIPLQIFYLVFQFNKSTVYGKKLLKINIPLVLVSLSASALLTLPTMIIIILFGAPMTDKLIETWLLSLHLCFLAYPAVYQVFNGDFKVGLWKKYFIMIVVGAWTSCIVIPLDWDRYWQTWPIPVVVGGYLGALIGYSIGAFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3Q8</accession>
    <name>A0A8J2T3Q8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BN860_12728g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_12728g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3Q8"/>
    <dbReference type="OrthoDB" id="201153at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13970">
        <property type="entry name" value="ABC1_ADCK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034646">
        <property type="entry name" value="ADCK3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051409">
        <property type="entry name" value="Atypical_kinase_ADCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851:SF3">
        <property type="entry name" value="COENZYME Q8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="6">
        <location>
            <begin position="200"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="67"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="70"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF88544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="544" mass="61816" checksum="9B7319661B70F18B" modified="2022-05-25" version="1">MSSRRSLYYVYCLGSSARQVLGGSWDVGKESLITWINTSTLTRPILSQYQWFYDPEWENARKLAEETRVARHGQRNKRNQPKKAGIRQYSTSTKAPANEDSEKEKRELQSSEVPSSRLARLFHYGSLAAGVGINVASEGISRVAKGKTPTWRELILSQSNVERITKKFSQMRGAALKVGQIMSFQDDKVLPKELYEVLSRVQNSAHYMPQRQLNKVLVTEFGADWASKFAHFDKIPIAAASIGQVHKAELPTGEKVVVKIQYPGVKDSIDSDLNNIMMILGASRLLPKGLFLDKTIANARRELKWECDYVREAAALRQFEDLLKDDPVFEVPHVYRELTTNNVITMSYMEGTEIMKLPKSTAQDLKDHIAGNILRLCLQEIAVFKCMQTDPNWANFLYNSKTNKIELLDFGASRSFPESFITNYRKLLSYATEQNREMVGKLSKELGYLNGLESQAMIDAHVNSVMILGEPFAGPPDAAFSFKDQTVSDRIRSNIGLMLDERLCPPPEETYSLHRKFSGIFLLCARMEATVFCSKYFHEFFYLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3S4</accession>
    <name>A0A8J2T3S4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="17">ATP-PFK</shortName>
            <shortName evidence="17">Phosphofructokinase</shortName>
            <ecNumber evidence="17">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">BN860_15588g</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 17">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="17">Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 17 18">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 17">Heterooctamer of 4 alpha and 4 beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 17">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Mitochondrion outer membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="17"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3S4"/>
    <dbReference type="OrthoDB" id="537915at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000010">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000007">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000008">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03184">
        <property type="entry name" value="Phosphofructokinase_I_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009161">
        <property type="entry name" value="6-Pfructokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040712">
        <property type="entry name" value="Pfk_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF57">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18468">
        <property type="entry name" value="Pfk_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000533">
        <property type="entry name" value="ATP_PFK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="13 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 17" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15 17 18" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="12 17 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="14 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase N-terminal" evidence="21">
        <location>
            <begin position="10"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="20">
        <location>
            <begin position="205"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="20">
        <location>
            <begin position="593"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal catalytic PFK domain 1" evidence="17">
        <location>
            <begin position="1"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interdomain linker" evidence="17">
        <location>
            <begin position="579"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal regulatory PFK domain 2" evidence="17">
        <location>
            <begin position="593"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="168"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="354"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="276"/>
            <end position="277"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="306"/>
            <end position="309"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="352"/>
            <end position="354"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="396"/>
            <end position="398"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <position position="453"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="486"/>
            <end position="489"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <position position="663"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="720"/>
            <end position="724"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="758"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="765"/>
            <end position="767"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <position position="825"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="851"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="857"/>
            <end position="860"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <position position="949"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR000533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF18468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CDF87837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="985" mass="108451" checksum="AD6F1FFD1752474E" modified="2022-05-25" version="1">MVLAHEACYGVAFRSVVTDSEKLFHDTVHFYHTLGFSTVKDFDKFKHGENILLCSGCCQDSLREMWLEAFKLSEIDGNGFRIPQQEAGNKHQSQGALLKVRLVMGELLKEDSRDTAVTYFSTELDEVLKRFPQAEKISDTLIQLVDPLGNLLSFTSFANALDTDPTPKEYFLDGPRKSDGGEDASDSDAVKQLKNSLALSSKKKKIAVMTSGGDSPGMNAAVRAVVRAGIHYGCDVYAVYEGYEGLLRGGKYLRQMHWEDVRGWLSEGGTLIGTARCKEFRERWGRKQGAYNLISEGIDALIVCGGDGSLTGADLFRSEWPSLVEELVQDGKFTIEQVEPYRNLTIVGLIGSIDNDMAGSDSTIGAFSALARICELVDYIDETAKSHSRAFVIEVMGRHCGWLALLAGIATGADYIFIPERAAPKGKWQEELKEVCQRHRAKGRRNNTVIVAEGALDDELNHIGAEDVKNVLLELGLDAKVTILGHVQRGGTAVAHDRWLATLQGVDAVNAVLEMTPETPSPLIGILENKIIRKPLMESVKLTKSVATDIENKNFDKAIMLRDTEFVELYENFVATTIKDDGSEMLPEAQRLNIGIVHVGAPSAALNPATRAATLYCLSRGHRPFAILNGFSGLIQTGQMQELAWADVESWHSLGGSVIGTNRSLADEDMGTVAYYFQKKKLDGLIILGGFEGFKSLKQLRDSREQYPIFNIPMVLIPATISNNVPGTEYSLGVDTCLNALVKYTDEIKQSASASRRRVFVVEVQGGHSGYIASFTGLITGAVSVYTPEDKINLATIKEDLELLCENFRHDRGENHNGKLLIRNEQASSLYSTELLANIIAESSKGKFGVRTAIPGHVQQGGIPSSKDRVIASRYAVKCVNFLEDWNERNKGVDADTKVLRFKYDIHGEKRPTVQHEDDSAAVICVNGSKLSFKPIAELWENETNVKLRKGEDIHWKEFTRIGDILSGRLRLRAEVAASAGSKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3W1</accession>
    <name>A0A8J2T3W1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">General transcription and DNA repair factor IIH</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_08988g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the GTF2H2 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3W1"/>
    <dbReference type="OrthoDB" id="284275at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01453">
        <property type="entry name" value="vWA_transcription_factor_IIH_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000477">
        <property type="entry name" value="General transcription and DNA repair factor IIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000015">
        <property type="entry name" value="General transcription factor IIH subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007198">
        <property type="entry name" value="Ssl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004595">
        <property type="entry name" value="TFIIH_C1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012170">
        <property type="entry name" value="TFIIH_SSL1/p44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00622">
        <property type="entry name" value="ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07975">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04056">
        <property type="entry name" value="Ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015919">
        <property type="entry name" value="TFIIH_SSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01047">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="137"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="13">
        <location>
            <begin position="361"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR015919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR015919-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF88385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="473" mass="53220" checksum="40A2B331B545F975" modified="2022-05-25" version="1">MPPMIDPESDEDEEHVQVSSKETRRQKRQFDHDEENDEEGRKRGGSRPSSSKHRNGRSSKESARHTRSKRRNKTSNKALQGASGGYAWEDEIKRSWDLVTVDDEGDMAALAASIIEARKKRTAQKNITPYQRGIIRTLILVLDCSEAMLEKDMRPNRHAMLVQYSIDFVHEFFDQNPISQIGIVSMRNGLAHMISQVSGNPQDHVDALKSIRKQEPKGNPSLQNALAMSRGLLLPVPPHCTKEVLVIFGALSSTDPGDIHLTIGSLVQDGIRVKIIGLSAQVAICRELCKATNYGDESFYKVPLDETHCKELFDEAVTPLPVNKINKGFTLVKMGFPTRIFEEAPTFCACHSKLLYGGYFCPNCQSKVCSLPMVCPCCDLMLILSTHLARSYHHLMPLKTFSEVPTQETFPTENCFSCQARFPMIKNHKSGELLTSSRYRCEDCRQDFCIDCDVFIHENLHNCPGCESQPTIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T458</accession>
    <name>A0A8J2T458_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BN860_18118g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_18118g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP4 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T458"/>
    <dbReference type="OrthoDB" id="1650at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071034">
        <property type="term" value="P:CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22525">
        <property type="entry name" value="KH-I_Rrp4_eukar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05789">
        <property type="entry name" value="S1_Rrp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000063">
        <property type="entry name" value="Exosome complex component RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000038">
        <property type="entry name" value="Exosome complex component RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025721">
        <property type="entry name" value="Exosome_cplx_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026699">
        <property type="entry name" value="Exosome_RNA_bind1/RRP40/RRP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048565">
        <property type="entry name" value="S1_RRP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321:SF4">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321">
        <property type="entry name" value="PNAS-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14382">
        <property type="entry name" value="ECR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15985">
        <property type="entry name" value="KH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21266">
        <property type="entry name" value="S1_RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0271">Exosome</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exosome complex component N-terminal" evidence="7">
        <location>
            <begin position="53"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="RRP4 S1" evidence="9">
        <location>
            <begin position="102"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="196"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF15985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF87949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="359" mass="39678" checksum="AB952A3814F6FA7D" modified="2022-05-25" version="1">MSSVISIAKRKGVFQTSIDSGFELRDDDEDISMSDANEDIQSEHEHNASERHIVTPGELVTDDPVWMRGHGTYFLDNMTYSSVAGTVSRVNRLLSVIPLKSRYSPETGDHIVGRIAEVGNKRWKVDIGGKQHAVLMLGSVNLPGGILRRKSESDELQMRSFLKEGDLLNAEVQSLFQDGSASLHTRSLKYGKLRNGQFCQVPSSLIIRSKNHTHNLPGNVTVVLGVNGYIWLRKTSTMDMARDAPSGADAIKGAPIGTKTLRSSDRVSITRLEEESSWQIYSDENDPTISVSLRRTISRYANVIKALAYCEIGITQDRIVGAYEASMVYQNVGSLIEREIMETIGQDVLNAEKMRGSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T476</accession>
    <name>A0A8J2T476_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_11936g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_11936g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T476"/>
    <dbReference type="OrthoDB" id="270639at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016150">
        <property type="term" value="F:translation release factor activity, codon nonspecific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070126">
        <property type="term" value="P:mitochondrial translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052104">
        <property type="entry name" value="Mito_Release_Factor_mL62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11075:SF54">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11075">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110916">
        <property type="entry name" value="Peptidyl-tRNA hydrolase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="1">
        <location>
            <begin position="36"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="168" mass="19836" checksum="B48986CF3C609F42" modified="2022-05-25" version="1">MRPCQVWFTAESPKHLKLAKNWVVSLNSKRVPLNLFAVRYDRSSGPGGQNVNKVNSKCTIALHNFSRCSWFPLEVRKQLSEKKSRYYASSSDTIVIQSSESRSRETNREYCIEKFVKHIKEICFFPGEADEHTVKKWHGIKEKAHERRIQEKKLQSNKKRLRNKKDLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4A4</accession>
    <name>A0A8J2T4A4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sister chromatid cohesion protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_13036g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the SCC2/Nipped-B family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="418242at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090694">
        <property type="term" value="C:Scc2-Scc4 cohesin loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061775">
        <property type="term" value="F:cohesin loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140588">
        <property type="term" value="P:chromatin looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034087">
        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071169">
        <property type="term" value="P:establishment of protein localization to chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23958">
        <property type="entry name" value="SCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026003">
        <property type="entry name" value="Cohesin_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024986">
        <property type="entry name" value="Nipped-B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033031">
        <property type="entry name" value="Scc2/Nipped-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704:SF18">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN DELANGIN SCC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12765">
        <property type="entry name" value="Cohesin_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12830">
        <property type="entry name" value="Nipped-B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Sister chromatid cohesion C-terminal" evidence="7">
        <location>
            <begin position="1188"/>
            <end position="1367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1469" mass="167370" checksum="FAF83705469372C2" modified="2022-05-25" version="1">MSSFPGENTDIPKRLLEALESQPLNHLIPKAGLFDIIGTTVRASMPSDRSAFSTPLQEVLNQSRGINLDPRKSKYVFRRPKNNITMAQSSSTKKIIEDLSPLAKECLAATGCEVNHEDIKFRLESQENHNLKHKLENNDTIPLNFNRDTKMKKVKIESSVTVNQAALAKRYIEDLIIFLDEVVKDFSAEWRNIEHWYSLPNKNFVLTETCIAKLQISMNNVLATPTANTTTTIERLVKLLDILVLNIKISEEFGTNDSQKDLLNRVAHASVSLIFTIFLLAGDDKRISLEHYLLTPLDFVETSFKKIKHLLSDQESLKNELNLLHQSILLLPLYINRRPFLDKGIITKLIYTFTDFIMENGIDVVNNSLSLQYCKDKVTSMSIDILVSLFAKLPDQRELIIEELLSNVEKLPTKRIQKKLKKLDNEIYVTDFTVAILLFLQNINCYHYAKTISVSDNGFVTAVAEKHIEETRILNAFASHLTESIFDRFVKSTSGYRHVIENYVLDLLALLPYPQWCVAQDLLTSMTRKLLLAFDPSHPHSTSVETICLHLLGSIGLSIFEIKCSTNPENANNLVKLFNSPNHLPFFLKSFEKCSEYLCGLGKQSANRYLWHKKLAILLKLDEYGTDAQAEGKSNLSYQEIKNLVEQNPNHSKTMTKLNVSEVRNDYFSILHALELLQLYEPYLKLILSILGKDKVKLKSTAIKCLSLIANEDQNVLKTPMVKNTIGRTLKDSSASVKAAVLDLISIGSSYLQYYQQLNINYDDESISIRKQVLKINGSIYDETDSLDVKIFVASKILSKIEDEEDNIIEIARNMLLERWILSMKDEESLLDQCSDLCNDILSVMSNIATLNSKCSQLFECYLNFYLLNEQMHSQNTYERIMKSFRKLTDHLVQHVVELQSMEVHEKTNSTKQKLLILLSKFSDCSATFITKDHIIALYPYIASDEKSEWQFHILRVFNRTMQHLSNFKPKFLCDLETTLLSRLPRMNVKEIGEAMPLIWCIATSRKDTGRVTKACSSCFQHLTPYINQANKNPGSITVESKLQRLIYLSAAFARFCKFPRKESKPIHVQDDEPVHEYVAKCLLLLSRPGTPHVIRRISIKALTQLCGDHPKLFNSKHILNLLDEEFESTNMDIKLVILESFYDFFVAEEHKSLKQSGVNVSISSHVASGKQSSKKNKKESISGDGICAALVTRFLKHLLKICLSPDIRSSLVAVRLLKLILQYGYTNPSHCIPTVIALTGSTNRYLHAIGTEVLNELFEKYETMVFAGISQGVKITVEYLKTLEENDYYKNNTFLKNLQIIMGNSKKNSTKFFKCLTRILKLYFSQIVASGTESETKEGILFLTNNISELKFSSQYELMCLLKVIDLESAQLEDAISDNLEQKEESVQLDKKVRNNIAIQFSLQALKEFFLSLYGLKPDLIGEVEESELRIKLLPPISAQSRFGEKLNIIIAQADKHNPNDLSAKELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4J0</accession>
    <name>A0A8J2T4J0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_13542g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_13542g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4J0"/>
    <dbReference type="OrthoDB" id="247245at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02022">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00376">
        <property type="entry name" value="Dephospho_CoA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00152">
        <property type="entry name" value="dephospho-CoA kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695:SF46">
        <property type="entry name" value="BIFUNCTIONAL COENZYME A SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695">
        <property type="entry name" value="DEPHOSPHO-COA KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51219">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="238" mass="26716" checksum="B72B2FD91781FC49" modified="2022-05-25" version="1">MLVVGLTGGIASGKSTVSRRLEEHHHIPIIDADKIAREVVEPGQAAYTKIVNYFQSKIPNLLLDDNHLNRAALGKWVFSNSEDLQVLNQITHPAVRYTIFKRVLFCYLRGYSMCVLDVPLLFEAGLDLFCGVTIAVLSEPSLQLERLLARNPQMSNGEAKSRINSQTTNEERIEKADFVLTNNGGLPFLYREIDELVRRIQPTVLRTVLEYIPPFGAISAAAIVLSRYEAFAQTQKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4K2</accession>
    <name>A0A8J2T4K2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_16996g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4K2"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045143">
        <property type="term" value="P:homologous chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000635">
        <property type="entry name" value="Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="9"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="309"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="403"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="406"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="470"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF87899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="481" mass="54907" checksum="029ED70AFF8545EE" modified="2022-05-25" version="1">MDLRVGRKFRIGRKIGSGSFGDIYHGTNLISGEEVAIKLESVRSRHPQLDYESRVYKYLSGGVGIPFIRWFGREGEYNAMVIDLLGPSLEDLFNYCHRKFTFKTVIMLALQMICRIQYIHGRSFIHRDIKPDNFLMGVGRRGSTVHVIDFGLSKKYRDFNTHRHIPYRENKSLTGTARYASVNTHLGIEQSRRDDLESLGYVLIYFCKGSLPWQGLKATTKKQKYDRILEKKLCTSVETLCAGLPQEFAEYMLYCRNLKFDERPDYLYLARLFKDLSIKLEYHNDHLFDWTMLRYTKAMVEKQRDLLLDTPQDNTNNDNNNSSNINNANNSSNNNGKGDSFNKVKLLAMKKFATHFHYCKNEDKHHPTPEEIKQQSVQNSNAAALLPEELLNAIDKGMESLKNQHATAPAPAPAQAAVAGTAAGGGITTPVGAAPEPLLQQQQKESQYQPQQVPQRMTHYPPQTQPAQSKQDKPRGEDIWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4L8</accession>
    <name>A0A8J2T4L8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BN860_05204g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_05204g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4L8"/>
    <dbReference type="OrthoDB" id="2503993at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005621">
        <property type="term" value="C:cellular bud scar"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902929">
        <property type="term" value="C:plasma membrane of growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000282">
        <property type="term" value="P:cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024982">
        <property type="entry name" value="Rax2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048266">
        <property type="entry name" value="Rax2-like_second"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048265">
        <property type="entry name" value="Rax2-like_third"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31778">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN RAX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31778:SF2">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN RAX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12768">
        <property type="entry name" value="Rax2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20842">
        <property type="entry name" value="Rax2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20843">
        <property type="entry name" value="Rax2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035194159" evidence="3">
        <location>
            <begin position="17"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1160"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="domain" description="Rax2-like second" evidence="5">
        <location>
            <begin position="247"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Rax2-like third" evidence="6">
        <location>
            <begin position="422"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="Rax2-like C-terminal" evidence="4">
        <location>
            <begin position="867"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1116"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1116"/>
            <end position="1147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF20842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF87386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1216" mass="133510" checksum="4224B080CBB1F91A" modified="2022-05-25" version="1">MLSVVPLYFLLAICSASHLLDLQRQFNSSDVDISHLGLSSNDNNALELLGGCQDFSFYRYTGQENFTRPLSSTTDSKGLIYYSNNTFLQLANGFNDTEIKHIVALGRDSFILGGTGRLEGHELENQLLYNLTDNTFKPIFRHGLTNINSILVDGSLVYFGGDFSYSNESDTGHSAILWDSEQNSTSLLPFLGFGKNSSVNSIVKLDNDNILFAGKFYELDKASLLENQPLSNSSRSSNWTDVELGLAIPLQNANWTYGQSKFNTSGFVCPNSAEESWLQYGTSGSLSCTLPQESTPNKVRIYNSPIGSDEISLFRIITSPSQGIMNLTYVDPETGETRYCDAFCPLYNREKLKQTSANSSSAQHMTTFTKNNTTDIKWGRDFQEFAFVNDIAISSVEFFALSSYGENVGLSSFQLFQSSPSIYANNSLNKPACDSMKSYSEAKLSNNDWKQGLDGQTYLSTEYIPGKGDKPKVVFYPHIQYPGDYIITLYTPGCLGDGTCSQRAIVNVTLWDGDTNEPLSTGTVYENNNELKYNELYSGHLNSSPRVTLEYYSSIYPNNPSSVVVADYISIVAKSIDNFRKSNLKNITLNGLFQYQLSNFTHKTLINHTEVGNTPLNQFPLANFAPNSSIFASLYDNTTLLLADANTSAIKVKLDKEWNITSSAKYETDMQIKGIGSYSKGLVLFGSDNISANHSTALSFDGGFTSLDKINKSIESFVNLTLHESELIVFGNEFVYNVSSGGYTSNSTIFSLSVWSAGQNAYGDLIFSGAVSTNDFSTLNGPFHIFQNGSLKSVNNLGEHFNPYLGTHLNDTLTAYAYKDGSHSSLVFSNAIKGDWEWPHLIQSMLYSNKDTVLAIGTASSSYSSQLTLLNLTTFKMLANETFNEGTDVSTMILFDKDATLLVGGSFSIPEARCHGLCLFNYKSKTWSSFANGTINGNVSEIQLHNTTELLISGSFKVQNSSNVNLASLQITGERIRPLLAGWKEPLKSFIVSGNTLIAWNATNLVTYDNSSWKDVPIPISDSSPGIKDVKDLQLQQSQTKRDLSSSGSKALLVYGAFREEQNNAYQGLIYDYDSWEPFFMVNFKGSDSNSKPNLFMNKNFSDLYISEETLLNQNTTRTSSLRSSSTTLPPLSKTSTASSIASSSPSDKAEHHHKVDRGFVVLIGLALAVGTVAAIGIIGVLVAYIFGADIGEYEFLNPPSGIGSVNGTAPPEKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4N3</accession>
    <name>A0A8J2T4N3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BN860_09626g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_09626g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4N3"/>
    <dbReference type="OrthoDB" id="1684102at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF530">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="363"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="401"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="431"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="479"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="524"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="548"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="580"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="9">
        <location>
            <begin position="215"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF88410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="609" mass="67512" checksum="4C71EEB1C89F94CB" modified="2022-05-25" version="1">MDSSNVSQSLAKGQSNGKDRSAIDIHNPDPQVVDIVSRHLVNSPNNLQLQGGDITRDLYKWSNEQQQLPSHPQLGTSPQLSQHKDDLHASLGQRRRSLSFSGMSAHSSSTHNEPHGEVPGTRMTHEEIRAPGGFRRSFILQQHERKYGATAPPPNFFARNFIEFLTLYGHFAGQDLEEDEDEEEDEEQEALRNQDEESVLLERVSTRDSRRKQRKATTFKAVLLLLKSFVGTGVLFLPRAFHNGGWAFSTACLLFCALASSLCFILLISAKGKVGVNGYGDLGKALYGDKMRFMILFSIALSQVGFSAAYTVFTATNLKVFSENALAVPVDSVPLSAYIVFQALIFIPLSLTRNIAKLSGTALVADLFILLGLLYVYYYPAFLIAKHGVSDSILLFNRTDWSLFIGTAIFTFEGIGLLIPIQESMKHPQHFYPSLAFVMCIVTLIFISSGLLCYCAFGAKVETVVLLNFPQDSIATMSVQLIYSLAILLSTPLQLFPAIRILETWTFPLNASGKHNKRVKWLKNYFRTGIVILTALLAWIGANDLDKFVSFVGSFACIPLIYVYPPLLHLKVFGIGGSKLIVLGDALILVFGMGIMAYTSYQNIVLWGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4N8</accession>
    <name>A0A8J2T4N8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_18404g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_18404g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4N8"/>
    <dbReference type="OrthoDB" id="4032461at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="438" mass="51494" checksum="61E149D4A947B351" modified="2022-05-25" version="1">MLSSENDNREQCTCSCGFSHQKSKDRRTNTLSKRNSSRPRNTRTRPKFRFTRCLQFQGTDYNLITSVDYLNEKYGIRKSHYIEKFIKCIHKKIDHDVNLITDEYVDSLGPWVKIKFFLLLVTLSQRGGPEYWMDKDHVPVNAEGTSSDKSNRESSPTHNNQFHNNHNNHNCHKKMINERGDVIFSLTEQIMRQNINQDFTESTYDENYVFSSIWANFMEGLINHYLEQVIVPHSEMKVCQQLYKPMMKIISLYNEYNELMEKSEHNGFLPSPGDSQNPFKLGKSVSEAYEIGDCVTQERLQRAQKLLWQARQDIPKTISKELTLLSEMYSTLSADEQDYELDEFVCCAEEYVEVEYLPSLVEVLFQNCGSMHFWKIMVVLEPFFYYIEDVGDEEEAEEQESSNEELIDDNYMSQSFKPDPRVIILEKICEVAARQKWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4T8</accession>
    <name>A0A8J2T4T8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein BFR2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_17480g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AATF family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4T8"/>
    <dbReference type="OrthoDB" id="5783963at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025160">
        <property type="entry name" value="AATF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039223">
        <property type="entry name" value="AATF/Bfr2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012617">
        <property type="entry name" value="AATF_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565">
        <property type="entry name" value="AATF PROTEIN APOPTOSIS ANTAGONIZING TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565:SF0">
        <property type="entry name" value="PROTEIN AATF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13339">
        <property type="entry name" value="AATF-Che1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08164">
        <property type="entry name" value="TRAUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AATF leucine zipper-containing" evidence="5">
        <location>
            <begin position="167"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Apoptosis-antagonizing transcription factor C-terminal" evidence="4">
        <location>
            <begin position="394"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="100"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF87920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="506" mass="57290" checksum="594A846591B2CE9B" modified="2022-05-25" version="1">MGRLLGDEIAEEANKPVMEDYDVEDVFEHQETDSNDESVSGDEELGKAHYTPVEKSRLRKEVFELDGKYQGTIGSRDSAFGESDAGEKEESDAVSFRTDSEDESVSEDDREHEDEREEEGEAEPGDEENEDAKNSQEEELQRARLAELIQAERKKAASNLSQSTQRDASKGFAILEQSKLFDNVIDVRMKLQKAVAEVNKLPLSKESWKKCESAETSKLLKQTVKLLNKTLAQVTDFRREFQVEEEITDADSSRSNGKHKRNFKELEAECNELDDQLRPYRSAVLHKWSSKIAAASGNAALNNSKFKAINQPANVQVENQLADLPRLLKRTRLSRKGVLPLCFEQDLSSGRLAKAEAQATNDEEEVDIPKNYDPRRKGFDVQDNAYIFDDEDFYRVLLNDLVDKKINNARNQGTGAQIAITTRSNKLKKDVDTKASKGRKLNYAVQEPIANYEAPKTSANQWSDEQIDEFFAGLLGQKVNFDEDRITEEQPEEDEAIKNDNIQIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4U2</accession>
    <name>A0A8J2T4U2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="11">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_04346g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="11"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000729">
        <property type="term" value="P:DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17920">
        <property type="entry name" value="DEXHc_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18794">
        <property type="entry name" value="SF2_C_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000668">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000296">
        <property type="entry name" value="ATP-dependent DNA helicase RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000340">
        <property type="entry name" value="Bloom syndrome, RecQ helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022758">
        <property type="entry name" value="Helicase_Sgs1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002121">
        <property type="entry name" value="HRDC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032284">
        <property type="entry name" value="RecQ_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018982">
        <property type="entry name" value="RQC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF153">
        <property type="entry name" value="RECQ-LIKE DNA HELICASE BLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11408">
        <property type="entry name" value="Helicase_Sgs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16124">
        <property type="entry name" value="RecQ_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09382">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00341">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00956">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="562"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="761"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="248"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="302"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1261"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="165"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF88190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1292" mass="146260" checksum="CD17E8DB76D7863D" modified="2022-05-25" version="1">MVTKPSNNLRSECKWLKETKCFQDDRDLVLRIINSNTLNKRSRTASCIAVQKKSNVQTNELKAPGCETFDVTGPTIPSPDTHPKSVLSQQKNSPGLVTVPWSLIQCLNLKSELLSQKCSIFESTSLSEDAKRSQISNKINPELLSLESKLEVLQKDIRIIKDSKYSSSDNSHSSILPSPEDGNLRGDLLASASQQFLRTTQPPIPNSLSVPIEVPVEIPGFSSAAAKIQDDLIQVLDDDELEPVVLSQKKEQPTMTPQSQHDTQEGAQQYNRSRSLRARDDINYRIPEHNDPFDYVMGVTEPYSGASDGTMEAEDDNSSNYLHTDDENDLQPNQSDIDFVVMDEGGDTIDQSYGEVPNAATQSDVEANRSHEIDSDSIEMILSSPQGKFSSRQHNVEHIDLIEDEFEKDQIIEDTLASPSRNFTMSHSDLELIVSEGEGEDGVTISESDLEKFDEERENRARVNDIKELDDDLRIINERKLREDNFFPQSILVKQEQPSQSNFGNHHSMESLEDDSLLTNIAINEPYSAKNRRRFPWSDEVEYQLSQVFKLKNFRPNQLQAIDATLAGKDVFVLMPTGGGKSLCYQLPAIVKSGKTKGTTVVISPLISLMQDQVEHLLDKNIKASMFSSRGTADQRRQTFNLFIHGLLDLIYISPEMISTSEQCKRGIKKLYNDGKLARIVVDEAHCVSNWGHDFRPDYKELKYFKREYPDVPMMALTATASEQVRLDIIHNLELNEPLFLKQSFNRTNLYYEVLKKTKNTIGEICDSIKNRFQNQTGIIYCHSKKSCEQTAAQIQRSGIKCTFYHAGMEPNERLTVQKAWQSGKLQVICATVAFGMGIDKPDVRFVYHFTVPRTLEGYYQETGRAGRDGTPSYCITYFSFRDVRSIQTMIQKDKNLDRESKEKHLNKLQQVMAYCDNVTDCRRKLVLSYFNEEFDAKQCHKNCDNCCNSVNVVTEERDVTEESKKIVKLVHSVQDSRVTLIYCQDLFKGSNSSKIVQAGHTELEFHGAGKSMLKSDVERIFFHLITKRILQEYAIMNNSGFAVNYVKLGPNGRKLLDGKIDVRMQFNISAPGSRSKSAGATTSVATRKETKANSISLNLENFKYKDMEETVQPISFHNDTELKSTQELAELTHAYETLKELSLNAGYRLNPPVANFIPDVALKRLATALPMTEEGFTNLPDIGEKHGKKFKLFKKTIIELRKRRNDLGRNSSDCLNATNNTVVSESSFVGENVTRSRFFSIDSEEQIASAEIVRQIRESQLNSIAEPPKPKPAKSGRGRYSSNFRFKKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4Y4</accession>
    <name>A0A8J2T4Y4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BN860_05028g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_05028g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4Y4"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18626">
        <property type="entry name" value="CD_eEF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000076">
        <property type="entry name" value="Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.990:FF:000002">
        <property type="entry name" value="mRNA export factor elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000193">
        <property type="entry name" value="Probable Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015688">
        <property type="entry name" value="eEF3_ABC2_chromodomain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047038">
        <property type="entry name" value="eEF3_chromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056891">
        <property type="entry name" value="NEW1_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF14">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24988">
        <property type="entry name" value="NEW1_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="550"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="792"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="8">
        <location>
            <begin position="922"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1138"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="99"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1140"/>
            <end position="1150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF88221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1180" mass="132037" checksum="418CF61C8F18779A" modified="2022-05-25" version="1">MPPKKFKDLSEFLDEQPQEPGFVASPYGGYFKNTNNYQQQRSYDQVGKNSKQRGSNQYSNYYNQNIANYQNGASNYQNYGGYSNYQYQQSAVTPDQSKTRTPSGSTTSLTSLNENMASLQVSPVSAALGQLPQCKSIAECKKNLDIVVEEFSKEGASAKDIKTWSIPDVLTKFSKQKNPPLVKESSMLLISKLAQFFHGKHPQEAYLLPLFDYALDGMADKENTVKRAAQQALDALVNVYPVESLTSAVLPIILKYLASGAKWQCKVAALSVVDRIREDSPSDLLELTFKQTVPVLTDVATDFKPELAKRGYETLLNYVSILDNLDLAPRYKLIVDTLQDPTKVPQCVRALSSVTFVAEVTEPALSLLVPILNKSLNLSSSSQEQLRQTVIVVENLTRLVNNRIEIESFIPLLLPGVQKVVDTASLPEVRELAGKALKVLVEDQEPSKADYFPGRINFAKGQEFLQQKLPQLSIDNNCVLKIMLGDQQTIEYLARLLTVDANVNDWKRLDEYLVSVFGESNKEAIEGHIINDLRAIFHQEKTQHDESEGVEIVNTDFSLAYGSRMLLNKTTLRLLKGHRYGLCGRNGAGKSTLMRAISKGQLEGFPDKNELRTCFVEHRLQGEEGDMDLVSFIALDEELQQTSREEISEALATVGFDENRRAQTVGSLSGGWKMKLELARAMLQKADILLLDEPTNHLDVANVKWLEEYLLEHTEITSLIVSHDSGFLDAVCTDIIHYENKKLAYYKGNLAKFVEQKPEAKAYYTLSDSNAQMHFPPPGILTGVKSNTRAVAKMNHVTFTYPGAPRPSLHDASCALSLSSRVAVLGPNGAGKSTLIKILTGELVPQEGEVQKHPNLRIGYIAQHALQHVNEHKEKTANQYLQWRYQFGEDREVLLKESRKVSEEEKEILKKEIDIGDGYSARAIEAIVGRQKLKRSFQYEIKWKLWKPKYNSWVSKEKLVEEGFGKLVQKFDDFEASREGLGYRELIPSVITKHFEGVGLDAEIANHTPLGSLSGGQLVKVVIAGAMWNNPHLLVLDEPTNYLDRDSLGAMAVAIRDWSGGVVMISHNNEFVGALCPEQWIVEDGRLTQKGVQQVDQSKFEDGGNANAVGLKNLHAASPSVTDDDSPANIKVKRKVKRLTRNEKKAQTERRRLRYIEWLSSPKGTPKPKNSDDEESDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T559</accession>
    <name>A0A8J2T559_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phospholipase</fullName>
            <ecNumber evidence="7">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_11672g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the phospholipase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="7"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01254">
        <property type="entry name" value="PH_PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09138">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09141">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000011">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016555">
        <property type="entry name" value="PLipase_D_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF76">
        <property type="entry name" value="PHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009376">
        <property type="entry name" value="Phospholipase_D_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PH" evidence="9">
        <location>
            <begin position="469"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="10">
        <location>
            <begin position="732"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="10">
        <location>
            <begin position="1032"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="70"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="142"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="226"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="356"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1176"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1481"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="89"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="149"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="257"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="356"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1177"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1486"/>
            <end position="1508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR009376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF89206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1622" mass="188022" checksum="1A838F9813D51B2E" modified="2022-05-25" version="1">MSGAKLQTKTVIPGISDADELSGENANGGQEIEKDNRSNKYDPREWEGVSSAAVDDEEADYKNLTFMGVPNSVQHRDDDEANEPESIHTIASNSTREAHNTSYNRRRHSKSPEAFQDVPVDAWRKEFKSALRRVSSLSRKPAQRLKRYSMQSQLMSQEPSIGNTKYHRGDRDLYTQKLASDLIDSLMAGCPAALFASTQFLRDEHGRRRAPLLLAMLGVEVEPLPLNNTMEPNDGAARADSPWGDEGETTAFENNRGRSNSNSSGSSTILSFEKPWGDHSKRLFKIHLEYGIGENRLKWSIVRNYKELSNLHGRLKLVSFQQNTVNKLYIDHNRYHKIHIPHFPHEDDYKEGFTDRIKSKQSARDSGPEVRRGKQGRAVSPDFSRLQGDISRASSIRSSLDFSIDRINVKHLQDLIDEKEDVSKPMHLRLERYLRLLNIALCLRPQANRLFQFYELSPIGNLLSYEDGPQGKQGYLIIRSTARAQGWRVSHFRFNDFKDMIERHTTKWFLVRHSYVMYTSDIYSTTPLDVFLIDHQFQIRCSGSNRNVLEDENIEQEWNQENSKKISTKLLITLENSERKLQMICKSEASLRQWISSIRQMAKSSPWAKKHRFNSFAPIRKNVFMKYLIDGRDYFWALSQALVMAEDVIYIHDWWLSPELYMRRPVNGHQQYRIDRILKERAENGVKIFIIVYRNVGSTVGTDSSWSKHSMLALHPNIHLIRSPNQWLQNTYFWAHHEKMTVIDNTVAFMGGIDLCYGRYDTPEHVLRDDHEKIPDQIFPGKDYSNARVCDFYDLDKPFESMYDRTAIPRMPWHDVHTMTIGEAARDMSRHFVQRWNYLLRQKRPSRPTPLLTPVSDFTEEQLKYSPFFQMLRPRATCEVQVIRSAGYWSLGLKETEHSIQNAYLKLIETSEHYIYIENQFFVTTSHWDGVVIENKVGDAIVDRVIKANSEGRTWKAFILIPLMPGFDSPIDQPEASSLRVIMQCQYQSISRGESSIFSRLRKLNIDPLQYVQFYSLRKWSTLGPHDKLVTEQLYVHAKVLIVDDRACIVGSANINERSQLGNRDSEVAAIIRDTDLVKTKMEDKEYYAGRFPWELRQRLMREHLGCDVDSVEIVERKFGRLKDLATKNYKTLHLLDSGATKAEKQTSAMIELAYREVFEVECSKAWQSKYGNQTKLAKEDEKGVHTPEGQKTDNARVDTLFNPPVDSRELGDQKSALPDSGLQTAYTEEEVNLHHATNYHSFNYRAGEENIGIRDKKPISTDPRVANNPKHSDDVCGLGPDEWRKVTKKFKEDVTQQLRTWAIDALGTVTIDGDKRSHSCFLPNKNDIESYLQEDNVLLEQKWDMLKRICYLQYLAHRKKKRESDDLNRKKRHAKLHKHKTSQILGQEELDESAIEELLAQLAPRFGGDRLSPNNLLKLNFIDPYSFEDPLSPSFVDDLWFSVAMRNTLLFRLVFHCQPDNAVQTWRDFKDYKRMTREFTQNQDKLSDEENQDENKPDQDKNGEHGIDQGAEGSIRRGQTLKDLHAKDLHGENVGEEMVRKAIAEPRNIEISKAVATKMRLAGSLLYGYDQRVFDKHTARRMLQRIHGHLVIFPTEWLAREVESKNWFYSADRLPPIEIYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T595</accession>
    <name>A0A8J2T595_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BN860_09912g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_09912g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T595"/>
    <dbReference type="OrthoDB" id="437889at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000063">
        <property type="entry name" value="Rho GTPase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF128">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN RGD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="5">
        <location>
            <begin position="24"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="461"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="322"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="387"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="326"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="441"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF88423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="655" mass="73841" checksum="B9F6BD1381E2EEF7" modified="2022-05-25" version="1">MSSAAKGSVATDDEPQDIQLLDKPEIQSVLNSDVAINALLGRLKQSLLTCEEFIKFLRKKVIFEDEHVEELGKQFRHFFMDTTTSSSSLKRKIHDILEYDGKLAKVKKSYVSALQKMYDEMSALLFTMTKMRKSVKENSRRLEKDVSDSIHAAEKSQSRYNSLCQDWEKLRMTDPTKTKLTLRGSKTTKEQEEELLRKIDNADLEYKQKVDHSTSLRSTFINKERPKIVSELKDLIMETDTAMSIQLQKYSIWTENLILNSGVTVAPIESTKSMRHVAASVTISKDLYSYLNKYNSSGKTSSMVNRNLIPVNYKKHASMIKISGPSPVTNTNVAPKSLSSSFSVDQGKSSKNRKPSSQQSNAPVPINLNGSSTSAKAAVVPTSPFVANKAPSAKDKKPSEQTPSDQNFKTLDPGNSNNVRIPSIATDLTMATSESDRPLSHVQTNMSMPPGTQKNFRTFGVPLEDLVEYEQDMVPAIVRQCIYVIEKYGLELEGIYRKTVDHNAVSKLKEEIDKEPDNVSMILPSKNYNEDDIYLVASLLKGFFAALPDTLLPEEITEEIKTCLSIPDEKTRKNYMHGLIYKLPDAQYWTLRALLFHLKQVVSHEPKNKMGAKQLYVVWGPTIVSPNDEDPDNVNFQIKTMEVLYDVADQAFEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5B2</accession>
    <name>A0A8J2T5B2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Polyadenylate-binding protein</fullName>
            <shortName evidence="14">PABP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">BN860_11782g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Binds the poly(A) tail of mRNA. Appears to be an important mediator of the multiple roles of the poly(A) tail in mRNA biogenesis, stability and translation. In the nucleus, involved in both mRNA cleavage and polyadenylation. Is also required for efficient mRNA export to the cytoplasm. Acts in concert with a poly(A)-specific nuclease (PAN) to affect poly(A) tail shortening, which may occur concomitantly with either nucleocytoplasmic mRNA transport or translational initiation. In the cytoplasm, stimulates translation initiation and regulates mRNA decay through translation termination-coupled poly(A) shortening, probably mediated by PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5B2"/>
    <dbReference type="OrthoDB" id="19742at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12378">
        <property type="entry name" value="RRM1_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12380">
        <property type="entry name" value="RRM3_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12381">
        <property type="entry name" value="RRM4_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000211">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000520">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000648">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006515">
        <property type="entry name" value="PABP_1234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034364">
        <property type="entry name" value="PABP_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01628">
        <property type="entry name" value="PABP-1234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 13 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM" evidence="17">
        <location>
            <begin position="40"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="17">
        <location>
            <begin position="128"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="17">
        <location>
            <begin position="221"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="17">
        <location>
            <begin position="324"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="18">
        <location>
            <begin position="497"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="293"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="11"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CDF88504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="584" mass="65279" checksum="AEBF176FF36B348D" modified="2022-05-25" version="1">MSDITDRTAEQLEQLNLHETPQTAPSSTDSESQKVETSTASLYVGELDPTVSEALLYDIFSPIGSVSSIRVCRDAITKTSLGYAYVNFNDHEAGRQAIEKLNYTPIKGVPCRIMWSQRDPSLRKKGSGNIFIKNLHPDIDNKALHDTFSVFGDILSCKIATDETGKSKGFGFVHFENDEIAKEAIDAINGMLLNGQEVYVAPHVSRKDRQSKLEEAKANFTNVYVKNINPETSQEDFEEFFKKMGPVTSIHLEKDQEGKLKGFGFVNYETHSDAAKAVDELNGTEFNGQQLHVGRAQKKHERLQELKKQYEQSKLEKLEKYQGVNLFIKNLDDSIDDERLREEFAPFGNITSVKVMTTENGTSKGFGFVCFSTPEEATKAITEKNQQIVAGKPLYVAIAQRKDVRRSQLAQQIQARNQMRYQQVTAAAAAAAAGMRGQFMPPMFYGVMPPRGVPFNGPNPQQLAAMGGMPKNGVPPQQFRNGPMYGVPPQGAPQQANFMRNGANANQFYQQKQRQLLGEQLYKKVFARTSDEEAAGKITGMILDLPSQEVVPLLESDDLFEQHFKEAFAAYETFKQEQEQHAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5I3</accession>
    <name>A0A8J2T5I3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peroxisomal ATPase PEX1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Peroxin-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_15676g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5I3"/>
    <dbReference type="OrthoDB" id="2187at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19526">
        <property type="entry name" value="RecA-like_PEX1_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000149">
        <property type="entry name" value="Nuclear valosin-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015342">
        <property type="entry name" value="PEX1-N_C-lobe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF12">
        <property type="entry name" value="PEROXISOMAL ATPASE PEX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09262">
        <property type="entry name" value="PEX-1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="458"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="738"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1031"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1039"/>
            <end position="1052"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF88669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1052" mass="117507" checksum="2BCDF785D61D0178" modified="2022-05-25" version="1">MASKPSTLHFTAIRIHFSNEIRGNFIRLPSSIVQTLESTGISIQEFGIGIFREGRELLHVGWDGYESQGYINGQPSIDINPILAKEFNLFSGLAVDLTISRYGDAQAASEVYVEPETSDDWEIIESNSMFFQDEILHQTRVVTVGEMLICYVDNVVSKFRIQKIIPENLKSAKINTGSLIIVAPRENKLRAPRDSPKHQELKKIPHNATTLKRTVIRGKEVELRNVEARVHPSDLESEYAFISIVHNSFDIKHNQSVKNTINKYSHNIAVTVASDVTIPNGSILLSPLVWNSLYTSPQNGLKLKVEFFDGHSIISDPSKIKILVKPLVDEEEATRKDRRKILHTPPSQGGPSQAVMDLISSLKCSIVTHKMICAQEKVIIELSLDSNGKHPLVADFKENPIDDNWQFLATEEVKLPLADLNSNLEINPFLKKAMGLEHLVATDLVEEMTGFLTLPITPSCGVMLEGGPGMGKTTLLQQVTNRLVLIHGKYVRYVDCDSLLESSNLGRMKQLLQECCSLCYWHAPSILLLDNASTLFPSVKSEDPQQAQLQRGAPTSTKLAMTLINCIQAISKKTSEAVRVVLCDHGRNSLDDSFFDKHFISETFELKALDTEARTNMVQFYLEMANSRLEDDLQSRDIALETEGYSPLDLKLLVEKMCYNLKIIQKDDTFTSLLNKNIFAETLASFTPSSLQGVKLTKGTGVKWDDIGALSAPKRLLLETLEWPTKYAPIFANCPLHLRSGILLYGYPGCGKTMLASAVAQQCGINFITVKGPEILDKYIGASEQNVRKLFERAQSVKPCILFFDEFDSIAPKRGHDSTGVTDRIVNQLLTQMDGVEGLDNVYVLVATSRPDLIDPALLRPGRIDKSVLCNIPNAHDRYDILRAITAKMKIAPGIDLHQIVKRTKGHTGADLQGLCYNAYLKAVHRGLNEQEEQQQQQRQKEEFGFKDKLESQEIVHSVINGGETTEILQCLRSLVEINANRHQESKAETNLPTPIITQQDLLTAAEETKPSISAHELNKLRSIYDRFQDNRDVDMPNGENSIETGTRTSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5I8</accession>
    <name>A0A8J2T5I8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleolar protein 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 6">Pumilio domain-containing protein NOP9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_09516g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP9 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5I8"/>
    <dbReference type="OrthoDB" id="392571at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040000">
        <property type="entry name" value="NOP9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22493">
        <property type="entry name" value="PUF_NOP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="627"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="637"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF88406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="655" mass="75960" checksum="BCE1EDCC8A1D5335" modified="2022-05-25" version="1">MGKPRGRKLQKKQQRDEFEPPLEVNHENEQLTNVPSSVSDPQMFFGVLDREELEYFKQAEATLSIDAFESTEEKQHFVTSVCDELKGKEYKLVTSQICSKLVERLILECGDDQLKSIFKAFNGVFYNLSCHKYASHVLETLFVRGAALVEKELLTPSFDGQQDDEPIISMENAFLYMLNEIKPHLRSMVSHQYASHVLRLLILILASKNLPKTTQNNSTLRSKKSKIARKMIDIKDNDDFDRVHQTPESFKLELKEFLKILYDNFTENTVSRTDISPTLVTKFRELCVDPVASPVIQLIIQVEGIFDRERSFWRLVFSTTDEKDPKEESFVEYLLSDPVGSHFLESVISFARTKYVERLYNLYMKDRITKLAQRDTTGAFVVRALLKHLKNKEVKQILSALIPQLSILLNSNIDFGTSIIEASIQQNNFLRDEVISQLVEKYYSPQSDSRNILESCLLLSSSTLGNTRDDWPTAEERRRSLFLEQLIDYDRKFAEITVESMLALPEERLLQMCYHGVFSHVVEHVLKPTLIDTIKRKLLLNVLCKDVVNMSCNAYGSHIVDKLWDFTAKLTLYKERIANALNKEAEKVKNSSYGRQVWKNWHMEQYLRRRGDWKTLIKEQGFAIFPDAEPTKPKVNGPQNKRSFRSNLPPTKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5K9</accession>
    <name>A0A8J2T5K9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">BN860_16380g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_16380g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5K9"/>
    <dbReference type="OrthoDB" id="427452at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000024">
        <property type="entry name" value="MIR1p Mitochondrial phosphate carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF12">
        <property type="entry name" value="MITOCHONDRIAL PHOSPHATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="19"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="118"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="216"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF88699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="315" mass="33168" checksum="8A3BAD1499CA3768" modified="2022-05-25" version="1">MSSPAVPKPAVVVPEYTLSDYAKFALAGAIGCGSTHSSMVPIDVVKTRIQLSPEVYNTGMVGSFKKIISEEGAGALLTGFGPTLLGYSIQGAFKFGGYEVFKKQFIDILGFDTAASYKNSVYMGSAAIAEFFADIALCPLEATRIRLVSRPDFTSGLVGGFSRMLREEGVGTFYNGFTPILFKQIPYNIAKFWVFERASAIYFGFAGDKDKLSQSAITGINLGSGLTAGLAAAIVSQPADTLLSKVNKTPKAPGQSTLNLLGQLAKQLGFFGSFAGLPTRLVMVGTLTSLQFGIYGSLKKTLGCLPAIDLGGGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5L3</accession>
    <name>A0A8J2T5L3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BN860_10022g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_10022g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4070686at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140445">
        <property type="term" value="C:chromosome, telomeric repeat region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14267">
        <property type="entry name" value="Rif1_CTD_C-II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022031">
        <property type="entry name" value="Rif1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22928">
        <property type="entry name" value="TELOMERE-ASSOCIATED PROTEIN RIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22928:SF3">
        <property type="entry name" value="TELOMERE-ASSOCIATED PROTEIN RIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12231">
        <property type="entry name" value="Rif1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Telomere-associated protein Rif1 N-terminal" evidence="8">
        <location>
            <begin position="258"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1267"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1321"/>
            <end position="1380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1464"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1499"/>
            <end position="1566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1277"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1327"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1355"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1371"/>
            <end position="1380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1473"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1500"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1547"/>
            <end position="1562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1623" mass="184304" checksum="D48B23A62EE92626" modified="2022-05-25" version="1">MSSPPGQSTEKRMRAIDRLDQHMIKRSKSNNRASSPVPFLSWITQKLPPPSSLLGQRDAPTQTSPSPKRRKTRSLSPSKIAEPRNSHVGTIPDENRYGTGANSSPIKKSVAFSDKLESSPTQRSPRSSPRPTSVSKPSKSILRKNADTVRKSANDVTVRRSELMLTSRIGSASLENNSGAVDPHSVDYWVGGEVHSMLEFNNVHEFKNIVEGGLKFLSQKDAVSITKRFEIYATFNNIMPVIPGKGSSEIAERKINVLIDHMDIVVDVCLSHLEEEQEKLLSTNSKKDPFVSRLYVQIVRFLNSILSNFKIIKWLTNKPQLQLKFKSIYRYSIEALTHNNTNKVIVAAQVAFLGDVKFGSFFLNDEELTSIIHTIPTIKEIQSTNLICEKLILIKNLLAKYPRLMIENVSLWLCGEVLPRILIDSEINCSKIVLTAVSTILDLLKKCLDVSKGHEDIYNSVQICTVREVVPAKLLPKLLPSQNDPENIISQTLGQLLRKQLKYLILVKQEYKLAMDLWLAMTGLLYNSTDRLQQLTSATDREWLDLNFLCFQAEDPQAKLLAIKVWRILTYSICSHLETFSTENLRIIKLLQRPFTFSREDEHDFNIMEGLLFHLTGIIYTAFSGTNNLSSTKFDLFWEHLVRPIYVERILISQSIQLKARANALLLRLLGGKIAEPQNKQVCKKIVHSIKIIASAGVTARDIQPLPLNVIKSSYEAIKVLVFQAIESDSSNLCQNFDLITALLKLLPNKFVDKEHLREFTNAIFEVAIQRKEDQNLAEMFIQITCCLATQFATLFFNEMKIFEEYLSKFGFILLANGETKIKLLKELIVGLRGKMSEFFIIEAFLQQGDLSTKEYVSNWIGSMLLSPTMPHEHFSSLVNIVTLIPTTEVLENFLNLSSKVTYDVDLFSLLNIQNWEVQGIIIFVKNSILRNHNAIKPELNLFLQSVLPNSTPIFSEMLPFLCGANHYNIVRYTLTKSPKFLNLTTSVDKKSFDEILPLEILPVFLQSLSDYQDTIQLRILQWSMEHNQINMLFGHYETLEHYLFKKEYNTEESSRRNQLLLDLLSSLLVGKNWLYMGKIIQSCMKNGQASPVTKTFSENDRESLFWLTPVTLASMINKCGSLNSSLIEVIKKCFSRKPVDFNIELTRELIDRNESQVFFLCGKEVVAFFTVRSRMFLLSEQDAISALFEMLMTSLMCQKDSLILDFVQELYANIPQECDSHLLKILIIAVKKLQAKGKHLKKYDRYHRISGELLSILSNLTITSENVGSKDDSARETSQNNESNFTEKISKISMDPKSNAVDSHDRLEIQVYATQTKATQIEEPVSLEKKSKNDEGRHISSDHSVAENRDLSEETTIQSNINYNKEGPEDTTSINTGKSASQNILEVQKSKVSKVESTDIAFVSSPSCQKTLFDRDSSTNVTKTNDAVLESTASLERGDDFAPVNVAPKDDFLEKMEEKIKIKNQESSEPIKGIEENHKDVSQMSQIRIPIFNSSKLQNKLDLEGPRSDKGKETLREARDTSVGKACDRDAKHSQGADLESSQEDNESHNVDNTSKEDYSKEPTPILRLHFPSKKARKLVSRLRGFTVEDVSKISPEEKRNLRIELLDFMMKLEHESLNGEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5N3</accession>
    <name>A0A8J2T5N3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_16292g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_16292g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5N3"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034593">
        <property type="term" value="F:phosphatidylinositol bisphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043812">
        <property type="term" value="F:phosphatidylinositol-4-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662:SF2">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3-PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="521"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="554"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="SAC" evidence="2">
        <location>
            <begin position="114"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF88696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="622" mass="70708" checksum="FB406E65E0162D28" modified="2022-05-25" version="1">MTGPLLFAKTSEGLLFRSTTPSKRDAVIHLSNQEQALRVEGLEEFPLEGNITKIPALLGFISLKLNKYAVIASKVREAGRINGQLIYQVTEHLTIPAKENARIDSDESEYLKLLKMQLDKATLYFSYTYDLTNSFQRNASINTPSWRTADTRFFWNHYLTEELQELSTRDASIAEFIQPVIYGYVKVVDRILRATPISVGLVTRRSRFRAGTRYFRRGIDEQGNVGNFNETEQLLVVQTNTNAVTHLFSFLQTRGSVPVYWAEINNLRYKPKLVVKEGSSLDAAAKHFAEQIKIYGDNYLVNLVNQTGHELPVKEAYESTVSALNDPHLHYVYFDFHHECRGMKWYRVKMLIEQLSSMGLKNSDFFHAIISQEGQVKKIINEQKSVVRTNCMDCLDRTNVVQSVLAHWVLQKELETAGVIPDDDAWENNISLLRSFQNLWADNADVVSCAYSGTGALKTDFTRTGKRTKLGAFNDFINSAARYYKNNLMDGPRQDSYDLFLGNFRPYMSSLKSPFSDKRPWSVQVIPIIFYAALTVLGATIVFPKGKFTSSKNLAFFFSASVIMFFAAAHLASDGIQYVNWPKLVDVGFLVTQQTHDKDKKFTGVTYVQNPRFVRPGASKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5U7</accession>
    <name>A0A8J2T5U7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BN860_14642g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_14642g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the INSIG family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5U7"/>
    <dbReference type="OrthoDB" id="205546at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025929">
        <property type="entry name" value="INSIG_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15301">
        <property type="entry name" value="INSULIN-INDUCED GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15301:SF3">
        <property type="entry name" value="PROTEIN NSG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07281">
        <property type="entry name" value="INSIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="40"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="264" mass="29286" checksum="FA60FF38CC815F05" modified="2022-05-25" version="1">MSGNRMATGLNGKEKSNESFSNLTRPQLFSLYDEDITKSADSEDYERIRSRGSRASVSRPNSTGSAYEPKPSKLGQMLRFLSSILVLSLAGIGYNELSKNLHDNHKLHPELASRPLLLGVQLSKFFTGNMLPDWAAYAVEGILFGSCIPLIDHIFSIETRPTSWVSVLKTVNAMLGVTFGIRKVQWSSSLQAAIAWGLLNIILWLLFDGTVSMFVSCSGLGLLTCVACYGDVTDKSQLLYFVDFYFLGLLLFSKLGRYLFSNHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5U8</accession>
    <name>A0A8J2T5U8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit epsilon</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit epsilon</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_13938g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2B gamma/epsilon subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5U8"/>
    <dbReference type="OrthoDB" id="424572at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04197">
        <property type="entry name" value="eIF-2B_epsilon_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05787">
        <property type="entry name" value="LbH_eIF2B_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11558">
        <property type="entry name" value="W2_eIF2B_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000022">
        <property type="entry name" value="Translation initiation factor eIF-2B epsilon subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000066">
        <property type="entry name" value="Translation initiation factor eIF-2B subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035543">
        <property type="entry name" value="eIF-2B_epsilon_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051956">
        <property type="entry name" value="eIF2B_epsilon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056764">
        <property type="entry name" value="LbH_EIF2B3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044123">
        <property type="entry name" value="W2_eIF2B_epsilon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45887">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45887:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25084">
        <property type="entry name" value="LbH_EIF2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02020">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00515">
        <property type="entry name" value="eIF5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="W2" evidence="10">
        <location>
            <begin position="534"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="510"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="697"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF87763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="718" mass="81353" checksum="329261B75A306FA2" modified="2022-05-25" version="1">MAGKKTKSHPKAEMGQDDRLQAIVLTDSFETRFMPLSYDKPRCLLPLANVPLIEYTLEFLAKGSVKEVYVVCSSHASQISSYIETSKWNFPWSPFKIFTIMSPEARSVGDAMRDLDNKGVITGDFLLVSGDAVTNLEFDKMWDFHKRVHAKDKDHIVTTCLSKATQYHKTRAFEPATFILDKSDNRCLYYQEIPLASSKEKSAVEIDPDLLEGVDEFVLRNDLIDCRIDICSPQVPAIFQENFDYQMLRSDFVKGVLSSDLLRKHVYAYITDEYAARVESWQSYDSISQDFIGRWCYPLVLDSNLMSDQTYSYESGHIYKEKDVVLAQSCKIGKCTAIGSRTKIGGGSVVENCVIGRNCHIGAGCTIRDSYIWDNTIIGNESVINHSIIADKAKLGNNVMLNDGCIIGFNVVIEDSKRLEKGVRVSGTPIKSMDDSALGLSDSEDINEEPQEYAEIAIEICGAKGSGYLYEDPLSEDDHEGPTNEKMISGLSHRLEEACLSDASISSVPKSKKKRTLSTNSVYTDREENPSELEEDEEDFEFEGVATVKRAIENNHDLDTALLELNTLRMSLNVSYHEVRKVTVIALLDRVYHFIATQTLGPKDATVKVFSQWGQLFRRQAFDHEEHVDLMNVIMEEVVEQNFEKPDLILFSAFTTLYDTGILEEEAIYDWWTHVSKDPKFDNVKRFVAKWVEWLQEADEESSSDESEGEEEDQEEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5V8</accession>
    <name>A0A8J2T5V8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit gamma</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_14378g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2B gamma/epsilon subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5V8"/>
    <dbReference type="OrthoDB" id="10250549at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002183">
        <property type="term" value="P:cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04652">
        <property type="entry name" value="LbH_eIF2B_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051960">
        <property type="entry name" value="eIF2B_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056764">
        <property type="entry name" value="LbH_EIF2B3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45989">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45989:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25084">
        <property type="entry name" value="LbH_EIF2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EIF2B subunit epsilon/gamma LbH" evidence="10">
        <location>
            <begin position="370"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="475"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="264"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="483"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="494"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF88615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="514" mass="58169" checksum="7A28279492F5465F" modified="2022-05-25" version="1">MELQAFILCGQGHNLWPFSQGNLEQHHVSANGMTKALLPIGNRSMLEYVLDWCDQADFKEINVVGNSDDIDAIQRGLQNYLDLRDEQFKLVDKVLSGSTHNHHLQDPKPIKFIKSKAISTGECLQKELLERITGDFVLLPCDFITDIPPQIFIDQYRNRDSDNIAMTFYYKNFLESTDKKQAGKQFFTIYASNENTDARPVLLDIYPMADVKKSKYLQVRTHLLWNYPNTTISTKLENSFVYFCSHEICQLLAERKPGNGTISKNHEEEGVQEESSKEHDDIMLDTTIKPNYFRQENQLVKDPINCHKSLGKIFRDLARRSWQHSKSRETVGMFILPEVGAFFRANSLHVYTEANRFILRIKAQTLATNTQTTTASASAIGADAVIGNNCTILEKSNIKLSAIGPGCKIGNRCRIAGSILLPNVTIEDEVILENVIIGPNGIIGKKSKLTNCCVEGYFNVEPKSNLKGETLTKLDFESDEDMSSNAAESSSDNESLEEEYEDEYEYEDDGLFDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5X6</accession>
    <name>A0A8J2T5X6_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Alpha-aminoadipate reductase</fullName>
            <ecNumber evidence="6">1.2.1.31</ecNumber>
            <ecNumber evidence="5">1.2.1.95</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">L-aminoadipate-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">BN860_09362g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(S)-2-amino-6-oxohexanoate + AMP + diphosphate + NADP(+) = L-2-aminoadipate + ATP + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46936"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="1.2.1.95"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(S)-2-amino-6-oxohexanoate + NAD(+) + H2O = L-2-aminoadipate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12308"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
            <dbReference type="EC" id="1.2.1.31"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(S)-2-amino-6-oxohexanoate + NADP(+) + H2O = L-2-aminoadipate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12304"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
            <dbReference type="EC" id="1.2.1.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pantetheine 4'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:47942"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.31" evidence="6"/>
    <dbReference type="EC" id="1.2.1.95" evidence="5"/>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00032"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004043">
        <property type="term" value="F:L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd17647">
        <property type="entry name" value="A_NRPS_alphaAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05235">
        <property type="entry name" value="SDR_e1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000046">
        <property type="entry name" value="L-2-aminoadipate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000787">
        <property type="entry name" value="L-2-aminoadipate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000035">
        <property type="entry name" value="L-2-aminoadipate reductase large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.30">
        <property type="entry name" value="Nonribosomal peptide synthetase, condensation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010071">
        <property type="entry name" value="AA_adenyl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014397">
        <property type="entry name" value="Lys2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010080">
        <property type="entry name" value="Thioester_reductase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01733">
        <property type="entry name" value="AA-adenyl-dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03443">
        <property type="entry name" value="alpha_am_amid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01746">
        <property type="entry name" value="Thioester-redct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44845">
        <property type="entry name" value="CARRIER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44845:SF1">
        <property type="entry name" value="L-2-AMINOADIPATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001617">
        <property type="entry name" value="Alpha-AR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01294">
        <property type="entry name" value="PKS_PP_betabranch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="18">
        <location>
            <begin position="837"/>
            <end position="914"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CDF89649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1374" mass="152594" checksum="F245690C148DC848" modified="2022-05-25" version="1">MQSWSEKLDNPTLSVLPHDYLRPFREPFTQEGSYSVAIPQLDTLSNFSDRYAVALSVWAALVFRLTGDDDIVLYVAGNKVLRFTIQPTWSFQELYKVVSTELTYLPATDSVDFDLLAEHIQKEQDLEKAPQLFRLAFLNGQPDFGLEHFNHHRIDFSLNLQVLHGSSVLEIKYNELLYSLERVAIIADQFAQFTAEVARDPSQCITKISLLTNSSESVIPDPRANLDWCGFVGCIQDIFQDNAEKFPERTCVVETPPFNSSKTRIFSYRDINRASNIVAHYLIETGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQNIYLGVAKPRGLIVIRAAGQLDSLVEDFIEKELDIITRIPSLALQDDGSLQGSLGNDDVLTPFEHLKDTRSGVVVGPDSNPTLSFTSGSEGIPKGVLGRHYSLAYYFNWMAKKFGLSEKDKFTMLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGKLAEWMGLHGCTVTHLTPAMGQLLTAEAVTPFPKLHHAFFVGDILTKRDCLRLQTLAENCCIINMYGTTETQRAVSYFEVKARSQDPDFLKRLKDVMPAGKGMLNVQVLIVNRNDRSQLCGIGEVGEIYVRAGGLAEGYRGLPELTKEKFVNNWLVEKGHWNSLDKDDGEPWRQFWLGPRDRLYRTGDLGRYLPDGNCECCGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPDDLSNFQSDVPADVASNNVVKGLVGYHLLAKDIKDFLKKRLASYAIPTVTIVLDQLPLNPNGKVDKPKLQFPSKKELDLAAANTVTDVDDSEFSPREREVRDLWLDVLPTKPTSVSPDDSFFDLGGHSILATRMIFSLKKQLDIDLPLGTIFKYPTIRQFAAAISNGGAKEAPPSSKSINYAQDADELIKTLPESYPSRKPFPKKASSASTVNVFVTGVTGFLGSYILADLLNRHPEHYNFKVFAQVRAQDEQSGLERLIKAAQTYGVWNPVFSERIEVVLGDLSKPHFGLNDAKWSQLANEVDVIIHNGALVHWVYPYEKLRDSNVISTINVMNLAASGKPKFFDFVSSTSTLDNEHYFSLSDKLVTEGKKGLPESDDLMGSATGLSGGYGQSKWAAEHIIRNAGERGLRGCIVRPGYVTGASKNGSCNTDDFLLRLLKGVVQLGKIPDIKNSTNMVPVDHVARVVVATALNPPEAKEFTVAQVTAHPRISLRDYLCQLGKYGYDVELVDYESWKKSLEVFVVEEGKDNALYPLLHMVLDNLPASTRAPELDDANAVKSLREDIAWTGEDVSGGKGATATQIGIYIAFLIKVGFLDSPKQHGELSLPTVTISERQLELVSSGAGARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5Y0</accession>
    <name>A0A8J2T5Y0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nucleoporin NUP188</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_06986g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Nup188 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="102511at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018864">
        <property type="entry name" value="Nucleoporin_Nup188_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044840">
        <property type="entry name" value="Nup188"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041634">
        <property type="entry name" value="Nup188_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048883">
        <property type="entry name" value="Nup188_N-subdom_III"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431">
        <property type="entry name" value="NUCLEOPORIN NUP188 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18378">
        <property type="entry name" value="Nup188_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10487">
        <property type="entry name" value="Nup188_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21093">
        <property type="entry name" value="Nup188_N-subdom_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup188 N-terminal" evidence="10">
        <location>
            <begin position="36"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin Nup188 N-terminal subdomain III" evidence="12">
        <location>
            <begin position="532"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="domain" description="Nuclear pore protein Nup188 C-terminal" evidence="11">
        <location>
            <begin position="1250"/>
            <end position="1599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF89550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1622" mass="183774" checksum="1222D33572B2CA6B" modified="2022-05-25" version="1">MAAQLGDDALSFVHVEYFINSYQLDSTDAVTSFSKISDFLDRCHDLILDVGKIDQVSNDHPRVSCLSVRGVKYENIKNESCEQASFLSKYLGLNYQESLRVITQMKVKTDDQIPLLSLATFVYQERNAILTTMLLLLNNSNLPVVQSSFAQLVANNVFTICVNLIGVLAKVITQYDKEVPEMPDGLTEREWEEFLQVKKAYNLIYMTNILKLLTHLMVNADLPTHLVEKWFTFLDETRSQLVFMTDVNSSIPGLISHKIESLIVVNTMLVLGLDSRSSAINLDSLYLKDANCFKRIHNILEQDPTNPVILYMWSFVLFTKSFLLEENPDLELSFIQQVFGKVPVSRETAVFAARAEKAGVFGAISRVSESLSSESFYPAIISSFLTFSLNFIPMNVQTSAMIKDVLLRTPQEFVENFLASDEFEKHLTILRAKLPLIDEALLPLVNTSAVHNQFANFEWNELHTYTTKFRLGELDYDIVDDVGESNMDLIILKKEALVKPYLKTNQNALISIPEDTKGKILPTPTKGDEDIIVFLYNYNGWSVLGYILQSICDAYIDNSEGLDDSTHYLLLAVLDLVANAVSKRSSFERAVEILQHLSTYTREKNITSVFFKIFEHSLHKRDYKLLCTCSKFVHSLFAHFPEIVWSHWARSVLLDRSGKTGMANVVLGSIELPIGNYEFTITLIKLTNEMVTEAVSLKADFPLKTKSEMLDKLVIHLIDVYESYQFWKYTNVIQRFELGFFLNSLFTKILYNVYGVDPASSPKVKVTNVLATSGSLILDSFLASQSPDVRAAKSLLNILLSPLNSQISLVGDRAFGHLYLRLMKHAYELAFLLISTRGSLKMPPSTLEKMIFSESSKFVDIYNSVPLLKRHVVKLFKSLVEVPWSDDYLFLLSYLGQRHSKLLLNSISFDLEGPLADHKLAKDIYMFFSALMESKQDGLSILFLTGNIASNKTEERNNLPNHRKSILSVLKKNALHLDALPESVGCCLLDAIAYAFNTWANAKDSKADSEFITALLNHLRNFRPVSTTSMQEMVAMSGKYKLISRVVEIFALYLFTSTDVDSQIFQLLNQQDLATLVNSFFEIDGYNKELHDNLHNKFEEKWPKLKLSSFALSPLFQPGASSNEMVFAIPLMDQFFGNDEKWIGSKDLLGYRDEVIEASLNLQYVSHQIAAAKAWGALLTTFVKKTTQSLKDTFIDLVCHFLKVNIERGIKSPLFTQVYCERLELSFYILYAFQKRAQSIPEKKLLMLLNQLITIFKSDEVNYLGNISYSRNRNFYRPVLRSILILLDAVSTGTHFVELISDELLEFFELSFCKGFHMILSEVLSDISTSTSNGKQVIVYNMAPRIQDLFLLLSLLTKIKALKPLDSFNVILASSLNEVGTVKVILSLYSTSHLLKVNDEALLGPLTLTFVSELCSIKSVAEKFITNGLFAVLLESPLSVAIQKGNIKPENKPSLHNIWTNGLLSIVLLLLSEFGVKVLPECCLFVSYFTKQVDTAIKRWSDSKLAVSSALIKETSQLVLLQKMLSALNYQAYLVNSKTHTAVDEKETYVELLPGLDTEQEKNTLNIALNRLLTHPKYLNSRIVPISLEEARLLEEDHTRAEFVKHISREIKKLQESLFRDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T615</accession>
    <name>A0A8J2T615_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">rRNA biogenesis protein RRP5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Ribosomal RNA-processing protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_00496g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the biogenesis of rRNA. Required for the formation of 18S and 5.8S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="412781at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05702">
        <property type="entry name" value="S1_Rrp5_repeat_hs11_sc8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05693">
        <property type="entry name" value="S1_Rrp5_repeat_hs1_sc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000671">
        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000155">
        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000159">
        <property type="entry name" value="rRNA biogenesis protein rrp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000196">
        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000394">
        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000423">
        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055430">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045209">
        <property type="entry name" value="Rrp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048058">
        <property type="entry name" value="Rrp5_S1_rpt_hs11_sc8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048059">
        <property type="entry name" value="Rrp5_S1_rpt_hs1_sc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 11 PRE-RRNA PROCESSING PROTEIN RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270:SF10">
        <property type="entry name" value="PROTEIN RRP5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23231">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="112"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="494"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="591"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="679"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="778"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="879"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="987"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="1060"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="1161"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="1249"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="157"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1328"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1396"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="71"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="163"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1328"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1341"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1358"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1400"/>
            <end position="1409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF88725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1709" mass="191631" checksum="D1E2867357F575A0" modified="2022-05-25" version="1">MPDKRRRDESPLVREDSTKQPASSLLKNTEVSFPRGGSSALTPLELKQVANEAANEVLFGGNGETTEIRKPAKKKKKTSRKRSGSIDIGTVGEGDNKIDFLEHINFKTLKPNSFLLGQVSAINKHDITVSFTDGISGYVTMTHISDQFTAHLEELDDKMDGSNDNDNESEDYSENEEEASKTKELPQLKHYFTLGQWLKCRITSNSALDAQKKKKQKKRIELSIEPHEVNDFTEEDLNKHSTVQCSVKSVEDHGLLLDLGIEGLTGFLLKKDIPQDWQCLPGSVFLASVMKKSGRTINVNFDFASKANKITHISSVDAVMPGQVIDFLCQKISPHGIVGKAFGMVVGFIGISQLRTFDQDQLTHQYAIGSNIKCRIIGSLLTKSGERALLLSTLPNIMCLDSRLLKDEALNAFPVGYVCEECKVLGRDRDYIYVALDEERLGHVHISRLGDVDVTKKVNARILGYNCVDRLYQLCTDPVMLSLKYLRSEDIPAGELVTGCEVLSVSTEGVNLKIFGGQFKAFVPPQHISDVRLVYPERKFKIGSKIKSRIQNVDSRGRIFATLKKSLVSLDEEKAKLITSFESAFDIKEANGLAVATVQRFHPKGCIISFFGGVRGFLPNAEISEVFVRRAQDHLRLGQTVIVKLSEADEKRKRIIATCKISNDQVAQQKEFIDGMVPGRTMVDVVVAEKTKDSLVVELKDLALRGVIYVGHLSDSRIEQNRALLKRIKIGSEMKGLVIDKDVRTHVFNLSLKESMINDAKENYLPLSYDDVKARDEKTPMHGYIKSISDKGLFVAFNGKFVGLVLPSYAVESRDVDISKTFYVNQSVTVFLLRTDDTHQRFLLTLKSPKNTPEKENTVTNAINPVDESMKELKDYTLGTIVKGKIKAVKRNQLNVVLADNLFGRVDVAEVFDTLQEIKDPMQPLANFKKGDILDVRIIGNHDIKSHKFLPVTHQVAKGTVLELSIKPSKLRGENYRAINLVDVTEGDELLGFINNYSGSVLWLTVSPCLKAKISALDLGDNCADLSESIETRFPLGTALKVRVTSIDKEHAYITVTGRSHLIKGIKDAKPEMQVPARVVKVTEKYVLLDLGNGVKGISFATDALNDFSIPLLEAYKGMQNQVLSATILSVEEDTSKIKLSLRSEDAITPTINSHEDLKQGEVVRALVKGVTDKGLFVYLSSKIEAFVPVSKLSDSFIKDWKKFYKPMQSVVGKIVKSDDNSRILLTLRESEVNGELNILKNYGDIKVGDVLNGHIKNVTDFGVFVKLDNTTNITGLAHRTEVADNVPQDLGSLFGIGDKVKAYVLKINPEKKQVSLSLKASHFTKNDEQVRKDANGDKITEEDEVMEDVDYNHNESESEPEEIEEEIDVKKPAVSSDGLSLSADFDWTASILDQTQADESSESDDEDFTNAKKSRRQKDKNKLVEDKTIEVNTRAPESVGDFERLIIGNPNSSIIWMNYMAFQLQLSEVEKAREIAERALKTISFREEAEKLNIWVAMLNLENTFGTDESLEEVFKRACQYMDAFTIHNKLLSIYQMSEKYDQASELFKVTAKKFGSEKLNIWVSWGDFLITQKEAQEARAILSKALQVLPKRNHIDVVKKFAQLEFGKGEPERGRSLFEGMIADVPKRIDLWNVYLDQEIKVGEKSKVEDLFERVIGRKLTRKQAKFFFNKWLQFEEAQNDDNAEEYVKAKATEYAEKHPKPTDSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T628</accession>
    <name>A0A8J2T628_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BN860_16402g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_16402g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T628"/>
    <dbReference type="OrthoDB" id="24581at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000014">
        <property type="entry name" value="Mitochondrial NADH kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000034">
        <property type="entry name" value="Mitochondrial NADH kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF26">
        <property type="entry name" value="NADH KINASE POS5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF87872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="422" mass="47074" checksum="B95D9F9A6E6D625C" modified="2022-05-25" version="1">MLLKLPLRFSRFYTSLEYPAIRIRDYSKYVTLKPVTDLRLGTSPDFVTSANSKLQSLIWQKPMQNVFITKKPWTETTRSSMVHFITYLHDSFPEINVIVQPDVAEEIAQDFRSNPRQDPHQPHVLYTGDAEDIVSRTDLLVTLGGDGTILRAVSIFGNKQVPPVLAFSLGTLGFLLPFDYQDHKKVFEQVISSRAKCLHRTRLECRIVRKGEKEPCSLATSLHAMNDLFLHRGNSPHLANLDIYIDGDFLTRTSADGVAFSTPTGSTAYSLSAGGSIVSPLVPSILLTPICPRSLSFRPLILPHSSHIRVRVGSKATQGPANAMVKLSVDGIPQEDLRIGDEVHVVNEVGTIYLNGSQLSSSSNQREVAERRKTIKNSGIYCIAKTENDWTRGLNELLGFNSSFRLTKAQKFKEDLENNMTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T660</accession>
    <name>A0A8J2T660_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit C</fullName>
            <shortName evidence="4">eIF3c</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 93 kDa subunit homolog</fullName>
            <shortName evidence="4">eIF3 p93</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Translation initiation factor eIF3, p93 subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">NIP1</name>
        <name evidence="7" type="ORF">BN860_01200g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit C family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T660"/>
    <dbReference type="OrthoDB" id="29647at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03002">
        <property type="entry name" value="eIF3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027516">
        <property type="entry name" value="EIF3C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008905">
        <property type="entry name" value="EIF3C_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13937">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITATION FACTOR 3, SUBUNIT 8 EIF3S8 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13937:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05470">
        <property type="entry name" value="eIF-3c_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="6">
        <location>
            <begin position="610"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF88754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="814" mass="92490" checksum="EA4230744FFC7625" modified="2022-05-25" version="1">MSRFFAANYDYESGSSSSEEDLLSSSEDELLTSSGGEGEESDDSLFQEDSESESDVDSDDSDGKPYGPDWFKKPEFRKGGPSGGNKFLKGASYSDSDESDDEGKKVVKSAKEKLLDDMQTVYNKVESAEMTNDWVTILSEFDIISRLLIRAQQQNYGTPNIFVKVLAQFEDAVNATSQQEIKSKAASRAYNTTKQRVKKLARENEQLLEKFRENPEAFDKEPSAELDTSITEGSVPLFGKKSINLSSVASASSEAGFFSALRIVIDSRGKKNVDYSALIKTLEELMEIAKTPYETIMAYLNLVPIRFDVSANLSYQPVDQWKASYQDIMKLLSILEENIEKYQLSELAPKNDFIEDEPEDDEHGVKHILGSIFSFVERLDDEFNKSLLNTDPHSSDYLVRLRDEQSVYNLILRTQLYLEATLPEECRESHLARPFVKRLDHIYYKSTKLISIMESNAWKSVPSTSSSNFIPYKGDADEQYVTELVQTLAESLRQQKSGGLRKRAILYQVYFTAFNNNFGAAKEMLINSKIQSHINNSDPSLQILFNRVVVQLGLAAFKLCLIEDCHQVLNELLASSHLREILGQQTLQRISANVGSSSTADEREQLCLPYHKHINLDLIDLVFMTCSLLIEVPQMTAFYSGIKVKRVPYSQKSIRRALEHYDKSSFQGPPESLRDHVLHAAKSMQKGNWAKSIEFLRSVPTWSLLPNADYVLDNLAERVQVESLKTYFFTYKRFYSNISIERFSELFNLPEARVLEVLESVINEYDINAKLNEEKTFVTVEKGEEITKLEEVALKLNKEVKIAKERLNPSSARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T676</accession>
    <name>A0A8J2T676_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ZYBA0S03-11210g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_11210g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for box C/D snoRNAs accumulation involved in snoRNA processing, snoRNA transport to the nucleolus and ribosome biogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the BCD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T676"/>
    <dbReference type="OrthoDB" id="272357at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070761">
        <property type="term" value="C:pre-snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048254">
        <property type="term" value="P:snoRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23023">
        <property type="entry name" value="zf-HIT_BCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051639">
        <property type="entry name" value="BCD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057721">
        <property type="entry name" value="BCD1_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13483:SF3">
        <property type="entry name" value="BOX C_D SNORNA PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13483">
        <property type="entry name" value="BOX C_D SNORNA PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25790">
        <property type="entry name" value="BCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51083">
        <property type="entry name" value="ZF_HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HIT-type" evidence="9">
        <location>
            <begin position="5"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="287"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="297"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF89187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="351" mass="40386" checksum="55A4856A97277D2B" modified="2022-05-25" version="1">MESFCEVCHEVQFKYKCPRCFKKTCSLPCSQKHKTQDACSGIAHDPTTYIASEALKEADDEKHESNLLVQRDYQFLNKLKRSLEVDMRDGRLINKKVLQPYGSYAKRQHYDQECQRVIRRGVNCILLPKGMQRSLMNKSKWDKPLDLFVWSIEWVLCPKKKKDKTTEEPFKHVSHRIKETDSLLEGMGKIICEKCYEFYNLAEEGEPLPETKAERTQRLIDSGLKFYTKWFPYNVVRIMDSKKLIELDARHKSIAELFKNRTVIEFPTIFVAEYDEELPEAFTVVADDSTHSQRTANSNDSSNESQNNSDSDENSEPIEESSKPLTSSTAAESDGDSDGYDPGVTLDFLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6D6</accession>
    <name>A0A8J2T6D6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S03-09384g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_09384g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCP11 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6D6"/>
    <dbReference type="OrthoDB" id="276323at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010737">
        <property type="term" value="P:protein kinase A signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008862">
        <property type="entry name" value="Tcp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832:SF11">
        <property type="entry name" value="LD23868P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832">
        <property type="entry name" value="TESTIS-SPECIFIC PROTEIN PBS13 T-COMPLEX 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05794">
        <property type="entry name" value="Tcp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="124"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="278"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="22"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="66"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="278"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="961" mass="106280" checksum="085E903F6E9E52C3" modified="2022-05-25" version="1">MEQARPHRSTVTSAASPSQPQTTVTGSPSPTTTTTTTTATTTSAASTVAATSTTTEQKSHGGNNGEEMNTNKEGSNAPNNANQHKRLSTCSQEHAPEAGAQGTLPPTGSLLVSKYTEFVSSIGNHFTSNSGSPRHDEVHDQDDDNSNDNDDDQDQDQVQDENLDSNTGSNCLEGNSLFKQFDHKTGQFLRREPQESDAHNGVKRLFANKFRSHSVPAISHSSLKRLTTYCQNHHHYHYHQHAASGAVQKKRASRLHHHHHRHHHNHCFVQATPVAALGNSNTSTGSSGSSRSSSRKSGSSSSSSSSSSSGGSSSNGSSGIRHATGGNDTGVTSFGDESKHSTAAQASTDALGDTSTGRTTAINRNKNLMAGNALRNAILLCHQTQPSPTKYEELTLQERINAIRKQPGPLPLPPINLQCLREIDLQEIVKNPQLRHDIIFDPLLQFRPNLDGERGIKKKQLADKYWNDVKNEVFVYSQSPQVFQYNHTRLVPLFDTLREVLLTIVPQRETHVIHNILDTELNVQELLRGSLIMTNLSEWLAQLFKHHCAPMRDTWVDKMSAKFKEAEQESSLSKLIEGLRLVFQILEAMKLDIANHQIRILRPALLSNSVEFEKQYFQSLMSSDRVDLKSSLVWFSRKFEESVSQGRLQRGQAAPRDIYKLCIQSVISLLSCRKMVREYPTSLSFDHARLILLRADIRQIVCLLICRLLFKQLVANDTLLDKGAKSYIDSAGTLKRLKGEIVSIITDEHGNCRWTKNTMAIAIHLCKTITDLRDEYWARRGDTVESCGSSSSNANTAAPYSTPTLAQAKVDFAKSWLSKQTQPLSEVYGVLEDRVFRSLEELIFARSECTVDGRVKQDFVHLCSQSTTESINNLLTPPRDNSGTSVMAPSVATSSMTAAVPAPVSPAQAQTATAKATMQGLDMEEFDNVFRHLYAVINFHWSVFGSHYMDALGEQKGARVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6M5</accession>
    <name>A0A8J2T6M5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">transketolase</fullName>
            <ecNumber evidence="4">2.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_09428g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = aldehydo-D-ribose 5-phosphate + D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:10508"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 1 Mg(2+) ion per subunit. Can also utilize other divalent metal cations, such as Ca(2+), Mn(2+) and Co(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="12">Binds 1 thiamine pyrophosphate per subunit. During the reaction, the substrate forms a covalent intermediate with the cofactor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transketolase family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.1" evidence="4"/>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6M5"/>
    <dbReference type="OrthoDB" id="10267175at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004802">
        <property type="term" value="F:transketolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07033">
        <property type="entry name" value="TPP_PYR_DXS_TK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000003">
        <property type="entry name" value="Transketolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000003">
        <property type="entry name" value="Transketolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000004">
        <property type="entry name" value="Transketolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055152">
        <property type="entry name" value="Transketolase-like_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005478">
        <property type="entry name" value="Transketolase_bac-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020826">
        <property type="entry name" value="Transketolase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033247">
        <property type="entry name" value="Transketolase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00232">
        <property type="entry name" value="tktlase_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43522">
        <property type="entry name" value="TRANSKETOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43522:SF2">
        <property type="entry name" value="TRANSKETOLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22613">
        <property type="entry name" value="Transketolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00802">
        <property type="entry name" value="TRANSKETOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="15">
        <location>
            <begin position="358"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="420"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="118"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="471"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="479"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="483"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="530"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="14">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="14">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605478-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605478-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605478-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR605478-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR605478-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF89652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="682" mass="74235" checksum="3448537EA7ECD8B2" modified="2022-05-25" version="1">MPTHQYTGIDDLAIRTLKLLSVDQPSAANSGHPGAPLALAPAAHTLLKSIKINPKNPAWINRDRFVLSNGHACALLYSMLHLVGYDYTIDDLKHFRQLNSKCPGHPESHLPGIDVTTGPLGQGVADAVGLAIAQANFAATYNKPGYTLSDSHTFVFAGDGCMEEGISSEASSLAGHLGLGNLIMIYDNNSISIDGPTKLSYTDDILKRYESYGWEVIDLNDNADDPNALLYAIHQGMKSTKKPTFIRLTTVIGVGSLLAGTAAVHGSPLKPDDLKQLKKHLGFDPEQSFVVPQEVYDFYRKTVTQPGIEANEKWDKLYAEYQKKFPEEGKEVARRLNYELPSNWDSYLPKYTPADKALATRKLSEGVLEHIHAALPELMGGSADLTPSNLTRWQGAVDFEEPSSGLGNYKGRYIRYGIREHAMGAIMNGIAAFGANYKPYGGTFLNFVSYAAGSVRLAALSGHPVIWVATHDSIGLGEDGPTHQPIETLAHFRAMPNIDVWRPADGNEVSAAYKVAIDHRHTPSILALSRQNLPQLEGSCPIKAQKGAYVLQDVSKPDIILIGTGSEVSLVVAAAKVLADKGIKVRVVSLPSTDAFDRQSLDYKLSVLPDGVPIMSVEVLSTFGWQRYAHQAFGINRFGVSAKQSDAYKFFDFVPEGVSSRAEKTIAFYKGKEVLSPVHDAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6Q6</accession>
    <name>A0A8J2T6Q6_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">GPI mannosyltransferase 2</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_09252g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the PIGV family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6Q6"/>
    <dbReference type="OrthoDB" id="10252502at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031501">
        <property type="term" value="C:mannosyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004376">
        <property type="term" value="F:GPI mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007315">
        <property type="entry name" value="PIG-V/Gpi18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12468">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12468:SF2">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04188">
        <property type="entry name" value="Mannosyl_trans2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 12" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="149"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="198"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="343"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="403"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF89105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="425" mass="49395" checksum="BDC7434D935FB717" modified="2022-05-25" version="1">MFLQISASFTFCKLIQYGLVILTPTSQFDTSTELLLNRYTTESENFWNRVLWNKLLSWDAVYFIKAMVAEDGKPQFEHEYAFSLVWVQLVRFCAPSRELYSVLKVGVLLENVLHYLATFVLYFLTVKTFSSSTQKSAYAESLARKTSVLFIFTSAAGFLTGIYSEPLSFTLAFLGMWARQSAVFVALPKHVDTFYSRLPLYLVSSLCFSLATLNRPNCMVLGIYYVFDLWQMMKSRNYTKALLFPLLSGILLFLACIYQQYVLPYSTFCPQRGVWCEQLVIEPLTKQSFYGFLQSHYWNVGFLKYWTPNNVPNFLFALPNLITLPYSTIYFSRIYPYYTLRPLIWITASLVIIVVFFAHVQIINRISSFIPLHLWYVADRLVKKSNSKKKASLRGDDRIAHYYILWVIFWIPIQTALFASFLPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T735</accession>
    <name>A0A8J2T735_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Aspartate-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="4">1.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_11980g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the NADPH-dependent formation of L-aspartate 4-semialdehyde (L-ASA) by the reductive dephosphorylation of 4-phospho-L-aspartate. Mediates the second step in the biosynthesis of amino acids that derive from aspartate (the aspartate family of amino acids), including methioinine and threonine, the latter of which is a precursor to isoleucine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-aspartate 4-semialdehyde + phosphate + NADP(+) = 4-phospho-L-aspartate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.2.1.11"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:24286"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.11" evidence="4"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T735"/>
    <dbReference type="OrthoDB" id="1894490at2759"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00016"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00463"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00464"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18130">
        <property type="entry name" value="ASADH_C_arch_fung_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02315">
        <property type="entry name" value="ScASADH_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000200">
        <property type="entry name" value="Aspartate-semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000016">
        <property type="entry name" value="Probable aspartate-semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051823">
        <property type="entry name" value="ASADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000319">
        <property type="entry name" value="Asp-semialdehyde_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005676">
        <property type="entry name" value="Asp_semi-ald_DH_pep-lack"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00978">
        <property type="entry name" value="asd_EA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006416">
        <property type="entry name" value="PRK08664.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718:SF1">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000148">
        <property type="entry name" value="ASA_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01103">
        <property type="entry name" value="ASD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Semialdehyde dehydrogenase NAD-binding" evidence="14">
        <location>
            <begin position="6"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="13">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000148-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF89219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="365" mass="39335" checksum="9BC5373F561AB47B" modified="2022-05-25" version="1">MVQKKIAGVLGATGSVGQRFILLLANHPHFDLKVLGASQRSAGKKYVDAVNWKQTDLLPESAQDLVVRECKASDFKECDIVFSGLDSDYAGAIEKEFAEAGLAVVSNAKNYRRDPGVPLVVPIVNPEHLQVVAHKLETAKAEGKPKPGFIICISNCSTAGLVAPLKPLVEKFGPIDTLTTTTLQAISGAGFSPGVPGIDVLDNIIPFISGEEDKMEWETKKILGHLAADKSHVELLSPEEMKVSAQCNRVAVSDGHTECIALRFKNRPAPSVDAVKACLRDYVCDASKMGCQSAPKQTIHVLEQEDRPQPRLDRNRDAGYGVSVGRIREDPVLDFKMVVLSHNTIIGAAGSGVLIAEILRAQNMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T767</accession>
    <name>A0A8J2T767_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_10044g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T767"/>
    <dbReference type="OrthoDB" id="539158at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001035">
        <property type="entry name" value="Serine/threonine-protein kinase CHK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF32">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="15"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF88429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="514" mass="58619" checksum="B5AA9C11BAF5A3D0" modified="2022-05-25" version="1">MDQIDYCPLPVIKDVILGDTIGHGTFACVKSASLRVDPSVIIAVKFVHVPTCQKYGLSDKDVVGEVILQSKCSKHMNVLRIIDCNISRDYLWIAMEMAEGGDLFDKIEPDVGVDSEVAQFYFQQLVRAISYLHEECGVAHRDIKPENILLDKNGNLKLADFGLASRFKRQDSSRRVYMDQRGSPQYMAPEILHQEYYADTTDIWSIGVLLFVILTGEIPWDLPVMEDTNFECFMKNNGNLNVGPWARIDFTHLNLLRKILQPDPSKRVSLQRIRKHPWFTSKVSFCKNDGLCGDPVALARKLFSKLKVSLSDNDYLRFTQDHSGRSSTQPVHTDITAIEHDSLKNNSMAMTQGPLMRNEHGSDIMLTQEVDWAHHIRNDVAALQFCNDNLSRHGNFYFNPMKLTKFYTLEEMELVLPTLENALQFSGINARPNLHENFLSLCQKLGHEKAFPLHMNIRAMDRRGANLYGFASIVLIEGNLKSISFEKKSGDPLEWRRLFKKIALLCRDLVLIPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T783</accession>
    <name>A0A8J2T783_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ZYBA0S04-07514g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_07514g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T783"/>
    <dbReference type="OrthoDB" id="1924787at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15485">
        <property type="entry name" value="ZIP_Cat8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000007">
        <property type="entry name" value="C6 transcription factor FacB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF12">
        <property type="entry name" value="REGULATORY PROTEIN CAT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="10">
        <location>
            <begin position="96"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="724"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="886"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1111"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="893"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="908"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1126"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1162"/>
            <end position="1175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF89573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1191" mass="132807" checksum="194C1D86622CC7FA" modified="2022-05-25" version="1">MKDDLEARESSTGAKNAPNSMQAPRFIRTLGSQSLGGLRGNNSQRSSVSPPYEHGDDSKESSAVPQALLMNSSGPNSHRDSTCSPAGPRNYRVAQACDRCRSKKTRCDGKRPQCSQCAAVGFECKISDRLSRRAFPRGYTETLEERVRELEAENRRLVALCDIKEQQIHLFSQQHSPGGRRKDDERMLRELQSANGGSLNVSSTNLYLLNASPHTTQQQHLKKQPHVCDGVCCEGKLHVKPVSTNLNDPTSVSFEQSEAPGLPAVQALTSVATREQSNQLATLVALSVPRSTEEILFIPQLLARIRQIYGFTSKQCLYTVSLLSSLKSSLPEPQLMDYEPLERLEAVNLWEMDDLEQFFGRMLKFQFGAQHGEEPGLSIGEVEELVSLFFEQWSVHIPILVKEGFYRYFGQFKENVLHSLQLLKTPLQGPALAARRDKIISYKIFGCLLLMLCQMGLLTKTKSDRLVASHKYHRLASYYHRAVSLAYMNPYFGVFSTSLQSLQFLSLVLFYFLNVGNISAIYDLRGRVVSMAQQLRLHRCPSAVLGGAGSTMDKREQGDRRVLFWGIYYLDVFSALQLGVPRLIKDFEIECALPVSDNDDRTVNLAGQQIRLEGRVTQFSLAIIRFAKVLGNILDSIFKRGMTESITKQVSLIHENALDNWRRGLPQELMFELDVNGTINMDEFNKLKQMNATVEHSENIVLLVMYFLAKCMIHLPVVATRPLPTNEPGESNDTTAAASAAASSGHTEARESSSEVSQAERSSSSYVLLQQATNTLLNVLESLKTIYLPMPLNISRTKARFALLSARGSLEYIKGGSLFLDNKALLLNVIQDLEEDRKLELPGVISWNSLKLLDMSINLLLQPPNTEVEKLDKLLKKKLNHYNRLMGRPIVKSEQSSRAANLTPASSKGDEDTPPAKRVKIEEPVMPPPLNHEQASQEQHESHPQQPVSTQTAFAEALQLDPVLNGNSFGGHGLNPTLYDMPQHHSNHPQMQEPQLNAQSSDKLHGLFKVPSTADLLMDDPANSQLNMLLNTGELGGPDPIQPSTFTNLNSGFSGLNLSNLFGLEGSAAQGGATMGSGQRQQPDTGFNFAVDASLGLAPLLAWTPDRNSIVLDPMNEGEDLHSDQHQTALPPHQQQLHQQQQQQQQSEEQQPQESEEDLSMRKRSHRSRGRGRPRKHEENLHDLFCWQNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7F7</accession>
    <name>A0A8J2T7F7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine-protein kinase MEC1</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ATR homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">DNA-damage checkpoint kinase MEC1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Mitosis entry checkpoint protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">BN860_10066g</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PI3/PI4-kinase family. ATM subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00892">
        <property type="entry name" value="PIKKc_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000033">
        <property type="entry name" value="Serine/threonine-protein kinase MEC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056802">
        <property type="entry name" value="ATR-like_M-HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057564">
        <property type="entry name" value="HEAT_ATR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058681">
        <property type="entry name" value="HEAT_MEC1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012993">
        <property type="entry name" value="UME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF125">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE MEC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23593">
        <property type="entry name" value="HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25385">
        <property type="entry name" value="HEAT_MEC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25030">
        <property type="entry name" value="M-HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08064">
        <property type="entry name" value="UME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00802">
        <property type="entry name" value="UME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="21">
        <location>
            <begin position="1318"/>
            <end position="1863"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="20">
        <location>
            <begin position="1968"/>
            <end position="2271"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="22">
        <location>
            <begin position="2255"/>
            <end position="2287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CDF89680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2287" mass="264704" checksum="1C8E803FDA33B675" modified="2022-05-25" version="1">MDPHVKYLDELILAIKESRLSGGNDPKVSKILATLIRNLKDPRRNDDMFAKSMEALDLLLRSQPPREIETIFAEFVDVCVAHYASLQRVWLVKRKLGWWCKISVQLYGENTKLSLSKHIRTLLESCEQGLAKVLTGKCEVEEFYDHLKRLWVICYWFAGKHEFGNSITLLDSTLGFRSWDLQLQRLMRFVIYIFQSISLRSADYDRLRARYLSMVVENLTNECIATDQLKFTLFFLRQFMRLNFETADAAIPMAKAMLRIYLSCGDELIGTFVDHFPIERWLQTDLPVGTPIRKALLTIKYSLQKKGKIGDEPVEVCTFTESIQLENLRKLICSDVSWPPPLQDYVKSIDPGIVIDSENVIPSLLSHISKSIQVCFSTNNISKLICWTKVLARLACLESQQKQHTMDNWTVCKICDSNEPNNIFQTIDPDRPNCITESPSFQIMNQFFLLNSKLEDFSESLLAGILLCLQRIFVHYQPPKLPGGTYFDIVEKCFRNPNRYLRILSSRLIPLWNITNLHNSEDQHTAILIQFLQTDVKPYMTETSVLAWAQLTLTTSGDIFDTLLLKLIDTFNSSDYATHIMMAFQIKNIAHILKKTPYQLLSPILPILLRQLGKNLIERKVSFQRLINLLGYSAKTILQIYQRYIIPYAITQYKTDVFGEIAKITCNGETTEEVNEQKAILLDKNSRQIFAVALVKHGLFSLDTLETLFLNRLPSFDKRYVTAYLPDYKTLAEILKLYKNENDDNSSIPENRNMILCSLRFLVTNFERDKRHGSKYKNVTDWTPEQERLFQKHLQDNILGIFQVFSSDIHDIEGRTTYYEKLRVINGIAFLIKHTFKESIISALAQISICLQTCLEIEQVRFNALKCWQLLVVNLNDEELSTVIDGLVCYVLQNWSNFNNRLRLVAYDILDILIKDKTSLVLTIKPYTTLSLVTKTELGILSRNSQFARNVNKVLSNTDWMLIFSSNLQSTNKYVIYQNLDDLENFLKRKQSERSLDIFSKQGKDECEGIVRVLGALLDTSHKFKSTDDHLCQKCAKCISIIGVLDVTKHGLRRGRPIFNEVYDLSDDTQTIKFLIWVINDILVPAFWQSENPSKQLFVALVMQESLKYCGLSSASWDVNKKDLYPNEAKLWDKFNTISKTTLYPLLSSLYLAQSWKEYLPLKYPSYNFREGYISWIKSFCLDLLKTGTNEDHPLHVFSSLIREDDGSLSDFLLPYISLDIIIKAEPGKPFVELMNNIIMEFKCILEYKLDDLNHLQIDSLKMCYQSIFRVLEYCKKWVTHFKQSYNKANGTFIIKETRYNNMLKRVDQFVAKFPSDLLAQRSLETNSFERSALYLEQCCRQTKGENSQDLSILESLHRTYEEIGDIDSIDGLLKTFSTGNLMSKIEELQYSDNWKMAQNCFTTLGTFSFESPCTTKMLKSMYDHQLYSQILTQLEILTPKNLRYLKPEIKEWYRIGMEAANLEGNTNYLIKWISRIESLQEVSDPEMLLQYNMGKAFFYEYMRTPEKTSEYINKCFKLIGTHFISPSIATTLLKKQTLLMKLHGLYDVLLLSRTKSEWEYKNSADILGFRMKRVGADFEPNHYLLSVRKSYDLMKNDEYTKQDLADTFLNVAKLCRNNSRLDIASGSLMHCLKYEHPQAELEFAEILWKQGENDRALKLVKEIHGKYKDNPSVKPRDRAIVLLKYTEWLDLSNNSASEQIIKQYQSIFELDPKWDKPYYSIGLYYSRLLERKKAEGYVTNGRLEYKSISYFLLAFEKNTTKVRETLPKVVTFWLDIAAASMNEPGTSRREVLQRATEDICQHVEDALKKCPTYIWYSVLTQLLSRLLHVHPLSSQLIMQILVNLTVEYPSHILWYITVLVNSNSSSRVSRGKQILENYKKSPNTSLELVSEAFDLTSSLTRVCLKDAKNTSSRSGKSLERDFKFNMHMAPSKMAVPVRINLEMISPLSSDSMKSYRPFHSVVSIARFGSAYKVFSSLKKPKQLTIVGSDGNIYGIMCKKEDVRQDNQYMQFATTMDFLLSKDLESTKRNLGITTYAVLSLREDCGLLEIVPNVVTLRSIFVTKYESLKIKYSLKSLYDKWQHLSEEGRIKFYEDQLATFPPILYQWFLETFPDPINWYNARNTFARSYAVMAMVGHILGLGDRHCENILLDLESGKVLHVDFDCLFEKGRRLPVPEIVPFRLTQNLHDALGIVGTEGTFKKCSEVTVSLMRTNEVSLVNVIETIMYDRNMDNSIQKALKVLRNKIRGIDPRDGLVLSVPGQVETLIQESVSPENLGKMYIGWLSFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7L9</accession>
    <name>A0A8J2T7L9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">ER membrane protein complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_16072g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The EMC seems to be required for efficient folding of proteins in the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7L9"/>
    <dbReference type="OrthoDB" id="6745403at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008568">
        <property type="entry name" value="EMC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116:SF5">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010045">
        <property type="entry name" value="DUF850_TM_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035314610" description="ER membrane protein complex subunit 3" evidence="8">
        <location>
            <begin position="19"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR010045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="264" mass="29130" checksum="EDFADEBE25E26AFF" modified="2022-05-25" version="1">MALSLLSMIATGSAGAVAELTLDPKLKYWVLLPISIVMVLTGILRQYIMDLIAPSPKGVPRVKLTESQWIYKAQALLGNGSQLSEEAFQAKKEFLTETLADAKYLVKMNTEGDVANPLSDPNFSESMFSMAKGNLANFIPQTIIMWWVNHFFAGFVLMKLPFPLTPRFKEMLQSGVMTADLDVRWVSSISWYFISVLGLNPVYNLILSTNANGDFGVQQPPVMGGAPGEPQPEAVMKSLANDLTIAQHESCFDSIEERVLAKYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7N4</accession>
    <name>A0A8J2T7N4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="19">ZYBA0S04-09340g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">BN860_09340g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7N4"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000109">
        <property type="term" value="C:nucleotide-excision repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16567">
        <property type="entry name" value="RING-HC_RAD16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000753">
        <property type="entry name" value="DNA repair protein RAD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001864">
        <property type="entry name" value="DNA repair protein RAD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF12">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="181"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="520"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="607"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="107"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CDF89648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="774" mass="89519" checksum="D60C25C5819C9E13" modified="2022-05-25" version="1">MVEEGGFIRRSRRRNAKRMSYAESDEDVKQESDTEYQPDAGVKKESDEDVEIIEDPEIKKEEDPGEIPGPESKEEQDVDDDDDDDDKPLSQRRKRSRGSGSKEGQQKRGKRQTKKKKEPKPKISNFDRNTEKLFQQHPQLRSVFPELQKAVIPAPERAPQPAGMTIRLLPFQLEGLYWLEKQENSKYNGGVLSDEMGMGKTIQMIALIMSNLAHSPTLVVAPTVALMQWKNEIDEHTGGALKVYVFHGANRTNNVADLKGYQVVLTTYSVLESVYRLQAYGFRRKTGLKKERSVLHNVDFYRVVLDEAHNIKDRQSSTAKAVNELRAQKRWCLTGTPLQNRIGEMYSLIRFLDVEPFSGYYCIKCDCNSKRWRFSDNMHCDHCGHVMMQHRSFFNHFMLKNIQQFGAEGPGYEAFQRIQVLLKSIMLRRTKVERADDLGLPPRIVTVRRDYFNEEEKDLYRSLYTDVKRKFNSYVEEGVVLNNYANIFTLITRMRQLADHPDLVLKRLPGSDASDGVIVCQLCNDEAEEPIESKCHHRFCRLCIKEYVESFMEESQKRLTCPVCHIGLSIDLSQQSIEVDMDSFHKQSIVSRLNMQGTWRSSTKIEALVEELYNLRSDNRTIKSIVFSQFTSMLDLIDWRLKRAGFETVKLQGSMSPTQRDETIKYFMKNIRCEVFLVSLKAGGVALNLCEASQVFIMDPWWNPSVEWQSGDRVHRIGQYRPVKITRFCIEDSIESRIIELQEKKANMINATINQDEAAINRLTPGDLQFLFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T804</accession>
    <name>A0A8J2T804_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S07-05908g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_05908g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T804"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244:SF471">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT BETA 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244">
        <property type="entry name" value="KELCH REPEAT DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="434"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="587"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="442"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="614"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF90647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="827" mass="92060" checksum="1A4B15F09617C79E" modified="2022-05-25" version="1">MGDMELHHQAAVTFAISGDEEANRAGNLCYGPNEEDLTMNDAAMGKNGRFWALPYMSNYVSVSAPVFIRDLKERIRTENECLLKRYYSSKKLINYAMHRHGSMTSGSDASTVPNERSKSYVWLHKLMDDSRYMEHLLESETSLELLANGDEGEVKDEGQVLRARVASSYFKYLQKLLAVDLREVEVLKKHNLWIPTLKKQWRNPFSEDVSIDGSLYENKVSPLFIGGVDYISKDYDLYKGSTLIPSLFSEYKLPALVYHCSVEVQGKVFMMGGLMACHKHDDEAPSLKDFVVDGIENLPPPLLSTVVNNPCMVNNCQLYVMSLSSNTLRKPELSGHVPPPLLCMKGCKLTERHIFFYGGFEIRTETRVADNGIFHLKRRAYMNSTGHILDVLTFNFTKVELTALPYKFFSFPTLPARFGHMQVAVRNTGGSYSNRSFDMDDSGVSSPSGGESVDSSEQNLNASSPSVSMASTLARLNTTGHGSGVYSILIFGGYQQSGEDDYEAMNDLWKIDVTVLAKGKRGYFKFGNTANATLIQRPSEVDPWPSPRAFLADCVTDMRTTHNKPDLLERLQQDFCIDEDSFPTEEKSKSIFKNLPHARTDNERGKHRMRSKEGNYNGSSNPFAKQRTTSPPSASPASLQSLDKASKLDQKVVVIHGGSNKREVFSDMWWFDMETEIWTSVTLHGKSKSGMTPISVGLVGHSLVCVGDMAVCIGGLLQHDVDALFFGKSYNHSKPQLAIGSDFFNIFDLSSQCLLGHTVGGQEDSPILLDDPNLRSRMVLSCGCNVIQSNGDIFLFGGIVSRRCDRRAVHLRGAVLKCVLPSTKLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T834</accession>
    <name>A0A8J2T834_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S05-01574g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_01574g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF89781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T834"/>
    <dbReference type="OrthoDB" id="4069593at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
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    <sequence length="1162" mass="131322" checksum="6C8B192DE1BB2594" modified="2022-05-25" version="1">MVSAQVTRQRVHPILRNPDERRTSRDASPTRRSWPSSETSMSPQSQVRFSIPDPQYVDEAQLSISESPSKIVFPKGCEDDSEYETANESVRASIQSLGKDGHFMDMHSRVMIDVPEEIWDFHTRKRLGGHRRSNSSRSQSMDNGNEDLSKSLQSIIVDTLDSMGTYKEKYLAENPDSGNSSSISQMSPLNLRREPKSELYLTTYSPLNNHNVPIPLEISLPPFLSPKNKCKKRNSLIFDGTSYSPFVEDPYEGSEEENVEEASEKRQLTDTEDELSISDDSIPPAVHDISFDVGSAYNIDQELGIDKDANVNLKIQRKNLRKHSPDKKLSQGYRTPSPSPKKLPINTKTKQLQRPGGIPNDEKRPELSKSRRSLNILETPFKQISIPDLEHEPASTTSTGSLKFFDSFEEPELQLQSSSPTTKTDRLDMNYKFPLTSPDSDFIKADNSPTSSSFERRRERLMQQKSLPSGGHRHMHSRSRSIHGVGDMFAATSTPPSKKSPESELRQDYLPKSKPAFSVDDEHYKPKNSKLSDTGQFVFNEEELPVTPLPFEKVESNTTLTCFHPVNNSSQPSDMKSRHTPVSFQEKVYEVPMTPDSEARDAAESDASSVYEDVHQPQKTNVTSLEEPTLRQENGSSQDYSNYNKTDDSVKILSEKRLVDDHFDFSEEFEEEDMVLSTPVKQSRTPSPISHTRPMTSIQSFNRPIIPPKSNYEQIVSMPIEPSRLHYNLLSPRRSLSNLGSQSSKNSEFSKQTNQSSRTSYPIEHEVINPLVNRLKNSDYPSHSEYPPIPRASEPEYRPYEDHVKNLPSFRRQKEENNLIAVDKEFVQQGDSISNNHKIPKSLDRQQNITSIPNKVADGNYPDIYSGLAMSTETGTTGRPESRIIQKNHNAGENYTGEQLRDPSEPNGSQVFLRPHNSHIPHKSIEHFEPEDLYGPQNYQMIEDYQSIKQNAELEEGFSPGGDALGDETSCTHRLAASADFREVFETLNNMSPTSNKFGPAVTENREAIVLQKSANSKANHLVQEDYHTEYEVRDGKLVEILVLNDDSLDSAKSDSGETSLVTPTRRPENGSQNRSHEEILKMCEDTASHAKMIIMELVQEKKTKSKTKTQTKKILSRPLPPNSTEVGQPGLSSSAIQHTTVNRSQGRYLRNLNRARRPRPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T866</accession>
    <name>A0A8J2T866_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_07646g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
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            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
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        <property type="protein sequence ID" value="CDF89034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="504170at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000399">
        <property type="term" value="C:cellular bud neck septin structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0032161">
        <property type="term" value="C:cleavage apparatus septin structure"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044879">
        <property type="term" value="P:mitotic morphogenesis checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="CDD" id="cd14081">
        <property type="entry name" value="STKc_BRSK1_2"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
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            <begin position="89"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
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            <begin position="1"/>
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            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
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    <evidence type="ECO:0000256" key="13">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1640" mass="181770" checksum="E2DF633591A154BE" modified="2022-05-25" version="1">MPVPMYANRGQRPSSVAKKAARNAMFKAAGTAVATRPSTTSKISQQQLDRVVQSVNDATKRLSQPESAMSGSTTTKSSKRKSRDTVGPWKLGKTLGKGSSGRVRLAKNMETGQLAAIKIVPKKKCKNDPGSHSSSKSSESDASAMMNTTTSTNRATTNATTNAGHSNVPANPYGIEREIVIMKLISHPNVLGLYEVWENKSELYLVLEYVDGGELFDYLVSKGKLGEREAVHYFRHVMQGVSYCHAFNICHRDLKPENLLLDKKNKSIKIADFGMAALEVSNKLLQTSCGSPHYASPEIVMGRPYHGGPSDVWSCGIILFALLTGHLPFNDDNIKKLLLKVQSGRYHMPQNLSPEAKDLISKILVVNPFRRIPTREILNHPLLTKYDKFVKPVKYRTSANMPLSQGKSNSDLHVLEEHASKIVKLRSRNEIDESIVSNLQILWHGASRQLIVSKLLKPTMSEEKLFYSLLLNYKEKHSPKPEPEAPATFEPTVTPPQPVQPVKSTKPIQSAESFERAEEVQTSLSTEEIQPHQSVLPHEKDDVEMHEEEVEDEHEDEVEDELSTDDANDEGAANNDIQDTSAPKLQQKSQFSTPSIKQESMSGFPSMNSNALSGVPSLPSNSLPPAMPIFAASSSRTFKNSGSTLSLHSRRSLKKSASKISLSNSSSRRSLHHSISKKSLQPSPSTTTLQHSASKKSLRQSPPRKTLQNSASKRSLYSQTSISKRSLNLSEYVANESAVLSEMEQQLKPNLPSSDSNGDFEKLCEQILFGNALDRILEEEEEDTASSHDNSFVSEKTLTKVDVSSSPEKYKDDGASSAGHPPTLEFSEEHSDIAPRSIASKKAVPDIPERSPFRDITNVPISSGPKTSKLKRDASQKSNLNEVLKPNQATTPTYVSLNIMRNGSRQDTQSRSRHPVELRSTSEGRPKQQGYSLDPRRNATQPANPKVLENLFQKPKTKPSLNSVKTNEAWSSTSGFNFDAKSDMNFDNNNLDNLYGKGFNRLEGELDGEGDNKRIDRLECQSENTNSSYWQPSLRGSQTDLEMEQNSSNSTGLFETSDVNVLAHSSIIQKPTLSRQRSILSSTGTFRNLNEQLQRNDDQITTPNQSNQTDHSNVLRKHSTELTLAPRSTLTAALGPRDRSPYTNSFFSNMSDMSDVMEMPTNTYVAKAVEVSNNSSAEHISDFQMESDRDVDPSSSQEQRTTATFEDNNINIFEDAPVDSESIETTSTTDSQQNVRRKAVSIDTLHTTNVLTPATNVRVSLYMNNNANSESPLQRETTEEIISKFKLSPEKSSQPLVQKRFSAAAANPNSDMHSTMTIFKDLEEGEGEGSSSEQINEGDERKKPNRVTMLFDNDEELPQLKQTSAAAQKQDNVKREEDQGQEDVNPGKTKQSEPKYEEKKDGNEKQEEPEEHPRDKDEKVSQMHERAMQINKSLKRKRKNGPLVTVTDTTASATSNVAPPAAVPAIKPPPPPSAKQLSKKTPTENPPVPNPPVPNSPASTPVNTSSFAKPPHARKQNWFSKLFGGLKSQRKSDTGKLQQDHFTKISFDDAHLLTLHEFDKNAVEYHLKGLEHKLHDEKVEYDCKFPNGFAFKIKIASSVFPGRVSTVITVKKKGRATSPESRSAFQAFNQSVAKVLRDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8L0</accession>
    <name>A0A8J2T8L0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Alpha-1,4 glucan phosphorylase</fullName>
            <ecNumber evidence="12">2.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_04170g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + phosphate = [(1-&gt;4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:41732"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9586"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.1" evidence="12"/>
    <dbReference type="EMBL" id="HG316461">
        <property type="protein sequence ID" value="CDF90831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T8L0"/>
    <dbReference type="OrthoDB" id="9215500at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04300">
        <property type="entry name" value="GT35_Glycogen_Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000003">
        <property type="entry name" value="Alpha-1,4 glucan phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000153">
        <property type="entry name" value="Alpha-1,4 glucan phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011833">
        <property type="entry name" value="Glycg_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035090">
        <property type="entry name" value="Pyridoxal_P_attach_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02093">
        <property type="entry name" value="P_ylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468:SF3">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE, LIVER FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000460">
        <property type="entry name" value="Pprylas_GlgP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00102">
        <property type="entry name" value="PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="10 12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 11 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000460-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF90831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="903" mass="103703" checksum="02864CCE5093CA7D" modified="2022-05-25" version="1">MAPSFSNTNDVIREESRASQLVPRLTRRLSGFLPREIKSIDATIPLRSRNLWKKNNIQKFEEEEGFEKKFVTHVETSLARSMYNCDELAAYEATSMAVRDNLLIDWSATQQRITTRDPKRVYYLSLEFLMGKALDNALINMSSDKQEFSSTGDNTSRGYVKHALDDLGFNLEDVLQKEPDAALGNGGLGRLAACFLDSMATKSIPAWGYGLRYEYGIFAQKIINGYQVETPDYWLNFGNPWEIERNEIQVPITFYGYVDRNNGDTTTLSPSQWVGGERVLAVAYDFPVPGFKTTSVNNLRLWRAKPTTEFDFAKFNNGDYKNSVDEQQKAESITAVLYPNDNFTKGKELRLKQQYFWCAASLSDIVRRFKKSRRPWREFSDQVAIQLNDTHPTLAIVELQRILVDLEKLDWHEAWSIITQTFAYTNHTVMLEALEKWPIDLFGSLLPRHLEIIYDINWFFLKDVQKKFPKDVDLLRNVSIIEEASPERQVRMANLAIVGSHTVNGVAELHSQLIKTTIFRDFVRVFGEKKFTNVTNGITPRRWLRQANPELAKLIGEAIHDPEQNFLLNMSKLNGLTKMADDPDFQTKWDSVKESNKHRLADLIKDLNGGVEVINREHIRHTLFDIQVKRMHEYKRQQLNIFGVIHRYLNMKDMLKNGKSIEEVAKRYPRKVSIFGGKSAPGYYMAKLVIKLINSVADVVNNDEELQDLLKVFFIPDYNVSKAEIIIPASDLSEHISTAGTEASGTSNMKFVMNGGLIIGTVDGANVEITREIGEDNIFLFGNLSENVENLRFKHQYNETELPPSITRVLNAVEKFTSEYKNEFKPLTDSIKYHGDYYLVSDDFDSYIAAHDLVDDLYHHNKHEWIKKSIISVANMGFFSSDRCIDEYADTIWNVEPLKPESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8V7</accession>
    <name>A0A8J2T8V7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">eIF-2-alpha kinase activator GCN1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_06458g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GCN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5148094at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904688">
        <property type="term" value="P:regulation of cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000090">
        <property type="entry name" value="eIF-2-alpha kinase activator GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022716">
        <property type="entry name" value="Gcn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056810">
        <property type="entry name" value="GNC1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057546">
        <property type="entry name" value="HEAT_GCN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056809">
        <property type="entry name" value="HEAT_GCN1_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346:SF7">
        <property type="entry name" value="STALLED RIBOSOME SENSOR GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR GCN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12074">
        <property type="entry name" value="Gcn1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24993">
        <property type="entry name" value="GNC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23271">
        <property type="entry name" value="HEAT_GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25801">
        <property type="entry name" value="HEAT_GCN1_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24916">
        <property type="entry name" value="HEAT_GCN1_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="5">
        <location>
            <begin position="1319"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="1416"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="1495"/>
            <end position="1533"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="1607"/>
            <end position="1652"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="1652"/>
            <end position="1691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00072275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF90349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2675" mass="296268" checksum="FD1D129C39D2E82D" modified="2022-05-25" version="1">MCSVSLSDLSQEELSFALSKCVNESLLANRLDFLKELHQFVLEGNLETPQLSQISSTLLKSYSIFHEGKSKKLVTCILLEILKLDSRFLTQYGQFILNQVTPHPGSKAVSDYLNLLEWVHAFYKVITLEKEEFPKHVSLLLEAHFYITFGIEDNLDNQEMKKKSREGYNHHRERIRRAMLQSSSKTFVSCLKDNHDALFYFDTVGEETLRLFKAKKAPVVGFIVVLGALCQASVQLLSSHPTLFHSLKENCASQFCELIGKDVILGKNPPSELCLKTNLAPYMKDFVTEEQFTAVLVPNLEKACLRAPEKGFKIAMVLYAGVNSREVNLLHIFVSTKLISQSFSSFKSSKENVRHASIESVLTLLSCLCIENTSSDDANLLVDEIFKNIKANMNTEYKSLACKVLSQIPNVSDEVSTKILSGLSTYLTKEVNEIALESMLDCFFTNYFALKEPNESFSQIIANGFTEKKPNLKKLWLSSFTLNSKFASDSVLQQFGESCVEFTNETLSHYQRHDHIAMFACFEFIDKVYDSQLSNVKLKLGNLMASFSSPCLGDSFLQISLSTSLSVEKRMRSVELLEKFFRRDPELIGCNIIEALEKRVQLPGFPESEEAISFKFIVPVFNAISKPVPDKAAIIKVLLKSFIISQCFEFNLKNGWAGLVLNAMLDPANIMQKHGETIVDDALTVLNNPKMADTIYGSCAVQALSYAAFINQKAISPLLAKILQNDLEVKTIAHFAQEDIAIWKGNENEMVFNVLEKDMDKKLADKNNKDYETLKWEQSIRKEQAKKSNKKLTKEEQELIKQELAREAEIRKRVTEAALKVNRGVRLVHQLAQDSIQVDTGIEIWLPIAVTKLLELTQSSNSAVFLGSAALDAFLCLSSDISNRLGSMSFYVGLATLRVKNAANIPENFLEEALPELLSRVLFRVKIVSQQAALDSTTLIYMLPLLIDVLQEGKRVALMNANKPISNSEFVEEAREEEHLLLAMDVISSHAEVFSDPSIPRDAIMHVLLSLLALPSKAKMAKACFNALCQSISLAPTTKDLQLLLSSTLSSNQFVRSSVLEVIDNEFELEPFMSFSAEIFICKQDADETCRSTADFIWDFNKFKVSEELLTTLLTFFHQSDNGLRLFTAKAYAAGVECLQNLSPVDVTDAHLSILMDFYLEKAKALEPIIDNFGLVVVPASERKDPWEERSAAALAMKQLVSGISKEGSSLVNFITFLIEKGAIGDRKALVRQEMKEAGIEAIEKHGSKKVEDLIPVFESALSSKCESVVKENTIILYGSVARHLDADDQRIHIVIGRLLATLETPSSEVQHAVSECLSPLVFLFEQKVEDYVNQLMDKLLDTSVPKHVQKGAAWGIAGLVKGYGISSLSKFDIVRNLIEAAEDKKEFKKRESVAYAFQYLSISLGKFFEPYVIELLPNILKNLGDSVPDVRNATAEATKAIMAHTTSYGVTKLIPVAVSNMDDISWRTTRGSVELLGNMAYLNPTQLSSSLSTIVPEIVGVLNDSHKEVRKAADESLKRFGEVIRNPEIQKLVPVLIKAIGDPTKYTEEALDALIRTQFVHYIDSPSLALIIHVIHRGMHDRSANTKRKACKIVGNMAILVDTRDLVPYLQQLIDEVEVAMVDPVPGTRATAARALGALVERLGEDQFPDLIPRLMDTLSDDQKSGDRLGSAQALAEVISGLGLPKLEEMLPSIMSGVTSYRSSIREGFMPLLVFLPVCFGAQFAPYLNQIIQPILAGLADSEESIRDTALKAGRLVVKNYASKAIDLLLPELERGIFDENERIRLSSVQLTGELLFQVTSISSKNEFSEEDNEIGGQVTVKMVEVLGQERRDRVVSALFVCRNDTSGMVRASAVDIWKALVPNTPRTVKEVLPTLISMVVLHLASSSGTLRHIAAQTLGDMVRRVGGNALSQLLPTLEESLKDTSDPDSRQGVCIALRELIESSSVESLATYQTIIVNIIRDTLVDSSECVRKAAASCFDAYQEISSKTAIEEVLPYLLNLLETSSVSDCALLGLQEIMSTKSEVIFPILIPTLLEPPIDAFRSSALSSLSEVAGSALYKRLSTIINSLVDAVVSTASDTATKQSIKAALSKIFLSVTDEEGLHPLLQQIMSLLKSDDFEKRLVVLEILPQFFSETVLDYDVYTSDLVSNSILSLDDKDPRFVQASHDLLLALISNEDKSKFDRLVKPAKQALQMTGKPGEDLPAFTLPKGPNCILPVFLHGLMYGSSDEREDSALAIADVVSKTPAANLKPFVSVITGPLIRVVGERFNSDIKAGILFALNILFAKIPQFLRPFIPQLQRTFVKSLSDPSNETLRLRAAKALGTLIEYQPRVDPLVIELVTGAKQATDEGVRTAMLKALLEVVTKSGLKMNENSKVSIVNLVEEGLMSSDDKQAAAYANLIGSISETLSAEEAQRILREKVLEAGLEGDTGKFGVLALNSFIKDVPAQVVNSQILDDIVQYLLDAIRSPSAYFSENGLLAAGKLLLLQGEKKSPYSKIESEDPLELGEKNIELLVAEICAAMLAPVSTSTDARRLTLVIIRTLARFKFDECVKPFLNLLGVSVFSCLRDVIIPIKLAAEKAYLAIFRLVEEPEMTTFNAWFLELSTQNSTVTNAVGTVVQLRSIGDYTRRVGKRLANVERERIAEGGDAETMFSDRFEDEREIWAVGGLEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8Y7</accession>
    <name>A0A8J2T8Y7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_03224g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="HG316462">
        <property type="protein sequence ID" value="CDF91029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T8Y7"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045033">
        <property type="term" value="P:peroxisome inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000397">
        <property type="entry name" value="Serine/threonine-protein kinase KIN4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="37"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="330"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="528"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="567"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="621"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="676"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="760"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="340"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="567"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="632"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="680"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="719"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="774"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="797"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="836"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF91029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="873" mass="96406" checksum="48141CB5978888CB" modified="2022-05-25" version="1">MASIPQRHTYYGGETSVYTQSETTTRIRRKHVTFGPYILGSTLGEGEFGKVKLGWSKAASAPLEVPKQVAIKLIRRDSIPKNSEKEIKIYREINALKHLSHPNIVRLEEVLQNSKYIGIVLEYASGGEFYKYIQRKRRLKEAVACRLFAQLISGVNYMHSKGLVHRDLKLENLLLDKHENLVITDFGFVNEFVTHNELMKTSCGSPCYAAPELVVSTKPYEARKSDVWSCGVILYAMSAGYLPWDDDPRNPDGNDIVRLYQYITRTPLKFPEYITPLSRDLLRRILVSDPRRRITLKNIQRHEWLKPHSQFLSITPEEWDKAMLAKGVFRPPRPVHTSKRPQSSCSTSSTGSKGEKRDSLVMDYTLVPLPAPPHQSQSHVITKPSPPSPEMRVSPVRHNMNRHSRSDSAASIALQAVVNAEREGINTIGSGGGNAHNSSVGTRANASGDHMSANINVSGTGTSTNRNVAKFVIQRSNSVTGSVSPLINHDSAIMEKGLLAGKSLEAPQPNRNITPLLNDGLIHSNKVTHRKPRPTSYHPGSMAANRSASPGTMDTLTGVANMAASRAMSTAESSPLDSAPSLMPESGLNSQEHSPKMLPRRSFSVSEPSLDLQSVSGDLIKSHGDAQPMARRLHEQVEDVDYRQRRQSRRYSSFFNSVTEEGEDVESRIVPVHIVPGGAPPRISTTAPSASPSAAPSRGHSRVPSADINTPPNGIPSGLPSQSVSLSNSELVASRPEKDRKQDRKRFSFLSFYSSYNASRSSLVSEAPTHRRVGSQTSQRNSLSRPATRGSVRPSTQSSQSAQPAQQHQQQQQQQQQQQPQSLIAARAGRSKKNRQSAVPSIEGTQSPPLRPQGEQSTARKVMDFFKRRSMRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T922</accession>
    <name>A0A8J2T922_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S07-04720g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_04720g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T922"/>
    <dbReference type="OrthoDB" id="3973067at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026241">
        <property type="entry name" value="GIP4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02082">
        <property type="entry name" value="GLC7IP4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="610"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="788"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="448"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="566"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="579"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="610"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="640"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="658"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="702"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="720"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="790"/>
            <end position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF90596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="834" mass="93111" checksum="A35013E0DE0F281C" modified="2022-05-25" version="1">MFAVAAAHATLPAKIKGTFQMDWHDVRIMQYKAAILKLEEIVRVLKILEKNLDKKQEVGKCIIPLVNYMLSLCDGPLFNVHPLLRKRFLLLNEFKLCKQTEVIPPVITSGVEFHVELANIVKDLDQLRGSIYNVGLQWKLLSCLQFIAQNAHEIYEKKLIQVQIERTANRPADAPGIVLPFDLGSVEDIMKPAELSLCLDWAVLINDRDQDTTFKSLRKLQTQILTKFVTNMNEKALPVLRTYFNQLQKSSTSKGPATATIKELPHWEYTIHRMYAYIYRALCLLDVMVSLSRQIYLPNKSYLHSLRSQLASENVSAYREILEKMDSMCADPKSEVTGSILASLDSCSNPRVNLCVAFNTVTDVFQNSICSVTPILRNTIQLISTWLDMWKFVDSNSDSAQKLANLNDEQLENMLKERLAVDKLNQVEKQKTINRLTPEANGLGGRGSSVRRVSSRRLSAPSSPATSSSGTSPGKMSPLRISRTNSRELGSQRSSNYSSPQLSRRSSLAEIRTPGIQLSSPQISRRSSFADNRQPNPASILMKNAIDAKNQTRSSVGNKTLAGRPRSSSLQTGQEKASKTQSTTSLLSRSNSLQSSAVVNQKVVQDNFSQLTRGSKATNGPFAENNTKKLLTIPSRKPRIQSSNGALTVVNKQGSKGASKTATAPETAKPTLDPSKAPTATEKNSTLKTVRIEISESQPQPQKEESEKRQKESKSELQQTQVSLPGSPNVTCMKKVRFVGVSPMTEDEIPKPTKKGWYKKPAVLHYPPPPPQYLSQKFRMRQEGIAFRSSLREEPNQDSSRRNSLVSSSEVIPLPTQKESVSQRFASKLRERLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9X5</accession>
    <name>A0A8J2T9X5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="16">ZYBA0S09-01552g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_01552g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316462">
        <property type="protein sequence ID" value="CDF90956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T9X5"/>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07543">
        <property type="entry name" value="P-type_ATPase_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000049">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000071">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057255">
        <property type="entry name" value="2TM_P5A-ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047820">
        <property type="entry name" value="P5A-type_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF7">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE HELIX TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23143">
        <property type="entry name" value="2TM_P5A-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01229">
        <property type="entry name" value="COF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1060"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1138"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1178"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="domain" description="P5A-ATPase transmembrane helical hairpin" evidence="15">
        <location>
            <begin position="21"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="14">
        <location>
            <begin position="258"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="913"/>
            <end position="935"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF90956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1216" mass="136008" checksum="4CF081094C0A719A" modified="2022-05-25" version="1">MPPKSLVASPLVRDSRLLSPKPFLSRPYVLFFLPLYATFAHIYYKQYDRYIKGSEWTFVYLGTIISLNVLVWLMPEWNVGIEAGFKFITATELAEATHILIKTTENNGADDIVEIHRVEEDGVLQIFFQFQKKRFLWHADEEVFASPKFLIDEPPKLGLLQKWEGNKGDLSHLRRLYGDNSFDIPIPTFLELFKEHAVAPLFVFQVFCVALWLLDEFWYYALFNMFTIVSMEAASVFQRLVTLKEFRTMGVKPFPIYVYRDNKWTSIESDKLLPMDIVSVTRTAEDSAVPCDMLLLDGSCIVNEAMLSGESTPLLKESIKLRPKDESLQVNGLDKNSVLHGGTKILQVTPPETQSHLQPSPDKGSPALVTKTGFETSQGSLVRVMIYSAERVGVDNREALFFILFLLIFAIVASWYVWVEGSKMGRIQSKLILDCVLIITSVVPPELPIELTMAVNSSLAALSKFYVYCTEPFRIPFAGRIDVCCFDKTGTLTGEDLVFEGLAGLSKDPKDIRHMYMASEAPERTSVAIGAAHALVKLDDGDVVGDPMEKATLNALGWIVEHKNVTTKEGLGKITIQRRFQFSSALKRSASIACHQGKHLAAVKGAPETIRQRLVKVPENYDAIYKSFTRSGSRVLALASKALPKLNQRQIDDLDREEVEEQLNFDGFMIFHCPLKNDAIETIEMLNESAHRSVMITGDNPLTAVHVAREVRILERETLILDYAENVQSNKLVFRNVEETITIPFDPESGNFEQDKIFAKYDVAATGHALNALKDHAQLRDLIRHTWVYARVSPTQKEFILNTLKDMGYQTLMCGDGTNDVGALKQAHVGIALLNGSEEGMKKMAEQRKIDSLKTVYEKQCDLLQRWGQPQPVVPDKIAHLYPPGPKNPHYLDALEKSGATITPEMRKLVEEANSKPEQAVAKPADTPRPTKDDLATLLSSAGAGAGSGDDDVPSLKLGDASCAAPFTSKLANVSAVTNIIRQGRCALINTIQMYKILALNCLISAYSLSVMYLAGVKFGDGQATTCGILLSVCFLSISRGKPLHKLAKERPQAGIFNTYIMGSILSQFAVHIIVLIYITTEIYKLEPREPQVDLEKEFSPSLLNTGIFIIQLVQQVSTFAVNYQGEPFRENIRNNKGMYYGLIGVSLLAFCGATEFIPELNDAMKFVPMDDDFKFKLTLSLALDFVGSWVAEHFFKHFFMNDKAADITHRTIQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAA4</accession>
    <name>A0A8J2TAA4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ZYBA0S14-00540g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_00540g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endomembrane system</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316467">
        <property type="protein sequence ID" value="CDF91765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2TAA4"/>
    <dbReference type="OrthoDB" id="10070851at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd07609">
        <property type="entry name" value="BAR_SIP3_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13280">
        <property type="entry name" value="PH_SIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039463">
        <property type="entry name" value="Sip3/Lam1_BAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042067">
        <property type="entry name" value="Sip3_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14248">
        <property type="entry name" value="CYCLIN Y, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1040"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1069"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="311"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="9">
        <location>
            <begin position="772"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="252"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF91765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1232" mass="142087" checksum="EDDF5E50C57D8922" modified="2022-05-25" version="1">MATRKKDKNLESKRLKLISVAYKEASLDTPSFRASVNHFQTKVELLEDWIEKTVGFTENHYNVAMDDFNRAQSTFLTQLLPSPIMLSNGFVDNQLYTPWLVESFNNDYAEFSAKLLKIISGLDSGYPNALLELMNNYIEPYKSKRANFEYYQNKHDGMLNQHQAVNISLPGVTPSSIRENALQLFEVRKGYLDASLELIGTISKMKLGIDKFVMQIIGLAQLKSDYTFKESGKKVDLTPLLKENFVAYSDWVENSMEAAQSLQQEMENAKRQVLQSTAYRLAPSRDLNDYNVKAINSNILQYNVIETPKESPEKCGWLYMKTSLSSPQRDIWVRRWCFLKNSVFGIFLLSLSKTYVEETDKFGIFLTNVEYEPNEDRKFCFGLKIIGDKTAETDSSKELNLTLQAESLEDLKSWLKAFAVARKYASRLEPNSLEYDMAFQRFPPEFFEFASSTTTSMDQALTSFSSHTKPLVDILDEALTGNASTFSPEGKLCHFSMAVTPMATKLTQVAILANFYRKGNWLPDAVVANMWGSSDWSGYAIFDEGLLHLSKTKNTRFEINTNSKQTPYMKYPDFYPPEMKVSDIQFKSLFFTIDENLYKFPDDLLLFNFSGFWCPNKRQKFSATCYVTANHLYCYMNSMGFISLTHLNLQDLVSVEEGKSSGNVIKIYNADGIQLRMYIYFKDHRLVAEKLQYLLENKTRVHPKDEQEIFRDFELIDQKYQEKLEEEHAKMIESGSNNAIANNVPNDLAATFWSMSATAAECFGRSKELQKKASITYQHTYDVSPKTLMHILFGDQSNAFPRSFFLANRHHPYNYVSEWKEEKAPHAKPALVREIRFGFSIADRSLGDANYREKAKGAQGMVKQKISKILEGKFYEVDQDPFIVKVVFCHPLRVTTQYLIMEPHETDTPLISTSSTGSLFFVYYRLEFIYGNSGEVISSLSFVERTVLKWAFRFSQMEYIYIKKIIRYYMERIGAHGKLIKAVKMCGKIGVLSPEKDPHKKDKIAESNSANDNDREACNLVEYNFSIVLRIILKATLYRIVNFTFICLRAIVSLIFLIGSGLSHINNTLAIALILSVGLNVLLFGRSTITYWLVRRAENTFRNLNQANNDNVMQRAVSIKDLDLLTTDLAYETENLAFNKFEEIDRKNDYIYKDTRQEMAFRRNELLVELKILQDMERELVRGDYRKFLLAEETQCETVKNDLIDVWDNATDLRRYCESCNMELERLTSLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAB9</accession>
    <name>A0A8J2TAB9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DOCKER domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_02674g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316464">
        <property type="protein sequence ID" value="CDF91430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="18896at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11684">
        <property type="entry name" value="DHR2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057500">
        <property type="entry name" value="C2_DCK1_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032376">
        <property type="entry name" value="DOCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653:SF10">
        <property type="entry name" value="MYOBLAST CITY, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25338">
        <property type="entry name" value="C2_DCK_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16172">
        <property type="entry name" value="DOCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DOCKER" evidence="3">
        <location>
            <begin position="1404"/>
            <end position="1819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1821"/>
            <end position="1893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1821"/>
            <end position="1834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1856"/>
            <end position="1881"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF91430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1893" mass="215256" checksum="E43EF2A0B87A7B54" modified="2022-05-25" version="1">MADSSGEHAWEPTQGLQRAVVVRSFLPLKRYPNLAATNDHFCNLYAGDELYVFETTADRKWLRAYLCVIPLPESYISLARSGSLNDGLPQIGSKLVIVPRRFVSFSVDYEVSPTSFLKMPNSADLNADVSPECKVPSLYDSYSANDVTAKSGTKPSRPPFPFIRYQRSGLVDELNAAQGLLSAHIYRMYSMGEFFVYRQMTLLYHQLDSITLRLKFGLVTQTERTKVIRSATALLTNISKLISTKTKSKSLNGPSSSAFNPQHSDPAGFESVFARDADTGELLSYKESSLHKLMVNTMLQGLSKEFPVSNRKLFEMEVSPNPLFDSSQSHILVDVTDVTSDASIGNPLFNNLTASMYLSTKKEVLTEPFTVNIDSEHILSLNNISAALFKNLPDSKTQRNNIYLVVVLTEKIPIKTFEINPESKAADQSPFIPFSLHASGASIGTVTRGVAAGAVDITRVFSKYNNSFTPTENSFKFKIYLFGSYFVKPKHDENKPNQPITGWGALLDNILSNSLQGVAVNPRATSLSVSVKEINGEAASQRSMGSLNSVIKSVNTHFYDIMSQERERIYLQLGKVSFCNIAPGGSNVKNVTIQMSSNNRGVFFSRGSTETPFYRWQFVSVEPGEPIGDSVKIHGIENMREEESIKVLAYLNGVLMAKADLTIKRHNQIVEYKKYSTIELLNPHKEPLLNLEVQSEYVGYRYNMDPKLQKFLNIQSYYKTDNAAFQQDCTNVFAGLKSVSLNQFEKYFAKILQNFADLFYLFAISKKKEFPDSLSAKTFASFVTFIDKILTHNEGFRYSFSKFYKKLLKSSRVSPSIGFVILDSMADVFSKSHETWDSVGSAVARNSIYLLMLSVAFSRGMDATWRQSFERVFSQVCEFMTDTSDKTVVDQTAILEQYDLWLDCLTLYYPPEEIVQFTLALLQSCRNKEENLDFFIKRLSAREEKYANTKFSLLRRLLVHRSLHDYLFVTGVAQRLRLIFLTKCIEWTLQPYAVENIQLDTARLGNGVLITIVESTTDRMLQRNLLRLLPTLCKVFLNARKHCQENNFFKPKITFTRLFPFSMPCTVHSMDSLIKKEIVVEPLLEIVTIICEILKIAEKLYGANASYTEILEECGNKELHSKFFVQSISALDIFSLYHVIGIALKGGFFPAKKWLGVSAMIMRSLMTLLIMYRDCMTKGNGLNTVDKNDAKLMAAYLKSLFLLANHKTTFLVDMAILPRKAVYLITGDSSERAAKLLEFAWEAIADEEYDHDCAKRFGFGKIGANQLALLDENVPLIGEMMVFGAHDHFEAIKVSCKVLWYTIINFWMAYGALKPILDATIPELYNNYTTGRLRLTDDELKKLKLSMFYTIHISPEDKSYESVVIAMEELFSFLQLIADADKIPDEAEFDDDRTAVHMEMFGYLMDANRPELFQKMIYHLFIQFTKKKDYVQAALSLELLANTFDWNPNNRLMAISAPPMPEQSSFERKEYLYKEAAFNFSKGLKLEKALSLYKDLTTAYDHINYDLSGLAFVHNEISNLYTSMQTVDRLVPTFFKVSFAGLGFPSSLRNKIFIFEGMPFEHITSMCNRLLKLHHGSSIVQRFEQLEELFVKPKMGKFIHISSVEPQLEFSDEYQSQNDRKGKFNYKTWLYVENKNMKTFSNSRKLPGPTSVTDLWVDEYTYETASTFPTLMNRSEVLKVAKNKLSPLENAMRSLRLKIQELSGLADMCYKVYRGHEDQTMWFNELSRNLTGTIDAPVNGGMTQYREFLKEPVASQFDANELGNLTRAFNDLAIVLAHCLNVHRELLPSEQLKDSHNMLVVLFKKNFEPEIKKNHINFDSTSLTSTQSHGSSSFHSRKNPFAESSLDPSLEYSEAVSENGSDLQTRSLAQSRLTWNSNSSPPMMFRKKSHQRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAE0</accession>
    <name>A0A8J2TAE0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S10-04082g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_04082g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HIR3 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316463">
        <property type="protein sequence ID" value="CDF91294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="77564at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000417">
        <property type="term" value="C:HIR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033053">
        <property type="entry name" value="Hir3/CABIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15502">
        <property type="entry name" value="CALCINEURIN-BINDING PROTEIN CABIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15502:SF7">
        <property type="entry name" value="CALCINEURIN-BINDING PROTEIN CABIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="308"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1587"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="333"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1587"/>
            <end position="1606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF91294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1656" mass="192714" checksum="1822D5576B4C044B" modified="2022-05-25" version="1">MSTFNALNLTPHDEQLEAEEHSRELEIEECFKVFQSALFELKAKRFDNANAKFEELLKRDVIKPNKWGFYYYSSPTLDRLRYLTYRNRGMYHYSYLQENQSTMSSQDIVDCILKVVENLLESIQHNEADSSVTELLIQIFQSYKSKRLERMVLEYELTRSENTLLLVGRKRKSILPKQQLIISQFRDLLENIKDNESHVGFFQSLPRLKEQPRLSSILLRIRDMKTQDEHTMKELDVFEITLQSFTWEALANSLKDLLPHTKISNLLNKSFDPYADVPEPIEAIRFVLDATENTEVNTDAKKIAEVTEIEEEAPSADSSKEPKVAELNSVKNVSEEHNISKRPIEHADAPKPVQRSSKRFKDKDQDLVENDLLNTTHVAFMEELANLSEMLGLRVKLNQWKSLLDGQLQDDLTFLPYRDLLECLKGWSSWHTDIFTQNDSSSSGNNQNDEETLQLNALLKSNVFADKDNDANLDDLPEERLMSFISTVNKHATHFQEARFKFLKLLLSLDTDQRTRLIVDCSWSRSLFEAVEWLFLSTEQNLFEFVSSNSDICPYFALSFYELLVNMMGNICEQISSKKLQGHRTHELKTQKNKLEVKHERWLELLKNYENPNRIWQIYFQWAHYCFLQYTCEVFDDRIPSTLILIEKMLRESDEPIDIVYPNYRYILRLNLSALDSQIRKINIIRRIGIVDVSNEDTSTDDADQNMEILERILLESVHPAKELSDDDQEMLSFIKNSPFLLKLKLWEALFSYYIRNHNTHSSLNCYFYVLKMLDDILTSEKYSQQSEKQRQQMLLTSLITVGNLTSKFIKLLSFNNWDDIEEIKENNECLQLLLNFFLLFDSVLIFEARKDIFNRSFFQKATKSSARLKDAIANVTTLLTYFFNLHVSHKYPDQHGYLISNFIWCTHSLLGSFGFCDASNGNFLRLSEHLLCQFINQESLIQLKQIIWCRYHYLIAGDNFTPDQHPTKAIEMDKRNSLPLGIYLIKLQYQGKNPLLASGNKASLKPVLDNIIDLIGDPESLGNYIVARNKYLVEEYLEMPLTATLLKKAFRGQGQLDLTTPNDELQEATDAGLFYVSSIQAFNLYRFRKKSMQARPSELDYIVAMLKNDIIYNTQRFESWYLLGRCFAFMVEDDLIWTSDKLSVPEKKSSTAQTQKQAILCYIMSVAVYFARPCNSAEDGPIIQRILGALGLEMMSGYLKPMEKQCYFGAPWAQWMKLNENGELIKQTTANVRTISDFNVEQAILLSLQQAIRFCDHSDFTDTANSFKWIYRYNSGHFLFKIARLDHGLTACENILQSCKIACKISSQKDPIIEPHYSFVNTCYKLLKLGVLSPLKALEMLQKDKDFFEEDEHFWELDISLAVDYQRKVVFQKVINMLRYLLTADKKKWHHRPRYRIAKILFEDFDDPSAALDEMESVMSVKSAHKNLVNIWKPDFERPGKHFVYTYQYVMFYLDLLFCKRDFNSIGRVCKKIRRFGSGMAYVNKATEHALMLYTQCTRAKLQLDEKECIEHLLPSLNYQTFLIVSEDLQNTFTEANYSEELLCGLKISYQLKKGNNGIAFDGICISIYFKYFYLPIAPANTEQDVLSGGQEQDQYDNTESQSQTAERHFKAGSLTPSQIAGGSSSNKYTVPRKRVSKKDVFDKIRVLIEKITCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAQ8</accession>
    <name>A0A8J2TAQ8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">THO complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_01090g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the THOC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316469">
        <property type="protein sequence ID" value="CDF91966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="29024at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040007">
        <property type="entry name" value="Tho2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021418">
        <property type="entry name" value="THO_THOC2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021726">
        <property type="entry name" value="THO_THOC2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032302">
        <property type="entry name" value="THOC2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21597:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21597">
        <property type="entry name" value="THO2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11262">
        <property type="entry name" value="Tho2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11732">
        <property type="entry name" value="Thoc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16134">
        <property type="entry name" value="THOC2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THO complex subunit 2 N-terminal" evidence="8">
        <location>
            <begin position="26"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunitTHOC2 N-terminal" evidence="7">
        <location>
            <begin position="659"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunitTHOC2 C-terminal" evidence="6">
        <location>
            <begin position="939"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="354"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1250"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1401"/>
            <end position="1579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="378"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1250"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1291"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1505"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1538"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1552"/>
            <end position="1563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF91966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1579" mass="180898" checksum="7D96E205A1730FA2" modified="2022-05-25" version="1">MSGSLCATIDRLVLSDATFSSEPRIFSPDFINNWSAESQELCNKFRELQGSDERQKWLKDVFCELFDIIYAQNSEGPMVTMPQVSKLIEDIIDSGAKEVKNCELLSLVGKMFVAVSNSYSDEDENVVLALVRITKSLHKEMFKFSRLSTKLMNHGQTVLLKHLLKKSKYELKKFNLLAECSTGYAQLAMLLVAAYCDPDKLCKVGFWVQQFEQLAGKYSLDPMRCLDVIMDVSSEFITEEYAFLIQFLKSSDYWPRYHLADNQRYESLSQGGNIIASNLITFHINQLGPACETNYLDMVCILIQNGFVSFLSIWANLKPEDEILAKFFKMFDCELEAESKKGAENPLAMADALTSEDDLPTGEEDDKKQAKEAVTSEENEKGIKKASEEEQEASSRENTRKEVLQGGRLAFLKRLLAHGCIIPAFYVLQVHPHFAHIDDFVPQLAARLLEVMIDPLYCSITFNNSGSLDSPLPLTTLENGLLSTKVRLFRQKKTHNPFSAFELGIKSIFYYLEWSEHLSLIQNIDELFAKSHEYLHILGPSLGKAPKLISKLCRIGSEDIKLAAHNREEVTEKWISYVRKFIFPAIPVLGIHPAITSEIYELMKFFPFKKRYFLYNEFINKTSQDFLLAKVGFNKAERKARTILKALSMDTIDIESRRLANLISTNPLATLIPTVKQIENYDKVSELVIITTKYFNDFAYDVLQFVLLLRLTHNRPVMQTDGVNQNMWVQRLSVYIAGLVKSCPGMDITNILTFVVKTLHNGNIVAVSILRELIATVGGIRDLNDVNVVRLAMLNSGEPLKQEARRLIYDSRDTNAHLASKLVNQFAKLDSISELILLLYNLNKEANTGSAHYKILSTRCDEMNTLLWSFVELIKHSFTPEQFAANILSLDVLKNEFDMSISWAFHIWRDYIDQKFKEDDPSADKMLNKVVFEGVDFSCLSKDLFLAFWKLSLYDVQFDRSLYDKKKSVLEEELSNSSSARKKNEYSEQVKNLLVNCISHQKTFNNSKSLIAESTQLWSEDLSDNKIMCFLQYCIVPRVLFSPPDALFCKHFLLQSFGVEKTMRILGMFLSSKILSTLLFCCTRSEAGNMGIFFAQLLESLENERLKKTSEFPYKRDLYDCNALLTGQVIQLLLNQNYMSIRNGIEFMKHVSHVAPVVDTQIEVVCRVLEKILASEDREDIKLPSNALLGHLRARLKKSCKLEEFCEMNEQELQAKNQLDAEMEEIHHYENLLANEKKEAELRKHLELNKKHREQAEKVKQREGSEKDKCTENEQEKEGVDSKKISPIPTGPSNEKQLRPQASTWPFGKVIRFMDEVCYHLSKNNLSRAADCVSDPAENQSLKRLFKENMPIRDFRNSLFKIYERFFRSLVYYPNNLEFTRKLDEIKSAIKLVANDSSKARGDLYSEPLPTEPAKKRSRYNNSDSGRSNGRDMKIESNDGAPGNWKRGYDARNRPLSRGVEVKRSPAAPRAMSFPERPSQPRSETRSGKGYIPREQGGGSRMRSKHEAPHSSEDRSSKRYKADDSRNKMRLSQNDNRTPSRDSRKELDRSKFNTAKRNSTQKLPQGPKGSGEYVSRYQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TB18</accession>
    <name>A0A8J2TB18_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S14-01332g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_01332g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316467">
        <property type="protein sequence ID" value="CDF91797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2TB18"/>
    <dbReference type="OrthoDB" id="5563754at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058155">
        <property type="entry name" value="Skg3/CAF120-like_PH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25381">
        <property type="entry name" value="PH_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="88"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="462"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="698"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="928"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="462"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="474"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="493"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="565"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="756"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="832"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF91797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="982" mass="108369" checksum="F8D51801D2823E46" modified="2022-05-25" version="1">MRIFSGAKTTDFPTPPKIFAQDEPSSGGSSPTSRRSSFAQAFSSPKLFSGSSSPKISPTLPQENSTLENLSPELVSIVTLLSAHAHRRYCEGVFLMLQDLKSDGSSGSRKWEEVYGLLAGTQLASWDANELASCNSNESKPKLKQVASKPRYVNFADAIVRPLDGSEAVITESNEKLQNTLVVSTTLKNRYFLQYSDKDSFNRWNAAIQLSLFECTSLQEAYTGAFLSSRGGKLGDIKTLLADSKFDYEDWVSVRFGTGMPWRRCYAVIYQSQSRKKNALGHISFYENDKKTKKGQAIATIVTSNRLYAVYPSSPLLIDTSTIIKLEGTISFEKKDTPQDTNVFIMPEKHQAVPGYDTIIRFLVPAMNAFKLYGRPKKLIANKEDFRSLLFGLPTLPHVHYLKVEELLPLASSDASSSWTSYDWAVNIKEVLQTKMSNGYSGCGTSAGVMGAMSSPVISSFSLGESSPSLLPKIRPDRVDRKNKANLAPDSPLSLSKIGSDNVSQVSNSGLGKTESNNGLQLQTTKKNEVESVAATSVYSEHERSTSKSPGNISEKTSGRENRNNRNSPDSVYPLTDNNKFTGNEVNPKRNPTTKSQSELDNIYHKYSLSPFGKDNAANVSPQVSEHLFDNPYKHHTDEEKLNIPKLPDSAATTIDEEREIGSSIEGDGEVDSINEEQDALREFNDLAKRINELNIGATPESDREVPLDKISPWDTDLSSTEVTRDTEDNVFDPDFMEQAQMLETDSRHTGAESVSDVSSEGAQTWQDSREVPTNEMSNLRQVSEIEVDRYESMPNPGRRRPPPQAINTNSYAQRKKPAPLSAAPYGHRQQVPPSGNHPQQGYMQPMVKSGGFPSNNYQRPGPYQQHHYNPHNPSPYNPQQARPYGHSPAQQHFPQHAGPYQRYYNGPPPQQRMPLAGVPTAGMVPNGMAPGGVAPRQQMHPQAGRAGPYQPPLQQMTKPKPATGAGFSKYMPSAAVNPYAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TBJ5</accession>
    <name>A0A8J2TBJ5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ZYBA0S11-02894g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_02894g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316464">
        <property type="protein sequence ID" value="CDF91440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057283">
        <property type="entry name" value="RGF3_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052233">
        <property type="entry name" value="Rho-type_GEFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572">
        <property type="entry name" value="RHO1 GDP-GTP EXCHANGE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572:SF1">
        <property type="entry name" value="RHO1 GUANINE NUCLEOTIDE EXCHANGE FACTOR TUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23582">
        <property type="entry name" value="WHD_RGF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="4">
        <location>
            <begin position="464"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="710"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="5">
        <location>
            <begin position="937"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="145"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="196"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="769"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="114"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF91440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1309" mass="148731" checksum="F6076AFB2F1B7B5E" modified="2022-05-25" version="1">MGRNGTENHANVPRSSRGREHRISLPKLPPLDTRYPFLDDSDSNTQEDMSIGWTPVSDQQQLPSRQLPALPPEPVRSLSKLSKAESFSSSPTRSSGSLKKHRNRRPPPPVISGNLASPNSPYGVSSPSALRSARVSNFMGELEFAPTPTSFYNKPPQEVLDSDITSRSGSSRHLSTKRSNESLPFISFDNTDYSTRALPPLPATNSENARSNPEDSSDFYKKLAPPQAAGARIASTHSKFSDSIGSYYSDSHYAFNNSTARNSSFNSLLGGKPLDLAPSITAPTQPFTIDSLDESKLYQCYSVYRLSDIYEWILKVYFEWFNEYVFGKIEFFQMVQLLLEFQMPKNFDQDTIDSNVDRIIESLVVQGAVRFDLEENEEISIIIGGLDVSGIFTQLLPCYSFLDSAYNSEPSTQCYSTTCVSRSISDARPEIKLSEIINKSVGLWTDYWKLTPEELAEINPREVQKQSYIFDLIILEERSMNMANAAVEIYGARFDPNLLPDEPEFASYAFDVFYPLIQLHKEYLLSPIFWKLKTKGKFIDGVGKIYLKWCNEAREYYIKYAIAMATVHEVILWEKQHGTRFAQWLKEIDNSPEVTRSKMYHDVIFFGGFFKSLQNLPITLSSILKNTEPSAEDYEYLKLVIPEVEKLSADVDRAHGTAIDHRGLVRFAKQLIIRQSENGSYVNVVNSANSNLAGRTHDRLDLGLTDPEKKIMHSGTVFKKREMWLDTSTLFIVLLDNYFLITEFFRKGNQDRFKLVERPIPIDYLNLEQRRGQDMPGSASTSTRESSFRENKQTVQTPIMAVRPHLISAATTMRTIYSSDKTLANSMDTNTTNTGSVEYSFKIRNTATSESFTFFTPTLQEYQSWINAFMECFKSNRSSSRAFDLHVLSTQFGYTEKEAPVNLPVAPEGSEIDVALKKYEAKLSRREEAEKELWVTPTTIFCSTDFEIDGEKFRLIATDDGILIRHESSYKTIFVKLIQMNCVRQMEINAKLGLLFVLDDKLLCYFSVSSILGAYYDPKKYAKSNHLVGVVIKDKVGYFKLADDFGNSKHLFYERKNKIVVLTPEFDRITRLLKYFKKYKEYRLPSSASGLLGSDVEDIVVFKKSFILCTSKGTILYHDTFNDDGIVLPNFLNDTTMLSYVKQGHSSSNLFKAAIESSSKKDMSKQKMAEYVKKDIATVKTKTKTCFQIANSKNFLLIYDEAVIKVNRQGEMEDWTKDILVLNFYCTGACFHEGYLILVGDNLIQIYKVTDSEVELARLIPVQIIKGKKVKLLNSSKTSNPVFVLSHPQIANRQLLLTCSPCNPKKNKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WV30</accession>
    <name>A0A8J2WV30_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="4">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BN860_11958g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA polymerase type-B-like family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="4"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WV30"/>
    <dbReference type="OrthoDB" id="273917at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071044">
        <property type="term" value="P:histone mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071042">
        <property type="term" value="P:nuclear polyadenylation-dependent mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071036">
        <property type="term" value="P:nuclear polyadenylation-dependent snoRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071037">
        <property type="term" value="P:nuclear polyadenylation-dependent snRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000006">
        <property type="entry name" value="non-canonical poly(A) RNA polymerase PAPD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000003">
        <property type="entry name" value="non-canonical poly(A) RNA polymerase PAPD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002058">
        <property type="entry name" value="PAP_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF15">
        <property type="entry name" value="INACTIVE NON-CANONICAL POLY(A) RNA POLYMERASE PROTEIN TRF4-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03828">
        <property type="entry name" value="PAP_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="16">
        <location>
            <begin position="152"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="PAP-associated" evidence="15">
        <location>
            <begin position="344"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="40"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="513"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CDF87677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="574" mass="65440" checksum="8A2BAF9CED9672A9" modified="2022-05-25" version="1">MRKSSLSRAQRNFQVFNQNDHVNKYESLRDIPVIDVSDVEKESPQAVVRGPVIEVDSETDQESGQESTTRVAAAVPDQEQEPEVEGLQNNDDFIALSDSSEDEELAETSQNGSKEQEGEEEQEREAGQSVNTDYPWILNHDHSKQKEIADWLTMEIRDFVAYISPSRQEIEVRNNTIRTLRKAVMQLWPDADLQVFGSYATDLYLPGSDIDCVVNSKSRDKETRSSLYSLASFLKQRKLATQVEVIAKARVPIIKFVEPKSQIHVDVSFERINGLEAAKLIRSWLEDTLGLRELVLIVKQFLHSRRLNNVHTGGLGGFSIICLVYSFLRLHPRIVTGEIDPQDNLGVLLIEFFELYGKNFGYDDVAVTVSDNRPGYLPKNEWRELQPARGTFSLAIQDPGDSSNNISRSSFNLRDIKKAFAGAFDLLTNRCFELEGATYKDRVGKSILGNVIKYRGKLRDFNDDRSLVANRAIIENENYHKRRSRIVHEEVFVDPSESESETLKEEQDIYHIEGPPKKKRQTKSVDELMGIGNEEEDDYNPLSRENEDATLKKITVDAQTKRDYWLNKGNSLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WV80</accession>
    <name>A0A8J2WV80_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein ARV</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_13432g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediator of sterol homeostasis involved in sterol uptake, trafficking and distribution into membranes.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Regulates also the sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Golgi apparatus membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ARV1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WV80"/>
    <dbReference type="OrthoDB" id="2192830at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097036">
        <property type="term" value="P:regulation of plasma membrane sterol distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007290">
        <property type="entry name" value="Arv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467">
        <property type="entry name" value="ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467:SF0">
        <property type="entry name" value="PROTEIN ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04161">
        <property type="entry name" value="Arv1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 10" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="251"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF87743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="290" mass="34129" checksum="A5C0C716BED09DFC" modified="2022-05-25" version="1">MICIECAEPVDSLYTIYSNKHIQLTDCPKCHKVVDHYVEFDNVLLFIDLLLLKPGAYRHLIYNSLDYKLSSNSDSNPKCSEHVTLTWLPTLFYRFRNWLIKYDKLNRVWLLLIAFEIYLTWVIEERSYAMNSGFLEGANSTRINPMLRKVFAWDPLHQYLYFAFYCLLDISIFHSLTRHCIVSWSKWGREVQNAKDVLSYAILLSYGAKIFPILMLIWPYDTVFSMNIIKCVANLYIVESLKIVTNLPYRWILQVFAFVLLVRWTIVKVATALLVAGGDWKLLKTFFETI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WVG5</accession>
    <name>A0A8J2WVG5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_16248g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_16248g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WVG5"/>
    <dbReference type="OrthoDB" id="5592879at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990758">
        <property type="term" value="P:mitotic sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033338">
        <property type="entry name" value="Spc105/Spc7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013253">
        <property type="entry name" value="Spc7_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28260">
        <property type="entry name" value="SPINDLE POLE BODY COMPONENT SPC105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28260:SF1">
        <property type="entry name" value="SPINDLE POLE BODY COMPONENT SPC105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08317">
        <property type="entry name" value="Spc7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00787">
        <property type="entry name" value="Spc7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spc7 kinetochore protein" evidence="3">
        <location>
            <begin position="230"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="61"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="386"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="444"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="213"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF87865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="695" mass="79858" checksum="2C652ED1061F190D" modified="2022-05-25" version="1">MAKGILKSSQNDSQEEVTRRHSFAGEDNDVPEGNNSTSRIHTLAQRRVSFAPDVTLHSFDFVPEVRDVRLPRRRSGAVAGGAQEEPANRVPGDESVDDRDDRDRQPPEEGVMEITEVFQPSHEMEMTEIFKRHDETMELTEIQRAEDSEPMELTEVQAHEVQAQDVLQRVHTPPPKRRKISDLSEDMELSLMKMSPIGLHSPQKPQERQAPRSLPPPSSSPSPPARRSSSPPLPPPPAPQERYSLRQFLDDAGVTFLIDTTWVETQDPVVFPLRTLSAPQFRTDQVYTPLYVDMPVLEMSAFVCRELLRRIEQSRRQFDDLEQQVSSSPPPLLLKEYFTSGPEMRQLMNQQLQLVKSFAKLEAKKAWYDWRIQHLKGLQNVLTENLALLQDEQTKLDADLQRARRINTRTAELLQSLRREVELVKGLPSQVYKKESRLSDKLRLEQLKQELGAHKIALNDAQQLQRQRQTILSEINSKTRECAQLKTRISSQLSHRKADASEYDMVKLRKKLDMLGVISGVHFRELRGSLLSIECTGFPVLTIDLSLCHSARQQACQVATEDPFYGHFLQYIIAQLPEDTDFLMELMEQLRMSIPSIGQYKLLKLLFPMRIAINQEKIFVELKDYDPHTNTCCFYKFSFEELVKVMLRESSSLSISVHVITNDDVSLAMLSNRFITKTRKILPWANANKIKMLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WXD8</accession>
    <name>A0A8J2WXD8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BN860_13872g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_13872g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNU66/SART1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WXD8"/>
    <dbReference type="OrthoDB" id="5583at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000481">
        <property type="term" value="P:maturation of 5S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045347">
        <property type="entry name" value="HIND"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005011">
        <property type="entry name" value="SNU66/SART1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14152">
        <property type="entry name" value="SQUAMOUS CELL CARCINOMA ANTIGEN RECOGNISED BY CYTOTOXIC T LYMPHOCYTES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14152:SF5">
        <property type="entry name" value="U4_U6.U5 TRI-SNRNP-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19252">
        <property type="entry name" value="HIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03343">
        <property type="entry name" value="SART-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="180"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="351"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="393"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="201"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF88592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="476" mass="53952" checksum="24CA83AEE0502623" modified="2022-05-25" version="1">MPAEVCMSLEETNAIRKQLGLKLIELEETGSKAENVLGTSGKKDDAFFMDKEKIGQLRKRLARLNHNISGEEIQPLANDAPDDDWLSRVGKKRQRQVTVVHEEEPEEDLPLLQLSHSVQELNSGKDVILTLKESSLKSDDEPVLEDAHLTESKRDSKNSRLKKLNKERKNNKVNLKVSSLDIEQDEDSDDDSPKSVLMVGGQTNSIETGQRSETPVAEARRDQIKVQFEDVANESYEGDFQPVKIKKRKKKDVKSRTKSPSDIRPVQLVDEDLGLEEDLLVTTLSRPLLKNNTHDPDAIISQIRREHFEKQERTSNIAHLNKNTNLVFNENMGFLDSLKGNTVTMEMPRDVSQQPDNAADEHIGQPIRTPQPEVESPDFSKGLASALQFLREREVPTSSSAEDEQTSGNDKRSQGYNPEINLIYRDKKGNQLTTKEAYKKLSQKFHGTKSNKKKRDKFDARVQARARLQNNDVELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X234</accession>
    <name>A0A8J2X234_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S07-00232g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_00232g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X234"/>
    <dbReference type="OrthoDB" id="2238745at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF174">
        <property type="entry name" value="ARRESTIN-RELATED TRAFFICKING ADAPTER 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="331"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="636"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="939"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="575"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="659"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="688"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="702"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="728"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="947"/>
            <end position="959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF90405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="990" mass="109756" checksum="DC17DA322784512B" modified="2022-05-25" version="1">MEELEAVRSPIFPIEKDVDVSSDAEPLSQTSSIQVFIRLAEPVVFLQGFNSSQQSEVPPSMLRGSLILRVLKPSKLKNVSLSFKGYSRTEWPEGIPPKRQDFVEICDIVNHVWPFYQSDEHNGALGRTDDNSYLLRQSGASVFKSLETVRNRRKSASSHTPRSSLVDGGVPDSARRRSNSSAATRESAGAGRSLSPMSLLRRAASPAPKHDRSSNLISDLLSSTFSNTGDTFAGGSHSTQNGGDSDTSGSGDHFVFQPGEYIYTFEQAISPSYPESIKSDFGFVEYHLFASIERVGAFKSNITARLPVTLVRTQADSSVEETEPIAISRDWEDQLHYDIVIASKDIILDAFLPIAFHFAPLDKVTLHRIRIYLTETMEYYCRGKKVHRMEPTRKFLLAEHNGPNLSGTENGPTKAKNMGNLLLEQSSGDLVNKDYEYQIFVPSVVSERHVLHPDTAFDKIKSSHWIKLCLRLSKMVDGKRKHYEISIDSPIHVLNKLCSHANTLLPSYDSHIFTNEQAVNACPKFGNTADVNIYHNSNIFFPKEILFSPLLSPEVHSMDFKLAHNVRTSSQKPVRTFDRRSSKTEEDPTFASPRLRSNIYQPETIRRELASPQAIPLSPVSSPLLRSISPWIAPPGFDFDNDEPRNDNDLPINPPSYDDVMRDDTLEDTQKKEISTKVPELPKITLSRSHEGNNEEKKISLTGGTRRSLENSFQGNDKEAEEDGDITSGFSFQGVSRTSPNLPGAVLRSPMMKPININSDVKTPSKSPLGTSLPATVRNDNPIYSGLSEMLDCGLDDFAVKRSVTNESNSSSVGMPSTIDGMAALGNRNNMMPLLHQSESRPNRSLDDLANQSRDSLANYAEPPVDSSVDITALYDRNSTPWHPLQMSLGEALSPVVSPSYSSNVAERNHVIQDFKEALHHNCSSQESAADSVSTLKQNGDVFGSRESPDELHSSDFRFHPSSKRSSAEKQVINEVGREATFSSLQSGFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X528</accession>
    <name>A0A8J2X528_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_09164g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X528"/>
    <dbReference type="OrthoDB" id="347657at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000196">
        <property type="term" value="P:cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05581">
        <property type="entry name" value="STKc_PDK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000534">
        <property type="entry name" value="Serine/threonine-protein kinase PKH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039046">
        <property type="entry name" value="PDPK1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057614">
        <property type="entry name" value="PH_PKH3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF405">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PKH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25347">
        <property type="entry name" value="PH_PKH3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="11"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="290"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="356"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="516"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="359"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="379"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="542"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF87559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="843" mass="92561" checksum="E02B9EAE062A03DD" modified="2022-05-25" version="1">MSSLKRSPHDFVFKQELGNGSYSRVYKAVDKGEPQRVYAVKVCSKRHIINENKVKYVTIEKNTLNLLARAHHPGIVKLHYTFHDEENLYFVLDFAAGGELLALLHRVGRFSEIWARHFIAQLIDTTEFIHSQGVIHRDLKPENVLLHRDGRLMVTDFGTATNVNRDNGDKGASFVGTAEYVSPELLLYNQCEFASDVWALGCMLYQFVQGSPPFRGENELQTFEKIVSLEYPWANNTTGGVVALVRRILTLDPSDRPTLSAIKQDKWFESVDWRNRELLWRGIWQVQPQRQQQLQNTDMQTRNSPVAKQKRRKPAKVANTTSSIVEWRKQLGIANEYLTNPGGPNASSINTRKAIKPAGNLNGSTNNMNNGTNGRPAVRSNSMSKVPALSQVQSQAELQPRLKSPSPKSSGNIPKTPMALKPQLPPGSNTKVLTTPAPLNRVTIQQQLTQPQQSLLVPKASSTPNFSSRVPSPVGKTKLMAPGSIPKAPSPIGKAPALAPALAPATYVQAPTPAQPPILAPFNKPSELRRAPAGSATSPPESAAHTSATVSSVPIPPTIAQAVVRKPQSPAPRTAPPKLSVDTGQRPSVMTNGSTAVIATTNSGSKTVDPTIIKQDSVNIFEIPFNGSGPDMSLQSYKRVDNELITQLVGEHKSELRPSGAMLSLLTLYKNGTLQYKQKQSVHQMANIRDTSLSMYDYEFYDKQARGFLILEKYHCKLWFISLPRGNALQQSSQGAIVNADENWVDCIFRARQLVEDQKLADRLNNVEVSSPRALSPTIPVTPIYAKARTISGGSFTPQKKYAIPSNIVVSSSRKQVLHAINRNGGGDASLGASAAFKSLQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X574</accession>
    <name>A0A8J2X574_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_11012g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_11012g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS72/YL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X574"/>
    <dbReference type="OrthoDB" id="49520at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013272">
        <property type="entry name" value="Vps72/YL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046757">
        <property type="entry name" value="YL1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13275:SF4">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 72 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13275">
        <property type="entry name" value="YL-1 PROTEIN TRANSCRIPTION FACTOR-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05764">
        <property type="entry name" value="YL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08265">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00993">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vps72/YL1 C-terminal" evidence="3">
        <location>
            <begin position="699"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="17"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="298"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="450"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="120"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="309"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="342"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="387"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="400"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="454"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="479"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF87637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="773" mass="88876" checksum="EB275435695B857C" modified="2022-05-25" version="1">MNDDDLELHEEEFLMTTRKRRSNAGNKMRKLLEQELEDMHSRTENLEQDEVDLLFQEDAEDEEFEAGDESDTELTEKSNDVERKGEVDEMFSESEEESEDQNEDDEEGEKVLQRQEKLESRKRKKKQTPAVIKRKRVNPETDAAKEEQKSKKKAHYEQIKAENLLISNRRTSQRSSVVANKMKVYEKLSKAEKKRKVIQDRIRKHREAQKEEILTQEDRIRIALETEKFNILSLDKYKEQEVSKKQSRIALQQRQKLKFIPGETVIRQLSCSWAVTPAMELEDLKYWEEQVKRREKKKKKYTRRSYKKHNQDSKNEVQESKVKEEPCISVPTIEQSPSKASADFRETKEEFSSKVPTREKSPNYSESHIDIKETPEFTPSAVSDNSLRSKTESDAKIGESDTTPLTGNNNEIKASSHPENFKTQSLLVTNRNEEIVAGNKHAERLLPPVEIEAEDVHQNPKTENEEEKLSGKHSVIPDNEYKNTSESTRDNSPAIFLPEEQTSSPQLSNNKTEALENQSLISSLIVSKQVTFAEEPQVTLINSSTGLYGDSPSTVSTTTKKYEREAPDLVDDFGSEEETVFQGPEQLVAKNFVTLYSFPNETYHRDVRKELFGVNWESGLSHRSSDVETICKLTMPEEKDDFDTASLTPNLSFLNKFPAFGEYDKKVVHDTDASVNKEFEIEIKTSPPTGVYLPNGLRKKCLISSKECQYFDPKNGVPYSDVETYKIIQELQNPIGKGTEEEPKPRFQWFGFSNGGIYLDISEKPANGVPDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X583</accession>
    <name>A0A8J2X583_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">BN860_11364g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_11364g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>myo-inositol(out) + H(+)(out) = myo-inositol(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:60364"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X583"/>
    <dbReference type="OrthoDB" id="6339427at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005366">
        <property type="term" value="F:myo-inositol:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904679">
        <property type="term" value="P:myo-inositol import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000073">
        <property type="entry name" value="MFS myo-inositol transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="399"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="441"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="485"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="511"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="87"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
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    </evidence>
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            <dbReference type="RuleBase" id="RU003346"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CDF87652.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="581" mass="63268" checksum="46A87CCE2720AEE1" modified="2022-05-25" version="1">MDFSWGRSTSPRQSVELSSPEDNTVLQPESLEGEKKEDGSHLTRTLTPSSETKFKPEDHEFTDEREDSSSELVEGFDSGVSGFIIILTFVSSISGFMFGYDTGYISSALTSIKQDLSHKTLSYGDKEILTSATSLGALISSCFAGVAADIFGRKPSIMGSNILFLVGIILQICADKYWQMAAGRLIMGFGVGIGSLVAPLFIGEIAPKTIRGRLTVINSLWLTGGQLIAYAVGAGLTHVHNGWRILIGLALIPVALQFSFFFFLPDTPRFYVMKGNTDAARRVLRRSYRNAPDYLIDAKVKELCDMNNAVEGKNVLDKVWKMTKELHVNPANFRALIIACGLQGIQQFTGWNSLMYFSGTIFETVGISNSAAVSIIVSGTNFVFTIIAFFAIDKIGRRPILLVGIFGMMVALIVCSIAFHFLGVKFNGADAVVLHTGFTAWGIVIIVFIVVFAAFYALGIGTVPWQQSELFPQNVRGVGTSYATATNWAGSLVIAATFLTMLQNITPTGTFALFAALAAVSFIFVFFLYPELSGLELEEVQSVLKDGFNIKKSKALAKKRKKQVAAAKKKVSSSEIIEEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5B5</accession>
    <name>A0A8J2X5B5_ZYGB2</name>
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        <submittedName>
            <fullName evidence="7">BN860_12882g1_1</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="ORF">BN860_12882g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NGG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Histone_AcTrfase_su3"/>
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        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10198">
        <property type="entry name" value="Ada3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="512"/>
            <end position="521"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00005330"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="CDF87719.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="609" mass="68773" checksum="C20439B26E01BA61" modified="2022-05-25" version="1">MAKRGKTPKDVGGEEAVEYEPPSKLLSSILSTLDMTFERDTGMLNGKYVRVVPSREILSELKILLGKLHVSLDSIAKGDQEVISRIEKLKHGTGAVKDEQENDGSQPTLPQRRKRPPLTDIGEEDEVTLAAPEIKRPRTEVEKMENDPSVKNPKSEFVVSQTLPKAAASLGLFNEEGLESTGEDFLKRKYQVASYPTNDLKEYLPGEIPDHNYSHPKTTNQIQYTTFLASVENFFRDFNDDDLKFLKSKYIIPINLDVNKTYDPEVTPYVIPKLGPLYSEVWAKADDSQNIANTSPPPVSDPSSILPKKSAAEINDSMLDTEEVSCGPLVSRLLSAILKDEVDGEDDNKVVVKEEGSQSTVPQDELESREETPMSMVHTPVSEMDEKSVRGSTTSTLPNPQLWKVNSINLDYPTFEERLKRELRYVGIYMNMPRDEGANGDDPDWLTGREDDEISAEIRELQTSLKHVRERNQKRKAALVPLVERQLSWQEYSSILEDLDKQLDQAYIKRIRVPKKRKKNHSSGSANSVNQGTASQVAQQKAANLSLKSLLDKRQRWIKKIGPLFEKPEIMKRIPKESVFKDLDQEEEEEETDVFGQNGNGKEDELTET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5F6</accession>
    <name>A0A8J2X5F6_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">T-complex protein 1 subunit zeta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">CCT-zeta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_13300g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5F6"/>
    <dbReference type="OrthoDB" id="10052040at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03342">
        <property type="entry name" value="TCP1_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000004">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012722">
        <property type="entry name" value="Chap_CCT_zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02347">
        <property type="entry name" value="chap_CCT_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF87737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="546" mass="60125" checksum="F0EC5A3D18E467BE" modified="2022-05-25" version="1">MSLQLLNPKAESLRREAALRVNVTSSEGLQSVLETNLGPKGTLKMLVDGAGNIKLTKDGKVLLTEMQIQSPTAVLIARAAAAQDEITGDGTTSVVCLVGELMKQAYRYIQEGVHPRVITDGFDLARKEALSFLDSYKIARDVEEVDREFLLQVARSSLSTKVGLELTEVLTPIVTDAVLNVWNKEKDSLDLYMVELMQMQHLSPKDTTFVKGLVLDHGGRHPDMPQRVENAYVLILNVSLEYEKTEVNSGFFYSSAEQRDKLASSERNFVDSKLKKIIDLKNEVCGLNDNKGFVIINQKGIDPMCLDVLARHNILALRRAKRRNMERLQLVTGGEAQNSVDDLTPSVLGYSELVYQETVGEEKFTYVTGNKNPKSCTILIKGSTHYALNQTKDAVRDGMRAVANVIKDKTVVPGAGAYYLAASRHLKTCNLNKLGAKGKMKTGVEAFAEALLIIPKTLVKNSGFDALDVLALCEDELKDAEETSQRRFVGVDLKLGDSCDPTIEGIWDSFRVIRNAINGSSGIASNLLLCDELLRAGRSTLKEGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5N2</accession>
    <name>A0A8J2X5N2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_19328g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_19328g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5N2"/>
    <dbReference type="OrthoDB" id="10261782at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038921">
        <property type="entry name" value="YOR389W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35204:SF1">
        <property type="entry name" value="ENTEROTOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35204">
        <property type="entry name" value="YALI0A21131P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="627" mass="70868" checksum="B1EED1B894D2CCB2" modified="2022-05-25" version="1">MRKRFALSVALPTALLLVFATWIVLFQSRSTSDLGLPKFIPLRESKEKITWRNFQDKVRPIDLENSTAIFNSIYAALRQSGSDLHPVGVTYFPAIIPKGTLLFHAGKGEMPEGLEWLAMDHEFSLNFGSRRQAYGRRSLSRRGKGPGGPGGPGKPGGPPGSGKQDQSSDQPKKNFDRSGSMTMMTFRATRDLNRFLYLDGASASKSTTGEMDTQKMISDLVGSQSNSTSDGDNGKRVMSERLYAERICSWGKKYGLEGIIRVEVGFELVLCDFLDGSTELVSNNSYPLVNDMLGLPPPTKITAENGWPLDANGTLIEDQLTEEQRAILKKEDDWETRMRKFDTMDSFGQLLAGEIHDKGDRRIHLDYRHMVTGLNRTYINPDPNNRRLLNDDISLEVELGMLHDLENSLKGGFDSSESTDWQQVLDEIVDKFSPMLKGMERVLDAEDKSVNIVAENITRYTTSFVRRFIEKQREGSKLDSGRDFAIYQYSRPLKDLATDSDYLIWSAVVRVVTEVVDTIYSLHETLWPIVLSEFQGDVVDDAYSRVTDSQQRLKGLIKALNWIPLNFECEKKCDWDEVCYTPSWGPSPLGWTLPDSTDEHFGMHFDSKRQRQVIDSELRCVKIDSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5P0</accession>
    <name>A0A8J2X5P0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Altered inheritance of mitochondria protein 24, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_17590g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIM24 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5P0"/>
    <dbReference type="OrthoDB" id="5295771at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.160.10">
        <property type="entry name" value="Mitochondrial biogenesis AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036983">
        <property type="entry name" value="AIM24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016031">
        <property type="entry name" value="Trp_RNA-bd_attenuator-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01987">
        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51219">
        <property type="entry name" value="TRAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="272" mass="30011" checksum="C7DCEF21EBAEDDD0" modified="2022-05-25" version="1">MITQVARRSLLRSFTLRRYGSGSIKISDFSTTKKGTGAGPFVELPEFKQLQDSLLIKLPSSCTVYGNLDKISAITSDGLENGKPFLAQDYDSTRGLSMVTTGDHAANVLVASYTPLSNVVVLEMDNYEEGFCVPNFSQEALCFSGDLHLDNTGHLRGHGVVALAGQGPVYQMILKKGESIVVAVESLLAHDKNIQLQLTKLNSSFSIPQRINDWLLKNASDVYEKLLIQWIRLFSKDKIYYEIQGPGIVLLQTNFVPGSKTYSRAELLKVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6J1</accession>
    <name>A0A8J2X6J1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP-dependent rRNA helicase RRP3</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ATP-dependent rRNA helicase rrp3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">BN860_17942g</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent rRNA helicase required for pre-ribosomal RNA processing. Involved in the maturation of the 35S-pre-rRNA and to its cleavage to mature 18S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with the SSU processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family. DDX47/RRP3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6J1"/>
    <dbReference type="OrthoDB" id="10261904at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17954">
        <property type="entry name" value="DEADc_DDX47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000626">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044765">
        <property type="entry name" value="DDX47/Rrp3_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF24">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 18" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="22">
        <location>
            <begin position="76"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="20">
        <location>
            <begin position="107"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="21">
        <location>
            <begin position="302"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="33"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="473"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="17">
        <location>
            <begin position="76"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CDF87941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="496" mass="55176" checksum="55C08AD8ACC9DE19" modified="2022-05-25" version="1">MREGKILKKDKNCSKEKHKELTSLAAKIKAKALENQRKLKSPNESTSEDSVKHDEKENVKDGQAQDGQKDEENTLESFNDLKLVPELIEACKNLKFDKPTPIQASAIPPALEGRDIIGLAQTGSGKTAAFAIPILNKLWEDQLPYYACVLAPTRELAQQTKETFDSLGALMGVRTTCIVGGMNMMDQARELMRKPHIIIATPGRLMDHLENTKGFSLRKLKFLVMDEADRLLDMEFGPVLDKILKVIPTQGRTTYLFSATMTSKIDKLQRASLTDPVKCAVSNKYQTVDTLVQTLMVVPSGLKNTYLVYLLNENIGKTVIVFTRTKANAERLSALCNLLEFSACALHGDLNQNQRTGALDLFKAGRRSILVATDVAARGLDIPSVDMVVNYDIPVDSKSYIHRVGRTARAGRSGKSISLVSQYDLELILRIEEVLGKKLPKESADKGIVLSLRNSVDKANGEVIMELNRRNKERTARGGNRRTRMNAKANMDKEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6L9</accession>
    <name>A0A8J2X6L9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Signal peptidase complex catalytic subunit SEC11</fullName>
            <ecNumber evidence="4 14">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_19020g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Specifically cleaves N-terminal signal peptides that contain a hydrophobic alpha-helix (h-region) shorter than 18-20 amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the signal peptidase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the peptidase S26B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="4 14"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6L9"/>
    <dbReference type="OrthoDB" id="10257561at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009003">
        <property type="term" value="F:signal peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06462">
        <property type="entry name" value="Peptidase_S24_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019756">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015927">
        <property type="entry name" value="Peptidase_S24_S26A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02228">
        <property type="entry name" value="sigpep_I_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00717">
        <property type="entry name" value="Peptidase_S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00728">
        <property type="entry name" value="SIGNALPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00501">
        <property type="entry name" value="SPASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Peptidase S24/S26A/S26B/S26C" evidence="15">
        <location>
            <begin position="30"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF87986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="167" mass="18911" checksum="B615BF39B03FF228" modified="2022-05-25" version="1">MNLRLELTRFLKLCFVLSSAFMFWKGLSIVTNSHSPIVVVLSGSMEPAFQRGDVLFLYNRNTINKVGDVVIYEVEDKSIPIVHRVLREHHNDKKKQLLLTKGDNNAGDDIPLYGRKKNYLHKSKDIVGTVKGYVPQLGYVTIWISENKYAKVALMGFLGLSALLSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6P9</accession>
    <name>A0A8J2X6P9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_00914g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_00914g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10684615at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025928">
        <property type="entry name" value="Flocculin_t3_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13928">
        <property type="entry name" value="Flocculin_t3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035251942" evidence="7">
        <location>
            <begin position="20"/>
            <end position="1790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="224"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="290"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="507"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="548"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="743"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="844"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="888"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1066"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1143"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1312"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1434"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1641"/>
            <end position="1685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="224"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="250"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
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        <location>
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        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
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        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
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    </feature>
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        <location>
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    </feature>
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        <location>
            <begin position="1154"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1312"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1346"/>
            <end position="1356"/>
        </location>
    </feature>
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        <location>
            <begin position="1357"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1434"/>
            <end position="1466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1467"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1641"/>
            <end position="1661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1662"/>
            <end position="1680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF88040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1790" mass="184907" checksum="E550346157C336AA" modified="2022-05-25" version="1">MKFPRLLLLLTVVLDPAIAQSSYFSNTSAAYTSPSHSSSAKIAGSGAYISESDYSNSFIFTGTSNAAASSSVIYRESGRVSASSGLLQVPNTIQKTTSSSASSLEPTLSSSDISNVPASSSLRFEHLSGSSATVSDNSSHISTRVSSDSFAERSISATVSSSADLSKNVTTSIAYSTVDGTLTDATESWRTITLSTEVCSHDSCTRSISTTTSYVSDIGSHSSSQIWSTESTSSPYRSSTINAQTSPTKLNNSTDLSSSSVSSEDSNNSTPTLILSSSYVSSLEVESTVIPGSTSEVSNTSGTTQSFRDTLSSTSFSSTTSTSSTTLSVLSEASSKISSTISGSSSSSEITLLNSTISGSSASFLSTWPITLASSTSIASVRGGSSYPSTSTLSDHVNISATGGSLSSRASDTGDTVLSSKNSFPMNSTLTSIAPKSQLSSTSDYIQAVNTSLTENPRVTGSSNGPRDIYSTINATSFSFAESWQTITLSTEVCSHDVCTKSFVTTTTKRNASSTTPENSVETSEVSHSEVSHGVASTSQTSTLALTKYPSTISPSPSHDSSTSNPSSSLTASLSSSFEANFSASLSASSIYFKITRTVSHVSTVTTTIDGTSTVYTTWCPLTLTSLASSELSTKSPTSTENSISLTDEEYFSGTSLEVQSPPLSSKYGYSSSPVSTTTDVLNSSSSMPVDVTSATPIPSSAYSPETTRLLSRITTVTTTINGTSTAYTTWCPLTESYSTSSTSPKQTVSTFGPSQSVNSVSSSHAAHSSVIGNTTQQNSESTGTFSTINDITTALTESWQVITLTTEVCSHTSCTRSILTTTKNKGNPGSAISSSSAATISEASVESAGPATSSSHKAIHQPSSSSTIFNSLTPSIITSSSIFSTSSSTTEASSTHTTGSSTKTTSKATSQTEDNPRSLISTLSTVSSDAIEYISTSQQSTEQTLSYVTESAHKAGTVSSDSKLSSTISTVVTTINGTSTVYTTWCPLTSDFETPSQSITGASSSPIEISSHTGDIPPTVSSASSTTISRPVSVPSSIVSTVVTTINGTSTVYTTWCPLTYESATPSPSVTGTSSNSDEISSHTEGTSEIASSISSETISQPVFNPSSVISTVVTTINGTSTVYTTWCPLSSEIKASSYVKSGSTSINTNPGPSTISSNIESTSPSTSPGPSTISSRIESTSKTLPSVISTITSKNVPGHTGIISTVVTLISGTSTSYTTWCPLSSTSNVYYPSLESESSATRISDSGNVESFTSFTPQVEVVTSIVHSWETKTLSTEVCSNRECSKFLVTTSNPITITRLSTAYYTSGTSDLNTVSPSSTRENSKSEGTISSYSTSFSKAKQQSSPTHSESISSPDTSPQPHNSNSFTASTVGSTSTTPLKTQEMRTISTIITTISGITTSYTTWCPLSDESSLHSFSTPNSSRTMETVVKTHTTQSSGETVSSISSKTTKPSVISETGQSVTLSEVGSTSSASVAGSSHTTPPTATLSYISSSAASTSIITSWKTVTISTEVCVDNACSQSAYVTTTPHAITLYSTIVSTNGWGNSSSQYARNSSSYISVATRPSASLNVSSLTVGTTRATANVSTIESTVNGTITTYTTWCPFSSINKTPAEVSGSLSTYSLTSTTTSVICPSCRAGNSNTSRTVPSTKTPAPSANGSTDDSSIVSSSSTSIDASSRTAETVFGSEVTRTMSSLGTTTISVQSCSEENCTTSRSSITSAALTTSPIEDYPSSTKYPSINKVASPSATSSATGHDTSSNSLQVFSAGVSKLVQSKALTIFGILVCLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6R0</accession>
    <name>A0A8J2X6R0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_12970g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_12970g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6R0"/>
    <dbReference type="OrthoDB" id="10256176at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="450"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="457"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="518"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF88555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="794" mass="89312" checksum="264C8FE944C68741" modified="2022-05-25" version="1">MEGIKVSRGILQSGNSSRSIPHSDGGSTHDSIDKNNRDGDEVDKEATKEEITKLQEEVASMKGDNLQEFMQAKENTSKERDKTSTSMSTISPKPTRRRPTKNLDLHQAETQEDFYGTNRKSASPMTKSARVHSHPFGRETDGAIDGWSQLTPTSSLHGNTSKVAATHNRRRTWDWNNLPRAGSSILAKYHSFNIISLPIDGEIKQILYSPSYNENFKAMNIFLSFNMDSKSKDAQLQSKKRLNSFGTYIGHYLRSRLYAYQCFPFYQQEPPVDRTYASYDSTKDYSEVETIISLWYMQGQRCISQSNSVFFSAEVVQHLLQRKVNSRNQAVQQTAIYVPQTGATLMSAKPNTTETTEDIDILLLRPHMEPRLGWQLAYDESNLKVADYPLDTSPWMMEESDAKYILGSQNSKISSSVVNIVNDEKDVVRISNENAGRYFLDCMGRRTGSISEDAGSPVTSAQMSASPSFKEDTPLIGSGQGSPPNTNEPSRKKVASKLGKKTGLAHLFKRKHTPVASVPSSPQDKSSQMVSDNQGNSIENAWLESYFSRYLSNYKKIDLPTQFLLPKEAQDTPKVRSPSGNQELPQSKKAFLYSKESLQLKLPFADDLIPAIFCPRIWMSLAFSKWRSLLREMYRVLLPGGYAMAAVTDIGVSNSFTQTTEGNMQEFPTTQERDRVLDAIAVAAIEKGLHIYPTQHLAQAFKDVGFTNLKYSVLSLKTGDCSTEMGCLYEIYSEITWDLLFRKHLPDPTKPPKGTEPTTLFRRFIREHMGKVDDNAGSLRTLYLVAQKPRKSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8B5</accession>
    <name>A0A8J2X8B5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S05-03840g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_03840g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF89878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8B5"/>
    <dbReference type="OrthoDB" id="531008at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006828">
        <property type="entry name" value="ASC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037256">
        <property type="entry name" value="ASC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF87">
        <property type="entry name" value="SNF1 PROTEIN KINASE SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04739">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01010">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160219">
        <property type="entry name" value="AMPKBI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Association with the SNF1 complex (ASC)" evidence="4">
        <location>
            <begin position="649"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="139"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="261"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="373"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="519"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="182"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="229"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="273"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="292"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="373"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="388"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="557"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF89878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="776" mass="85687" checksum="0D618AE6A0E09788" modified="2022-05-25" version="1">MGNSPSVYQSRGERGASSTIQDDTAIRATYPHTRNLMEMDNGRQNSITSQLFPSRKGRHVPGGLGNIMRKTQNAVPHPPSLFKKNYSLEGANAGVEASRESAGGAARTSSAESVRANMAGLSLKNSNETAGPMANPYFMSPGPATPTVQPTKEEATPIDDVDVQNLPGHRRSVVALKKSLQESEQSPSVSDSPSITVTRSNSIDIKPPKGAGVRPHQSHTGNRRNSVDTDFDSSDADNMSDDGYLQSKDIVLNQSVVHSMLRRDMKRKRGKEVTNSSNTNDLTAGAAPSKESTVEPSEHRATNQRTLDSQSSGERRGFSYESIESGHLPEIKKLKPISHDILLPHAADENPDDPHHIEPQRHFYSMDSFEKGSVEHREVEDETRKSSESSSASSSGASQPSEDVQVIIKWRDPIPEGASNKVSMVSEDIASTLSFEKQKQCGARNVFTMKFDPKSKEWYVPDLMLPAGIYRLQFLVNGEIRHSNYLPTATDSVGNFVNWFEVVPGYQEVEPYREEVEIDSKPLENEDISPASPGNHGQQSLVQPVPGKPERPPLAPMQSSSNRVLRSNTPHSDYTGISRSSSALRKSPLMQYTSSSLDLVTALQPKKYRYSSEIPELFKTPPATETNDENNDMTVGNMENPFPPSYGWPHYQKAGLNNKVVDCNQDQLFTHLQQGGALDAETAEQLFLDKYPVPDLPVYLNSYYLNKILSEFQKNNYNSNGSSGINHIIPHVNLNHLLTSSIRDEMISVGCTTRYEGKFITQVIYSPCYYEHGGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8L1</accession>
    <name>A0A8J2X8L1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Malic enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_05886g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="7">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF89969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8L1"/>
    <dbReference type="OrthoDB" id="5365701at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05312">
        <property type="entry name" value="NAD_bind_1_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10380:FF:000001">
        <property type="entry name" value="NAD-dependent malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015884">
        <property type="entry name" value="Malic_enzyme_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010052">
        <property type="entry name" value="PRK13529.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406:SF34">
        <property type="entry name" value="NAD-DEPENDENT MALIC ENZYME, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00331">
        <property type="entry name" value="MALIC_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="10">
        <location>
            <begin position="138"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="9">
        <location>
            <begin position="329"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="513"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF89969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="642" mass="71542" checksum="59AD6BB209CF5FFA" modified="2022-05-25" version="1">MIRVTKQAANYSSLTRPLQATTTRDTTFNQKAHDGCGQVHKVPVGNRARQMLEDGKPRLTRLSVDGPIECFLDSFQLLNSPLFNKGSAFTQEERAAFNLDALLPPQVNTLDEQVERAYKQLCYLKTPLAKNDFMTSMRVQNKVLFFALVSRHIRELVPIIYTPTEGDAIAAYSHRFRRPEGIFLDITEPDSVERRVSVYGSDKDVDYIVVTDSEGILGIGDQGIGGIRIAISKLALMTLCAGIHPGRVMPVCLDVGTNNKKLARDELYMGNRFSRVRGKQYDDFVDKFIKAIKKRFPNAVLHFEDFGVTNARRLLDTYRGELPCFNDDIQGTGAVVMASLLAALKHTHRELKDTVVLVHGAGSAGLGIADQIVNHMVTRGLTLEEARSKIYLMDRRGLITTDISEYTSPQQQVYEKNGDDWADVNTTSLLEVVSRVKPTCLVGCSTQAGAFNKSIVQEMHKYNKRPIIFPLSNPTRLHEAVPEDLLHWTNYDALVATGSPFPPVDGYRISENNNCFSFPGIGLGAVLSRANVITNTMISAAVDQLADLSPLEEGNSKPGLLPPLEVIQDTSSKVATAVILQALKEGTARIEEEDMPDQPGTKVQVPRDFDKCLKWVTNQMWKPIYRPMVKVEHDPEIHSHQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8L9</accession>
    <name>A0A8J2X8L9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_16534g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_16534g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8L9"/>
    <dbReference type="OrthoDB" id="4065690at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031459">
        <property type="entry name" value="Coa2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40020">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40020:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17051">
        <property type="entry name" value="COA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035268260" evidence="2">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="65" mass="7219" checksum="1D230FA59E1E0423" modified="2022-05-25" version="1">MRAATRNNVTNRLFMSTFLVAFSSVALTSVFPCPAHSLDSDSPVKELPQKKSKQQTLAQELHSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8R6</accession>
    <name>A0A8J2X8R6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_19042g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_19042g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8R6"/>
    <dbReference type="OrthoDB" id="187617at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.440">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002713">
        <property type="entry name" value="FF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036517">
        <property type="entry name" value="FF_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039726">
        <property type="entry name" value="Prp40-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11864">
        <property type="entry name" value="PRE-MRNA-PROCESSING PROTEIN PRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11864:SF0">
        <property type="entry name" value="PRP40 PRE-MRNA PROCESSING FACTOR 40 HOMOLOG A (YEAST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01846">
        <property type="entry name" value="FF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00441">
        <property type="entry name" value="FF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81698">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51676">
        <property type="entry name" value="FF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="1">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="1">
        <location>
            <begin position="47"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="2">
        <location>
            <begin position="119"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="2">
        <location>
            <begin position="188"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="567" mass="67700" checksum="1B6F43E8C1564419" modified="2022-05-25" version="1">MSSDSLWKEATDGNGRVYYYHAKTGESRWEKPNELLSEQDLALAKFGWKSSRTTNGKLYYYNERTKESRWELPDLQKLEELQERKRERVESEPVEEGTKYNTEKYSNSSQILCVPKKPKDEAEKDFIEMLKDNQVDSTWSFSRIISELGSRDPRYWIVKDDPLYRQEIFEKYLTNRSEDQLLKEHMETNKFNEAFWKMLESKPQIHYYTRWPTVKRLIADEPIYKHSVVKESVKKRRFLEYVGRLRDKYNESQQQIKAKALQELQDYLENIISSTDYNNNSIPIIKWQTLLNSYLFDNNKRYMANKHFAILTHEDVLTEYLKIVGEVEKRMEKDLATLQKYNYTKDRKARDNFKHLLRSTNIEIRANNKWQDIYPLIKSDDGFLQMLGTGGSSPLDLFMDIVEEKRITLAAQRSIAQNLLIDKDFQWNEKDDSINRKAIKALLIVEKDFQSVDSRDIDLIIDQLMELRIERIKKQKELEERAFEERKHFYKLMLQRIYTNGRSKPDSFEVALKDIQHTREFIELPSDEIRRHLFDMFKPDVGPARGKPFAVSNVKKRQLTPNVDLDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X995</accession>
    <name>A0A8J2X995_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_10242g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_10242g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X995"/>
    <dbReference type="OrthoDB" id="66726at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16616">
        <property type="entry name" value="mRING-HC-C4C4_Asi1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF88436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="627" mass="71251" checksum="A9774364819DD4EC" modified="2022-05-25" version="1">MNSSDTGASSIVDSFAYLNQTARYLVPNSVIFSSLINFPYRLGLSFVDAVRIQYQQAVEADSLMLSLTCMKEIVNYFLSSYAIASFITALILNRFVIMASLRSTANRVTLPLWSRVCLHLSAIMPLLYTVAQVLCQYGILQWFAPLDLAPFLSRTFAAYAWSNCVETFVATTTNTLPLEESDYSIFELSVQFYFLNRMKETSGPSSDYLPDCMMAALGRILIHVVEMFSCRKYRLIGSTVLNCGGIIHLGYKVKREGFESLPALTRYRNFPKIFSLFLIIMSVSTYSLAYLVRKDPFGSGGLDHQELQFYSFMHSWWTHLNCTGEEEFSYVVVKLALMLCMGHESMSKGVFREFPHVNVPSSIHHSYVISGYLNGLPTIPEDMDDKSHGGSVTTNAPGIVNRFRISLTMIKAFFAFFRKSKLHHPRLERSNKPKDCNRYVTERNYAKFLFKPKTNDDDKDSENNQYLLPDEDPSADYLPDPSDELETDTEWVSDDEDIGTILAPDNVKNLQEDISWHISMWSILKSTVQRGTRMTRKQYASLEPQKILTEVVLEKASAQENNMSDEDDIDPSFACVVCKTNPRNVVLWPCKCFALCESCRVSLGLRGFNSCVCCRREVHGYSKLHTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9B3</accession>
    <name>A0A8J2X9B3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BN860_11276g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_11276g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X9B3"/>
    <dbReference type="OrthoDB" id="432292at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056790">
        <property type="entry name" value="Ribophorin_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008814">
        <property type="entry name" value="Swp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640">
        <property type="entry name" value="RIBOPHORIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25147">
        <property type="entry name" value="Ribophorin_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044188274" evidence="8">
        <location>
            <begin position="19"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II C-terminal" evidence="9">
        <location>
            <begin position="172"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF88482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="275" mass="30373" checksum="9E76410CFA342263" modified="2022-05-25" version="1">MQLLKALTLFAIATLGQAFTVKDVHFAFPDTNERGIMVGTINSNSDKLERPIRLDTLDQTIELNFASDARLSQATLLLGLPDIGLEQAIQATLTENQGLLLYRFRIPVNQLSQKLLHAAVNEGKLLTGSVVLAESSDGILSTLFDLELDVNETVAYTKPDRFEAKPEIYHVFRPEPQTVSWAFAQIFSFAIVVATLGLVISWMSAGALTFGSVPHGLNFIYFLAFIGSVMGFEFIFASYYTGTSIFDTLKHAFLLSIPALLLSTKFLRSFGKELH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9D9</accession>
    <name>A0A8J2X9D9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="20">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">BN860_04434g</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 20">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Membrane</location>
            <topology evidence="20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="20"/>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000026">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000087">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Phospholipid-transporting ATPase"/>
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    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
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    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
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    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
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    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 19 20" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 18 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 20" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="438"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="488"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1000"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1041"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1080"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1123"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1152"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1195"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="23">
        <location>
            <begin position="177"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="22">
        <location>
            <begin position="283"/>
            <end position="342"/>
        </location>
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        <location>
            <begin position="972"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1313"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="17">
        <location>
            <position position="552"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="552"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="552"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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        <location>
            <position position="553"/>
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        <ligand>
            <name>ATP</name>
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        <location>
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        <location>
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        <location>
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        <location>
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        <location>
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        <source>
            <dbReference type="RuleBase" id="RU362033"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        </source>
    </evidence>
    <sequence length="1334" mass="151215" checksum="92B90B3CBA374AF5" modified="2022-05-25" version="1">MSVPSSSKQGPDDVLFDIDFLNDPPATESHGEQNTPEFLIHDETNRYNANYLLPQETIDLNRDESIENDVAANPFMDDTAATWNDDFFNGASHGSRLKRNKSTRGGNFISRAFGRLRDRIGKNKKRYQSFEMTNYNDRDNHDNYEKSRNMFDIKVLFNHYILRKPLGNENDGGPRLIHLNDSIANGALGYGDNHISTTKYNAATFLPKFLFQEFSKYANLFFLFTSIIQQVPHVSPTNRYTTLGTLMVVLVVSAIKECVEDIKRVHSDNELNNAVTEIYSDLDGGFIEKRWIDVRVGDIVKVKSEEAIPADMIILSSSEPEGLCYIETANLDGETNLKIKQARVETSKFIDAKTLGDLHGEIRSEQPNSSLYTYEATMILNGQEIPLVPDQMILRGATLRNTAWMFGIVVFTGHETKLMRNATATPIKRTAVERIINLQITVLFGVLIVLSLISALGNVIMSTAYSKHLPYLYLKGTNKVGLFFRDLLTFWILFSNLVPISLFVTVEVIKYYQAFMIGSDLDLYDEETDTPTVVRTSSLVEELGQIEYIFSDKTGTLTRNVMEFKSCSIAGRCYIETIPEDKAATYEDGIEVGYRKFDDLEKKLNDPADDESPLIKEFLTLLATCHTVIPEFQSDGSIKYQAASPDEGALVEGGAYLGYKFLIRKPSSVIVQIEETGEEKTFELLKICEFNSSRKRMSTIFRTPDGSIKLYCKGADTVIMERLASDNNPYVDSTVRHLEEYASEGFRTLCLAMRDISDAEYSEWSKIYESAATSLEDRQQKLDDAAELIEKDLILLGATAIEDKLQDGVPETIHTLQEAGLKIWVLTGDRQETAINIGMSCRLLSEDMNLLMINEDSKEKTSDNMIEKLDAINEHKISPQEMDTLALVIDGKSLEFALEPDLEDYLLALGKICKAVICCRVSPLQKALVVKMVKRKTSSLLLAIGDGANDVSMIQAAHVGVGISGKEGMQAARSADFAIGQFKYLKKLLLVHGSWSYQRISVAILYSFYKNIALYMTQFWFGFSNAFSGQSIMESWTLTFYNVFFTVLPPFVIGVFDQFVSSRLLERYPQLYKLGQKGQFFSVTIFWGWIMNGFYHSAVIYVGSILFYRNGAVLNKHGEVADHWSWGVAVYTTSVAIVLGKAALVTNQWTKFTIFAIPGSFIFWIVFFPIYATIFPHFNISREYYGVVSHTYSSATFWLMLIVLPVFALMRDFLWKYYRRMYVPQAYHVVQEMQKFNISDYRPHVQQFQSAIRKVRQVQRMKKQRGFAFSQSEQGGQERIVRMYDTTQKRGIYGELHDASANPFSDDNYVNIPSNPFSDENQEPALVDGNNTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9I2</accession>
    <name>A0A8J2X9I2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ZYBA0S08-03928g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_03928g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316461">
        <property type="protein sequence ID" value="CDF90823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X9I2"/>
    <dbReference type="OrthoDB" id="952271at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:metalloendopeptidase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:peptide catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Protease 3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
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        <property type="entry name" value="Metalloenz_LuxS/M16"/>
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        <property type="entry name" value="Pept_M16_N"/>
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        <property type="entry name" value="Pept_M16_Zn_BS"/>
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        <property type="entry name" value="Peptidase_M16"/>
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        <property type="entry name" value="Peptidase_M16_C"/>
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        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
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        <property type="entry name" value="PqqF-like_C_4"/>
    </dbReference>
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        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PqqF-like_C_4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="10">
        <location>
            <begin position="36"/>
            <end position="157"/>
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            <end position="773"/>
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    <feature type="domain" description="Coenzyme PQQ synthesis protein F-like C-terminal lobe" evidence="13">
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            <end position="959"/>
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            <end position="267"/>
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        <source>
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            <dbReference type="RuleBase" id="RU004447"/>
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    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="10">
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        <source>
            <dbReference type="EMBL" id="CDF90823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1189" mass="135774" checksum="0B14F9375DE921E6" modified="2022-05-25" version="1">MSVKHLKYYDVPFLTPFSFEGRAHKLCKLPNGILTLLISDPTENSSACSLTVATGSHNDPIEVPGLAHLCEHMVLAAGSRKYPDPGLYHEKILKNGGSQNAFTTGEQTTFYFQLPNIYHSLERGFEEALDIFGSFFEEPLFNSTIINKETYAIESEHGLNTSTNSKIFYHGVRLLANENHPFNRFSTGNISTLSSIPHLKGLNLKSQLHEYFKENYCATKMTLCLRGPQSIHSLARLAANNFSQIRMHPSQGRFSPPRSPTKWRKSPCEEQKDVTLEKMRILRGTWLPKYHGNLCFMPDQEQKVLFIKSSKNPMVRFVFPAVEKSTRFTSKDLKLMTHFWPELLGDDSHGSFSHWLTKRNWITCCYAFVSEIATGNSSLILELDLTKSGFQNIPYIVKILLKQIVPAFSQKFTLELARFLSEQNCIDLIRFLYQSAEISPMEECAKLSCLLQDDLEAIDPACIFRGFPMLIDIKNQTIGNYGENKESDNWWIGQAIKFQNFLKQFMTLSNMRMIILGDITESTLGFTHSDLITDVFYEFEYCKRKHVTNPKEELVTDYSFTIPPPNCFIPSWAGSYPHLKNMLIESSAKSQSVPLSCTIQNTAHKAIPHLASQNSNHEMWVLPDEDDVSLHLKCIVSFEIFALDLEASPQSTMNLEILGQIISISLLPQIYPSLKLGFTCELIPSKKGDVRLRFTICGFSEQLITVVKFIIEIIRKISEDPSFPSKETFRRARILVRNNYERAADENCANIGSIGLYIVLEKYMWSLEDRLDALEEIDLFSLKKFCASFIESTKYLSLFVQGDLSCADELNSYLHRNFTQHLGGGIYETCGKNFLIKTTKILEPGTNLCVQHDGQKDDPNNSIVYFIQTGHRNDKDAFTLSSFTAFLLSLTLTKELRTKRQVGYLVTGGLRLLSDSIGLHITIMSSGSPLDLETKIDQYLWYLESQIEKLTEEEFRKEYVRVYLRLLDGNCSGDVNESSGPTDLLSELVANVQMGDSEILASPTMKRHRRLQNQIIQEQYNFSTQGESIDCHLLTNMPLKQYLEFFRTYITVNSTKRSKISIMIACSMAEKEIINRKIFLQLETFLKVKGFAIPVDDLQKIVERSKGKPKNLALELYSSFHARNEARRLCSVVLAELFKMLAMSLRHRYGNLSRDDTSRKDSQEWNSNVKSALELRVIEDINLFKRVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9M8</accession>
    <name>A0A8J2X9M8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="17">ZYBA0S03-00562g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BN860_00562g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:histone binding"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:chromatin remodeling"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17996">
        <property type="entry name" value="DEXHc_SMARCA2_SMARCA4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SF2_C_SNF"/>
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    </dbReference>
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        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000843">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Bromodomain"/>
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        <property type="entry name" value="Bromodomain-like_sf"/>
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    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
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        <property type="entry name" value="Gln-Leu-Gln_QLQ"/>
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        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
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        <property type="entry name" value="HSA_dom"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="SnAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51666">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="QLQ" evidence="16">
        <location>
            <begin position="153"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="HSA" evidence="15">
        <location>
            <begin position="548"/>
            <end position="624"/>
        </location>
    </feature>
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        <location>
            <begin position="737"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="1049"/>
            <end position="1212"/>
        </location>
    </feature>
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        <location>
            <begin position="1495"/>
            <end position="1565"/>
        </location>
    </feature>
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        <location>
            <begin position="56"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="212"/>
            <end position="250"/>
        </location>
    </feature>
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        <location>
            <begin position="292"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1296"/>
            <end position="1320"/>
        </location>
    </feature>
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        <location>
            <begin position="1333"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1615"/>
            <end position="1651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="94"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="350"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1344"/>
            <end position="1358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1411"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1424"/>
            <end position="1453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CDF88728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1651" mass="188533" checksum="8BBE1D270D7285F7" modified="2022-05-25" version="1">MNLEIPQRQFTKEEINRCYLRWQQLRNEHGENAPNVPEFIYFTKVLHVAAKQQQEQQQQQQQQQQLQQRSQPEEQQQQPQDSGQNGLPQLQTLSQHKQGSVSSQSGQHSVPPASQAATQVGSSPQQQSQTQRQPPQPQQHAQNVPQTPHHSSIFTPDQSELLKAQITSLKCLVNQKPVSVECQNVIQQSISNAPDFKKMLMALSEFVKQRQLQQHSTVNGGGQSPSQQQSYQQQQQAPRLSQQPTSVNQHLSPQMANARLQQLQLKQQEQLQRQQLQKQQLQQQQQQEQLRNQHLQQQQREKLIKAQASAGATPSVEGNVSSSSVSGTPNALVGTPSGIHPKEEREHSLGSVNAQPQKLTHSNDISDTQKHQVLPDGISEPPKQRAPLPLKQFIEENKEIGRVVDTDDPDIMVDSYSEPPELNDKIDYDKLFTSSSKSKLLITPGLLPVGIDIHTAEEIYQTLIALNLDTAVDDCLNKILDDSAAEEMKEQSVYDYYALQLLPLQKAVRGHVLQFLWYQSSLLTSTHPNFLSKIRNINVQDALLTGELYKKHETLQYEKKRVEKIKKLEAVRKSCVNIYNYRLDNRIRRVKFGHRMVGVHANIEKEEQKRAERKAKERLIALKANDEEAYIKLLDQTKDTRITHLLKQTNAFLDSLTRAVKDQQEYTKDMIDSHLTEESAEKDEVNPQMHEEDIEEQSNVDYYNVAHRIQEEIKEQPSILVGGQLKEYQMKGLQWMVSLFNNHLNGILADEMGLGKTIQTISLLTYLYEKKNIKGPFLVIVPLSTLTNWSSEFEKWAPMLRAISYKGAPNERKAKQAVIRSGEFDVVLTTFEYVIKEKAVLSKPKWIYMIIDEGHRMKNTQSKLSLTLNTFYHADYRLILTGTPLQNNLPELWALLNFALPKIFNSVKSFDEWFNTPFSSAGGQDKIELSEEEMLLVIRRLHKVLRPFLLRRLKKDVERELPDKVEKVIKCKMSALQQVMYQQMLKHRRLFVGDQANKKMVGLRGFNNQIMQLKKICNHPFVFEAVEDQINPTRETNATIWRVAGKLELLERVLPKLKATGHRVLIFFQMTQIMDIMEDFLRYIGIKYLRLDGHTKSDERSELLSLFNDENSGYFCFILSTRAGGLGLNLQTADTVIIFDTDWNPHQDLQAQDRAHRIGQKNEVRILRLITQNSVEEVILERAHKKLDIDGKVIQAGKFDNKSTAEEQEALLRSLLDAEDEKRRKREMGIDEEEEVNDVEINELLARNEGELSVFAQVDAERSRKALEMGITTRLMENNELPEIYHQNIDVELEREDSESVAAYGGRGARERKTTTYSDNVSEEQWLKQFEISDEEEEVGASSAKRRKASKSRAANKKVKLEQEPAEIVTEDPTPVSTNDSAMHKDGESGVDVTSEFGAASEETEEDAVSGSASTPAGISGKSTKNKGRVRSRKNPISRSRPVRGRGRGRPPKPRAGLEYQRDPSAATQPHEIRNQVASVARELYTYALECTSEDDIRLSDIFLEKPSKELFPDYYQLIRYPIAFDTINSAIEAFAYDSLKEVLEDFHLIFSNARIYNTEDSLVYLNSVELEQVVTSKYRELSKDEKPLDFSKFDESFATKPLNLSQILSADVIPDTMDDSSPPVDNKISATEPSVTPHPTEKNEEDEVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9X9</accession>
    <name>A0A8J2X9X9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN860_02322g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="HG316462">
        <property type="protein sequence ID" value="CDF90990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02584">
        <property type="entry name" value="RNAP_II_Rpb1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02733">
        <property type="entry name" value="RNAP_II_RPB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.30:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.390:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.100:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.140:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.180:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000684">
        <property type="entry name" value="RNA_pol_II_repeat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007075">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007073">
        <property type="entry name" value="RNA_pol_Rpb1_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038593">
        <property type="entry name" value="RNA_pol_Rpb1_7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006336">
        <property type="entry name" value="PRK08566.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF37">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04992">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04990">
        <property type="entry name" value="RNA_pol_Rpb1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05001">
        <property type="entry name" value="RNA_pol_Rpb1_R"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00115">
        <property type="entry name" value="RNA_POL_II_REPEAT"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 15" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="17">
        <location>
            <begin position="232"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1532"/>
            <end position="1737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1535"/>
            <end position="1558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1566"/>
            <end position="1725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1726"/>
            <end position="1737"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CDF90990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1737" mass="192368" checksum="ABD674D61BACD6AC" modified="2022-05-25" version="1">MVGQQYSSVPLRTVKEVQFGLFSPEEICAISVAKIRFPETMDETQTRAKIGGLNDPRLGSIDRNLKCQTCQEGMNECPGHFGHIELAKPVFHIGFIAKIKKVCECVCMHCGKLLLDEHNEQMRQAIKIKDSKKRFNAVWSLCKTKMICDTDVPSDDDPTHLVSRGGCGNAQPTVRKDGLKLVGSWKKDKGSDDTDEPEQRVLTMEEILNIFKHISPEDSWKLGFNEEFARPEWMILTVLPVPPPPVRPSISFNESQRGEDDLTFKLADILKANISLETLEHNGAPHHAIEEAESLLQFHVATYMDNDIAGQPQALQKSGRPVKSIRARLKGKEGRIRGNLMGKRVDFSARTVISGDPNLELDQVGVPKSIAKTLTYPEVVTPYNIDRLTQLVRNGPNEHPGAKYVIRDNGDRIDLRYSKRAGDIQLQYGWKVERHVIDNDPLLFNRQPSLHKMSMMAHRVKVMPFSTFRLNLSVTSPYNADFDGDEMNLHVPQSEETRAELSQLCAVPLQIVSPQSNKPCMGIVQDTLCGIRKLTLRDNFIDLGQVLNMLYWVPDWDGVIPTPAILKPKPLWTGKQVLSIAIPKGIHLQRFDEGTTLLSPKDNGMLIIDGQIIFGVVDKKTVGSSSGGLIHVVTREKGPQVCAKLFGNIQKVVNYWFLHCGFSTGIGDTIADGKTMRQITEAISEAKRKVEEVTKEAQANLLTAKHGMTLRESFEDNVVRYLNEARDKAGRLAEVNLNDTNFVKQMVSSGSKGSFINIAQMSACVGQQSVEGKRIAFGFVDRTLPHFSKDDYSPESKGFVENSYLRGLTPQEFFFHAMGGREGLIDTAVKTAETGYIQRRLVKALEDIMVHYDGTTRNSLGNIIQFIYGEDGMDAAHIEKQAIDTIGGSDKNFEKRYRIDLLNPEYALDPSLLESGSEIMGDVKLQSLLDDEYKQLVEDRKFLRQIFVDGESNWPLPVNIRRIIQNAQQTFRIDHAKPSDLTIKDIVWGVKELQERLLVVRGKSRIIKEAQNDAITLFCCLMRSRLASRRVIQEYRLTKQAFEWVLNNIEVQFLRSIVHPGEMVGVLAAQSIGEPATQMTLNTFHFAGVASKKVTSGVPRLKEILNVAKNMKTPSLTAYLEPDYAADQEKAKLIRSVIEHTTLKSVTVASEIYYDPDPRSTAIPEDEEIIQLHFSLLDDETEKSLAQQSPWLLRLELDRAAMNDKDLTMGQVGEKIKETFKNDLFVIWSEDNAEKLIIRCRVVRPKSMDLETEAEEDHMLKKIENTMLENITLRGVENIERVVMMKYDRKVPSKTGEYQKVPEWVLETDGVNLSEVMTVPGVDATRIYTNSFIDIMEVLGIEAGRSALYKEVFNVIASDGSYVNYRHMALLVDVMTTQGGLTSVTRHGFNRSNTGALMRCSFEETVEILFEAGACAELDDCRGVSENVILGQMAPIGTGAFDVMIDEESLVKYMPEQKITELVDGQDGGATPYSNESGLVNTEVEVKDELMFSPLVDSGSTDAMAGGFTAYGGGSTSFGSYGDAPISPGFGGVSSPGFSPSSPTYSPTSPSYSPTSPSYSPPSPNYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPGSPTYSPGSPKQEDEEKKPEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XAG7</accession>
    <name>A0A8J2XAG7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Glutamate synthase [NADH]</fullName>
            <ecNumber evidence="19">1.4.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">BN860_02520g</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Forms L-glutamate from L-glutamine and 2-oxoglutarate. Represents an alternative pathway to L-glutamate dehydrogenase for the biosynthesis of L-glutamate. Participates with glutamine synthetase in ammonia assimilation processes. The enzyme is specific for NADH, L-glutamine and 2-oxoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>2 L-glutamate + NAD(+) = L-glutamine + 2-oxoglutarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="1.4.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="24">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="24">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (NAD(+) route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Energy metabolism; nitrogen metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Nitrogen metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.14" evidence="19"/>
    <dbReference type="EMBL" id="HG316463">
        <property type="protein sequence ID" value="CDF91225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4327079at2759"/>
    <dbReference type="UniPathway" id="UPA00045"/>
    <dbReference type="UniPathway" id="UPA00634">
        <property type="reaction ID" value="UER00690"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016040">
        <property type="term" value="F:glutamate synthase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019676">
        <property type="term" value="P:ammonia assimilation cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097054">
        <property type="term" value="P:L-glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00982">
        <property type="entry name" value="gltB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00713">
        <property type="entry name" value="GltS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000207">
        <property type="entry name" value="Glutamate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000031">
        <property type="entry name" value="Glutamate synthase 1 [NADH]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000017">
        <property type="entry name" value="Glutamate synthase [NADH], amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000113">
        <property type="entry name" value="Glutamate synthase [NADH], amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000124">
        <property type="entry name" value="Glutamate synthase small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.60:FF:000001">
        <property type="entry name" value="Glutamate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000001">
        <property type="entry name" value="Glutamate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="entry name" value="Glutamate synthase, alpha subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028261">
        <property type="entry name" value="DPD_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006982">
        <property type="entry name" value="Glu_synth_centr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012220">
        <property type="entry name" value="Glu_synth_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006005">
        <property type="entry name" value="Glut_synth_ssu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051394">
        <property type="entry name" value="Glutamate_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01317">
        <property type="entry name" value="GOGAT_sm_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008730">
        <property type="entry name" value="PRK11750.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43100">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43100:SF1">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14691">
        <property type="entry name" value="Fer4_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00310">
        <property type="entry name" value="GATase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04898">
        <property type="entry name" value="Glu_syn_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000187">
        <property type="entry name" value="GOGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="Alpha subunit of glutamate synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18 24" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0288">FMN</keyword>
    <keyword evidence="17" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="13" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="15" id="KW-0408">Iron</keyword>
    <keyword evidence="16 24" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="25">
        <location>
            <begin position="52"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="23">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1179"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1185"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1190"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00057049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00068518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR000187-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR000187-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="CDF91225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2135" mass="236101" checksum="44AC9C9C310848B0" modified="2022-05-25" version="1">MVLKSEVYDPLQEAYEGGSPLELSEGHVNKSWANVIPDKCGLYDPDYEKDACGVGFVANIKGEPSHKIVSDARFLLCNMAHRGAVSSGGNGDGAGILVGIPHEFFQREFKLDLGVDIPAKGQYAVGNVFFKKDQGSALQAAQSIFEDLAIEHGLKVLGWRSVPRDSSILGEVALSREPTILQPLVVLSERDADEPFDEKLFQTRLYILRKLATNEIGIENWFYVCSLSNRTIVYKGQLTPAQVYNYYHDLTNAHFKSHMALVHSRFSTNTFPSWDRAQPLRWLAHNGEINTLRGNKNWMRAREGVLVSETFKDQLDKLYPIIEEGGSDSAALDNVLELLTINGTLSLPEAVMMLVPEAYHKDMDSNLKAWFDWAACLMEPWDGPALLSFTDGRYCGAMLDRNGLRPCRYYVTKDDRVICASEVGVIHVDNSLVVQKGKLKPGDLFLMDTEKGEIVDTKKLKMEFAKKQDFKSWLSKVIQLEDLVTKNEKYLPDEYVPECSDLKVQNDPRLLANGYTFEQVSLVLTPMALGGKEALGSMGNDAPLACLNEDPVLVYDYFKQLFAQVTNPPIDPIREANVMSLECYVGPQGNLLEMHPSQCERLLLKSPILHWREFLAIQNIEKAHPTWSVANIDITFDKSEGLLGYTATIERITASASDSIDQGKKILILSDRKLGPSRVAISSLIAVGAIHHHLVRNKQRSHVAIILETGEAKEVHHFCVLLGYGCDGIFPYLAMETLIRMNHEGLVRSVDDDDLQVDDHVLFENYKHAIDGGIRKVMSKMGISTLASYKGAQIFEALGVDNSVIDLCFAGTASRIKGVTFEYLAQDAFSMHEMAYPSRPVVSNSVALPEDGEYHWRDGGYKHVNDPQAIASLQDTVRTKNETSWDMYVKRESEAIRDCTLRGLLELDFEGSTAIPLEQVEPWTEIARRFATGAMSYGSISMEAHSTLAIAMNRLGGKSNCGEGGEDAERSIVHENGDTMRSAIKQVASARFGVTSYYLSDADELQIKIAQGAKPGEGGELPAHKVSKEIAKTRHSTPYVGLISPPPHHDIYSIEDLKQLIYDLKCSNPRANVSVKLVSEVGVGIVASGVAKAKADHILVSGHDGGTGAARWTSIKNAGLPWELGLAETHQTLVLNDLRRNVVVQTDGQLRTGFDIAVAVLLGAEQFTLATVPLIAMGCVMLRKCHLNACAVGIATQDPYLRSKFEGQPEHVINFFYYLIQDLRKIMAKLGFRSIDEMVGHSEKLRKRENVSTKAINIDLSPILTPAHVIRPGVATKFTKKQDHRLHTRLDNKLIDEAEVTLDRGLPVTITAEIINTDRALGSTLSYRISKRFGEEGLPQDTVVVNIEGSAGQSFGAFLAPGVTFILEGDANDYVGKGLSGGIMVIKPPRGSTFKSDENVIIGNTCFYGATSGTAFISGCVGERFAVRNSGATMVVEKIKGNNAFEYMTGGRAVVLSQMESLNAFSGATGGICYCLTSDYDDFVGKINTGTVELESLSDPVEIAFVKNLIQEHYNHTKSELTAKILSKFNYYLKNFVKVIPTDYKKVLLKQKEEKLKQKRLADLAYLQKFQRSSNSNLDATNGDVDALMSTKREQNNAAISFKSTRKEPGVADLEDAVPNHKQLAKNMEKLEKVRGFMKYKVRHQSKRPPAVRSQDWKEFTNNLNKKDAKYQTARCMDCGTPFCQSDTGCPVSNIIPKFNDLVFKNQWKLAWEKLSETNNFPEFTGRVCPAPCEGACTLGIIEDPVGIKSIERLIIDNAFKEGWVQPSPPKHRTGHRIAVIGSGPAGLACSDQLNRAGHSVTVYERADRCGGLLMYGIPNMKLEKSIVQRRVDLLADEGVEFVVNTEIGKDISVTELRERFDAVVYAIGSTIPRDLPIKGRELKNIDFAMTLLTNNTAALLNKDLEAVRQAIAGKKVIVIGGGDTGNDCLGTSVRHHASSVLNFELLPQPPKQRSRDNPWPQWPRVMRVDYGHSEVKEHYGRDPREYCILSKEFIGNEEGEVKAIKTVRVEWKKSESGVWQMVEIPGSEEIFEADIVLLSMGFVGPELVEDPTVRKSRRGTICTVNDASYNVDDGKTFAAGDCRRGQSLIVWAIQEGRKCAAAVDQMLMGVTGLPGNGGIVKRNFAMLEELAATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XAP2</accession>
    <name>A0A8J2XAP2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ZYBA0S04-06766g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_06766g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the THADA family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="73997at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056843">
        <property type="entry name" value="THADA-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056842">
        <property type="entry name" value="THADA-like_TPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019442">
        <property type="entry name" value="THADA/TRM732_DUF2428"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051954">
        <property type="entry name" value="tRNA_methyltransferase_THADA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387:SF0">
        <property type="entry name" value="DUF2428 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387">
        <property type="entry name" value="THADA/DEATH RECEPTOR INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10350">
        <property type="entry name" value="DUF2428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25150">
        <property type="entry name" value="TPR_Trm732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25151">
        <property type="entry name" value="TPR_Trm732_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26523">
        <property type="entry name" value="Trm732_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like TPR repeats region" evidence="4">
        <location>
            <begin position="225"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2428" evidence="3">
        <location>
            <begin position="602"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like C-terminal TPR repeats region" evidence="5">
        <location>
            <begin position="828"/>
            <end position="974"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF89540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1415" mass="164180" checksum="85B456989A77342C" modified="2022-05-25" version="1">MIDQNKVLAIKEFLLEHNPSKIENESVFEGYIGGFFDLFETFEHQTEGITDSFRLLIVDTFSIWILRSTQCLRSKKVDTAEFLSQLQNMLLTKKNSEIIFQYLTDFWTDGTTAFNNALREMFTKLLRLLDIVYSEQDRSILFNSWLEQILNIRSTLKVQYHLIDSLAPATDLYSVLQKRPDFIETSLSLMWSDALSNPIGKCLTSLLLNIYRIHYNQNESMLDQWLNLWQNPVLRIMDDLRYTKAIELYILTPLFREMPKKTFVKFIERCSTCKVSVSILLLRIGQELVIEEEPYHHDKLISLETVEQLLQQDNNKLHAFELLTFSSKTSKPVHPYIFEVIKRNLRIFFVDTNIETRNYFCSSFKHFVLRIRDSAYALNRDREKLIKAKKFPEEQDDKLKQIMQSEDFLRWLFSFLKTQICPGTQYQRNTLAFKILVLLLESGIDDTISEKYLDSRNLRTYPFSIQFFEDGILIRSLIDNLTSNYNDIRRFAKILLTMAFHSENCNSLRNEFDWKNLHQRAEGYMEDYQNTEIGAALQDFLFLTSSDKKAFMDRLLGRLQEKINESKKDYLKYHHDPVGGYFATLALLLKNDEFTSESYYNIITRCLSLILNNWDAVKGPVCCDPTEAKTALEYSKDGIKDQYIAAKAFRSIKESSALLEVIIARPSTTFEQLKTAGDFLICQLSNLRHSGAFQSVLPSFNACCSKCSAQYPNQLEVWLHIVIESLEVKTQMVTRRSGGIPYLLTAILASEKGRERPLLKFAFENLERIASLRVTQYKDEVDLPQVNAFNCIRSIFIESKLAEPCAAYVASALNLSLKNFTSNIWSLRNCSIMLFTSLQNRIFGKAGKSVSARLFFTRYYGVKELLLNVLKASLKEPTDSSNTTMSQSHVESIFLVLNLLLRLQPTPGYSGLEVYYKKVYECLESCHWHIREMAAKTMASLNGYSGTECIIMMEGVSTKNQNKLHGHLLVVKNILERNKELVQSKNSMIELFDLLLARCEEFLILNTCFATAKAYIEVLEFVLNSEHFEISKAVRNSMLTNLSQFFISHNEKYTVDGMKQLCLAMTLRMLFKHEYAENIPYLCRLSIISEFQRVQSTAIQYIVEEIDLTAPTNRETLIELKRSFMDPNTATSLKPLILRALRKIGDEITFDTVAELIREPNNEELQLSAIETLGCLAKARDAKFIEPLIGKYSNDDMPTDFRMASLKCSTNFSKNYKLPSILLQVHKMLNDDDDEIRKQAAEFLNKFFFRNIYVLSPSVTAAKFGQVFCREFSTIDDAREDVVRKLTLFWSSYDIFAPASTGFDGLFEVEKDNQFRNDIEQNIQYINILKRWHFESIEFSCWVRYCVERLLTFLKDSETLDGPLGWSSHADVFPRLCIMWNLAQTYATSLVDELEKSLRTRNVHPLIFDYLFIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XAP5</accession>
    <name>A0A8J2XAP5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S12-02740g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_02740g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316465">
        <property type="protein sequence ID" value="CDF91603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="19311at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11210">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018515">
        <property type="entry name" value="Tuberin-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027107">
        <property type="entry name" value="Tuberin/Ral-act_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024584">
        <property type="entry name" value="Tuberin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10063">
        <property type="entry name" value="TUBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10063:SF0">
        <property type="entry name" value="TUBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11864">
        <property type="entry name" value="DUF3384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03542">
        <property type="entry name" value="Tuberin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50085">
        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rap-GAP" evidence="2">
        <location>
            <begin position="1131"/>
            <end position="1359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF91603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1361" mass="155266" checksum="D3EF55203CDE3355" modified="2022-05-25" version="1">MPLPGYVFKKGTNGLELLRTVSNSVNAETHSDTKIRRPSEKYFERVLLKIDDRSLDYAERTQAVVEMRGLLGVLDSNIIPEIWFATHSLIAAGVPRATRRSALELLQGCISHTRDDLIKSIFFQIIYDCLVPPGKEEKLSTIDPDLDLILHCFNEIASNADDLLTNEEKELMTEIVADLINLPLQQDPGYKFLPVILELLDLSEKLSIPLNSSDVGTFLDTLGKISETANRANMRFQTASISFIKHYQNGIKLESLDDVALILINISAIMAQDKVDAILRPLLQLMVDTAQNQAIAEKFCMLPLRKPIDRGVLVKCYASVYVEAFAKGKISPQEVFAFLNKLFVAKLDSELLETSASLLSNEAFVTNWKMAEQTTKISLWPMIEKALVRTKWSLPQVIDSLLRSMTCHPTLIPMVPEEIIHFICQSKLPINFENLMFFLEDRIANIREVKTMQQLTNFVVQGGVSTTFRLSLIQFFKLWLEENKFNLANFEPDLADCIWSFYHKFETMSTDDRELESFGELLYIFMQLSSLQLFESFITCHLLPTIRKILLHKSRRRSFVGSIGTFGRGKTFSKVRLKILPILVKNLVKAFIWSPLERTGGKCCLSFEALISVYEYAEQCKDSTILLIIARSMVRIRRDGEGAFYFADPEDAVGISSAFGRHREQIPEEAQVQWTFPETLDFIDTTFLGKRNECIKFEIRNGDTPSGKIKMGVWLSLAVSTIEDPVDLEIYSYLLTHLCSQLSELTLFEGHYKGINTFKNVICQHLRQSLPSTVKPSDTLSRSDLNSAYVRNLSAVLAYHRYEPKIFADDLISALVFGLTSWEKTLIPILHILTVSCFEIPTSVKRYITPILLELQKRITTLHAVPSILEFLLALKSSPPLISNLTPNETKRIFAVVFTLLENSVYLKMRSKSFSQNPGVSPQRLQFSSLPQDYEVEISPSTESFLVGDSMTRFFQYQSFTVLANWLFSVKGDQMNDLIQFVMKGLEKLAIIDDLKYDSLAYMDFVSRLNFSTQEEVPLLRGKDDHEYNDSSHLGRWISGSCLISIRTWDKTDRVVVELKRPSSESIFEIKLFEQPHQPKYTLFDFQKEDEPKAKSFPSLKPEQVLHLVHIQGIRKNLFRLPNNAAFNRSVNILERIPKVEFQKTGIIYIGPGQCTEFEVLNNSSGSHQYNWFLTQMGDFIKLKETSQFFYRGGLDPETDGEYALVWNNPNTQITFHTVTLMPPNCDLSFKKRHVGNDFVNIFYNDSGLYTFNFNLIKSQFTFINIVITPEFNGSNNKKISQHYKVKLYRRSGVPGLLSCAHFKILDKSNLARYVRHVSLIANALAEKYNETGSQDACSTWGVRCKQLLSIRDRAAQLAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XAV3</accession>
    <name>A0A8J2XAV3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S04-10726g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_10726g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XAV3"/>
    <dbReference type="OrthoDB" id="410651at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808:SF2">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="2">
        <location>
            <begin position="192"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="601" mass="68889" checksum="A973DF10A3918DB2" modified="2022-05-25" version="1">MLYINVNNIFFKSYSVDPVSGHAIYVFDSTYLPSSDEIADKQVYDLLIDKLMDKLITKIPSAPFILVVFSSGFLEKKISWVYGIKMFSKLPKELRAQLQKTYIVHESFFIKTVYQVLTNALNIKNLGNNALLDFNQSKNSLDSLQNASIIHVADLTDLSALVDITRLRISLNVYLYDYQFSEYIDVPKEYFNRLSAVGTRQYRQLIFDKIFRRLELDAIKVELVFQRPGSYKRVNILLDVIERNNYIDLSQWDIYSLATVFLHFLKHKSLPLIPIDLITLPISDDFEYTYATFCSIIKHNQYYELLETIFPLFISQLEHSEVTKHDARSLSKALCPQLCQEKISMMSADRLAVGTRFIRNLLEHFPAITQRIDRARLGITRGASPIKRVDRVKQETRPTSPKSLSQPEPPKPRKLIVKTPSNRFTGGKPAQLMPTQKKSTMQEKPITLERPTSNPNDTRECLSSSTTMIGENSSTSVLKHEPHDDIRSRSTSLAASTETINQMVKDDQLGDTNNDCDTNVSQLAFDDKNPSLQKNQTIFHFDRQLQSKRKITVKNEDNKFSREGYSDIKAGSKVSRLAALYEERLQGLQAIQEIKQKGGYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XAY5</accession>
    <name>A0A8J2XAY5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GPI inositol-deacylase</fullName>
            <ecNumber evidence="10">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_00298g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in inositol deacylation of GPI-anchored proteins which plays important roles in the quality control and ER-associated degradation of GPI-anchored proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GPI inositol-deacylase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="10"/>
    <dbReference type="EMBL" id="HG316468">
        <property type="protein sequence ID" value="CDF91864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XAY5"/>
    <dbReference type="OrthoDB" id="348976at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050185">
        <property type="term" value="F:phosphatidylinositol deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006505">
        <property type="term" value="P:GPI anchor metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000056">
        <property type="entry name" value="GPI inositol-deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012908">
        <property type="entry name" value="PGAP1-ab_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039529">
        <property type="entry name" value="PGAP1/BST1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056824">
        <property type="entry name" value="PGAP1_TMD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15495:SF7">
        <property type="entry name" value="GPI INOSITOL-DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15495">
        <property type="entry name" value="NEGATIVE REGULATOR OF VESICLE FORMATION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07819">
        <property type="entry name" value="PGAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25141">
        <property type="entry name" value="PGAP1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25140">
        <property type="entry name" value="PGAP1_TMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="716"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="750"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="812"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="869"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="891"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="929"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="988"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1013"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase PGAP1-like alpha/beta" evidence="11">
        <location>
            <begin position="149"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase transmembrane" evidence="12">
        <location>
            <begin position="718"/>
            <end position="1028"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF91864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1040" mass="119310" checksum="1CC8644A6971614B" modified="2022-05-25" version="1">MGLKRVLTGLVRRGYNSWPGLLKVPFLQNSSIFSGRITRVDGNETKIEDCKGNSTLISLNQKQTRRCRYGMRLAFLTGIILTMIVAYLTIFGTFYGADSPRCQPVYMYPSYARIDGFDLRFTPLAQKYHLYLYREQGLDKDPMDNDTIQLDGIPVLFIPGNAGSFKQVRSIAASAAESFFTHTEDIENSYKSNLDFFAADFNEDFTAFHGRTMLDQAEYLNDAISYILSLYKETNSYNAPLPSSVIILGHSMGGVVARILPTLQNHIDGSIRSIITLSSPHAASPVTFDGDILKIYQQTNDFWRNEFQNNSSFMSQNVSLISVTGGVLDSTLPADYTAVEDIVPLTNGFTTFTTAIPNVWTPIDHLAIVWCNQLRDVVSHLLLETVNVNSSEKVLPLDQRMQISRRFLLSGLEDFAVQDFRVTNATKNLEKYEQQLFGDAEVKKSMEPFILSTRDIEVSSKFTKFILPKNEPKAFFSFMTSFPKAVIYFCRENKMVGENEEEYLECVNAQADILQIPESSKYTEYAGDSSIYGNSEPFKFFRFDSNILSQYDFVLLESPNMAELANDDFMIASVTQVENILAVNDTPLDILFFGFEITLSSDDSFWHTLSFQNLWESLISYRIHVSIYPEVEKDLLFQPFIRQWNNDPAESKWHVNVGHSDQDVFMHNVAPFIPLEETHDKALKLTLFLPPDCKVRLHVAFNWRMTLKMLFIRYRLTFLALPISLISITMAYQFFWYYKTSLFMDFGAALSLLFYKHWLLGAVTLVVLSPLANTDIGQKIFYTLDPIGFNRPFLTTDVHMRTNFYYLGIRSWFMSGLGIFFAFMTIGVLLLVFQLFQILEKILHILKRVKLLPIPDVEFRHHKMVHLNLSRLFVSFLLLLAVIFYIPYQLAAAICLLIQIGTCLRIALMAENVTSKEYSNLRNYNFSMFLLMAFVVAVDAPIIIVFLHNIAIKWETPFRSHHNFMAIAPMIFLVGINSQFNVPRYTKLDVTITIGLLVYTGFFSLIYGTRNLYWTHHLCNIICAWLLYGMRFTFGKELIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XB18</accession>
    <name>A0A8J2XB18_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Altered inheritance of mitochondria protein 21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_02476g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in mitochondrial migration along actin filaments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIM21 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316466">
        <property type="protein sequence ID" value="CDF91740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XB18"/>
    <dbReference type="OrthoDB" id="3995855at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021582">
        <property type="entry name" value="Aim21"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11489">
        <property type="entry name" value="Aim21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
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        <location>
            <begin position="125"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="6">
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    <feature type="region of interest" description="Disordered" evidence="6">
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="955"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="847"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="127"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="259"/>
            <end position="284"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="285"/>
            <end position="296"/>
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            <begin position="399"/>
            <end position="408"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="6">
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            <begin position="431"/>
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            <begin position="520"/>
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            <begin position="623"/>
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            <begin position="651"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="670"/>
            <end position="686"/>
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        <location>
            <begin position="728"/>
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        </location>
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        <location>
            <begin position="804"/>
            <end position="813"/>
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        <location>
            <begin position="913"/>
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            <begin position="1002"/>
            <end position="1011"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00002092"/>
        </source>
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    <evidence type="ECO:0000256" key="2">
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            <dbReference type="ARBA" id="ARBA00004134"/>
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    <evidence type="ECO:0000256" key="3">
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            <dbReference type="ARBA" id="ARBA00006466"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF91740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1011" mass="114060" checksum="17D38EF1D02E8873" modified="2022-05-25" version="1">MSSEEFPPIPTRPQRHTASPSPTPTVPIRPASKPNTNSIPQVPTKRPTRTKTAELDELMINTDNELREMKGLIRNRTIDSRNQFQELKTQEDKLQSGIEELLGQPGSNVKEQELQDEKERLLRRRQSMLEERKKQQLAREEEMKTLERRREELLQERDRERELERKVEEERKRETELAKEAEENLEWQKEDDMEKEMQMERQKEVETERKLQKRKEIERQKESEEQLRQRKEEELARQRDLELEEKMQRDLERDQEDELQAKKKELELLENERQKTEQKEHLKNSQESSSVSTESVAGKTPFEGAYSDADSAFLDAVGKDFGLKPSESQTFPQMPERPRKTLVSSKASENKSFEKSPTPGPEQATANVKTEKDLPSSGSSESKSYETPFVPERPKIGTGDTSSDSCFSPPNIRPSASERRAESPSIPERPSAFSKKSSSESPTLQDNRPSVFEKKNDSPSVRERTSFVNKKPANEFPAIPARRPSEKSSESPTIPERPNIFGSNSSLGSPVFPDKRPDSNSRQSQSSPTDAEADETQSSPSILPPVPDRRPDFNNKKSPSPVAPSMPEKRPTTSLRQSTIPARPEPESHETTTDSLTDTNVVQKEMASSVEGEPDSQLQSKIRQPDPEEVGVDKTKTALPPEVENVDANETVPQKLSNNENSSASSIMDQKSREDSSEENFKEFSKKKVPPVPKKPSSRIFAFQEMLRKQQMEEMQNSRNQVPERGDNQSVSSPETLQQPSVPQRPHRPVNEERSKFANNLNGLFALPGMSPMGGGLPPSFAKKLNPSAKENKDTKPAPSVRQPRARGPRGRKLPSSVAAVEKVSSESKTNEIEIFKTWRTVMFKPVESSDNNIQELSQESANLKEISQEASKDDQENKVIEIADPTHCDLDDSQAVIPVEDTMHLEEISKVQNLSSSSIQDSVEVGAGPSSPNDDKLENYSEVFAETMNPMPATTFSFQEVSGNSRNSTTIDLAQTGDELNDPQSQVGHGEFIGDPKQEENCEEFADDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XBL0</accession>
    <name>A0A8J2XBL0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein kinase C</fullName>
            <ecNumber evidence="2">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">BN860_04016g</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="2"/>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XBL0"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20822">
        <property type="entry name" value="C1_ScPKC1-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20823">
        <property type="entry name" value="C1_ScPKC1-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08689">
        <property type="entry name" value="C2_fungal_Pkc1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11620">
        <property type="entry name" value="HR1_PKC-like_2_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05570">
        <property type="entry name" value="STKc_PKC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000101">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000103">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000034">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037778">
        <property type="entry name" value="C2_fungal_PKC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037312">
        <property type="entry name" value="PKC-like_HR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02185">
        <property type="entry name" value="HR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00742">
        <property type="entry name" value="Hr1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="REM-1" evidence="23">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="23">
        <location>
            <begin position="100"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="19">
        <location>
            <begin position="185"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="21">
        <location>
            <begin position="411"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="21">
        <location>
            <begin position="478"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="834"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="22">
        <location>
            <begin position="1094"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="309"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="544"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="724"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="309"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="328"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="561"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="616"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="724"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="863"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="CDF90241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1161" mass="131884" checksum="8B825907D2C38F30" modified="2022-05-25" version="1">MYSQVEQEIQRKIERERNIIQGASNLKKKTNNVMVIQKCNTNIREARQNIEYLEETFNKLHLKSETPQSKPEEDAKEKEFRQLSTISPNEHMYSRLDLVKYDCPSLSQRIQYMLQQLEFKLQVERQYQEANAKLTKLYQIDGDAQSSSAAEGGAQESKNRILLLTKALKKYQAINVDLDQFKQHDTETLNNQPKFRRKQLTGELHIGVTAIRDVDHIQSPLFSRKPESYITVKIDDTVKARSRASRNDRWNEDLVINVERGNEVEITVYDKVNDVMSPVAIMWLLLSDITEELRKKKVGQSSGQQGWVNASKVINSPPNGSVGSARRGSALSGTATASSSGGQGTSGNSTSNVHSNKISTSSWFTLEPAGQLLLTLAFNKSSQPVKKNIVGGLHRHGAIINRQEEVYEQHGHHFVQKSFYNIMCCAYCGEFLRYTGFQCQDCKFLCHKKCYGNVVTKCIAKTTTESDPDEAKLNHRIPHRFEPVANRGTKWCCHCGYILPWGRQKVRKCTECGIMCHTQCAHLVPDFCGMSMEMANKILRTIQDTKRSQEKRKRISPSLQTQSSSKTVVGSGSSPGKSGQRSSHSHRKRKSQTNVPEIKAPNKEQRQEALPPVTKPSERPKAKKAEATDKLSKFIDQNEAYLNFTENAHKSDVPLEAAGRTLDPFASNDISSDNPYLEVNESEEARSKKTLENWKFQEQLAREESLKRKQNMQEMQLEVIESEKPLETSKDRRVEPISSETQEAEQQEVYNEPNVIDLGAISAHSNSNPFRTMVNESQQKEQIEIANTTENLSLTQSVTAVSRNSYQTSPQKGQVTVKHKKKAAKKKKVSLDDFILLKVLGKGNFGKVLLSKSKNTGYLCAIKVLKKDHIIQNHDIESARAEKKVFLLATTAKHPFLTNLYCSFQTENRIYFAMEFIGGGDLMWHVQNQRLSVRRAKFYAAEVLLALKYFHDNGVIYRDLKLENILLTPEGHIKIADYGLCKDEMWHGCKTSTFCGTPEFMAPEILKEQEYTKSVDWWAFGVLLYQMLLCQSPFSGDDEDEVFNAILTDEPLYPIDMAGDIVQIFQGLLTKDPVKRLGAGPRDASEVMEEPFFSNINFDDILHLRVRPPYVPEIKSPEDTSFFEQEFTSAPPTLTPLPSVLDTKQQEEFRGFSFMPDDLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XCC5</accession>
    <name>A0A8J2XCC5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Urea amidolyase</fullName>
            <ecNumber evidence="13">3.5.1.54</ecNumber>
            <ecNumber evidence="14">6.3.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">BN860_00188g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>urea + hydrogencarbonate + ATP = urea-1-carboxylate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20896"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15832"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>urea-1-carboxylate + H2O + 3 H(+) = 2 NH4(+) + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:19029"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15832"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.1.54"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="12">Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (allophanate route): step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (allophanate route): step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.54" evidence="13"/>
    <dbReference type="EC" id="6.3.4.6" evidence="14"/>
    <dbReference type="EMBL" id="HG316461">
        <property type="protein sequence ID" value="CDF90670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="196847at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004039">
        <property type="term" value="F:allophanate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004847">
        <property type="term" value="F:urea carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000010">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.10:FF:000010">
        <property type="entry name" value="Urea amidolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053844">
        <property type="entry name" value="AH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014085">
        <property type="entry name" value="Allophanate_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050856">
        <property type="entry name" value="Biotin_carboxylase_complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003778">
        <property type="entry name" value="CT_A_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003833">
        <property type="entry name" value="CT_C_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014084">
        <property type="entry name" value="Urea_COase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02713">
        <property type="entry name" value="allophanate_hyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006043">
        <property type="entry name" value="PRK08186.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00724">
        <property type="entry name" value="urea_amlyse_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02712">
        <property type="entry name" value="urea_carbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866">
        <property type="entry name" value="CARBOXYLASE:PYRUVATE/ACETYL-COA/PROPIONYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866:SF128">
        <property type="entry name" value="UREA AMIDOLYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21986">
        <property type="entry name" value="AH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02626">
        <property type="entry name" value="CT_A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02682">
        <property type="entry name" value="CT_C_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00796">
        <property type="entry name" value="AHS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00797">
        <property type="entry name" value="AHS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160467">
        <property type="entry name" value="PH0987 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0092">Biotin</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="19">
        <location>
            <begin position="629"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="18">
        <location>
            <begin position="748"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="17">
        <location>
            <begin position="1755"/>
            <end position="1833"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CDF90670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1833" mass="201824" checksum="8386775043D19D42" modified="2022-05-25" version="1">MVQDISELTRGWSVSDWIEFHCKSVPSASFDVLSKLLKSQKVAPEDPAWISLVSEAHLEHQWQILQSKSCKETLPLYGVPIAVKDNIDVYGVATTAACPAFSYQPSRDSKVVELLRDAGAVIIGKTNLDQFATGLVGCRSPYGKTPCAFSDKHVSGGSSAGSGSVVARGIVPLALGTDTAGSGRVPAALNNLIGLKPTKGVFSCQGVVPACKSLDCVSIFALNLSDAERAFKVMCQADERNDEYSRPYQTNPLQRYGSDVRVGIPNILPWFGETENPKLYASAVETLRSGGADVVTIDFEPLLELARCLYEGAWVAERYEATKDFLATNPPQDSLDPTVISIIKSGAKYSAADAFRFEYKRQGILRKVEALLATIDVLCVPTCPLNPTFDQVAKEPILVNSRQGTWTNFVNLADMAALAIPAGFRPDGLPTGITLIGKKFTDYALLDLARRFFDKKYPDGTRNYGIFTEKHVGIKDDELTGPTLTPEQSIKLAVVGAHLKGLPLHWQLEKCNATYLSSPKTAKKYKLYALPKIGPILKPGLRRVGEEAGSQIQLELYSVPLDKFGTFMSMVPEPLGIGSVELESGKWVKSFICEESGYLAKGAVDITSFGGFKSYVNYLQTQETESRRPFKTVLVANRGEIAVRIIKTLKKLKITSVAVYSEPDKYAQHVLDADVAIPLHGVSAAETYLDMDKIIKAAKETNTEAIIPGYGFLSENADFSDRCAREGIVFVGPAGDAIRKLGLKHSAREIAQKAGVPLVPGSPLVSSAAEAKKIAAELEYPIMVKSTAGGGGIGLQKVDSEDQIERVFETVQHQGKAFFGDAGVFLERFVENARHVEIQMMGDGYGKAIALGERDCSLQRRNQKVVEETPAPNFSEKTRSRMRKAAENLGSLLKYKCAGTVEFIYDERRDDFYFLEVNARLQVEHPITEAVTGLDLVEWMLRIAADQPMDFDSSHINVTGAAIEVRLYAENPVKDFRPSPGQLTEVKFPEWARVDTWVTKGTRVSAEYDPTLAKIIVHGKDRNDAILKMNKALNETAVYGCVTNLDYLRSIVSSEMFKEAKVATKVLDSYDYKPCAFEILSPGAHTSIQDYPGRLGYWRIGVPPSGPMDSYSFRLANRIVGNNSRAPGIEITLTGPKIRFHTEAIIAITGGAAPCTVNGSEVQQFTPLTVKRGDELAIGKLTTGCRCYLAIRSGIDVPEYLGSRSTFTLGELGGYNGRVLKLGDVLFLNQPGLEASTLPGPEYAPESPPTELIPVITAEKEWTIGVTCGPHGSPDFFKEESAKQFFSDTWKVHYNSNRFGVRLVGPKPKWARLDGGEGGLHPSNAHDYVYSIGALNFTGDEPVIVTCDGPSLGGFVCQAVVAEAEMWKVGQVRPGDQIKFAPINYKVARELKESQDQAISTFSRTALKTLTPELELPTFENAILAELPKSSDFSPKVTYRQAGDRYILVEYGNNEMDFNLSYRINRLIALVDRHKTVGIVEMSQGVRSVLIEYDGYKISQKDLLKTLIAYENEIHFDNNWSVKSKIIKLPMAFEDSKTIACVTRYQETIRSTAPWLPNNVDFIADVNGITRDDVRSMLYSARFMVLGLGDVFLSSPCAVPLDPRQRFLGSKYNPSRTFTERGAVGLGGMYMCIYAASSPGGYQLVGRTIPIWDKLMLAPNAKHPWMLSPFDQIEFYPVSEKELDKFSEDCENGQFTVNIQDGVFDHRDYLKWVQENIESIEEFQTNQVGEKSAEFAKLIQEANTELEKSQTSKPEAQEDYPEGAEFVYSEYSGRFWKPIVKEGDQVRANEGLVIVEAMKTEMVVVAPADGKVLKILHENGDVIDAGDVVAVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2SZZ6</accession>
    <name>A0A8J2SZZ6_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">GPN-loop GTPase 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_13388g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Small GTPase required for proper nuclear import of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2SZZ6"/>
    <dbReference type="OrthoDB" id="5839at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17872">
        <property type="entry name" value="GPN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000552">
        <property type="entry name" value="GPN-loop GTPase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030228">
        <property type="entry name" value="Gpn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF7">
        <property type="entry name" value="GPN-LOOP GTPASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF87741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="271" mass="30804" checksum="5B2398D3C72F0B9F" modified="2022-05-25" version="1">MSRVGVMVLGPAGAGKSTFCNELISHMQTIGRRAHIVNLDPAAEPNKYEFTVDIRDLISLEDVMEELELGPNGALIYCFEYLLQNLDWLDEEIGDYNDEYLLFDCPGQIELYTHIPVLPNIVQHLQVSLNFSLCATYLLEAPFLVDTSKFFSGSLSAMSAMILLELPHINVLSKLDLIKDEYGKKKLKRYLNPDPLILIDEVNKDTNPRFHHLNQCIAQLVDDFGMVQFLPLEARNPDSINTILSYIDDVTQWAETVEPKEPNDQIEIDEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T1Q9</accession>
    <name>A0A8J2T1Q9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BN860_11342g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_11342g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T1Q9"/>
    <dbReference type="OrthoDB" id="497380at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012959">
        <property type="entry name" value="CPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040059">
        <property type="entry name" value="PUM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13389">
        <property type="entry name" value="PUMILIO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13389:SF0">
        <property type="entry name" value="PUMILIO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08144">
        <property type="entry name" value="CPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUM-HD" evidence="5">
        <location>
            <begin position="117"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="146"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="183"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="219"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="91"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF87651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="651" mass="75047" checksum="4E8B27F948F0B6ED" modified="2022-05-25" version="1">MASMVKRHNKNGKRILDAGKSTTKPAKKPRISVDSSEEDELIEAPSNFAPKFEDEVEEDELDQLDEEESDQLDDNDENEKIEQENDDDMERDGKHKEQRKLLRDRKQSRKSGTQVQQIKNLWEKLRIKSAPMPKAERQKLADQVWELAKDCISDLVLKHDASRVVQTLVKYSAKDRREQIVNALKGKFYLLATSAYGKYLLVKLLHYGTKNSRQAIIDELHGNLRKLMRHREGAYVVEDLYVLYATNEQKQQMIREFWGAEYAAFKNSHKGLTIEEVCESSVEKRTIIARNLVGTITASVEKGSTGFQILHAAMRELVHIANEKEISEMIELLHEQFAELVHTPEGSEVACNLISRANAKERKIIIRALRDHAEALIKNEHGNEVFITLLLCVDDTVLVYKSFGPSVKEHLKEFIVHKYGRRPFLYILVGLDGKYFNPHVIKNFDRYVEMSKATSKKDSLQRRLELLEKFAPLFLQTVLHHHSEILSENLGSQFIAELLVNDELYDQLKEKDRTVFEEVVDRIAVTFKGDITEADHPIHKSFSTRLLKSLIQGGKWNNKEKKFEALHKVPILGVSFAEKFYENIIDSSNLLDWIENPDSSFTIVALFESLQGRKESKQFFNDFKSIKNKIDSDESNKGANLLLRLVKENKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T267</accession>
    <name>A0A8J2T267_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dynein heavy chain, cytoplasmic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Dynein heavy chain, cytosolic</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN860_05468g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required to maintain uniform nuclear distribution in hyphae. May play an important role in the proper orientation of the mitotic spindle into the budding daughter cell yeast. Probably required for normal progression of the cell cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="447173at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000235">
        <property type="term" value="C:astral microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000741">
        <property type="term" value="P:karyogamy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902850">
        <property type="term" value="P:microtubule cytoskeleton organization involved in mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein 1 heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000996">
        <property type="entry name" value="Cytoplasmic dynein heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002357">
        <property type="entry name" value="Glutathione S-transferase class-mu 26 kDa isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054354">
        <property type="entry name" value="DYNC2H1-like_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF36">
        <property type="entry name" value="DYNEIN HEAVY CHAIN, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22597">
        <property type="entry name" value="DYN_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0415">Karyogamy</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="1765"/>
            <end position="1911"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="2391"/>
            <end position="2541"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="3007"/>
            <end position="3062"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="3216"/>
            <end position="3257"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="3555"/>
            <end position="3582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CDF87398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="4050" mass="467291" checksum="8F11B00127E3077D" modified="2022-05-25" version="1">MQGDYDSVFRESLELILQCLNSLPEFPKVQYDTIHDEVVQNFAEQLQLFLVGGDMRSYNSLFVLVNKSSWSVKLEVEVTKVSCDCLVFVIIKNNEALDYDIPMRNQLTVLTLPGDIDTEFFASVVNNGLYPIFTILIESNLLPFTAESVGTTKARFKELTSSFHNLKNSIPIPELSIHPRIKQIVNLGASRETYKQYLDTTALEDSNFLNVLQSMANQWHRTLRNLIDLQKNMKDHSALQEVRFWGNMESALTAAVSQLHSTEVQVTLEILSSAKRIHSPLEYEEDSGITGVLKQVKGYNQFLDSVPLQLLQTTTNLSELHESLKTLAVSFKKFRSSDYPVEKFIIFLQKVSQDVGEKILQVVPNAFDIEYSQFQIVNRDVINILHLWDEVLRDNNIQIREVIRRKQLNNLSFVSLDSYTKKIKTLWEKITAFRQKHALLSDALEAISYEGNANDLKFIYEPLNTISRTDNFENWSTARIMYNQRLSTLEGKFIRMFEQKLYNCQCSERMLATFLNFKPLMDFYPKLRSVMKTHQQTLIDLISEEISLIEKRLSKESHLCNVLELRNLSTTAAIVTQCKQVELHLKHILKQLEQLLGQDFKTLPHGMSLIKKCDNIMSKFNVEKYLSELYEKMLRGDVGALEAPLLKILQDQHGKYEIRVNFDFSIGTLFKDSRNLIYMGYKVPGNVIRIARKYKAIYPQAINLLEQVQTLMSVLNELPLRPFTGILLKSQIEQGWLLLNAGLSTLWCQLPSDITSDIDNESENTILVGLEKHLSLILNDFGKLEHIESRISHIFEVLLGNNINSKVTIQYVLKMQEMINELSEVGISDQSDFVMSLNNSLKVVLKKKIKWFLHNLALDKRTVLITLDKGSIKSVPELHQMKSEWLKEIEDKVLSFEYLPQLCYLNPAQDFSVLHNYVQDILPLAYSNISEMYQEAVHYLNLWKKNERLWILEEETVKELIKCDMEKCVRLLTDILKVRPTSSFFEENPRLSIILTFEYGQVLSKILSKYDSWVKFLSDQLMALYMRNSSEFEKELRKARLFLEQNRFVVSSLKSVTDLVKTIDQVKEGRNDREFFLNTFSRSAELFRILHIKIPPDFIFVEQLESELNALSECSMRKEALLGKNRNIILTKLESEAQRIDNLAVSLLESWKEKKPMAIKWTPMEAMASINTFEESFISLKCDMQLISESARIMLIPIVIKDRFETFYEELLALKKVWTDLEGFWTRVNVLLGQNWDQVDLDFHLKEFKQIEHEMDLVPFKVQQYTIFSELLGSVQGVLNSADSLHLLKDPSLKLRHWQHLYKKAGKTIAQKIPDVSLQAIISIQSALNETIIKDLVQKAKKEFVLEEALNKIKDFWTDSQYSTFDHCSGLKLVREWGPLRQACIDDLDELHSMKNSIYYKHFEPECIELENKLTDLSIIQLEWMDAQFYWIDLYRVVGKNKEMQTLLPLESSKFQRLTSDLKMILSKIFSLNNALDAIHIAEGAAIFRRIVESLKLIKSSLNDFLEKKRQLFPKFYFLGNDDLLKLIGAGEDLERVSIYLPKMYGSIAGLKSKDGAIKGVVSTEREDFTLINEVPINSLRCHEWLTLLDKEIRNSISENVSRCLSRLEKGATFKSLLDSYIFQVLLLSWQIFWTSEINECFNKAKFSEVLTRMEEETGEVVSELNKRNTSLEKKKIRSLLVECLHYIDVIQKLNKTSLPKARLIWFNTQKFYYDDNNNIAKRVRVSLAGRINFYGFVYIGVPERLIYTPTLQKGYTALTEALYQKYGGCLFGPAGTGKTETVKSLGQNLGRLVVVFNCDDSYDFQSMARLMMGISQVGAWGCFDEFNRLSENIMSAVSSHVELIQNGLSTEKKSINLLGDILPLHEDTALFITLNPNYAGRSKLPENLKKKFREFSVNVSDNEIIAEIILKILGFEHSKALSRKVVNLFSTLTSECSNQKHYDFGLRTLKKTLKNCSELASQLPCKKEELILVESLCQIIMPTLIKPDEEKFMLCANEIFSFDNLDVQDESFVTSLSKICETDHLNPTDEFKKKCMQLYRIQKNQQALILVGEAAIGKTRVWKAVLAAIKMTEKVNTVVYRIATKTLKKEEIYGSINRATLEWKDGVFTSIVRQVLNDATETLQNTRVWVIFDSDMDPEYTETLNSALDDNKLITLPTGERLEIPENMRLIFETHSLDHTTPATITRCGLVLFTEGVRSAYEYLIMRLTHSFLALESQSPLQSPIFRKYKSAVFRFFSPTTTQLLFDEAKKVDHIMGFDYSRVIFTLTTLIIQNLQRYSCNLLQVPDHTFNTFFYSKMRQIIKFSFVGDTKMSDQVKFINSIFALFDEVADLNIISDDIEFLPETLEKINMDILVPQILLKPLDIMKPNIVIPTIETIKQDLLVFELLNARKSIILCGPPGSGKTMILNNALKRSADFELIGLNFSKDTNIAHIMKSLGRHTCYTSGSKGLMLRPKSPTKDIVLFCDEVNLPQPDSYGTQSTILFLREMIEKKGFWKADENKWVNLERIHVVGACNPPTDPGRIPMNPRFTRHVSILFVDYPIKSSLKYIYETFYRAVFQLIPQFKDFSVQFADASINLYEKFKNKIATNCKTHYLISPRELTRLTRGFYTSVINSLQHSMQSLIKMWIYEAWRVFADKLIHQAEKKQFQELLQEIVGEFFPNQVHYDFASSSLLFSSWLSLDYKEVTKQELSTFVQQRFQVFCEEEVETSFIIYDEMLSHLLRIDRVLRQVQGHAMLIGPCRTGKTTMTKFTAWLNGISIVQPNVHANFTISDFEEFLRGVLLSCSIEDKKLCLIIDESNILEPSFLERMNTLLANSDIPDLFQEENYDVLINSLKSRILSLGLLIDTEQEMYQWFIQQISKNLHVIFTIGDPYNDTLPSMTTSPALFNRCAINWMGNWSLRIMRQIASSIIQPMPLDIVTRDQIPLKNAGQVDEKVPDVVVNVFIIFHQKYLDHYQPKEASPGTFLDALKSFEVIFNQKYKELEEKQRFVTVGLDKLDESVLKVKELNKLFSEKQEQLKYKENEAKKTLDKMLHEQNEAERKQEAVVEIKEILATHQEESKRRRKEVMDELDAFAPVLNEAQHGVKNIKKQQLTEIRSMINPPSAVKVTMEAVCAILGQKISSWREIQNFIRKDEFIFDIVNFDTETMLSTETKSFIEQEYFSKPDFTYKVVNRASKACGPLYQWVFAQLKYGEILSKAEPLKKEAQTIEDEALQSNARLLAAEDMIAELEESIENSKQNYRHLIRDIELIKTEMLGVQTKLERSTTLISNLTSERERWAKSTHLFRKASIELTGNCIISALYSNYCGRLVEKQRKQMFQILKSLLVEYSIKFDTNYQFVTHNVEIEKRFEWAALGLPNEEFFLENFSMVLTSTNVPYILDPDPHIVSVVSRYYGKRFTLTSFLEPGFVKKLENGVRFGGVVLIQDAEYFDPIVNKLVSREYKAAGGRQTVQIGDHEVDLSLDFRLILYSGDIEANIPKFVEARVRLINFSMNKGSLEMKTLRIALFKEAPKMNQESNELMKLNGEYKIMLKTLETRLLEELNDSKGNILENDELIQTLERLKLESSEIENKLKETENFITKVNHFTDTYAPLGLHATILYAVLEQMAQVHWFYDLAMWQFIECLERIFGKTASTCDNRIEELVWNLYQETFLCFSSYMVKNHREAFAVVLFLTYYSYHRNAIFREKAVAILIYIQNNDVAEDINRIKVNCPPELLQAVEAIQDQSFKVALQQFQYPFQQQATIEEFAIHRKGQCILLGSNSDTDASFKVLQLANAHQQNLSVISLGSAESTHYAEQEIARSSTEGGWILLQNIQFSLSWTSSVLSRKMEQISKSTTMFMTCNLLGQELPSPLLQQSYKIAYEGLPGILDTVCDLYFNTSTQCRWKFLLCWFHSILLNRSLLAPIGFSKKYEFNDCDFACALQFLADIPDDNDARGKICYILTNIIYGGKVDADADHSIIQEICNKIFGEVESAVNKTPLKYQIVQGLMLPEATDHNTLSEFLRNLQEPEDARAKWLGLPDEAIKRYETIQAQKVASQALLLLEGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2D0</accession>
    <name>A0A8J2T2D0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BN860_14972g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_14972g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2D0"/>
    <dbReference type="OrthoDB" id="27218at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051170">
        <property type="term" value="P:import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000490">
        <property type="entry name" value="CRM1p Major karyopherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041123">
        <property type="entry name" value="CRM1_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041235">
        <property type="entry name" value="Exp1_repeat_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014877">
        <property type="entry name" value="XPO1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040485">
        <property type="entry name" value="XPO1_repeat_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF2">
        <property type="entry name" value="EXPORTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08767">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18777">
        <property type="entry name" value="CRM1_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18784">
        <property type="entry name" value="CRM1_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18787">
        <property type="entry name" value="CRM1_repeat_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01102">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="6">
        <location>
            <begin position="34"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF87809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1089" mass="124697" checksum="7096F4F0FB6332A8" modified="2022-05-25" version="1">MDSILDFSKDLDITVLDQVVDTFYKGSGVQQKQAQEVLTKFQEHPDAWQRADKILQFSQNPQTKFIGLTILDRLITRKWKLLPEEQRVGIRNFVVGMVITLCQDDMLFHSQKNLINKCDLTLVQILKQEWPQNWPDFIPELIGSSASSVNVCENNMVILKLLSEEVFDFSAEQMTQAKALHLKTSMSKEFEQIFKLCYQVLEQGSSPSLVVAALESLLRYLHWIPYRYIYESNILALLSTKFLMSSETRAVTLKCLTEVSSLEIPADNAPIKAQTVLYFQNTVQQIASGVVPITADLRATYSTASGTDQSFLQDFAMFLTTYLTHHRELLEDASAEDPNLKALLLNSHQYLIQLSKIEERELFKTTLDYWNNLVANLFQEVQRLPVNELNPLMQLSMGSHAINSNSGALNPEFLKRFPLKKHIYDDICSQLRWVIIENMVRPEEVLVVENDEGEIVREFVKESDTIQLYKSEREVLVYLTHLDVIDTEEIMISKLARQIDGSEWSWHNINTLCWAIGSISGTMNEETEKRFVVTVIKDLLALTEKKRGKDNKAVVASNIMYVVGQYPRFLKAHWNFLRTVVLKLFEFMHETHEGVQDMACDTFIKIVQKCKYHFVIQQSRETEPFIQTIIKDIQETTADLQPQQVHTFYRACGLIVCEERNEAERVRLLSDLMQLPNMAWDAIVQQSSANPDLLLDPETVKIIANIIKTNVGVCASMGPDFYSQLGHIYYNMLQLYRAVSSMISVQVAKDGLIATKTPKVRGLRTIKKEILKLVEIYISKSKNLDEVVKVLVEPLLNAVLEDYMNNVPDARDAEVLNCMTTVVHKVGHMIPQGVILILQSVFECTLDMINNDFTEYPEHRVEFYRLLRAINEKSFGALLELQPAAFKLFVDTICWAFKHNDRDVEVNGLQIALDLLKNIEQLNNTPFASAFYSNFYFIFVSETFYVLTDSDHKSGFSKQSLLLMRLIALVGDNKISVPIYQEGSAPQGTSNHVYLNEYLANMLANAFPHLVKEQIVNFLTALISQYKDPAKFNGTLRDFLVQIKEFGGDPTDYLFAEDKERELSEQHRIDRERAAKVGGLLKPSELDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2M3</accession>
    <name>A0A8J2T2M3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BN860_16798g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_16798g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2M3"/>
    <dbReference type="OrthoDB" id="28644at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000002">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005998">
        <property type="entry name" value="Ribosomal_uL30_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012988">
        <property type="entry name" value="Ribosomal_uL30_N_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01310">
        <property type="entry name" value="uL30_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08079">
        <property type="entry name" value="Ribosomal_L30_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30 N-terminal eukaryotes" evidence="6">
        <location>
            <begin position="8"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="5">
        <location>
            <begin position="84"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF87890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="244" mass="27835" checksum="6F97099A5A1AF8B0" modified="2022-05-25" version="1">MVAEKVLVPESQLKKSKAQQKSAEQIAAEKKARQAANKEKRAVILQRNAQYQEEYEAAERAVIDAKREAKASGSYYVESQPKLVFVVRIKGINAMAPKQRKVLQLMRLNQINNGVFVKMTKATSELLKIIEPYIAYGYPSYSTIRQLVYKRGYGKINKQRIALSDNHIIEEHLGKYGILSIDDLIHEIYTVGPHFKQANNFLWPFKLSNPNGGWGVPRKFKHFIQGGSLGNREQFINKLVKSMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2N8</accession>
    <name>A0A8J2T2N8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase</fullName>
            <ecNumber evidence="4">4.1.3.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">tRNA wybutosine-synthesizing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">BN860_17150g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TYW1 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="4"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2N8"/>
    <dbReference type="OrthoDB" id="271553at2759"/>
    <dbReference type="UniPathway" id="UPA00375"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000196">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01071">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="17">
        <location>
            <begin position="223"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="18">
        <location>
            <begin position="472"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="177"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CDF87906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="834" mass="94216" checksum="86311914B772D078" modified="2022-05-25" version="1">MTFFFPFSLQKFRAHRTRRHKSQDFVISLHFFNRLISEEFIRLALSLMIRLNGATAAGTFLLTMVYLWLGGRVTIALAIIVSYAILHKEYNVSNEQGQEKTPAKTEKKEKKHTCCGGKGGANCCSSKAGGAGGSGHACRCSSKNKAQEKKEEAKEEKKGLEAGSFPVAVDFTQAFKPQKKKSTKQPKVFSKPMPTKKKTASRDDDFKKSAMTVSNGTLLNSQIYVFYCTLQGTAERYANRVMAQLCTMRDLKQRPTLINLDEVTDLDDYFVSVPSENSLYILVLPSYDTDCPLDFFLQTLEENFNDFRIDKYPLRKLVGYSILGIGDTETWPEKFCYQAKKTDHWIARLGGRRIFPIGEVCVKTGGEPKVNEWIDLFCETMKDDEPILYEYDESVEDDGTENDEMVDLEDMGNAEGNNSTVIKQMVAKDSPTYKTLTKQGYSVVGSHSGVKICRWTKSDLRGRGSCYKHSLFNIVSSRCMELTPSLACSSKCVFCWRHGSNPVSKNWRWEVDEPDYILENALKSHYSKIKQMRGVPGVVAERFAKAFQVRHCALSLVGEPIMYPYINRFIGLLHEKGISSFLVCNAQHPEALANLCRVTQLYVSIDASTKGELKKIDRPLYKDFWERMLQCLDILNTTQSHQRTVFRLTLVKGFNMGEVMSYADLVQRGKPCFIEVKGATFAGSSDGNGNPLTMQNIPFYEEIKTFVKKFAAELQRRGLGYDVSAEHAHSNCVLIASTKFKMNDEWYTHIDFDKFFDLLASGQDFTPLDYVAKTPEWALFGNGGFAPGDTRFYRKGKKKIPETEKLSSTYSAAFVNSTQQEPVRGETAASFEIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2Q0</accession>
    <name>A0A8J2T2Q0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">BN860_14004g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_14004g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2Q0"/>
    <dbReference type="OrthoDB" id="416217at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070452">
        <property type="term" value="P:positive regulation of ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900436">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms in response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000911">
        <property type="term" value="P:positive regulation of sterol import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000022">
        <property type="entry name" value="Upc2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053157">
        <property type="entry name" value="Sterol_Uptake_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784:SF5">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="11">
        <location>
            <begin position="49"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="111"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="181"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="284"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="384"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="118"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="284"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="402"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF87766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="818" mass="89853" checksum="FA489FBEBB7AC79B" modified="2022-05-25" version="1">MNSAVKDRMERKSSLRKREKVVELIEIDGKKVSITSTGKRKFHNKSKNGCDNCKRRRVKCDEGKPLCQKCVNMKLECVYTPRQPKKKKESTVVKYVTRKAVTEGEGKLAAHGDAAQYSDDSSCNSSRSASRLPHQSPDDIAQADLQERKLFNGGAHGKAPAGTAAQQQQLQQQLLNARNMGSPDKMTAATPSSRGVLDGLLLPPLGSESSHLTQQQQQQQLQQQLLLLPQLMTLSNSEKYAAFEMPNSPPLQPAGMNITNSIGSIFSPSRFQLGQQQQQQQQQQQQQQQQQQPQQPQQQQQQQRQQQIRQSQSPQQQRTQQTKGTNLASNAISFQAESLAQLNKASMNLRAMGMFPTAGIGGVTYDFQELLGIKYPGNSQATKASNAEEALANMQQQQEQATKGEDGEAHEVSRNDHENVAVHMLATPSLPNNPTISPAAPITPMTKSSIESAAAASPSVNAAVAGAENNLPRTERADIVQTQDQATFQGSSGTGCIASLLQLSREGNLNLIDLKLFHHYCTKVWPSITAAGISGREAWSTEIPELAFEYPFLMHSLLAFSATHLSRTEPGLEPYVSSHRLDALRLLRQAVLQISEDNTDALVASALILIMDSLANASNQSSANPNSANPNSMSPSAWIFHVKGAATILTAVWPLTEKSRFYNLISVDLSDLGDVINQESATISELVCFDESIADLYPVEIDSPYLITLAYLDKLHREKNQSDFILRVFAFPALLDKTFLALLMTGDPGAMRIMRSYYKLLRGFTAEMKDKIWFLEGVSQVLPQDVDEYSGGGGMHMMLDFLGGGLPSMTTTNLSEFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2U5</accession>
    <name>A0A8J2T2U5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">BN860_06172g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_06172g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2U5"/>
    <dbReference type="OrthoDB" id="16516at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031251">
        <property type="term" value="C:PAN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06143">
        <property type="entry name" value="PAN2_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000028">
        <property type="entry name" value="PAN2-PAN3 deadenylation complex catalytic subunit PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050785">
        <property type="entry name" value="PAN2-PAN3_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048841">
        <property type="entry name" value="PAN2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028881">
        <property type="entry name" value="PAN2_UCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15728">
        <property type="entry name" value="DEADENYLATION COMPLEX CATALYTIC SUBUNIT PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15728:SF0">
        <property type="entry name" value="PAN2-PAN3 DEADENYLATION COMPLEX CATALYTIC SUBUNIT PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20770">
        <property type="entry name" value="PAN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13423">
        <property type="entry name" value="UCH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="USP" evidence="10">
        <location>
            <begin position="492"/>
            <end position="834"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF88272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1100" mass="124612" checksum="5C940B33F9D1CBB1" modified="2022-05-25" version="1">MNNWKPSLTDFLDLTEHLKKPFFRHDNREKEASKVVFDGNADLIWAGDSYARVSSYDSTLSLYCRYTANIGGLPVNDILSHREGILSLNEDSLHFASRRGVTQMNLTSVDIAAFSGLRSMCFGGGEYANDVYCAGVNPSSGIIRIDLTKGCQISIINYPSKVAHMCSNNKIITVGKQSGGVDILDPRSNSIIRSFVAHSGTISAMDSRDYTLVTVGKSKRFNNMYADPFVNVYDLRTMQQLPPISFSKGTTMGAGGADFVQLHSLLPTVMVVGSASGSFDFVDLANPTLRTQYIHPCQSIKSMTLSPNGDYLAFTDVNNTLTTWNRSNNSHNFTNTPNVLEYPDFVNDGVLSRPTEVDNYDYPLSSVGLPYYNQQLISAWPNSIFRSPGTIPVPIDEHMPISRPKSKSHPGSLPFYKYEKSKYGRRNALEPYVCLKEIRKKTVSDYSSDDILRYKPSEEGEIPRAFMKLPLTHGRYGSDNFDFQAFNNTPYSTLDTDVDNVYTNSLLQQYRFVPELFNFVVGSLKDENFSSNSLLTELGYLYDMLDRSKGKVCRSANFQSSLNSIEEARAQGLTTVPSNDLTFLERLDIEGESSSLSSPEKLRKSLIQKFNIFLLRRLVEEESNRVPSTAILKDLFGLQLDSVIATSCGHQEVIQSVEPTLTVLSPARNSAKYANKKLNNQSILPYIESSMKRVKYTTTVCAKCNKPEFTTYERTIENLPPMLAFDILLSESEWAVVKSVKNWLSKEFFASMTKEKAILRSNPLELRGTGPIFKYELNGYIAKISDRDGESRLVTYVRKYEPQERSFKWYMFNGYLAVEIDEEEALDVSYWWKVPETVIYCDAEEIRKPFFPVDTYRINYDVLYCDHFANGFRENAKTQYELLCRDEAPKPGTLVAIDAEFVVLSEELSDIDCNGIKTIVKPKKTALARLSVLRCEEGSQFGIPFIDDYIFNKEHIENYLTRYSGISPGDLDLEKSSKPLVSREVTYRKVWLLMQLGCVFVGHGLSNDFKHININVPKEQIRDTAVYFLRGKRFLSLRYLAYALLDKNVQEGNHDSIEDAYTALILYKKYLDLKEKGILEHVLDTIYAEGRASNYRVPFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3E9</accession>
    <name>A0A8J2T3E9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_09890g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_09890g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3E9"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin family profile" evidence="2">
        <location>
            <begin position="406"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF88422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="778" mass="90639" checksum="38C6E824AC635644" modified="2022-05-25" version="1">MMESTTFFKDHEVIYNSLINRLEQLFSKAYDICHKGAHPEDEMASEVSADVFEYNRESKQIMRTLTQFKVYAPIESDDHHGCKRFQLINQNKVISIIWNMLRDKLKDLTNDYVGNTLRLMGDLSLNTNLQSLFIFWDRMLITYNDALMIISPLLGYLTENYPLITKEIPRWSSISDYSHKMLIEMFKIQLGSNFVTFLETSLDYFRFQKEPSLSILEFESPLALMYKYNIAVNQDSTQDVKDFYFKYLSKYVQNELNIPLDIHYLKTLINNLRNNSLHTGAISPSLIEGANDIFLQHTVLQADTAAHIIDILKTNLPLRFKSRPHSFDVLLENFQWIITAFETRNRIHELEDILRRVVKKSLQRAYHDPEKLFSEVCKFVLLADKNTTFVVIPQTEMVKILGGSLNAMELYTRLCETNIRRANRKPVENFVDYYTNSAVVFYAPILLHINSTILNLYSRSLFRRAIMQGVNPVIKSLTDTRYLESNMLKFFKSQYGTTSEYHTLEATQEIIYKASSLQTSFEQHVSNHTNFMIPLVFEKKMVPIVFQESRNEDVNLPEELMEAWNEFIEFFKTTDKKAKMKKLHLVYHLQHCEVVTPYKLKNGKKLIFELTLYQTCLLSLFNEFKALSYREIMVKLRMTTSTLATVLTSFTEAGLLSAKGDIYALNKDYSPDKRRVKDGKLRIPLSKPAGLPRSMKQPNKVVESSQHREGHSSQWKQELVKACIVRSLKGERNGLNLTNLFKKVEEQIRGISMGEFKDALHKVLSEKFIASDNDLFVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3W7</accession>
    <name>A0A8J2T3W7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Deoxyhypusine synthase</fullName>
            <ecNumber evidence="6">2.5.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_18074g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]-deoxyhypusine + propane-1,3-diamine</text>
            <dbReference type="Rhea" id="RHEA:33299"/>
            <dbReference type="Rhea" id="RHEA-COMP:10143"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57484"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="EC" id="2.5.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.46" evidence="6"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3W7"/>
    <dbReference type="OrthoDB" id="294378at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.910.10:FF:000003">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00321">
        <property type="entry name" value="dhys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703:SF0">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF87947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="387" mass="42711" checksum="0246963F190E4F2D" modified="2022-05-25" version="1">MSKANEKLPDLLAEAVLKESVPVPDDFVKVQGIDYSKPEATDMRASDLVNAMKTMGFQASTLGEACEIVDRMRSWRGKHIDELEDHEKKGSFDDQGYQKTTIFLGYTSNLISSGLRETLRFLVQHKMVSAIVSSAGGIEEDLIKCLAPTYLGDFTLPGSKLRDQGLNRIGNLLVPNDNYCKFEEWIVPILDKMLEEQETYVAQHGNDCLDANSDTASPIWTPSKMIDRLGKEINDESSVLYWAHKNKIPVFCPSITDGSIGDMLFFHTFKASPKQLRVDIVSDIRKINSLSMESSKAGMIILGGGLIKHHIANACLMRNGADYAVYINTGQEFDGSDAGARPDEAISWGKIKPGARSVKVYCDATVVFPLVVAATFANGQPLKRYDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3Z7</accession>
    <name>A0A8J2T3Z7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_10814g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_10814g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3Z7"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000373">
        <property type="entry name" value="Vacuolar basic amino acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF198">
        <property type="entry name" value="AZOLE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="451"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="560"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="58"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF88461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="600" mass="64947" checksum="0B14F5320C2CA3C5" modified="2022-05-25" version="1">MSQQWQKMLQIDSRPSDDKDMLEKVSDEIHSEQCSVVYSPQGGGSKPEKPHGFILFACIGSLVLSLFLAALDIMIVVSLVEDVAKKFNGYSKTGWIFAGYSLPNALLALIWGRLACVIGFKTAMLLSIVIFEIGSLVSGVADSMNMLIGGRVIAGIGGSGLQSLCFVIGSQLVEESKRGIVIAYLGSAFGVASIAGPFLGGAFTTHATWRWCFYINLPIGGVAFFVFLMFYNPEGKGSLRNSLKATWTHLKLVKKLKTLHGWIAFANELAFRFDIIEFILCTAGTVLILLALTFGGNEYRWDSAAIIVMLVIGICLCIAALVYDFYLFPRLPTVKQNPQYSPLLVWPLIKKPGILTPTLGVFFVCAAYMCQLNYIVQFFQLIYNQSAWKASVRLIATMIPTILTVVISGVVNSKFGFVKPIAVLGGISAVVGGGLLTLLTNRSDSSDHIGLLILPGIAFGATIQSGLVSVQLQVDKTSPSAHADFVSVTTLNAFSKDLGFAFGGVICDTVFSVSVANKLHGNGNTWPIHSSNELISYRGEYFDGPRSKMGNLISNSIRNVFYMALGLSAIGFLFNLFTSNKMVDLSPHPKVDEESSISST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3Z8</accession>
    <name>A0A8J2T3Z8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxidation resistance protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_16732g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3Z8"/>
    <dbReference type="OrthoDB" id="26679at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF62">
        <property type="entry name" value="MUSTARD, ISOFORM V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="6">
        <location>
            <begin position="64"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF87887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="257" mass="29467" checksum="84C4A322745344A6" modified="2022-05-25" version="1">MKGTFNRWKSSWSQGTSDTNGTSQGNYTDETEGNSTRNTVSSNYDDQSLPPVRLNGYLPSTKKRLLTPEMCDEIRTLMPTRIQLYTDWHLLYSLEQHGSSLQSLYHNVAPKNNSPLRVGYVLVIRDNKHGIFGAYCNEPFHPTSDRRYYGNGECFLWKMEKVPNVRIGNEKNHQDHMQGDDEKWQFRGYPYTGINEFAIYCQSNFLSMGAGDGHYGLWCDDNLLRGVSNPSLTYGNDVLSREGKKFHIVALEVWRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T422</accession>
    <name>A0A8J2T422_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_17084g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_17084g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T422"/>
    <dbReference type="OrthoDB" id="21678at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904802">
        <property type="term" value="P:RITS complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013243">
        <property type="entry name" value="SCA7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037804">
        <property type="entry name" value="SGF73"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041251">
        <property type="entry name" value="Znf_C2H2_13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47805">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47805:SF1">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08313">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18508">
        <property type="entry name" value="zf_C2H2_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51505">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCA7" evidence="2">
        <location>
            <begin position="180"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="529"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="541"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="580" mass="64286" checksum="51DEE1316CED3073" modified="2022-05-25" version="1">MTGTGLEIKGIKQGIQNQYSDSISLSDEKASSESGWKELLSIVKNRPPEYNHINQATKEKFFNSNTHIIENPLEPSASFQYRVCNSCGKPLALNAIMEHLEDHCKGGGESSSVTHPKEMTDEDTESTASPRKHKENRDNKRPLTEEDSPSSEQDNKRFKEGSSTPQATPLKKQRKVKQRNPTEKHLIDFDKQCGVQLPEGGYCARSLTCKSHSMAAKRSVHGRTQPFDTLLAEYHKQHQTKIGAAAEKRAKQQEIQKHQKQIQKEHRKQSQQQKPSQRGKNKSNASAAGTGGNAKSGTSSGSGKHAGNDVNSNSSTSLSPEEETTQVLNGVSRSFLLPLETNVLSSVKRRSKYFRMREMFASAFSVKPGYTSPGYGAIHSRVGCVDLDRTTDYKFRIRTPQPINQMAPQGMTPQQLYKVQQNKIMQAQMMSMQNGSTNVHPPQEAEKRQQQMQAQSKVQQQYENHGATPQVVNNVQRTVPNQNAQSKGLTPQEIQLQHQKLRQQQLQQQRFEAAAFHLANATKLMQNSNGEAQESPTNYGTNSSSAANTPTSNQSIGSSVGGRLNVGVKNSMNGYGGRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T438</accession>
    <name>A0A8J2T438_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_12574g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_12574g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T438"/>
    <dbReference type="OrthoDB" id="5395343at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013948">
        <property type="entry name" value="DNA_replication_reg_Sld3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042511">
        <property type="entry name" value="Sld3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041393">
        <property type="entry name" value="Sld3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28067">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28067:SF1">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18523">
        <property type="entry name" value="Sld3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08639">
        <property type="entry name" value="Sld3_STD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sld3 N-terminal" evidence="3">
        <location>
            <begin position="6"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="DNA replication regulator Sld3 C-terminal" evidence="2">
        <location>
            <begin position="146"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="DNA replication regulator Sld3 C-terminal" evidence="2">
        <location>
            <begin position="243"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="551"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="554"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF88537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="652" mass="74059" checksum="B7C528778ED199E0" modified="2022-05-25" version="1">MATWRLLASFKLLPKSLSLDHEKVQVLAHDSILRVVLPESRANKTGLKHLLQGAEKYQLLERYGNLFWICWDVIGIDLQDVHDDFVGDEHSVNWMALKEWNQLNLKELLPLWKEDDSSHTVHETSKLHMKPPGGAASSQDVIRVDPRTYLETKYCDSLFNIRLPLAYFVKSNLSRLKNACNSNFNDGNAYLTIILKKLLSISEFDKRHANEGLLRVDLDGIQAERRKSCLAKCGIRISEGLQQEMSSLKELPAILKIREIKLQIIILLETIDHNKLDGNFENFETRYQCKLRRRSLNLTKRRALPMKSSLTKLRSDNNANNKAPIQLDYCEQLDLYLDKLCILDILLASEAISTENDNMDAILEHKKNILNKNKEASSMGFTNYVLIPYFAKKVPNTIRFTIQKLKGPSLRGRKNSERNITPQPPTNISENTKLDPPIDLGMNSSASSPQFTTNSSWCRNNSAMTTPKMSNIRTISNANVLLDSNPTLLKKPTFIARTKSDLTMNLMRKRQLSVTEFSSYDQKVPSNDQFEKSDNSTCSLRLSQQRSFRRVGKRKSERDITDTNHDLAGTSNSSETIQVMGTPLVKISNDTPRNRAKLQNIVESPMGQLPSMRTPSNNRISECTGSASAVRPEEICAANKAKKNIKRRLFAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T439</accession>
    <name>A0A8J2T439_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_17458g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_17458g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T439"/>
    <dbReference type="OrthoDB" id="4069860at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054413">
        <property type="entry name" value="LSO1/2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22048">
        <property type="entry name" value="LSO1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LSO1/LSO2" evidence="2">
        <location>
            <begin position="11"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="91" mass="10701" checksum="5A9AA40BFEF79F20" modified="2022-05-25" version="1">MGKTFRDSAVKKIEGRQKKKQQASAREQAELDRKEAEEQAKWEQGAKKPHAKKQEEEEKRQARIKAKKEREELAAQEEEEIKNEGKAKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T440</accession>
    <name>A0A8J2T440_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BN860_01442g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_01442g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T440"/>
    <dbReference type="OrthoDB" id="240216at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004092">
        <property type="term" value="F:carnitine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.20">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042572">
        <property type="entry name" value="Carn_acyl_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF48">
        <property type="entry name" value="CARNITINE O-ACETYLTRANSFERASE YAT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="8">
        <location>
            <begin position="24"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="204"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="837"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="838"/>
            <end position="856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF87218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="894" mass="99766" checksum="462D9FE13ABEA008" modified="2022-05-25" version="1">MPSVGNQPDEGAHTFAYESSLPKLPLPELSSTFEQLKQSLNPLYCAGGFYKGSTDPEQWDTFSKCIKEFLHSELAEKLQATLAEYNASSDCYLDNLHLDINNNSAREPEDDVLPRNPFLLLGDDIMPNVSQVERSAVLVHAALRFIAALRQEVLSPDMDGASANDDKDHPLAMSPYLNLFGTTRCPVFAPGEVEAFDLGKPFKESDLESSWSSDSSDDEGDESPEPGTVGDTFTRHGITIKRYPDSKHILVISKGQYYTVDVLDAKNNVLYNPSHLTTILDHILRDSSEDRAVRIDTALGSLTTHSFRNWKYSRKRLQRKFPRELRLIDSALFVLVLDQSDPCDEGEPTAGVDGLQILGGSEEGGMGTTSKDLKRFFYGTSIIDDNGHQVGSCISRWYDKLQMVVTKDAQASVIWDCFTCDGSTVLRFVSETYTESILRLAREVNLGDPKFSLWPSIGVSSVTSASNKKALELDPSIVTHKINWDFSDILNTHIHLSETKLADLISKYDMVRLSVPYGQRTANRWGVTSDSMIQVALQVAHYALYGRMAYGFEPVSMRSFKNSRSCFVNIQNDDMLEFCEEFISGSLDDSNKLNSFMQTCKKYTERVRRTKLGSGFEKHFNAMRFLFKFSEHYGFRLDAKDKELGQAVFENPLLAPLLAPELLAANCGNTATSIFAITPAQPRGFGIGYTIKDDHCDLTVTSQYRQGARLMFMLNWVLIEITRMWQKAAQGSKNRNKGYKVNPLVDKLYELDNALMHPQKRHNKVIKGYGFFDLQPHWDSTNTSAVNSASNSSLHLSSMGSSTKLHGISPVTYSSIPLSVPTESEKRDTGFEITQVVDGERGGTSDTKPDTSKDGATESSPSVCFNRRTNVIKSKFEIDFERGGVGKKVAPVFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T473</accession>
    <name>A0A8J2T473_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase</fullName>
            <ecNumber evidence="4 14">2.4.1.256</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_15214g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the third and last glucose residue from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide intermediate Glc(2)Man(9)GlcNAc(2)-PP-Dol to produce Glc(3)Man(9)GlcNAc(2)-PP-Dol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>an alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate = a alpha-D-Glc-(1-&gt;2)-alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29543"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="Rhea" id="RHEA-COMP:19512"/>
            <dbReference type="Rhea" id="RHEA-COMP:19522"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:132522"/>
            <dbReference type="ChEBI" id="CHEBI:132523"/>
            <dbReference type="EC" id="2.4.1.256"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:29544"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the ALG10 glucosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.256" evidence="4 14"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T473"/>
    <dbReference type="OrthoDB" id="4769at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106073">
        <property type="term" value="F:dolichyl pyrophosphate Glc2Man9GlcNAc2 alpha-1,2-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016900">
        <property type="entry name" value="Alg10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12989">
        <property type="entry name" value="ALPHA-1,2-GLUCOSYLTRANSFERASE ALG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12989:SF10">
        <property type="entry name" value="DOL-P-GLC:GLC(2)MAN(9)GLCNAC(2)-PP-DOL ALPHA-1,2-GLUCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04922">
        <property type="entry name" value="DIE2_ALG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028810">
        <property type="entry name" value="Alpha1_2_glucosyltferase_Alg10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="245"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="293"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="485"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR028810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF87820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="521" mass="60767" checksum="31CCE0312CA4ECFD" modified="2022-05-25" version="1">MGIKDVSKEEDNVIKGATQLIGPGVQRDLQTEVVVSFIMNVILYPLVLLYFMVTFYYVTTKITPHEFIDEKFHVAQTLNYVAGNWTSWDPKITTPPGLYILGWFSYRLGRLFTSWTSLTLLRMVNLLGGTIILPFTVLRPLFLFNAIGFWPVALMCFPLMAAFYYLYYTDVWSTILILQSLTCVLTLPFGINGSIWVSSLCAGLSCLFRQTNIIWTGFIMVVAVERRAIIQKQYNAHRFNNYLKLLIHSVDDFSTIVLPYAINFTMFVIYLIWNKSITLGDKSNHSAGFHLAQLFYCYLFIAFFSAPLWFSSSFLRLYRNRLFHSPVQYVVELLGIMLVIRYFTKVHPFILADNRHFTFYLFKRFIGNPSKMVKYIFMAPVYHFSTYVYFETLRPSEMAFESMEPVPFKDPIDLPIQLSHISWTALVICTFVTIVPSPLFEPRYYILPFLFWRLFLTCNAEPLIGEVKPAAPGEPPVLVSSTQRLALEFIWFMLINLFTFYIFKTRPIVWKTESFLQRIIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4D2</accession>
    <name>A0A8J2T4D2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">BN860_18096g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_18096g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. METL family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4D2"/>
    <dbReference type="OrthoDB" id="416496at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000371">
        <property type="entry name" value="Methyltransferase OMS1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050508">
        <property type="entry name" value="Methyltransf_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912:SF83">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF87948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="446" mass="51653" checksum="7423D6C94369F54A" modified="2022-05-25" version="1">MLIQAFKPISKATLAATRVGSLQKCLLRCQSTHTFVPKPNSRVRRIKTKEQLAQEKFEEQLSSDHWWVRWGAVARSEKFTKGMTKYLIGIYIVFLFFGGYYMKKLYRKDKELEHLQKKVSSGDANEYEQLRIKELRNKLRTRDRLKLEVYNKMKEEGRENFDGVRLDNNDENRLNKNILPSRDTTGFYDKKAEEYDAGVNFEEKAIFMGKRRKWLMRHCEGDVLEVACGTGRNIKYLDPSRLNSITFLDSSEKMMEITHKKFRDMFPRFKKAAFVVGKAENLVDLAQGTSIEDNDGLKTNNKDIGVIKKGSTKVKYDTILEAFGLCSHEDPVRALKNFAVLLKPGGRIVLLEHGRGQYDFVNKILDQRAEKRLQTWGCRWNLDIGEILDDSGLEIVEESRSHLGTTWCIVAKKKGDIKKKNEIGFFEKYFQIGTKREGTQSDGPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4D9</accession>
    <name>A0A8J2T4D9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">mRNA 3'-end-processing protein RNA14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_18492g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Nucleus and/or cytoplasm.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4D9"/>
    <dbReference type="OrthoDB" id="26282at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180010">
        <property type="term" value="P:co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045243">
        <property type="entry name" value="Rna14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008847">
        <property type="entry name" value="Suf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19980:SF0">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19980">
        <property type="entry name" value="RNA CLEAVAGE STIMULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05843">
        <property type="entry name" value="Suf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="2 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Suppressor of forked" evidence="6">
        <location>
            <begin position="31"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF87963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="651" mass="76347" checksum="EA8E57C2ADF865F4" modified="2022-05-25" version="1">MSSAATPELSLGDTSQSGLPEKKNVNEVESGIRQKIEVNPADILAYLELVQYYESNDAFAEAREVYKKLHEKFPAYSPLWTLQLKGELQRDEFEFVEKMLAQCLSGELANNDLPLWSTYLDYVRRRNNLITGGQEARAVVVKAFDLVMDKCAVFEPRSSQFWNDYLSFLEQWKPVNKWEEQQRLDMLRKLYKRMLSVPFDNIEKMWNRYTEWEQDVNSLTARKFIGELSSEYMKARSLYQEWSHMTKGLRRSYPVSLSTANKNNVPQAGEGMDPSQLQIWLSWIVWEKENKMSLSHDQLHARITYVYKQGVQCMLFAAEMWYDYAMYIQDVSERQQILYTAVLANSDSPSLTFKLAECYESDNKVDEVKRCFEGCTQVLQHKYQTMTNSRQDDMLIFKQRKKLTFVYCVYMNTMKRLSGLSAARAVFGKCRKLKRILTHDIYIENAYLEFQNQNDYKTACKVLELGLKYFQTDPVYVNKYLDFLILVNRDSQIKTLFETSVEHIQDLGGLKSIYKKMISYESKFGTLQNVYSLEKRFFERFPQENMIEILTDRYKIQDENMIKQLELNYLDDDDSAHDLADLTGSSLKRARDTQDKSYSNKRLKRGSAGVPEEIVNLLAILPKRQYFKVPLLDPQSLINYLNDQVELPSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4E6</accession>
    <name>A0A8J2T4E6_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Malate dehydrogenase</fullName>
            <ecNumber evidence="3 12">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_18844g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3 12"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4E6"/>
    <dbReference type="OrthoDB" id="4069699at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01337">
        <property type="entry name" value="MDH_glyoxysomal_mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000017">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000001">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
        <property type="entry name" value="Malate_DH_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01772">
        <property type="entry name" value="MDH_euk_gproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF73">
        <property type="entry name" value="MALATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="13">
        <location>
            <begin position="19"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="14">
        <location>
            <begin position="164"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="24"/>
            <end position="30"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="134"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF87978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="333" mass="35202" checksum="1F1BEB493F994790" modified="2022-05-25" version="1">MFTRIARRAFSTSKVNPYKVTILGAGGGIGQPLSLLLKLNHKVTDLRLYDLKGAPGVAADLSHIPTNSKVSGFTPDNEGLSKALKDADVVLIPAGVPRKPGMTRDDLFSINASIVRDLAKAAGDAAPNANILVISNPVNSTVPIVAETLKKKGVYNPHKLFGVTTLDSIRAARFISEVEGTDPTTEHVNVVGGHSGITIVPLISQTKYKTMDKTTREALIHRIQFGGDEVVKAKDGAGSATLSMAQAGATFANAVLSGLGGERDVIASSYVDSPLYKSEGVEFFASPVTLGPDGISKVHPVGDISSEEEQLLEKAKETLKKNITKGFAFIDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4P8</accession>
    <name>A0A8J2T4P8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S03-05094g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_05094g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the IRS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="10045710at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="F:transcription coactivator activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IRS4p EH domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EH" evidence="4">
        <location>
            <begin position="802"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="158"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="586"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="631"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="272"/>
            <end position="287"/>
        </location>
    </feature>
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        <location>
            <begin position="307"/>
            <end position="348"/>
        </location>
    </feature>
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        <location>
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            <end position="395"/>
        </location>
    </feature>
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        <location>
            <begin position="420"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="443"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="456"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="486"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="550"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="586"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="689"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="706"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="724"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00061579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF88925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="929" mass="104547" checksum="4370036D4D99854C" modified="2022-05-25" version="1">MIFHKNKSHPGTGENSEPSMSDSLRAAQIIFQKHSDAGQAVTPTTNTPPSHKRSTSAQSTPAKSAASAVRNRAVTPPPIGTSQNNLKGHATSPPSEHAHLAAALAHALMNPEAEEELSHSAPIEYDRLSPVVAKQKQPQLASHPISGQHLAHLYRAPSSRNSSNCNVSTSNNGSVTSLEELPKLDVSRDLSSMMKGTPTKQLPPSAPKQVHKSASEQPLQTPIIRTPIDSQKQQSLPSHRGPAEQIKQQLKESYRQPAEQAKQQVHTVNSPPRSPPRSPPRSPPRSPPMRRELSLPTQVSTKALPMQTPQQLQLQQNLQQQQQQQQQQKSHQQHQQQQHHQPQQQQNKQTEEPSSQPRPDFRAHPYSSPAEFSGLDAQMSAMSPATSNGPQSQPGGSHKPISRDLTRRIPPPRIYLGSSGDDETATDSVVENEGDLVSDRPILRARIKRRIRPPIDESSSSVISSDSPVSYGMENSSWDDRRLEDTDNYVEDPEEDDDYFDENNIIDDDDDDDEYDDYYDDEIDDEYNEKEYSLPPRGDGSSYDRYDFPETSSLPNLDNGSSTLAPTYSAAAFPGANLVPDRYKQGQPTRSYTQNAGRIPHQPNVTYQGTLPDLIPNHIRRTKMEKLRNKIFGSRSSKKKPHSPFDFSGDRTSVTSDPEGRPLVKTNQNMRFKTTMRKDPYEGSLGDNHGTEKGGDNDEYGRNDYDLEDSDEDSDSSIEVREEKHKHRGKAIRKKLKHTAAVVPYSHHLPHMDTRHTPKIFNEDKPWKSHNDVGFVTSQERKRYEGMWVSNRCTYLDLLPWWDAVLDGDSDINVQLPEDGLMLNLVVKDIWSRSNLSNELLSQIYDMVDTRGDGTLDRKSFVVGMWLVDQCLYGRKLPKEIDHKVWSSVDKYVVSTINSANLKQLDRSRKKLMKQELKNIKKEIKNVHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4U5</accession>
    <name>A0A8J2T4U5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ZYBA0S03-10352g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_10352g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4U5"/>
    <dbReference type="OrthoDB" id="62120at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF9">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035312949" evidence="7">
        <location>
            <begin position="30"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="510"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="8">
        <location>
            <begin position="108"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF89151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="528" mass="61034" checksum="5555D5C1FA115970" modified="2022-05-25" version="1">MLLRHTWRICCVFGSYLFWGCCCLAATESSNTLRDKFNDERPTVIKGITLGGWLLTEPYITPSLYESALKLAGNLGRNGTIVDEFTLCQVLGYEEAKSLLKAHFDSWITEDDFRQISEDGFNLVRLPVGYWAWKEDHERGQYIGNVTYPDPYVSDGLQLDCLEKALSWAKKYQLNVWIDLHGAPGSQNGFDNSGQRIFYDELGWLATEESIQLTKAIWQVMFDSYMKLEDDSTVVGIQIVNEPFSFRLNREMMVKSYYEAFEIFEAHHDSPPAIKFVIHDAFLEIGYWNSHFNPNYGELLEKYTNLTKGFDPQQVLVDHHRYDVFSDGQLRESQQQRIRNIINYGKSIHDELSYHPAVVGEWSGALTDCARWLNGVGIGARYDGTYYDTTDFHTDKDPIGSCISQSPIENWPEWYREEVRQFVEVQLATYSTQTSGYIFWNYKTENAVEWDYLALKKNDMFPQPLDNYTYFEPNGSVKSTKSPVFKPTTTDVHGTSHLWRKPYEHSRNPLWVLLVIIIAAVVAITCRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T537</accession>
    <name>A0A8J2T537_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_08922g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T537"/>
    <dbReference type="OrthoDB" id="10254310at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016342">
        <property type="entry name" value="AP_complex_bsu_1_2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002291">
        <property type="entry name" value="AP_complex_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="8">
        <location>
            <begin position="13"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="646"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="705" mass="80725" checksum="CB3D31CBBF1AFB14" modified="2022-05-25" version="1">MSDQRIFARYKASEIKAELQNFDVKKSRISATKRKVALRKIIANLKMGNYNEMALLFPEVLRFWKVEDDMEVKRICHEYVRALGAVKPQNAKDALSAVLDDLDSRNEEIQIMALTTLAAVTSLEYVNELFKAVCFIIARRSVAPNVLKTAILLLKQMDEVDHDRVSNILDSLYDIFDRQLASPTVQVAALHTLYSIHEKSSDLKPLRLPLETAMVMLDLLPKLNEWDKAMVLESLTIAAVPQTHSDVYGMIDLVLPQLQHANTYVALNAFKFIAYLLNYAEHVNEGLIKRFSNSIVSLLNKPPELEFLVLRNVILLLLSRGSPLLKLDVPYFFIEYNDPIYIKDTKLECLYLSADRETLPRILEELVQYATDIDIQMSRKAIRAIGNLAVKLDEESAYDCVNALLDLLEFGVDYVVQEIISVFRNILRKHSKRFESSVRELVKYTESVQEPESKNAMIWIITNYSYSLPNYLEYFQIFSSNLLEETLDVQFSILNSSVKFFVRDPNHETESLCMHSLKVCTEDINNPDLRDRAFMYWRLLSLAQSSKNNFLSDVSLKSLIDGTLPAIQLNTKLDPMVLEELELNIGSIASIYLKPVSQIFKNYRPKHLLASPILNQNKESLRIVNDSVPSLNFDAFDSVPEWETIDNSERSSTSSRKAKTMMNDYDKPAEKVNQLKGKRKSSSNSPSLTRKPSMLVRKLSMKKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T550</accession>
    <name>A0A8J2T550_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ZYBA0S03-11298g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_11298g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNR ribonuclease family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T550"/>
    <dbReference type="OrthoDB" id="372421at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.690:FF:000001">
        <property type="entry name" value="Cell wall biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.700:FF:000002">
        <property type="entry name" value="Cell wall biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041505">
        <property type="entry name" value="Dis3_CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041093">
        <property type="entry name" value="Dis3l2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF9">
        <property type="entry name" value="DIS3-LIKE EXONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17877">
        <property type="entry name" value="Dis3l2_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17849">
        <property type="entry name" value="OB_Dis3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNB" evidence="3">
        <location>
            <begin position="689"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="71"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="157"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="434"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="157"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="258"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="442"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="475"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="492"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF89191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1243" mass="136886" checksum="B6477A2D971E3DE8" modified="2022-05-25" version="1">MSYKNQDNNIYVPTTGGKNNPKHIHVAHRRSQSELTSLMIEQFTLQKQLEMVQQQQQQLLAQQHQLAQQTGQMLGQAGSNSSLLPPPPHYGSGGGVSGPNSSNGSGGYRSRSHSRNNSGYYDNTPGGGMSGNAHKKTGSQSSIYGHARRHSLGLNEAKKAAAEEQAKRNGETGSGVSVKVDDVSSSEASSNDGGEGGDAYGRPQSGFKFPSQSGHRRTNSNQAASFKFPAPPSNQKENDFDDFIPTASTHRRSRSKNNDYSAGINSNWRTQSQQPSPFHHRASNSRDFGNNNNSFGGLEPPPSFQAGHKHRSSNSSIHSFNSNNGNNPNARKSLFAPYLPQANIPELIQEGRLVAGILRVNKKNRSDAWVSTEGVLDADVYICGSKDRNRALEGDLVAVELLVVDDVWDSKKEKEEKKRRKDASIQHDIISLSSNDDYHNDASATASTTSNNITSNSASGSGNGGSVGDEASAKSGANATTDGEEQSPSKTGVKRRGSLKQRPTQKKNDDVEVEGQSLLLVEEEEINDDFKPLYAGHVIAVLDRIPGQLFSGTLGLLRPSQQAHNDNNNNNNNKQAQSPKIVWFKPTDKKVPLIAIPTELAPKDFVENADKYVDKLFVASIKRWPITSLHPFGVLVSELGEINDPNTEIDSILRDNNFLSNEYQDPKDPSTEKSISQALPVSEEDFAARRQFADEVVALEDRPHLSDFAIHIKRSAETIELGCHVVDVTAHIEEGSSLDRRARKRSTAVFMPQKVVDLLPQALNKSLALQEGKKSSAISVLYTLDAKTLKLKYTWVGESVIVPSAVISVEEVSKTLESGDPTGLVKLLEQLATAFYRERIKSPSAKLLPTLPLFKSLDDEKVNVDLNILNSSIGSVVLDEIRTKLDSTVAERIYTRLGGLAFLRRQVPPVHTKMASFKKKVQKLGYNLDIASPDALIQSILQIENDDVRSGVEILLFKTMSRARYFIAGKVDPDQYGHYALNLPLYTHFSAPLRRYADHVVHRQLKSVIKGVPYSEDSDSLKITSEYCNFKKDCAAQAQEQAVHLLLCKTINDMGNATGQLLTMATVLQVYESSFDVFIPEFGIEKRVHGDQLPLIKAEFDPVHRVLELHWQPGVDSAIFVPADEKNPKSYRTSIKNKFKSAAMEIATSEFEKADSGDSTVSEKLSEELSKLHIKVPQLRLPERSGKDAIESFIACTSTRRENDNYIQEIQELQQVPILLRAEVGMALPCLSVRALNPFVKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T561</accession>
    <name>A0A8J2T561_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_10374g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_10374g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T561"/>
    <dbReference type="OrthoDB" id="3993678at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16148:SF14">
        <property type="entry name" value="MYND-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16148">
        <property type="entry name" value="NF-KAPPA-B-REPRESSING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="17"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="351"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="486"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="631"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="881"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="351"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="486"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="511"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="549"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="702"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="728"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="743"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="761"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF88442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="945" mass="104375" checksum="F08F44D1D773FA57" modified="2022-05-25" version="1">MAKSQGALRSELLSNDYGNINVTEEDRLPGTGPSQGMQQGMPHGMPHGMPHGMPHGMPPQAAPNGMHPQGIPPQGMHNGIHPQGIPNGMVPQGVPFRPNGLPNGVPNGVPNGVPNGVPNGIPNGLQMHGGMPGGMPGGMPNAMYHGASMGMPNAMHSGMPRGIQSSTSSPQRRAYSLNNNSISNFFRQKPRMPHRSKRSSGIDGGDDDEEVLIEDDDTLTFNDIRAMGKGEKYGFGADTAPIIPTLITKNHSNMNNTEYRKAMTAQRKQAMSAMARQRNQGAPAAGNDPRAMSLQSYGMGNPFNPIPQAQQQIYPAQNMGTPRSMSMMTTQSRPPAGYMRQQQQKMFAARNNNNYNNTNNNNNNNNENGPHNVKLNPISTSHTLDPGPMTPDHEHAANITSPLKNEVASASPVSPPPNAAHLNVLQLSQPQQDEFRERERMLAEREKELREKEQLVKQREAEVQLELKQKEEELERERLRRDQEMFQREEQREQDQPRESQFHSQKNSERQSQSQQQQLQDLSSCDTDDLDLKPSSTLERGLDGMSLNDRQDSLKDGSIGHERNRESAMSYSAKRESTVSFGRNRESTVSFGPQNRVSTSTFMSVVSDSPEKKHKQSTGLYKLENSGNNEFFTAEEFPGDSGVASEGTGAINTSVDQRASHSQQRHSSIVEASGNGTSNYRSKKILSKISSKSLSSKENEPGATQTSSGDSPSNVSMGSFPSSNEDTFDFKQLPKERKEAVNNGASNKSVKPSSTRQLLKRTSEPIKEESSLEDSIDGFVFDNTSGQPYQPVFAQKNELAQLNNRFQTIVIPAEQLNIITENKELMSEISIVSAELADSIKRECMLEERLRNGSSGDSSDSNESLFLLQFETELRKKSSKVVELLKQLNEQRLKRLIAEEQLLLSEHGAKPSSIDLVHKISQLEAQLESKESEIQRLRESQKSAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T569</accession>
    <name>A0A8J2T569_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleolar complex-associated protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_08834g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for synthesis of 60S ribosomal subunits and the transport of pre-ribosomes from the nucleoplasm to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
            <location evidence="1 5">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T569"/>
    <dbReference type="OrthoDB" id="10263597at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011501">
        <property type="entry name" value="Noc3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016903">
        <property type="entry name" value="Nucleolar_cplx-assoc_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14428">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14428:SF5">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07540">
        <property type="entry name" value="NOC3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028977">
        <property type="entry name" value="Nucleolar_complex_p3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Nucleolar complex-associated protein 3 N-terminal" evidence="8">
        <location>
            <begin position="143"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="CCAAT-binding factor" evidence="7">
        <location>
            <begin position="480"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="30"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="89"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="372"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="89"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="126"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="372"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="386"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR028977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="670" mass="76432" checksum="6D8EC5D5FB4F70BF" modified="2022-05-25" version="1">MGRRKRSQSNIKDATAKRRKYEDVLLAQGSFGADGSSTADKDQYENAARKITQESWDNEEQDYEKEGRRLKGSEENLVEGLPIKVNGKIERKMRKQEPKEIENDTNANDTLEEAINRESLSKPEQEPESEEEEEPDTEERIVQLKEEIADLVERILEDPEENVSALFRLGKMASSKNPNTCKFSMLALVPVFRSIIPGYRIRPLTDIEKREKVSKEVGRLRYFEQSLVTVYKNYVDLLTDLARVPNNESLIKVQLGALAVHAANEIISNASHFNFRETVFTIILRRLCKPNLKADSMSEECVKTVEGLFRDDEDGSISLEVVRILCRTIKARKYNVEETVVNLLLSLDVLYDYDPNTKNAEDMHTVTIKKKDRVHLSKKQRKSRKEMKQIEEEMRKAEQSVSAEEREKNQSEILKLVFSLYLNILKIQSPKLVGSVLEGLVKFGNMSNFELLGDFLEVMKELIRDADLDNMSSAEVRKVLLCIVSSFSIVSNHNQMKFNIDLSSFVDALYAVLPAISLDANIELSHKSMRLADPLDSQIAKPSVNVSTKAELLLKALDHIFFRSKSGTRQRAAAFTKRIYMCMDHTPEKTSIALLKFLDKLMNKYQEIGGLYSTEDRIGNGDFIMNADNPARCNAESAILWENTLLVAHYCPEVVKGIRFLASRCKESFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T573</accession>
    <name>A0A8J2T573_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_11056g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_11056g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T573"/>
    <dbReference type="OrthoDB" id="3981131at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF88472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="337" mass="37401" checksum="7408C57002B511B4" modified="2022-05-25" version="1">MVDTTTHDSTTTVKDTSTSSQSSLANENETETSNEELQRAIAGGVKLIRMLEESSSDGKGQQSSRKLLGAILETDYDKEMPHKDITVVVNSLVEVLNSYVRQNQQLKLKNMILTSNSGDLQSRYEVEENLKKQQFERMKCQLFLEKENLLKKLQLKDGKVLKYKNKIIEKNREINRLMRILNEHSILDTSQTSFNGGGSVNSSTGNAPGRASPPPAKPKSHSSRNKEKASDMLRTLGMLASQVLNDEADDDSANQTIVQANNSGNRAVDCNTTEPEIFHSPNVSAGTQNSDSINQMRGTPEPRSSSMGANYFLKQLPIQMPKMKRFSTVDGTVKDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T591</accession>
    <name>A0A8J2T591_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Protein farnesyltransferase subunit beta</fullName>
            <shortName evidence="9">FTase-beta</shortName>
            <ecNumber evidence="2 9">2.5.1.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_11452g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins. The beta subunit is responsible for peptide-binding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-cysteinyl-[protein] + (2E,6E)-farnesyl diphosphate = S-(2E,6E)-farnesyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11535"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:86019"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.58" evidence="2 9"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T591"/>
    <dbReference type="OrthoDB" id="10261146at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005965">
        <property type="term" value="C:protein farnesyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004660">
        <property type="term" value="F:protein farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097354">
        <property type="term" value="P:prenylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02893">
        <property type="entry name" value="FTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026872">
        <property type="entry name" value="FTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF6">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="10">
        <location>
            <begin position="69"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF88489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="416" mass="46317" checksum="C19D4301E1E3A721" modified="2022-05-25" version="1">MARKIKDLKDTNLLGRKRLPIERVVSSEDGAYEEVVPMVKEIETETTEAREELLQKCQAIFTRTDFPVLNRDFHKKFLESPLSHKLPPGMVSLDASGPWMIYWVANGLKVLDESCLARETQRRIYQKLFGISTVGGPFGGGLGQLPHLASTYAAINALAICENVDNCWDSINKDSIYNWLLTLKRKDGGFQTCYKVGEYDTRGVYCAISVASMLGLLTEDLCKNVVQFLVDCQNYEGGFGAVSHEDEAHGGYTFCAVASLAILGALDKVNLDKLADWCSQRQFNGEKGLSGRSNKLVDVCYSFWVGGVASILEAYGYGPCIEKDALRDYILCCCQMTNRPGIRDKPGTNPDFYHTNYGLLGLAITENTFRLDKTVPHAFKITSTIIDQDRASGLTAINPVYGLPNKDLELFYKHFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5B0</accession>
    <name>A0A8J2T5B0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S03-02124g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_02124g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5B0"/>
    <dbReference type="OrthoDB" id="27832at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039762">
        <property type="entry name" value="Nmd2/UPF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007193">
        <property type="entry name" value="Upf2/Nmd2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 2 UP-FRAMESHIFT SUPPRESSOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839:SF7">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04050">
        <property type="entry name" value="Upf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="34"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="369"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="574"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="806"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="960"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="806"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="824"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="890"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="960"/>
            <end position="978"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF88796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1037" mass="120866" checksum="7C0BE42D930216CC" modified="2022-05-25" version="1">MDDEQRLKLSELNARAWTGEEVFCLKQAKLDSSIKRNTGFIKKLKLGITKDSKTLLLKDLGEVSLGKYLSEIINTADEGLQNVSNKSDDVAAAVEVISGLHQRFNAQFTCGLLELFLNHFAYPQDDSISDKEAIANVNKLKTNLKVFTELYLVGVFTSLDNVTSKEGLPAFLRKRTIRKEPLLFSILKEILTYKFNLGYSAAIASSFVKKYPQLFEDDNIADEYLYDKDLKNLLRTLFRKYTEAVFIKAVQLKKKSTKLIKEHQKCQIRTGKETDDFLDEYNSIVPQYEKFKSVALLLADFFKLEAPELNDEVDTKEGEEIKPSLITNAVPPPGQKVWENEEVRKFYEVLPNIDDVHDQSSENNSDSSNEKVNTFFKDLEEAESKEAIDTLSVAYWSQNLDNKATRKRLIKFFVESKDWSKVRIYARFFSTNSKYFPELIEEFVTYIDTGFRSQLHSSKINVKNIIFFSEMVKFMQLPVYMIFHKIRTLIINLQVPNNIEILTVVFEHLGIFLINKPEYKPHMEKMMGLLKEKKRDRQLNMNLKGAMDNLITLVYPPSIKSLNASTRELLPEQQFYHILIRRELQYFEAKRVIKLLRKAHWKDKEITDTLFDIFVKPEELSYQNIALLARVLSELYKYQRNFVIDCVDELFEKVERGLEVNDFSRSMHRVAEVRYLTEIYNFGLIKPDILLDVMYMIMKHGHDGKGSYLFCPNEIDNVDNYFKVQLIATMLLNLRRTTSTLKKKLPLFLRFFEYYTFTKEQPLPQETQFRLKTTFQKFSDQDGFETSESLQESATKLTSMVKTLSNVNSPTSEMTANNNANEVSSEDESDRDREQEDEYEDEFSAVEDTIEEAEEDEEFDDQSEEADGNESDEEEETEDENEENDEVEDDSYKDVMVDRDIEQKRMYDEYQAKMKDEKERKMEEDLEKQLRKIMQDSMDSRKDQKVVTAKIPYIDQGKTSISSKTAVSEPRTSANDNGNGPKKVAFTFLNKSGRKTQSRTLRLPSNVDFVSGVLEEEERLKHEREKIKNIVLQRTFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5F6</accession>
    <name>A0A8J2T5F6_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DASH complex subunit DUO1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Outer kinetochore protein DUO1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">BN860_13190g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DUO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5F6"/>
    <dbReference type="OrthoDB" id="4067138at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013960">
        <property type="entry name" value="DASH_Duo1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28216">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DUO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28216:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DUO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08651">
        <property type="entry name" value="DASH_Duo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="144"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CDF88564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="208" mass="23516" checksum="94695092CA4EE508" modified="2022-05-25" version="1">MDSSTVNKLIPQLFEEMRSNLGKDTVDVNQSSQNSIITTQSLLKELEVLDKIIPMVESVDNSLRMSVPQHLDRINEICKSTNTMLDSWINIQSQADYIYKLMSSPNYIEHASAQLSEPGITSEELLAAEIQKNDTLKKQLKEEQDKNALRNNAHSGVKQETPRHPTSTSRISKPTARFDRSRAGRTSTPKVTERLARPTASSTRKMFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5I5</accession>
    <name>A0A8J2T5I5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_14862g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5I5"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019740">
        <property type="term" value="P:nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000003">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF10">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="392"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="10">
        <location>
            <begin position="33"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="455"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="481"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF88636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="494" mass="53250" checksum="6BBB3F59DE316FE5" modified="2022-05-25" version="1">MSMNFTGTPTGTGTGGNSLTTDLNTQFELANMAWIGVAAAGVWIMVPGVGLLYSGMSRKKHALSLLWASMMCVSLVIFYWFFWGYSLAFSHTTRGNGFFGTMKFFGFKNVLGAPSSVSSLPDVLFAVYQGMFAAVTGALMLGGACERARLLPMMVFLFLWMTIVYSPLACWTWNAQGWLAVLGSLDYAGGGPVHISSGHGALIYALILGKRNDPVAKSGMPKYKPHSVTSVVLGTVFLWFGWQFFNPGSAGNATIRAWYSAMSTNLAAAVGGLTWMFVDYFRYGGKWTTVGLCSGIIAGLVGITPAAGFVPIWSSVIIGIVCGVGCNLATDLKNLLHIDDGMDVWALHGVGGCIGCVFTGIFAADYVNSTAGSYTAPIEGGWLNHHWKQVGYQLAAMCSTIAWTVTITAILLLAMDFVPFLRLRLRPEEEEMGTDEAEIGEFTYNEDTLFVPEPIRSKTSAQVPQHENIDDKIIQPEEAEGNSSTAGSNELQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5V7</accession>
    <name>A0A8J2T5V7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ZYBA0S04-06216g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_06216g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NFX1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5V7"/>
    <dbReference type="OrthoDB" id="6512771at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06008">
        <property type="entry name" value="NF-X1-zinc-finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd06006">
        <property type="entry name" value="R3H_unknown_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.50:FF:000006">
        <property type="entry name" value="NF-X1 finger transcription factor"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR NF-X1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01424">
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    </dbReference>
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    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="92"/>
            <end position="141"/>
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    <feature type="domain" description="R3H" evidence="13">
        <location>
            <begin position="751"/>
            <end position="814"/>
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        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004123"/>
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            <dbReference type="ARBA" id="ARBA00007269"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
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            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022771"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
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        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
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            <dbReference type="PROSITE" id="PS51061"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="CDF89520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="973" mass="109424" checksum="C798CDC09F5288BE" modified="2022-05-25" version="1">MRRKFTSVQKHVGAMSGGQELSQSVMAVGEYDCDAEPVLQAADKSLVLEFSDDSDDMTSESESEDEDEDEDDNMMFYERAVRDISSGDSYVCMICTVEMDYTCRMFACPQCYRVFDYDCIREWAIKSTSKTVEGIWKCPNCYFASKKVPRKDRPTCWCGKVVKPDPNPLDPNSCGQTCDAPICRHGCSKVCHLGPHPPCTRIIGIRCRCGKHMKDVTCCETRGSSRPQFSCDEKCGMLFPCGIHRCEQKCHSGLCGPCKETLVAKDGSATIRCYCGLQKKDTIKCNDVRVAEKDSMDESGEKWVGVFACPEIRTVQYSCGLHSFVESCRAGPAISKMLPCPFSPQLLKTCPCGRTLLNELGNNRKNCTDYVPTCESRCGKPLPCGKHTCPFTCHSGDCMDPCTQFDKISCQCESKHYIVPCQFKQKARCHTKCESLMSCRRHRCIERCCSGKPSAEKRMKTPFSSRELQDETLVEAEHICLKDCNLTLSCGIHKCRRKCHPGKCPPCLESDPNDLVCPCGKTVVTAPVRCGVRLPPCPYPCISVVRDGYPCGHKPMPHCCHPVEEPCPPCTASVFKPCKCGKKSKVRTLCFQQDVSCGQICNKPLTICHHTCQKKCHDDSCQTKCRQICKKRRMNCDHKCPKPCHGKANCPDLPCSYPVIIKCACGRKETNEPCGSNSERPSATFTRLLPCDEECERVKRCAELKKALGLTESESSKIKGPESSLALLATTFEELDLPFTESALSVYSSHQKYCDGIETILNDFIDNKGKNSLHFKPMRPAQRHFVHELAKAYKLYSESQDREPKRSVYVKKESDGQSGRPAIMLNEALPIYQAFKQREKENKLRRSEMQSVTKLVNYTPKHEPTVELAKFNGFLVRNLTKGTTEHDLERAYDEHLRCTLVKDPIYKMLPKGNTALIFPTDYANVTMNTERDMERLVGHFDFICKESFIGDGVELCQVDEHIFSEIPQADKMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5X4</accession>
    <name>A0A8J2T5X4_ZYGB2</name>
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        <submittedName>
            <fullName evidence="3">BN860_14620g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_14620g</name>
    </gene>
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        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CDF87793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
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        <property type="term" value="P:vacuole fusion, non-autophagic"/>
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    </dbReference>
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        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
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        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="IVY1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38407">
        <property type="entry name" value="PROTEIN IVY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38407:SF1">
        <property type="entry name" value="PROTEIN IVY1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="25"/>
            <end position="54"/>
        </location>
    </feature>
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        <location>
            <begin position="278"/>
            <end position="378"/>
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        <location>
            <begin position="201"/>
            <end position="228"/>
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        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
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            <begin position="316"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="336"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="378" mass="41745" checksum="E0402ED0639C63B0" modified="2022-05-25" version="1">MDKPDSGTVSPGRYAPHLSEFYSIVNNTNPSSRQGIDGGHGERRDSNSMAAPSVTSNFSSISDLRTLVTKRDIRNTVDAMISLLESSMAYSASLREVSRNASLMGHSLENFARLKGCSDDNAEKLLSAGGLFHLLANHENIMSQCLKSALAENLLDEIDEFKVKSKTMENEFRVHAREESLKLKLQEKHNIKLSKRKTRNLLSYKESLVNLQTQLDQLESLRHDYYQDSYNLVESTCTKVLDAVATVSRARVEISENIARKGWSGGGLEELLMDAEDPFNKEESPGDTNYASGEETIEDEHTKSPKMSLSSSEPRPSSPSPPSPTDPDSMSHDDGVNNSFSLPLAGSSKSQEEPINNDTELLSELAGMHMNTESHNET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T642</accession>
    <name>A0A8J2T642_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S03-09758g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_09758g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T642"/>
    <dbReference type="OrthoDB" id="196165at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053039">
        <property type="entry name" value="Polarity_Bud-Selection_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47775">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47775:SF1">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="192"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="337"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="535"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="153"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="337"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="372"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="402"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="420"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="439"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="474"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="495"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="579"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF89125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="637" mass="71238" checksum="2ED072CFA51D58B6" modified="2022-05-25" version="1">MSQGPSVNAFHGSISPSLLPAEFGGKLSLDDPDRRDLLNDPSLTENYADLVKMKPNLPTEHEGESNSGSVVVTGESPVKRSGGSSPGNYAGLPEAEEQRDEDYDYSDSEFEESLEKRLQDMDTGLPGDRRGFSGGGSASGTNSVYDGLMMSLSEEDDDDLHLEDEDEDQQEDYEGAFEDEEELQPLPPPQELDPDKLYALYAFSGPDPSHCQLKQDEPCTLLNDQDAYWWLVRRNGDNKIGFAPAEILETYPERLARLNCWKNENMSPRKAGWIAENGLEYEQDHEPGQSDDDEEEGKTLHGEDDMSNPRLALKEYGKGNKSVSFNDVISYADRYIEDEDDKNDEDEPNQYDEFSESTLKTSRSMEDDDLSEATSDASFNTASMVPLNVEKRRQTPEQQLSQKDKENTSDEEIVKEINENRTSFIETHDGTQQNGSFGRAEDEPIDEKREEKSIEETQDGLHKIFEAPVLPFSRKTSQMETSNSDYSISTIGEFSPSSSEWTSDSPQQHIADHQSPRTIPPSRAIQDLTKIVGKDDESAATLQVDQEQKSKSIKSRHIKETPASSSSSSEESFMEAERVASSTSVNSTSSAPRQQLFHSTSYISATSGKHHPLIDRLYNPIFDRMDDLLKEIDKAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T650</accession>
    <name>A0A8J2T650_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_17128g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_17128g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T650"/>
    <dbReference type="OrthoDB" id="1922282at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018961">
        <property type="entry name" value="DnaJ_homolog_subfam-C_membr-28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39394:SF1">
        <property type="entry name" value="DNAJ HOMOLOGUE SUBFAMILY C MEMBER 28 CONSERVED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39394">
        <property type="entry name" value="YALI0E31793P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09350">
        <property type="entry name" value="DJC28_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DnaJ homologue subfamily C member 28 conserved" evidence="2">
        <location>
            <begin position="175"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="382" mass="44220" checksum="E492D8BCD871000E" modified="2022-05-25" version="1">MKSITRIAVFKNSGLRFQSSNSNGGHLMRRLQDLKDQSTVDTNDPLVKITQLNESDFVNDWATEEDKLNADKVLEIIDAQKQAAKTNQGISSLKREENGSDTASQAQREAIQYINKMPSITFREKLETAQNRAIRYKLRQGKINQAKDDNEQLHFKELYGERFTPIGSFEKLESLADRRIEESMKQGGFKDLEKVRGKPTKLPKPNPYLSTTEHYLNNILVKQNIVPPWIENQSRVNQNVTELRSDLLRGFEGELATFLRKFKLVNASSDLKLVSSSIASSYGSVEEFIQYRFDNWKLKQKPTVDTRIDVINSALRTYNLQAPLPAQKLYLLPEKEFQRVRDEANLRRVVDEEVQNLKHRQYEAKQNVETTILSFRGLFKFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T679</accession>
    <name>A0A8J2T679_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN860_06832g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T679"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11651">
        <property type="entry name" value="YPK1_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000048">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="363"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="17">
        <location>
            <begin position="619"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="13"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CDF89001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="696" mass="78903" checksum="7FFA27AFFC367D31" modified="2022-05-25" version="1">MYYWKISKFKFGKSKDDKEGERDKDKDRTGHHGGHNGMFHHHRDNSRQSADIECSESGEMGYMRPSMDRKETIVPSSSSIAPVRLSHDASSSASTVREPSGNPEDAFADSRRSDDFAPPDMPITAPPQSTSAPGILTIKVYNGDGFSLPFPITSNEQILGKLLSSGVTSKRSSLSSEVDALVSQLSRMQLQKTGPADESLLPGDVCTRFIPSTIMLPGSEQLNPLLYFTIEFDNTVATIEPEYGTMVQPVFNKISTFDVTRKLPYFKIDVFARIPSILLPSKTWQQETGADDERLRVMFDKINSNQDIHLDSFLLPVNLKIDSAANIRLYNHHWVRLENGWGSINFSVDYKPSKNKSLSIDDFDLLKVIGKGSFGKVMQVRKKDTQKVYALKAVRKSYIVSKSEVTHTLAERTVLARVECPFIVPLKFSFQSQEKLYLVLAFINGGELFYHLQKEGRFDLSRARFYTAELLCALETLHSLDVIYRDLKPENILLDYQGHIALCDFGLCKLNMKDKDKTDTFCGTPEYLAPELLLGQGYSKVVDWWTLGVLLYEMLTGLPPYYDEDVPKMYKKILQEPLRFPDGFDRDAKDLLIGLLSRDPKRRLGYHGSEEIKTHPFFSQLSWKRLLMKGYIPPYKPPVSSAMDTSNFDQEFTREKPIDSVVDEYLSESVQKQFGGWTYVGNEQLGSSMVQGRSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T692</accession>
    <name>A0A8J2T692_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mediator complex subunit 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_03334g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 12 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20828at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019035">
        <property type="entry name" value="Mediator_Med12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46567">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46567:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09497">
        <property type="entry name" value="Med12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01281">
        <property type="entry name" value="Med12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med12" evidence="8">
        <location>
            <begin position="118"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1348" mass="157627" checksum="76603B426C9EA9D9" modified="2022-05-25" version="1">MTPNKYILTPPDDLHPYVPKRENHQKEVYPDFDPWRHTKEEDEILLNYVSKGYYSTSKVNFESISARSSLQESLPKLSDQLADQFSQVLHIREGQVNKIFSGRSDSNGPFFNDLCGPGFALPSRITLTEHRRELWLQELSSPYASLHKLSKHIPHGLKRRQVLEQCYIKRIPLKRAIWLIKCCNSIEWKALIAKQQQKREDKIDVNALLLKEWTDNFVYIMEKLIFEMTQHYNDPAQLKRWRGEVGYFLKLLGNCYKMGLLDRDIFHHWLIEFGAKVENFEFLPLTLHIIMIFWDGICQKAQGSSLSQPSFLVSKTAEMLLNKYYTISHSKSMIDDDKYIINDIKKNNKIKESILSTLQMLICKLFQDQSLEAFIFPNSSWDLYKPILYEVTNKLQNTTDEGLKCRKKLELISYRNESLRFNSSLREREDESDTGSKSIGSGTGIKSDLDICRLATVDTKFTQMLDDNPLGFDWSSYVDRSMHHVEQIRQLCLWAIHPSKKSHYEASQLAAKLLLLKINSTDGFQEYVIEDVIWSLIFQVAKLTEHERCFLVDLELLYSLLNVLITYGILKVSTYVRKLISSGILYLPESNDKFMHCELLINLKISPLMKSQYNMVLRNVMEYDTSYYQKYNFDQLLRQSEEMKQNINQGDDLKIKSYPLSMKIMTAEWYLTKLCSEKLLPVNRSILMKNYQIFCVDLKAFHHYYKWIEFVVYHQLLSDIESLEALMDILLCYGKLFSQLINDHILFTKTFIFIYTRILREKDAGSYAVTSFMPFWKFFVKSFPYALNADEELRSELSAIYEEEKAKSERLGKNRQEALQVYNLLKELGPKSASLNFAEVFSTSLRSFLSLKGSDNEQKKFRNNLLLLMSSNLRDYNKFMSIYLKRKDFELTTLKYLISYKLLTFDIVHNILGTPFILNLLNERDPPQSIYFELYKDQYVKNNYSGLLAGCLNNSSQSYELLIGLLVRYGTLSSLSSEASKTIVKLFRESQMQSFEILNDILNYGNTKKDTVCDISIENPAYSYRMLNFTNLWAFQAFTNYLIEGIISKNNTSNSYLHEFLFEMVNNTNYNCLCAHLFDRIEETPIIEQVAQVFEEDFFHRCFSKNHVNRNYMAVVVEVLTSLSQKKHKDSSNDLNISEGNFELLHQTMNLFCKMSETEIQGMEMQLDIFLKIFSIHQNVLFHYIVTSIQNEGPQKTNGLINVMYALFDKISFNLRLKLMLYEILSSLKSYCIFVSTTGSDNGSRFEVPEQLLKLPPFQISSFMKDEDDSASAEEKIELGISTVNIKIKDNTDKWFIFNKREDQYWCKLHNEPYHFINNFQSGAASSFNNSCLNLSLFNAIFRRQNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6B8</accession>
    <name>A0A8J2T6B8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Valine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="4">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Valyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">BN860_03774g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tRNA(Val) + L-valine + ATP = L-valyl-tRNA(Val) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="4"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6B8"/>
    <dbReference type="OrthoDB" id="629407at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00817">
        <property type="entry name" value="ValRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.380:FF:000001">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000009">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000020">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000078">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.740.10:FF:000005">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.380">
        <property type="entry name" value="Valyl-tRNA synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02004">
        <property type="entry name" value="Val_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037118">
        <property type="entry name" value="Val-tRNA_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019499">
        <property type="entry name" value="Val-tRNA_synth_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004349">
        <property type="entry name" value="PRK05729.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946:SF109">
        <property type="entry name" value="VALINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="VALYL-TRNA SYNTHETASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10458">
        <property type="entry name" value="Val_tRNA-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 14" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="17">
        <location>
            <begin position="161"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="18">
        <location>
            <begin position="829"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="domain" description="Valyl-tRNA synthetase tRNA-binding arm" evidence="19">
        <location>
            <begin position="1039"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="55"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="1044"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF10458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CDF88868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1108" mass="126227" checksum="62580D67B9D78A58" modified="2022-05-25" version="1">MHKCLRLVSVVSPVKYRAYFSTKTDTSLWSSKKFLNKRPSVVSQQVRRFTMSDLEHLPPVDPKTGEVIINPKKEDGSDKTPKEIEKEKKKAEKLLKFAAKKAKQQVKKASSDNGKKKNKKKEVEPVPVFVDKTVPGEKKVLLPLDDASLKAYNPANVESSWYDWWVKSGFFEPEFTADGKVKPEGMFCIPAPPPNVTGALHIGHALTISIQDSLIRYNRMKGKTVLFLPGFDHAGIATQSVVEKQIWNKEKKTRHDYGREAFVGKVWEWKEEYHERIKSQIKKLGASYDWSREAFTLDPKLSKAVVEAFVRLHDEGIIYRDNRLVNWSVKLNTAISNLEVENKDIKGKTLLSVPNYDEKIEFGVLTSLAYPVVDSDEKLIIATTRPETLFGDTAIAVHPDDPRYKHLHGKFAQHPFLDRKIPIICDSEAVDMEFGTGAVKITPAHDQNDYNTGKRHNLEFINIFTDDGFLNENCGPEWQGMKRFDARKRIIEELKGKGLFVGQEDNEMTIPTCSRSGDVIEPLLKPQWWVAQSAMAKEAIKVVKEGEITITPKSSEAEYFHWLENIQDWCISRQLWWGHRCPVYFIEIEGQENSRNDDKYWVAGRTLEEAQEKATAKFPNTKFTLHQDEDVLDTWFSSGLWPFSTLGWPDKTKDMSTFYPFSMLETGWDILFFWVSRMILLGLKLTGTIPFKEVFCHSLVRDAQGRKMSKSLGNVVDPLDVIKGIKLDDLHAKLLTGNLDAREVEKAKAGQKESYPNGIPQCGTDAMRFALCAYTTGGRDINLDILRVEGYRKFCNKIYQATKFALMRLGEMYHPPAKEGLSGDESLVEKWILHKLSIAAKDMNEALDKREFLPATSQIYELWYSICDVYIENSKYLIQEGTEKEQKSAKDTLYTLLDNALRLIHPFMPFISEEMWQRLPRRADDHVPTLVKAAYPTYTAEYDDAKAAKDYDLVLDITKEARSLLAEYSILKNGKVYVESNHSESFETAVSQRDSMVSMIKAIDEITVVRKASDIPEGCVVATVNPEVSVHLLVKGHIDIDAEINKVQKKLEKATKTKHSIESSISSKDYETKANAEVKEANKVRLENANAEIDGFEVTIENLKKLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6F9</accession>
    <name>A0A8J2T6F9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S03-11144g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_11144g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6F9"/>
    <dbReference type="OrthoDB" id="18781at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17923">
        <property type="entry name" value="DEXHc_Hrq1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18797">
        <property type="entry name" value="SF2_C_Hrq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001137">
        <property type="entry name" value="DEAD/DEAH box helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055227">
        <property type="entry name" value="HRQ1_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018973">
        <property type="entry name" value="MZB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957:SF3">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09369">
        <property type="entry name" value="MZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22982">
        <property type="entry name" value="WHD_HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="292"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="514"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF89184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1070" mass="123150" checksum="739E711E4372F89C" modified="2022-05-25" version="1">MSPKAAKRVKPNDVSQIDRLKKIFFRLNTLYTFLHCKKHVIPTFKTLQTPIENALKFPITELDFAKIAALMPRDCVFKYIDENQIYTETKEFDFNNGGWQQKDNDIYQLKDVNEEFQKARSTQILIFEFVDGNMRYSWQQTDKGRVQLPTYSADEMKKMINKRKHHFENLLDAFLSKQQAEGVDPDTELEMLAKRFVPKEKDYEDPIEAMMKSKQKKESMVILDEANGMERPTIENMVVELQQSENYNNQITSHYVIPEKTAQYKDLDFKLAEEIYNALDIKNFYTHQADAISAIHRGDNVIITTSTSSGKSLVYQLSALDLLLKEPNSTFMYIFPTKALAQDQKRAFEELLSKIPRLSNVIVDTYDGDTDPNQRLFIRKNAQVIFTNPDMLHTSILPNHPNWRSFLYNLKLVVVDELHTYKGLFGSHVALVLRRLLRLCQHYYENDALRCISCSATLKRPIQHMKNMFGINETVLIHEDGSPRGAKHLVLWNPPVLSQHVRRREDFIHESAKILVQLMLKNVRTIAFCFVRRVCELLMKDVRRIFEEMEMPEMIHEVMSYRGGYAASDRRKIEREMFHGNLRAVISTNALELGIDIGGLDSVLMCGFPLSMANFHQQSGRAGRRNKDSMTLVVASDSPVDQHYLAHSEQLLNGDNSDCFQELVLDFDNVLILEGHIQCAAFELPINIDRDKTYFKEDKLIKMCQERLDYTSDGYHTNNRFLPWPSKLVSLRGGEEDQFAVVDITNGRNVVIEEIEASRTSFTLYDGAIFIHQGYPYLVKEFNPDERYATVQRVDVDWVTSQRDYTDVDPQEIEMIRSLESNDTPVYFGKIRTTIVVFGFFKMDKYKRIIDAIETHNPPVVIDSKGFWMDIPKRAIDICQAKHLNLAGGIHAAQHALMGLLPRFIVAGVDEIQTECKAPEKEFAERQTKRKRPARLVFYDAKGGKYGSGLSVKAFEHVDDILNGALERIEECPCEYGCPNCVVATFCKENNFVCSKPGAQVIINCILNREDAVFLDNIKEGPEPNMPEIKVETVVPVSDHVNFSPDFQIIDVRKKKEEIITGSLAIVKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6G4</accession>
    <name>A0A8J2T6G4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ZYBA0S03-05226g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_05226g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6G4"/>
    <dbReference type="OrthoDB" id="10027144at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057778">
        <property type="entry name" value="KH_Vigilin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24668">
        <property type="entry name" value="KH_Vigilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="136"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="583"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="657"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="727"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="806"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="884"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="1081"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF88931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1163" mass="129227" checksum="86ED4C9118471665" modified="2022-05-25" version="1">MSKLEETPPTSAESQLPSLKDLPSLGSNAGFSNRKVAWGPNVKPTVVPATPQPVPTTGGGSKPVRSKTIQEAFTLDLQSQLSITKPEFSRIVQSVKHDHDVSVESTLSKTSRTFLISGLPANVSAGRRELVKKLTKPVTDVMQVPSRAKAAIIGSGGRNIREIQDSFDVKINVARENNPDAYDEDLGDHLADVSIHGDVDSVKLAKQKIAEIVREDTKNSSITIPVENPHLLPFVDVSTLQLESVKAQVNDGDIVISGPLEEIKLAKAKLQSYLKQLESQLTEEQVKIPVKFQFLIDNDEIKEKFNVVVSSASSDDLVTFLGHRDNVKEAIAHARSSSIAYAVDTLDISKAHSKNLQHAKNLVLYFEKYNVLQNIKNAHEDIKIVLPEPQQLPKVDSVCVYISGKSDKTEEIKSVRKELIALVNDITPFDTLSVGDLDYELFHKEIKHILLASEESTAFVQFGDYYEGDDTILLVARVSNEDFKPSLEEIKESLARVNSTLDPLRNKQNNLSLEIVDLESPQQDSLLNEVTLQLLLEEANQDGNTIQIKMHTPSANQITLRGNEKAVKITKKAVDSIVSSPTNKFKDTLDVPANAISRLIGSKGSHTQQLRQKFDVQIDVPSESSNGKTVEVTITGVQYNVERAKSFIQQEAKKWADIIIKEMYVAQKYHRNLIGPQGAYRNRLQDKYNVRIFFPKNEDTVTIRGPSRGVTKAHEELKSLLDFEMENGHKVTMEVPSEHVSRIIGKNGEMINDIRADFGVEMDFLQKTTDPEVQKTGQVQLEITGSRQAIKDAQAKVKAIITEASDFTKTTLDVDRKYHKTIVGAGGHTLKDIITRAGGDGLRNRMVDVPNANSEVQVIKVEGPKKFVDAVVKEINKIVEEGENSVSKELDIPQEKQGALIGPGGFVRRQLETDFHVTLHVPNKGESGKVVLSGLPANVEKAEKKIFSEIIRDNYDHELMVPAVLHEFVAERGAFMQSLRFDYSVNVKHGNTGRKANKLVRRPVDIPVDKVRGESEEKFKVTLEEVEKPEVDETSEKIAWRLIYEDFDLDELLSEDVEENKKSDKKTDEAKKQESLAKAIDAIEKRISLAKDATTVGYVWSSEPSKFNKIVGPGGSNIKKIRDATGVIINVPRKSDKVNDVVYIKGTKQAVQDAVQQVLKTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6J9</accession>
    <name>A0A8J2T6J9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein transport protein SEC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_12288g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6J9"/>
    <dbReference type="OrthoDB" id="2228at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="665"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="678"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="704"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="721" mass="82897" checksum="CC3DB10BC64DDA50" modified="2022-05-25" version="1">MSDLIELQRANLLSLLSEIETPHNVKFLVIDSEVERFLSFLFVNPGDLLRHVTAVDRIDSPHRKGQPSVEVIYLLKPNKYNINCIDVDFRLRPPKYRRSHVRFLPGTGPEMVDYFKSKRYIPQYLATIKEVELSFIPKETQFFLTMDIDRPLQLFFNKQCINLIDRTIKRTIHSLLNLCVVTGEYPIIRYSQPQTNEEKLTPATLLAKRIAFEFQTTLDNYAREHQEYPPPSQRPRAVCLITDRCLDLFSPVLHDFTYRSMVYDLVSDVDPSTDIYHYTAENEKGEQEEKSSKLLDLLDPDWQELKNQHIVDANEYLSGRIKEMIAKNPLLVDRSNVKTTTDLLSVVAHLKDFDEERRRIILHKTLIETCLQANQERKLAESADVEQNLAGFGLDIDGEKVKNITYNLLEILSMKESNVIDKIRQIIIYALYRGGIIEQDFVKLLAFIGIESNHDFFSHFMLLFKNFDQVGFQLIKQEPKAKPYKKQWFHDPIVKDPSVYNTSRFIPAVGNILSKIIANPLLLSEEFFPYVKDKPIELLEEEEGSGYTAAANGSASLRNPRHKAAWTKNNTNPKRAPRQRFFYYIIGGVTQSEIKAVYEQAELKNRDVFLGSDGIITPLSFMKSIENLTSERKNLQLKDDEAEKEKPPDFMLDVFTPVAPPVSHVQYLPKKQPPHSAPASTQSGKPGQSASSESAGDEKAPGKEKKKRSKFSKLLKPNKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T714</accession>
    <name>A0A8J2T714_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ZYBA0S03-11254g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_11254g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T714"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07098">
        <property type="entry name" value="ALDH_F15-22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000024">
        <property type="entry name" value="Betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="5">
        <location>
            <begin position="113"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF89189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="647" mass="71841" checksum="2450C3B6758C0F6C" modified="2022-05-25" version="1">MSDVYLNADMLQQLNNTVQEYLTDWMKKQHYMFYNQSQIVVDQIQSNPKMAATTTFIACIFSIYVFFKFLWGGSSAESCKPCKFVVPVPDAAQTHWKGKRLFSTDIRDPENCNIIQAYCPATGQFLGKFPSKTAKEADEMIHEASLAQKEWAISSIDRRLRVLLSLKSFIMDNQDLIANVACRDSGKTKLDASMGEILVTLEKLQWTIQNGERTLQPSKRPGPTNFFMKFYKGAEVRYEPLGVVSAIVSWNYPFHNLLGPIIASLFTGNAIVVKCSEQVVWSSEFFVGLVRKCLEACGEDPDLVQLFYCLPPSGNDDTANYFTSHPGLQHITFIGSQPVAHHVLRSAAKSLTPVVVELGGKDAFIVLESAKNLEALSSIIMRGTFQSSGQNCIGIERVIVAGSNYERLVRILHDRMANHPLRLGSDIDHLEEVDMGAMISDNRFDQLESLVQDAVSRGARLLYGGSRYSHPNYPQGHYFQPTLLVDVTPDMKIAQQEVFGPILVVMKAIDTQQAIELANSATFGLGGSVFGSDFKECNYVARELKTGNVAINDFATYYVCQLPFGGVHGSGFGKFGGEEGLLGLCNAKSVCYDTLPFISTQIPKPLDYPIRSSQKAWGFVKSFITGSYTTSPWQRIKALYSLAKNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T734</accession>
    <name>A0A8J2T734_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Peroxisomal membrane protein PEX14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">Peroxin-14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_05710g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor. The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm. Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9 10">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peroxin-14 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T734"/>
    <dbReference type="OrthoDB" id="5549158at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025655">
        <property type="entry name" value="PEX14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006785">
        <property type="entry name" value="Pex14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058:SF0">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04695">
        <property type="entry name" value="Pex14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="3 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peroxisome membrane anchor protein Pex14p N-terminal" evidence="13">
        <location>
            <begin position="8"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="239"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="281"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="130"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="321"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF89498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="357" mass="40307" checksum="5BE87C987476CD0C" modified="2022-05-25" version="1">MSAKVQDDRKQLFESGISFLGDPSVKDAPLTKKIEFLQSKGLTEQEVALALKQSQHGQDEVSKTPESSSGVREISKDPIFYETVPPPLPRRDWKDYFIMATATAGLCYGVYEVAKRYVIPNLLPESQSKLDQDKKEVQEQFDKVDKLLETIEQEQSDFRIKEEQKLLELDKTVMDLHNCLEQTTHTREKIEDEFKMMKLEMANLQNKIDNFILSKKNDQQLDKISTEFESLKHLIKNRESSATGGFENSDRKSPHAKSPVPGVNAIPSAAEILSKMNFNKNKISNGNGTDNMANDSASTSIKDQEQPVPAWKKAREESLGSAASTNSIPEWQKSAMNEPTLQDWQNKPANTPQLSQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T764</accession>
    <name>A0A8J2T764_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="20">ZYBA0S04-00254g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">BN860_00254g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S8 family. Furin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T764"/>
    <dbReference type="OrthoDB" id="300641at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007323">
        <property type="term" value="P:peptide pheromone maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000026">
        <property type="entry name" value="proprotein convertase subtilisin/kexin type 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000005">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF14">
        <property type="entry name" value="NEUROENDOCRINE CONVERTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 15" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 18" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035269483" evidence="18">
        <location>
            <begin position="23"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="678"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="19">
        <location>
            <begin position="455"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="605"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="760"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="611"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="631"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="760"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="779"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="14 15">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="14 15">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="14 15">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CDF89268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="799" mass="88525" checksum="67E752B52FDE7ACB" modified="2022-05-25" version="1">MRASTIITILFCGWAVVGTAQGVPQKDHASRRYFAVETELMPDDVMNIHPLWRFEHPVRGLDGHLVFSMEKEQYQIYKRGDRVDEDSRILSHEELLPHRLHKKVPVEQKPPMDSSMVPLKDAESRLDIHDPLFEKQWHLINTNYPGNDINVTGLWYENITGHGVVTAIVDDGLDYESDDLKDNFCKEGSWDFNTNTQLPKPRLEDDYHGTRCAGEIAAVRNSVCGVGVAYDSKVSGIRILSGEITAEEEASSLVYGLDVNDIYSCSWGPADDGKHLQAPTDLVKKALVRGILEGRNKKGAVYVFASGNGGALDDNCNYDGYTNSIFSITVGALDHKGLHPSYSESCSALMVTTYSSGSGEFIHSTDIKGQCTDQHGGTSASAPLAAGIYALILEANPNLTWRDVQYLSVLSTKELNDYDGDWQMGALGKRYSHKYGYGGIDSYQMALMAKEWKNVNPQSWFYAPTTKVSEFTNETSKELESVLKVSKEDLKKANLKRIEHVVVTVDVDADLRGATTIDLVSPSGMVSHLGVVRRFDDSTEGFKDWSFMSVAHWGEQGVGDWKIRVNTKQDGQGVHFNTWRIKLFGEASDASKAVPFKFGNDREAVTDDDQAASASSVSTTSQPHASSTHISTTVSKTLTASASASSSTSKTSSTTIPTSTDSAYEDGSPKRIFPPTQAMHYFIALFAAGAVFLVLYFTVFMRSRRRIRRSRAETYEFDIIDTDSDYDSTMDNSMATNGRMLDAADVEDFDFDVSDEDYVVATDDQEDEEELPSAAEVNSQEEQEDQDSEPQEALHQHKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7A3</accession>
    <name>A0A8J2T7A3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ZYBA0S05-07162g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_07162g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7A3"/>
    <dbReference type="OrthoDB" id="283111at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14134">
        <property type="entry name" value="PKc_CLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051175">
        <property type="entry name" value="CLK_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="260"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="169"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="216"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF90025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="688" mass="77647" checksum="A5BDB833C6AFAB17" modified="2022-05-25" version="1">MRAFMSSHIGKKRSRNPTNMESLMQGMLAKQDSFLNNNLTGGGIYEQPFIDNENLQPLEDNDNDMSNAVEDDDDDVIFIKEQPVYLASPAVPASDNYTSTTSKKFKKQRTISLPQLPHAKLLYQGRREARGQNNDELLHLTGSSSRTLDGNRALSLRVVYSASPDDISERIYSSVSPPGSSTASASSDSVSSKEGMATATGASLNGSNPHKRSLRRLSAPRLAARLVGKKPRTKKRENFKTDKDGHYIYRQDDVFGGGRFLVKGLLGQGTFGKVLKCSDSGDFATSNPFKLNSNMVAVKVIRAIDRYREAAKTELRVLQAIRENDITGQYQCLMLQECFEYRHHVCIVTDLLGKSVYDFMCSNGVARFPGSHVQAMAKQLIRSVCFLHDLGIIHTDLKPENVVLCDESYVEKDLPPSVIRGLSTRRREASGGRRKLLKNPEIKVIDFGSAVFHKEYHPPVISTRHYRAPEIVLGLGWSFPCDVWSIACVLVELVTGESLYPTHENLEHMAMMQRVNGEPFPPKIVGKMFYKSSHNLGNLPSDLNSTVVKHFDKKTLSLQWPEKNKRGDYLTKDKSIRRVMDNCDRLDFLITSKVKADYGDWLSIDWNISPEKNWALIKSRILSERKTSGTTAGDLNKETFLFWYWFIDLCRKMFEFDPTKRITAREALDHKWFNLGILDEGITSFGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7B6</accession>
    <name>A0A8J2T7B6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ZYBA0S04-05182g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_05182g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18596">
        <property type="entry name" value="ABC_6TM_VMR1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18604">
        <property type="entry name" value="ABC_6TM_VMR1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03369">
        <property type="entry name" value="ABCC_NFT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000054">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000825">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF353">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN_PERMEASE VMR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="388"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="488"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="516"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="595"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1004"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1091"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1171"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1195"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1281"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1306"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="353"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="690"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="1008"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1370"/>
            <end position="1620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="442"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF89475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1643" mass="186035" checksum="15D9E99A7B7D4CD5" modified="2022-05-25" version="1">MASQHSSSCPFWVFDDVTQCGRVRYINYYFPLVLLVTGGVFLTYNILLHYYKYDKLRLKLRDITDLISGDDEDENKPLLQGSTISPYTGSEEVDGDELKERHFSLQRLRLVKPNGEPHGKSEIIRRGFEEKSRTIIEFLLLLAQFGLHAYLMVDYTDDETEFTRASSVVELVLWGFLLFIVSLRLWNVNQSVHWINKYCGNIWAISFTSYMFLFFSTVLPFRSIYIGHIKDATVRNYYLSQFYINLSLFALLFLSPSGNKWAIVYRTDDQITPSPEPVSSIASFITWSWLDKFVWKAHKSIIEKKDVWGLMKDDYAIFVLKKFKQYVAMNGEGRSFTRNLLSFFTKYLLLQGFWALMNSIMSFVPTILLKRILEYVDDRSSAPSNLAWFYVFCMLFCRIFCAIFSGQALFFGRRVCIRMKAIIISEIYTKALRRQVAVNSNKQSTDEVDPQKLNQEEHKDADEESGTSANLGAIINLMAVDAFKVSEICAYLHAFVEAVAMAFVAILLLYRLLGLAAIVGALLIVAMIPVNFKLVTWLGQLQKKSLSYTDKRIQKLNEAFQAIRIIKFFSWEDNFEKGIEAIREVELKLLLQRSLLWAISSFVWYITPTIVTSSAFAFYIYVQKQTLTTPVAFTALSLFSLLRTPLDQLSDMLSFVMQSKVSLERVQEFLEEPDTEKYNQLTVAKEGKKLAFENATITWDADKQDFKLRNLNIDFKIGKLNVVIGPTGSGKTSLLMALLGEMKLLSGRIIVPTLDARSMLRIEADGLTNSIAYCSQGAWLLNDTVRNNILFNSPFNETRYKDVIDACCLKRDFEILSAGDMTEIGERGITLSGGQKQRISLARALYSNSRHLLLDDCLSAVDSHTASWIYDNCITGPLMEGRTCVLISHNIALTLRNAELVVLLENGQVKDQGDPIALLHKGVLGEDELVKSSILSRHGSAVNLKSKSSSSIPNVAKVNEEDSKAAKTDEERIKAGKLIKEETKAEGVVGLEVYKWYLKIFGGWKMLAVLICAFVLAQGMYIGQSWWVRNWASHNIKDSLVNFGQNIASLGYNSFGRIVDRVGSFANTIGSVPNRIAILSGSDPVGPHSTVYYLMIYFLIGVAQSLLSAFKTILSFVAGIQASRKIFNLLLNKVLHAKLRFFDATPIGRIMNRFSKDIEAVDQELTPFFEGTFYSLVTCLSTVVLITFITPQFLVVAIVVTVLYYFVGYFYLSGSRELKRLDSISRSPIYQHFSETLVGVTTIRAFGDESRFMQENLHKVDENNKPFFYLWVANRWLAMRIDLIGSLVVFGSGVFILLNIDNIDSGMAGISLTYAITFTEGALWLVRLYSNVEMNMNSVERLKEYMLVEQEPYNHSVVTPPADWPQQGRIEVSDLSLRYAPGLPRVIKNVSFTVDPKSKVGVVGRTGAGKSTIITALFRFLDPETGHIKIDNVDITSVDLQRLRRSITIIPQDPTLFTGTIKSNLDPYDEYVDEEIFAALKRVNLVSQEELDRSHDDVSSENSENVNKFLDLNNEITEGGGNLSQGQRQLVCLARSLLRCPKIILLDEATASIDYQSDAKIQETIRKEFSGNTILTIAHRLRSVIDYDKILVMDQGEVKEFDHPYSLLLNKNSQFYSMCESSGELEILIELAKEAFVDKLNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7G5</accession>
    <name>A0A8J2T7G5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ZYBA0S06-06414g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_06414g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7G5"/>
    <dbReference type="OrthoDB" id="416217at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF49">
        <property type="entry name" value="LYSINE BIOSYNTHESIS REGULATORY PROTEIN LYS14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="20"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF90347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="641" mass="73077" checksum="0629AAA68B9AF944" modified="2022-05-25" version="1">MAEGTSPSVTRKRRKYSKLGCNECKKRKVKCDERKPECWQCTHLGKRCVYGQPSSQASFKSVKFVNSSNRNLRGQYRSSQEEHERSDVSVSFPAMEETSLSLADQFGEPNINSVLSDATILANDLVTSMVDPPITDFTLASEQIPFLLSPLNSSTNWDAISKDLSMISELELYYLKVFYHKVSFWVLPLASSPSENICNELLFDLIIKVNNQEGYQSSCLQSAVVSISAKYLYNTTKLKEHDVIRCTFLKKTIKQLTSEFDSMPQDYLLELKIESLILCVLLLTLDSSSFKTNEMRIHLNGASALLRKYQGLKEHGRGQFGDEMRNKCLLLSKAWFTATETVAFFSLVGTVFEDAVIDDMFKLGLLSPHENALKKMGLLTPNGYNIFLGYSTEVMNEHKAIMKCFSKPNLDDPYNNILFSLMVLTEAARSYKFVENEFGRIKIDEQLLASYPKQCYMTHKGEHYSVFDSLQQSAAECSFVFFAMTYLKWPPNCKIIQNSARRIWDFISWIFQDDYIVITDIHKFMVEVESGILVTYDQFRSKNLNLASKLIIPDMLHDFRGMMFQLNILTCGSLFLVDDTTTLRVTRAKFVAFCEYLVENIGAESGKSSLELLFKRWHLIKAGKTLSAEECLKNDAALPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7W1</accession>
    <name>A0A8J2T7W1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S04-08702g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_08702g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7W1"/>
    <dbReference type="OrthoDB" id="343092at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061982">
        <property type="term" value="P:meiosis I cell cycle process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20085">
        <property type="entry name" value="XPF_nuclease_Mms4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047521">
        <property type="entry name" value="XPF_nuclease_EME1_ascomycetes"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="2">
        <location>
            <begin position="355"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="648" mass="74162" checksum="0F8CB15CB1A798BF" modified="2022-05-25" version="1">MFDVIEISEESKEPESETVNHPEFYNLEDSSTVNGYNQTNYPSSPQRASQKKEAHIEIIESNPASSEVSVPFCHDLSSSRDNETLTFSASNQVEKSAGKAKGEKLLDKIVDVFSSPNSSLTSEKMREASTVPLKKLAANGPMSQNSTGTRFFGASDDNLKMQTSETVPSKASQDILANYPLSSPVVDPLSNSEEAEGDAIMQSSDHFFSNAKNQEPLPTKQQFPFLYTGIEKGTIELKLQKVHKPKERAIRSGSRRPDVVNLNISKFMKDKSDERTHSSSAGNIKPCKKIKIAPTHTISSNQYLENVKLQQYISRSKHYSHQESDALLRYWQEKDKKTFKRANQIHRSNENARSSIIIEMSSKLLSSFQENKSEIETFISPATLQTGYDNELPKIRFLRRSTSIYDLNHDIYYPSELKLLEENINLLFYDAQTFFELYRFQKKFLYDAIQSFSKNNKYLIVVLSDVNKLKKSMESLEDRKYKQKVQEQLTGSQQASSHNKKVEAIKTLQMSAFDLEQRLRHIDRLWNVKLHIVSSTMEFIATLPNLVSIVGRQRMDPAIRFMRYSHLHVRSSKDKTDTLRQTLHQINKMPELKCNRVINAYPTFQSLFADFNKGQLKAGLDGKHLMTEAMESRLYKLFTCENPNETIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7X0</accession>
    <name>A0A8J2T7X0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U3 small nucleolar RNA-associated protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_03620g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
            <location evidence="1 5">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the NRAP family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7X0"/>
    <dbReference type="OrthoDB" id="10251401at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000022">
        <property type="entry name" value="U3 small nucleolar RNA-associated protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3030:FF:000002">
        <property type="entry name" value="U3 small nucleolar RNA-associated protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005554">
        <property type="entry name" value="NOL6/Upt22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035082">
        <property type="entry name" value="Nrap_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035367">
        <property type="entry name" value="Nrap_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035368">
        <property type="entry name" value="Nrap_D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035369">
        <property type="entry name" value="Nrap_D4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035370">
        <property type="entry name" value="Nrap_D5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035371">
        <property type="entry name" value="Nrap_D6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972">
        <property type="entry name" value="NUCLEOLAR RNA-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03813">
        <property type="entry name" value="Nrap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17403">
        <property type="entry name" value="Nrap_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17404">
        <property type="entry name" value="Nrap_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17405">
        <property type="entry name" value="Nrap_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17406">
        <property type="entry name" value="Nrap_D5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17407">
        <property type="entry name" value="Nrap_D6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Nrap protein" evidence="7">
        <location>
            <begin position="205"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="8">
        <location>
            <begin position="359"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="9">
        <location>
            <begin position="517"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="10">
        <location>
            <begin position="700"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="11">
        <location>
            <begin position="903"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="12">
        <location>
            <begin position="1077"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF88861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1221" mass="139295" checksum="365E7339D345E502" modified="2022-05-25" version="1">MSGVKRRVSEVSGSTTNEPNNKRALSETGEDVPSGDTLSLKHAKLKENSNEREEDVEEEDVEKEDGEDEDTEVNRATSKGSSHNTAAHDIHIVRETAELFKSNIFKLQIDELLQQVRLKDSHILKVEKFLHMLHDLIHEIPAWEERSLAEADSFFKGKIVSIPFVDPKPDLSTTNYKVNYKTPAISLVGSFALKAGIYQPQGSSIDVLLTMPEELFEKKDFLNFRCFHKRSVYLAYLTYHLSILFEKNKMDTFLQLQYSYFNNDRLLPILKLSCKSQGTNVSEFNFHKTKFSINLIIGFPYRVFDAKKLLPNRNCIRVASGEANQALPATPLYNFSVLSSTTYETYLKYLYKTKKQTESFIEASILGRLWLQQRGFSSEVAHSGSLGGFGTFEFTVLMAALLNGGGNNGNKILLHGFSSYQLFKGVIKYLATMDLCEGGHLQFHSDNHSSSKYVEEGFQIPTLYDKTTKVNVLSKISVSVYEILKMHARQTLTMLNDVVRDQFANVFLTNISKMDNIRYDICYDVMLPNGSRTRPEDLTNFSFGPLERIKFISLENFLANKITNAMKYALGDRIKFIELELVGQKSSFAVSKRKVTSGTSSHLNFDHIRIKLLVNPAEAEKLVTKGPKHFEEPTPDATHFKNFWGPKSSLRRFKDGSISHCCVWSTSPSEPVISKIVNFALRRHLSEGCHILNDRTKHLQDLLPLPNLPASSKTSVLNLNSFYNLKKSFDDLYRVIFEMNLPLSVKSILPVGQAFRYTSLCQPVPFAFSSPDFFQEVVLEFETSSKWPDEITSLEKAKTAFLLKIQERIQSVQSDRYKTFFTRDESIPYNLDIVLLNILTPEGYGFRFRILTERDEILYLRAISNAKKELVPVLEKTYMKFTSKYVASVRHTRTVETLAHSLQLYSPVVRLFKKWLDAHLLLDHLSEELMELIALKPFVEYAPYSIPGSVENGFLKILKFLSQWNWKEDPLILDLVKPEEDTELSSENIANFADRMNLSQFKAIQANFHNLRKTDPNGMHVQFFVASKVDPSGILYSSGIPLPIATRLTALSKVAVNLLQAHGLNKQTIDLLFTPALKDYDFVVYLKTPIPLKTSSGILDSAEFKNLTTDQLSNVFPSDTSQLSRRMDPTYQLVKYLNMKYKNSVIFFSHRYIAVNGGEKGDRNVITGLIKPLYKRPHKFRVNMDCNVKPIDEQSVELNRDSIFHEIAAFSNEMLVKFDTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7Z5</accession>
    <name>A0A8J2T7Z5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S05-06040g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_06040g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF89976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7Z5"/>
    <dbReference type="OrthoDB" id="515064at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052145">
        <property type="entry name" value="Mediator/Homeobox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006939">
        <property type="entry name" value="SNF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24330">
        <property type="entry name" value="HOMEOBOX PROTEIN BARH-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04855">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="151"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="241"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="703"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="87"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="164"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="241"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="252"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="711"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="869" mass="97499" checksum="16DDAAEC7DD22DA6" modified="2022-05-25" version="1">MNGNGGAQPNSFGVGAASNASPGASPGRNPFSNIGTPSFSLSQIPRQILQSLAPAQIQMIQQRHQQLLMARVQQQQQQQQQQQSPQQQQQQQQQQQQQAQQQQQQQQQQQQQQQAQQQVRQIPGQRSPSANVQGATLAQRAAQFNQNASAAGNVATTAPSSGGPQQQQPPQHRPQQFLRNPGTNSVPVNLPPQIAKLPLQMQQHVLNTLKQQAIARNNPTVVAAVTLAQQQLQQQLEQQQLEQRQTQEAQQSQPSHPQKLHQPQSQQPEVHSNVGTPASVPYTNSPQSLPGRSSSQPARQSMGSMSMQIPTQQRVPPTQPKMQATPPETSAPPPPSTTTIPRMELPKFQTINYEPPEVRLPYPTYWSDQNPTTDTLLYEQIAQRDRAHKKDVIRETNGYEPFSVYGFSNKEYLGRLWHNLQYYQDLKSTRMKSITSTSQNVPTASIWGPGYSGYGNGITNKVTQVVSEVAVDDRPYIYQNRLQVYQQAMNEINEDLVPVRLEFDQERDRFFLRDTLLWNKNDRLIRIEEFVDDMMKDYRYAKHVRDQFADTVINSIKEQLLEYQPNPFRELNQERSGGDDMRIKIKIDIVVGQNQLVDQFEWDISNQENSPEEFAECMCQELSLPGEFMTAIAHSIREQVHMYHKSLSLLGYNFDGSVVEDDDIRSRILPVVTVDDVLRAPSDSKAYTPNLLQISAMELERLDKDKDRDTRRKRRQGRFNRRGVVLANGTSNQNGGILVSGGNNVTEVSLPDVSDIPRTFRTPVPSTILPGGVDLGPSVGSYELRTTTEYHQRPPKPPVEEPPCQIVDNVPGQFLLVSINLTERARNILKSYQNNASQQEDPEQSLSVTSAAASLPAADLSAAAAAEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T865</accession>
    <name>A0A8J2T865_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ZYBA0S05-03026g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_03026g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF89843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T865"/>
    <dbReference type="OrthoDB" id="5368485at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016445">
        <property type="entry name" value="Rog1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF20">
        <property type="entry name" value="LIPASE YDR444W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005412">
        <property type="entry name" value="UCP005412_abhydr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035175460" evidence="4">
        <location>
            <begin position="19"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="DUF676" evidence="5">
        <location>
            <begin position="221"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="459"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="526"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="465"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF89843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="689" mass="77688" checksum="360B1C21394D671B" modified="2022-05-25" version="1">MHVMMFVFVFAASHSFDALLEPNAKLKEATERVSQMGMLLCLDDDADDDGILLFADRQSVGMGDAWRYRITADKRELESNTKQLYLRIKNTESALLRPVYIAGPYSFYVDVRPCNYDENRKFDEPIQFCCDLRPDEHFCAKLLFNKNSQLNEHECSWTIDILSQMCVTPLPRVKFRLKIGTTKKCTKLRYGDDFKSSQGFQVQTWDTDKLWNAPPKFPARPVHLVIVTHGIFSNIGCDMLYLKERIEKAAFAVADEFNPNVIVRGCMDIMGKSSHGIRALGVKVGKFVIKIIDELNKHYKIDKLSFVGHSLGGPTQTMAIHYIALKRPDIFDPVTGIKPVNFIALASPFLGVASDVPMFVSAGLTVGTFGLTGRDLTLRHTPLTSKEGLATSDPNKGPTSPVMELLPLAPAREVLERFVHRTLYANVLHDGIVPLRTAALLYLDWRSLAQVRKMRRNFQHPNENSSSTSDLSSNLHSQSPLSSPMGSSANPKTGVIPESSLDKTAAAQFFMPHKLVKYRKYARIQTMSSPSEDSSDSEGNEPSPPKNINKEEAKFNPPPEASTILAAISVITSPLPTEEYIMNPEARTDCIVHDRLYYPNELPPPHYKDRPFYKKLIYPNETSNKLQEEIARSWQESMTWRKVLVDLKPDSHNNIVVRRRFVNLFGNVAINDLAESHFGLEASKRYATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8C9</accession>
    <name>A0A8J2T8C9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">alpha,alpha-trehalase</fullName>
            <ecNumber evidence="5">3.2.1.28</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_03796g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:32675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="3.2.1.28"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 65 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.28" evidence="5"/>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T8C9"/>
    <dbReference type="OrthoDB" id="200349at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004555">
        <property type="term" value="F:alpha,alpha-trehalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015976">
        <property type="term" value="P:carbon utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005993">
        <property type="term" value="P:trehalose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000032">
        <property type="entry name" value="Vacuolar acid trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.40">
        <property type="entry name" value="Glycoside hydrolase, family 65, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037018">
        <property type="entry name" value="GH65_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005194">
        <property type="entry name" value="Glyco_hydro_65_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005195">
        <property type="entry name" value="Glyco_hydro_65_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005196">
        <property type="entry name" value="Glyco_hydro_65_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11051">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11051:SF8">
        <property type="entry name" value="PROTEIN-GLUCOSYLGALACTOSYLHYDROXYLYSINE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03633">
        <property type="entry name" value="Glyco_hydro_65C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03632">
        <property type="entry name" value="Glyco_hydro_65m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03636">
        <property type="entry name" value="Glyco_hydro_65N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0574">Periplasm</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 65 N-terminal" evidence="14">
        <location>
            <begin position="117"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 65 central catalytic" evidence="12">
        <location>
            <begin position="443"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 65 C-terminal" evidence="13">
        <location>
            <begin position="815"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF90233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1175" mass="131903" checksum="8CFD1CCB43828C13" modified="2022-05-25" version="1">MKRDLTRNPSNVSNAAEHHAEEPKDSGIKTRLLGVSMVLFTIFTFFLLLGWPFGGLAPTFSAAESAGIIRKTSDVHTLDFDKEARRASKKLYELLVDIDGAFYDAESMVLGATKFSPHTYSRQPYVANGYIGSRIPNVGAGYAEDTWNIWANAGEELPGALNNGWPLRNHRFAGAFVSDFYCLEKTLNSTNFPELDENGYTTVISSIPQWTNLQFSLDETVWFNPQSINSDNVTNYRQELSMKNGVVTTHLDCFDEKIHVETQIFAHKRIYPLGIVSMKVSLNIDHLIPGQSSIELNFWDILDFNTSHRTVLQDLGYDDGIFMIVEPDNVPYSNASIFSTCTVDRTGVTFDHGETTYEQISQNASVILTTDYPNVTIHKYVGIVSSEYYNGSTLDYAKEIAKGNRGNYEKVLKVHNEAWDALYNDAFIEIPSDEVLEMTTRSSLYHLLANTRTYNVSEARGLPVPVSGLSSDSYGGMVFWDADFWIQPSVLPFFPDIAKNINNYRNATHRQAQLNAQQHGYPGAIYPWTSGRYANCTSTGPCMDYEYHINVDIALASFSIYLNGAQGIDDTYLKYTTWPIVRDAAEFFTEFVKYNHTIGKYETHNLTDPDEFANHINNGAFTNAGIRLLLKWATDIGHHLREPVDPRWMNISENIYIPISESNITLEYSGMNSSVEIKQADVTLMVYPLGYITDELVLNNAIKDLYYYSEHQSSSGPAMTYPVFVASAAGMLNHGSSSQSYLYKSLLPYLRAPFAQFSEQSDDNFLTNGKTQPAFPFLTANGGFLQSVLFGLTGIRYTYEVNPASKKIERVLKFNPIELPLLPGGIAIRNFKYMNQVLDIIIDDHNGTIVHKSGDKPIRIKVPDRSLIRDRDVNPYRGTNSSEEVVNEKGHDIEALGDKSLARKAQHYTLKPGEELILPLFKMKKNVEGNVVESKQITNLTGGVPGDVAFSALDGNNYTHWQPVDKSVPAKLLIDLGQNNSQVIQKGMILWGQRPAKNITISILPHTETVGQILEELTTIAENVNVGEESVMEALEDVGGTDFLLANHGDQDVKDLLSWRAEDLEEIVRQLSNDSNVMRGKFVPILENYSVVPTVPYFKEIYDKSIIQILPSNITEFTIDYSKITHNSSRCSYRNSLNNCTDWKKTRFILVGIQGSYDEDLDPKGGTIKEIVLMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8J1</accession>
    <name>A0A8J2T8J1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component SEC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_12420g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SEC5 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T8J1"/>
    <dbReference type="OrthoDB" id="26242at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029175">
        <property type="entry name" value="EXOC2/Sec5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039481">
        <property type="entry name" value="EXOC2/Sec5_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15469">
        <property type="entry name" value="Sec5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC2/Sec5 N-terminal" evidence="5">
        <location>
            <begin position="82"/>
            <end position="933"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF15469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF89236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="934" mass="108564" checksum="CC2D60195B580CEF" modified="2022-05-25" version="1">MDPFGASEEQLLEFYHLKTLEPHTSWKQDSSLDFNLERWQNVSPDADNSYDILKDLLYQQQQTYEREVAMNMNDAAMSNVADPLNNKMMLPLLNKLHIPQKEKLKYLVNTKKFNVKMFLKDIHNKDSFDDLSRSLDILDKTLESQSEELKDLVQNNFTKYVRIKNRLDQIYEQFSMRYTPTTEALDNNRGQLDVNELGEKVDDAIRATTLKLKPLLETNRKINNYQATRNFIEENKEYFNLTKKLKRCLDKKDYSSLIIEYSKAKELHNQLILDAAVTEKNESGQIVNRVPKVVDKVWDEVERIVDNYRQYTWKMLLSPDEEESQNSFLPLISKLLDLTVDKNPIMAWLTVHLDHFEKRLQDVSSQMLNKIVKAQNNILQNKVETDSDEQQTASIKGVDLSYYLSIGRFFHDNTELKSNDELAFRTLSAFQGLTDSPVVVEMWLLFLRYINSLEKLCMKFVEFWEHVQNFLDGVYQITLINDKKKDNILVGDLKTIDGHKQFLQLEKEQIKHLRNRGEHFVNMLSNQLTLFFMSSQDSLSGGAMISKETGLPSDYGFTPPMTNGLSCLRYLPKMVEPLLKSVTKLAQLGISSKALEISRKLVSMITDRSVGAIAATKLRDTANFYKLEDWKVYETVTEANKNKLEYGVTQFPEIVRVFQEYSIQTTRDLLFAFEKLPVFNGISIVTYPSKRSLTAVEIQQLISMEAVLEAILKNAAKDKDNPRNSHTILTLTNLQYIRETTFPQILQYFDEAFEWNLREKKLELFSLLSKMESSIFGNYLSDLKINIRDVLEKKFHEISWATYTSNSFRAHDYVIEVLMLLVTIHSECFRIGPQLIEKVLKETQIFIAKFLFESFKPYIGNLSSDGLLQVTVDLQFFQRVLGKLLERDTEVTITACLQNCFQNNVERMQRCIKETEPIVSSNLARTSIQFAAFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8U8</accession>
    <name>A0A8J2T8U8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_06128g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T8U8"/>
    <dbReference type="OrthoDB" id="68483at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900180">
        <property type="term" value="P:regulation of protein localization to nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14008">
        <property type="entry name" value="STKc_LKB1_CaMKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000206">
        <property type="entry name" value="Serine/threonine-protein kinase Ssp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000829">
        <property type="entry name" value="Serine/threonine-protein kinase TOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF152">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE TOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="117"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="21"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="701"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="886"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="989"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1030"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="805"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="893"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="989"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1055"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1081"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1111"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF90334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1120" mass="125086" checksum="F532799C118580DB" modified="2022-05-25" version="1">MDSQPDSVEEVDVPADLQLVLNQSARNDSSLSLENLQNGSSHENSSQNTEEELSESSSVESLDLLLEKQRVRQLNHPRHQEHIISPNNLELPDKVIETNEISLKYDPVSKRKVLNTYELIQELGHGQHGKVKLARELVTNQLVAIKIVDRHEKNGRRFFSIEKKNSLTQNEKIRREIAIMKKCHYKHVVRLLEVLDDLKSRKIYLVLEYCARGEVKWCPGDVMETEARGPPLLNFQRTREIIRGVVLGLEYLHYQGVIHRDIKPANLLIAGDGTVKISDFGVSLAARGPNDNANVESLDELELAKTAGTPVFFAPEICLGDEAFEKFNLDRKELFKGSSISFMIDIWALGVTLYCLLFGMLPFISDFELELFEKIVNQPLTCPTFQDIKHNRISHVSCEEEYQLALDLLNGLLQKNPLHRIGIPGIKKHMFSCWDFHHMPDYGDEFKAYKKREQEEFERNNVQYFKQISISKKELNNAVLGVGKKIKDAAFSRNRRDSQQNGKEEMDELGRDFRSKLHLGDSGESNYPSFIVSEGSVMSDFGNLKSKSQPPSNDKDNAIVTNVNPSHLRERLSMCNGNDDKYNDKTAEPPHTPAIERELQDFDRRQHNSDTVVNLPINSSFASLDSFYIDNFAMKKVGTNPNVKPASPTIFARPPPMGSYQPISRTAGNADQNKISNNNSPLYQNAPHTNFFNGMRSKRNERIPSNFSRSGQALEKRKPEMTGKGLKTAPIKGIASNSNEKKNGRNATMGIFKDAPVNHPMKKDAKNFINPTSGSVRTREGTRNFHIRTGNFFNSLNGEDEEEFSQSSGMSSSGSSDHEDASESSNAESLPFEFAVDSGNASVISLRDTVGLDQVRSFLEPAPRKIAVNSDLENDDGELVLNMGRASHHRRQLSAQTSEGSEQNSRRSSVRCAFLTPNSNPQDWTATATPEALQDGGTESGSVVDNDRATFIGRNRAHSVTQIGQSSANIGVVQHYNPFNGKESLRLSQALSKPTTQTSNIDTVLGNGNGSGNFHDNSRSLLKNVLVTSAGSSRRPSAPFLVSSEPFSPRKDTSDMSPSSPKSSPSTDLKDNLTAKLSKFSFKERKPRQSERSSNPSDDHHRSRSIAVGDLDNKKRNGKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9H6</accession>
    <name>A0A8J2T9H6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ZYBA0S10-04368g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_04368g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316463">
        <property type="protein sequence ID" value="CDF91307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T9H6"/>
    <dbReference type="OrthoDB" id="760868at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000244">
        <property type="entry name" value="Nonsense-mediated mRNA decay protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF18">
        <property type="entry name" value="D-IMPORTIN 7_RANBP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="7">
        <location>
            <begin position="24"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF91307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1021" mass="116068" checksum="5614038237F57662" modified="2022-05-25" version="1">MDVGGLLLCFAGTLDQNSQTRSNAELQLKQVSSTPGFLGACLDIIAAEPTPENIRLSASLYFKNKCVNGWNGKYTGKNDLLNYVVDQDEKPVIKDMLIHTLVACVKASPRCARVLKSALGVIVYTEFSEGHWDDLLTRSFKSLAGHDLDEAYVGLLCLSEIFRTYRWKENDSRQGLEVLILQYFPDLLRYGTEQLLKGGAKVSDVKRGEMLLLLIKIYKFVTYLDLPFTLQRSEAFIPWANFFVAIIQMPLTADFVTGNDHDSRSKNPWVKCKKWSYAILYRLYQRFASESLTRKFEYEEFRNLYRNEFLPQLLQLLFQQLEQWSSGALWLSDECLYYILSFIEQTIVQKPTWKLVKPHYATILEHVIFPMLCPNDEKLEIFENDPQEYIHRNLELWDENYSPDLAAVSVLTTSVTKHGKYTLQPTVEFVIATLQANVGSSASLPIAVKVESAFRIFSSIVDRLTTEDSPYLSELETFLNTFVFPFFSSPFGFLRTRVCEICSKLGAISFQKSTLVDTIYGGIMVCLNDSNGCLPAQLLAALALQAFIHVPEFQTALSPVVLPIMQKLLDISNEFESDTISGVMQDFVEQFADQLQPFGVDLMNTLVQQFLKLAIELHEASNVDPNSLLDDNDIPDESDKQMAALGILSTTISILLSFENSPEMLKNLEQSFYPAAEFILKYEIEDFYRECCEFVENSTFLLRNITPISWKILELIGESNRKQDSMVSFYLEDCIYALNNYLLYGSDELRKNEFYSRILFEIYDKAVNDADKTLSELATVLDLSQKMILSLNHSLTDAQKRRLINDATHAIVVKQEELRENIVFGVTSFNVIASGLAYFPTLTLEILKTYDCIELFFKTWLTFYVPNVKRVYDIKLSILALLNIITEVPDKALATVLGGDIIPRLGKTTVDLISRFPSAQRLLQEKRMGFSTGAFSLDEPNDWKNDSGTLKFVNGETFDAAEDFEDLEEDSLSGSLLDPIDVYASVKIFAINLQTTNPEKYTRVGSEMSPDDQVLFNRIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9N7</accession>
    <name>A0A8J2T9N7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SAGA complex subunit Spt7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_01992g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316465">
        <property type="protein sequence ID" value="CDF91571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T9N7"/>
    <dbReference type="OrthoDB" id="21449at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05510">
        <property type="entry name" value="Bromo_SPT7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22927">
        <property type="entry name" value="HFD_SPT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000032">
        <property type="entry name" value="Transcriptional activator spt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037782">
        <property type="entry name" value="Spt7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47343">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR SPT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47343:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR SPT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="424"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="84"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="190"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="299"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="527"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1154"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1219"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="105"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="209"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="527"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="622"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="650"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1154"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1237"/>
            <end position="1260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF91571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1267" mass="144247" checksum="C6C8CC850FF2E091" modified="2022-05-25" version="1">MDGKVPIVNYQQTNTLSLLQLTEKLKNAQFFESYLTSQQRIVLDTLLAIPDPDTKFKVWHELMQGNATLDVEKVEVIEEQLLRQADNQNGTDPHDIGDVIEKPDSSNIENEANVASSGPDNQEEEDFSELDLENFKQQIIGVEFIGNLSLKIRYVLWQCAIDSLYTNEGDEFNQEGKDIFDYILLDDHTSQEKEKHSTEGQAPTLTENREEDEDYDDEDEDFGGDDKSKVPGPQSRHSVEVRTKENGRVELVLKISKDVLSKLRTDNVEQIMQNCSKIYHNFEHDKETMLKRLKLDETEMLDPSKKRSHSDMDRVAGEENGNQQQDGALHEDTMSSTEETISDGKRPRHDSMNIPVSLGAANLSLKHLLSSIQENKHKLRITDYELKHLIMDVRKNRSKWASDARIGQEELYEACEKVVLELRNYTEHSTPFLNKVSKREAPNYHQIIKKSMDLNTVLKKLKTFQYNSKQEFVEDIMLIWKNCLTYNSDPSHFLRGHAIAMQKKSMQLIPLIPNIVVRNRADVEKEFEEMERDKDYEEDEEVAGSGRKGLNMGAHKPAKNHDAQEASKGGGKHPVESSVNSTQGAAQKELSKQEQLTPATDAKLNESEEASKAATPAETDGAKSSKSLANGLPQNDVINSISPDKKNGGNEDEEEDEEEEEEEEEMADSQAYLVEKDDDRDDVEISVWKSLTAKVRAEICFRRSQYFKDGRLNKESRALLKDPQKMKSFEQLYDEYKEQKALELQTKQMEQESIMKNGFRTAIKQEDEDQLPYQESALDDSEPMDKESNDIELDNGMFLQEYDSSNVLPGLAYYGIDSTFLDKEEDAWTKRMLEDKDPKPSIFMSNRNKGLTPKVNKNIELIQQIRHLCHKISLIRLLQNPTYSQSSKFTGNPNQFVNTHQYKYHIIDDSIDLDPVSQLPTHDYKANKELMWKLMYRNVGKIAMSNGFETTQPSAISMLTEISGDYLSNLIKSVKLHQESNSLNHTAAKEILHMSLLENGINKPDDLYTYIEAEFTKKTKKLTDVKGKLENFLRDLLRPTLQELSERNFDDESQSFVTGDFASEITGEDFFGFRELGLEREFGVLSSAVPLHLLSFQFQATDSEDKVQIKKIQPEEFDSVGYKKLKKQDIDEESCVGIIRPLLKLSLERSTAYRSKNAKSNNVSDQIAPNVTSEDDKNLLLEDDEMVLKAKTGSKPRVPPTGKISISNKKKAVGDMFVLPETEDKEEKSTPAAGAKSSVNTPQNVPASEIDTSSNTTFNLSIPRLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9P8</accession>
    <name>A0A8J2T9P8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S05-06898g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_06898g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T9P8"/>
    <dbReference type="OrthoDB" id="410044at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12453">
        <property type="entry name" value="RRM1_RIM4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034352">
        <property type="entry name" value="Rim4_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21245">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="164"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="435"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="125"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="344"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="512"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="619"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="784"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="348"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="362"/>
            <end position="375"/>
        </location>
    </feature>
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        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
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        <location>
            <begin position="406"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="526"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="625"/>
            <end position="636"/>
        </location>
    </feature>
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        <location>
            <begin position="637"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="656"/>
            <end position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF90014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="812" mass="88956" checksum="6978DFE011105F99" modified="2022-05-25" version="1">MTIASVMDPVAGAEHPSMDTEIGGAIQHSKGDTIISEDIGQLLPTEVSSAESDNFDDDEENEVDNENENLLDDITEEDIQQSVAIELNSQSPSHPEDQNVQKQEQLLPTDHEKDTPVGVECNATASKQHEHATDNKPSTSSAIAERNSKKDDSASTSFRGRPSSCVFVASLAAALSDDELCISVTQSFKQYGQLARVKVLRDPANRPYAFVQYTNDKDAKRALKMARGSVLNGRTLRCESARVNRTLFVTHVTPIYFVEVANLCEAFGELEQLVPSNDNNQLSRRCTYPPSNNNSWFVQFAYRDDAIRAFANLRTTSSWDVEWVQNIEVPKYFNLLNKRKDNNKSTDSRFNIKNVTTSNSDTKNDGEKNGDHSEGDDNDSDSDDNDEDYEDGDESEAGSSYSNEKNYSHGRGEDNMGRKSVGSSRMDDNVLIDKHSIFVGQLDQSVTKERLNERFSTHGSISDINLISKINKVFAFIQFETEEAAAAALERENHAIFLNKTMHVQYKEIGGHRGRRQFRKNSNGNGFYGTGSGRNGGGSGRMFTGPQVNLAPPPINMYRRRSQQGDSLACSYMGPPMAGPTTDFEMNYVPYMNGQFRRKSFANGWSSSRSHSMKSEIDTICGDGATDATSEASESAGQTNSATTYNNSSAGSMGHTTTTNASNNNSGNQTSHGNHHNNSGSNGNGANNHFNGSGSKKRYGKRGNNGGFNDAPKPYYFQPYYYHPMHYPMGPMGPVAPAHPSQAPGGNHPYMMIYPMPPPPPSGVDGNLLAQNLPVGLPGSVLHGSPPSAQSQGMHCDSNEFTPNAKPCQLDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9R9</accession>
    <name>A0A8J2T9R9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Man(5)GlcNAc(2)-PP-dolichol translocation protein RFT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_07690g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Intramembrane glycolipid transporter that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. RFT1 could mediate the translocation of the cytosolically oriented intermediate DolPP-GlcNAc2Man5, produced by ALG11, into the ER lumen where dolichol-linked oligosaccharides assembly continues. However, the intramembrane lipid transporter activity could not be confirmed in vitro.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the RFT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T9R9"/>
    <dbReference type="OrthoDB" id="9979195at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034203">
        <property type="term" value="P:glycolipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007594">
        <property type="entry name" value="RFT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13117">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MULTISPAN TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13117:SF5">
        <property type="entry name" value="PROTEIN RFT1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04506">
        <property type="entry name" value="Rft-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="352"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="446"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="490"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="515"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF90044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="552" mass="63350" checksum="B0C7A508CDE2598D" modified="2022-05-25" version="1">MEKKVAESPSGQQMLEKSTKGATFLMMGQLVTKMTTFVLNSLLVRFLSPKIFGITAFLEFIIGTVLFFSREAVRLSTLRIKDSSEDEKGRSRSDVLQTAVNFAHIPIWIGAPLSIVLTSWQYRNLNSYFIALPFFTWSMLLIWSSIIIELLSEPFFIANQFMLNYGTRSQIESISVTFGCVVNFMVIMGFEKWFGNSGEDSETMREGIAILAFALGKVSHSVMLLLCYFYDYQKHFASERLFAVRLTKIYPPHEKQGYYFQEEISQHFKKVYSQLCFKHLLTEGDKLIINSLCSVEEQGIYSLLSNYGSLLTRLLFAPIEESLRLFLARLLSSGDKKNLKLSMRVLENLTKFYSYLSLMIIIFGPTNSSFLLQFLVGSKWSTTSVLDTIRVYCFYLPFLALNGIFEAFFQSIATGDQILKHSYFMMTFSGVFLLSCWILIEYLNLSINGLIISNIINMILRMTYCGVFIQRFSRALHSGSSRFDPRKFRTVFLLGLLVWVLDWGLIGFVKNFRQFFLNVILASIMLVVILFKEKGALGELIGERKFSRTKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9V2</accession>
    <name>A0A8J2T9V2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ZYBA0S06-00738g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_00738g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T9V2"/>
    <dbReference type="OrthoDB" id="4159781at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000014">
        <property type="entry name" value="HAP1p Zinc finger transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051430">
        <property type="entry name" value="Fungal_TF_Env_Response"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31944">
        <property type="entry name" value="HEME-RESPONSIVE ZINC FINGER TRANSCRIPTION FACTOR HAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31944:SF131">
        <property type="entry name" value="HEME-RESPONSIVE ZINC FINGER TRANSCRIPTION FACTOR HAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="28"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="98"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="269"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="897"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="979"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="111"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="269"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="294"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="980"/>
            <end position="992"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF90108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1251" mass="139023" checksum="E245BF3D83C2308B" modified="2022-05-25" version="1">MSAESSVLSGNNPTSGKLKRKRNRIPLSCTICRKRKVKCDKTRPNCEQCSKTGVAHLCHYMEQTWAEEAEKEISKDSELKKLKNRVRVLEEALVKAQSSYSHTPDSESPFLEQNTPQAGPNVSSSGRHDNDELDLTRQFDMLHLRNNGTIHLGATHWLAIMKGDPYLKLLWGHIFTMKEKLAEWYNQKKQNPHTSNGRCPVMPHAPKGGCPVDHKGMKTMAAKWQSPQGGVGKCPVDHNALKKCPVEQSAAKKCPVDHSALKKCPVNHSTMSRPFLSSSPNLASQYPMKREENPTASSRSRQPSLPSIQNLTSKCPVLHDALPGIQDAVPTIKGAPKEPVAEHKLNHEQVKSRICELLPPKRIICMFLEKFFKQIYPVIPILDESTFKNHMNYMLSLSVNTETENVTHIRMNKLADFCNLGILMIILRLTWLSLPINGCKIDLGSQCTSFFMPHATTASATQAREEASLVNYETPAEALGLVRENLIKFEQISSISNSNVNLTTVQFAIFYKLYLMCWSNDSSTQNANGDQFPSAAGDNQDNETHQVLLSSITQMAFSCGLHRDPDNFAQLTAVPYMHVDTTSTCNNGANAAGLARPNKDAIASAERFKHAWRKTWYYIVSLDVQQSLSLGTPRLLRNLKDFSDTKLPSTSKLDYVNDIKELVIVKNTTLFFQIDLCIIAVLNHVLNVSVAKSVKKFQLDLLINSLSDLTHNNKNICEVLNGLINSGLLHSTEGAVDSDPDNIYGLPSLKEIISQQSVSSSENDVDRRLDLAYESATKALFFSKHLTLRMLLYLLNYILFTHYEPMGVENPDTASIAKSYAQETLNYAMDGYRNCIFFFTNVGQPGSSSSMFNYMEVVLAPQCLDIGHRALQFIVCLILRAKCGPLTGMGESAIIGNNGASSNSEEETEQNASAKKEASVDQIHDINEEISLDTGELLADTLFSKMVLFHKMTRQICVKYHYALRTMKSTGFFISLLRDPNQKGKRDPKNDSRSTGQKRSRFTNMTGLFKNVPSLILSGDGEQLKRCPVYQDAIGFMAPRVGSHNTSSEDSARSEIQLPPLKPYKPITYISSSVRTESQNEASKRRRLNYGSPLKTADLAYGPLPSVHPVAPQKDSTDNYETLPPLERITGKLTPIQTANRIPPLNTSEVPYSVNAPMQMGIDNTSVSAFTSPASENSLGDLKPSFEDFLTQNANFNGIMINPSSIVEAVGFQNYDMQLPMTSDAVDFLPLDSTGIDELAEANGTQFPSWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9Y5</accession>
    <name>A0A8J2T9Y5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Actin cytoskeleton-regulatory complex protein PAN1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Actin cytoskeleton-regulatory complex protein pan1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">BN860_02388g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PAN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316466">
        <property type="protein sequence ID" value="CDF91736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2015333at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
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        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
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        </source>
    </evidence>
    <sequence length="1068" mass="118141" checksum="06AA1A8B49FE9F9D" modified="2022-05-25" version="1">MSSSEEDLGTQLKGLNIVNLGETTKPPESTVGKGSRKNSVDLGSVSYRAYGNDEMTEIHYRDSEGAGGKAAAFDRGDDVEESGSNNDSDSDDGWQTMPAIASYEVYDDKGQLSVCPYEYSDLRSHSQEALASSTSSLFKDKKGDPKSSAKTTFGYTKVAAEEQAQRSLQSNRKTDFLFDHRKLLADSSKGSSNTSRSSNTSDEDKSNGEVDDEATYYDEYEDDVEPMDDLNQDTQLDVTKNLLTEKEKFAYVGAVNVLLNQMCTELATLCLCVDNITSHKKLARRLHVAQRDAAFWKTDILGRLYSHLDLSEDEIKMIEQLSHHGIGLQDLCKCLKTQQNVENPFEGATGETTNASGATGVHLDSKYEEKEKKKDVDAKEREEQAAKKVEVDVAGSSGESLTSSITKRNLEDDNEEKEPYNNIPNEVVNPETIKDKSQIEIDVAWTVICDLFLVLLQDAIYDSRSRILLLRFAQLLNVTNLEVCEFERRVTDSLDMEQSTEDQQWTESQHMKDRRRRRRRKKICYVGLAMVGGSLVLGLSGGLLAPVIGAGIAAGLSTIGITGAAGFLTGAGGATIVAVTSTAIGGKIGLQGMKRRMGSVRTFEFCPLHNNRRVNLLVSVSGWMIGNEDDVRLPYSTVDPVEGDLYSLNWEPEMLKSIGQSITIIANELFTTTIQQVLGATVLTAFISAIQWPMWLSKLSYILDNPWNVSLDRAWSAGLILADTLIARNLGQRPTTLMGFSLGSRVIFSCLVELCKRKAYGLVENVYLFGTPVIKNKEYLVMARSVVSGRFVNAYSSKDWMLAYLFRAAAGGFSTVMGIAPINHVEGIENFDCTDVVDGHLAYRKNMPKLLKRLGIAVVSEKFVEIEEDVDPEEIERKRKLVEDVDAAQRKLSEKKKNNSWMPKWLKPKKSKWRSMVENAVEEGNEIEAPPLPPKEGLEPDPSKEPAPEEPTKPIKKDPTLVDHGALMHELKVIKEAIHEEQRKNEAEAAAAAAAEARTANALGSGISTEDGNIPFKSAPQTPQTPQTPKTTNNFQLLSAGRTILPEDDDMRRGKSRNDMTFEFPDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TA78</accession>
    <name>A0A8J2TA78_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ZYBA0S08-01706g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_01706g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316461">
        <property type="protein sequence ID" value="CDF90733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2149224at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032065">
        <property type="term" value="P:maintenance of protein location in cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053005">
        <property type="entry name" value="Nuclear_Pos-Cytoskel_Interact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024774">
        <property type="entry name" value="PH_dom-Mcp5-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28190">
        <property type="entry name" value="NUCLEAR MIGRATION PROTEIN NUM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28190:SF1">
        <property type="entry name" value="NUCLEAR MIGRATION PROTEIN NUM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12814">
        <property type="entry name" value="Mcp5_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="2322"/>
            <end position="2430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="369"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1560"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2105"/>
            <end position="2135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2220"/>
            <end position="2298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1862"/>
            <end position="1913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="369"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1572"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2111"/>
            <end position="2121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2224"/>
            <end position="2245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2259"/>
            <end position="2268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF90733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2478" mass="280668" checksum="524D8A954FD695AB" modified="2022-05-25" version="1">MSSNSNKRESLLTQLENMHKQLNDESEAKAGQSNATDVTQGAGIKKPNLFSNVLNRREKANPMPMFAGSAAHNGVLTANNGKDMSFVMGLSENLLVECRRLQAENEKKTKKLKQVQEEFEQVDEKHKKLDSFHQLTTKELNALRDTNWELEVKVQQFTVELRELKDKLGKNRKELKTEQENSQNTKAELEVAVLQRSGLEKELSQREKEHSSELTDLKKQISELNDENDSLYTKLQTLDEISIDRKALQVELSQSNKLTDQLKQQIVHLETRNKDYAENFTASQKQISQSNVVIDQLKQQVLNLEAEGKMTINAKVIESELSQSRNEVIKLREQLVSLEAKQTSYPQDLQLELSNSKQLVEDLRQQLKKLKGNKSGKGKSKVQKNKNDSTHSENIKEASGDEKEVINSRDSAIMAEGSERGDVHYDISEFSLTELEKYAKLYNMILVPETEYTTTNSKDSKKTDSQEQLVNTVGDTNLLALPNDNSSKKKSVDDSTSIESMIKKLESSGYRVLSFPEFNEKQEQLKELEDPSETFLMSKASHLHKILISQDSYDKLANPTLSQLSSKLEELGFTIMKIDEHEKLLESIEKPNSKYLEEKLALNNKVAISKEEYKQYTSPNEKFIKDHAKAIGYVAVIKESYETMKKQVTQPSIETILKNLEKDDYRGIISQFCAKHHLVLLEKSKHDQLISSINNPSRKYLEEKCPSQQIQPVPVDKYNKLLQDLKNPTLDFMNEKAASMNKKLIPIKTYQKLQSTSEKPTLEFIKENAKKSGFILLSESDYISMKKMLDEPDLEYLQSRAKHHKLVDLDTYRLLKQNFDNPPLEFLTQKASAKDYDLVGTNTHKELLRKAMHPSEAEVKHFAEGLGAVLVQVHEYESLKAAENSPSKDFIVEKAKKLNLVVMSEKEYQKSQKIASDKNYLLSSIKQFGFIPVPAPELITLRGSTLENSSLDNIEKKLQSLGYVAVPYERFKELNRPLTERATKEETLLLCSKYSFKPVSLGEYQKLKDDSKPTVYSEDELVGALKSRGYVVLAEDEYNDMKGLIASPTAEFLQKHAATQGSIIIARKEHEKQVNMIAQPSLAYLKEKTSVLGYRIESEKELDKLHKQLDSPSSDYLQQKAPLLNMKIVSQEKYQKMMNEINNPSLSYLQSKAAAMKKQVIDGAEYRELQRKTTFPTLEEIEAYAVTLNMVLVVKDEQENLLKQVNHPTLDYLKQSLEQYDAIPVALPEYNSLRKNVEEPTTDYLSDKAASRDMLIVSKAEYNKQMERLIDPSEKDVIEDAKKFSKILASPSDLKTTEEAHLVEDIEKGNSVVISRNELIELKQAVKNPSEDTMKSGLEKLNYVVRQSREFEELIRRVETPSLDEIANSAKSQGYVLVRAQDLAKHTTVDTEDLDSLTQSGSYVLLNADEYDGLLKSSAGKMSKREIMAICKKFELIPVLSSEYEQFTVPPDVNKIKQYALDNDMTVISCKDFDLLKAKAEIPLEQSVKKFTEEQNLMLLEKSSYDRLLNKISNPSKEELKKQAGKIGLAVIPADQYNIFVERTNKQQLFVQNSYDEKENRRSLASHKSNENSETSRVITGGYDTTSSGVVPLNLAQSNIYGAAEKLGLTILSTEEYKNLLSKKFVTDGTSYNELEELAKKFAMKLVPLKSPADDKSSTSASQLRLTIADESRNALIKTRSSNSTIVSMNTTYYDADEENQADKFIACNKEIQEKTYTQVDDHSQASLPEKTIPSDTSLETSVIKRSETLKDDDLKQNAAAEGFVLVSAQEYDEILKLKGKMKSSSILHAEDDVSWYDASDFGDDINEETALLAKRAGRLGQSLLPTPVYQEYLHYKNATTKEMLEEQATQLGLIVLEEEAFNELTLKADESKDRMKDLANKYGYHMMSGEEYTNLKEQSQNAEIDSANIQSKAQELGYHVLTEKEYEDLKETLCEGTRKLTKEELQEKAAEYGLVTLPMKAYEELTSSGHARKPLKITRDELEERASEMGFTAVPNDRYKELVCSRATQELPRGELEKRASQLNLVILSTSEYKRLTSKPNVTKEVITSRAKEYGLTMVPTKTYDALTVQNLSMNSKEGLSSHAQKLGLRVLDEEQYGDLLNNTKHQGVSKKDAERKNSVKEIQPAKKNTGSSLSKDDIVNACGKFGMVAISKKEHVRLHNLAKTESLKQRAQEANMICLPRSTMVASPASYSSKQEDDVVVLPTAYYKKLLSKEISTISKRKQQERDMKPAQKPLQKPEHEMRFVQATQKPKEKRSAASLRSTASRSGKRDLAGPRSATAFPAAAHNEPSAIPRDKSMGALSLNTIESLDDPSIIPALTQVVIGEYLHKYYQVLGLGQESRHERYFWVHPYTLTLYWSPTNPVLESPANHRARCASIVGVDSVNDKNASQGIYHKILVITTDTRVIKIACPSRQRHNVWYNSLRYLMQRSMDGINLEDIAENPGDTMYSGKIYPLSKHTSQKLPRSKSMSLLKGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAI7</accession>
    <name>A0A8J2TAI7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ZYBA0S09-00694g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_00694g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP12 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316462">
        <property type="protein sequence ID" value="CDF90919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2TAI7"/>
    <dbReference type="OrthoDB" id="2192888at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012978">
        <property type="entry name" value="HEAT_RRP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057860">
        <property type="entry name" value="HEAT_RRP12_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052087">
        <property type="entry name" value="RRP12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48287">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48287:SF1">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25772">
        <property type="entry name" value="HEAT_RRP12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08161">
        <property type="entry name" value="RRP12_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRP12 N-terminal HEAT" evidence="6">
        <location>
            <begin position="24"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="RRP12 HEAT" evidence="5">
        <location>
            <begin position="363"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1018"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1160"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1161"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1184"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1198"/>
            <end position="1215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF90919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1215" mass="134998" checksum="5B7163A62020EE69" modified="2022-05-25" version="1">MDSTHEGSELGMEDRLNKIRHQINSKLENQKHVAIVLSAVEENLAGTSGKATSPVHYTVALISLLDQASDPETRAVTDLQLATSACYLLDISLRFTPTPLLRERFAELLTKVAPCITDENAEAPLIRAAIGCLEMLLINQHAQSWANTEDLNITPRRGLNGLLELSLDPRPKVRKRSQEAVYAVLAHAAPGSAAGRVISTTIVAFILKELSSGLQEASSFAKKKKQGLENDDLHARIMHTLRLTSTILSTGQWPTFQTEQLCDLLLEVTKSSDQYLVSATFECFENLFKSLADSSDTSDLAEGNSLKVLDIILSLKPSSTDIHLAGSWIAVIVKGMSAYAARKPLQSLLKVPELFRVMSHFLSSESPEVYISASHCLIAILSETISDKLLLYPPAVDGTTFEAVDETISELAEIFTEFLSVRYSHCARDILKVLAAAFRKLRYRANPDFIKPLEIVGEWRTNEESFLEFRNEAESVIGASIASMGTDVVLSCLPLNLDNPSVERPGRAWLLPLIRDNTRNTKLSTFIKVLAPISQLYESKFEALAKDSVQLKIFQTVVDQIWSTLPHFCELPTDLRTSFTSDFASDLSSMMYAKVESRTTICNALKVLVESNLLHVEGALAEDFMLQQQLPMSEAKKNLEWLSTMASNLLAVLFNVYTQTASNARAFILETIEAYLKVTSEVDLEKTFNNVCGLLKNAMDKDSSQAKGNKPQLTATLLDLVVCMSKYLPTTSYPALFTIFGSTVVSPDALIQKRAYRIVTRLAELPSGSDALSKFMPDIESIILDNSSTVQTASKSARLGAIKSLIELLPFDHMDFIVRIVAEVILSTKNVNEKSRDIGFECLIAMGKRMDEPNSVIRFSKIPGYEENAPDQISSVSEFFKVIAAGLIGESQHMVSSTIQAFACLIFEFRDIVEPAVLLDVYDTVALYLTSNSREIAKSAIGFAKVSVLSLPTELVRPKVPELLPKLLRWSHEHTGHFKAKVKHIIERLIRKFGYDYIASNFPQEDLRLLANIRKVKNRSKRGKGTDEQAGPTDGSTSNRGSRFMNAFDEALYDSSEEEDVSDEEKVNHKNKSKQFIMESKENPLDLLDSQTLAHISSTRPKKFNKADRRKHLDDEMFSFDAEGKLVMNDNRNSEEDPLKSVSSGIDAYVDAVKQGPVKGQRNRLKYKRNARAGAADSDDEDGDVLRLPKKTFDTKNRIGKGGKKGGKFKSRRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAJ3</accession>
    <name>A0A8J2TAJ3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide exchange factor LTE1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_04390g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the LTE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10254377at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000019">
        <property type="entry name" value="Guanine nucleotide exchange factor LTE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF363">
        <property type="entry name" value="PROTEIN SON OF SEVENLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="13">
        <location>
            <begin position="28"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="12">
        <location>
            <begin position="1188"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="340"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="623"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="697"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="857"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="994"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1039"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="344"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="629"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="740"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="755"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="857"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="882"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="896"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="929"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="995"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1047"/>
            <end position="1059"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF90582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1435" mass="162253" checksum="7BBEEE4D63902A05" modified="2022-05-25" version="1">MDIFSRPDYYPSPSSNVVKYREPDGFKGKRTILNADLVALVVDLSSPVDAVDYTVISDFFLIYRNFMSPMQLQHLLVMRFRWCISEIEVETSKTNRRRKIGEIALVRTFVLLRHWILNYFVQDFLVDVNLRIQLIKFLNRKLKTYPKIVRCTVLNLKKAWIHCSKRVWTNLDLDEPKIIGEDDLWVKYEIKDITELEELKKRNSQLSSYAIQGSSSPNFRNQSVLSLYKSTDVFQLPVPSAAERRKKDNRTASMILFPQDNSNVKVIDTRKPLNAWSAADSKAEHEGNGLKGHNKISRLSKLTKVSTVMKDIDYPSSPGINKVMPPTPAKKVELILQLPDDNKNNSTNSTLPFSRNGSVRRSSSLGRISSVGRSSNSRNINTIGRSASTGRAGSVSRSSNFRSSMMHHGAVGLMSKWKKNHTYANRHDNEASLKKKNDNIKPEMENFIKYVISISSLEDRNFAPEEMENLISTKFDLLSARTIEEVEYLVTLENNLIKEVNQTVTEDEKPPIVQETEKEKQVSDEMEFSAMDNLNIYQTVNTIANSVFSLSKAFNQHKQQQIQPQNIVASPSFAVLERRQVQSAMPFAGAHSHSRLSMNSQNIEKECTPSFRDNGPQRLIFHGSSIQRDPTKEKDAHRFSDTSSLMTPTKLRRSPSPKRTCPSPSKQPLPDVQECHDLSSDGVHNDNASTLSCVSYDSKLSNPENLPIGTGRRSRKVSLTYPNQDHSLKRKDAQPNLREFTFEDKERLEKEDTSSLDSFVTTNEPESDGSEKASFHEASEFTATIVRQSSGRVSLSGGRTRIYSNARESMWSAGQRVSQDPDFLSKDKALHEKEIEMLELENDMTKRMSTTTSVDTNMLFSSTHGSPRKSSFLPPGPIRLSATPSIHSVASVQRNSTDDILKTQDIPKRHEPIDSTPKSKGKNILQPQSSTDSSFNFSTSNDGCSQNDFGMAAGLGSKYLFAPDNESTDYASPAKNVEALKNKFFEDDNEDTFELESKQGDDESPIKGPVVGGSSHKNLEGKSHNVERLLEKIDDKQKQMPKFGTPDTPTTIDWNNFSEDSVVDDPVNVALMKLEGTYKNDGSEENGPSSHKSSDSVLVNEVSMLGISDVAGGQPDPKNKRKSLLIETRRQTIMNIPYTPTAAEQGSLGSQESITFQAKDLLAQYEMKDPSLSLENCEQHIPFILMYDSLSIAQQMTLIEKELLSEVDWKDLVNVDLNYSGPPITSWLQLLVQNESMSGINLAIARFNLTVDWIISEIILTADIKLKRNTIQRFIHVADHCKSFQNYNTLMEIVLALNSTAVQTFVEAWRLIEPGDLITWGELKKMPSLDKNYAYIRKLLNNIDPMKGCAPFIVVYLSDLSLNSEKRTWIKDNEIVNYNKFETNVQIVKNFIQRVQWSKFYDFRVDHELLSKCVYLTALSHTEMTQVINRSTNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAP4</accession>
    <name>A0A8J2TAP4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="15">ZYBA0S11-03730g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_03730g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316464">
        <property type="protein sequence ID" value="CDF91477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1029639at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04044">
        <property type="entry name" value="C2A_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04052">
        <property type="entry name" value="C2B_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04040">
        <property type="entry name" value="C2D_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21678">
        <property type="entry name" value="SMP_TCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037761">
        <property type="entry name" value="C2A_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037765">
        <property type="entry name" value="C2B_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037756">
        <property type="entry name" value="C2D_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056910">
        <property type="entry name" value="TCB1-3_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017147">
        <property type="entry name" value="Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052455">
        <property type="entry name" value="Tricalbin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980:SF1">
        <property type="entry name" value="TRICALBIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24920">
        <property type="entry name" value="C2_TCB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037232">
        <property type="entry name" value="Tricalbin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
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            <begin position="319"/>
            <end position="526"/>
        </location>
    </feature>
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        <location>
            <begin position="517"/>
            <end position="643"/>
        </location>
    </feature>
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        <location>
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            <end position="845"/>
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    </feature>
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            <begin position="1197"/>
            <end position="1314"/>
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            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="635"/>
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        </location>
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            <begin position="1472"/>
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            <begin position="9"/>
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            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
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            <begin position="70"/>
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            <begin position="117"/>
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            <begin position="137"/>
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        </location>
    </feature>
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            <begin position="685"/>
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    </feature>
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        <location>
            <begin position="696"/>
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    </feature>
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            <begin position="722"/>
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    </feature>
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        <location>
            <begin position="1386"/>
            <end position="1410"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF91477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1626" mass="180530" checksum="5C627FCD76B63EE3" modified="2022-05-25" version="1">MEDSEAAKAKKGHKFLPRRPHKLMQSKKETRVGLGQKAMQDYGREVLDDANGSPTSKQNATAQSIKSPRKSGSNFSSFRRSTSSKSASKVQSQFRVRGANAPDTDASGNEARPQDMPSVSSQQSRRNVSPMNEINPRLSTSSRNGSKAHSQTHSNGGNTNNADIKGNGSYTPNGSGPNSHLTKNTVTYQGDEPARKMTTHPKPADSSVVAGNYLEELYPWKCIGNFNHKGRGMPDSSDARLIKAYILENFLNDWYYNVAVIVGTCVVSWAFAYCGFSWWSLMFILICTGAVLSTEYRRFNRNVRDDLKRVTVQETLSQRTESTVWLNSFMSKFWVIYMPVLSQQVKDIVNPSLAGVAPGFGIDALSLAEFTLGSKAPAIQSISTNTKTDKGAAEMVFTFAFTPNDVSDMTPVEAKEKINPRIVLAISLGKSFVSKKMNVIMEDINVTGKMRTKIIFGDTFPNIRIVSIQMVEAPIIEFALKPLGGDTLGLDVMSFLPGLKSFVQNQINANVGPMLYAPNHFDVNIEDLMATQANDAAGVLAITIANGDGLKGSDFITNTVDPYITFELEKGLPDVNNDLRTTVKSNTTSPRWNETKYILLSTLNQKMRLKCFDFNDVRKDTYIGEIEVDLADLLGTPSQDGLSQDLKAGTKPKGRLNYSMHWFPAKKSEAVTGRKEIEEEEASEQEGSTQTQTEADSAKDEKKDIDDSKKHSEEHGEKENGKEDDEKDEDEESNDEEEEETDAGIAKLTLQNIKFLNISAAASGTLSPSVTLSLDDVVVKNFRTLRHINEPAWGETIEVFVPSREESELKLEVFDHGLREKKLICEYSSSLEEMFQTLQDGPSFVKGTPQGEIYISADWKPVSMSGLFASGGMRDPIGALRVHVRDINVVDDLSGIGDIDPYFTLSVNRHVDYKSISYSETEHAYFEKVEYLTLLSDKALVNLAVYDYQTVGNDRYVGSAQIPLVEVLKKDSKTGKYQFVDDSKKTIKLNLQQKSGKMSSSYVNISLSFVPCVAVYTPDEYQTVLEKETQLKQRKKEFWDEQATLKDEMKNKPENYEIVKEPDPFEEEEKKLHKKVGLSLEQLLRHNAGVLNLQFLGGTVNHHDCFLQVCVDDIPWPKYTSSKIYGDSLPSDSVNVFIRDLRNSKLLFRFSDKRVPKEEHSIISEFSCNTYQLLKTSYNDPSKVNICGSILKLQCLYTPTSQRIPVSDTVLDTGVLKLKVVSAANLMSADRNGYSDPFFTIDVDGRQHYHSQVVKKTQSPTWNETAEVPVPSRTRKKVKVMLYDWDRAGDNDDLGFVELDLFAMIPKKMYDWELPVSTQGSVKLQACFFPQYNKPLINLHEGKKKSKLGHIATAPSGLAHTGFGLAHGGARLLKKPFMHERSKRKSIGDVDRPASNRKSMDRKSFARDEATDGGAPDISPQLAQARASLDVDRSVPNQDYALVQKLDPESQLPVGSDDLSARIGEKVPSVLSGPVDRKSFARSRSSSNRSLAPGTNYQGTVTLVSSERLAKHVQLKVSLAVGGKIRHLYKSYSQKEDESGVATFDESFNFQGPPEATLVIGAISHHMLTKDKDLGIAQIALSDPLVQQGGNISVELGRGFVVFRLTYGKPTGSSVPPVPPIPQEYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAS7</accession>
    <name>A0A8J2TAS7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ZYBA0S08-01134g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_01134g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316461">
        <property type="protein sequence ID" value="CDF90709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2TAS7"/>
    <dbReference type="OrthoDB" id="431825at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039754">
        <property type="entry name" value="Esf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012580">
        <property type="entry name" value="NUC153"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056750">
        <property type="entry name" value="RRM_ESF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202:SF0">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08159">
        <property type="entry name" value="NUC153"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM_ESF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ESF1 RRM" evidence="7">
        <location>
            <begin position="170"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="NUC153" evidence="6">
        <location>
            <begin position="556"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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            <end position="551"/>
        </location>
    </feature>
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            <begin position="589"/>
            <end position="633"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
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        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
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        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
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            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="229"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="600"/>
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        </location>
    </feature>
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        <location>
            <begin position="620"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF90709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="633" mass="73174" checksum="92B605A23F8EC96A" modified="2022-05-25" version="1">MSGDSDSQKSTDPRFAGIQDDPKFRNIKRKGLKIKLDDRFSKKDLEFRRKAKVDKYGRRIAGESKDLKDFDKYFEKEEKVDDDKDDDENENQERKGKKERSDSSSSSSSSYSEQKDSKALDRARGDVAISSEEFTSSESEVSDESEEESEEEALEATGQDKPEAGEPSRTLAVVNLDWDHVRASDLMITFSSFLPSGGKIEKICIYASEFGKERMQREQVEGPPRELFRKKTKASKYDEDSDSDVNIHDLYEEGDVDKEVDSKALRRYQLERLRYFYAIVYCNSVGAAKDIYSNCDGTEYESTANIFDLRYVPDGMSFDDEPHDECNDLPKNYKPMQFSTDALQHSNVKLTWDETPVDRIEVSKRAFTQKELDDMDFKAYLASDSDESEEANNEEVRSKLKSLVSMPFDDAKTAGKGEEEPDMEITFTPGLDDSQANANEHQEETTIEKVRRKEQERRKARKQKLKELKQKSEQEKKEQLKSVKGKKHDKGMDAKSRAELELLMMDDEVGNEKSLNNKAHFNMNEILKSEKEKTKRDKYQNKDKIVEDNFRPNLSDSRFDEVFENQEFAIDPSQPEYKGTKAMKEILQERSKRAHKKRRPSFDSRVRDKDGEGNISNIVKKLKRKGGNRKSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WUQ4</accession>
    <name>A0A8J2WUQ4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Actin cytoskeleton-regulatory complex protein SLA1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Actin cytoskeleton-regulatory complex protein sla1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">BN860_08592g</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Component of the PAN1 actin cytoskeleton-regulatory complex required for the internalization of endosomes during actin-coupled endocytosis. The complex links the site of endocytosis to the cell membrane-associated actin cytoskeleton. Mediates uptake of external molecules and vacuolar degradation of plasma membrane proteins. Plays a role in the proper organization of the cell membrane-associated actin cytoskeleton and promotes its destabilization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Component of the PAN1 actin cytoskeleton-regulatory complex. Interacts with ABP1, KRE6, LAS17, LSB5, RSP5, RVS167, VPS1 and YSC84.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SLA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="26539at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09532">
        <property type="entry name" value="SAM_SLA1_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11773">
        <property type="entry name" value="SH3_Sla1p_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11775">
        <property type="entry name" value="SH3_Sla1p_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000094">
        <property type="entry name" value="Actin cytoskeleton-regulatory complex protein SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000256">
        <property type="entry name" value="Actin cytoskeleton-regulatory complex protein SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000300">
        <property type="entry name" value="Actin cytoskeleton-regulatory complex protein SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.700">
        <property type="entry name" value="SLA1 homology domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056996">
        <property type="entry name" value="PH_SLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007131">
        <property type="entry name" value="SHD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035800">
        <property type="entry name" value="Sla1_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035821">
        <property type="entry name" value="Sla1_SH3_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF19">
        <property type="entry name" value="ACTIN CYTOSKELETON-REGULATORY COMPLEX PROTEIN SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24081">
        <property type="entry name" value="PH_SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03983">
        <property type="entry name" value="SHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 20" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="SH3" evidence="22">
        <location>
            <begin position="3"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="22">
        <location>
            <begin position="71"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="22">
        <location>
            <begin position="420"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="181"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="502"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="628"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="689"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="839"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1322"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1410"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="217"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="290"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="547"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="852"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="883"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1322"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="1351"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1375"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="1424"/>
            <end position="1442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00064470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00070651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CDF87534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1451" mass="154938" checksum="EF95424FD9CA883A" modified="2022-05-25" version="1">MTVFIGVVKALYDYEPQTDEELAIQENDLLYLLQKSDVDDWWTVKKRVIGLDAEEPTGLVPSNYVEPAAAIRAVKAIYDYEEAQNPDEEITFHENELFDVFDDQDPDWLLVQSKSSGSYGFIPGNYVEPASGAAPAVEAASAQPVPIAPPAAPSVAPTSAAPVAAVPAPAPIAPPVMSPAGVTAFPPPPQHPTRAASASPKVVDDSPKLPARSGGSSREDPPLPARPRDDQSPPLPSRNDGDFGDIPPAKPARPTSTGGLGLAPPGVDSGMKRESFRSGSSYREGDDDFERDRSNGRGVDAHDASDHNDSYENRGLKTDYHTWSISEVDGRKKHKCKLTVGNNKVYFEPSTGPHEEWTIDKLTSYDSEKKHIFLDFVDPYKSLELHAGSNDTCAEIMTVFGEFKGAQRDPAFREIEAASKPKRQGKVLYDFAAESKDELTVKEGQLVYIVNDKKSKDWWMCELVSDGKRGVVPSQFIEPVKEKGSSSGGGFLGSLRKITGNTEKYNARQASRKGRSGGDSSNWRDDEAQSLSEASADKRNAGRRRSTSSSARRPSTTASKSEFPDAKKCRIWADRSGSFKVEAQFIGCTEGKIHLHKANGVKIAVAAEKLSDEDLIYVERATGFSLDKFKSKESGGGDSRNERDRSERERRRRGTKEQDDKEHDRWLREKELEELRKARELLDEERARLREAEQAPPPKPPRPQSTGSAKNVSLNYQPKYDWFEFFLNCGVDVSNCQRYTINFEREQLTEDMMKDINEPMLRTLGLREGDIVRVMKYLNNKFNREPQVAQGQSTGGMFSEADGSLKVANSGAPIAPQLTQNLLPQATGLQVSNNASAAVSSAPAPMGAPIQSAPTVSPAPAGPVSAAPTAGSSGQGAEDDAWAAKPAAKAEANSSNNNAEFTGSMQDLIDLRPLEPKKKEYVSAPEPKLKDLEPVKTGTDNTTAALPATLTGGTTLQPINPFKTGGNNLLPITTGFVMMPIATGGIMPMQRTGGIVMPQTTFGAQATGGIMQPQRTNSGLVSMGLQATGSIMQPQNTNAGLLPMGTGTALPPQNTILPSQSTGIMLPVGTGSVLQPQRTNSIFPTTTNAGMMPQTTFGAQPIGSTMPMQATGSALPRLGSVLPVQKTGSGLMPVQGTGSALPMQTTGGILPMQRTGGLVPLQRTGGLPMQVTGNLVPLQRTGGLPMQVTGNPVPLQRTGMMPQTSFGANITGGANMMPQTSFTRNITGGANIISQTTFNGPAMTAAPNMMPQTSFGGPNMTGGANMLPQNSFGGFNVTGGPNIMPQTSFGGLNPNVGGNMVSQNSFGGGQMTGGANMMPQTSFGGPQMTGGFQQNSYNNQGAPAQMQQTNGFSSMPQQGQMSGSFQQSNPAGPPNQFTGGPMQQSFPNTSFNSAVDNATQGMQDMSFSQAPLQSQPTGFGFGNGPQQQSQQQQQPKQANLFNATADNPFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WVI9</accession>
    <name>A0A8J2WVI9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">BN860_17018g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_17018g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WVI9"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF87900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="321" mass="36455" checksum="58F3CB4EB1C1D71C" modified="2022-05-25" version="1">MDYISDIASNILDPTIETWCSQKKSQAEYFSEGTATNYWFIAPYEETIQNKKKNALGDNYASLKVASIPTGEKLEVLFKRGDNSKLINSSELDRQERFSHAAARIMTNAGNSWRMPKLTLSNRGLLDSSLVSAREFWKEQELLLSKGFDDFIIAAFKDFWKNQNCASWSEIDDPILDAALEFWESYAATNNLPAILTESHEKFSSCDYAVIDVELGDEDLSSQAVSINVTVKLAESDSEIETEVEEEHSTTVEEHRKAPTNVQQERDKLAWKNIGLMDENWTQNEKIVRESTQLEVTDDGVDHNANLGWIYEKLKKATKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WVK9</accession>
    <name>A0A8J2WVK9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">BN860_17722g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_17722g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WVK9"/>
    <dbReference type="OrthoDB" id="2125396at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF87931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="399" mass="45579" checksum="ED614B7726AB2D28" modified="2022-05-25" version="1">MANKLRPKKAKAPYRKYVAGQGFVHTRGFTSQEHDLKEEESVPKSSYLDIQGPLALPWEVQALIIQMIDEIGLQHLLVCKTWYYICIPLLYEFPQLTSKNYAAFVSAVVDNKKKKLGEKVLVLDLSMILQSGKNSSISKLLRRCSPSLERFTAPQTSFGYAPLISLKACHELKYLDLGLLSETVKLKELFLAIQNFTELTHLAFPRSSVDCEGFREFKWPPNLQYLKLSGGITNEFLAETRWPRSITTLEFSSCPRVDEHGLYRVLAQIGDNLRQLYFHYPLPQLHDNSLDYVFRYCSNLISLQLMVDYCSKWAFSEHMLPPLVHPRPLRTVYLESSGSLGLAFKIHPDDFTIAIMESRLPCLKSISVSSKLNWDVKSEDVTDLVSVFEDQGGSIYLSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WVN5</accession>
    <name>A0A8J2WVN5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_18426g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_18426g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WVN5"/>
    <dbReference type="OrthoDB" id="4084551at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028000">
        <property type="entry name" value="Pma1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14610">
        <property type="entry name" value="Psg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035269560" evidence="3">
        <location>
            <begin position="23"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF87961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="395" mass="45797" checksum="E1E4CC4DA76E123F" modified="2022-05-25" version="1">MSSIYKIWAVVLLLAQGNDVLGYRPVVQPKQQLTTSEEPSPWYRTIYSDKVELVTPTVIAGVTFSRKPLETADPLEPWVSLDKQGSPKTINPEIKNGRTKKAHPDYSTYFKNVYTKTLGYEDLKAHNMDYGDTHEELTFEDEDDTYTSLNPLIRCTPQRYFNKGLTQNVPSDPFCTPHEDSMWHVGKTYFLTWYTHFFKDEHSNEVADRVRVHMAYVKEKANEKGMLKREPPSATFFKSEWIKNVDGVFPVEVLHEWLQGVRTRKVVISVQPEYVPDEEFDPLLYGVFVFLDEGSKVFKHTKEELALRDAGITDDKWYYVAISIPTMVILSLVLMYFFINANRSQRDFSDITNKTLKKKHRVLGKVADMKKFKSMKNRPYSELPTYNKGSSGKQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WWU5</accession>
    <name>A0A8J2WWU5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP-dependent RNA helicase DRS1</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ATP-dependent RNA helicase drs1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">BN860_08526g</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-binding RNA helicase involved in ribosome assembly.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WWU5"/>
    <dbReference type="OrthoDB" id="10259843at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17947">
        <property type="entry name" value="DEADc_DDX27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000842">
        <property type="entry name" value="ATP-dependent RNA helicase DRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 17" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="22">
        <location>
            <begin position="238"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="20">
        <location>
            <begin position="269"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="21">
        <location>
            <begin position="455"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="22"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="119"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="671"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="626"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="238"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="133"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="173"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="205"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="728"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="738"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00043881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CDF88364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="754" mass="84554" checksum="C3C7C82BD39C69B0" modified="2022-05-25" version="1">MGRRVVDKGKLTLDFVPTISDSEDAVPILDSSDGEEDSERSLPTGQKKYKKNAQSADRRDKEGASKDLDPEFKFEAGGNDVVSNLQSWDFVGDEPNEDDIKKDVDLDGIIRRKGGLIKTLRVESGTTQTQDNMSKEKNKAVQEKESDQTELVGDDDELALDGFGMGAAQMSPEIDESGSDEELGQKEESEQEEESEQEEQKEQEQVSDEDEEQDSPEEMAKFYAPQSESEEAKKQVFKNFNSLSLSRPVLKSLSDLGYVTPSPIQSATIPIALLGKDIIAGAVTGSGKTAAYMIPIIERLLYKPAKVASTRVTVLAPTRELAIQVADVGKKLSRYVSGLTFGLAVGGLNLRQQEQILKTRPDIVIATPGRFIDHIRNSSSFNVDSVEILVIDEADRMLEEGFQDELNEIMQLMPSKRQTLLFSATMNSKIKQLVSLSLRRPVRVMIDPPKQAASNLQQEFVRIRKRDHLKPAVLFHLLKKLDGAAEKRIVVFVSKKEMVHRLRIIMGLLGVGVAELHGSLTQEQRLQSINKFKSLEVPVLICTDLASRGLDIPKIEVVINYDMPKNYEIYLHRVGRTARAGREGRSVSFVGESSQDRSIVKSAIRGAEESGEGNLPVGRNVDWKEIEAVNGTIESLSDTVKEILEEEKAEKEILQAEMQLRKGENLIKHKQEIASRPKRTWFQSEAEKKNAKVADSMSNNKKSINSKKRKRQEALEDKARPYKKTQKNRSDDQERSFKKQNNKKMKDKKKRGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WWX3</accession>
    <name>A0A8J2WWX3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BN860_09208g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_09208g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WWX3"/>
    <dbReference type="OrthoDB" id="2399539at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14723">
        <property type="entry name" value="ZIP_Ppr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052202">
        <property type="entry name" value="Yeast_MetPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782:SF1">
        <property type="entry name" value="PYRIMIDINE PATHWAY REGULATORY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="31"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="157"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF88394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="851" mass="97163" checksum="FCA01092EC9D732B" modified="2022-05-25" version="1">MKRASKEMERDNVVRKRPSSVVVGITKSISACKRCRTKKIKCDHEFPSCRKCARANKPCVSLDPATGRDVPRSYVIFLEDRLTAMMNRLRERGEDPEKVQGNIPMTSEDNPCNIELYEERLRNDHQVPYDNLLAGYLINKGTSMQKGVGVADKDTTQNENSLEQSNNTRSAELDESNKSLTTLGSMKNNASNSYLGDSSGIPFAKLVFTAVNFRPDFIDEESDDEIRQREAKYIDYANAETTSVFEPLWLPPKEIAERIISRYFVDSNSQLPVLHREYFLKRYFEPIYGDWDTSISLASDHTKINTDFKIPRGCTYISGEEPWYEVWKRRHDQDESGINLPVKYQIPYFFLNIVLAIGESTQVLRSDTVRVVSFKRRAVQFSKALYSSTNRLEALAGTILVAIYSLMRPNVPGVWYTMGSALRLTVDLGLHAEKLNRNYDPFTREIRRRLFWCVYSLDRQICAYFGRPFGIPEENITAKYPSMLDDALITTSNDNIVDYSKVNSSMASPKVIALAMFKVRRIQASIVQVLYAPNGEVPRKFADLESWKSQMHSSLDTWFRKEVPKTYKKMNSKFNTEFFNLNYWYTKSMLYGLSPKILSLDDYAFNVVYDSTRGTIDVFYKLCHNGKINYTWVAVHNLFMAGMTYLYTIYYSDKGIIDGQQVTENYVSKLLHVLKNMIGTCEAAKSCYKICKVLSAAMLKLRFDENGAQSTTTPQYPQVIAAVSSNASPVDSLEGTLEMPKIKHDSFEVPLGQFFDELEKVTMQSDPESTRSRMSNNNSPQLPNALHADIDGDENAHRNKMAFDKDAQRVLDMVSQVTTDSIWNEFFGKIGINDLSSHDDGGEDLEPNNFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WXM3</accession>
    <name>A0A8J2WXM3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">BN860_16314g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_16314g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat PLAP family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WXM3"/>
    <dbReference type="OrthoDB" id="10265988at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010992">
        <property type="term" value="P:ubiquitin recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000175">
        <property type="entry name" value="Phospholipase A-2-activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.870">
        <property type="entry name" value="PFU (PLAA family ubiquitin binding), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015155">
        <property type="entry name" value="PFU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038122">
        <property type="entry name" value="PFU_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013535">
        <property type="entry name" value="PUL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF0">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09070">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08324">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51394">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51396">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="96"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="134"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="216"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="PFU" evidence="9">
        <location>
            <begin position="351"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="PUL" evidence="10">
        <location>
            <begin position="467"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF88697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="719" mass="79522" checksum="9C1F688DD38037CC" modified="2022-05-25" version="1">MYQLSATLKGHGQDVKDVVAVGESQLISVSRDGTVIVWTKSEDSQWQSAIISRRELFVNSVCFREKDSMVFYGAKDGLISGSPVFAKFDEDPVYTLIGHQSNVCSLSCDGENVVSGSWDKTAKVWENGVVKWDLRGHEASVWDAKAVPTMPDHYMTASADKTIKLWQADKCVKTFSGIHNDVIRHLEFLNSGNFASCSNDGTIKIFNMSGVVLQTLEGHDSFVYSIRAFPNGDLVSCGEDRSLRIWSSTGQLKQVIIIPAVSVWCVDILPNGDIVVGSSDSMLRVFTNAAQRTASAAEIEQFQQDLQNSSINSQVMGVDESKISPYEVLQSPGKKEGQIIIVKAPTGLVEAHQYSQGQWLKVGDVVSNSGSDQKKEFQGRMYDYVFDVDIEEGKPPLKLPVNANDNAYAVADQFLARYELPQSYRDQVVNFILQNTTGVSLDPDGAPAVVSTTSETPSVPTISNSYKVLPVKQYLSIKNYNPDSIFMGITKLNAKEQTFNDENLAQIGTALHDLDQNWEVLYLFALSMRSKWSTKTPAYDIVRLVVSRLSSSDDISEFIEEGLGNQDITIAMLTVRIFVNCFDNKKWGLKLMSSKNVYESIFRTIETDYPEARVQQLQNLAIAVATLLVNYSSLIVQDPTGMADAVAPISDALNTKFGNQETFQESEEAAYRLLVAFGNLSIVEPSLKQFAGSISWIKSVKAYYGNIVRFQEVLLDIGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WYC2</accession>
    <name>A0A8J2WYC2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S03-05336g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_05336g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6365676at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018844">
        <property type="entry name" value="Dnt1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043185">
        <property type="entry name" value="Net1/Tof2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28196">
        <property type="entry name" value="NUCLEOLAR PROTEIN NET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28196:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN NET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10407">
        <property type="entry name" value="Cytokin_check_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolar protein Dnt1-like N-terminal" evidence="2">
        <location>
            <begin position="68"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="577"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="931"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="490"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="577"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="615"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="629"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="706"/>
            <end position="722"/>
        </location>
    </feature>
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        <location>
            <begin position="728"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="766"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="781"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="813"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="931"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1264"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1318"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1405"/>
            <end position="1432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF88935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1468" mass="166281" checksum="09E8621E068D8BDE" modified="2022-05-25" version="1">MFKLQVVLVPPSAQNVPLGYGLASSTAESSQLIGINPMTAPRNQETTLPYAPSGNTSLAPYHFAARLRKFLHFTKSANTLLELSAEIDEKCAKMYPYVDELEILSLQDSQGCDLDPDFLVKDVFTMDNVVRVIIKNELEIDDSSPVSSYRAAKRKKLNSGIAQSPPSGHQQSQVLASTASSGILNIAKKRPAAFAMRHSTNNNMRVSTPLAHQIYPLPNSHDRMVSNNSEDEDDDPGERSFLPPPAQPQSPPIRISSGMDTGKKIKSMIEDDTVSRSETVDPDKLRQQRLLSGTPVRSAMTPNRVTLTGQRVVSEHQPPQPTNAPLFTAANGNRKNSFATTRITSGRLSIPEPKIAEVEKELKEGPSSPSSMLPPKPDRIPMKKPLQETEEQPSDDDYSSEDQETDRQVRAFIQRQTSSIADNNGSPTKNSPLSDDDHVHLAELPQTRPRSVSSQQRKTSLEAKVEHKLSGGYPEEEPLRIDNFSEDEDSGAHDKEQDRQWEGEIDRNRIEQKSNAEENDADSNDTANETVVVRGNLQKPAPTNSINKEELLKFMEGDYSSAWNNHSSKGKPYTTVLHKDIDNSKPDPRNILPEKMTRSAAKRAAQLLAGTMEHGSGHEPAKVVEDSSSDSSSGIESDLSQENENRISVAENNATKTLNIHPLKETVIEGNDSTARANALRRLSKSDSERSEGTSFGSSTDQEQDVPSHVEEQVGGHKETLPAHRTRLVSNSLNTSEKNFSLNQPEKPLIHISPAATSPNGAILNKENDQLSAEKELTGSIPKSSSIVRSNNLASRHNTKKADTEIPTSSSSLEKDNPKAFIKEAQKDVKSGETKTRKTGKEKQKQIEKESKQKAREDAKKAKEEEAARKRREREEKKKLKQEEMEKKRKLQQEEKKRLQAEMEKKREEEFKKQQELLERRKQEFYEKKRQEELERQRREEEGRKKEADIVKRRLEELGRKKREEMERKKQEDEEKRRQEELARRRQEEAERKRQEEQERKKQEKIGKKRLQEESEKKKQEEEEKKRLQEESEKKRLQEESEKKKQEEQEKKRLQEEAEKKKQEEQEKKRLQKEAEEKKRIQEEQQKKRLEEEAERNKEEERKRQEEAEKKRLQEEEEKRRKANLEKKRQEEEQEKERQAKLEKKKQDEELQQKRRTDLEKKKQQEELQKKRQAESERKKLQDEVQRRKHADQEKKKQEQVKKKPKALSVAKPAKSPAAVNGSDKLKELKARFTSSKTYVPASSKGSQQGNKGGFNEDTESSDEGSSTEYSSSSSEEEEEPSSKKSRRKVVDTPKGSLTTGRQKPAPKELGGLEAAPQSTQRSDFSSQSSQNQVPVTRMMEMSSPSRSREDTSANSSILAGLPKKIRPSLSSLSDLVSRGVPEVKEKSNSSQNSASVHPKKVDTDSDADESDDNNDSETESDSSDSDSDDSDSGNNSFISAKTANTALGKKKKGNGGFASLLRDSQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WZN1</accession>
    <name>A0A8J2WZN1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_05358g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Mediates the transfer of lipids between membranes at organelle contact sites. May play a role in mitochondrial lipid homeostasis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the VPS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="428159at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026847">
        <property type="entry name" value="VPS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056748">
        <property type="entry name" value="VPS13-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017148">
        <property type="entry name" value="VPS13_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026854">
        <property type="entry name" value="VPS13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009543">
        <property type="entry name" value="VPS13_VAB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166:SF93">
        <property type="entry name" value="INTERMEMBRANE LIPID TRANSFER PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25037">
        <property type="entry name" value="VPS13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12624">
        <property type="entry name" value="VPS13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25036">
        <property type="entry name" value="VPS13_VAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037235">
        <property type="entry name" value="VPS13_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3 4" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Chorein N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 13 VPS13 adaptor binding" evidence="6">
        <location>
            <begin position="1876"/>
            <end position="2449"/>
        </location>
    </feature>
    <feature type="domain" description="Intermembrane lipid transfer protein VPS13-like C-terminal" evidence="7">
        <location>
            <begin position="2979"/>
            <end position="3084"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR037235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF89483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="3110" mass="352175" checksum="F404EE5C102F4884" modified="2022-05-25" version="1">MLESLAATLLNRLLGSYVENFDPNQLNVGIWGGNVKLKNLKLRKDCFDGFHLPIDVKFGILGDLVLNVPWSSLKNKPVKVNIEDCYLLCTPRDFNSLHSQEQVERELRVKIQKLASWEVASQARAAAEDPSANKDQNESFTQSLITKVVDNVQVKIKNIHIRYEDMNAHLSKDACSVGITLNELSAVSSDSNWNPSFISITQDITHKLLTLNSFCLYWNTNTGSLEDEDGSLDPEKMIAKFKDCVATSGVSVPNFQYMLRPVTGSGRLTVNKLGPTESRPHIELQMLYDEFGLEIDDTQYADILHTLSSIHLSQKTQKFKKKRPNFAVEQNPREWFRYVGHCVMEEIHERNEMWTWDSIKKHCEQRRLYVKLWSKKLLLGSWDQPLPDREEEKTLEQLQRELSFEDIILFRSLGRKEYAKQKLKSRDSSPATSQQGNTQGGWFSSWWGGEHSSQNSDLTITDEQRKELYDAIEFEEKDGGLGDMQDMQIPRDRTTMKVTGLLRKGAFSMKKKDHVACLGEVIFENCEVELLQRPDSFLASFKLHQFKVEDGSPNTLYKHIISVRNPDSDIAEKSLTQSNPLFQLTFESNPIDESADSKLNVVLRGMTVFYHVHFITEIINFFSPPKQHMDTIGAIINAAEATVEGWTTQTRMGLESLLEEHKTANVTLDLQAPLIILPLNPHSWETPCAVVDVGHIFLKSDLVPKEKLDELKQMSPEEYEKIDDSELNKLMFDRYQLCLQDTQMLIGPDVRSTVSSLNGAAKGSHFSILNKLRLELTLDLSILPKAFNLPKIRAFGRLPQLKLSLNDDQYKIMMQLLDKCIPSPLSVDADNEFRETPGNFDDAKKKAQAQLSENLKILEKMSPMELAQKLFELHFDVDDIQISMFKCLDSESMQCKKLLDFLGGKVKFNFVRKAKGMNLQLKVHSLEVEDHIEESNNDEFKRLISSGKVENDQVKDLFSLEYSRIQRIVTHDNTLIEVFDQDVSMNMAQLQLVLSPKSLLTLLNYALTTFTNPDAPAAPADALRHNEENREDAPQKFNLNISVAGVVMVFNDDSLKLATLVVSAGDFSLLYMPEKMKVNMSLGGLELTDETTEDLPRDSVFRKLICMNDHELAEISYETFDATDNPENYDSFLKYNTGSMHINFVEHSINKLLVFFCKFQKMKTLFDKAREVAYNQAPSIEAVNKMKMDVQIKAPVIQFPRIVATQDEIYDSVTFHLGEIFVENKFTKNEADVEINHIKLGIREAQVSSLLNLKEGAKQQLHMVDNLCLTFNIDHNSHADSTSPKFLIKGYFDPLMSSLTELQLNYLYEISERMSSAFDVSDASAVELSEDVIYANAFMDSPGVESTPEGITVTNQDEEDKINPEGLKMDFSFEAPEISLTLYHDTAATQNLYKNGLTKLTFQDIAFSAKYNEDGTGEGNSHIATFTVEDIRDMKDNKHTELIPKVVNDNYQFMASVTINKLESGKLINVSNTIDSPQLILAMDYLFALKSFFDAAFDKNKADITVEDENNAKEQNPSLPAAEIAPSGTKLQYSFNIVNAAIILLADPSDIHSEAVIFNAGQLLLSDQNVISLRANNVGMSLSRMNTEDKSQVRLLEEFSSSLVIDRRESTPEKLLTNVQISVEPMILGISLRDIRLAMLVFNRMVSQMNGKSEEVQSEENSSSLAPGGFSKEFERKLSKYAPSFVSSIEAVSGHASVSHEVVMRGENLEADFGGVRLFLIGDVHEMPILDMNVKPFQASVKDWSTEIDALAALEVYVNVFNYSRSSWEPLVETFPMSFHLSKSSANKDAKVMFDVISKKTAEVTLSSRTIAMLSQIPGTLTAEYRLEPRGAAKPYKIHNDTELNMDIWIVTDNVQERKSLVTLEAGSTLPWEFEDWRSVRENLEADNSGLILGAAISGSRYQTTMKVDATYEGELLFVLQPAVNHVHSRITCELELGDDNIKTITFRSTLLLENATSTDLQIMVEPEQRSQSFSFELKALSSRSIPVEYAFSSSLRIKPLGDVEYDWSQQPIMWRSLLKRPMSLQCNSLAGRNENFHIEVNGKYDEKEPLAQIFPHMKLVLSSPIIIENLLPCDLKFSLFDKKEEEKNMSILKKGERIPIHDVSLDNFLLLRVQPLVDDASMSKPSIVNTAISSVLKPELSLVLKLAGGQKFNAMLHYQSVEGTRAKVIRIASPYIIFNETDRDLYVQGDPSNVCQSKVQIEDDKRFTTPKMFSFDVNSYKKNRARIRFKESDWSIPLSFDALGQSFDTDVSIPNKEQESNFGVTVSEGEGIFLLSKIISIAPRYILHNFLDVPIEICEHGSTNSLDVEPGKSLPLYKMRNIVHKQLMVKFLGSSSEWSSPFFIKDIGFTYLKVLQQDSHLLLKIEIILENATVFIRIKDAENRWPYSIRNFSDQEFIFYQRDPRVVDDYYDYDEFDELDEAEYKPYYYRVPPKSVMPYAWDYPAARQKKLILTARGRRREIQLAEIGNLKPMRLPARTSNEETVSMDLNVIADGPTQALVITNYNADVSLYKLRNKNTSSSISVSSSTGEGFEADAEDKNVYAKIVVSFKGLGISLINASLEEILYLHANGLEMRYNESDLYQTFSWKLKWLQIDNQLFGSPFQNILYPFAVPHTSHEIENHPVISGSISKVKDDSHGLVYFKHATLLLQELTIQLDEDLLIAMLDFVKLPGAVWNVPSKNTHYFDEVTLPQCEDLKFSDDMYFEIFHIQPIMLHVSFVSSDQHQLLKEQQEKLGVEYQGTMLVFMDMLTMTLANVNDAPIKLNSLYMANLRVPISTLYKAIETHYGQQFFYQIHKILGSADFLGNPVGLFNTISSGVWDIFYEPYQGYMMNDRPQELGLHLAKGGLSFAKKTVFGLSDSMAKFTGSMAKGLSITQDLEFQERRRMQQRMNANKGNLLSSTAQTFANTIGSGFTGMAFDPMTGAQKEGASGFFKGLGRGLIGLPTKTAIGILDLTSNLSQGVRSTTTALDVPAAARVRLPRYVDYHEKIIKPYDLKEAQGQYWLKSIDGGLYMNDNYLAHVVLPGNELAVLVSMEHIIELNIGKLESMWTTDYYGIQGIVLQRGGIHIKLKTQSEYFIPIANPQERKSLYKHIAIAVTEYNKYCEAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X2P7</accession>
    <name>A0A8J2X2P7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA polymerase gamma</fullName>
            <ecNumber evidence="4">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Mitochondrial DNA polymerase catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN860_04654g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Involved in the replication of mitochondrial DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA polymerase type-A family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="4"/>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X2P7"/>
    <dbReference type="OrthoDB" id="5588663at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005760">
        <property type="term" value="C:gamma DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000035">
        <property type="entry name" value="DNA polymerase gamma, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.390:FF:000003">
        <property type="entry name" value="DNA polymerase gamma, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019760">
        <property type="entry name" value="DNA-dir_DNA_pol_A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002297">
        <property type="entry name" value="DNA-dir_DNA_pol_A_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041336">
        <property type="entry name" value="DNApol_Exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10267">
        <property type="entry name" value="DNA POLYMERASE SUBUNIT GAMMA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10267:SF0">
        <property type="entry name" value="DNA POLYMERASE SUBUNIT GAMMA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18136">
        <property type="entry name" value="DNApol_Exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00867">
        <property type="entry name" value="DNAPOLG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00447">
        <property type="entry name" value="DNA_POLYMERASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family A palm" evidence="17">
        <location>
            <begin position="691"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1185"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1192"/>
            <end position="1205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CDF90593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1230" mass="140842" checksum="7D5D0BA58565B099" modified="2022-05-25" version="1">MYSRLRGKYIVYSQAVPRNVLHCRLCRTYSASSSAEPEEPTINPVGIQYLSRNLHKQLFTDKIPKHDKDPMTPKEREELVDLSRHFLKNNNLLGKKTSVSTPISLELPKLQGASLDEHFQKLGHFASEPYKSMCDAKFTDIVSRPKKWVRKKGWMRYEPGKEPESVPWPIEDTIVFDVETLYKISPYPTLAVALSSGAWYLWCSPYICNSNSFEHLIPLNTQERTGLVIGHNVGYDRARVLEEYNLRPSKTFFLDTQSLHVASSGLCSRQRPKFLKNRSLGRHPASEDEETIDDGRNVTTLLETASPETEEDPWLKVSAMNSLKDVALLHCGIDMDKDPRDRFATTDKAEVIDDFQSLVEYCATDVDTTSKVFDKVWPLFLEKCPHPVSFGALRSLSSCILPTKYSEWNNYLQRSEAIYQKSKQSIENKIVQIVEETVKLKDSKSIEEINRDPWLRQLDWTIKPMKMTKKGIPVKNQKLPGFPEWYRSLFPNKKETRPQITIKSRNIPLLFKLSWENHHVVWTSCGWCFAVPADLTEKFRAKNYVLADQKIIEDYRSVTNAQLPRAADDHVLFKVPHPNGPQFDCTVLLSKPYIHYFEKGILTSESEFAREALHMNSSGSYWMATRERIFNQFNVPSVKFPGQFRALDGVTRTKEDLGIILPPVIPMGTVTRRAVENTWLTASNAKKNRIGSELKTQVRAPEGYSFVGADVDSEELWIASLVSDSVFNIHGGTAIGWMCLEGTKHEGTDLHTKTAHILGCDRNEAKIFNYGRIYGAGVKFAAQLLKRFNPSLSDEEAKNIANRLYENTKGKTKRSKIFKKFWYGGSESILFNKLESIAEQDEPRTPVLGCGITFSLMKRNLRSNTFLPSRINWTIQSSGVDYLHLLCCSMNYLIAKYNINARLCISIHDEIRFLVADKDKYRAAMALQVSNIWTRAVFCEQLGIQDLPQNCAFFSAVDFDKLLRKEVDMDCITPSNPTPIAHGKSIDMSTLMEMPEAFLTSPDSRVDVSHYPATYREPVFSRYNRAHNKQYRKYLLKMQLQNCKWKVDDLEKTYLQENERFSIIQKDGEPYGLLDYLRDIRENQKNKRSIMDTTMFGNKEVSFNMNEKHATTSSRDIQAENVPPNACRRKRTNVASKRRVPYGGNPGFDAFYSSIDLSAKKGMIKSIEIPADLVVKDLLADAAEDTRGTNTSKRKCPIKKPKKKPHEQLDKKPRIAIGDRNGLLMQQKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5K7</accession>
    <name>A0A8J2X5K7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-dehydropantoate 2-reductase</fullName>
            <ecNumber evidence="4">1.1.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Ketopantoate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_17876g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16233"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.169"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.169" evidence="4"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5K7"/>
    <dbReference type="OrthoDB" id="73846at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
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        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="F:NADP binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="2-dehydropantoate 2-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
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    <dbReference type="InterPro" id="IPR050838">
        <property type="entry name" value="Ketopantoate_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43765:SF2">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43765">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="10">
        <location>
            <begin position="9"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="11">
        <location>
            <begin position="219"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF87938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="371" mass="42150" checksum="8D943B7277E3F073" modified="2022-05-25" version="1">MTLDAKPLVHVIGLGSMGAILAVDLLRFADCSVVPMFRSKKRLEQFQKDSQSTVGIRKLYLKNSPLIQKKFTLSACPESFTPCAIKNLVVTTKTYQTKESLVPYLPFIDQNTNLILIQNGLGVVEVLKEEVFTDVSKRPQLFQGVISHGCFQDQGFVFNHAGAADLKVSRLPWDEGDMVQTLDDANEDMKNNELIKLFTSKPFAQEFNVKFMTYQELLLGQLYKFLVNACMNSVTAILDCVNGEMVGHCSRVFELIVDESLQVLRLAYKPLFDYEENYSGKDTYPQLKVNSVLNTMDMVKNIENIGCVINGKNSSSMRQDTLYLRDTEIEYINGYIVSLGQRFQVDTKVNKTIQEFVNLRLHLNRRRASMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5T4</accession>
    <name>A0A8J2X5T4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_18998g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_18998g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="5552842at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000402">
        <property type="term" value="F:crossed form four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070336">
        <property type="term" value="F:flap-structured DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16963">
        <property type="entry name" value="CCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039197">
        <property type="entry name" value="Mrs1/Cce1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015242">
        <property type="entry name" value="Ydc2_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28072">
        <property type="entry name" value="CRUCIFORM CUTTING ENDONUCLEASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28072:SF1">
        <property type="entry name" value="CRUCIFORM CUTTING ENDONUCLEASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09159">
        <property type="entry name" value="Ydc2-catalyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mitochondrial resolvase Ydc2 catalytic" evidence="1">
        <location>
            <begin position="71"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="384" mass="43733" checksum="A4E6773949D3BDC3" modified="2022-05-25" version="1">MPKTPKTRSAIEALDLYCRKANGRTLKTLGVCIGANLGHNKTEARERIVHQAKYLEILSSCKSNNGVTTLTAIDTGVSNFAYSTFKWYKKDELPTLVDWNKIDLRQKFLPSGDGKMSIDPRDTFLVGHKLTEMFTAILPIPDMFTIERQRIRSQSSKTVLETVFMSNIMEQILYSNLKNKQIYYETNLKYVVNSSSPQRMVQYWCALRSLKSLLQEYKDSNSTSGGAPPIESGNQCNKTTKVIRINLVRKLLRGSLGLDDYKKCLLTPDWKTKIARQVQAKKKFKLFDCIETGPATGTRKDDDLADSFLHGLAWMQWLKTYEEITEITSANGGSFHQSVLSSFNKYCMELKTEWLNMSHATRSELAELQLNEASQEKMKRAILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X697</accession>
    <name>A0A8J2X697_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">BN860_13674g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_13674g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X697"/>
    <dbReference type="OrthoDB" id="66510at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043291">
        <property type="term" value="C:RAVE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028241">
        <property type="entry name" value="RAVE2/Rogdi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13618">
        <property type="entry name" value="LEUCINE ZIPPER CONTAINING TRANSCRIPTION FACTOR LZF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13618:SF1">
        <property type="entry name" value="PROTEIN ROGDI HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10259">
        <property type="entry name" value="Rogdi_lz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF87753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="339" mass="38106" checksum="C3DCACB362503A7F" modified="2022-05-25" version="1">MTAEVYPNDYFGGPDKLDSAQNNANECKWLIEEIIKPELPNIIDNVERCLDMLQSEQVFRIPISSGCNETSDRPSVKGIVARQAGQLVDFQAVVRFPEFHNGKPILFKKSPESHFPLQQIETITGHLQTVLITLEDLQITMEPKSFVDKIGQVLELLIKSINLLQNPPRDFSFPDSGNAAMKLLFDDHEALSQTAHHEISLEIVLFKKELCVDFRNLIKVTKKPWCEIDPGTGKSFGDKIKDQLTTDRSKNLSSVLKENGVQIEQATFLNNLMSNFSAEITTLPQAQNYLNRCVTFNDKVVIEIAKVALTTSDPFLISTSTKLSSLENSVSNYFTNLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6G9</accession>
    <name>A0A8J2X6G9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BN860_12596g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_12596g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6G9"/>
    <dbReference type="OrthoDB" id="1937934at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00105">
        <property type="entry name" value="KH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22438">
        <property type="entry name" value="KH-I_PCBP_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="81"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="162"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="345"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF88538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="425" mass="46912" checksum="FD3C24AE94020CD7" modified="2022-05-25" version="1">MMANVEENGVSSLNILKRKKEDCAEEQLEAEIKRVALHDDAECPTTSSVDSIELDGRDAGRFSNGESLENLSEELRDSRSRQTHLRMLCLVKQASMVVGHKGETISRIKALTSTRINVSDNVRGVPERVVYVRGSCENVAKAFGMIVRSISDKSENESLEDIPTTINLLISHHLMGCVIGKHGSRLKEIEELSAARLSAMPQQLMMSNDRILTVEGVADAIHIATFYIGQTIMNCKDVFKSKKAIFYQPSPVYSVLLNGVGPLAYSHQQHHQYHPADKYRNGKRLPRSSVMFMMPAAAQTVQQRTPVMYTAANAANAPAFTPNFMIPNVRIIDGPVATPVAQTMAIVQQEIYIDENFVGNIIGKDGKHINSIKESTGCSIYIDDPIGGALERKLTVRGTSMGSQAAIMLISNKIEIDKVNKERKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6J3</accession>
    <name>A0A8J2X6J3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="7">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_13278g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="7"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6J3"/>
    <dbReference type="OrthoDB" id="407390at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF25">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING)] KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Branched-chain alpha-ketoacid dehydrogenase kinase/Pyruvate dehydrogenase kinase N-terminal" evidence="8">
        <location>
            <begin position="80"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="484" mass="56348" checksum="2626BE0653BE5296" modified="2022-05-25" version="1">MRQLSCCCRQLRNPVASFDISRIPNFTKYSAVKPINEDLAPHVCETMKAFPSRSKHVNQQHYYHNWDVLIKSYAQRKPHPMSLTQLAQYYDDSTKLTKQKIINSGKFVKEELAIRMAQKLKLLQQLPFCVVNNFHFVQVYESYYNIFERFRKFPQIRTLEDNSKFAEFSTSILRDFNTLNLPHLVMGALECTVLDLYPQDKMDELLSDLMRARISRRLIVEEHVSITQNYLSGKKENKLVLGDIFQQCSAKDYIIDASQLCQKFIRDMYFDNIALPELIIDGEVNLKFYFLPIHLRYLLYEILRNAYEATMKEYIRRGLQKPEAITVTVIENSQSFLFRISDRAGGFPYNDRNIWSFGKSKEKARESLANFHKLPGLQTVSIYDQLESDLAVPSVNKPYLNTSLDPIDHSSLTGQEFKFEKPLMKLLERSHRYKLGVGLAMCKVYADYWNGDLALHSIQGYGTDVVLKLGNLMNNVSKLQLDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X722</accession>
    <name>A0A8J2X722_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">P-type Na(+) transporter</fullName>
            <ecNumber evidence="19">7.2.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">BN860_05754g</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>K(+)(in) + ATP + H2O = K(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75815"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Na(+)(in) + ATP + H2O = Na(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:14634"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IID subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.3" evidence="19"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X722"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008556">
        <property type="term" value="F:P-type potassium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008554">
        <property type="term" value="F:P-type sodium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02086">
        <property type="entry name" value="P-type_ATPase_Na_ENA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000040">
        <property type="entry name" value="Na(+)-exporting P-type ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000015">
        <property type="entry name" value="Sodium ion P-type ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000039">
        <property type="entry name" value="Sodium P-type ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000047">
        <property type="entry name" value="Sodium P-type ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006414">
        <property type="entry name" value="P-type_ATPase_IID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01523">
        <property type="entry name" value="ATPase-IID_K-Na"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="17" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="281"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="310"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="774"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="811"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="848"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="903"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="950"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="983"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="23">
        <location>
            <begin position="11"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00035017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00035029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="CDF88254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1050" mass="115990" checksum="D75F178A334268BA" modified="2022-05-25" version="1">MSSTESEEDYLFHTLSVSATAELLSTDCDQGLSTAEAEERLKLYGPNNLGEDMGIDYKALVIHQLCNAMVLVLFVSMVISFAIRDWITGGVIAFVVGINVVIGMFQEYKASKTMNSLKSLTSPTATVLRDGKSITVESQTVVVGDICEVHVGDSVPADLRLVSASNFETDEAMLTGESLPVAKDYQAIYDREKDTPVGDRLNLAYSSSSVVKGRARGIVVRIGLDTEIGKVASSLRAKKSLISRDPAKSWLVNCWRTVYNTAGAFLGTSVGTPLHRKLSKLAVLLFLIAVVFAIVVMASQKFNVDRGVAIYAICVALSMIPSSLVVVLTITMSVGASVMVSRHVLVRRLDSLEALGAVTDICSDKTGTLTQGLMVPRQVWVPRFATISVDGKGKDTSIKAVPRLSPHEYSHNENEDVGILQDFVSDYHDAKLPEDMSPELFSQWLDTAALANVAKLHWDEEKEDWSGQGDPTELAIQMFVAHMGGRDARLQRFQHIEEYPFDSTIKRMSAIFEDTQDGSRCVYTKGAFESVLDRCKYWYGGKPDAMTPLTDKDIETILANVDSLSCGGLRVLAFAQKKLSPEDKNSVPQERSQVESDLCFLGLIGIYDPPRPESLGAVKKFHRAGINVHMLTGDFPGTAKAIAQEVGILPTNLYHYPKEVVETMVMTGSQFDKLDEQQIDELPVLPLVISRCSPQTKVRMIEALHRRGRFCAMTGDGVNDSPSLKMANVGIAMGLNGSDVAKDASDIVLSDDNFSSILNAVEEGRRMADNIQKFVLQLLAENVAQVLYLILGLAFQDEDKKSVFPLSPVEVLWIIVFTSCFPAMGLGLEKAAPDLMERPPNDSRTGIFTWEVIVDMIVYGVVMAASCMGSFTTVLYGKDAGNLARNCNDRYSSACHDVFRARSAAFATMTWCALVLAWEVVDMRRSFFRMHPDTDQPCRQFFRDIFENKFLFWSIVVGFAGAFPVVYIPVINQVVFLHKAIGYEWGIAIGFTVAFWIGAELWKFAKRVYFKGQWKAQDPERDLEKNAIRDPFEAYSTSTTMLTDSNLKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X729</accession>
    <name>A0A8J2X729_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_06106g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_06106g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X729"/>
    <dbReference type="OrthoDB" id="288726at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11592">
        <property type="entry name" value="Agmatinase_PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF28">
        <property type="entry name" value="HYPOTHETICAL ARGINASE FAMILY PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035170869" evidence="2">
        <location>
            <begin position="18"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF88269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="419" mass="45640" checksum="A3D0339B504DE88C" modified="2022-05-25" version="1">MQLSSFLVGLTIFGAHALVLPQQQYQISRPLMEQLYGQILIDREGSEVNLESVHMLVEPSADDDEEFQARCGFYNENPVQFPGDDFDSDLIVFGGIVTFAHLPVARCFGANADEFDIAIVGAPFDTATTYRPGARFGPNGIRQGSRRLVSGLSPVRGFPGSKLRHLDPYHSGYKLVDCGDVPMTPFDNRIALNQLYRGHRRIHNTTSVKDPDQPPRIITLGGDHTVTLMNLRSAYENWGPLAVIHFDSHIDSWDPKILGGNISKSAALNHGTFLHYAHEKGYLADNSSIHVGIRSPFIAPGDEKHDFECGFEKIVARDIDIIGVRGIVNRVKQHVGDLPVYLTLDIDSIDMAVAPGTGTPEPGGFSAREILTILDGLEGINLVGADVVEVAPAYDTNGDITTIIAASVVDSLLGLMTVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X754</accession>
    <name>A0A8J2X754_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S03-05534g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_05534g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X754"/>
    <dbReference type="OrthoDB" id="1723809at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003876">
        <property type="term" value="F:AMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006329">
        <property type="entry name" value="AMPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359:SF7">
        <property type="entry name" value="INACTIVE DEAMINASE YBR284W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19326">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF88943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="807" mass="94765" checksum="AF19C00DB9C47966" modified="2022-05-25" version="1">MAISLLLDDYEHALWDHHTSKASAHAVLREQLEHSKEAVPSKNLSLDDMDMLPLWTQFDDQMNVGSPMYLEFPGENGGQGLIAAHFTNHESHSSQPVPCDASNFHLEAFEGDENPFQKLSDLRIKYILSSSQDSKSNIKYNVDKWFLYPQPLPKFWKYDHDERLKDEDSSEGESSDDCKKHRYHRNHYTGEFFELDQYKKEFRHHMLQYGSQMSQAMYNVPLFEEFQRDFTFAVKIAQNEQLARLSMRRLRYLNEKFELFQDLNAKAEKIENKLVPYRDFYNCRKVDCDFHLSGCISQRQLSEFIWEKLNSEPDRVVYETQEGRKLTLSQIFEVGRSEDEPVFIGLKVVDDQFLEWYKHVYLSSLHVIPHSELLKLLEGETLRFYILAKTFLEFDNYIEGEYLAEIVIDHVITNLEKSKYQTVQASVDFQFHRSGEPSWWERFAHWLVKWNVVSYNVRWNVQIKRIFTKLYHNGKVRNFQEFLDAIFQPILDKKSLENVELQYLLANLLNFDVVVSHTDDFLWRAFPNVFTKPKDWEAKGDNPTVMHYMYYTYAYLAQVNCMRYNNSLNTFSLRNSCSSLNNRTSQAGITLNFNDQIESIVCNMLLCNGGLLQSEHLWNSQPTMSYLYYLFQIPVLATPLSSVSMISTAPQEELKRYRAAMGQKQVYSIIQSKDKTYRGNPFLTMLKVGMKVSLSSNSVLFNKSYTLDPIIEEYSVAASIYLLEAADVCELARNSVICSGYEGFYKAHWAGLKVQKSPFEHEVIGCTETWYDTEENTSHRHNVPIIRRLFRKDTLDQEWEFILDQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X762</accession>
    <name>A0A8J2X762_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dynamin GTPase</fullName>
            <ecNumber evidence="2">3.6.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_08746g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.5" evidence="2"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X762"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000002">
        <property type="entry name" value="Dynamin-1-like protein isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF235">
        <property type="entry name" value="DYNAMIN-RELATED PROTEIN DNM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="14">
        <location>
            <begin position="25"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="13">
        <location>
            <begin position="693"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="571"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="658"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="595"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF88374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="780" mass="87338" checksum="488DC97030E671C1" modified="2022-05-25" version="1">MASLEDLIPTVNKLQDVMYDAGIDTLDLPILAVVGSQSSGKSSILETLVGKDFLPRGTGIVTRRPLVLQLNNIAPDSPLISEYVPNDTTEGVEENGESEMTLEDHLRKNNKSYQPPTKDEWGEFLHLPGRRFYNFKQIRQEIANETARIAGKDKGISKVPINLKIFSPHVLNLTLVDLPGITKVPIGEQPPDIEKQIKNLILEYIATPSCVILAVSPANVDLVNSESLKLAREVDPHGKRTIGVITKLDLMDSGTNALDILSGKLYPLRLGFVGVVNRSQQDIQLNKSVEEALDNEQEYFNRHPVYRTISGRCGTRYLAKLLNQILMSHIRDKLPDIKARLNTLIGQSEQELASLGDTGVITNENRAGLVLQLMNKFATNFISSIDGTSSDISTKELCGGARIYYIYNNIFGNSLKSISPTANLTVMDIRTAIRNSTGPRPSLFVPELAFDLLVKPQIKLLLDPSQRCVELVYEELMKICHNCGNTELARYPKLHSMLIEVVSDLLRERLGPTRSYVESLIEIHRSYINTNHPNFLSATEAMADIVETRKRNGELIARQKLQQKREFLQKEIQKEKAPDVAMESNSDSGKAESDSTADSESDEDGENADMKHSKDSFLKYFFGKEQNSQGGNSAFSNSQNQNRQQYAIPNDINGQTLVDMNNLSINGGGVQNTSDDGEDNKEPSLTEREELECELIRRLIVSYFDIIRQMIEDQIPKAVMCLLVNYSKESVQNRLVTKLYKESMFDELLMEDQALAQEREKCVRVLETYRKASNIIGGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X763</accession>
    <name>A0A8J2X763_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_03708g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X763"/>
    <dbReference type="OrthoDB" id="18472at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000280">
        <property type="term" value="P:nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21776">
        <property type="entry name" value="MobB_Sid2p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05600">
        <property type="entry name" value="STKc_Sid2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000141">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000319">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000109">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF417">
        <property type="entry name" value="CELL CYCLE PROTEIN KINASE DBF2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="178"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="13">
        <location>
            <begin position="479"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="23"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF88865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="573" mass="66130" checksum="D26EA086595B0CAB" modified="2022-05-25" version="1">MSLRPGGEVAALTDDISNFSFDQGGHGLKNPLRTTMLPTTPLRNKENGYSGTPSPTKSTVFLAEDSSTMDIDEPPREPIDPTSSPKKLPRGFHEKASSHKTQRLVSVCQMYFLDYYCDMFDYVISRRQRTRKVLEYLSQQKTLKNTNNDTLNHEWTGYVQKEHEILRKRRLKPKHKDFEMITQVGQGGYGQVYLARKKDTKEVCALKILNKKLLFKLNETNHVLTERDILTTTRSEWLVKLLYAFQDSESLFLAMEFVPGGDFRTLLINTRYLKSAHARFYISEMFCAVNALHELGYTHRDLKPENFLIDAKGHIKLTDFGLAAGTVSNERIESMKIRLEEVKNLEFPAFKEKSIEDRRSMYHRLRQTEVNYANSMVGSPDYMALEVLEGKKYDFTVDYWSLGCMLFEALVGYTPFSGSSTNETYENLRHWKQTLRRPRLDNGKAAFSDRTWEIITRLVADPINRLRSFEHVKRMSYFAEINFETLRNMSPPFTPQLDSETDAGYFDDFTNEADMAKYADVFKRQNKLSAMVDDASVDSKLVGFTFRHRNGKHGSSGILFNGSEHSDPFATFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X7J9</accession>
    <name>A0A8J2X7J9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S03-11034g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_11034g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4036563at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="780"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="857"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="161"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="536"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="599"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="743"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="780"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="916" mass="105868" checksum="86E8EF6AEF67B38C" modified="2022-05-25" version="1">MSNFFRDNLLGFKPRSNVFSKLRVKEVESQNEDSNARECDSSLENLFLTSNRDEDPASITDINSDVKIGSQFQMGSSTPMAVKKGNMFGRGLLDEDDLEITEVRDVAITEDIAIKPNSATANDCIDSSSQLITLPLNKDCNMDTSSNDVLLEAFTNTQKICSNLKQELHRVQTENSRLKSQMKNHQNDKEKIEEKFSEYKSLLDSFGNKSKALFEQKRQEDIKLKEFKENYEKLVKKIEGYRNDVKELKANLSQLKFLKKDSDLELTRRIKEIEYLKRDLDACSGQLSEEKLKNSSLVQEFGKIRMDLKESFSQGHSLVLEKFGLFEKNVIDAYRATIGEVSNSSTSGLTESIQAVQNSLISEFKFAFKESNKIFLDNQESQQQVLKQLHQIERGYEAIKTSSKKSTELQHTELKLQTKYLKDEMQKSQAEYCQKMAQWVETNQSISRTLNEDLQKAHLDSSKLTRTMSREYKSISEIIKQEIQKGENSTSKAMQKLIHEHSSFCQEIRSGHAEDGERIKNVIQQLTNFKDDLSNSRECETKIADLQAEISFLQLQKSQAISALGTKEAQYEDTYKKLMKKETEILRFEGLEKKLQDKIEFFSTQANEQQSKYSRLKEENITLKANSENKLVVQNEMLKAFQTENDSLKHRTAQLEEMRQHFERENSSRLDKIQKSNEQLQKLNVEMVQLKAHELELEEENRNLKKSINENQCQFEETSDDFKRLRQKVIVLESDKQDIVSEKMDLYDQIEKLKGVISNLKKKVALLQRNEEHYVKRIGHEAAELRGREKQSERQTSPRKKLKSSSGNMLKSQPPGMESETRNNFENKIEEDEFDLSSSLNDDLELTNPSPIKLVTARRGDTKMRPPASSRKKLLLLDESDSVPSKHRXXXKKKKKRKNIFILKGIVSSYCYNVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X7Q2</accession>
    <name>A0A8J2X7Q2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S04-00386g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_00386g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X7Q2"/>
    <dbReference type="OrthoDB" id="5563016at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12751:SF18">
        <property type="entry name" value="PHOSPHATASE AND ACTIN REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12751">
        <property type="entry name" value="PHOSPHATASE AND ACTIN REGULATOR PHACTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="488"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="857"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF89274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1100" mass="123019" checksum="DFD1439881199D91" modified="2022-05-25" version="1">MSKNAHRNQSAPSLLNSSFYSSNSMNTLEHAKNFRNSLILRDSSDQSLSWSHQSDESVVSEEDIDQVVGSIVPSERTDRETITMQMKNSPSLSALAGILNEKSRQADEKARLSKVIDSSILREEEETGACSQDLSHDTSATGEWNQTSSPNLIDIHDTNSVTYPVVTTTNTSDYEQPDFLSTPKVHSRRNTNPFLQSFEPSRSSAQEPFTIQSSILEEPEFEQSSPPLKAHRNTRNRPKSTSILLDHSFDQGDKTAAVDQIDMQRYSMLHVPAVARDKSNGSIKALDQQQFDVGEDNQIQHSRQQSKSNVSGQPETKEKHADTLTTRLQSKGSNLSIPHSDEGNKAQSNSVSHNQRPRRHSRATTLEKTEIEGKRTHHRAHTIDHSYLGTNGPSALDQEKLKYEKNFFEDHLDELPKGRHLRNANCIANPSSTQRAVSAGSVLDQSSSNKRRSFFSLFKKKSSKDVTPKKSKQSSKWNQNSILSSNTFNSANAVPTAPSPEKLAKKSHSTNTIFSTFRKNKDKDDSALLTSTTPPRKESQLDAISKESSSFSSVKKEQPLENFTPTTNDNFDHPYKAESIAEEPIEKGQSFENTSFNSNKYTEEVPLEEPSLKKENSEEKHQQIFEESRQDSFSTHPMGESLLEGTHFDDPLIKDLPKPNLPFLIKHDAGEALFPKSLDKYEVESIVSLERSRSVKSNRSHRRSLTDTLSVNAQNEGMFVTEASPAILSTPDLSKSPTSSILRNGRFDSVSVLCNVEDKPEDSPMEKDSSEVIPKLSLSPNRSPIITIEKQFDLLTVEDINDDVEENKEIPKQINNRENTTGDDPEFTSEMIEFASLINFGDGLTLDLDVNSDVQSQKLQQDPLGGNNVPQESCESSSSIEEDVHIDAQSPDQEVAMVKSSLKQSSGSLDELQNAKLEDHINKDQIISANDLPESLLAEEPTDIPNELLEEIRSPSSQAFGQFHEGEDVHRQRHIFRDILASQAYAANDPSSRPVSMSFKGLSAPSLNQSASDWPLFESQDGKFQNPTVQESTSSHHKVNFSSKILLYDTYSLEEYDRKPEIATCNMLTPQSAHMIKAELNQLKSEMEVHEASRCYTHFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X7Y8</accession>
    <name>A0A8J2X7Y8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ZYBA0S04-06700g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_06700g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X7Y8"/>
    <dbReference type="OrthoDB" id="4061518at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF89538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="806" mass="95160" checksum="86FDCA1387FF8B35" modified="2022-05-25" version="1">MLRKFSIRYSTARILWNLETYSRLHLSYSRPFSCLGPADNQQGKDDIANWVRSSLNEPIPSQHFPRAVGNQVLNPNRTSHLCPLIQVVSEEEFCEEWYLRFRRGDSEVVNTVLKKIREDRVFFRFGQIEMLLGTLKYLRRNFDGHKIFCAYRDFLPYLRQDDDDNEAYCKFLETTLRIENSLANYKICEELFSEYIKFPNIKPEVITVGLRSFLENNNLQLAREFYMQALSNPDAFPMGDKQLTELLRLLSDHREYRSVRTFFNLWLKKKCQAESDGTDHQPSFSALSLVHRTYLKTNDKDGLVDFLSQDVVQKTGYPWDVSRELNDFYHALHCGSLILQSDIETRIQEFAAKLSSNQKKREEFYLFLLNSFALSKDFANLKFVLCLIQKDCEIELKPKFHLAIARYFVNQGMLHNLLEYYSDVLRRGGPDCLQLRVSIINQLCRCASYSHPALTKEIINEFKVILSRDMYITEFPQLRQFLRKLSRIRLKGIHGNEEFGILEMSQVDYDRLEIFEKFVCRGDLAGARLFILENLRQGIQPSFNFHYCILQKCLNLQLPSLAKTLDDLFSGVYKEVPVKLKILWLRYEILTQYNTLLTHEELFSVAKIKVLASRLQDFERQYQSALNFRSYMQLTQISLSIREYTTSELLLQRAISLMDERDKRHWQIYYLTSLKLAARIYDMNTFLKLVNEWIENSRASWITHDCLRQVKSFAKLFAKREDSIPNYDENLRLEIASSCERLVHKYTSLKFEGLDSMRVLSKFLKDWLDMEMRYRAKLLKSRRAELETQLSEESANASGIEIDNYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8I7</accession>
    <name>A0A8J2X8I7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">dihydroxy-acid dehydratase</fullName>
            <ecNumber evidence="14">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">BN860_15082g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:27695"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IlvD/Edd family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="14"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8I7"/>
    <dbReference type="OrthoDB" id="3851628at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.80:FF:000001">
        <property type="entry name" value="Dihydroxy-acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="entry name" value="IlvD/EDD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050165">
        <property type="entry name" value="DHAD_IlvD/Edd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056740">
        <property type="entry name" value="ILV_EDD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="ILV_EDD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002068">
        <property type="entry name" value="PRK00911.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000:SF5">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24877">
        <property type="entry name" value="ILV_EDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="IlvD/EDD N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroxy-acid/6-phosphogluconate dehydratase N-terminal" evidence="17">
        <location>
            <begin position="53"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Dihydroxy-acid/6-phosphogluconate dehydratase C-terminal" evidence="18">
        <location>
            <begin position="389"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF24877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CDF87814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="583" mass="62580" checksum="9D9FCFA57315358D" modified="2022-05-25" version="1">MGLLVRASRQFSTTQNVAKKLNKYSYIVTEPKDQGGSQAMLYATGFKPEDFHKAQVGVGSCWWSGNPCNMHLMDFNFRCAASVNKAGLKAMQFNAIGVSDGISMGTKGMRYSLQSREIIADSFETQMMAQHYDANISIPSCDKNMPGVMIAMGRHNRPSIMVYGGTILPGAPTCGSTNLPPRIDLVAAFQSYGQYLSKEITETERADVVSHACPGPGSCGGMYTANTMASAAEVLGITLPNSSSFPAVSQEKLSECDNIGEAIKKTMELNILPRDIFTKEAFENAITYVIATGGSTNAVLHLIAIAHSSGVKVTPDDFQRISNKTPLLGDFKPSGKYVMADLIKVGGTQAVIKFLYDNGFMHGDTLTVTGDTLAERAAKSKPLAEGQDLIRPLSNPINPRGHLQILYGSLAPGGAVGKITGKEGTYFKGKARVFEEEEDFIHALERGEIKKGEKTVVVIRYEGPKGAPGMPEMLKPSAALMGYGLGKDVAMLTDGRFSGGSHGFLIGHVVPEAALGGPIGLVKDGDEIVIDSNNNKIDLLLDDKEWAERRAQWSPPAPRYTRGTLSKYAKLVSNASQGCVTDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8N6</accession>
    <name>A0A8J2X8N6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ZYBA0S05-06964g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_06964g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8N6"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10233">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000001">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000016">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="551"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="607"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="509"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="553"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="610"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF90016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="642" mass="69423" checksum="0B52693D384FE8D6" modified="2022-05-25" version="1">MTKAVGIDLGTTYSCVAHFSNDRVEIIANDQGNRTTPSFVAFTDTERLIGDAAKNQAAMNPSDTVFDAKRLIGRNFNDPEVQGDMKHFPFKVVDVDGKPQVQVEYKGETKNFTPEQISSMVLGKMKETAEGYLGENVNDAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKGQEENVLIFDLGGGTFDVSLLSIEDGIFEVKATAGDTHLGGEDFDNRLVNHLANEFKRKNKKDLTGNQRALRRLRTACERAKRTLSSSAQTSVEIDSLYEGIDFYTSLTRARFEELCGDLFRSTLDPVVKVLRDAKLDKSAVHEIVLVGGSTRIPKIQKLVSDFFNGKQPNKSINPDEAVAYGAAVQAAILTGDQSSKTQDLLLLDVAPLSLGIETAGGVMTKLIPRNSTIPTKKSETFSTYADNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGVPQIEVTFDVDSNGILNVSAVEKGTGKTNKITITNDKGRLSKEDIEKMVAEAEKFKEEDEQEANRIQSKNQLESIAYSLKSTISESGDKLDPADKETVSKKAEETISWLDSNTTATKEEFDDKLKELQEVANPIMSKLYQAGGAPGAAPGGAPGGFPGGAPPAPEAGGPTVEEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8Z6</accession>
    <name>A0A8J2X8Z6_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Elongation factor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_05248g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF89940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8Z6"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01681">
        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04096">
        <property type="entry name" value="eEF2_snRNP_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01885">
        <property type="entry name" value="EF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16261">
        <property type="entry name" value="EF2_snRNP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03700">
        <property type="entry name" value="EF2_snRNP_like_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1430.10:FF:000003">
        <property type="entry name" value="Elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000010">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000006">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000003">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000058">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF10">
        <property type="entry name" value="EUKARYOTIC TRANSLATION ELONGATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="17"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF89940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="842" mass="93290" checksum="8076B09A1DF342F3" modified="2022-05-25" version="1">MVAFTVDQMRSLMDKVPNVRNMSVIAHVDHGKSTLTDSLVQRAGIISAAKAGEARFMDTRKDEQERGITIKSTAISLYAEMTDDDVKDIKQKTDGNAFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDTVEGVCVQTETVLRQALGERIKPVVVINKVDRALLELQVTKEDLYQSFSRTVESVNVIVSTYADEVLGDVQVYPSKGTVAFGSGLHGWAFTVRQFANRYAKKFGVDKNKMMERLWGDSYFNPKTKKWTNKDTDADGKPLERAFNMFVLDPIFRLFAAIMNFKKEEIPVLLQKLEITLKADEKELEGKALLKVVMKKFLPAADALLEMIVMHLPSPVTAQAYRAEQMYEGPSDDQFCQAIKRCDPNADLMLYVSKMIPTSDKGRFYAFGRVFAGTIKSGQKVRIQGPNYVPGKKEDLFIKAVQRIVLMMGSRTEPIDDAPAGNIVGLVGIDQFLLKTGTLTTNEAAHNMKVMKFSVSPVVQVAVEVKNANDLPKLVEGLKRLSKSDPCVMTYINESGEHIVAGTGELHLEICLQDLENDHAGIPLKITPPVVAYRETVEGESSQVALSKSPNKHNRIYLKAQPMDEEVSLAIESGKINPRDDFKARARIMADDYGWDVTDARKIWCFGPDGNGPNVVVDQTKAVQYLNEIKDSVVAAFQWASKEGPIFGEPMRSVRVNILDVTLHADAIHRGGGQIIPTMRRATYAGFLLAEPRIQEPVFLVEIQCPEQAVGGIYSVLNKRRGQVVSEEQRPGTPLFTVKAHLPVNESFGFTGELRQATGGQAFPQMVFDHWSTLTSDPLDPSTKAGGIVAAARKRHGMKEEVPGWQEYYDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X969</accession>
    <name>A0A8J2X969_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_08768g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_08768g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X969"/>
    <dbReference type="OrthoDB" id="286301at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050904">
        <property type="entry name" value="Adhesion/Biosynth-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900:SF125">
        <property type="entry name" value="FAS1 DOMAIN-CONTAINING PROTEIN YLR001C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900">
        <property type="entry name" value="PERIOSTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035263795" evidence="2">
        <location>
            <begin position="20"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="754"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="3">
        <location>
            <begin position="30"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF88375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="810" mass="90935" checksum="1D104285F797BB69" modified="2022-05-25" version="1">MMILLHFEILLLLLGVVFSSNFEAQDDFPFTSVVDILSQNVEFSTFLRAIQRQGYIPYLNELQNYTLFAPVNSAFIEEGVESEIDIQNYLLHNTILPSSDIDDGIHVISENVKFPFILERTEDDLVSINNATIVDPDLSPNFQKAIVHGIAGMLSTPPEVEKFLGQLEKEFNDFHFFNNFIRNMSPLASLVLNRTLLLPSDANFRNFYSDVEINYIIDVFGSLQKLNGKMGHVWYTDRQRFFQNIILEDILGGFIENSTSSQNLNGKVVNFESNQDGSVISANGSISSDLSNMLFSRGIAHAFNNLRIISNVVQFNAEKYLHGLNCTNFVKELYFRNLQHLILDGNLGENLTIFVPTMPADGSDGFSKPNLLYHFAGDEIRLDKEFAVTSSKASKMFESEYCDSNAKLGRNCQRIKITKDKNEYYINEKCKIGHKEPYQVGKTLIYMISENLKLPGDLASSINPFYGCSSSLMFLNQLGLLDLPLNGKGYTILLPCFNSWESLELNLEFFQKNATAINLMMKNMIFDGLIYSDFKDSRFHAKNLLGESITVKAEHGGCDEDDDPSLELSSMKGKIKIERSLDLFFNQGVVHPLKQLNLPESVTISLKDLIRTAGSNNLIGLLEKFEELSSIVNNNEPYSILVPTASSLIGGILNSTKLIDFLKLHVIPGKYTSGLLNCCGNVSTALGDKLTCRRDTQGNQFLKVSDGSQNEVRILKKGCTSFHDDSCVFLIDRPISLQWLNHGKSRLRLPHSPLAIGIVVETVVLIVCLAIFLYRKRRKPAWQLINPSDDADITRPLVEPSVRDAETGAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9F3</accession>
    <name>A0A8J2X9F3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">BN860_13014g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_13014g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tyrosyl-DNA phosphodiesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X9F3"/>
    <dbReference type="OrthoDB" id="47785at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017005">
        <property type="term" value="F:3'-tyrosyl-DNA phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010347">
        <property type="entry name" value="Tdp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415:SF0">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06087">
        <property type="entry name" value="Tyr-DNA_phospho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="11">
        <location>
            <position position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF88557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="522" mass="59487" checksum="CE9B0099C686E805" modified="2022-05-25" version="1">MVSDARKRVAERWSKIDYRSGSIDENGYSNKRSKPDIIDLTLSDTEEIAETSEHRTQRPSSEVPEVPVGNCPFRIIKSDVFDSGGGKSQYFTDLNEIFNDPTLHKTFLFSFQYELDFLMGHFHHNVERIVLVAQEGTVLSPTTPQALSLVPKTLLIQFRMPPFTCHHSKLVINIYEDGACRIFMPSSNFTYAETNYPQQVCWRSPRLRAVTNPPTVAFQQDLIRYMDSYNSPDIQKYITPCLKNLNFEPLNDVQFIYSTPSKNYVSGLRLLAAKISSLKQDNNKNLTHHYLCQSSSVGNSLSKKTSVNLFTHLMIPVLDGMIPADSKNTPETSRLLQLYKSRSVVPYLLYPTVQEISSCPAGWLCSGWFNFNYNRDLNHFNMLKKQFKVLYKQDNAVTSKQRRATPAHSKFYLRSSTSAVNEAPFKKLDWCVYTSANLSYSAWGKTRAKPRNYEVGILLTSPNLYCQSFADLVYKGKSTSLPQGVTVAVPWTTSLEPYGDNDEAFCISKEYEMLDCNGRRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XA73</accession>
    <name>A0A8J2XA73_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">eIF-2-alpha kinase GCN2</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">BN860_11078g</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="341578at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004694">
        <property type="term" value="F:eukaryotic translation initiation factor 2alpha kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140469">
        <property type="term" value="P:GCN2-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990625">
        <property type="term" value="P:negative regulation of cytoplasmic translational initiation in response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009893">
        <property type="term" value="P:positive regulation of metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14012">
        <property type="entry name" value="PK_eIF2AK_GCN2_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23823">
        <property type="entry name" value="RWD_GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14046">
        <property type="entry name" value="STKc_EIF2AK4_GCN2_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000856">
        <property type="entry name" value="eIF-2-alpha kinase GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001061">
        <property type="entry name" value="eIF-2-alpha kinase GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000050">
        <property type="entry name" value="eIF-2-alpha kinase GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000379">
        <property type="entry name" value="eIF-2-alpha kinase GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016255">
        <property type="entry name" value="Gcn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024435">
        <property type="entry name" value="HisRS-related_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF136">
        <property type="entry name" value="EIF-2-ALPHA KINASE GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12745">
        <property type="entry name" value="HGTP_anticodon2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000660">
        <property type="entry name" value="Ser/Thr_PK_GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0010">Activator</keyword>
    <keyword evidence="10 20 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 20 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="13" id="KW-0346">Stress response</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="5" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="RWD" evidence="24">
        <location>
            <begin position="17"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="218"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="595"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="131"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="719"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1505"/>
            <end position="1529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="138"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="720"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="19">
        <location>
            <position position="829"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="601"/>
            <end position="609"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="624"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="625"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00073598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000660-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000660-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="CDF89181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1651" mass="188447" checksum="3BB47C4E1A8A8843" modified="2022-05-25" version="1">MSRTHLSLDEYYEIQRNELEAIQSIYMDDFKNLTKQKSSWDKKPQIVFEISLRSTIKEPLESSLTLHIALTPLYPHTAPDITFKNVKNVMDSQLNLLNEEFAKIHEKSKDQENIFEIASLLQEKLDEFQHGANSQSLEDDRLQRIKENKERQEREEKERERQNQERRRSEQKVIDEIVQKELEKRQDDEYIVSDANSSMDLLPPQEWVSSGEAIVFNKIIRAKLPNNSFYRFRAVVNPKPIKKQTDLFSFAEQVLVKPYIPPDSPLADALMSSEMMENFHYLLTEVRLDNSHFNTSSGKKEISNLEKELESLLRVNHDNVNRLYAYTVERMGKNNSSFVWKVRFLTEYSMSYAVGDIVESVGFVNLATARIWMIRLLEGLEALHKFGLVHKYICFQTVSLIKDTDFGTTIPKLLHPTYGYTLINMLAKHPNRQGPSLDAPICPWQAPELTTTSMGKPQRQTDIWEVGVLFMQIINGVDTVLNFSSPQDFIETTNMDSSLRDFLAKMVAQDPKRRSSPLELLPMKFLRTNIDIGMNKLNMQSENTNSSVSRRSFTNSFALKSRTASQSSGRRRSFNVGSRFSSANPATTSRYASDFEEIAVLGEGAFGQVVKARNTLDSRYYAVKKIRHTEEKLSTILSEVMLLASLNYQYVVRYYAAWLEEDGSGNEQALVSEEEEDFDPDTEDLLNESSILRSRTARSFDNSNWDFISGSGYPKIEFADSTTSNDKPLSNVDDTSSSDEEFTEDHYSDGESESQNQLTKQEISTKNQAAINKKKSTLFIQMEYCENRSLFDLIHSENLSKQRDEYWRLFRQMLEALSYIHSQGIIHRDLKPMNVFIDESRNVKIGDFGLAKNVHRTVDLFRLGSHSGVGSSEHMTSQIGTALYVAAEVLYGKGQYNEKIDMYSLGIIFFEMVYPFTTGMERVNILRNLRTPQVKFPQDFDDNKMKVEKRIIKLLLDHDPSKRPGAQSLLDSGWLPVKHQDEVIKEALKSLADQSSPWQQQVRETLFSQSYTLTNDILFDNSQPSSTPFSQILNSKMIEEVTKVFRKHGAVPNDDPPSIFPKAPIYGTQNVYEVLDQGGTVLQLQYDLTYPMARYLSKNPNCVSKQFRIQHVYRPPQHSKSSLEPRKFGEIDLDIISGSSADSSFYEAESIKIIEEILTTFPAFEKTNSLFVVNHANILESVFDYTNIDKAQRPFVSRMLSQVGFTKTFKEVKSELKSQLNISSTSLNDLELFDFKLDFEAAKKRLHKVMVDSPYLRKVDESLLHISKVINYLKPLEVTRNVIISPLSNCNSAFYRNGIMFQAVYNSGSSKSLVAAGGRYDSLISYFARPSGDKSHNTQRAVGFNLAWETIFGIAQNYFKLGTGSRIKKRNKFFKDSTIEWKPSRCDVLISCFSNSILNTSGIEVLNKLWKSGIKADFLRDCYTVDDVVTGAQKDGADWIILLKQQSYTLPNHRRKYKPLRIKKLDSALDIDLDLDEFLNLYHQEVQSKELTNTSFSIGEIHDDAKKKEDGSSAGSSKEAEEDSVAGSTNQKVIFVPNPAIRSKKISKREKWVYEDGAKTAFNAIINSLTTAPVVTVDALRDETLEMISITSLAQKDEWLKRVFGSGNNSTPRSFATGIYNTLSKEAAKGNKWGILYCHKTGKSCVIDLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XA77</accession>
    <name>A0A8J2XA77_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ZYBA0S11-02168g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_02168g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316464">
        <property type="protein sequence ID" value="CDF91407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XA77"/>
    <dbReference type="OrthoDB" id="10250769at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="U3 small nucleolar RNA-associated protein 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000710">
        <property type="entry name" value="U3 snoRNP protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
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        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059157">
        <property type="entry name" value="WDR36-Utp21_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007319">
        <property type="entry name" value="WDR36/Utp21_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22840">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22840:SF12">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25171">
        <property type="entry name" value="Beta-prop_WDR36-Utp21_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25168">
        <property type="entry name" value="Beta-prop_WDR36-Utp21_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04192">
        <property type="entry name" value="Utp21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDR36/Utp21 N-terminal" evidence="6">
        <location>
            <begin position="51"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="582"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="WDR36/Utp21 C-terminal" evidence="5">
        <location>
            <begin position="713"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="771"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="771"/>
            <end position="794"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF91407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="938" mass="103823" checksum="839A3EA7C3C01F00" modified="2022-05-25" version="1">MTVEWAKKRKLESLDRNVISPSRVFSPFRVIGNVTNAVPFAVGTLGTTFYIVTSVGKTFQIYDANNLHLLFVSERETERPIVALATHFHYVFAAHGNKVGVYKRGICQSELELEDNVVIRSICVFGDFMCITAANNSVYFYRKNSSKDNYPTEYYTKFTVGKLLGGDIVSVVHLATYLNKVVIVTKSNLLLYNIRTGKLIYSSDEFPDEITVAEPAPALDTLALGTSSGEIILFNMRKGRKIRTIRMPQLKITSLSFRTDGSPHLCLAFSNGDMVFYDLDRRSRIHVLKNIHSASHNGVIRASFLNGQPIVVTSGGDNKLKEYVFDPSLSQANDEVVVQPPRFLRSRGGHSQPPSSIAFADAESHFILSASRDRSLWGFSLRKDAQSQEFSQRLHKKKDGSRIGGNTIKEKFPEIVALAIENARIGEWENIITAHRGEVVARTWDMRRKRVGRWALDTTDGGYVKSVALSQCGNFGFVGSSNGSIVVYNMQSGILRKKYKFHRKAVTGIGLDSMNRKMVSCGLDGLVGFYDFNQSTLLGKLQLDAPITAMVYNRPSDLFALALDDLSIIVIDAVTQRVVRQLWGHTNRITAFDFSPDGRWVVSASLDGTIRTWDLPTGGCVDGIRVESVATDLKFSPKGDFVATTHVAGNGICIWTNKAQFKPISTRTVDEEEFSLAALPNASITGGGSLLEGAFEGADEGGAGEFSSYQSVEQISDDLITLSLGPRNKMNTLLALDVIRQRSKPKEQPKKSNDVPFFLQLAGEVVGDEASKREGVISESREELRRKNETERNQDLLGEQVTNSKPTGLVGFESEFTRLLREGGEASNFTQFLQNLVDLSPASVDLEIKSLATAPPYNEIVWFISALTQGLCSNKNFDLYEAFMSLLMKAHGDVIHANNTDHELKKALNSWNAVHARDERLVNLVNFCVGVANFISST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XA84</accession>
    <name>A0A8J2XA84_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_11408g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">COPII-coated vesicle membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XA84"/>
    <dbReference type="OrthoDB" id="10256289at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF0">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="4">
        <location>
            <begin position="198"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF89196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="645" mass="72336" checksum="5E4D91FBD30555DC" modified="2022-05-25" version="1">MSWFNYSVLPASRKEALKEVGDDGHVHCLYQPLMGKQVSLQGPFKCSNCRGCYGASRIVESQDGKHWICVFCNTYNNWVADMPDCESYITKTGDKSKDQKLAIVVDTICDTNELQALQKSLTLDPQCKYALVTLQRNGDVTVHSPEGSRAFSSSHHKVELSLKKLNGDYFTKHLKDPWYDVKGVTAAFAKLVAKDVHGNKRAKRATGLALFLASLLADQVMCFLTGPCTEGPGKVVSKEKRQHMRQQQDIDKSTKYVKDASNYYSKLAPRASFAIFASSLDQVGFMEMSEILDTAAQFDSFTDARFDQSLQKYFQLRQTAITDVRLTVVTSERLLVDGVMGRASPLKPSMRNYSDTPRGVGNTNNWALESFCVDPSNLVVPISLSVLTGPTVRAAGETVPEQLCIQFQLKYTSDGGPYLHVHTQVLHTSNSPDCFVVKSFNPKIEIAYLMKVFAFRTINGRFSSDHLQRVRYSLDNEAIRRHQSQDCLKLFYCLRRTPLFTTRNVSPDERTLFLQQVLHSNIDLCLLYALPTVLQYRMGAVNSPLLSQDLLADPSPALIDGGNFVGVRYAKESTEVDLARKSAKTIASSRFPRPLFIDTKIGGSKDRFFKSKLIPMDEQGADLLETQDISLENFIRTVRERSKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XA92</accession>
    <name>A0A8J2XA92_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="15">ZYBA0S10-02784g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_02784g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316463">
        <property type="protein sequence ID" value="CDF91237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XA92"/>
    <dbReference type="OrthoDB" id="448399at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.10:FF:000021">
        <property type="entry name" value="MUTL protein homolog 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.10:FF:000024">
        <property type="entry name" value="RNA-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23111:SF40">
        <property type="entry name" value="RNA-BINDING PROTEIN INVOLVED IN HETEROCHROMATIN ASSEMBLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23111">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="210"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="14">
        <location>
            <begin position="339"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="14">
        <location>
            <begin position="469"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF91237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="564" mass="62008" checksum="FF60A14B1B0AA692" modified="2022-05-25" version="1">MYYVVLEVETTHLIETPKDQCKIAKISYQFVNADTLQTTYDDDQVPIITLSEAGSPSLQEAVTKLDQDIAKVTGDDPFVLCSLNSTWHVRVTLPRQARDHGFVLPPHLQHPKIFDLWKEYERWCVNHPEVMPLRKNGGAGGKRIKDLAELARVLDLPKPNLADSFDLPRSVEVLLQLHKKCTTQEDMSMVLTRPYDSHTDTRDFLQEYSKVLYLNNLPPDTTQSELESWFTQFGARPVGFWTVKNIVEETSNVNNNWSMNNSPYVEEQDSISGFVVFQTHEEAAEVLILNGRSILSNMANTKQPRVVEHIVEIQPSSTRVLDRAQEILSPFPQSKNKPRPGDWNCPSCGFSNFQRRTACFRCSFPAATAVQVNTKQYINSTGPNLQGGKITPSPSQSYYPLNVSQSQGMLAAHGRGMDQDMVMMRYQNGGNGLNFSQNGATNGGGGGGNRYNGSHGGNMGNGGSNVPFRAGDWKCPACTYHNFAKNVVCLRCGGPKVTAVDLENVSGSSAGAPVAKNGSVSLLDLASMGSTLSLGDKLSSNHATAAHDGFGVSLDSRSVSEPKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XAT4</accession>
    <name>A0A8J2XAT4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ZYBA0S04-09318g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_09318g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CYC8/SSN6 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XAT4"/>
    <dbReference type="OrthoDB" id="418911at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001929">
        <property type="entry name" value="Cyc8p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000078">
        <property type="entry name" value="Transcriptional corepressor Cyc8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000163">
        <property type="entry name" value="Transcriptional corepressor Cyc8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051630">
        <property type="entry name" value="Corepressor-Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017:SF1">
        <property type="entry name" value="LD02225P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4 9" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="83"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="117"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="153"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="190"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="227"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="299"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="333"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="422"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="601"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="665"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="601"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="665"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="700"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="711"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="773"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="811"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="832"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="874"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="899"/>
            <end position="910"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF89647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="926" mass="102046" checksum="2E1F21AE09CCB950" modified="2022-05-25" version="1">MNGGIQTVLMTPGQQKLQPPGLEQQQQQQQQQQQQQQQQMLDPLTQATAETWLSLASLAETLGDADRAAMAYDATLQYNQASTKALTSLAHLYRSRDMFQRAAELYERALTVNPELSDVWATLGHCYLMLDDLQRAYNAYQQALYHLSNPNVPKLWHGIGILYDRYGSLDYAEEAFAKVLELDPHFEKANEIYFRLGIIYKHQGKWNQALECFRYILPQPPAPLQEWDIWFQLGSVLENMGEWQGAREAYEHVLLQNEHHAKVLQQLGCLYGMNNVQFYDPQRALGFLSKSLELDPSDATTWYHLGRVHMIRSDYTAAYDAFQQAVNRDSRNPIFWCSIGVLYYQISQYRDALDAYTRAIRLNPYISEVWYDLGTLYETCNNQLTDALDAYKQAARLDPENMHIRERLEALTQQLANPNTAAALANGGGTAPNGSAVAPAPPPPIMLQPTLQFNDQVNPLNVRPPAATMYANEPLGQQQMQQMQQQAQVQAQAQAHAQAQAQAQAQAQAQAQAQAQAQVQAQAQAQAQAQAQAQAQAQAQAQAHAHAQGHMLNAHYPNASVLPQIHAQGETALHEHPSYPQQIMQQPVVPPAMSPAAQPVQTVQQHMQQPSQTQTGTAHTTTSSSPVLPQKRHMDGSIHTLVNAAVPSVGENSTSNSNAASVPTLLTSQKANEPQSKKLKVKKSPKNSVANLKIKPSTIKGKEKEKEKLAENSSVNSVTSGGDAQSPNQEEVTPSEPSLAKLDGVGNHSAHSETADVPTPKIERAAPAVSPISTESTQPQATSPTQQVSSPVVKPMTAGDGNESGSAVPEKTTKPESPAEQRETRKEGELSTPVSENDNGSTAQDAPITSATSEQTDAASHRDAETVIKPSAEAIEKLQEEAKLREEEEREKLEQEQLNSKSQSENNNLARENLVRQVEEDENYDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XBD8</accession>
    <name>A0A8J2XBD8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ZYBA0S06-00870g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_00870g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XBD8"/>
    <dbReference type="OrthoDB" id="2943660at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF12">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="14"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="53"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="872"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="945"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="989"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="879"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="945"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="995"/>
            <end position="1005"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF90114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1116" mass="126099" checksum="0F383EF8AA5E0057" modified="2022-05-25" version="1">MCGDVDFKKLAGKSCLVCRRRKVKCDKTKPVCLACVKHRSTDECIYDDGYKSASPKGTGLSKSLTTISGSTKASTDSSITKPGSSRTSPLSSSQPTRFSHSNRSRVLAEIESLRKNLATLEKLLESNYVDSDFREPDDDPWFEALRGFPPMSFCDYEMVSMRDGSLAFDGPLSYRAVALKDPFLAMAIKRGHLQGTLEINQTIKRRKVMHIQYGSELSSDHPHLDAASRMRDEVKNTSNENDDQQVSDVSEVLKGHLRQNLKQINKNMNQENLETGKPLSMKHILNETEGNEFIDSRTRQKSAEQQESCSPGSDITLWKNIKKILDNLETLRSSNLKYELSRAVLPIQENMFGYSSTWQKNPEQEILVRIQSLLPPEKAVWMQIDNYFKCPIHGLYPVISEKWFRENVETIIGPNRNRDVAPIVSVSERLDFAHLGILMCVLSLSHMALVESTSQNLTKEEKYLLSHPISKECMDAAQLCMDIFKLLRKPVLPVFYCGLLIRIFRRFARDEGDSIEAEDTSSFTGLIVEVAYGLGLNTDLEKSSQFAEHEVYLHQWRKNWYLVFLLAMDEALTAGNCLSIADKSYATKLPSINMDDNGNFPSFLTNPSLEIASVNCMRSNYELALVHHKALKYVINKSEMIGCGEIHKMLDELESATAAACGSSLATILAKPAGNLEDSVVKLGAFRGFIRSKAFILLLTTALFIHLDKMETDGSDIHQSKVSISYMKKLVSMYVDLEPLLVLLFFGYDNTIDNVHRIFGRASEVIVMQACVEFLAKFVPVLYLLISRCLHLKFTFLNKKKDESNFEVKSEEHKILSSLEAVVNKGMDKLSHINAIISKLSRHNFQVWRISKSSMSMYNLLKEQSGMFEKCAKGSQNEKGVDDQETEKQETENSPVDLASYLPNSNTFRKANSSDWQEIYTLLCYTNYNIFKPYIKEQHLRMARDSRVQSERNSDSESANMEGKEDPREVCEPFSAEFIARITQNAELPGAALKSQRTGTSSSDENPQRVRKLTAVGWLEVERMSNFDDIDQLWNESILRTGEANDYTVHGSSSSGMGHTGFNSDVHLPKSAESPSNNSHLAMTSEELLKNQIIQQDHIIKQLQQLTSEQLHKLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2SX38</accession>
    <name>A0A8J2SX38_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BN860_05050g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_05050g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2SX38"/>
    <dbReference type="OrthoDB" id="5579281at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902657">
        <property type="term" value="P:protein localization to prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040345">
        <property type="entry name" value="Mug56/Spo71"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039486">
        <property type="entry name" value="Mug56/Spo71_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057379">
        <property type="entry name" value="PH_SPO71"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029217">
        <property type="entry name" value="Spo7_2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28076:SF1">
        <property type="entry name" value="PROSPORE MEMBRANE ADAPTER PROTEIN SPO71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28076">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN 71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15404">
        <property type="entry name" value="PH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23207">
        <property type="entry name" value="PH_SPO71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15407">
        <property type="entry name" value="Spo7_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01316">
        <property type="entry name" value="Spo7_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sporulation-specific protein 71 N-terminal" evidence="4">
        <location>
            <begin position="34"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="1052"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="215"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1179"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="128"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="143"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1179"/>
            <end position="1194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF87379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1248" mass="143157" checksum="67EB8E7D837BBE06" modified="2022-05-25" version="1">MTLTTKEIISIVFNDDQYIQHVNSHSLGENAHWKVIEIPRSSFTAFRLSYASPIELSLSSKTVLLGGIPSQWYHAQRNSIRNCLGKMKNSKLREKMGKESDYGIRAIYHEDKSAVEQPLGESSKDTQRSTNCRKSSVTGPRQNPKEFRHASTTTVKDRICSLENKSQTSPFLPAIDFYRIASPGERPLLQREGYTSGDLPTVPLESTNVLRNWLTRNKDREDNAQDTAERSGKNDNHTIKNGDTSFDSSVEGLGAPYDNLREFSGLKDREKTLDQLYKEQEQNEKEMNRLLNTTNAYWDETKKDGQSDETYVSVITDPSDVKETERSAVPAASTTLPYTEPTISANEDAKVFESFNDRVNQSISFGIPLHLKGQKALIDDALESGLNDAQSTKRRVVFLKALASSKDFKKLSVLAPEPEKVPLSKVESHTRHEHSSNYKLHGRRLKSANVKTKGIFSKFKQRASKKCGTYYSSHGHSDRKGPVLMMDKMLVMVKTAVNQRNPILDFSESEPLDTRVADRWREYIVIARQTNQPDRPILLQFCRHSSWHKNLQEQDPGSGNSLDFYLDKSCVVGLYSILDKTIFIEKPDDKYFDHYLGELHEFDDHDLSPLKFYILRCSTLMSSGKWNDFLRSAVGIKSMPSRISLRVPEAGVAFSVHFTQQLTRELEQMEIQERSILKIACLPRGYRVFQVPIIRYLLATVYDKLKDAGFTDVIKGWEKANVVMGVNFRHYDLINWCPGHQSHSLRSTCSLFQPHLLEFRPYAYCGRKLKLASGATVLEQTPIEGFLLRLTNQEGSDKDFLGNPHLIPTYFFTSENLLFSMAFAESTPPVPLELLISASDSLDPTALGDRLNEIPQVFEQNPYPLDLNSHIEWLNGTLDQEKFILNDFFAFKCFNRRIIQIIKSEAVIDLTEIEDIYQGSVTEVNSHELTYKSYALARSTFWRNEISVPETAKSIIILRHKNGLFLKLLAPNAAIGKEWVVRLKQLVFYWKEKQISELHKLWSTKMRNITNLQIEEEQEANVNGRTPKWVTDRGVTDPTIYNVNALAVLRPMLHKGILYQKPKKHAVFSKYMVVLVPGFLILYHSYRRSKTGFAKNSFDHRHYMTVPIEHCYVYSGTLTELDLLDRSRDFDELNPGTHALPRIYSDGWASSETETSRCFTLWFGKKRFLSRSSKNLQSEKDYQNEIPKMSDLKSEPQGGNTHNPNMLKMIRQLGVSGRSMVFMARSRQERDLWVLSLFYEMERLKNFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2SZ89</accession>
    <name>A0A8J2SZ89_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_11166g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_11166g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2SZ89"/>
    <dbReference type="OrthoDB" id="4953at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000015">
        <property type="entry name" value="Putative zinc metalloprotease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000031">
        <property type="entry name" value="Putative zinc metalloprotease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016:SF16">
        <property type="entry name" value="METALLOPROTEASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G07610)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016">
        <property type="entry name" value="PRESEQUENCE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="1">
        <location>
            <begin position="55"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="2">
        <location>
            <begin position="194"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1028" mass="116611" checksum="B93A731E2B4DF609" modified="2022-05-25" version="1">MGFNKLISVQLDYAPQYHLTKYVSTRTRLQLVHINHKSSPLVQGYFAVATECATDSGAPHTLEHLVFMGSQKYPYKGLLDTAGNLCMSSTNAWTATDQTVYTLTTAGWQGFKKLLPAYLDHILHPTLSDEACLTEVHYIDPEDMTDKGVVYSEMEAIETQSWFVTMLEKQKLLFPQGSGYRSETGGLTTHLRHLSNEDIRKFHKNSYSPENLCIIISGNLPEEELLSIVQDWDNELPTYDKTFYQRPFLDTESAHIPARLSKSVESTVEFPESDESQGELLFAWIGEPYHSYRRDLAVSMLMDYFTETALAPFTKQLVEVEDPFANSVDYWTDDFVRTIINLNVHGVPTEKLQLTKSKTLEVLSTHRVDLGRIRQVIDNAKWDYVLKIEKNGDTILSQAAITDFIYGHLDGSSLESTLKDLTDFDSLLEWSQEMWQDLLQETFVTNKPVIVVGKPSVNMNEQLEMDGAHLIKQREKEYGFQGKAKLREELKSAVLHNDKPIPVSLLQNFTLEDPSKTVDFISTRGITTIDGCEFNDHNDELTSRVLGAKPENFPFFLHLEHYPTQFVELHALLNSTSIKDASLLPFYHVLDEIFSMPMKLGGGKLIPYEDVISKLQSETIDAEVSLGLQATAPDLINFRIRCKAEDYAKAVEWIKHCLFDMVFDESRVRVLLDNYLTSIVEVKREGDLMLESLTGTHLYTERSVKKSVDPLFVEDKLERILEEIDQGKYEERILPKLELIRSQLRTTFSKHHLLILADVTKIGPDIYKPWESLAQALGEIPEGYEAKIPPSPRLLDAASPLCKAPGNKAFIITTPASESSYMNIITSIPFNLDYRHPDYAAVSLAAEYLQSVEGPFWKGIRGTGLAYGASMMKMTEVNALGFNIYRGADIIKCYQVAKQIVDQHASGEIDINPQQLEGAISMIINSIASVEKSYVSAAVSKYIDNFILQRGPNFNEYYLDKLSKVTIENVQIVLKKYFVNLFDCSKSVAFISCHPSKLESFQEFLEKEGFVVEVQELEEDEEDEEESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2SZC0</accession>
    <name>A0A8J2SZC0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BN860_11496g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_11496g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2SZC0"/>
    <dbReference type="OrthoDB" id="2019572at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035317572" evidence="3">
        <location>
            <begin position="21"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="420"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="4">
        <location>
            <begin position="21"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="471"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="544"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF87658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="610" mass="62647" checksum="A227EC12EE3A8C11" modified="2022-05-25" version="1">MGSRRHSLLALSIFCCLAWADYVHKGCFEASGLNLKSLGQYTWQSVSYCEQHCDGSAIVALKNGGECLCGDSVDMLSSAQSGTCDVKCFGWPYATCGGKDSVDVYVDSSYSSISHSHASGSSSHVTLTSTTTSSQASSGSTLSTKGSPISSSSAESSSSSSFVPSSSITSTSSSSSASETVTSSAKSSSSSASSSVTSGSSTSASSSPIPSSSVISSSSYASSSAATTSSSISSQSSSTQPSSSLSQSSSVISSSSTSSSSMPSSGSSSAQSSTAMSSSSSTQSSSAMSSSSTEQESSSSFSLNSISSTSSTVLSQTSTYDSSFAVSHYSSTPTTSQAAAAAVVASNPATTTSDSSSISLTTSIGYITKVIPTYVTTGDKGREKTIYVTTTSVQVSSLAAVSNAYGKSANKKGSGMSGGAIAGVVVGVVVGVILIIACVLFFLWRRHLSHQEPDIEETKHYQPYSFGDADANPMAYPPSRNTSNWRKGSTTRSSSYGTVPTGFLSASNSGKGSLSSLLHSEDAGNNYSRQNLPSTVFEEPPSIYNGNQRFSTGSLPDMMQERSLRVANPDDEANRLSQRRFDDEDEDDEDNIFSAGSSGGSYIGQEPKYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2SZQ5</accession>
    <name>A0A8J2SZQ5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_12618g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_12618g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2SZQ5"/>
    <dbReference type="OrthoDB" id="4032719at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="37"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="674" mass="78217" checksum="C1C56EEA14938569" modified="2022-05-25" version="1">MRRLQLFGRSKSHASRRGSPEKRPLVPNAKTKENTSSLGFLEFPPDVLCRILNQLDNNTLLCLCQVHTRLYKIVSNQYLYKHVKLEDKLALLKFNAMIHTEFRTAKVVCARCKPSESQNARFLVQTIEFKNPQCHDSLLKYSKFHQNDSSLIGGTYLYDSTHDIDSDEKIAQYIRYTYIELMLDIIDYLPNLTRVTLSDVQPGFKIPFWYSAFNDGTRDFFKKILKGQQSINSNDLRTFEVSPRFASDYESKFYSLPRIKVLEIKAAKDKRDRQQVALRPNLLCCFGVIDHLILESVIIDTDSLDTPLEFVAFHLRLDDSGVYDLHSPVHSLTLQSCQVIPGNGILRLVHDYFKCVRSLQLLDLTSKYDMLLCSCFNSLTDLTIDCNSKCFTNERLVSDSYYYQEDSSQNYDGNDCDSLTETLCDVPEDHTLSSPPPTTPIVMSLNLNYLSRNPGTNRARNKKQIALITKSQGEFFRQFRIPEFHYFYHYYKQLWDRLPRKNINVSIINIPFTNVYPLSPQLYCEKIINASNDDQQTLIGATQTDPSPLKNYYWDEPVRRCLMANIDPLLSSGQYGDLTDENILACTREEILNDYNNPKIFSDIPNVNLWCFLKSLSKFKSVKIQMFRQWLFCTPRSRYDWELLLRPVLNVDVPIEVRDKDGFVLYSYGSVKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T039</accession>
    <name>A0A8J2T039_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zinc transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_13740g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Functions as a zinc transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T039"/>
    <dbReference type="OrthoDB" id="78669at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904257">
        <property type="term" value="P:zinc ion import into Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000014">
        <property type="entry name" value="Cation efflux protein/ zinc transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045316">
        <property type="entry name" value="Msc2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45755:SF4">
        <property type="entry name" value="ZINC TRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="453"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="491"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="519"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="10">
        <location>
            <begin position="353"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="300"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="586"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="591"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU369017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF87756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="683" mass="73598" checksum="AAE83DB8B8306C14" modified="2022-05-25" version="1">MPLQQQLAMVPLLLSYPTVILSSDLILPAEVGVPRLVILPVFSATTFAAVACALHPKLSISAAYPVLGLGFLLFELSLLLGPVQAAALFILASKLVFAQHLEGRKSRLSVVCKQLWPLPFFSVAIALARGRTAGALVAPVIAYSMLIAAAFVLYPQAAALLKLPEGSQHETGKEQKQEKSARHGPGLRTALPLAALVALVLLYLVSALQPLMVFVFIGCLAIFFLSLSDVTSDQGPELVLQKWMVCVVGGLAAVLQYLSFNVVSASWAGDLMTGILVVVCEFISQSVEGVTGCTIIHEPESDHSHSHAQSHSHSHSDSHSHPHSNSKSIAHPNLHEESVLSQVARDPDTRSIFSFLLLNTTFMLVQLLYSFRSKSLGLLSDSLHMALDCTSLLLGLIAGVLAKRPPNDKYPFGLGHLETLAGFTNGVLLLGIVCGIFVEAVSRLFHPTALQETSELLVVATLGLIVNLFGLLAFDHGGHAEDSSNENMRGIFLHILADTLGSVGVIVSTILIKFTNLNIFDPIASIFIGSLILLSAIPLLKSTTYTMLLRLDDRRHNLIKSALNQISTTPGITGYTTPRFWASSSEGSAHSHSHSHSHSHSHSHSHGECDDLHSSKTPTALVGYIHVQHVEGENSTIIKKRVENIFESVGIRAWIQVEPNESPCWCRTPSLAQSVTSQGKMNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T1I3</accession>
    <name>A0A8J2T1I3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">BN860_18756g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_18756g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T1I3"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF87974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="879" mass="103159" checksum="EC5DD2D9C9F6B796" modified="2022-05-25" version="1">MLRIPARIRNVARIQLRSYRRKKPLTGTHISALQNNGLLQDAGEIKLEARDPREIETNLKTLGFEELNSTNYIEDLLLQYLKHTNSGFKPSVKNLLNLQRSLKEGKLDDTRRLKILFDYLLKESEIEIKMLNVMGPEKLREAIISKRKGASKEELESPEQIEDAIFSDLFHSAGQNSESYLNHVDLTYQILTDMNDRKNKGLEILSLEQLVQVFELSKLIPIEGRRKRGLFLAGNLIYSLGTVRMDPVNESFYIDSLVNYGFYKKAYDLFESNRAKVNERWWYELGMMVALRANYLQKFDKLMVASDLRFGEYPYVSLKVLKLAVKRKLLIKDIRSANKLTGRFLEIVAGYGCKRYDQQENDDKHMINFTSEEQADEFLNEKTAPTDYDFITLIDYNLFRKNIPMAMRLVAKYLQTEGITDQNYRFLIVQLKFNLLQNFEHLKNSLGPHMNPESAHQRLDELEKSFNDVRKEHNLDSSFSQELLFDNVASLASHPELIEIIETFLTRKVTTDDEKRQPSKKFQGLLKVLLATGKEEQAFFLLSKMEEALRKSQKSPELLGNQFYPEVQAHHYAVFVEYFSLLFVKVPKLVSNFERRITHLVDRMENLDVSYNSVLLTKLLIFYKNTNNFNKCFEIINQVMEEKFKKHMLSETDRTRLYNRRDVTRPLYLEIWRVYGKYYKIFHKELERVDKKSNYYGWKTQVERIIQRTKVHPDFPIRIVFCSMVHYDNVLPDSKMYHIILTTVMRSRDWAAIPGVLTTMAELHGLPLEDQFFRYVMRGLEKELIVVLTKKIKAQNSNMEESKIQALERVKELKLRGLLLVPKSGTKYDVDDLISQVLLLLRIKDSADQSFSEVTEAFKELKVEKKNFTKLVDHLNEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T1L5</accession>
    <name>A0A8J2T1L5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BN860_08416g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_08416g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T1L5"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07920">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000237">
        <property type="entry name" value="Pumilio homolog 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF13">
        <property type="entry name" value="PUMILIO HOMOLOGY DOMAIN FAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="4">
        <location>
            <begin position="527"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="548"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="584"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="620"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="657"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="728"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="769"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="97"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="153"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="246"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="480"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="107"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="166"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="284"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="301"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="480"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="491"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF87527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="872" mass="94071" checksum="739590D3E315C9CA" modified="2022-05-25" version="1">MSTGTTDKKAPDFPGVDPLVSESLDSALGQLHLEELDQNTQQPDQNTDSASTSVQLEQNVSDAMMPPSHMFPPHPQMIGMGFIPFSQMMAIPHHTGFFPPSDFNKDSSPAGPAPGTMPGTSINTSAPPVATVSQGTVGPVLFNNSNDASMFATPNGLSPGFTMGNNGNGNGGGSGDGRGNDDDNQNTNARSDVNAPPQIDPLWNGAPLGVAIDDSSALEDGLPQKTVGGNNITFRRQTFHALSPSELMHSTGNSAGLSAAEPMAAGSQKNASVAAPTAARRQSVHMDKMFSSKHPEENVTDTDSNNPNENDSSKNSKNFAAAYPYGGPLLQPNPILSGHHPPASAHAYGIPSPFPAGAYGFTSPFQSFSPVLGTANTSLQPNSSMTMAHSPLQVAPVPAGDPTVDPLMVAEPTDKSSNQFIAGDPASGTMQHPQAMQMGLHQQGGGTPPPWIYGGHPFGMVPHPHAVPQKPPHVMGPPVTNSSSHNVNLQGRRGNTNNRAGRSGHPGNGRGKHNKNYSHHYYGGTHSHENHQRKLEENSRYANATLDQFIGNIYSLCKDQHGCRFLQMQLDMLGPDAADAVFEETREHTVELMTDSFGNYLVQKLLEKVTIDQRRILARIAAPHFVRIASNPHGTRALQKLVECVNTDEEARIIVDSLNGSIVELSKDLNGNHIVQKCLQKLQPKDVQFIFDAACKNCTEIATHRHGCCVLQRCLDHGTKSQCEALCNILLKHVDHLTLDPFGNYVVQYIITKETEQNFYDYTYKIVHLLKPKVIELSLHKFGSNVIEKIIRTPVVSETMILEILKNRGEPDVQALLNDGYGNYVLQTALDVSHGSNASLYARLAEIVRPLMVGPIKNTPHGRRIMGILQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T1P5</accession>
    <name>A0A8J2T1P5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_10968g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_10968g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS52 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T1P5"/>
    <dbReference type="OrthoDB" id="19482at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007258">
        <property type="entry name" value="Vps52"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048361">
        <property type="entry name" value="Vps52_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048319">
        <property type="entry name" value="Vps52_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14190">
        <property type="entry name" value="SUPPRESSOR OF ACTIN MUTATIONS 2/VACUOLAR PROTEIN SORTING 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14190:SF7">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 52 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20655">
        <property type="entry name" value="Vps52_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04129">
        <property type="entry name" value="Vps52_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vps52 coiled-coil" evidence="6">
        <location>
            <begin position="54"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Vps52 C-terminal" evidence="7">
        <location>
            <begin position="238"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="588" mass="68328" checksum="96A3E51643D20C03" modified="2022-05-25" version="1">MEVLSGILGTDAINLSTQDSSNDVNHLQIYLEDCKDSKLQMNNNLWNELQQLQEKQTQIQLTLKTAVPPLRDYMERFNTQLAEFTNDLGFIRNKSSELKNMLEYNSTKLSKVSPLVNDLIIPPSVINNILNSKINAAWQENIAFIRDKQEIYAKYKNSNTTVPKDFQLLCQVLQYLRTVILERSRKFIIRSIKKLRSQQPTASQRIQERLLQVKDIFPFIVENNYSLALELRQAYAYTMRWYYREYFARYIRSLTILHFRNIDSQYSLSNSVSNVNASKPASAMFSNYLSHAYIKGGPVTAEAVQEYFQISRRLSILTQEDNIVMVSQIAENNTMSNYIEIGFKNLNLALLDNCSVELQFLKGFFKISDNEDELRGMLEQIFQHTFTKSAEYTRNLIQSTYDIFGVLMCIRISQQLQFESRKRETAVMDEYLNSTLILLWPKFQQLVDFQCESLHKIPISTMLTRSLDSSSQRDLLTTPLELTIQFSKFLTSLLTLAGTHEAVIDERSEPLYNSILRIRNDFETVMTKCSRKTDSPDRMLATNYMYLYNTLEHQNLTLEDQGVPFIVKETESHFKALVEALSKSHDKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T243</accession>
    <name>A0A8J2T243_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BN860_13586g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_13586g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T243"/>
    <dbReference type="OrthoDB" id="341421at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19177">
        <property type="entry name" value="SET_SETD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016852">
        <property type="entry name" value="SET_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044429">
        <property type="entry name" value="SETD4_SET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF47">
        <property type="entry name" value="ACTIN-HISTIDINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027158">
        <property type="entry name" value="Lys_MTase_YDR198C_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF87749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="454" mass="53175" checksum="6B344AA17C37F95F" modified="2022-05-25" version="1">MESKITSLVHWLNESDQFKLAPHLGVNESDESGRGIVLIDGALKKNEVIVSVPGTYQLNFHTVLHHISKFNRTLNIPRVTTSHEEIDEQEMSDPRYKAYNVLNQNFLLNLSSFQLLALYILAEWVLLPFWSHGRIKSFWEPFFAVWPTQEELKSFPAIWSCSKKSEYKHLLELLPSASEKHMIKLSKLMENDWAIISPILTEWDNLFRANAELPKNDELFEEFLHIYCIINSRCLYKEVPLKKHDLESQFTMVPFVDFLNHTQEVDVHCYPFKHSLQKDLYSLGPFTIKCGYHMYKLPGEEILLNYGAHSNDFLINEYGFVVKQNNWDSLDISKEVCAMIIDDAMIQFLKNNDYWEDYTINMTGISYRVVVAMSLAVTGDYKKTEKLLLGLISEDYFFPKIKDSLCVILLHLRKQFVQTIQFLERERDICSQNLLTIFQQYVEIIDRNLSRISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T258</accession>
    <name>A0A8J2T258_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cell division control protein 42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_13960g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T258"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01874">
        <property type="entry name" value="Cdc42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000236">
        <property type="entry name" value="Cell division control protein 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037874">
        <property type="entry name" value="Cdc42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF87764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="191" mass="21480" checksum="18031CD3DBCC3B37" modified="2022-05-25" version="1">MQTLKCVVVGDGAVGKTCLLISYTTNQFPADYVPTVFDNYAVTVMIGDEPYTLGLFDTAGQEDYDRLRPLSYPSTDVFLVCFSVISPPSFENVKEKWFPEVHHHCPGVPCLIVGTQIDLRDDKVIIEKLQRQRLRPISQEQGERLSRELRAVKYVECSALTQRGLKNVFDEAIVAALEPPVIKKSKKCTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2Y5</accession>
    <name>A0A8J2T2Y5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BN860_02080g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_02080g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10051416at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006113">
        <property type="term" value="P:fermentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029636">
        <property type="entry name" value="Csf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056779">
        <property type="entry name" value="Csf1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048636">
        <property type="entry name" value="Csf1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32085">
        <property type="entry name" value="PROTEIN CSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32085:SF3">
        <property type="entry name" value="PROTEIN CSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25038">
        <property type="entry name" value="Csf1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21678">
        <property type="entry name" value="Csf1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Csf1 N-terminal" evidence="3">
        <location>
            <begin position="34"/>
            <end position="1633"/>
        </location>
    </feature>
    <feature type="domain" description="Csf1 C-terminal region" evidence="4">
        <location>
            <begin position="1747"/>
            <end position="2984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="839"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2515"/>
            <end position="2536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2898"/>
            <end position="2917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2524"/>
            <end position="2534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2904"/>
            <end position="2917"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF87246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2985" mass="341543" checksum="272600D063952F10" modified="2022-05-25" version="1">MVSHTGFQKVSLSKTENFSWVFLVDWILTCIVTLTVIFYFGRIIGYLLGFIFEWVLWRRYKIKVNIESLRIAPLSGSIVFRNVCIIHRDYTISCMEGSINWRYWLWNVRRSQYEESLIEKEELEGGDEPKDNRRHPCRFQVECEGLEIFVYNKTAAYENIIHMFSKEERAQFEKFVDDQTLTELLSKTTEDHTDKVLTESTPSSITTQTVHNDSDNSNTNNSIRTTDNDDTEGADKKEAFDTTTNDRVFDQQYSKQKTMFMRFLPMQILIHRFSIVLGNRFTSSILIGSSQNAKGMIDLCQPREKLDIFKIKLQNELLDVDVSVKQNIGFDDDISTSFRLTRGRLSKLWKTFARVLHLPIADSRMKQTPEDASFHQSWKGLALYRQSLEDDELENIEFDFANHEYAKVSNILKSTRVIYTYEYDVPGLVPHGAQETLQGPEVGNSGAPPELKVDIQIYGATICYGPWAERQVSHLRRMLSPVVSRTPKPIKSLLPGSRRIYTVFRTSITIMEECTWRIPTREKSKDQDFLKHYIDTNEEFRPFGWIDLKFFKDSYGILDTVVCSTEEGSPNDIIFHFSEPEIRSSVNHELLLRSKSFDFEGDLGFPLGWNEEATWEINLSSAQLEIFLLREHITLLSDVFSDFASGDPTPYELFRPFLYKVNWNLNGYSIYLNVNDHNIVNNPLDFNENCYLSLHGDLLSFKVEIPHKTITHKSTDIVYCISTPMFRLLLNTPPWNTLNEFMKNKEVGRAYEFSVNGLYTIFSDLDVDHVDTVSVECSSTGTTLHCYGFVIRYLMNVKMNYFGDFSHFMTSEEYTSAIQKEEAKSGHFGDENSMFDINSGEGSDAEKNIPVGKQDANETPIPKRSNLKRTENESDIWFIFSVWEGALILPETIYNSDPCTALHFGELMVDLRSTNYYMDLLATMNNTQIKRYVSRPPHELFECVRRDNGRQEKSHGSLTTLTIHGHRMYGLPPIEPTYFCQWGVDLDHLEVDSDMNFAKGFFTCFSKIGFGFKNMENTLLYEIKTLYDMTSITVRAKELKITVNDEDSNSKIVLDAKKITFTSIDFENEAYSQRIDLKIPAINLMVLKMGRNNQGTNVLFELSTQLNFTNFIQRKNFADHRYKQREYIALHDSPFYRCPFILPCFFQESVLYTELLGSISPSSTLPPLPLPVVPKTVDYIIEDLLGNYASFVDHDYSRSPGTSHNTSGSSSSSDEVSSHTSSKFFSPHSSLNSRDRYNNYVFDIEFITVTAAPSVSDVVSSYLEKFYKEGTIDIIDENEVAVVKKLGSLQYGASFVTTIKLHVKSCNISWDYRSIKGIDISLDNLDFDLGERTIENEDEKVTMERTSLVTFHSLRANLSDTFSCEQDGKTPALAFTLEGFEVWSSTVDENVNSLNVASVDMTVDESQLEKVSMYLVEQGKKVLKVVSSAKRLQNLKEKFQKDLICDLTSASEYYQISHDPYVITKPAFIMRLSKGHVRENRSWRIITRLRHILTYLPKDWRTAEHFVTVKDSENPNVPQNDKDIFVSVFSNWRNWDLSDVTRSYLYRKIFLPGNTNSFSKELRVVLKLHFQSIFFTMLNTDNQAPHNVVITRANAITDILPPSVNTNAERLFKQEKIINFIANVGAIKGEISDQLLKLRSYLPNFNERPSENSFGSNGKVKNLKFYLTLIFERSDLQLILGSARLTNRIMNGRFSMLLENSKEYATPALSAVFFAQRFEVWLKHSTDVLAEVLLRQFCLAATVLFDPNMPTISINGQSASSLFSAMPSTAVMADSVEYIKESIGKLATLFNSQKEKAPTVSKAPSRPTMSLEVRLKFADISSEVTFLSPLFLSNDIKEFQIYARGLENIEVIVTIIELNIYLKSQQSAEQYCRISMTDLQLRSELSEDNSPVVDTQMSASLIKLSFAEPRRILHNLLRDEKILIESVPRMESLLHSIQSKGDSKNVQPDVSQEKSFFEKFRWAVDANLKYFGILIPMSSICYAFELHSLVTSLTNAGEQYVDHDRQFSGQLTIESCLFLVKESSLPASLSKVLDFSIRFLTSQKAPGSLQSYQIESSYFRVCLFPEMLVNLMWSIHHLQSLSHHFKKHHVPWFPHSRKQPVQHSESSLDFRSIHILSYGFCVGWLFQLGDNVEPGLILGYDRLFSAYEKNFGKLTLIDGYLSVANGSTSDSFYSQKSEKEEYNRTHLSNMQISYWLKGSDLLKDLFVRFHGEAIDVRFMSTSFRLVELTVQSICDFEEMKRARIRQAESGQKSKMNVEAASNSVAPFLAHIRTINSIFNFGGGVFKVYSPNDIGEKSEPMLEIKSPGVQLAINYEFNEAKKQPHWVRILTTVEPTHNTLFAKCAPLLTDFAENVKSMVRRFSSDDVPQSPKPNSEGINYKSLIKPYDLAFEITSGKQKLSFSCEPKAKVQVDTGFDSFLLKITTNDLDEVEPFSVSFSMDRIDASVRHIFSRDTSASFKLEYIDIVLMFTHPDVYGVALISDINVYCNMKQLQNLKLFLDIWKLSNSNLFKPKENTEDKKELKSSSTSFSASPSNGARRVLPWCFSIILTNLGGVIDLGPSLGVLTLGLERFWLAADHYREQRRVLHMFIDGITVNSEGRLSGMFELDNACCVYEMTWPDDQLLRGVPLMSFSLDVNKIVIKSAFDYHMFLIGTLQRVRIYLHNESDHYALLPDLLQVSLECEHIVLCATALTAANILDIYNTIMRMQQDNKISYIETLRESDSSASQTSLAYDDILKSLNLLRTDLSVTINEFKIHISPMSLFDLEVLVISFKDVIAHSETQSGEKLKTELQLQISDVKASLSSSKEELDEERVSKIPVDDYMLYASKISGGTIINIPKLLVSMTTWQKQNSNVLEYLFTCKFFDKISVKWNLGPVNFIKEMWTTHVKSLAVRHNQIGVPGREPSDKDAAQDQETESKFKYVPLDEPYIEMPQIKDLGDATPPMEWFGVHRKNLPAATHQTAVVLIQKLVHTAEKEYAKIQQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2Z1</accession>
    <name>A0A8J2T2Z1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_18932g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2Z1"/>
    <dbReference type="OrthoDB" id="274922at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000002">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038:SF16">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="15">
        <location>
            <begin position="22"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF87982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="95" mass="10427" checksum="54FB6D0D8748989C" modified="2022-05-25" version="1">MSFLGFGGGGNQPQVSSQQKVQAAEAELDLVTDMFNKLVDNCHKKCINTSYTEGDLSKNESTCLDRCVAKYFEANVKVGENMQQIGQNFQTAGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T376</accession>
    <name>A0A8J2T376_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Adenylate cyclase</fullName>
            <ecNumber evidence="5">4.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">ATP pyrophosphate-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Adenylyl cyclase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">BN860_08482g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays essential roles in regulation of cellular metabolism by catalyzing the synthesis of a second messenger, cAMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the adenylyl cyclase class-3 family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="5"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2021138at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01775">
        <property type="entry name" value="RA_PHLPP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000084">
        <property type="entry name" value="Adenylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013716">
        <property type="entry name" value="Adenylate_cyclase_G-a-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048580">
        <property type="entry name" value="CYAA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055071">
        <property type="entry name" value="RA_PHLPP-like"/>
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    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08509">
        <property type="entry name" value="Ad_cyc_g-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21187">
        <property type="entry name" value="CYAA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RA_3"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="7"/>
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        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="9"/>
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    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="12"/>
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    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="7"/>
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        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="809"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="domain" description="PPM-type phosphatase" evidence="20">
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            <begin position="1490"/>
            <end position="1756"/>
        </location>
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            <begin position="1800"/>
            <end position="1937"/>
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    <feature type="region of interest" description="Disordered" evidence="17">
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            <end position="311"/>
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    <feature type="region of interest" description="Disordered" evidence="17">
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            <begin position="541"/>
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    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="729"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
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        <location>
            <begin position="41"/>
            <end position="58"/>
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            <begin position="159"/>
            <end position="176"/>
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            <begin position="180"/>
            <end position="194"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="17">
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            <begin position="237"/>
            <end position="251"/>
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        </source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS51746"/>
        </source>
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            <dbReference type="EMBL" id="CDF88362.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
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            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2152" mass="241205" checksum="431690566FC8653A" modified="2022-05-25" version="1">MFRQGDKHQDGSRKQHRADNNLARITTGMSVGSQGSKGSKTSKDSIRSTREKDSRTMSDGEGSDGGGSLVTRGREAVSAPLGKSISTGEVGGVGGNHADAHSTSNYQQQSQLQSQDRHNHRLRDSKIRKGPILLKKTLSSLSLGSAASDDGDNQHSHQRQQQRQQHQQHQQHQQQHVPMETQQKLPTTASSATSRRYPHGRPLPQNGGAMVAPPRKQSLAGSFFKKLSGSGNPDGPSGTGTATGAGNGGAYGEEQQHSRNGGPRSGHHGGLHSMYSATPSGGSRRSSKDYHPSGGSGGGGSGGGSSGHLMETVPSSLMRKMSTLVHGFGHDLETSSHSAKRPFYHEKKHDTDNISLPSSRPRLSASSSRRNSATSTITNSTLAHMQAQAQSQGRSQSQGQSQLQSQEQGQEQLHSGPETSLPEQLHERHQAQEDEGKQEWQQVQQQLGESEGQRPEPPQKEKQGSRSCADRQGSVFQLDTNLENVSDITKAPDLSRRPSAIYDGAHPEDKRVSVSGPSASITHVNSRNDLSALPSSLTVAASSPNAKQMWVAPESWDVESDVDVRRAKKHNRNLQYQQEQHVSLNKDPHMDASSGRPNNRSTKSTGPGGKESMSSSLQSSEQSSSMESSESASSDLDYSSGMESYSERGRGDIKAIENNEQHPPEGSQQQEQRHIQQEKQLDHQEQILRQQLKYDEVNQDQLENLDDKAKEEALQQHQEREKQLLYLTQQQKKRKRQRQQKEEQQSRQNVLHSQQDAPTSPSKSLAKALGGSTSDQETMKLSFDDEKHDRVEYELERYYKDFSDLDPKRHYAIRIFNTDDTFTTLSCTPATTVRDILPSLKRKFNVTSQGSYQISLKVGKLSKILRPLSKPIIIERKLLLLNGYKKSDPLHIMGIEDLSFVFKFLFHPVAPSHLTPEKEQRLLRSDFVHVDLRGMDLTTPPIIFYQHTSEIESLDVSNNANIFLPVEFIESAVKLSSLRMVNIRASKFPANICDVENLISLELQRNFIRKVPHAVSQLTNLTILNLQCNELDKLPRGFNQLKNLQLLDLSSNRFVHYPEVVNECTNLLQINLSYNKIHTLPASTNKLFKLAKMNLSHNKLTSIADLSEMKNLRTLNLQHNRVARIKTGARNLQNLSLADNRISNFDDILPKLKILELQENPITSLSYKGFFPMNMTNLSLSKAKLSNLPGELFFELSCLEKLDLSENNLTKLPDEFCLLNKLVHLSAARNKLQKLPSNFSKLRSLKSLDLHSNNIRDFVCNAENLEITYLNISSNMFGNSLLTGQFCQDISPDSKLANSLEYMVAADNQFNDQMWPLFNCFTKLKVLNLSYNNFQDISDLKLENLTNLYFSGNKLATLSGDVVLKWKNLETLMLNGNYLLSLPSELSQLEKLAVFDVGSNQLKYNISNYYYDWNWRKNKQLKYLNFSGNRRFEIRSSVSPDINADLSDLTVLPHLKVLGLMDVTLNTSKVPDENINFRLRTSGSVINGMRYGVADTLGRRDYVSSRDVTFERFGGKDDECLLCLHDGKNHYADYGHNIARLVRDIYDKILLRQLERHGDSDLGIRKAMRFSFLQLNKEINGMLSCVDNGTAVANLTSADLLSGACSTAVYIRGNKVYTANIGDCMAILSKTNGDFQKLTSLHVPYKREEYERIRISGGYVNDDKLDGVVDVSRAVGFFDLLPHIHASPDISMVELTKADEMLVIATHKLWQYMDFETACDIARENNSEPMLAAEAMKDHAIAYGCTDNLTILCLSLYKNSDQQNRFTLNRNALMTRRSTFEDTTLRRLQPEIAPPTGNLAIVFTDIKNSTFLWEMFPNAMRTAIKTHNDIMRRLLRIYGGYEVKTEGDAFMVAFPTPISALVWCMSVQLKFLDAQWPEEITSIQDGCLVIDKQGNKIYQGLSVRMGVHWGCPVPELDLVTQRMDYLGPMVNKAARVEGVADGGQITLSSDFLSEFNKIMKYHQKVVKENLPLKEVYGEEFVGEVLEKEIAMLENIGWVFFDHGEQKLKGLETKELITIAYPKSLASRHNFITKDEETRMLNEGFICQLRCVSRRIESVLSAVSKGVVDEQRKPGKISAVDESVKNSVKFLNSEKDATAYFDDLVTRLESSIALLKLHQVVNSGFKVKGHDTFQSHGTGIFDILDAVLEKIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3B1</accession>
    <name>A0A8J2T3B1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ADIPOR-like receptor IZH1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_11232g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3B1"/>
    <dbReference type="OrthoDB" id="529367at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF95">
        <property type="entry name" value="ADIPOR-LIKE RECEPTOR IZH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="207"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="35693" checksum="EBBA73305E9F7DA9" modified="2022-05-25" version="1">MSSITEVKQRRTPVGNPSAEEDSQDSKLENKKLYDFHELPEWQQDNDKILTGYIRETRSWRKCLRALLIWNNESVNIYTHLVSSISYFLLMVGLTNVVAVPVFPTTTMMDFSVINFYLFGAFGCLMCSSCFHTFKQHSGPHCDAWSKVDYMGIIGLITCSIISLLYYGYFDSKEYFVLFTIVTVLLASACSVCVLHEKFNHKDFRTLRAAFFIAFGFSGIFPVATGVLKFGVAEVAERVQLRFVLWEAFFYISGAVIYGFRIPETVAPGKFDFLGHSHQIFHVLVVVASFLHLRAVMGSYVFKHTHLGGPGWLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3E5</accession>
    <name>A0A8J2T3E5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BN860_16028g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_16028g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3E5"/>
    <dbReference type="OrthoDB" id="2148490at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF65">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF87857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="299" mass="34208" checksum="3BA00C4A35AC0D8C" modified="2022-05-25" version="1">MFARPVVRLRHTACKRNFSLFQNVTDTLITLHDVSSLPWIVVIPLTTIGLRTCFTLPLSIWQRKRIVKQQELRKIVQASSPVTKLRLAAATNSPSRAIDEISSSGSVVKETQTKLQKLTPEQISLLSIKETRKRQKALFSRYHVQMWKNVLLPLVQIPLWVTVSMGLRNMTERRVLETNNWDILQHWLPPTLDLSLPWDAMPMIAPMILGTISLMNVEYNGKMMTSTSTSLVGVETASRDYSKMSQAMQGILNLSRLSCVFMMGVSSQASLLLSLYWISSQLYSLVQNIFLNWLWPYQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3Q0</accession>
    <name>A0A8J2T3Q0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Maintenance of telomere capping protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_15192g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in telomere capping.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the MTC6 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3Q0"/>
    <dbReference type="OrthoDB" id="5573651at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051008">
        <property type="entry name" value="Telomere_Capping_Maintenance"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057530">
        <property type="entry name" value="TIM-barrel_MTC6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35518:SF2">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35518">
        <property type="entry name" value="MAINTENANCE OF TELOMOERE CAPPING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25506">
        <property type="entry name" value="TIM-barrel_MTC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035262396" description="Maintenance of telomere capping protein 6" evidence="11">
        <location>
            <begin position="23"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="464"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="MTC6 partial TIM-barrel" evidence="12">
        <location>
            <begin position="29"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF87819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="513" mass="58407" checksum="385FFAFCBE1D6C9C" modified="2022-05-25" version="1">MNGILLALLIYVMVWRPRRCLCDEGEDLPTVASQVQYGVRSQRDLMGNVTIDQVPMVGVNVYDTLFKSGRNNESRALMSVMELMQHGVQSFVIDLEQRSNQLILRKSNVSFDKFLNVLQSYVNNSDNNLSANMMVLLLRISTEMVTKKTNQSSPAGSGYNSSSQSVNMTYLLDQNLGRQRIYTPDDLENDRELGQTYNTNGTSKVGWPTLSSFLYQTKRRVVLAELTETSNKAYMPYVFNGSSIMNFDLNNATLGLPQTINQIKALSEKSWRYLEASFTPADIKVYMDYGYNPIIANEYAADNFASISELLNTTLMWSWEPNEPYSRTTDRNTLKHNLVAYNCAVLHYTALNSSAAWIVDNCYTKHRGLCRNKGNGYLWRTTVSYDTYFEFSESKCPNGFEFSLPKTPLEQLAVSQYLANISSEDTNLWIDMNSVYVNDCWVTGGPHATCPYQRAVSKRNFVKMITPVTVCSFVILIIVCYLNFVRVPIHNNRKSWKRIVNEVSKWEVDGVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3R8</accession>
    <name>A0A8J2T3R8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Importin-95</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Karyopherin-95</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_07096g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the importin beta family. Importin beta-1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3R8"/>
    <dbReference type="OrthoDB" id="10263328at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000027">
        <property type="entry name" value="Importin subunit beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="12">
        <location>
            <begin position="24"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="11">
        <location>
            <begin position="411"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00079884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF88310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="860" mass="94445" checksum="8D469597F6C5C147" modified="2022-05-25" version="1">MSSELAQLLEETILTPDQSVRVANETQLKKLSNENFLQFAGLLSSVLVDEGARLEARILAALTLKNELVSKDSIKASQFAQRWITVVDPASKDQIKLNAVQALVTGEPRVANAAAQLIAAIANIELPRGEWGDLMKTMVDNTNPSQPENIKRASLLTLGYICESGDPQSQVLVSSSNSILIAIVQGAQSSEPSVPVRLTALNALADSLVFIKNNMEREGERNYLMQVVCEATQAPDSAIQTAAFGCLCKIMSLYYPFMKPYMEQALYALTIATMKSSDDWVASMAVEFWSTICEEEIDIAYELAQFPQSPLQSYNFALASLKEVVPNLLNLLTRQNEDPEDDDWNVSMSAGACLQLFAQNCGNHVLEPVLQFVEQNITSENWRQREASVMAFGSIMDGPDRTQRTYYIHQALPAILNLMTDPVVQVKETAAWCVGRVADLVIDSIDSQQHLPNVVHACLIGLQDHPKVATNCAWTVINLVEQLAEAKPSPIYNYYPALVDALIKAASRPDNEFNARASAFSALTTLVEYATDAVAETSASISTFVMDKLGQTMTVDETQLSMENRQSLQELQSSVLTVLAAVIRKSPASVESVSDMLMDLFLKLLDKKDSSYIEDDVFYTISALASSLGRGFEKYLENFSPYLVRALNQVDSQISITAVGFVADISNSLGEDFKKYVPAFMSVLGQMISAENAKKELKPAVLSVFGDIASNVGPDFTPYLNEVMALCVAAQNTKPENGTLEALDYQIKVLEAVLDAYVGAVAGLRSSPEALFPYVGTIFQFISLLAEDPQLYGEDSTARAAVGIVGDIAAMYPNGSIKQFYAQEWVTEFIKRTRSNQTFSQSTKDTARWAREQQKHQLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3U7</accession>
    <name>A0A8J2T3U7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD(+) diphosphatase</fullName>
            <ecNumber evidence="4">3.6.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_17040g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5'-end NAD(+)-phospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-adenosine-phospho-ribonucleoside in mRNA + beta-nicotinamide D-ribonucleotide + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60876"/>
            <dbReference type="Rhea" id="RHEA-COMP:15698"/>
            <dbReference type="Rhea" id="RHEA-COMP:15719"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:144029"/>
            <dbReference type="ChEBI" id="CHEBI:144051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:60877"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family. NudC subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.22" evidence="4"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3U7"/>
    <dbReference type="OrthoDB" id="10249612at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019677">
        <property type="term" value="P:NAD+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006742">
        <property type="term" value="P:NADP+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03429">
        <property type="entry name" value="NUDIX_NADH_pyrophosphatase_Nudt13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050241">
        <property type="entry name" value="NAD-cap_RNA_hydrolase_NudC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049734">
        <property type="entry name" value="NudC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904:SF6">
        <property type="entry name" value="NAD-CAPPED RNA HYDROLASE NUDT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904">
        <property type="entry name" value="NUDIX HYDROLASE, NUDC SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="10">
        <location>
            <begin position="218"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF87901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="379" mass="42353" checksum="FA389BBE4DD822C6" modified="2022-05-25" version="1">MSAIRSASTFFGCETLNRVSFLREDVEFVKSTLTHGSTTFVPFLGGEALSLNGSLYQIQLDDSPSLLQPLLHKLLPLVNTPAGRAHSSGVNLTFLGLKQGEEAREGVFVYRSQYKGTPYYGIDFRVDSETIIKPQDIVALTKMTPLNRQEVFEMNNEEASLYSHAKMYLDWLSKYNFCPGCGSPMYPLDAGTKLKCSNLNSQVNCQVRDARVTNVCFPRTDPVVIVAIASRDFSKICLARSKRKHTDTIMYSTIAGFMEPAETVENACRREIWEETGIRCDDVAMVTSQPWPYPVNLMIGCLGIVDANGINEVITLTHDEELLDAQWFSTEDVSKALDDYNGNGMVHFKDSIRLPGSTAVAFHLIKHVCDKYKRRQGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3Z9</accession>
    <name>A0A8J2T3Z9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_15566g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_15566g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3Z9"/>
    <dbReference type="OrthoDB" id="21418at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38645">
        <property type="entry name" value="CHROMOSOME 9, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38645:SF1">
        <property type="entry name" value="YALI0F12243P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF88665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="218" mass="24869" checksum="80E0EA7FDAEB5FC3" modified="2022-05-25" version="1">MERLNALGSSLPPEQPPTNQVIESLNSELSQEFKLAANAVTKLYRVANERNSLLKHQGYLHCLEDVLSLLEQNPEAPVDAIHLWCLKQRNDMLSHGSISQDGPNGSKFDFNFDNPNVASTNNTNVSVPKFRSSHPPLSVEHNLAHKAKKTRWTKRHSTWAKEDQSRDVAKEESIQRLQRESELDREQDKMDSMERMEPKDSVPKKKHRVSNKKNQLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T417</accession>
    <name>A0A8J2T417_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="7">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_13498g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="7">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="7"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T417"/>
    <dbReference type="OrthoDB" id="193716at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070013">
        <property type="term" value="C:intracellular organelle lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="110"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="325"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="598"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="615"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF87745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="624" mass="71222" checksum="AE95B2B6B51AAE4F" modified="2022-05-25" version="1">MFLRCGSAKIPLQLCARTRSLFITSVRFYNGRGGRYEGERKSPRSRSRFSDTPVSLKKAKLIQIPFEENAAEVTLDELKDEGLIGKDVHESITRLGFEGLTPVQQKSIGPILKNEQQDVIARAKTGTGKTFAFLIPMFQHLINTRMENPFAVKYVIVAPTRDLALQIQTETQRVQSMNPTLKKFNSLTLVGGTNFGTSINNLVRRRPQIIIATPGRLLDVLRDYNKYFTHIDVKVLDEADRLLEIGFQQDLERISETFNKLNDNSEDHIRTLLFSATLDTKVQDLSANIMGKSECLFLDTVDKNEPQAHEKIAQSVVITPNLAHSIHAMVDHIKREVQSNPSYKAILFTPTIKLTSFISETLHSLLGRSLPILEFHGKKTQMARTKMVHRFKRDHSGILVCTDVAARGLDFPDVGEVLQLGVPPQTPQYIHRIGRTARAGKEGKATLFICESETPFLQYLAKKERINIEDQTEFKASEESIEELAKKLYDDEKLIDAFISAIAYYRANGRVFGFNDNRILSQIAESYGTLLNDRDRKIPVSANLLEKLGLSRNRVVQDMLNVQRSYRKHHNEDTMQAKNDRSRDKRWYDEAPFQNFVKRGKTKNQNFTKKSYNKTRGDNPRFDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T428</accession>
    <name>A0A8J2T428_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BN860_09736g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_09736g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T428"/>
    <dbReference type="OrthoDB" id="5368598at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023041">
        <property type="entry name" value="Glucose_rcpt_Git3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022596">
        <property type="entry name" value="GPR1/2/3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF37">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR GPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11710">
        <property type="entry name" value="Git3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11970">
        <property type="entry name" value="GPR_Gpa2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="554"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="591"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose receptor Git3-like N-terminal" evidence="7">
        <location>
            <begin position="58"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="G protein-coupled receptor GPR1/2/3 C-terminal" evidence="8">
        <location>
            <begin position="545"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="341"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="386"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF87582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="919" mass="106325" checksum="829B625AB2A90A27" modified="2022-05-25" version="1">MVHFFSDDEYTHSRPPVPTNNPSNVPLAPQLLHRNTTAVNQVLGLPGMFSTFNKYQLKRLRIVAITSSSLSLLFGIIGVYCLINIDRRRKVFRHDLLFFLIVCGFIKALVLMIYPMVILIRDSVYFNPLFFNILGWFTAYTVEGSDLAIFFFAVHFALLIFRPSHKWRNKRTGNLEGGLYRVRALLWPLTGSVPLLLASLAFVNFTVINERELLEETTVVLYNQRYKADHYARLGGYKPYSAWCYLPPYPLWYKLVLSWAPRYFLIIFILTLYATIYVFVLKESRKIKRELGDFRQKKDKGNWRKTSSVSSSRYLRFPAESVMKSLRAIKYVIFFWSHSSTKRGGGETTNSREHGITLSVFDDIDRPQAEEGELNTRHHTSKKNDNSNQKKGVNVGSSLPRTNLNSHQTKNTNSIIQADQSSQHEPTYLGCDSVGDSYEVEERDSVEGDLYEALSTPLDVPVEATSSKSIPIGQRLALQEQGIDNRHHVSPTLSGPGISTMQTIFRPLTNISGTSQRSHLYQASKKEEVNNVQADFQRQTYAEMKRRRLQIQKNVTLIFIYPFSYIAIWIFPLVVDVTQYNYEITHGPVVWLAYIATIVQPLNCFVDTLIFLFRERPWKYSWQEVQTKELMDTYMLKGELGENEIMELCSSEWGRRGWYYRGRFERKDCWRHQPWKWKRAVWFIYRLFKGLMRNHYNFEDHCNDEEYWDRYYCANSEPGCTVGQKHFSSQKERQFSFPSDSTTLSDSAHNQIKQSYANKRPSTLEESQYRQVPLFWKIIHFFPMLEAIDLDELNRQLRLRSKDDEFVIPGLQAALQSKFEQPHEAKPQPSVFKPGYSISDKVDGSPWLARRDHQSTEISNASNSNHNNGSTLPVTVNMDFSNVVAGNAASSASKKLSIAATNQDDSMDMLSFLKGHTIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T464</accession>
    <name>A0A8J2T464_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BN860_13652g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_13652g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T464"/>
    <dbReference type="OrthoDB" id="10259687at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048026">
        <property type="term" value="P:positive regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000895">
        <property type="entry name" value="Hsh49p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052084">
        <property type="entry name" value="SF3B4_spliceosome_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030:SF3">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="10"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="106"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF88583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="210" mass="24059" checksum="A9632FBDEF1EB7E8" modified="2022-05-25" version="1">MNAAVNNPETTIYVGNIDPRVTKSQIYELFVQVSPISEIRYPKDKVLQMHQGYAFVECCTPEDAQYVIKLMNNTVSLYDRVLKVRRANAQSTNISNPGDISMWPLAKVFVKNLDDTVDPMQLSNLFSKLGPLARQPEIFYLSHNELRCAYIYFKDYAHADTAIATLNNQLVVNKRINLDYAFKENSQTNAKYGSEVDRLLNKEARNRNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T490</accession>
    <name>A0A8J2T490_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="13">BN860_09472g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_09472g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T490"/>
    <dbReference type="OrthoDB" id="5353557at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010960">
        <property type="term" value="P:magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000006">
        <property type="entry name" value="DUF21 and CBS domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045095">
        <property type="entry name" value="ACDP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064">
        <property type="entry name" value="METAL TRANSPORTER CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064:SF97">
        <property type="entry name" value="METAL TRANSPORTER CNNM-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5 7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035260677" evidence="10">
        <location>
            <begin position="24"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="12">
        <location>
            <begin position="46"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="310"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="432"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="618"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="432"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="511"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="549"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="639"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="671"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF87571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="681" mass="74551" checksum="A55EC7FE2BA57462" modified="2022-05-25" version="1">MLRSRHAALPAVLLASVIPRIHSLPLVYRGTSSGGQPTSPHSHQPEYKDRTTYMIISACLVVLGGVFAGLTLGLMGQDEVYLKVISTSGSPKERRLANKVLSLISRGKHWLLVTLLLSNVITNETLPIVLDRCLGGGWQAVASSTVLIVIFGEVIPQSVCVKYGLEIGAFFTPFVQVLMYVMLPVAYPIAVLLDNLLGEDHGTMYKKSGLKTLVTLHRTMGVERLTQDEVTIISAVLDLKEKQVQEIMTPIENVFTMSADRVLDEKTVGEIFDSGFSRIPIYLPNEPTNFIGMLLVRVLISYDPEDALPVSHFPLATLPETAPETSCLNILNYFQEGKSHMCIVSKDLGSATGAIGVLTLEDVIEELIGEEIVDESDVFVDIHQRTIREQPGPLSKRHITSYLHRLYTNSNKRPSSDIQDGESQALLNHSDSAANSVPLSPTASHREGAQHMRLSSPPPGSEHAHLVLPSNPASNPLDVKKPFVTIKRPNPTLAPRNSSLYGTVRKPAKPGTKENSSDSREEEQTPRNSQSLSERERINNHVEVANQAREFTQRTGTPVQVTTNLAPPKDDGNEHASNSTGNHEPENALLSSSYKSTKHGIVESIITVKGVPKTIIEPAQNWDQNPKAGDDLVIEDQPETNAKADSNGNFNLEDPKTSKPKNKQTNGKSRGGGKKKSHRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4B6</accession>
    <name>A0A8J2T4B6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">BN860_17436g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_17436g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4B6"/>
    <dbReference type="OrthoDB" id="4062049at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF87918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="329" mass="38091" checksum="069DF19D08E50981" modified="2022-05-25" version="1">MKFVTRPIQDKVSHLVVKFQQPQCGLKVSNYPVTASQKSVLEVPIERKPIDLGMNDLAKKNTFLGALEPYHNCDNDALASRVKGTCSVAQKYQNASHIFSKESLVYHCRYFKLNYYPPDHFLSVLRKSKPAINVRESGEAYFNNNFNLDTNFHGDMDLVQKRAAYLFRNSPSFLPKQYAVYRRRNRMFVRERMVEEWCRLKGSEAVNETIADLRSEHGTQYLDDAGRTKPGIAKDGLYVYQVLIFPDRQNHRELGEHIRKSVRRVAYLQWDEFLRSKGNDKSWVQLANDRVRLDVLNKQLAQSELPFIVTRASSSFERGNLDDTDGTNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4D6</accession>
    <name>A0A8J2T4D6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">BN860_16578g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_16578g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4D6"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="264"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="338"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="408"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="494"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="533"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="574"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="645"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="687"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="721"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF88707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="785" mass="87633" checksum="A8F6D333512A2121" modified="2022-05-25" version="1">MSLKSIPQVKEVEEPSMDSGGEEPEKSSNSLEPPLKDANDLEMNEVYCQNDDIDYRDGIGANPLTTSEVMDITLRDIDNVVNLEEGFEDLPACTLRMWILAMCLATVIAGVDTFFAFRFPAIAIGAIVAQLVAYPLGVLWARVVPKWRIPLGKVGSIELNPGKFNSKEHALIYLFTNTVISTGLLRNTQVEQVRYFDENVGIGRFILYDIVGFLIAWGLAGLSIPILVDREELIWPEVLSSCALMEALHAGTKSSFSDWKMSRFGFFVWAFVGSFVWYWVSDLILPFIADLGAFPSWCKPKNAVLGQVFGVKNGMGLLPISFDWSTISTLSNPLLTPVWAAGTIFASFVFWVWIVLPGLYYQDWWETAHLPLMSNSIFNKKGTTYTVQKVVTSDWKLDLEKYKAYSPVYLPIGFLLYLALGLGGFSSMMIMFFWKFKTEVWTPFKQHQSKPTAKYLPASTRVFQLIYLFSMAVGIGLGFAFVEGWHRDTQITSGGYIVSVVIGVVLFLPVALIESKSTFTLNMEGFFNIVSAFWFEGQPITSLYFLNFGYAIFQHAMHFTQGAKLGYYMKTPPRITVIVLFAAGIWSSLVTSSVTGFVLYHFPDVCTTDAKNNMTCKSQKTAFNTQIIWGLFGSHLFGGGGRYNFIMYFFLVGAGVSIVVIAMQLLRPKSQFWHRISPALLMSGAENIPTATGLHYGSWMVAVLLFNFIVHKRNSAWWRKYNLILASALDCGVAIAAIIVYFAVSYTGGSANYKWWGTTVVDAGCDDAGCPFKSKSTISLPKGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4K0</accession>
    <name>A0A8J2T4K0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BN860_16952g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_16952g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL12 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4K0"/>
    <dbReference type="OrthoDB" id="250175at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00387">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.10:FF:000001">
        <property type="entry name" value="50S ribosomal protein L7/L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.710">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00368">
        <property type="entry name" value="Ribosomal_bL12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000206">
        <property type="entry name" value="Ribosomal_bL12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013823">
        <property type="entry name" value="Ribosomal_bL12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_bL12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008932">
        <property type="entry name" value="Ribosomal_bL12_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036235">
        <property type="entry name" value="Ribosomal_bL12_oligo_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL12M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00542">
        <property type="entry name" value="Ribosomal_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16320">
        <property type="entry name" value="Ribosomal_L12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="ClpS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48300">
        <property type="entry name" value="Ribosomal protein L7/12, oligomerisation (N-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein bL12 oligomerization" evidence="5">
        <location>
            <begin position="43"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein bL12 C-terminal" evidence="4">
        <location>
            <begin position="109"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF87897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="176" mass="18573" checksum="E7EF5CFF354498FF" modified="2022-05-25" version="1">MLRLVAKRSAVATPSILRATPIFACRCNSTEAAQQGSQPVDPKIANIVNEISKLTLLETSTLINELKTQLKIPDIALPVAGAAAAPVSGAASPAEDAAQEEKPEEKTIFAVKLESFDAKSKPKIIKEIKGMLGLSLVEAKKFAEAAPKVLKDNVAKDDAEKIKSTLEGLGAKVSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4Q9</accession>
    <name>A0A8J2T4Q9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FACT complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_04214g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the FACT complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peptidase M24 family. SPT16 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4Q9"/>
    <dbReference type="OrthoDB" id="10251642at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000002">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.210:FF:000001">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000017">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000006">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000005">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.210">
        <property type="entry name" value="FACT complex subunit Spt16p/Cdc68p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029148">
        <property type="entry name" value="FACT-SPT16_Nlobe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056595">
        <property type="entry name" value="Fact-SPT16_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048969">
        <property type="entry name" value="FACT_SPT16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013953">
        <property type="entry name" value="FACT_SPT16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040258">
        <property type="entry name" value="Spt16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980">
        <property type="entry name" value="CDC68 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980:SF15">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14826">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24824">
        <property type="entry name" value="PH_SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08644">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21091">
        <property type="entry name" value="SPT16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01285">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01286">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FACT complex subunit SPT16 N-terminal lobe" evidence="12">
        <location>
            <begin position="6"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="FACT complex subunit SPT16 middle" evidence="13">
        <location>
            <begin position="547"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="14">
        <location>
            <begin position="825"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="448"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="764"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="947"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="474"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="948"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1012"/>
            <end position="1027"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF88184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1027" mass="117385" checksum="67B43A46E40B295B" modified="2022-05-25" version="1">MVELNIDFDVFKKRLLTLHAQYKSYENSPNSLLFVLGSSNTENPYQKTSILHNWLLSYEFPSTVIAFLPDKVVIITSAAKAKHLEKAVEMFAHEEVKLELWQRNSKEPEHNKKLFEDVIEAIQKAGKVVGAPEKNIYQGKFMLEWTPLWEEAVKKYELKVVDVSAGLSRTWEVKDMNEQALLSAAGKSSDKFMDYLADEMVRAVDEELRITNAKLSDKIENKIDDDNFLKKITPEVSKLCPKGHSFNIDLLDWTYSPIIQSGNKFDLRVSARSSNDQLVGNGCILASCGIRYNNYCSNVTRTFLIDPSEEMVRNYGFVLDLQKEIITNKLKVGSTPKAIYDAVVEFVQKVRPDLASNLTKNLGSIMGLEFRDSSFVLNSKNDTRKVQAGDCFNISFGFNNLKDAKTGKSYALQLSDTVLLPSEDSAEPQYLTNCARSTSQISFYFNNEEEENESKKSKKPAKPEPNSKILKTKLRGEARGDSQENQKEQIRKENQRKLHEKLQKNGLLRFSAADANGSGTEQRQYFKKYESYVRDSQIPTNVRDLRVHVDWKSQTIILPIYGRPVPFHINSYKNGSKNEEGEFTYLRLNFHSPGAGGISKKTEELPYEESPDNQFVRSITLRSKDGDRMSDVFKQITDLKKDSTKREQERKLLADVVRQDKLIENKFGRTKRLDQIFVRPSPDAKRVPSTVYIHENGIRYQSPLRSDSRIDILFSNIKNLVFQSCKGELIVIIHIHLKNPILMGKKKIQDVQFYREASDMSIDETGTGRRSQFRRYGDEDELEQEQEERRKRAALDKEFKYFADAIAEASNGLLTVESTFRDLGFQGVPNRSAVFCMPTTDCLVQLVEPPFLVINLEEVEICILERVQFGLKNFDMVFVYKDFNKPVTHINTVPIESLDFLKQWLTDMDIPYTVSSINLNWSTIMKSLQEDPHQFFLDGGWSFLAMGSDDEASDESEEEVSEYEASEDDPSDESAFSDEDAYSEGEAEFSDDGSEEASGEDESEDGEDWDELEKKAAKADRVATFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4S8</accession>
    <name>A0A8J2T4S8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">Alpha-1,3/1,6-mannosyltransferase ALG2</fullName>
            <ecNumber evidence="5 16">2.4.1.132</ecNumber>
            <ecNumber evidence="4 16">2.4.1.257</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">BN860_17106g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 16">Mannosylates Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 16">
            <text>a beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;3)-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29515"/>
            <dbReference type="Rhea" id="RHEA-COMP:19511"/>
            <dbReference type="Rhea" id="RHEA-COMP:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58472"/>
            <dbReference type="ChEBI" id="CHEBI:132510"/>
            <dbReference type="EC" id="2.4.1.132"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14 16">
            <dbReference type="Rhea" id="RHEA:29516"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>an alpha-D-Man-(1-&gt;3)-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29519"/>
            <dbReference type="Rhea" id="RHEA-COMP:19513"/>
            <dbReference type="Rhea" id="RHEA-COMP:19515"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:132510"/>
            <dbReference type="ChEBI" id="CHEBI:132511"/>
            <dbReference type="EC" id="2.4.1.257"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15 16">
            <dbReference type="Rhea" id="RHEA:29520"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the glycosyltransferase group 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.132" evidence="5 16"/>
    <dbReference type="EC" id="2.4.1.257" evidence="4 16"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4S8"/>
    <dbReference type="OrthoDB" id="448893at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004378">
        <property type="term" value="F:GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102704">
        <property type="term" value="F:GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03805">
        <property type="entry name" value="GT4_ALG2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000222">
        <property type="entry name" value="Alpha-1,3-mannosyltransferase ALG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027054">
        <property type="entry name" value="ALG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45918">
        <property type="entry name" value="ALPHA-1,3/1,6-MANNOSYLTRANSFERASE ALG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45918:SF1">
        <property type="entry name" value="ALPHA-1,3_1,6-MANNOSYLTRANSFERASE ALG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 16" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="507"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="18">
        <location>
            <begin position="57"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="17">
        <location>
            <begin position="253"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CDF87904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="538" mass="61641" checksum="1461D232F4534D5B" modified="2022-05-25" version="1">MFLRGPIVFSAAIRKKYTSIARVTNKVKREPIVQSFAGIMSETKKLSIAFVHPDLGIGGAERLVVDAAVGLQEQGHDVIIYTSHCDKSHCFEEVKSGLLKVEVCGDELPTSFLNKFYIVFANLRQLYLVSGLYFGGKANKHDLYIIDQLSTCMPLLHMLSSAKLMFYCHFPDQLLAQRTSLIKKLYRIPFDIFEQFTMSAADCVVVNSYFTKTIYRRTFKFLNDDPDVIYPCVDLSFQPIDNIDQVFLSGLLRPNERYYLSINRYEKKKNILLALKAFALSSESREDNTKLIICGGYDERVSENVTYLQELQKVADDLKLSYTTIFYPEFRKTKDLRNFTALDSKVIFLTSISSSLKELLLSKMELLLYTPSFEHFGIVPLEAMKHGKPVLAVNTGGPLETVEPLVSGANENEATGWLEKADPKNWAEAIEEFKCISKKGQVNFEKNGLRRVEKYYSRTAMTCSFERNIEKIIYADKQRYTWELLPAGVIFILLQLGFQYFLDGQIWPYLLMIAVTGLYFRSILVLFWIIVTIARFSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4V1</accession>
    <name>A0A8J2T4V1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_06238g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_06238g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4V1"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060963">
        <property type="term" value="P:positive regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.20">
        <property type="entry name" value="Centromere DNA-binding protein complex CBF3 subunit, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052146">
        <property type="entry name" value="HOT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038279">
        <property type="entry name" value="Ndc10_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022210">
        <property type="entry name" value="TF_GCR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784:SF1">
        <property type="entry name" value="GLYCOLYTIC GENES TRANSCRIPTIONAL ACTIVATOR GCR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784">
        <property type="entry name" value="PROTEIN MSN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12550">
        <property type="entry name" value="GCR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription activator GCR1-like" evidence="2">
        <location>
            <begin position="594"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="505"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="531"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="742" mass="84120" checksum="2211918CAE0C5666" modified="2022-05-25" version="1">MNRVPAFFNETRDSSQPLHLPFTSHANELHDVLHAQDREARISSTNISSNFYLITQYILQAYFKVQYGELQGLKLVDLVVDATYGESLTLRKLNENTSVKSYEYFNTVGRQEDITRCPIFALATYFVIRWSHPNPPITLDNFDRVRLLDASAISMDRGFQELAGAGVTDQQGKVTRAESFEPPRELANVIFPWLPSLRQDMELVDRTNYKLHSFLELFEFMARAIVQDLKYLQLHPALLPNIVSFVAKFIPELFHSEKFLSMRTLGSSTGAVGGSQINGASDRHSEQYLELSRRLTTENVRLGQQITQLKTDLDNVRYMCDQILRLQRQVLRQSDSNASTSGIAGGGPSLLSNLVQSAEESTRKRKLPLPTTQVTSPFPLSPGGPIPRAESPHTKRLRSEERPTPSQTALDMLLSRTANSPRFPTLQANPSRGTPRYSSPTTFAIGGSPGAVLPPVPLQPRTTQPEAHSQPRRLEANMSAPNTPADNEAAHEDVLPSAPQPIAQERERPHSVPSPQQSPLPPPPSPPPPLQQSAVASQPAQQLQKEEQQQQQQQEEEESHKPLSPKSTAPADNATVASNKKNGEKLGPNRHIKYKLSRDNKTIWDLYTEWYIGLKGHASIKNLIENYGLRRWKVSDDSHFFPTRRIIMDYIEMECDRGIKLGRFTNPDQRREDIRKIIVGDLEKFRINNGLTLNSLSMYFKNLTKENKEICIFQNFRNWSVRAMTEEEKNKYCKRQHMKENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4X2</accession>
    <name>A0A8J2T4X2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">BN860_18536g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_18536g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4X2"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015793">
        <property type="term" value="P:glycerol transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000061">
        <property type="entry name" value="MFS sugar transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF55">
        <property type="entry name" value="SUGAR TRANSPORTER STL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="326"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="419"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="30"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="541"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="551"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="581"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF87965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="598" mass="66456" checksum="2C569331E80F35BE" modified="2022-05-25" version="1">MGRKVQGYMDRLFSRTSHAGLEGAKLRYTAAAVAVIGFALFGYDQGLMSGLITGTKFNEEFPATKGDDRRATVIQGAVTACYELGCFVGSLFVMFYGDGIGRTPLIIFGACITIIGTVISTCPFHESWGLGQFVVGRVVTGVGTGFNTSTIPVWQSEMTKPNIRGAMINLDGSVIALGTMIAYWLDFGFSFINSSVQWRFPVSVQIIFALVLLFGIIRMPESPRWLISKGKNEDARYVLAKLNGVQEDDDEIYAEMTALIEAVNRTSNQQSQMKSLFSNGKSQNLSRALIASSTQFFQQFTGCNAAIYYSTVLFQKSLKLGHVRSMILGGAFASVYALSTLPSFYLVEKVGRRKMFFFGALGQAISFTITFACLCRPTKQNSKGAAVGLYLFIVCFGLAILELPWIYPPEIASMRVRAATNALSTCTNWITNFAVVMFTPVFIQTSQWGCYLFFALMNFIYLPVIFFFYPETAGRSLEEIDIIFAKAHVDGTQPWRVAHTLPKLTMQEVEDYSNSLGLHEDDAEKEEYDEKHQGANNDALFHMETSSTASNKEDAPVEHREVQESAKDTTNSMPRNLGQHDEDEDEDEDDDEEEEQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4Y5</accession>
    <name>A0A8J2T4Y5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutathione transferase</fullName>
            <ecNumber evidence="2">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_19262g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="2"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4Y5"/>
    <dbReference type="OrthoDB" id="2098326at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03189">
        <property type="entry name" value="GST_C_GTT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03046">
        <property type="entry name" value="GST_N_GTT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000156">
        <property type="entry name" value="Glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF9">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="6">
        <location>
            <begin position="43"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="136"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="274" mass="31207" checksum="4457C263DF5BCCEC" modified="2022-05-25" version="1">MKQGFSIAACRAASFITGTASGIAPTEYLRQSDVKHHNFPMSLPIIRVHFLNESRAFRVLWLLDQLKIDYEIIPYKRNDEFRAPEDLKKIHPLGRSPLLEVEDRQTGKKKILAESGYIFQYVLQHFDKSGSLNNQDPDKAEEIQYYLHYVEGSLQPPLMIEFVLSMVKKAPIPFPFSYFAGKVSDKISEKYSKGEVKNQFDFIEGEISKNEGYLVGGQLSAADILMTFPLDIAFTRKFADPKKYPSIQKWLTTMKALDSYASSKKKAEAVGAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4Z4</accession>
    <name>A0A8J2T4Z4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="14">BN860_00782g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_00782g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1933107at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20710">
        <property type="entry name" value="NOT1_connector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.840">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1 TTP binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007196">
        <property type="entry name" value="CCR4-Not_Not1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055454">
        <property type="entry name" value="CNOT1-like_NOT1_connector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032191">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024557">
        <property type="entry name" value="CNOT1_dom_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032194">
        <property type="entry name" value="CNOT1_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032195">
        <property type="entry name" value="CNOT1_HEAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032193">
        <property type="entry name" value="CNOT1_TTP_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038535">
        <property type="entry name" value="CNOT1_TTP_bind_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040398">
        <property type="entry name" value="Not1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162:SF8">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25097">
        <property type="entry name" value="ARM_Cnot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16415">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16418">
        <property type="entry name" value="CNOT1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16419">
        <property type="entry name" value="CNOT1_HEAT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16417">
        <property type="entry name" value="CNOT1_TTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12842">
        <property type="entry name" value="DUF3819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04054">
        <property type="entry name" value="Not1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 HEAT repeat 1" evidence="12">
        <location>
            <begin position="160"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 HEAT repeat" evidence="11">
        <location>
            <begin position="398"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 TTP binding" evidence="10">
        <location>
            <begin position="584"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 CAF1-binding" evidence="9">
        <location>
            <begin position="773"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1" evidence="8">
        <location>
            <begin position="1075"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1-like NOT1 connector" evidence="13">
        <location>
            <begin position="1366"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-Not complex component Not1 C-terminal" evidence="7">
        <location>
            <begin position="1685"/>
            <end position="2051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1294"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1294"/>
            <end position="1311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF88034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2071" mass="235563" checksum="CF7132004850DEDE" modified="2022-05-25" version="1">MQSSAFANSNEKQEKESLQIISSQISLLITSLTADNYITVEKEIDYLVERSASSVYLKYWRRLLVLCSHDIRSTHKLLPDQNLLHRLLLHLIEDLESKVPSVWQLLYREAFQNVEFLSESGLETSDIEQLLKHYLHGEGKDRTQELEALLSIKMNNSRYLQNFITDYSVETLQNDLTDLLFSLGGETRNDMIALMMSEIISPGSQKIQRDASNSWFTPQSIPEATRVGHEIAKVFKNFTKENVNWNRIFNLMSTKYFLGTPIKPTTASLSSFLACLESGPLIDQFFNCDWDSSFKLQLACLLHHWPLQQMCFDLLKADGIKKVTDMVPNSKQSLLYLMSVASLDLELFLLRDELVNHPMLPYYQECFFEDFNLVPEYLAFALVADIKRFTLLIENGNMIDEILVTLLVQVFEKSPMVMGVLIKSIPDNEKIVDAGKIIVAKENLPIANFVKILADEGKLDYVIARIPFNEVFRILPSARKVGWTGFEDFVKSNLNTNTAPVILDCLDAQTKMTDSNTTFRSSKVFDLAALSFLVNILIDFPLKKFDRDRFEAVQFSLIIAFPRLINFGYGHDQAILADGGLVPFTTEVEKEMQNYLQKMYSGELAIKDIIDVLRKLRDSNNARDQDVFACMSHAVISESSFFRDYPLDALATTSVLFGSMILFQLLRGFVLDVAFRIILGFAKEGPESKMFKFAIQAIYAFKIRLADFPQYCKDLLEHVPRLQSQPQVYQAVIDATVQAGRRASSFVDEVSPTAETLPLKFFNVEEPESQAQQETPPKEIMEKVLFVVNNITMDNFDSKITDLKPVLIPNYFAWFSNYLVNQRAKTEPNYHKLYSRILSSIGSELLHEYMISVTFKQLFVILSTKEAQLIDKNHLKNLSAWLGNITLAVDRPIKHNNIAFREMLLEAYHAKRLDIVVPFVTKVLQSAAESKIFRPPNPWTLGILKVLVELNQKADWKLSLTFEVEVLLKAFGLNPEGVTPSEFLSVSDITDELSGSLNKMTIDQQQSEQQKQMALMQQYQQQMLLFHQRQQRLVNTGMAPPIDQIPMASATADNAALNENPFSNLYGSTIFVTHPDLKRVLQMAIAKSVRENLLPTVEMSSSIAVVTTLKIVLKDFATEVDEVKLKTAALTMVKHLAQSLARATSVEILREGIRSTTQSLAPNLMNLPTSPLEELDTAINDNIGLAIGLVEKAAMDKAVQDIGEQLLQPIAIRRYHKERRADQPFMSQNTNPYSLALPEPLGLKSTGVTSQQFRIYENAGNFAPGVDATNMPAASASNVTPLQQSTNQQILLQAQQPRQPQQAQMGQGNQQVLSHAQSQLPPPTQPVNMSNELEQNHRVLVHLMDGLVVQIKENAEKRSLEGLGNQNPIKAIIFQILTFIARSSQKDQLSLKVSQAVVNSLFATSESSLCREVLSLLLEKLCSLSIVARKDVVWWLVYALDSRKFNVSVIRSLLEVKLIDATELDKVLVSAMKNGMENAVEFSMKLIQDTALSNSPILMRMDFICTLEYLGSLDDTQVKEFFKDYEGRKILPVKKGTLITDSERYYLVFTEWVKLLQRVACDDVVTNVFIQQLMDKGVISSTDRLIEFMRAALELSVFSFKESDPTGEVFTAIDALSKLVIKLMLVQDLSSMRRAEYLDIILSVVALVFSKDHEEQDTTFNERPYFRLLSNLLFEWETIRGHNFVKIKDQKARTDFISFDAEFYKIFSNYLHSLQPIAFPGFSFAWVTLISHRMFLPAILRLPNESGWRNLTLLLIDLLNFLNQYTHKNEIPNAVSVVYKGALRVLLGISNDVPEYLIENHHELINNLPSTYFQLKNVILSAIPKKMILPNPYDPNLSMANIDLCQDPPKVYYDPVNDLKFLKKPVDNYLRIPSNSLLRTIVSGLYKTEYEMRNGVGYDYSTTDVKLVRAIVLHVGLEAGLENQRMSSSAVFNTKSSYYMLLFQLIHEGITELKYQIIQVMIEQLRYPNTHTYWFSYALKNMFSSDDWGEQQTEVQELILRNLLERIIVNRPHAWGVSVLFSQLLRGKDVQLLELPFVKKFPEINVILSQLHKHLSMETVGSQENGATELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T533</accession>
    <name>A0A8J2T533_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ZYBA0S03-10572g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_10572g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T533"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="328"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="462"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="499"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="532"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="558"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="33"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="52"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF89160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="603" mass="66689" checksum="76CC66CE2B399AF6" modified="2022-05-25" version="1">MHRQFQHTTHDRRSSIVYSSSVGKAGLFSPTDYISWENTPDGHENENDATGDAQSDEGSFQTPRQSLLDANNSRTLQRESQNWRGNRRSLPESLIEEERELLADNKMFRNPSDHQNNYGSTDCPTEVMQVWENAVKSGKPIKTTIRRESKVIGMSALPLVVTFLLQDSLTLASVFSASHLGTEELGGVTLGSMTANITGLAAIQGLCTCLDTLCAQAYGAKNYYMVGVYVQRCAVITILAFLPVMLIWCLWAEPILAFMIPQKELCHLAARYLSVLSIGVPGFVLFECGKRFLQCQGIFHASTIVLFVCAPLNALMNYVLVWDERIGIGYLGAPLAVSINCWLMTLGLFLYTIFTKEETRPLKCWNGLIRPDQVFKNWRKMFSLAIPGILMVETEFLGFEILTIFSSYLGPAALGAQSIVSTIASLSYQVPFAISVSTSTRVANFIGASLWKNCIVTCKTSLLLSFVCSSLNMLLTFGFRKQLAAMFSKEEDVTDLVVATLPILAFMQLFDAFNASTAGCLRGQGRQKIGGYINMVAFYAIGIPMAYLLAFPRKMGVGGLWVGITMALITMSVCQGVAVFRCNWREVIDAAKSRNEEVQESAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T555</accession>
    <name>A0A8J2T555_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="10">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_08108g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="10"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19672">
        <property type="entry name" value="UBR-box_UBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.30:FF:000002">
        <property type="entry name" value="Putative e3 ubiquitin-protein ligase ubr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044046">
        <property type="entry name" value="E3_ligase_UBR-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055194">
        <property type="entry name" value="UBR1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF26">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18995">
        <property type="entry name" value="PRT6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22960">
        <property type="entry name" value="WHD_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="12">
        <location>
            <begin position="113"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="9">
        <location>
            <begin position="113"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1811"/>
            <end position="1848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1910"/>
            <end position="1938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1822"/>
            <end position="1834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1918"/>
            <end position="1938"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF88348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1938" mass="222569" checksum="C846E4606B3D0556" modified="2022-05-25" version="1">MSVVPEVQDHLRRTLSSIHDVPYFKDVRGPTDRADMDRVLREMIFRYLHFMISNKGEYLGIMFPGSQKEGQGDKFPHSFDEVLNLHNASVFRDDPFYKFDESKVYHVYKHSGRNCGRKFKVGEPIYRCHECGYDETCVLCIHCFNPKDHDSHHVYIDICSEFSTGICDCGDTEAFVNPLHCKAEEDDSSESSREDDIFLSEEMGSLFQAVLTEVFDHFIDVFNQNIEPLPTLQKEITLKLREMTQQGRLSERAQLLSDLAYENEYLKPGMPAGDESIQELGYSEDLKNYTVIVYNDEYHNYSQATTALRQGVPDNKHTDLLASRIDSEGRATLKCSTELSAVMSGFFSVQTNGLSATLTSWSEYIHQEVCKYLILWLNHCLNIPNLKFQNGFRRAMGEVLCAPYDRASQAVNKTPVVRKYFPNKFNEADPYRYGDLSILAEGNKIPLGHHKELPTSSTHYISATLNEVVSPTHRNYTNSRLQHILYFDNRYWKRLRKDVQNAIIPTLASNATYKPLFCRQVVEIFNHINRSVAFMDREPQLTALRECIVQLFTCPTNAMMIFKNGSFKDVMWSVIDIFVEFSKVEGGSLIWQRVQKSNPTKSYGVVFRQGLYAVETLLSKVTDCNIILKPAEFISVVTLCKLFNGAWKIKRKEGEHVLHEDQHFIPYLEYTTSIYSIIQTIEKVLEHSRDGIEEPLLLNAIRLLTTFLGNKTLMYKLIYDSHEIIKMEVSKQRVAFMNPVHTLYSFLIEKISLRKALEATQECTDFLKVSDFSLRSAVLCSQIDVGFWVRNGMSVLHQSSYYKNNPELSSYSRDIHLNQLAFLREDDDLPRVIYNMLDRWELLDWFSGVVDFDHTIYDDKIAAITQQFIAFVYQVLTERQFFKKFSSTKEKRMYYIKNALIYNLCIKPLSYSRLLRSVPDYLTEDAAELELALEEISDFVEPKGLADSGVFKLKESLFSKVDPLKLLNMENEFETTAKIIKAHLAKDKVTAARVVLQPQIMSPEDIDERAAELGKFTRNDVFAKLIYKLLQVCIDKQEGTFLYELLHLVHGIFKDEELYRGKESIPQAYLSKPICNLLLSIASSKADVFSEYVTRKADFLLESMILKRPAEVFDSLITSFGRQYVDDYKTRKMNHGVNLEEDEKARKKRLIKKRQEKLMAKFNIQQSKFMKENDFQLGGKDSGELDVDMDNKKVTEGEEFTCSLCQDNTSTDLFVIPAYHDQTPIFRSGNILNVKEFATPWHGFYNDDQKLIYDDDKVLENLEVNGVLGSRKVIVSCNHSIHHSCFKRYVQKKRFSTNAFICPLCQTFSNCVLPIRHVSKINTGLSIERLVNEEISVDVLARLLETFSAADFKNVYSTFNLVALHSHSYDKNARSLPGFQMADTAYILSVHWANTISMLEISARLDEPSRGSFLKNREQKYKTLKNTLICIILMCYSVGKPNPEFRPYENKDGIVWNQNQLFQYIVNKSLFSSEPFRETITHALTNYCKQLITEFIKGINPNDIEGMYNKATEFGELHEVQDIELLDTLKEVCVLGLSDPALSAKAYNLAYTSLLKNVLPTLRRCLVMLKCFHELVRGSEEDPFCVNGINFEEDQHLQPLPDYVNIVVSALTEFGSLQLLLAQGIPNMRPVEDTYLVDIPYEFCGIIKLIDLAKYLNTYVTNSKQIKLREEHWVRMKNVKNRLDYKICLTCGVKVHFRTDRQEMSKHLLKYCFKSFGAFLVPNSSEVCLFLSQPTSTVFISAPYLNSHGEAGKNAMRRGDLTTLNLKRYEYLNSLWINNEIPGYISRVMGDEFRVNILSNGFFFAFNRDPPPRRVPTTGNGSDEEDEDEADEAEFYNNRSSDEDERDVDMGENPLGGLFGGPEGGNVRGFLQVFENLQNAVGNGINPDDTQFAAPILQFFPPQFGGLNAQRDIGGGLNEDDEVLDEIPDQQDADENTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T577</accession>
    <name>A0A8J2T577_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cysteine protease RIM13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_11430g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family. PalB/RIM13 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T577"/>
    <dbReference type="OrthoDB" id="167576at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051297">
        <property type="entry name" value="PalB/RIM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46143">
        <property type="entry name" value="CALPAIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46143:SF1">
        <property type="entry name" value="CALPAIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="6">
        <location>
            <begin position="69"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF88488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="697" mass="78689" checksum="EE11742EC62C9B06" modified="2022-05-25" version="1">MDDWKLLNEVRRSLYLPKGGRNIKDVLGKLIMVASKSDDIDFVKSVLIVKEHFDSLHPKHKLLWLTSRVHGDLYPPLSVDIAGPLPWNGITQLANDKSQYCFKLPLKCNAEISEISQCRDIENCSFVASLISMKAHGVEGPRTRKVGDSIYHVNLYFNGSGHRLVSVDTSTVPTDEAGHQLSMCSRNLMDKIVEIAYLRVRANSYASRGSNTAVDTFMLTGYAPEVKSTTQPIMMENLIKLLQAGACLLAVGTGSHPSVLPLPLLHNHDYPIIGADLKGQKVILQDPLDPRLRPEVSFKDVVRGYEQLYVNWHTKKLFTYERTFEFFYNLDKCNRFDTAMEKPLFILENKSDSTQSVWLLLESHLIQGKSIAYLQELQTNLMTTLGAPHEGACDIGLQLLKLRLPRRESKTIFCHSSVSNAYTVHLYTNCNDVTMTRSAAEKFALVIECNADNVSDCYIGSPFYYKNPTFELNIDPRGDPRQVTVSLQLISQNPTDMVNLQIYNMDDDSLQRPILMDTHYAQQRHDRPFIPLFSKVSYKLVCSTYSEQPDHSYRLQVFSSGDASLLSRIRIKQIHVEFGGLPFQLERKVKCVEKRCRISFFTATNNTCFIRVVPPTLSRSVQLRCIVLKDDGIPIYGTENFQSPLPAGIILENCHILGPATYVLLVEVNGIKTELESFFVTLAIGSAKKLITDENFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T589</accession>
    <name>A0A8J2T589_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arginine N-methyltransferase 2</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_09120g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">S-adenosyl-L-methionine-dependent protein-arginine N-methyltransferase that methylates the delta-nitrogen atom of arginine residues to form N5-methylarginine (type IV) in target proteins. Monomethylates ribosomal protein L12.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. RMT2 methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T589"/>
    <dbReference type="OrthoDB" id="19014at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019702">
        <property type="term" value="F:protein arginine N5-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000310">
        <property type="entry name" value="Arginine N-methyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017408">
        <property type="entry name" value="Arginine_N-MeTrfase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051038">
        <property type="entry name" value="RMT2/GAMT_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026480">
        <property type="entry name" value="RMT2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379:SF1">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038148">
        <property type="entry name" value="Arginine_N-mtfrase-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51559">
        <property type="entry name" value="SAM_RMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RMT2" evidence="7">
        <location>
            <begin position="176"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR038148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="393" mass="44791" checksum="DADBA52224099D2D" modified="2022-05-25" version="1">MSQLHNHLAFTRRPITAEEYLPPIKKLLDAGVPATYTLEQVAAFEQGREDEDHPDASNTTPLHIMARSLPQDLNGDEEKVVLDIMNLLFEYGAGWNFLDYEQKTVGDLLLERGQKRQGVLYSRLVDAGVSAELLLRKVNEGEIEFIDPEELPESLQEASLNAAQDAAAEMKQEADTAIDQKAYLDSKLEYTKDSLVTNENRDGVMMDWESSIMYKAADSICPVPDATVLNIGFGMGIIDQAIQSHSPQKHYICEAHPDVLQKMKEDGWYERKGVIVLEGRWQDCLNDLLDRGDVFFDGIYYDTFSEHYDDMLELYDIVVGLLKPGGVFSFFNGLGADRSLCYDVYKNIVELDVSNYGLRCEYDTLSVGERNWEEVKRSYFNCDYYYHPRISFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5C8</accession>
    <name>A0A8J2T5C8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD-specific glutamate dehydrogenase</fullName>
            <ecNumber evidence="4">1.4.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_01684g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">NAD(+)-dependent glutamate dehydrogenase which degrades glutamate to ammonia and alpha-ketoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-glutamate + NAD(+) + H2O = 2-oxoglutarate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15133"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.2" evidence="4"/>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5C8"/>
    <dbReference type="OrthoDB" id="184415at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056365">
        <property type="entry name" value="NAD-GDH_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016210">
        <property type="entry name" value="NAD-GDH_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055480">
        <property type="entry name" value="NAD-GDH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF24">
        <property type="entry name" value="NAD-SPECIFIC GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23147">
        <property type="entry name" value="GDH2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23152">
        <property type="entry name" value="GDH_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000184">
        <property type="entry name" value="GDH_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="5">
        <location>
            <begin position="662"/>
            <end position="933"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF89331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1040" mass="117862" checksum="6ACC986693BBB83F" modified="2022-05-25" version="1">MLPEQTPDIASLSISSISDYHVFDFPGIDLQKEQVIDLLDQQGFIPDDLIEQEVAWFYESLGIDDLFFAKQNADTIASVINSLYASKLDSFAKAKFSGVAGKPQFSIKNKVINKDAALFMESSGSDHSQPYQLDTQIDDLFLDNKDQKSCRLVSFWTPESGLKLTFVYDSVFPEQRQAVTPAKLLQGDIDSISDQTMAQIFSVENKKLYGLLINLVLEREGPVIKTTHSVKDNDEIRLLVAFRRFTTKRYYSALNALFHYYKLKPSKFFLESFNTNDVVIFSIYLNKSRQSEDVLPNTLELSIRQVEREASLLYAIPNNSFQEFYEKREYSPQEAIYAHVAAIFINHFINRLGTDYQALLGQLNIRDTDTTLLEVVSNLKRKLRNETYTQAMIISILHKYHTLVSKLYKNFAEIHYHQDGSKSIEKTLSYQRLSQLEPFKSEEEFDSYLAKLLPNDSPDLLVLKTLNLFNKAILKTNFFITRKVAISFRLDPSLIMPPIEYPETPYGIFFVVGNTFKGFHIRFRDVARGGIRIVCSRTQDVYDMNSKTVVDENYQLASTQQRKNKDIPEGGSKGVILLNPGLIAPEQTFIAFTQYVDSIIDILIKDPLKERYVDLLKHEEILFFGPDEGTAGFVDWATKHARARGCPWWKSFLTGKSPLLGGIPHDEYGMTSLGVRAYVKEIYRALNLEDTVINKFQTGGPDGDLGSNEILLSTKNESYVGILDGSGVLCDPQGLDKVELLRLARERKMVSHFDKNKLGSNGFFVSVDDRDAMLPSGVVVANGTTFRNTFHMEIFKYVDHVDLFVPCGGRPNSINLNNIQSYIDEKTNKCKIPYIVEGANLFIAQPAKVILEAHGCVLIKDASANKGGVTSSSMEVLSSLALNDHDYVNKFIGTDKNKRTELYEAYVAEVQRRIERNAVAEFGQLWQLNQTTHVPISELSNVLSLTINKLNDDLINSTELWLNDVKLRNYLLLNKIIPRMLIDVAGPGEILKNIPESYLKALLSSYLSSSFVYKFGVDVNIGKFLEFIGSLKREEDALKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5D1</accession>
    <name>A0A8J2T5D1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Glutamate decarboxylase</fullName>
            <ecNumber evidence="3 9">4.1.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_13564g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-glutamate + H(+) = 4-aminobutanoate + CO2</text>
            <dbReference type="Rhea" id="RHEA:17785"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="4.1.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.15" evidence="3 9"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5D1"/>
    <dbReference type="OrthoDB" id="5152799at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004351">
        <property type="term" value="F:glutamate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000017">
        <property type="entry name" value="Glutamate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010107">
        <property type="entry name" value="Glutamate_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01788">
        <property type="entry name" value="Glu-decarb-GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321:SF3">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="549"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF88579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="574" mass="65324" checksum="223918FB56E610EB" modified="2022-05-25" version="1">MLHKHTRYQKHPRTLVERHHSEELEDTALLPEQLNKLQVSGAPVEDPSEHAEHLHYNVPQRGVPEQRAYELIHNELTLDGNPHLNLASFVNTSSTELARKLIMENVNKNLADNDEYPQLIELTQRCISMISQMWKCDERDEPIGCATTGSSEAIMLGGLAMKKRWEGRMRAAGKSTARPNIIMSSACQVALEKFARYFEVEAREVPVSSESHHSLDTRNLWDFVDENTIGIFVILGTTYTGHFESIENVSDVLSEIEAQHPDWANKDIPIHVDGASGGFVVPFSFTDEQMAKTNNSRWGFHHPRVVSMNTSGHKFGLTTPGLGWVLWKSEHYLPEELRFKLKYLGGVEETFGLNFSRPGFQVVHQYYNFISLGYQGYSSRFARSLFVARSFSFGLLHSEVVPEGAFEVVSSIHLRISDGRVPQHAHEYWEHPDQYTPGVPLVAFKLSEKFHKQYPEIPQSMISGMLRSRGWIMPNYPLPHSTDGSDKLEVLRVVFRSEMSLDLALMLLQDVEAVLNKAIKSYNTLTHHVQNLHSVDGRTEIYKLLSALSSPDTEDTEPEPEEHKHLKKNYRGTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5E5</accession>
    <name>A0A8J2T5E5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BN860_13916g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_13916g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5E5"/>
    <dbReference type="OrthoDB" id="10253709at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005371">
        <property type="term" value="F:tricarboxylate secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015742">
        <property type="term" value="P:alpha-ketoglutarate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006843">
        <property type="term" value="P:mitochondrial citrate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000053">
        <property type="entry name" value="Mitochondrial DNA replication protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000085">
        <property type="entry name" value="Tricarboxylate carrier, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053017">
        <property type="entry name" value="Mito_Cit/Oxoglu_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46982">
        <property type="entry name" value="CITRATE/OXOGLUTARATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46982:SF1">
        <property type="entry name" value="CITRATE_OXOGLUTARATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="15"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="206"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF88594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="307" mass="33683" checksum="7B0584D4AE2AAD6C" modified="2022-05-25" version="1">MSSQPQELKKKPISFSNILLGAGLNLAEVSTLGQPLEVTKTTMAARRSFSFMQAIRHVWSRGGIFGFYQGLIPWAWIEGSTKGAVLLFVSAESEYRYRRLGLNNFSAGIMGGITGGLAQAYMTMGFCTCMKTVEITRSKATEAGVPSSTWQIFKDIYRKEGIRGINKGVNAVAIRQMTNWGSRFGLARLVEESLREYTGKGKDEKLSAVEKIGASVVGGGLSAWNQPIEVVRVEMQSRAHDPNRPKNLTVSKALKYIYQTNGMKGLYRGVTPRIGLGVWQTVFMVGVGDIAREFVGKMAGEKPVAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5H9</accession>
    <name>A0A8J2T5H9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S04-03642g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_03642g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0507 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5H9"/>
    <dbReference type="OrthoDB" id="7464126at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097422">
        <property type="term" value="C:tubular endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045022">
        <property type="term" value="P:early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051248">
        <property type="entry name" value="UPF0507/Ank_repeat_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24170">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24170:SF1">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G09870)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="2">
        <location>
            <begin position="292"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1100" mass="128334" checksum="8502251F56DE749E" modified="2022-05-25" version="1">MAYNLPVLLNPLVNVVFNCPNPSDSQYKKLFSKLKTEKFILLVPPCDKLLHYSDVVSGTTLIDLCRTYDFVASHVLLLDQDVHSDGSFNVASCQTEFNTLNGRKVVVRSPSTEILTGDGFSLRRRCRIQDVELITNFNDYLRGSENYPLVHVDHPLVGTLIRDVDIPRVSSPDNSNIRENGSKLVRDLTQKSWSSFENILRLHPDWSGILNSYFVRYRNTPLTEGPYEELFHMIIRQVHTKMAADELFQKIPHLYDLIFDYVELNLYDDVWIRIINYYKNEEIDTEPLSILSVNEMETELYQRNYGDFRLQDVTIMEKNLDLAANSFARLPLTHSHAEKANCLIDTLQSLSKERNLHFNADSLPLTMDADTLISFFVLLVCRTQVRNLKSHLFYLRKFSKDENNIKFGILGYAISTFEAVVCYFDDLKGTEKLQRLENACNEAKKLKSLLSDKTVREISDLSQYQDHLKFRTAQGESVLSQCVINGKNEILCELLKNFECIFPLEDILEDETVDGSTLLIQALKYDNSEAARVIVEILEYSCTEEELRIYINKADKHKRTAAHYLTHEVDILQRIGKYIDWKFRDSGGHTPLFTIFRSYDQQNYEEMMTASFRSAEKWYKSNNEQFEFSDHEDEKGNTLLHIIKNNVSILLEYQNIDINCTNKKGMTPLMLYARYNRLDNVRTIVRDPRIILNNFQHPSFLSTFDYARNPLILRELVCQATQFSSSGLAFVHNLKYEAPSWFFHITLKLISKNEYKTVKLHIKTLQGLFHLLLRLYPATFLPLEKALEDLSNLYKSRVSSIAKLETYHFFSMLTDCFNVILREDDSMRRRVLKESRLLSWMNSQNKKKKNSKALEVLKKKIEPEEVSIITSFLRFNHNELSAVKLKLTIMKKLLTFLKLKSNDLTEAYRFLSLFGTEYASKENTRLFEDIEMSSWAFGERATMTCVREVSFLDKCTARLLNQVEQLLHVEIPEWWRCYGEVLEIHKTYKQKFPNMNRSHYDTGIIASFFEGKREKMESRLSSDLAECKQKMKHVGDLISSTHEILAEELNKYMVFKGHFFINGVLRNWVKENIIILKGRLADIHKRAHLRRMDGQTPKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5N6</accession>
    <name>A0A8J2T5N6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_11474g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_11474g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5N6"/>
    <dbReference type="OrthoDB" id="1026733at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF103">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="UBX" evidence="3">
        <location>
            <begin position="337"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="9"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF88490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="433" mass="49628" checksum="52608E0ED5B520CA" modified="2022-05-25" version="1">MDFVRRIIHGGDAPFTPIPGGFPGSDEQTNEPEVEEPPAEETLRHQWSKKTVLGALLRIPFVLLYYIMMWIIVLLSLLRPVCNLSGFYKRKNSRLSDPKTQLNNLLESLENESKRTLNPSMHDVDTPTYTFEALYNLENGSLSSDIVQGGYTDLLNACSEQCKFAIIYLHDSLLDNSMQYVNGLLCSEEFATLIKKYQVLLWFSEVTVSEGLQVANALKIRQFPFLGVLCLKAEKKIEVIGRLEGDIDKYNHNALENILAKGHSKLVQIRQQRQNVALQRLIREQQDSRYNESLRRDQEAARQRDSEREMQSQREQQQQNRKQWLLWRKSQLHPEPSDGSACRIAIRMDGNSRITRKFDSNLRIEEIYAYAELSLEGLLNSSESCSSPPQGYHHHYGFVLISPVPRKQLDPTTLIGDEPAIYPSGNIIMETLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5Q9</accession>
    <name>A0A8J2T5Q9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="15">ZYBA0S04-08196g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_08196g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5Q9"/>
    <dbReference type="OrthoDB" id="1029639at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04044">
        <property type="entry name" value="C2A_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04052">
        <property type="entry name" value="C2B_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04045">
        <property type="entry name" value="C2C_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04040">
        <property type="entry name" value="C2D_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21678">
        <property type="entry name" value="SMP_TCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000226">
        <property type="entry name" value="Tcb1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037761">
        <property type="entry name" value="C2A_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037765">
        <property type="entry name" value="C2B_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037762">
        <property type="entry name" value="C2C_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037756">
        <property type="entry name" value="C2D_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056910">
        <property type="entry name" value="TCB1-3_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017147">
        <property type="entry name" value="Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052455">
        <property type="entry name" value="Tricalbin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980:SF2">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24920">
        <property type="entry name" value="C2_TCB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25669">
        <property type="entry name" value="SMP_MUG190-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037232">
        <property type="entry name" value="Tricalbin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="113"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="14">
        <location>
            <begin position="173"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="367"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="641"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="973"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="808"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="19"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF89602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1179" mass="132495" checksum="C5266E3F76B4EC43" modified="2022-05-25" version="1">MVATESKGSQGAQYSNAGSDEKKNNDTPEVPKKNTEEKPSKTIDEKRAEENISNDEVVREPKEASYVGWRQIGGWQESDTLTAEDDLIDLSKETFLDNVLPDSAYGDWYHSLGIIFASGFLSFLIGYFKFSMASVFFVAVAASLYYRTSAKKYRSAIRDQVQRELTVQKIENDYESMEWTNNFLDKYWPLLEPGVSQMVVQNINPLLAATPYIPAFVKSIWIDQFTLGVKPPRIEHVKTFQNTKSDIVVMDWEVSLTPHDISDMNAKQMRNYVNQRLVIKAVIFGVRIPIYLSDFAMRAKTRIRFKLMTPYPHIDILNIQLLEIPEIDFAARPFGDYIFNGEVMDIPMFWPAIKQLIKVYVGPMLLPPFSMQLNIPQLLSGVYGAIGVLKITIKNAEDIKRSDGFITKTFNPYVSFGLSGEIVARTKAAKDTLNPVWNETKYILLASFTEPLTITAVNERESLKDKAVGRVEYNLSSLHDHHNQKNLSCNFLRNFKPAGTFNFDLHFFPTVSPKRMPDGTVEEPPDFNTGIARITIEEAKGLGKEHENVNAFVELYFNSKLIFTTPSASGDELVQWNQEHENVITDRRKSRYKILVKNSKGNVIGSIVQSLSDLVDRTQTGKKEIALNESNGQIKIGAIWKPVGLDIGTSAIAYTPPIGVLRVFLNKAEDLKNLEKVGKIDPYAKVLLNDNMKERTEAKSSTLNPIWNHAIYVAVTSPNQKLTIEVMDVETVGADRSVGKCDIKVDDMFHKGEDDRYTEFVDDKPRTARLVTRKGMKGAVTYYLAFYPCLPILTLDEMQEIDEARKKGEELQRLQEEAKNKPMSDEEKKTLDEELTRIADVKSVYTNKMKLDLDEILQYNAGVFSVTVLDGEMSQIGVYVQAFFDSGGQPRFVSPKIPTRTVQTGWNGDVMIKELEYSVTTFRVTKNRDSNKAEPCICEVNIPTIELVKNCYYKPSILTLSGEGSAKIMLQVSWFPVAVTKLPQADLISNSGDLKITAKSAENLISADRNGLSDPFLKFYINDDKTPVYKTKHVNKTLNPTWNESGTIEIHNRVNDYLRIKVMDWDAANSDDLIGRVVVPLSKVDPENPVDMDLPIVAEDGGDGGMLHLSFSFAPRYTNNAYKPEKKVGDIASKGLTSGFKAGTTVVTAGFDTIGKIGKTMFGGKKNHYVAQGIYNSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5W4</accession>
    <name>A0A8J2T5W4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_14268g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_14268g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5W4"/>
    <dbReference type="OrthoDB" id="4047468at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018837">
        <property type="entry name" value="TF_CRF1/IFH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28057">
        <property type="entry name" value="PROTEIN IFH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28057:SF1">
        <property type="entry name" value="PROTEIN IFH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10380">
        <property type="entry name" value="CRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="269"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="390"/>
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        </location>
    </feature>
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            <begin position="579"/>
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            <begin position="772"/>
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    </feature>
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        <location>
            <begin position="879"/>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
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        <location>
            <begin position="48"/>
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        </location>
    </feature>
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        <location>
            <begin position="86"/>
            <end position="98"/>
        </location>
    </feature>
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        <location>
            <begin position="100"/>
            <end position="126"/>
        </location>
    </feature>
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        <location>
            <begin position="127"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="177"/>
        </location>
    </feature>
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            <begin position="197"/>
            <end position="219"/>
        </location>
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        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="281"/>
        </location>
    </feature>
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        <location>
            <begin position="297"/>
            <end position="323"/>
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        <location>
            <begin position="390"/>
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            <begin position="421"/>
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            <begin position="599"/>
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        </location>
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        <location>
            <begin position="620"/>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="772"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="885"/>
            <end position="910"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1076" mass="119983" checksum="7AA8058950EEBDDB" modified="2022-05-25" version="1">MAVMKRPLKRPVHNGQVGKKLPGPIARAKGKYPQSNTSGSVTRPRRFSLLYSSDSSMSEGSDVDVKGQHRRRSDRAKSISSNAGGKKSKLIRPFKRNSHSSEQAVSSGSESGIESESQSESENYSGTKESGNATTSNGMNMDEVESEDDDDDDDDDDDDESDSESESDDTSSSEEDIDFVKLSAQRKKRSMKALSALKREGSNFSEDRDPKKLATERRQASKKVLSPVKKPVTTAVATFGKKPGNSPASSDSDEGSVIKEALDFRFRKDDDGIRFSRHGNENSEEDIGEEVQEHNESANNGYNNSNSNNNNNSNSNDTNSIQSPMDDNLNQLHVPHFSDSEDSEYDIDQDAYFNAIDDEDDIADMDAGLAATEDEDVPVLREEENLMSVLQNQDEISFDGSVQESGSDPADMLSASGPDQNNDDGDGSDDDDDEMMTVFDMPFYEDPKFASLYYCEDGSEPRLTLSTSLPRLISDEKMIRMKRREAKKLERRERIKRRKHLKERGSRLKKSKAKNLRPDLSNEEYMFGIFLQSDQDEGGTGEVHQDLRKRLDFGSQLRRLGSAAGSDVSSDDEYDNILLDIANMPSSDDSEDDTLENEEAGREFSETQHEVNTGSASDSLELDLGDSDDEDDGDDDFQDESSVTNVFIDIDDLDPDSFYFHYSDDASTSYSEMLSDDTDAEKGKREEQKEDVLDTVTYANDESTDEDDNLPPPSSRGKAIGSKAKEVVSANVVGLKPPKLGTWETDSKPFTIIDGLSTKSLYPLIQEHQHFLEQQQRAQSQTPEYLSSHEASAGNGDELTLNELLNMSELEDDDATAPSYSQAVSDWYQRPAVPLSAFRNKGVNSHEDDEYILPANSTRRVPIGYIGSERTRRKIDKVKELQRKKTEKKRKLKRRKKLLRMKREKQRIDKKHNMQNPEAAGQEKDEETLQLPDSEFMMSTPLDKPQGHVDALGNQNSRKNSVKSVGLAEIHEILGNDNSDLLENNLSIFDTQADGETAIEVTDAEILASLTAPIELGSLNRRPSWRRRQSMVEAAAENLRFTKNGLFSESALADIEGFINNNGVTSDAFEFSETLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T600</accession>
    <name>A0A8J2T600_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ZYBA0S04-09714g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_09714g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T600"/>
    <dbReference type="OrthoDB" id="5598268at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001002">
        <property type="term" value="F:RNA polymerase III type 1 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001003">
        <property type="term" value="F:RNA polymerase III type 2 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.160:FF:000005">
        <property type="entry name" value="TFC1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.160">
        <property type="entry name" value="TFIIIC, subcomplex tauA, subunit Sfc1, barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019136">
        <property type="entry name" value="TF_IIIC_su-5_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040454">
        <property type="entry name" value="TF_IIIC_Tfc1/Sfc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041499">
        <property type="entry name" value="Tfc1/Sfc1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042536">
        <property type="entry name" value="TFIIIC_tauA_Sfc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13230:SF5">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13230">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIIC, POLYPEPTIDE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09734">
        <property type="entry name" value="Tau95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17682">
        <property type="entry name" value="Tau95_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="Transcription factor IIIC subunit Tfc1/Sfc1 triple barrel" evidence="7">
        <location>
            <begin position="33"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor IIIC subunit 5 HTH" evidence="6">
        <location>
            <begin position="222"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="525"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="567"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="541"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="579"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF89664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="641" mass="72949" checksum="4305D5286DDDA830" modified="2022-05-25" version="1">MVEESSLARLSPTPEYSGALAKEYTLDIPRIPSLELPLRVSSKPQSVRKAVQMCGGLQHVKEALNERRGLEDAQKGLELYLNEGKDAHGSDQFFNEHPIIGKRVPFRDESVILKIQVPQGTMARFGGDVKQALASLNSKEVRVTPVAIVNNTIKFREMSDFQIRLDNVPSAKDFKNSFGSLEWGNFKKYVESVPDNDSRPFENISKMTVNRSEKCPSNDFQLPPPPRFSMVGLPLLYKYTTNPFAKREANGTTEVSSTYIRNYQQFVHDVAEGTSIPVKPHELLQKDFATAQATGVYPGTKKESKFYESLLECVEILKRLFEERSVWVKRHLDGIVPKNIYHTIKVALALVSYRFTTGPWRNTYIRLGVDPRSSPEYAKYQTEYFKIERKMLKSPVASKNIPRPPPPVFESDAPGDIDSRFKFNGRQIPWYFMLQIDLLTDEPNIREVFDKAQFLPQANELTGWFDELDLAKIRRIVKYELGCMVQGNFAFNKYKLKYYKSMLYVKESMITGQKKDTEGDIDMAELNHDKQSISDTATPNDIEEAEEDEDNGVATGEADEVVLAAEEADEDDNIRVDVPEGDDEVTNNHDDDEDAADADDDEFDPKSATFQEIIERISKINSETAQRLARDLNGFIYETKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T620</accession>
    <name>A0A8J2T620_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_15544g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_15544g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T620"/>
    <dbReference type="OrthoDB" id="5419460at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032185">
        <property type="term" value="P:septin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36414">
        <property type="entry name" value="PROTEIN SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36414:SF1">
        <property type="entry name" value="PROTEIN SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="118"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="149"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF88664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="325" mass="35510" checksum="A2DDE83B653F0D5C" modified="2022-05-25" version="1">MARMATSIWSCIVKASTLIFFAGNVLLLLLIIISGGSDNYPMSRLYWVEGDTSGIPHASNTTRWTYWGACSRSNGRTNCGGSLGPAYPISPVDNFGTKTNVPHKFISNRGSFYYLTRLAFSFFWIALSFIAISFLIYVGAWFSSAVSHVTWILTGVGCLFNIVAVIFQTAAAVMARDAFTDDKRHGKLGAPLLGIAWASVALCLMEFTSLVAGHIVWSRPSKDERNHEKAELPSVKITPTSRSSSTRSVHPFRTFFLGQRNARTYDENLSKQRDAETGYTISSGTTEGANGTESREPATHFAETRGSNISSDHSAASVDRDLESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T684</accession>
    <name>A0A8J2T684_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATPase synthesis protein 25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_07250g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial mRNA stabilization factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion inner membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
            <orientation evidence="1 7">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ATP25 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T684"/>
    <dbReference type="OrthoDB" id="107372at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140053">
        <property type="term" value="P:mitochondrial gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048255">
        <property type="term" value="P:mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040152">
        <property type="entry name" value="Atp25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025210">
        <property type="entry name" value="ATP25_mRNA_stabil_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28087">
        <property type="entry name" value="ATPASE SYNTHESIS PROTEIN 25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28087:SF1">
        <property type="entry name" value="ATPASE SYNTHESIS PROTEIN 25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13929">
        <property type="entry name" value="mRNA_stabil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02410">
        <property type="entry name" value="RsfS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="ATP25 mRNA stabilisation" evidence="9">
        <location>
            <begin position="268"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF89016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="582" mass="66497" checksum="C7ABFEC4D6E3FB6C" modified="2022-05-25" version="1">MLKASKGLPRLRLLATMGCKRTVLLQSAAIRCLSLSSIVCQERSSPKPWYLNVGNRERELKQTIFQAQDISLPSNSPASLVKISNFLRDELGLTDIVVFDLREGVFETAAAKMCNFMVLATAGSPKHCQTSFQRLNSLIKREYESFAHVEGQYNAKEQRKRQRRLARRPNLASNSESKVPTESWFMIDTKVDRIFVNILTHKRRQEINLEELYAPEEEKAKYVRSQPEKSATEVNSEDNILAGLRRLVNQRREYSTVAPAALVSEKLLQNLQSQDYPSAFRLINNNKDQSLQLMQTLVEWVSNLPIEEEAAVPWSKWVTAFESCWPLVLPEEKASQYWSLRLKFFKLVNMADMKHYNLSNFFNDYLLAKKATGAGLVPQDLVEFLKLAIVNTRYCDKHRGYWDLVHSNDIVVKALRLFEDVRGADAIVKDSSVVTMLLRTMVSGQEIGERATRLHAVYEVSDYLVDTFGPELTPPTIAGILEVLAAAKDWNKYLQFWEAGIGGLVPGADQRPWAHFIRLVVESGDVEMMRKLLSEGHLLWLDRLQVEIDDELKAQLGLLFAKADPEQLWSQELQDHLLLQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6A8</accession>
    <name>A0A8J2T6A8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">JAB1/MPN/MOV34 metalloenzyme domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_02872g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1931567at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097157">
        <property type="term" value="F:pre-mRNA intronic binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000393">
        <property type="term" value="P:spliceosomal conformational changes to generate catalytic conformation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08056">
        <property type="entry name" value="MPN_PRP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13838">
        <property type="entry name" value="RNase_H_like_Prp8_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.230:FF:000001">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000002">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1570.40:FF:000001">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1570.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.40">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012591">
        <property type="entry name" value="PRO8NT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012592">
        <property type="entry name" value="PROCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012984">
        <property type="entry name" value="PROCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027652">
        <property type="entry name" value="PRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021983">
        <property type="entry name" value="PRP8_domainIV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043173">
        <property type="entry name" value="Prp8_domainIV_fingers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043172">
        <property type="entry name" value="Prp8_domainIV_palm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019581">
        <property type="entry name" value="Prp8_U5-snRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042516">
        <property type="entry name" value="Prp8_U5-snRNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019580">
        <property type="entry name" value="Prp8_U6-snRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019582">
        <property type="entry name" value="RRM_spliceosomal_PrP8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11140">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11140:SF0">
        <property type="entry name" value="PRE-MRNA-PROCESSING-SPLICING FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08082">
        <property type="entry name" value="PRO8NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08083">
        <property type="entry name" value="PROCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08084">
        <property type="entry name" value="PROCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12134">
        <property type="entry name" value="PRP8_domainIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10598">
        <property type="entry name" value="RRM_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10597">
        <property type="entry name" value="U5_2-snRNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10596">
        <property type="entry name" value="U6-snRNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="JAB1/MPN/MOV34 metalloenzyme" evidence="8">
        <location>
            <begin position="2159"/>
            <end position="2291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="64"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2394" mass="276440" checksum="CFEC9F3D5353F76C" modified="2022-05-25" version="1">MSGSGLPPLPPPPPPGFDDDLPLPPPPPPPTDGSENGLPPPPPPPPPGYEDGSLTMELISPPSPKEKEYIHDHPAPESKEPKLEEGHKRKREECKGLKTSKKRLKKLRKDRMGQVQSLKPELPPEHLRKIIESHTDMTSKRFNYDKRAFLGALKYLPHAILKLLENMPQPWEQVKKVKVLYHVTGAITFVNEIPRVIKPVYITQWSTMWLAMRREKRDRTHFKRMRFPPFDDDEPPISYADNILPIEPPDAITLNLDNYEDEPVREWLYDPKPLIEDSTKVNGSSYKKWYLPPLIMANLYRLATPLKSEIYDENHYYLFDKKSFFTAKALNNAIPGGPKFEPLYPREEEEDYNEFNSIDRIIFRVPIRSEYRIAFPHLYNSRPRSVQISWYSKPVCCIIKNDEDPDTPAFSFDPSLNPITKLSREHSQGIEENFGLDPTFGPLLLDEDLELPETKDAIELYNAPHPFNKNSGRMVRAEDVALVKKWYLERSNEDFPLKTRISYQKLLKTNVLNELKQAKPADVRKIKLLKSLRNTKYFQQTTIDWVEAGVQLCRQGHNMLNLLIHRKGLTYLHLDYNFNLKPTKTLTTKERKKSRFGNAFHLVREILKVIKLLVDAHIQYRLGNVDAFQLADGIYYILNHLGQLTGIYRYKYKVMHQIRACKDLKHVVYHRFNKIIGKGPGCGFWQPAWRVWIFFLRGIIPLLERWLGNLLTRQFEGRNNEIVKTTTKQRADAYFDLELRASVMKDILEMIPEGIRQDKAKTILQHLSEAWRCWKANIPWDVPGMPQPVKKIIERYIKAKADGWISGAHSNRERIKRGAHVEKTVVKKNLGRMTRLWIKREQERQQQIEKNGPEISPDEATAIFSSMVDWLEKRDFSPIPFPPLTYKNDTKILVLTLENLKDAYGSKVRLNSAEREELALIEEAYDNPHETLNRIKKYLLTQRVFKPVELSMVDHYQTISPVYSVDPLEKLTDLYLDQYLWYEADERKLFPNWIKPSDSEIPPLLTYKWCQGINNLDGVWDTSKGQSTVMMETTLEEMAEKIDFTLLNRLLRLIVDPNIADYMTAKNNTIINYKDMSHVNKYGLVRGLQFASFIYQYYGLMMDILMLGPERAMDFAGPANDPNDFLQFKHPTVETRQPIRLYARYLNKVYILFHFKENEADELTDDYLSENPDPNFANAEGYNNKKCWPRDARMRLMRQDVNLGRAVFWEIQARIPNSLARINWGSSFVSVYSKDNPNLLFTMCGFEVRILPKQRTDEVISTGEGVWDLIDEKTKQRTAKAYLKVSQEEIEKFDSRIRGILMGSGSTTFSKVAAKWNTALLSLFTYFREAIVATEPLLDILVKSETRIQNRVKLGLNSKMPTRFPPAVFYTPKELGGLGMLSASHILIPASDLTYSKQTDTGITHFRAGMTHDDEKLIPTVFRYITTWENEFLDSQRVWSEYAAKRQEALQENRRLAFEELEGSWDRGIPRISTLFQKDRHTLAYDRGHRIRREFKQFSLERNSPFWWTNAHHDGKLWNLNAYRTDVIQALGGIETILEHTLFKGTGFSSWEGLFWEKAAGFEDSMQLKKLTHAQRTGLSQIPNRRFTLWWSPTINRANVYVGFLVQLDLTGIFLHGKIPTLKISLIQIFRAHLWQKIHESIVFDLCQILDGELDVLQIESVNKEAVHPRKSYKMNSSAADVTINSVYEWKVSNPSLLHAANDDFHAALTDKMWFDVQLRYGDYDSHDISRYVRAKFLDYTTDNTSMYPSPTGVMVGIDLAYNMYDAYGSWFNGLKPLMQNSMKTIMKANPALYVLRERIRKGLQIYQSNVQEPFLNSSNYAELFNNDIKLFVDDTNVYRVTVHKTFEGNVATKAINGCIFTLNPKTGHLFLKIIHTSVWTGQKRLSQLAKWKTAEEISALVRSLPKEEQPKQIIVTRKAMLDPLEVHMLDFPNIAIRPTELRLPFAASMSIEKLSDVVLKATEPQMVLFNIYDDWLERVSSYTAFSRLILLLRGLKTNEEKAKMILLGDPTIPIKSHHLWPSFTDEQWIDVESQMRDLILEEYSKKYDVNISSLTQTEIKDLILGQDVKAPSVKRQKMAELEAAKTRPAQDELDAGACTVMKTKTVNAQGEEIVVVTSANYENQAFSSRSEWRKNAIASSLLYLRLKNIYVASQEFIEEKNVFVLPRNLLKKLVEISDVKIQVAAYIYGSSPTDHPNIKEIKTIALVPQLGNGKAVQLSKIPLQEDTPELKELELLGWIHTQTEDVKFMTAHETATHSHLFSDIKRDAIDLTVCLVPGSVSLAAYTLNDDGYSWGLQNKNVLDVTPEEFDPSFSVHAQLLLSERIMGNCLVPSTDVWNHAFSGATFNSELDYELKVGIPLEFYNDMHRITHFMQFSEIAGDEELEAEQASVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6E9</accession>
    <name>A0A8J2T6E9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Carbon catabolite-derepressing protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">BN860_10132g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6E9"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12122">
        <property type="entry name" value="AMPKA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14079">
        <property type="entry name" value="STKc_AMPK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14334">
        <property type="entry name" value="UBA_SNF1_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000544">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000069">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000236">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000017">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032270">
        <property type="entry name" value="AMPK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013896">
        <property type="entry name" value="SNF1_UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16579">
        <property type="entry name" value="AdenylateSensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08587">
        <property type="entry name" value="UBA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="51"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="406"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="406"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CDF89141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="631" mass="71416" checksum="348B675F0F2CE3C2" modified="2022-05-25" version="1">MNGAAGANSAGVSSSGSAKVQHRHHHRHHHHSHGTSAAQQPLADGSHIGNYQIVKTLGEGSFGKVKLAYHITTGQKVALKIINKKVLAKSDMQGRIEREISYLRLLRHPHIIKLYDVIKSKDDIIMVIEYAGNELFDYIVQRDKMSEDEARRFFQQIISAVEYCHRHKIVHRDLKPENLLLDEHLNVKIADFGLSNIMTDGNFLKTSCGSPNYAAPEVISGKLYAGPEVDVWSCGVILYVMLCRRLPFDDESIPVLFKNISNGVYTLPKFLSEDAANLIKRMLIVNPLNRISILEIMQDDWFKVGLPEYLLPPELKQQQQQQDDGSANHEEDDGGVNEEIDDQLVTALSRTMGYDRDEIYESLQSSNRTPVLNEIREAYMLIKENKSLIKDLKSDKHKSDELDTFLAQSPPTFQQRGSNDVSNSTSTAQQQNHQHHERSSSKKAAAQQKACHVAPFVDQNNVEDSTISILPTSLPQIHRANMLAQGLPAASKISPLVNKKSKTRWHFGIRSRSYPLDVMGEIYVALKNLGAEWAKPSEDDLWTIRVRWKYDVGQNHNERIPDLMKMVIQLFQIETNNYLVDFKFDGWESSYGEVPHNPNLSEDEMSTFSAYPFLHLTTRLIMELAVNSQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6L9</accession>
    <name>A0A8J2T6L9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Autophagy-related protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_00144g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)</text>
            <dbReference type="Rhea" id="RHEA:38571"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:38895"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Preautophagosomal structure membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATG2 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="18982at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061908">
        <property type="term" value="C:phagophore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061723">
        <property type="term" value="P:glycophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026849">
        <property type="entry name" value="ATG2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13190">
        <property type="entry name" value="AUTOPHAGY-RELATED 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13190:SF1">
        <property type="entry name" value="AUTOPHAGY-RELATED 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13329">
        <property type="entry name" value="ATG2_CAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF89263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1557" mass="174167" checksum="A0B10EEAAF72588F" modified="2022-05-25" version="1">MAFWLPQMIQKRLLLYVLQQVSVFSSVDLSNLHVSLGSSSQFSFNDVNLVVDEISIPGLDMQSGTITHLDIRLNVSGGVSVYGKGIELVVRPQCSTQDSDSGNFSLAKSIQDLTNSIMQFKDSNQTGVSNVENELNSEFLTSPATTSSSSSLSSREEIEAAPAVSTLETVKNRILNVALSKLMITFEDVTFKLLFEDYEVIEVKLEKICLKTGENNVRKITVHNLDAIKMKTNVDVPKNLSSTIYYSQSESASMYMSAMESPKNEPVDSNEEQSGSVTDLIRVNSIDISFEGLASIEDLSLRDLMVDVDIIRIFADNWLDVGESVLDFVIAVLSKEPTDKTSQPSGLTGYKRFQKEQDILEGIAFLAIKSNVIQMDLAQNFTLLLRTVNLDSLGNKEYVVTVDSFEFRGDGLSSSHPKSPIIRGIIKPKESRLSILHDVNISLTIPALEELLTFSQRITEFLSSFESKLSKKSLLKKKSSEGMRFLTATRCINITLPLAKYDLILKVENAGSNTSSDIFKTNSLKILRRRLGQIDVLLNISGVCVTLSKSRIQLDAYDENLDDSLLTSKILCTIKDITLEDDFVMLQGLLDEFRHLSLLFSAPRRKKDKERKNSYLKRSVRILHSSNLIYKNTAAASFALMVDSIKLRLKSFLKREFGTLDGKLTDITFATTQEGNFVAFAKHLILNRLFGDNEQAIVSPIKPTINERPIFFFQRKNNGRTRITLCNLSFHYYAKWLDLFSERNSKSKRPTSNEKSNAKKELPSNAFAWELKLIDCSVFLLPYRLNAGLMLVVDRMAMNGKSFFLQSKSLLNSGTLLLVDDVCNIKRQDNKEWSSLTSLYAHQGFSAIGKIDTLSIITCYANSTISLHVKLHNLMLSLCADSAHTLIQLCIDLKLPVTFPDEDKYNYNDAEVADVFNEIDPDFFNSSHIKQEGKINNKDDLIVVEDIFLDGAKPRDVANEINSIGSIDETTIDTSSDFTSRTINFHEGYLDIDRGEEETQVEDHEQDIEFKIEVDVEKVSIKLFDGYDWKYSRNFISLTIDQLDREVKAIEGEHPVDGKVQMSVFDSIYVTADTSDDVDLKKRVSEEIQVEKKSSVSIKKANLHPSRFYKALISLEKIKLSFTGYRYDNPSKFESDGSADTLNKCDLSVQKFEILDNVPTSTWNKFMTLSRHEQWSRDRAMLCAQFSTVRPIDYLMATEMITKLEVAPLRLHVDQDALDFLVKFGEFKDKRFELIDDYPDTLFMQKFTTNSVKLKLDYKPKKVDYAGLRSGHTSELMNFFILEGAKLTLKGVVLYGVNGFPDMASTLKAVWAPDIASKQLPGVLEGFAPVKSIAALGSGVKALVTVPLTEYRQDRRLGKSLKNGCNVFLKTATGDFVKVGVKLASGTQAILENTEGIFGGVGVDGRLPRSEEPVVNMDSLLKEDQLVGGSNPKIRGQRPAALVIDPYKTDGGQPKIVSLYADQPLDLHKGLEEAYLSLEKHMHLVYDVLWKTKGEIEDCQAGTAAAAVTAAKVAPVAIIRPVIGATEAVVRTLQGISNQFDKDQIRGFNDKYKSQTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6N7</accession>
    <name>A0A8J2T6N7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ZYBA0S05-04456g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_04456g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF89905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001262">
        <property type="entry name" value="ABC transporter CDR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005285">
        <property type="entry name" value="Drug-R_PDR/CDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00956">
        <property type="entry name" value="3a01205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="490"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="521"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="575"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="602"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="633"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="739"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1246"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1296"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1325"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1448"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="121"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="839"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="28"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1114"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF89905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1485" mass="168373" checksum="BBA567B0C6C47CD0" modified="2022-05-25" version="1">MEASEYGGFDHNAENEVKKMAKALVKENSNNRPLDSSSISRVTSGINPVGLQETDTGYNAKLDPNSNDFSSEEWTRNLANIVESEPEYYKPHSVGCCWKNLSAAGDSADLTYQTTFGNLPVKIFSLLYRHIRPSKGSDNFQILKPMDGLVDPGELLVVLGRPGSGCTTLLKSISCNTHGFNVGKDSVIKYSGMASKDIRRNLRGEVAYNAESDTHIPNITVYQTLVTVARLKTPRNRIRGIDRESWAKHIAEVTMATYGLLHTRNTKVGNDLIRGVSGGERKRVSIAEATICGAKFQCWDNATRGLDSATALEFVRALRTQAKINNSTACVAIYQCSQHAYDLFDKVCVLYGGYQIYFGPTSNAKKYFESMGYYCPSRQATADFLTSITSPAERIVNREFLERNIVVPQTPDEMYLYWQNSPENHELQREIDSRLTQDYKENLTAIKASHNAAQSKRARKSSPYTVSYAMQVKYLLIRNMWRIINSPGVTLFRVFGNIIMSLVLGSMFYKVQKTTTTNTFYYRGAAMFYSILVNAFSSLIEIFALFEARPVTEKHKTYALYRPSADAFASFLSDIPGKVVSTVAFSIIYYFLVNFRRDAGRFFFYLLINLVTTFTMSHLFRCVGSISKTLTEAMVPASVLLLTLAVYTGFSIPRRSMHEWSKWISYIDPLSYLFESVMTNEFHGRNFPCAAYIPNGPQYQNTTGTERVCSVVGSKAGHDYVLGDDYLKQSYEYEIKHKWRGFGVGMAYVVFFFFVYLLICEYNEAAKQKGDLLVFPRSIVKKMRRQGTLQKSNSEPEDVEKNATIIANVSPDSSLLLDTLESPSKQKSEIPLNQSDTIFHWRNVCYDVHIKKESRRILNNVDGWVKPGTLTALMGASGAGKTTLLDCLAKRVTTGVITGEIFVNGKLRDESFPRSIGYCQQQDLHLKTATVRESLLFSAMLRQPKKVPLSEKKQYVEQIIEVLEMVPYADAIVGIEGEGLNVEQRKRLTIGVELVAKPKLLLFLDEPTSGLDSQTAWSICQLLRKLANRGQAVLCTIHQPSALLIREFDRLLFLQKGGKTVYFGELGDECKTMIDYFERNGAHKCPPDANPAEWMLEVVGAAPGTHASHDYNEVWRNSEEYKEVQQELDRMESELKCVGEEDSSEKHQAFATDIFSQILIVSHRFFQQYWRTPQYLWPKFILTAFDEIFIGFTFFKATRSLQGLQNQMLSTFVFCVVYNALLQQFLPVYVEQRNLYEARERPSRTFSWFSFISSQILVEVPWNIIAGTIAFFVYYYPVGFYANASEANQLHERGALYWLFCTAFFVWIGSMGILANSFLEHAAEAANVALLCFAFSLAFCGVLVPPKLLPGFWIFMHRVSPLTYYIDSALSLGIANVDVKCSEIEYVKFTPPSNRTCGQYMQAYIKSIGTGYLADPSATNECNFCRLSKTNDYLKQISSSYSHRWRNYGIFICFIVFNYVAAVFLYWLARVPKREGRVSKKKQEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6W5</accession>
    <name>A0A8J2T6W5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BN860_08658g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">trans-Golgi network membrane</location>
            <topology evidence="12">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="242910at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13980">
        <property type="entry name" value="STKc_Vps15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000497">
        <property type="entry name" value="Phosphoinositide 3-kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045162">
        <property type="entry name" value="Vps15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583:SF0">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="27"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="15">
        <location>
            <begin position="609"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CDF89621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1413" mass="159203" checksum="B05A99D6FF94550F" modified="2022-05-25" version="1">MGAQLSLSAQTSPSIAIFSYVDVLDEVHYVSQLNSSRFLKTCKGLDPNGEIIIKVFIKPAEDYNLKKVQKAIEEEAMMLAPLPNALNFSKVIESNRAGYLIRQHLKSNLYDRLSSRPHLQNIELKFMTFQLLQALNDIHTLNITHGDIKTENILTTSWDWLILTDFSSAVKPVYLPEDNPGEFSFYFDTSKRRTCYLAPERFDSAQYLKTKGRAGPVTKEMDIFSMGCCIAEMYAEGRPLFNLSQLFKYKNDDYDVSGLLTEEIQSEPLRKLIMDMIQLDPRRRPSSRQLLKKYRGSFFPEYFYTFTYEYLRALSTFGTGTPTSGSIYAHTTIEDQLTVIDKCCEKIYAEFGKICASLGYKLDLNNEGERERDSHDWDLFFSFSVRLSDCGSLKLQDIQTNTAAIQEESILLFISYICHGLRHIASSNTKLRCIEMLTAFSQFLSDENKIDRVIPYLVTCFEDEYSNVQALAVQSLSQVLYTIKRVNHVNENFFLDYLFPRLKRLLQLSKQNTYVRMVLANCLGDLVTVADRFQELAYFSSSTNSQEGIVHGMESLEITSKQTKKLVQQIEDLVVTLLTDTETKVKMSLLANILPLCKFFGREKTNDVVLSHLITYLNDKDSSLRIKLIQSISGVAVLLGPITLEQYILPLLIQTITDPDELVVANVLQSLKDLCKTGLVEKRFFYDICTTISPLLLHPNNWIRQFSLLLIVEISSKLTKAEVYCVLYPIVRPFFEFDVEFTYELMLSSCKSPVSRTVYNLLCSWLLRTSKSLFWQQVPNKHVDSFGNSSITFITKDYSSKNYGFNNVIKASNSTVRSFDKKEIPLTTEDKNWIDKFKAIGLPESDLWKIAILRGYVVRTAKLVARTREASLGNGKNNSKLENQASVTLANVMPRTVFFDVEFVESEDSTSPSVGDDLHLDYSGKGNKALPDVKDFDGSLMFNSKAIPTTTPSLKNIYVQLEPTSHHKEIPNHLLERPKVEPRLVIHNSYEGDAPTIKHFLSRITVHPSMKEYEEFGPVKTIFENTCIVKNINGLLSATLTENEPNAIVAMAAANGEIPYLITGTINGSIKLWDISRTASGEMYSSVHSKDLASTITDIAMLQGYDAFCISTKEGSLSVVRVLLQQRKLTGFQTIRRVTINEISKDEFAIGVKTVLTDDKAYLFALTNTSKLVVYDIRTMDCISTVESPVSHGAACSFVPDEQGDVLIVGTTKGIIEVWDMRFKLLIKCWTFGDHTPITHLEKCNALGKNCIVVAGGSTEAVLSIWNYCKGQCQQALINSDERPSVEHFLPIEKRLDEIVFSKASQKLQSVGSICVNGSRILVAGGNSNDIILIDVKDLASSRVLVAQEGWSSTFVPVQVTAHLTFLLKKNSHYQTPPTFCNDTINCLELVCTRNNTPLVVAADNSGVITYIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T745</accession>
    <name>A0A8J2T745_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S05-05006g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_05006g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF89930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T745"/>
    <dbReference type="OrthoDB" id="5572108at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10206">
        <property type="entry name" value="ASKHA_NBD_Arp8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="884" mass="99250" checksum="47F285CA8678953F" modified="2022-05-25" version="1">MSQEADSGTVYEEPIEIPLEEEDDDDEEDDVGETAGDSRADTQDEAHPEDDEEADEGDGDPEGEGEGDADGEGDGDPEGEEDMHGTASRSGTATPLGHGNNGEGDEDDMEDEEVDEDDGDENGKRRTSRGKFKKYPKLDPAKAPPGKKVPLHLLEKRRLGRIKAAEEFAKKLKKIGIEKVESNTLPSTGLFQPMLLINQKNYSSDYLKKDEQVFALRERKVLRNNTQVSSMANTPDVADLKKENGQAELNETNGDEDIDLSDANTTLVIHPGSDSIKIGFAKDETPMVIPACVAVPKSDLLGEVSNQESKRNPYGRQQPADFEELKSDIQQSFKERMRFYKRKVQPNAHEQVVTFNKQSKPEYMEEKNDFNRINWITTPDRRYYGKDALRCSKDHFIVRRPFVKGGSFNIESSDYRSLQDLLGDVTGLLEYTLGSQHFELKPSQFSQYKLVLVIPDLFEKSHLETFIGCLITELQFQAVAIIQESLATCYGAGISTSTCVVNIGATQTRIACVDEGTVLENSAITLDYGSDDITRLFAIFLLQSDFPYQDWDIESAHGWLLAQELKKNYITFQDANVTVQLYNFIKRVPGKKAEKYDFKVFDEVMLAPLSLFYPEIVEQLQSESSPKVSNTKLQDQLPVSRDIFTDNLNDWRSMSQAECLNGELYCGVQDDFQTLCKVLDSQSKVEESQTENITVADTRKNYAPLEKAVVQSITNASLFLDIAKMAPFYSNILVCGGGSKIPALDFILTDRINIWRPRLLSQNSFPTFYKKLAKQIKDLKAAGKNSEGKTSALPEEDQSQEKVNDIIQKEFQKYMETIEAQGNGDHTLPVSVLPPPRDMDPAMLVWKGASVLAQIKLVEELYLTGTDWDIHGSRILQYKCIFTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7A1</accession>
    <name>A0A8J2T7A1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 20">DNA topoisomerase 2</fullName>
            <ecNumber evidence="7 20">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">BN860_08218g</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18 20">Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6 20">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 20">Belongs to the type II topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="7 20"/>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="276498at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000819">
        <property type="term" value="P:sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16930">
        <property type="entry name" value="HATPase_TopII-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03481">
        <property type="entry name" value="TopoIIA_Trans_ScTopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03365">
        <property type="entry name" value="TOPRIM_TopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.40:FF:000011">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.30:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000008">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000004">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.670:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.199.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="entry name" value="Topoisomerase II, domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="entry name" value="Topoisomerase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050634">
        <property type="entry name" value="DNA_Topoisomerase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001154">
        <property type="entry name" value="TopoII_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031660">
        <property type="entry name" value="TOPRIM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034157">
        <property type="entry name" value="TOPRIM_TopoII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169:SF38">
        <property type="entry name" value="DNA TOPOISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169">
        <property type="entry name" value="DNA TOPOISOMERASE/GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16898">
        <property type="entry name" value="TOPRIM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01158">
        <property type="entry name" value="TOPISMRASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00418">
        <property type="entry name" value="TPI2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="Type II DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52040">
        <property type="entry name" value="TOPO_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15 19 20" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="16 19 20" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14 19 20" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="22">
        <location>
            <begin position="442"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IIA-type catalytic" evidence="23">
        <location>
            <begin position="691"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="410"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1062"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1167"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1296"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="412"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="1062"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="1198"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="1222"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="1392"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="19">
        <location>
            <position position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00019635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00053943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS52040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="CDF89603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1401" mass="160303" checksum="1B4335D1CAE7A26F" modified="2022-05-25" version="1">MTVDSKPASERYQKITQLEHILKRPDTYVGSVEVQESHQWIYDEETDSMVEKAVSIVPGLFKIFDEILVNAADNKVRDPSMKKIEVNIDPEANLIEVKNDGKGIPIEVHEKEKIYIPELIFGHLLTSSNYDDNEKKVTGGRNGFGAKLCNIFSMEFTVETADPNFGSKYLQKWENNMGLCHPPKITSYKKGPSYTKISFKPDLKRFKMETLDNDTLGVMRRRVYDVNGSVRDINVYLNGKSLKIKNFKNYVELYIKSLEKKKLSEGLPEMKSEGKGPAILYERVNDCWEIALTTSDMSFQQISFVNSIATTAGGTHVNYISDQIVRKITEMLKKQKKTAKSHQVKNLIFLFINCLIENPAFTSQTKEQLTTRVKDFGSQCEVSPEFVKKIMKSGLESKIMDIATENANNTLKKSDGTRKSRITDYPKLEDANKAGTREGHKCTLVLTEGDSALSLAVAGLAVVGRDYYGCYPLRGKMLNVREATVDQIQKNAEIQAIKKIMGLQHRKKYEDTKSLRYGHLMIMTDQDHDGSHIKGLIINFLDSSFPGLLNIPGFLVEFITPIVKVTITKPKKEIISFYNMPDYEKWRDEESHHYSWKQKYYKGLGTSSSQEARQYFSDLDRHLKKFHALQEGDQDLINLAFSKKKADDRKEWLRKYEPGTVLDPKLTEIPISDFINKELILFSLADNLRSIPNVLDGFKPGQRKVLYGSFKRNITSEIKIAQLANYVAEHTDYHHGPDSLAQTIIGLAQNFVGSNNIYVLNPIGSFGTRATGGKDAAAPRYISTQLNKLTRKIFHPFDDPLYNYLQEDENTVEPEWYLPILPMLLVNGGEGIGTGWSTNIPPFNPLDIVSNLRRLMNDEKLQEMHPWFRGWGGTIEKISSQRYRVFGRIEQTGPCTLEITELPARMWTSTIKEYLLLGLGGNDKTKAWINDMEEQHGNNIKFVIKLSQEEMNKTKAIGFYERFKLISSISLNNMVAFDAHGKIKKYESVAEILTEFYYVRLEYYQKRKDYMSERLRWELEKLSYQIKFIKLIIDHELIVTNRPRKMIVIDLEERGFPRFNKEGKPSYVKTSDEEKDKQEEQENEEEEENNAIINGPEEVIGSFEYLLGMKIWALTRERYEKLLNQKQEKQMDLETLLKYSAKDLWSLDLDEFVTAYDKFMADDEEARNGEIPGTASKTKNKRKRKTDQNDGEYNPTGKKPKVSKKTTAKKGKEDISGSFERIFLEPKAMTKDKRPTKVKTESQSPPSCSIEIAPKKKSEPTPASIKIEPREEEGISAFSDKFKKLAATFDDSEVTSNIADEPNAKPLAPLIPIESKSEAVNSKVKDKTNESIGGKQQAAPIENEEVPTRGRRKRPCRAAAIPKKSYQDNIILSDSSYDDQELKDESHSDNDFNLDEEDEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7F4</accession>
    <name>A0A8J2T7F4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA ligase</fullName>
            <ecNumber evidence="15">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">BN860_03598g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="15"/>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7F4"/>
    <dbReference type="OrthoDB" id="151490at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032807">
        <property type="term" value="C:DNA ligase IV complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07903">
        <property type="entry name" value="Adenylation_DNA_ligase_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07968">
        <property type="entry name" value="OBF_DNA_ligase_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044125">
        <property type="entry name" value="Adenylation_DNA_ligase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029710">
        <property type="entry name" value="LIG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45997">
        <property type="entry name" value="DNA LIGASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45997:SF1">
        <property type="entry name" value="DNA LIGASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 15" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="17">
        <location>
            <begin position="382"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="18">
        <location>
            <begin position="682"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="18">
        <location>
            <begin position="843"/>
            <end position="944"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CDF89409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="945" mass="108948" checksum="7500ED4079C90E86" modified="2022-05-25" version="1">MASIHVERSAMPYNFAPSPEFRWLCEELFVKLDNVQKQQRGRGAFKSTTVKYFEIISFFVKLWRKTVGDDIYPALTLILPYRDRRILNIKDFTLIKAICIYLKLPKNSPTERRLLRWKRRAGKGFKLSDFCVDEIRKRKSEPSHRDRITIEKLNECLDTLAVERSTKGRGFKGLSESASFKYCLENMTFTELKYYFDIILKSRVIAGQEHKFLSCWHPDARDYLSVVSDLKTVAEKLWNPTHRLRNDDLSINLGLPFAPYLAKRLHISYDKASSKLKNDFYIEEKMDGERIQLHVMNYGKQLKWFSRRGTDYTYLYGADVNSGTVAQYIHLDPKVKNCVLDGEMVSFDTNRNVVLPFGLVKSSARDALTPEGVLTKGYQPLLMVLDLLYLNGVSLVKAPLHIRKQYLSSILKPHPRAVEIIRSVRCFDDISIKNSLKKAIAMGSEGIILKQFKSKYHIGARTDQWIKIKPEYLEQFGENLDLIVIGRTPGKKDSLMCGLVVLEGDENLDNQPKKDIINLDSDEDEEPRKIAKKIVSFCTIANGISQEEFKEIERKTAGRWNKTESRSPPGFIEFGSKVPEEWIYPTDSIVLEIKARSLDNTESSGTKFKTGCTLYGAYCRRIRDDKIWTDCYTLSDLRQERKKNFPVRGNFTTQKSLRRKREHRIQGSSPLNQTLWCNDEFKTSHIFHNLSFYVMSDFMHSRGHIRITKEELCTIVLKNGGKLIYNVISRHGDLGNLRIVSGKYTLECKALINRGYDILSPQWILDCVSNNTIVKLEPRHCFSVSEKMQTTSKDRVDCFNDSYEATINEDRLSEIFQFTKEIPRCYFQPTEDIRGLGGIPLFLFSKRLVFVPDSFSYLTTETLKQKIKFYGGEVTFDIRKCNLIVIPDGGGALLEEKLNNIREILSTFATEGEVPTSIPRIVTTEWVNKSIEENCQVAEEGFLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7H9</accession>
    <name>A0A8J2T7H9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GPN-loop GTPase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_14312g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Small GTPase required for proper localization of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7H9"/>
    <dbReference type="OrthoDB" id="5839at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17871">
        <property type="entry name" value="GPN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000338">
        <property type="entry name" value="GPN-loop GTPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030231">
        <property type="entry name" value="Gpn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF3">
        <property type="entry name" value="GPN-LOOP GTPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF88612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="345" mass="39837" checksum="B41F8B5F1628009A" modified="2022-05-25" version="1">MPFAQVVIGPPGSGKSTYCHGCSQFFNAIGRHTAVVNMDPANDLLPYPCAVDIRDFVTLEEIMQEQQLGPNGGLMFAVESLDKSVDLFVLQIKSLVQEERAYVVFDCPGQVELFTHHSSLFRIFKRLEKELDMRLCVVNLIDCFYITSPSQYISVLLLALRSMLMLDLPQINVFSKIDLLKSYGELPFRLDYYTEVQELEYLEPYIEQERSQLGVNRFKKLTECISEMVTDFNLVSFEVLCVDDKESMIHLQNVIDKANGYIFGSSEIGGDTVWAEATRQGAMLSNVDIQQRWIDNKVYYDKIEQEKRESLLKEQKDQEKTFDVDDEWEQAIKDWEEKQGTDYVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7Q2</accession>
    <name>A0A8J2T7Q2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ZYBA0S04-06942g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_06942g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7Q2"/>
    <dbReference type="OrthoDB" id="1076608at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022257">
        <property type="entry name" value="PHM7_ext"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF26">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G10920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12621">
        <property type="entry name" value="PHM7_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="378"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="428"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="475"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="521"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="590"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="634"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="662"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="11">
        <location>
            <begin position="8"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="12">
        <location>
            <begin position="180"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="9">
        <location>
            <begin position="380"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="10TM putative phosphate transporter extracellular tail" evidence="10">
        <location>
            <begin position="794"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="879"/>
            <end position="911"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF89548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="911" mass="103390" checksum="B7C91EF4E856AA55" modified="2022-05-25" version="1">MATSTSQVLTAIASNSVLFGVFMTIFFLFRLKLKRMYEPKSSYDLIDEEKKPEPLPRGLWQWFVPLLKKSDNFVIQEAGIDGYFFLRYLLLICAYCGVSLLYMFPIILTVNATDGKNEGGMDRLAYQNIKHHKGRYYAHVFCGWVFYWMFLFVIYRELYYYNSLRCAVLSSPRYGRKLSSRTVLFQTVPEQYLSEREFNKLFDGVKRIWIARGGKQLLEKRVAQREELVHKLETTAVKHIRKALKAVCKELKKRPGEPVVPDINFYIRPEKRPKGRFSPLEEGPKSKFKFLNPKVDTMEHIRSVLPDLDAEITKLQMNNINASPFNSVFVEFESQYQAQVAAQVVTHHGPLSMCPSHVGIAPGDVYWPNMRMFWWERLVRSIIAVSAACALAIFWSFPVAFVGMISNINYLTNKLHWLRFIYKLPRPLLGLLTSLAPSIALAWLMSFLPTFIRMMAQIQGAATSQLLDYFVQQSFFAFQLVQVFLVVTVTSAVTSTVTKIVQNPTDAMTLLASNLPKSSNFFISYILLQGMGVSSAILAQFIPLIFFYIIGPRSDTTPRKKYTRFTDLDTPGWGTAYPVYTNLAVITFSYAIISPLILLFATVGFFLLYVAWLYTLTYVQAEGADNRGMNYPRALFQTIVGLYLGQICLLGLFAVGKGWGPIVLQAIGLGVTVIVHLHLNYAFDHLMKYVPVDTMKPLNGKSDTPSFKNIYKDTGTNPEEEIKELPKFPLPKYQRKNSLPPMDLPTSDSSDKTIQYNPLPEESYQLSENKISWVPLLADGADGHVPPAPLYKRFFLPHIYLSFKNVKNKLPETYGIPDPETPETEEANADAYNYPAVTSACPSVWLPRDPFGFSKQLVEEFCDVVNISDENATITLDGEIEWQGGPPAAGPPQSSKQGGDDDSSEEYKFET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7S9</accession>
    <name>A0A8J2T7S9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="16">ZYBA0S04-07998g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_07998g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7S9"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000016">
        <property type="entry name" value="Solute carrier family 25 member 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="430"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="14"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="83"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="202"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="323"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="430"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF89593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="523" mass="58865" checksum="A6F22FEC2D109D9F" modified="2022-05-25" version="1">MEAREGGEIETDEERSKRYEKLFRRLDMENKGQLDFEGLKEAFKKDNHPLRQNDDAMAVLFHAMDTDNDNVISFEDFKKYASMAESQIERGFQKLDLDHDGRIRLSEVSQYLAQLNSKDEETAFLNQSKSSLSNFVNWAFYRKPKSEEQASYITYDQWRDFLMLVPRKEGSRLHAAYSYFSFMEGDMDLSSEGDMTLINDFIHGFGFFMAGGISGVISRTCTAPLDRLKVFLIARTDLSSTFLNSKEKVLAHNPHANSSKIRSPIVKAIRTLYRQGGIRAFYVGNGLNAIKVFPESSMKFGSFEVTKKLMSRLEGTKGETENMSKLSTYIAGGLAGVVSQFSVYPIDTLKFRVQCAPLDDELKGNKLLFKTARDMYRAGGLRTFYRGVLVGVTGIFPYAALDLGTFSALKKWYIANQACKLSIPEDQVSISNLIVLPMGAFSGTVGATVVYPVNLLRTRLQAQGTYAHPSTYTGFRDVLSRTVKAEGIPGLFKGLVPNLLKVCPAVSISYLCYENLKKFMRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T861</accession>
    <name>A0A8J2T861_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="13">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_04940g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="13"/>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02735">
        <property type="entry name" value="RNAP_I_Rpa1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01435">
        <property type="entry name" value="RNAP_I_RPA1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.30:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.100:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.120:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.180:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2850:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2850">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047107">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015699">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF11">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="15">
        <location>
            <begin position="351"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="276"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1351"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="300"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1398"/>
            <end position="1415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1416"/>
            <end position="1425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF90606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1661" mass="185836" checksum="CA99037A061C503A" modified="2022-05-25" version="1">MDISKPVGSEISSVDFNVFSAEEIRNLSAKQITNPTVLDNLGHPISGGLYDLALGAFLRNLCSTCGLDEKFCPGHQGHIELPVPCYNPLFFNQLYIYLRSSCLYCHRFRLRAVEINRFACKLKLLQYGLVDESYQVDQLRVGDLNAESDDEGDEAIEDNTHESDSKKALKDVSAAMLKELIQKREEFVDVAIAKAISEGRSSHKGIFTIITNEERKKLIHEFHRKLLSRVKCDNCGMFSPKFRKDGFTKIFETALTDKQLTNNRVKGVVRQDMIKKQQQAQKLGGQENENGVDEDFSLGKNPSSRPKTGSTYILSTEVRNILRVLFKKEQEVLQCVFHSRPNTSKGPVSADAFFMEVVLVPPTRFRLPSKLGEEVHENTQNQLLSKILTTSLLIRDLNDEMSNLQKDKVSVEDRRVIFSRLMNAFVTIQNDVNAFIDSSKAQGNTGGKIPIPGVKQALEKKEGLFRKHMMGKRVNYAARSVISPDPNIETNEIGVPPVFAKKLTYPEPVTAYNIAELRQAVINGPDKWPGATQIQNEDGSLVSLIGMTLEQRNALANQLLTPSSNSATHTLNKKVFRHIQNRDIVIMNRQPTLHKASMMGHKVRVLPGEKTLRLHYANTGAYNADFDGDEMNMHFPQNENARSEAFNLANTDSQYLTPTSGSPLRGLIQDHISAGVWLTSKDSFFTREQYQQYIYGCIRPEDGHSTRPKLITVPPAIVKPTPLWTGKQIITTVILNITPPNMPGINLLSKNKIKSEYWGKGSTENEVIFKDGALLCGILDKSQYGASKYGIVHSLHEVYGPATAAKVLSVLGRLFTNYIMSTAFTCGMDDLRLTDEGNRERSEILKTSADVGRQAATEVTNLPKDTSADDPELLKRLEEILRDDNKSGILDAVTSSEVNTITSKVVSAVIPKGTMKKFPQNSMQAMALSGAKGSNVNVSQIMCLLGQQALEGRRVPVMVSGKTLPSFKPFETDSMAGGYIKGRFYSGIKPQEYYFHCMAGREGLIDTAVKTSRSGYLQRCLTKQLEGVHVSYDNTVRDGDGTLVQFLYGGDAVDTTKESHMTQFDFCLDNYDALLKKYNPSALVEHLDVETALKYSKKALKNRKKLGKEPHYKQSLKYDPVLTKYNPAKYFGSVSEKFQDKLEDFLESHTKQLKSHLNVNEKKFRALMQLKYMRSLVNPGEAVGIVASQSVGEPSTQMTLNTFHFAGHGAANVTLGIPRMREIIMTASAAIKTPQMTLPVWKEVSDEQADTFCKSISKVLLSEVVDKVTVTETTGTSEHGSPARSYAVHMRFYGKDAYGEEYDVSKEELQVVVATKFLYLLEAAIVKELKKQKKTSMPDIGVAIPKSQTALGTGEGAVSGSAEDNDEEEARKKTKQAVSYEEPDEDEIETMRQAEKSSDEDELDSDMESSPESEVDSDKEMEDAHEVDASEYTKNMSKSQRDRQHAVISEHKFITKYNFDDEDGKWCDFKFELSGDTEKLLMVNIVEDICRKSVIREVPRISRCVHGDSVNDRRMLVTEGVNFQAMWDQEMFIDVDGIRSNDVAAVLRTYGVEAARNTIVNEINNVFSRYAIRVSFRHLDLIADLMTRQGNYLAFNRQGMETATSSFMKMSYETTCQFLTKAVLDNDRERLQSPSARIVLGKLNNVGTGSFDLLTKIPNTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T893</accession>
    <name>A0A8J2T893_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_06612g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPL family. K-HECT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="423283at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045322">
        <property type="entry name" value="HECTD1/TRIP12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057948">
        <property type="entry name" value="TPR_TRIP12_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25579">
        <property type="entry name" value="TPR_TRIP12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="8">
        <location>
            <begin position="1109"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="1413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF90002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1446" mass="162275" checksum="031DF0044FF791C8" modified="2022-05-25" version="1">MDENDHPSGSSDYMVDEQSSADGQDEYEYPDATSSERGYDESSGQFHDAQDEEQDMFIGYDGAEEGDEDHAVEDENSSTTSFTVQDILGNLAQTMEGHSQGVERSGPFANNRETGPMNLAGVFPEILSILNDGMQQRGSGGGRNDRISKLVKNVLNADEDPYIAMESLRELSEHLLMTNQVVVDRIFPIEKLLSGILNVLSNPMLKGELELQLISCRCLYNLFEVNPKSITMAVEENAVHVLQEIFQEISYIDLAEQVLETLELISRLHGREVLKSGALASCLQYLDFFTIHAQRKAVSIVANSCTRVRMESFGIVQTLFVLLKPIFVNATDQVIISRLLNSLYGVCGGLREADMLESLFTFEVVERLLQLVSIPDTPLEDRIKCLDILSVLLNVSGKISKEMLVTCDLTRTFSDCLECYGKATNSALHETLMFVPKPLLHAIARTVALLFPTETEQLLSVDPPKEIDLSNNSEKMSKTLTNFAILLIEIFVNTVEFDVRRCVLIALARIVPFLKRSMTGQLGSHIIRLVGSTLAQNEAALNRENEQIIVSGGLLIGVLSLLDILTARFASDFLPAIRREGIVDLVDRLYGILNRMKDEGKFVIEECNVSLLAGDGGSVSESGEVMEEEEDDDDEEYGAQFGDIDIPDQVKPKQIKFEVLRPLNSNYIYMKAYDLSKNLTQLFSHNEGVVIEELKEIEKLVLDLNELDAKDFSEESWSRLWTRVRDNIFNGNFEISSFELISSGLAAKLSQLVDHYSCSSFIPERTFIRVFGDHLSKLVEILQSALTRLESFHIMECGLQGEEGGVASLGKQMKIRLIYDGDAQEDSIAPHLTTITLSIHCVASLKTLSEFLKHRIAQTRFLNSLLPGLTFSSANTSQNNADDLNHWEFEFSTEEGVLNPSDTIFGAVFKSVRASHKELSEIWRTVQVIKFKRVNDQNISEEPKLFNLYTQHNFGKDVLKPAEDILSLLKFARASDLPSDLFVNAKLSAKLSRQLEEPLIVAGGVLPDWSLYLTKNCPFLFPFETRIFFLQCTSFGYGRLIQIWRKHMGKGKETMADDPLQQLGRVTRHKLRISRNTMFLSGLKILNKYGSSPSVLEIEYQDEVGTGLGPTLEFYATMSQGFSKKSLGMWRYDAYGSGQLDAEGNSPYVNGLLFPASLTSTQNEVKILELFHYLGTFVARSMLDTRILDLNINKIFFELAHRICNRHGVPNKEMDEQDMLRLLSFVDPQLARSLRALYTEADLESLTLNFTLPGYDIELVHEGKSLLVSSNNLNQYVKRVLDYTLGTGIEKQLTSFIDGFSTVFPYSSLLLLTPEELVHMCGRTMEDWSSETLYASLVADHGYSMDSSTIHDLIYVMSTFNDREKRLFLQFLTGSPKLPIGGFKALNPKLTVVLKHPEDGLQADEYLPSVMTCANYFKLPKYSSQTVMRSRILQAMQEGSGAFLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8H8</accession>
    <name>A0A8J2T8H8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_05270g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in vesicular protein sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the VPS35 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T8H8"/>
    <dbReference type="OrthoDB" id="10258141at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030906">
        <property type="term" value="C:retromer, cargo-selective complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.660:FF:000007">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.660">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35, helical subcomplex Vps35-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005378">
        <property type="entry name" value="Vps35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042491">
        <property type="entry name" value="Vps35_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03635">
        <property type="entry name" value="Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009375">
        <property type="entry name" value="Retromer_Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF90296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="884" mass="102610" checksum="480A68D316D320F0" modified="2022-05-25" version="1">MSYSETLEQALTVVKQQAALMQRCLQQRKLMDALKHASIMLTELRKPDLSPKQYYELYIMVFDSLSLLSSYLTENHPTSHHLADLYELVQYAGNVVPRLYLMTTVGTSYLKCADSPREEILKDMIEMCRGVQNPTRGLFLRYYLSQRTKQLLPENSLEFNANFVITNFIEMNKLWVRLQHQGPLREREQRTKERKELQILVGSQLVRLSQILDDNLEIYKDRILPVVLEQVVQCRDVVSQEYLLEVVCQVFPDEFHLATLDSLLEATLHMNPDVSINKNALTLIERLNGYIDRKEQEHEKPQAGSDLFRVFWNYLKTLNEERPDLSLQQFVPLIESIMALSLRWYPEKLQNLNTLYGFTAQKCRDYGKAIPPSAEYLFVNLLTLQNFEHVNCSAVLFYNIISQCDSFRELLSLQNVKLQKHIIDNILDTFLTPQVDFEKTFTIDSKTNLEKLLSVMEPLIRLRSPISGKRPSTGALVLGGEIDEEEGSGSWILDPSQEKLAKFCHVSLFLVPTKSKQFNNIETQAEYLLLLKNWYYKGGKNIKYTYPAIISNFWKLIRKAFLLKTRQRLQKATEQNYSNLIKQLFKYASRCINDLFNVCGSSVIDTVYKLNIQSAALADQLSLGEIAYDFFSQAFTVLEESLSDLKTQFQALVYMIQSLQKTRSLYQESYYDSLIVRCTLHGSRLLRKQDQCRAVYLCSHLWWATEIALIGEEEGITADFYREGKRVLECLQRSLRVADSTMDNIQSCELMVEILNRCLYYFIHGDESDTHVGVKYINGLIELIKTNLKSLRMEEQVPEQLRVQDLHIKDQESSEATKKRFVIGLDGTYIEVPKTNGTPASVCNKLNSIYINEMIRIPIEHFDRTCSYIVNQREIDDRFKIIVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8R4</accession>
    <name>A0A8J2T8R4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ZYBA0S09-00804g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_00804g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the patched family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316462">
        <property type="protein sequence ID" value="CDF90923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T8R4"/>
    <dbReference type="OrthoDB" id="6510177at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015918">
        <property type="term" value="P:sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1640.10:FF:000029">
        <property type="entry name" value="Putative Patched sphingolipid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053956">
        <property type="entry name" value="NPC1_MLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032190">
        <property type="entry name" value="NPC1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727:SF2">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22314">
        <property type="entry name" value="NPC1_MLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16414">
        <property type="entry name" value="NPC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035197123" evidence="12">
        <location>
            <begin position="22"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="556"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="583"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="613"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="665"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="690"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1001"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1027"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1052"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1093"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1132"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="13">
        <location>
            <begin position="555"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF90923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1177" mass="132216" checksum="37FFA9FC8A72B637" modified="2022-05-25" version="1">MLLSTLWITSISLVNVAGATAQCAIYGNCGKKSIFGQELPCPVNSTFEPEPASDDLKELLVGVCGEEWKEIDTLCCTKDQVLSLKKNLKKAQNIIASCPACMKNFNNLFCHFTCSPEQRNFVNVTRTQQSMDHREIVAEVDVFTNASWASLFYDSCKDVKFSATNGYAMDLIGGGAKNYTEFLKFLGDEKPLLGGSPFQINYLYETQDSFKLFNETGYPCNDPVYKCACADCDASCPELGRLRKGSCTVGKLNCFSFSVLIVYAALLLGVILLHIYLFLFKKKTSLISNDDNTSAENSGMTSGDRLFETYDVKSYRINNFISYVAGGVSRYVANNPYFVLAFSAGLVAVSGLLLAIFGELETDPINLWVNQNDEKFKEKMYFDEKFGEFYRVEQIFVVNETGPVMSYDTMKWWFKAERDITELLRNSEGITYQDLCFRPSENSTCVIESYTQYFQGDLPVKSTWKGQLKACTDSPVNCLPTFQQPLPKNMLFSDDNVLQSKAFVVTLLVDNHTEEAELWEQELEEYLLHLDIPEGLRISFNTDMSLEKELNINNDIWIICASYFVMFVYASWALKKRNGESRWFLGFAGILIVASSVICAAGVLSTLGLKSTLIIAEVIPFLILAIGVDNIFLITHEFDRITEEQYAKRIDERIVKAVQRISPSIFASLLCQAGCFLIAAFVSMPAVHNFALYSALSVGFNVLLQLTAYIAILVLYEKEYGVAPHLPLDEEKKSAIFGERYFNFISKKMKVLSLFVTYTLVSLVFIPEIKFGLDQKLAVPQDSYLVDYFQDAYQYLNVGPPVYFVVKDLDVTKRENQQKLCGKFTTCDTKSLNNVLEQERKRSTVTGPVANWLDDFLMFLDPQLDQCCRFKKGTHEVCPPNFPSKRCETCYEEGDWQYDMSGMPEGKDFLEFMEIWINTPSEPCPLGGKAPYSNAIAYNGKKIEASSFRSSHRPLTSQKDFILAYDDAIRITKSMKGLNVFAYSPFYIFFVQYKSLLSSTLVLLASALALIYVVSAVLLGSIVSASLLTFTVLMILVDIGAFMAWFNISLNAVSLVNLVICVGLAVEFCIHIVRAFTMLPHGTKNDRDSRVRYAMVTIGDSVFKGITMTKFIGVCVLAFAKSKIFQVFYFRMWFALIVFASAHALVFLPILLSLAGGKSYVDDSADSETSDDSARES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T996</accession>
    <name>A0A8J2T996_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ZYBA0S07-05732g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_05732g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T996"/>
    <dbReference type="OrthoDB" id="5069333at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF29">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00755">
        <property type="entry name" value="AFLATOXINBRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="44"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="110"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF90640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="969" mass="109809" checksum="F594132EDC5E38EB" modified="2022-05-25" version="1">MDPLKQTPNTGFFLGDLFPSGGSPSEVSNEIEANVKKRNRISFVCQACRKSKTKCDREKPRCGRCIQHGIACVYDVKRQRAPKNPSKDAKIARLEKEVDYWRRKAMTSMQAEVRPTMQALPEEKGQEGREDEEDIPGAPPLKASRPSTSSNYNDGADEVLINLYKSHPSMIRNGACKREVKPLSENYVITQDMYLSGLLTSIFADKHTTIPGLSTNASVSRLKDVLMGQGRPSSQVRKLDELTDNTADNGGPNNAAMAKVGFLLQSVFERHYLEDFCPRHGEYSDILKSFIKEMEDILPPYDVIQEYKVHFYRSVYPSVSFLDEKTFEDALQSTLFPDQLDPSKVVIRLGNSRLRHKLENLCILLLVLKLSYVSLQVVGKGSQILTFEQKENLERYPIGNDAIMLVQQVLAAENFFSRANENIIACLLYIWAFFVYSPEESDFFQEQPTDLIIGLAMMLAMSIGLHRDPSDFPHFRDSSLVDRSILNYRRKLWIATVTCVCAETSLKGRLPHSSSDLMRLFINIRAPNALQVYMQKVTRDACGPPSPQYLQNHENCFKRAQLALLILDLHKLTLTYNRSFTLGAIEELRAKIVKFLDENFPMQDFPTDPENGYHEFERYINSTALHMRIIGRLMILRSSMAIFLHLESLLTEGSKLLPYYRQYFNQICLDALNLITHFRAFFISTRKFKALPLGSFNIAKYIQLALPSTLLAVLAILMRIDLACNMLSQSENRSSGSTSIPSEKLDMMILLKNLLKSILEDVHKAASEHLRFSYFSVFKTLSLFDVIIAKIRKNELWTCVMKLQKLCEVDKPFLKSLNTNLSFLCLDPNNQESIVEKLKQKNYVTSFSSGELDAVYNGIKTIYTRLPQISTPWANESSPKVPLRTLFQTFEDNDNNAGFDRTASLAALDQNINLTAPPDEKIKTTNQEVVQDGTSNVVVSEEGGPTIAEEFPGSFGGLDIFDFLFGNEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9J5</accession>
    <name>A0A8J2T9J5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">5'-3' exoribonuclease</fullName>
            <ecNumber evidence="14">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN860_01882g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Possesses 5'-&gt;3' exoribonuclease activity. May promote termination of transcription by RNA polymerase II.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Possesses 5'-&gt;3' exoribonuclease activity. Required for the processing of nuclear mRNA and rRNA precursors. May promote the termination of transcription by RNA polymerase II. Essential for vegetative cell growth and chromosome segregation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with RAI1; the interaction is direct, stabilizes RAT1 protein structure and may stimulate its exoribonuclease activity. The interaction also stimulates RAI1 pyrophosphohydrolase activity, probably by recruiting it to mRNA substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the 5'-3' exonuclease family. XRN2/RAT1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="14"/>
    <dbReference type="EMBL" id="HG316461">
        <property type="protein sequence ID" value="CDF90740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="28245at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18673">
        <property type="entry name" value="PIN_XRN1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1050:FF:000002">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000003">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000005">
        <property type="entry name" value="5'-3' exoribonuclease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017151">
        <property type="entry name" value="Xrn2/3/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF41">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037239">
        <property type="entry name" value="Exonuclease_Xrn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 14" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 14" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="Xrn1 N-terminal" evidence="16">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="17">
        <location>
            <begin position="314"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="17">
        <location>
            <begin position="494"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="519"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="570"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="943"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="520"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="570"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="581"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="952"/>
            <end position="983"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR037239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF17846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CDF90740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1033" mass="117851" checksum="971D80AB072EC9BB" modified="2022-05-25" version="1">MGVPSFFRWLSRKYPKIISPVLEEQPQVIDGVTLPIDYSAPNPNGELDNLYLDMNGIVHPCSHPENKPPPETEDEMLLAVFEYTNRVLNMARPRKVLVIAVDGVAPRAKMNQQRARRFRSAKDAQIENEARERIMEEREQLGEIIDGAMKQKKTWDSNAITPGTPFMDKLAAALRYWCSFKLATDPGWKNLQVIISDATVPGEGEHKIMNFVRSQRADPQYNPNTTHCIYGLDADLIFLGLATHEPHFKVLREDVFAQGNKKRHNIKDTMNMTEEEKQLLAQQDSEKPFLWLHISVLREYLSAELWVPRLPFAFDLERAIDDWVFMCFFCGNDFLPHLPCLDVRENSIDILLDIWKVVLPTLKTYITCDGELNLESVEKLLSQLGSREADIFKTRHIQEVRKQEAAQRRKLQKNMSKGQDRHPTKPTEQLQLYDTEGQLAKGSWNLTTHDMVTLKREIMLANEGDQKAIAIVKAQSDKNDSLMNDIMKEQMDKVVDEANRTNFTAADVMKKKLAAKKRRLEAEEQERASEQQSEANKKIKTDINKPEENSEQSNLDQEIEAEIKDEVEENALEDEEQQESEEGKPADRVPEVSSGGITSGVVDTEQEVKLYEPGYHERYYTEKFHISPKDINALRKDLVRCYIEGVSWVLLYYYQGCASWTWYYPYHYAPLAADFFELADNKYEFSLGEPFLPYEQLMSVLPAASGHTLPPIFRPLMSSPDSEIIDFYPTDFPIDMNGKKMSWQGIALLPFIDEERLLRVVRAQYPKLTDAERSRNVRRNPVLLISNKNANYEKFLKKLYSSDAETPELHFQHFRSGLSGIVTADHEGYRPNSKLSCPIQGGSLPELSTNLFLKVEYRLIPLPCQNKSVILNGFIPSESVLSPYDLDAVMYKYNNGGPRRWNFENDMRNNVVPVGPCGITQYKPRVGGYRSFFFFGSTQPGMGPPGGPPVGQPGAQAAGARYQSNNGSRYSGSSRYSGAPAGGRHQNGPGGSRYQGGRPQRSGTLRRFXKSFFFFFFFFFSYLADVGERANVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9U8</accession>
    <name>A0A8J2T9U8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ZYBA0S09-00518g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_00518g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BLM10 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316462">
        <property type="protein sequence ID" value="CDF90911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="17907at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016504">
        <property type="term" value="F:peptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032430">
        <property type="entry name" value="Blm10_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032372">
        <property type="entry name" value="Blm10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035309">
        <property type="entry name" value="PSME4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021843">
        <property type="entry name" value="PSME4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32170">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32170:SF3">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16547">
        <property type="entry name" value="BLM10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16507">
        <property type="entry name" value="HEAT_PSME4_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11919">
        <property type="entry name" value="PSME4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Proteasome activator Blm10 N-terminal" evidence="8">
        <location>
            <begin position="75"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator Blm10 middle HEAT repeats region" evidence="7">
        <location>
            <begin position="507"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator complex subunit 4 C-terminal" evidence="6">
        <location>
            <begin position="2056"/>
            <end position="2143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="24"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF90911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2143" mass="246823" checksum="8DC6F4B58AB4998A" modified="2022-05-25" version="1">MTGNDITAPIPLKNRTLSQLKVEEMKNTRSSSSMAGDGTKRPKLLSSDIGQERRPRSVTPSASSPQLINGMDSKQIFTERLKHYGLDYVDDRMEHLRNIYDPESKWFSRAVKPPCLLEDTLPYKTESHKDQAKYLCHVLINLYIAIRSMDIQGLISISSKDLADLKDEVDNLALNTDLFRLSCENVDADMLNNDIADFDEDEDEEDMFDEYNEYIDLIGPDFNATGKITAKSASIINVNHWTNEFKNCMHFDFPLSLRKSLSVVYYYLSLVQGQKVYRQMHVEMFESLVAKDDDGTNFTDLLFDAGLRLDHTVMLRFLADFLPYPEPDYIRYDLIIKEDLQLFRLLLKLAHNSRAFYEDKSSSILFDSMEYLLSSLAPSTISTIMPMITSFVPYQYHDQCRITDFFPFCFGFWCCKLADASVDTHMYDFMGTVAEDAHWKLIRHKQPNGLVKYGNFLLFTDEQISFMFNRLQVHLRADGQIHSYSRTVRPLIYALNGSHSDAFFERLENLTKSIETFVHPSNSGFWTKPISKLVHGFIKMYHGRVQKEKKQKELGIQHPICVDSQCNSKLVDTFLGLLLLGAQNKSSDVANYYISCFAYLLDLWPENSHVIFDRILLDIYEALAGEYVNSTHRLTASLKQFNRVVRYMVMDKVYRVHVTNILLMLVNKIDLNDIGLTSNLINGIVSIASFVPFQNFVSEDEHLTFESHTLPFIEQHYYYFRDPSQQTGFAYDEGILETAFKASTTVFENVLRVYIDKLFQLADVDLEEGFVTKVNQTTMIMQESMDDKTFKIFAKLLQKKFWDNDAFKGKDPQYDLVIIPLAALIRRDNHLAQPLFEGLTYHIREQIDRGAGSVRASSEIQQRDVKLVLYLSALNEVLRQSRNAILGFSGNFKEFMTYVFESVTNPPLDVITSIMIHSACASLTSTEITDYRLFPEQSEIPLNERWGGLQFDKRKYDKRNLDFTWHVPNSAEITLAIDLLRTVTDYCVSNVENMLAEPHTDFTYSDKIQKYILIITHALSGSSLLFDPDFNKNNSQTTSDWQYYKEKLILLKNVRENNFDSQELDIDIEQIRSEKGSEEYIDEKDSDRQVSEDSGVVEMKEATSEEIFMDEDATMSELPSGIATPEPGSHLNAGLNSSMNSRLAFRDLDIYTCNYYFGNTAAEKLKHSQYLEVHQMRADIGKFLHNLHKFLCDHFENNTSMFQILLHGMKVWFTDVGQETVFIEDPCAFFDLDFIENIQLLGNAVEPLTRTCLAARANDLHQMRVLLHSTNRYPSKLETHLLRNIINLAFSVYPDIHKPAQGTLIHCMKQLIGSYSIIIRKAIIALKEVIQSRDYMKMQVILKLLMIKKIHRKLMSDYNNIADLTMLLIECCKVNELEIAMYADKILNDIVAGLKIPSSICILDERAYTPLAPPDSFMDLQVEAVKHAKDKKRGCYLSLLVDLQRKLIDILENESTIGWKLPMIIIRFITKVQSHLEIQVDTDAVETIFNRSKTNHPDMIHLAIKSFLGVFNKIFSLADYDYDVARAYNSAFDPDFIKEVNTLQDDFHTKFRREMNNFESPRYFVDSRMFVGWLCWGRSMKVVDSSPIEMSLRPNEVEAFEKFGNLVSYDWLQSMVSNLIQDNETQGVFSSGNVSFFVLLIYLISHNFTKLAFEDLFQLCEKHYDRNDKASMILSTEIFAALICGSKYMYAEDIKTRDKFMTRFLPSCINNELNQDAFGIWATLCWWLPTVVDLRRCAPFFYQFSNIRNLLDKKSDDPAHQASKLLMLRSVLLSMEYRAPNLSSVVKNLVTDHPYDQVREAVAKVLTTLMQSSSCASWPNVQALMAKQSSPADGLGSPVMTTSEELDAFVKKQFADVEEESRKIAGLTPQKVLKTRYYYLTSTLIYWIKEMIKGTNRVLLVPYIVEYVAPFLLNLLTHRDVCKLAGIDPTVLYITLSYMPVRSDTVSKVVSLMCLERALHSSYQIKLKLGFVQHFFSSQLLQLTADQRDVILDFVVSHLYNEKYVEVRTRAADVLSDIVHNLGEHDKRLERLVQKFSQQLGRHTWEEKQKLSKADVRIHGSILGLGAIIQAFPYVFPLPRWIPQQLSTLASWSRTSGVAGTAAKTFISDFKKVRTDTWKFDRLSFTQEELEDLEGVLWSSYYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TBC8</accession>
    <name>A0A8J2TBC8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ZYBA0S13-02432g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_02432g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316466">
        <property type="protein sequence ID" value="CDF91738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="27109at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13315">
        <property type="entry name" value="PH_Sec3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028258">
        <property type="entry name" value="Sec3-PIP2_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048628">
        <property type="entry name" value="Sec3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019160">
        <property type="entry name" value="Sec3_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092:SF14">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092">
        <property type="entry name" value="SEC3/SYNTAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15277">
        <property type="entry name" value="Sec3-PIP2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20654">
        <property type="entry name" value="Sec3_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09763">
        <property type="entry name" value="Sec3_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01313">
        <property type="entry name" value="Sec3-PIP2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec3 PIP2-binding N-terminal" evidence="6">
        <location>
            <begin position="134"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="261"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="459"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="628"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="672"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="265"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="299"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="314"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="350"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="416"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="460"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="488"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="523"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="628"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="672"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="725"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF91738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1454" mass="164235" checksum="54A87DD2BE87E148" modified="2022-05-25" version="1">MIKSAKSPFKKKAHSREGSIEDRGFFHRRSTSGSNIPVAQHSRQASNGALNMGPPVSGVNSSHKRTASNSSRSSQSSNFLAEQYERDRKAILASCFSASDNKTSSPPNNYITHVRIIEDSRYPSSRPALDSKLENKKKRILILSSKANNPKDVQLHKGRENQDGRFQIGRTWSLKELSAIERDVEVNEGFVLIMAKRYYWETNSAKERVVFIKSLVNTFMQAYDGHVPELINWDLSIFYLDERTYQRAVMRKSVSFARPTFIPPDSSNTSTNQNVSPNPNVRLTNLDTSSLVSQGQMFPSPRSPTRTPVRSPSLNDQFFQNRQDTSQPPSMKSGYSNSPTLSSEGHSFYDQHYQQQQQQRQQRQGQQGRAWQSQRQQDQEQSPQQAQQAQQARQAHQEWKAHQAQKEQQSRRDQQMRQAQQARQAQQAQQAQQEHDRQQEILRLEQQREQLAWQAQLEQNAPQEAASGNDSALHQPLRGDSNDFMPLSQKTSRTSGRSARPSPDVAASPYFNSPHQFPAKQGTPDTSSTATGQFSVKQSTPELAHMRQTPVKQASVVPFDADDSMAEPMNAENPQLQQDHLLEDLNNVLGGVPSNTTFSDNHPIVAEESPKIPFGNAAFKKELSPVNTAPLSLPQTEPLSTRSVPHESTMVDVSDLAEDLNVSSIDVNHEDSTDLSFEKGDEVPLNQPLPPSSPHVYHEVTTIQEEAPPIDGHKRSLAPENTTEQNRDEPKSKGAIESADIEDEELLGILTDINWELGDAADDLVEKLNMKLAETTYSLNNGLLSLENLGPSLLPYEKQVDEACDKMNPIFSLFLMEMGNVAEDIEYIENQKNGLQIESANKKNLWSTLSELMNTVSLDDATLNELLNSPVSEKTLVRMETQLRSLFRALKAISGENDEEDYNLGEMQALKERRETYERVTKVFLERLVNALSGMFVNIYKDNTTEDHLTSFLSRLLIFSSLTLFCKDVDSDSYYIIVDKWVTSIQTVYNRICEKMISNLRRHKLDRREASTTANDKGLDNLMVQWESFKKTRTIPEIVSSSAKAVSHLKNSLESLGKLCICYQNFIDSFFHISSTLDFEEYLEQYTDPNTRTIALYSVKPMQSDRESALKEAQLVSRIFRPNVAKITSYFSSVLKADSSKAPAIMFFLEEKLQSLASSNQEFLLTAMSQILDQVKQLWSEHIEDEVVRLERSEINMSSRSISPSVIDFALFVRNSYEFVSYAQSHSQVKSTQSFESLKYFESDSGKLGSAITKLLSEKEGAAGMLSDVRGVGSEVTDIDRIITLLLNSYWLFEILSMLNVRGSFDPTIQAAKSIFDSQKEAYAAYLLRDAMPKLTSFIHGASSLVGTSSSGGSNLSKWAAYSRQNLDNILAGYTSSEIDILVKRLYQHMMRHLSYELNDLMKGTLCDKLWSCIQGQTVSLYLKLYTLIEKHYKGAFVRFTKNDIITAFERYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TBF5</accession>
    <name>A0A8J2TBF5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Topoisomerase 1-associated factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_01002g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the timeless family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316467">
        <property type="protein sequence ID" value="CDF91783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2TBF5"/>
    <dbReference type="OrthoDB" id="310853at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031298">
        <property type="term" value="C:replication fork protection complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044998">
        <property type="entry name" value="Timeless"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006906">
        <property type="entry name" value="Timeless_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940:SF4">
        <property type="entry name" value="PROTEIN TIMELESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940">
        <property type="entry name" value="TIMEOUT/TIMELESS-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04821">
        <property type="entry name" value="TIMELESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0236">DNA replication inhibitor</keyword>
    <keyword evidence="8" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Timeless N-terminal" evidence="11">
        <location>
            <begin position="36"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="919"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="968"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1118"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="968"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="978"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1118"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1170"/>
            <end position="1179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF91783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1199" mass="137958" checksum="55F6CEC0BBD9364B" modified="2022-05-25" version="1">MTDSDDISTTVLKARIALLATAIGGPDYNSRLDPPPYKLGDDCLACLKDLKRWFRLVDENQNRWDVAMATAEYRILQDDLLPILIDWENKSYLAHKLNKQSVNKDYHDNVALLCVQLLVLMTWPLVLTDQSTAKQVVVYSELKKHQLVYKKHLLMMEKGKVLKAASRLALKIIQMDKIDRNPRDNVILRLILNLFRNIVAIEPGELNVTRRKQLSSKGINSVDMLPPNVSMDDISLNAVITAFHRNKVFGLILTLSSSLSSEFEQDYINLPLMELMFYLTKDIAPGTLCIEKHEKREKNKVSDTGAELLNLLNKENTLKKNVIKNTSSRHSRFGALLSIQTPDSGRLTISGGGQNLLDDTIALKKLDNGKKWNKRSMQARDDVVVEGLPNSLLNTVARTNLFQESTMLTFTKFIDAFISSSFNPLLYSVTNHFTTDHDQMSTLEQTEYLLFFAWFVKYQREKCVKDIAADITCVDHALRETSLILMSTLLRTGYERKNWAVAHAGMIAFNELLLLLSYMRDHENSEEIEFILTKIFSEERIQLLTSLPKSAFRHSLPYMKSCVELVHTILRMLEPYNNGESLMIAKKNRRRKSKKISETDIMEMMNSHGIGRDEALDIFNPSFQTTEFNFMKILRKYCVESVVDTYINYLQRFKELEEGEIKKSMVFLHRVFVQASEESMLFRIDLLVLLRDMLAPDGISRKARVRVHVEEFSNYFLHKLKKRLKHSPSWFVGVLFPLIYGSEVGYFQKYGEKKPRKADQYFAALPSTFKHIEDEEQLPHSVLNDMKYGILISTLIDDGKNELLDQLMAHLQLTLDHFRSWLTVNIQQERETQNPPDEIFKIVSDNELQPLLFDKDFRALLDIIGFHLPLHADDLCKLPGDIEISSLEESLELVKKYISTPFQTPNGLPSSSYLIRPRTNASVDEEEWQTGDANDHHSPDISSQSPRLFVVDDDLYFEELGNDMTDRVSGKRIERGLAKSKNKKKTKTGTSNKKRNNSTLPKHSIDEPVEKTKHRKNVLSKDFISDSENEEDYINPLFFENEMYMRWLLDKYRGQLPEDKYALFGKFSSERIANDGLIKNDYSALFGGSIPDLQVLQHSETSANLPDKTLLSLSRRVAAEMEKESEENHKGADNTDSEFENFGNRSPRKTFVEDGTSSPLGKKQVTDPSSFEEEDSEETDLPRKKRKVFFDYDEDSEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TD36</accession>
    <name>A0A8J2TD36_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ZYBA0S12-01596g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_01596g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316465">
        <property type="protein sequence ID" value="CDF91555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="296386at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF73">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="488"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="518"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="572"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="608"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="654"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="714"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="800"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="911"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="7">
        <location>
            <begin position="480"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="64"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="340"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="980"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1065"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1381"/>
            <end position="1746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="66"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="230"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="343"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="986"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="997"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1087"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1117"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1162"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1175"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1197"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1252"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1269"/>
            <end position="1283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1289"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1312"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1436"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1511"/>
            <end position="1521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1543"/>
            <end position="1557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1617"/>
            <end position="1632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1642"/>
            <end position="1663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1672"/>
            <end position="1697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1731"/>
            <end position="1746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF91555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1746" mass="187121" checksum="2084F46DACA5D51D" modified="2022-05-25" version="1">MSDNLEQLDPNYVVAFDYSKGNVAQLVSEFAANGLAVVTRPGHNSKTVYAFTRIEEPSVFESGEVFTEKHGKKKEQEQQHHQHQRGGAVNEKLLGRKPLEPAQQPASIRSIAPESLKQSPGAVDSHVSEKSGPAETGGASDNRLEAAKSKMVAGEEAEASIQGRIPGSGKLGGLAGVGAAVTAEEGSKNGLLGHAKGGLTGIKKDELIKDGKQVLNDNKDGLADAGQKSAKGDKSQLESQGKKIATDAADKLDKDHVMDTGKKLLSGDKKDIIKNAKKAVVKNQLEHQGKDLLSGNGIGGAAAAAEGLEKKAGSGNSGEKTDHPEEVALDTGKEYLAEKYGTSGVNRTESANKAANHADKSYKRKVATDTETVPEFGNESCKAAVTSGVNATADTNAIESEKSAFTSANAQELTGGGRATLFAVVASLDFVQSVTPIYDIEKRKKLESTVNSLVNTVSLTPSDRDLVNLEHLTRNPREILYFFYFKNYIRWLLPISLVGVVCRLFSRSVAPCEFNITYTVLLIVWSLLFTGSWAYYYEPMYASKLGKVLSVVTSNNQKLNPPHVVLYKKFCFIPVALLFALCLVTFQFLCFFIEIFITQLYSGPLSSILALLPTVLISAFIPVITMIYNKFFVDPMVAWENGPNPKKSKSEKNYVLTFLTSYVPLFITLFFYLPLGHKFTPDMKQAITLYASKFHVPVGASQFIVDINRYKKQFFFYTVTSQIINMVVENILPLVLDNLLPAVLKDGDRPAGEVTSKLDSMVHSRYPQDYRLWKKVESFYASNYGEFDEDRNYSKLVVQFGYIAMFSTIWPLAPLIFTIFDLIILRTDLWRAFIKCKPSSDPTDLQVSKGGAHKVHVSSAPWDNILEKITYLGSVVSVALLLMYRYSGFPGVGLTTGLEKRDSWYRESPLVYSWSNILLVATAVEHLVLLGYIYFKDILISYQTKFEPVTLPSVYIQKPIYTEEVDETAAVMEQVAHKFPVEKNEQTPNEKYSSGMSNTVRSESVSSVSDVKALPSNESNNAVYNGHGSKDESYARASGYNDGFRDGIVDSQKDNDKLYSKDISTSGTNLGINDDEGVASNGFLKPSASTRNRSNSSNGVSRNRSTKRSNTYGTTDAKAHPGEKSSKESRHHHNSNSSTHGEGKGTTPPHIVADSQSREIPVQSQGKSGLNSLPSGDAKSPSKSSKSLGGISGLGETSYSSNRPNSSYLEDNVPDDDAGATLPETIPTSKNYHTRYDKNGKPIKLAAPDPPTSASVESDSRVLSENGPAGEAIAGAESISAGGLSSDLKSGEPSKSARLESALQQEVEDNMSAKSPLSSKMSTAKDSTLKGTKSVKDTANAKSLEHTMKTLDPAAVANSVNNVSTDPSTLSAEAIKVKEKEAPIASTVFNGNSKGAPGDVGSRVPENATGSAEERIATPTSESIAGVSKGSHGHHSKDSSHGVHEEITGVGKASNDNSHDKHTSHGKHTSHGATEDATNVGKSSHSKHISRGLSEDGSDAGKTSHVKKTTKQTVNDNSGNPETVLVSEKTQDTHGKHAGGARHTHETEKSKSTKTADPESPAAQQFGRDVPSITSAGTFGTAESKIDNLTGAIEQSEVTRGATGVKSGSGSIGTPGKANTPSKTATPGTTKSADAPDATVIETASSSARKTPVRSATQSNAGTPRSKGAHSKSGTPSRSKTIGHSSQSKGGSSNAKTADSHKSSAHKGPKSRSSNPELRAKPANGSADSKSKHKSGFLHRMIKKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WVE5</accession>
    <name>A0A8J2WVE5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kelch repeat-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_15500g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WVE5"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="493"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="578"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="779"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="900"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="992"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1064"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1199"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="501"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="515"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="578"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="605"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="691"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="727"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="786"/>
            <end position="795"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF87833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1233" mass="136051" checksum="6E599FB24EDA35CF" modified="2022-05-25" version="1">MAPFKFAKKLTKDKKQSSSSVAGTPPRTSQSGHGPQAGPQGGTQGVSQSVQNGTQGKSNTAPSPEGPFGQNQFAHQNQFHVQNQTNNNKIPSPTPQQPQQVANSQFRVFPTPSPAQQRNASGASTSLGQQKEFTPWNRIKLQNSPFPRYRHVASANCSEDNRLYVIGGLHDQSVYGDIWVISSNESGTQFQSKTVDISETTPPPRVGHAATLCGNAFVLFGGDTHKVNSEGLMDDDLYLFNINSYKWTIPHPIGPRPLGRYGHKISIVAANQMKTKLYLFGGQFDDTYFNDLAVFDLSSFRRPDSHWEFLKPTTFVPPPLTNHTMISYEDQLWVFGGDTLQGLINQVFRYDVASNDWSILETTGSKPPPMQEHAAVLYKHLMVVVGGKDEHDAYLNSVYFLNLKTQEWFKLPVYKAGIPQGRSGHSLTLMKNDKLLIMGGDKFDYARPGEYDLNTSNTDMGKGTLLYTLDLSHLEDMCPGVLKVTDKNGVSYVPATPPVNPMQQQGKLAQPQQPNILTPYSQEGQHTPSGSSTPLQPKPLTPAAYGGVHATPENKDKNNQVTQNQSHINQSVQSIQTGFPKQTGQGTPPIQRAQAGQSGQTAQIGQSGPSGPSVSSSSLGPSGPSGPPGSSSSLGQLGHSGHPGQPGQQAPSSQQDQQNKPDQPAHAGYNEGDQLQYPSPNQYAPQRHSPHYTPQHQPQGQPQHPYHQRGHSRQMSDVKKPVSPIPELERSLSQEEDAPRVASLNSTETKDRPDSITGEVGVATLASSPLKGHVKLGSKSEVSEMGLSQSQTNGYGDSVDSGENGLSEPVQITKHEDVSNSTLQEQRSVSQSRLVGAAPAVGVPSVSSTHSMVASTSDLPTAESRPSISRLSSKEMVKKDAIHHLRSQLEDLRIITEDKALQASEHIKELEVEVERLRSEPSRDVKTADFIKLQSHCDVMEANHSVMQERIQELESMLTAHFLDVGVLNKIIKDQAAKLDGLEQEVTFRDQLHELQAKYENVMAENENLKSQLASSEAEFQKNIKDYSSRLAQFVTQWQERNNPSAIDDLDENGQPKRELVPGSAYHQAAIKKLQQRLDDLLQNNEELTQSREQLTGDHRDLTAKHTALNENLEARERELEARQKELQEAHNNYQEVLNSATNASKALEMSQIELDKYRQVNKKLQNELDELKYVQESNTGNDSFTNGTAGSNHYSMKIKDLKAELYIFKQERDNLKDEVLQLKKKLLNMGEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WVR4</accession>
    <name>A0A8J2WVR4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_19130g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_19130g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="72892at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059018">
        <property type="entry name" value="HEAT_URB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039844">
        <property type="entry name" value="URB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032436">
        <property type="entry name" value="URB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021714">
        <property type="entry name" value="URB1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13500:SF0">
        <property type="entry name" value="NUCLEOLAR PRE-RIBOSOMAL-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13500">
        <property type="entry name" value="NUCLEOLAR PRERIBOSOMAL-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26140">
        <property type="entry name" value="HEAT_URB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16201">
        <property type="entry name" value="NopRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11707">
        <property type="entry name" value="Npa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="URB1 N-terminal" evidence="1">
        <location>
            <begin position="64"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="URB1 central HEAT repeat" evidence="3">
        <location>
            <begin position="570"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="domain" description="URB1 C-terminal" evidence="2">
        <location>
            <begin position="1409"/>
            <end position="1604"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF87991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1681" mass="193543" checksum="873A74A8235A0DEB" modified="2022-05-25" version="1">MDANNTPLAEHAKATENTKFKQSGNIDDEILVKFSHLVTALDAEAESKDFTPLIEFIQKEYAVQAMQTWSYYAQVNNHPKLIEATIVLTKALEVLGSAKNVIEYGGVLIKVILTNYTRVLYRGLNNIRASITIPTIRLMKQMVKYNSGQYIEDFTTNFDFSLHSLPKILTVTKGDIASFNGKNKHHRLYIRFEFIEFWLTLISMCPPLLRKDIISQNFKIMSAWFKQMDKADTVELMDHTLDVLDKKILDEKAFKRMTKTHILNELALSKIYSFLYSSSETLVQKVCQFFLTYGANSETSVAFPCNHLWQDSPISGSSNGVLITVNQKEFRIYNKLLFNILKIFKPWESDIQSSIVLKILNHVPELVPPYCHFLASHGSLDPKMTAYWFGCMTVLGRIIKLKIPQCLETTQTTVIPSLELVIQTALPEPISKLSLTKSLQHEKMIVRYLACQVLIFSFQKLQKILELYDRKGWSFASAPLRNLFYQSVPDLPIITSAMNQSYLIAKNNRVMQLSLTLLLKYYSTTFPSFFQISIPSSNIFVDIMQKDEFSGLDLVIVDNFLQFQELNGIQTKWWNASAREYSLFTSLLKMASSKNSMDAISEKIHQLLKGMLHGTVVFNDLLSSPQRALIHSLQILSFEDSNSEDLSKVWKLLDETISRCIRTPYRYVDMSKNYQKISPFLVVLSEQWKFVDKTSSDIPAKWLCIFLRTMVISGESATGIKKLAEDLLTDVPAECIETYLSFEKYDFKAQKLFSDESYLLSNFQETSFCEHITTMPYEKISKLTRYPLNELDAVGIIWRLQNLMIDSKINPNNRFREIVDILLAKLANYLMSNPTFNIFNSNIIISFFPTDTTVEQEKCYFVAHAIIQLYQELKLTNPSFEQFAFNWLRNHMELLKSTDNNAMNFVFAICSVLNAKDCMELLQSDEEIQAGVLEFLLWKMLRQNHVAIPYKILKSLKNGFASLDELLAQFIEQDRIENFEPDHFIRFSLQNFKQALGAFLHSKWFSLDLLQPYLSQIAGTEVALTVALAIYESNSLAVKEFLQEELGHCIQGVSQQKLSLQGLELFCLCSEMLSEKQKIEILEYATSIYPGKYHSLVVKFVTIANNFELPCVVAWLNKSILYGTKYLSHETQVSGDISEFFSRLEELMNVINIWSKVNHTYLRAQIEVILKGPWVSNSDILQYTMHLLLKGKGDDVQCRKMLQCLLNNENNTLKISNSDAFHRFATAATIFILFKMDSQQNSNRVIQSILLGLYSATITCEDRIILSILEEIETNTSVSWTDELCSLDFLEGEDTGLVEMQSVRKTIVRERQELFVTLDKNIILNTANNYVLRKPVVPSRMTWEDLMSFYRENKKIFASIDGIVYDPTFLMLLSIQSEANLINYSVLGDGSTKCTFEVQRLLSSGMFRILLTSLADIETQAISLHLLHGILHSLEDNKQFRYGEILDILLKKIVYTFESAKQENRLVNISSCVWFAISRIPDLLLNPGTALHEKAYRWVLSSPIILEIDYPLLQDLTTPHERNKDHENFYKQLTWVLECLELGIKTEQDVSFLRNKNVFEWLFNLRNLSYLNGRLCAIIDSIFYKIQRLDSGASVLLTRFAAVSSLEIQKLSLDRKINNATNVLTENKNDKKTLRNKLLLQTQQLNVDELLQSQVETFQSQKRLRDWVEEDQANVIKRICM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WXF9</accession>
    <name>A0A8J2WXF9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_14554g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_14554g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WXF9"/>
    <dbReference type="OrthoDB" id="4060403at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="19"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF88622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="259" mass="29665" checksum="81BD2AA7F2632E91" modified="2022-05-25" version="1">MSRNNDDYLYERFVSESNARSAREQAHETQQSEEELQQELRTAIDNIINTIEQEMQREAHPLQRNEREGAAVNAHEPLFVQRRPFLRLLITNLLLFDYFVVLLLFPFSLYNILRSGFSVITLSENDFLTEIVAYCRYVKVFGDDGKSLLVYGEGKLGLLGKFHNIVVFYSSPIAQCIARFLPSPDAVLRQSAMKAYRFMVKFCTVAMYMTYGIGGTVYLAMAGSFFAICFAITLARRYKSIHRMVAGSLETAVRVPGMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WY13</accession>
    <name>A0A8J2WY13_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Beige protein homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_03488g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">May be involved in protein sorting and cell wall formation.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="26681at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01201">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1540.10:FF:000001">
        <property type="entry name" value="neurobeachin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF123">
        <property type="entry name" value="PROTEIN FAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14844">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="8">
        <location>
            <begin position="1349"/>
            <end position="1479"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="7">
        <location>
            <begin position="1525"/>
            <end position="1819"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="1952"/>
            <end position="1985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1286"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1299"/>
            <end position="1308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00054699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00073334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2145" mass="246224" checksum="67F0D1AE90270893" modified="2022-05-25" version="1">MAMSDEIRTLSNLLGAVLQLTVSDQELDSSVEDHLAEIITNYKCPSDGDEDNELDRKGCELHSYVDSELSIRFQLSNTDNSFEIYAQEEWVRLLENRWDCIEYAVSLQIIVSLAGVSLLNKMNLAKINNLKSSLLERLESETNGDMNPFVKGRLTELCYQVLAVDCKPTDLLRLYKQFDDDFTSYILNSLAAQISDPLSQNYLQFENTYKVCRPSTRELRGTLQFFVEFNNVISNRLATIGKNVYLEIKEGQFCISNDEFIIGLFEDFEFEPALFYSLTLTIDNNVLSLYVDGRRISELSLLEDSITSTNHIELGSMICSFKLFRFVIYEDVLSAESIKIIYALRNTFRESFDSNFDVPGLMVKLGDAFLERLTSTPSPSRVSLKDIFKQIGQLKREHILIILDPSDESGLHIFDKSSSSVHIKLQGIEDSSDDLGVGKCLYYKGANLISMFSSVNCFRFIFCAVQDCQNFDMLYSFLSHLMTLLRNDCLRNWFEKDFGFPMLSHILVTKVIRKVGRSLPIQFLNLFQEYCGWDFSNISNSFIGDEVAYRNLILNFDLWYCYSSTDGESFAGVEIIRFLFFQISSLLGSSHYQKFNSQKLISLDLLENICYLQHFFSEKYGSPNVFYELKTDLTNVYLSLLKENVTKANVQWLLQFSYFELKSGIFENAEVAMNAVDLLFTEFLKIGDSGSVQVFTDSVSPKFLLMVLDEVLNNKGNPAHCLNILFKLLFRKPLVFKNFIKSNGMDLLFDILKNTETSYYESLICLLYARSLGNYDMVPGPGNFAEFEDDQSVFELEVLMKEPFYLAIDLLEWAVINDIRMSNPSELNDLIAIFLSRLSSLLKNPQNCNIFDPRVSPLIMKLNSFFITLTKPQNTSIYESSSEMIKDVICGGMLRAIKAMNFFDFEKYLEALTNSCKYDSNATAIELGLDSNFFEASFLNNFFVEIYNKLNDSEFTLESELTKNPLMLSNLVMFFDKFKRKILTIKFSTSEYLVVLKSVLSCVNFTFKNGINISKNVTKAAEMDMLTHVVIALLYPAFQVDSTWGSKSLEELCKILENYQLCLFDCDNGFCEKGLIKFILSFLLAQLEYQPHLTHVVCCLKAILTRRWKDLPSFAESLGVESSADLKTAFIEICLNEQNIPQAIQNSILFSDAQKGQAIEYALKRMKLTTGINIIDQKGLGERITKYHILQLDTAYHQKRKKHRLFTDDNVTLDKRITSIYRKFYTNFVTDMEQDTVVHDNELSFMAFQHSHTLFLQENLTDNCTWELDSVEDFNRMRRRLLPAPRDFDNELPSTSKGSHIDTKEPGTRSRQWRSNSSLSYDIISDLESIDLSMTDRKDENRKVLKVLKGKDSIKNVWNCSLVIGLDVREGILIIGNMNVYFISNYYFIKNENKVIKLSEVSDAKRDINIGLITGSENRTSQQSSHEVHMWQLSDLIFVTKRPFLLRDVAIELLFENGTNCLISFDGKQYRDVVYSYFEKLPKNRNIDPVLLSILRELNTRSGNIGTKNGITKASLKSKVFKALSSGPSLIDGFAATTQWQKGEISNFYYLIILNTLAGRTFNDLTQYPIFPWVLADYKSNELDLSDPLVYRDLSKPMGAQSKRRETQFIERYQALESLDDENSLPFHYGTHYSSAMIVSSYMIRLKPFADSFLLLQDGKFGHADRLFNAVGRAWSSAAIENTTDVRELIPEFFFLPEFFQNLNSYMFGKGQNGKEVNDVILPPWAKGDPKTFVWRNREALESTYVSEHLNEWIDLIFGFKQRGENAVTSVNVFNNLSYPGAVNLDSINDETERRAVTGIIHNFGQTPLQIFQEPHPPKQFTGMLHINRITWSKMKTSPSRTYQAIFGKRADSSVRYIYRQTDSDGSLYWNGYPFLDVMIKMNDIFLPLKLTSNCSIQIGSEIYEFIHHCQITAFTFWKQNKLITGDKSGLIQLWGYCHSKATNKLSNLGKLYGHLSSIKDMRLYPDYNTLVSVDVSGVVYVWDLIKFRLIRRLSSSGSNIAISQNHGSIAISSTEQKLYVYNLNGLFYTSLTVDCNRKITRLEFLNFSGMDLGRKRHAYWKEKEVIIVGYLDGSLEVYELVLDEKAYWCLKLLKRLETCKGCQITCIKTQLRIHPLDNGEQNPHDIPKIEIMAGDSRGNLFVWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WZ55</accession>
    <name>A0A8J2WZ55_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ZYBA0S04-00166g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_00166g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WZ55"/>
    <dbReference type="OrthoDB" id="20689at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08368">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09391">
        <property type="entry name" value="LIM1_Lrg1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000134">
        <property type="entry name" value="Lrg1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14963">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN 18,19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14963:SF1">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN CONUNDRUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="69"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="468"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="7">
        <location>
            <begin position="781"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF89264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1067" mass="122273" checksum="B9801084971B6997" modified="2022-05-25" version="1">MQAMPDVHPKQIKQQHNNNNSNSKTVVADKTSRNGSKSSHHGSKSSHHGSKSSHNGSKSSHHGSKINRKVCARCNNPITQGSRHGKTTLKALGKYYHEECFTCADCQKFLKPKYFPYDVEETKETILLCQYDYFRRHNLLCHVCNKPLRGLYYTAFGERYDEEHFSCTICGTPCGVKKCFMFQDQLYCKYHFLKYFSKRCSGCHYPISDQYIEFPKGEEIYTWHPECYGIHKYWHVNLSAETLGLPAFPTVKFEKEHRTNSDVNPLPTELEKQMEAFNSILSKTWSVLYKFEEETASCISDMFQYLANSNQLKGISSTALFVLKVECLFKALGSVEDSNFNISPTSGRRKLSLTAPETNHTNENMNLKYSKFPRNLSTKIMIYLQLLRKMSSEPRDAKVTVSSFMSVITGFTHFLKLLTRYGLYNALEKNRQMHSPNSLVRFLREVEKNEKYESDPFSFIDVSINATDNCASCGKYIQEDCIQYHEQRWHSHCFYCCLCQERIKPQDMGDSTYNRESDHILCSHCSVEDPMSLPGFKYVSRLAQLIFLLKIALVRSKAVLEVQLKTRTRNKDGNEVNQSMVMQQTYIRTLNDIKRLKSRREHVQLARDKQEARKSVILETAEADINLKTSEKSKGLVIKTEPASSFDQNSGNVFSNTKTLTLDDISRIVAAEQARELRPNAFTYFKKLKEGDDESVGVVAKKSGVYYSELSSVELNLLRVISLSLLVLDKRIVNEDFDPSQLGPFVLKAEKPSPSSSFWWRMKSKMNKEPRKPDQLKVFGTPLDILSEKYGVESDLGVGPSKIRIPIIVDELISSLRQMDMSVEGIFRKNGNIRRLKELASSIDSNPGGTPDLSKENAIQLSALLKKFIRELPDPLLTTPLYNLWINAAKLEKEHERQRVIYLVYSVLPENNRNVLEVLLSFLCWTGSFSHIENEVGSKMDIHNLSTVITPNILGFPDGNVPKGPLTNTSDSFNDTFAQNEGGHYFLAIEVVDFLITHNEDMGMVPKFLMSLMREVQDKRLEEYETIQQYMHEKVRAKEIDFSEFEVDRTVKMKHSTSLVTKGDANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X067</accession>
    <name>A0A8J2X067_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ZYBA0S04-10836g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_10836g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X067"/>
    <dbReference type="OrthoDB" id="422427at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051711">
        <property type="entry name" value="Stress_Response_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47540:SF1">
        <property type="entry name" value="ACTIVATOR OF STRESS GENES 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47540">
        <property type="entry name" value="THIAMINE REPRESSIBLE GENES REGULATORY PROTEIN THI5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="27"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF89711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="558" mass="64266" checksum="68BC2D476F196D4C" modified="2022-05-25" version="1">MNPKVLVLSMDSSTKKLRPKRLRVPHACDNCRSKKKKCDGKRPCKLCKNAEHECVYSDRRRNVMTPEESEELAPLPPRDVAMKLLWESWNRACVLFRFYHRPSIVKILDSFYEPSQGLTEEQAKARPLIYAILAVGALFAKDDLQEGDVTMREFYQDEGERYFVAAKSMLDVTNIDDVYSIQTMFMLTMFLQCSANLKSCYSYMGIALRAAISEGLHRKNSLVGPTPIEDESKKRLFWTVYKVDIYMNCILGLPQSISQKAVNQELPRDLDDENITSEGCLEQSWGKISSTGLNNEHTKLMLVLSKIHDALYPVLQWNETTYANMLSLQSDLDQWLLQLPMQLKPDYNWVDEAEREEYLKPSKLLHMDYLLARILISKPFIHCIAIEAEEAPHYAFQISVAWNCIQVAHEVVHLAQDMIDGNLLISGYWFSVHTIFYSVTCLMFYQHLLDLSLAKSNGMDVQNDCELGVHILMCLKNGSQAGERSFNVLNSIFKEFNRKTTDLSLQVRATVAPQEPMVTPEELKFFNMESDEYLDRLLKDFLPGEMDLESNFLEELET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X592</accession>
    <name>A0A8J2X592_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BN860_09604g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_09604g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X592"/>
    <dbReference type="OrthoDB" id="20524at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033316">
        <property type="term" value="P:meiotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098813">
        <property type="term" value="P:nuclear chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14131">
        <property type="entry name" value="PKc_Mps1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000377">
        <property type="entry name" value="Checkpoint protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000131">
        <property type="entry name" value="Dual specificity protein kinase TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027084">
        <property type="entry name" value="Mps1_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974:SF21">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974">
        <property type="entry name" value="MIXED LINEAGE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="444"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="164"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="250"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="365"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="169"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF87576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="768" mass="87243" checksum="A190CE55D2DE4EC4" modified="2022-05-25" version="1">MSKRLSIYPAENEKRPLYSQLPSTDDSDDEKISVPPKLSYFGSALVSDKEDDPLGILSRKKSRTSSLATETYANNHTAGHTYVSTHSGEPSQSTLFAQYKNNQQPSMTTMNSTQMEEKHSNDFDSSFRKIKSMQQSMREELTSRHAERRSRRFLSSARIGVLGPAKRASSYNEGSSAASVLSDDSNREELPEARTPLSRIEFGDLNPVQYLKKHNLPSSDLPRISRLYFEKQKNENWQSSRLKHIFPKENVSTGSSKHSPLFSKKENSPATLQPRRKLSDTSLVSGSKNTFYKALSSPVVPSQKNVFLDKTNSFSSRNSEYANTNHSNTFIQSHKKREALANIDINRNNDQSYLQEAKRFKSLKSCDPQDATEKTYKRQASLATTHQVESPPPVPPQNEEFYHREPPKPPVVKRVEIVEPKTRMDSGKGLQAKRNITVVNDEEYEKIELLGRGGSSKVYKVKDNFNKIYALKRVVFDEFDDSSVSGFKGEIELLNKLKDQARVVKLLDYEMEPGVLNLIMECGDHDLSQILNQRAGMPLDIEFVRYHAREMLRCVKVVHDAGIVHSDLKPANFVFVKGILKIIDFGIANAVPDHTVNIYRETQIGTPNYMAPEALVAMNYTQDDGRYQQQNRWKVGKPSDVWSCGCIIYQMIYGRPPYGGFQGQNRLLAIMNPEVKVIFSEKTANDDTVPRSVLDTMKACLLRNPQKRWTVDEILESPFLKPVMVTPFFIRDLIKNAVRYGADQKEVSKDKVEELADDVLNRLADIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5F2</accession>
    <name>A0A8J2X5F2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_14664g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_14664g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5F2"/>
    <dbReference type="OrthoDB" id="272077at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051726">
        <property type="entry name" value="Chitin_Synth_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430:SF3">
        <property type="entry name" value="ACTIVATOR OF C KINASE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430">
        <property type="entry name" value="PROTEIN SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="45"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="212"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="212"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="677" mass="75093" checksum="FBB94E1E40187473" modified="2022-05-25" version="1">MYKEKYRQGNPYGQQFEAFPQTVAREVVAPYPIEDADEVINPVPVFMSSTSNPQSDSTRSQNDNIVRRQKDPYETPFQYGLGPNLNSSDSLAEIRIPPTASMATGTHYSRDDHVPSLDPMPSIPVSVTAVPPPPPYEESETRILQEKAYRVDSPSQQQRTNVNGSESLEHNVNRFQGAQGDGIFNSAGNPRNFGKPDIIGIGGKVGMAATAGTTGKAGNIGNKAGKGRTGSIKSAESTRSAGSAGSAGSVGNTRHIENTGTGTTGASGRKTETVSGDGGAGEKERDLSEYSPRAIEFYKVYKATVADSTNFTHNVQIKWCETLLEYAFDEEFLCHYNINAEKLKRKLRPDESLKNQKVILGHSFRVLTKLVSQKCGPAMYLMGTLYSHQPYLQIKNKNIVDRNDKKALEYYCRAAKTNHSDACYRAGVCFEYQRGTPPEEFSKEHAMQKAFQYYERGAECCQNTSCMYKLGMFYLYGLSGQQHPLQALHWFKMASEKGDSPQALYELGKIYEFTSLPPQVQGVLTGNDIHRDPATALKYFHKCAMDSDYPLAQWKLGYCYEFGELQLPVIAKKSIAWYAKAALAQPKGNPMAMLALSGWYLTGAPDVLKPNDREAFKWAMKACEASDGKLSRAEYALACYYENGIGCRRDLHQAKAHFETATKLGHVKAKDRLERDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5J3</accession>
    <name>A0A8J2X5J3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">U3 small nucleolar ribonucleoprotein protein IMP3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">U3 small nucleolar ribonucleoprotein protein imp3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_15038g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5J3"/>
    <dbReference type="OrthoDB" id="10248812at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034457">
        <property type="term" value="C:Mpp10 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000006">
        <property type="entry name" value="U3 small nucleolar ribonucleoprotein IMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="12">
        <location>
            <begin position="4"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="RNA-binding S4" evidence="11">
        <location>
            <begin position="109"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF87812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="183" mass="21855" checksum="7F8596F56596F7F2" modified="2022-05-25" version="1">MVRKLKHHEQKLLKKVDFFDWKQDQGHRDTQVMRTYHIQNREDYHKYNRICGDIRRLAHRLSLLPPEDPFRRKQEVLLLEKLYAMGILATKSKISDLENKVTVSALCRRRLPVIMHRLRMAETVSDAVKFIEQGHVRVGPNLITDPAYLVTRNMEDYVTWTDNSKIKKNVLRYRNQIDDFDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5J6</accession>
    <name>A0A8J2X5J6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">BN860_17172g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_17172g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5J6"/>
    <dbReference type="OrthoDB" id="42889at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF147">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EFM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF87907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="574" mass="65996" checksum="B559E358B6634D48" modified="2022-05-25" version="1">MKPTISALTDWAKDNGAEISPEIEFTEDAVKGICAVSSAELSNAKIKVPESIIISSQLASTYFPVNPGDGNTWIKFLLCRLKFQEELRFGPYIRNLPQTLNSPLVWNPREWQLLEGTNLGNSLKDKLYKIFTEWYEFVLKYSHIFASSPLSHDIDLYKRFDAMGPDELYVDVLQHAAKGDTQLWYSFSAFLWAHLILLSRAFPEYIIHPGCNEGSVILLPLLDLLNHDAYTKVQWYSTNNNFYYEKLETTGAGQELTNNYGPKGNEELLMGYGFVEECNEFDYVALKIRVPFETLEAILHSEDSIQLPSLDDYTTFAFERPQTQPAKEKCNALFYKDGVTYFINARDDKCLIPLLELFSYLSKAQTEKWRDLRPRLQGLQALRTALQSKLQQLSLFSDISDSKTYSIDKYRRHCADIYRRGQHSVLKHSISSIKAVEKSLIKQCGDRLLNLKKIIKNDSRFTQDELPSLFKGEDQNRVSFEDTLEIIALWVASKVKYDTFNSKHMWIGQQFHSFLKRSLSLEVTQDVKALYAELFPQNKAEISLDEVNLAYNFVLENSFTRSSSSNETILVRNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5K6</accession>
    <name>A0A8J2X5K6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_15720g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_15720g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5K6"/>
    <dbReference type="OrthoDB" id="424302at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF10">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="7">
        <location>
            <begin position="9"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="186"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="341" mass="38809" checksum="CF0F906FCF8FBBB1" modified="2022-05-25" version="1">MSNESSDEETVEDPQLRSFHSYLRQARKILCVVGSGLSQSSGIPTYQLQRGTWKGYTSLDLATPEAFQSNPGLVWLFYSNRRYIAMKAKPNDGHFALARLAKNMKERHREVFVVTQNVDGLHQRTGQAEEDLCELHGSLFQLRCTSFFCTYKGKNTKDLFLTSALREFTPRDVGVKRKLRHQLPSLTKRRKKQDITKKEEDGEESLSSSDFEPLPELKEDSLPHCPKCGEGLLRPGVVWFGEPLPLMEMVKVDKFFAPEEENANDHIDLVLVIGTGGKVWPAMGYVERCKQNGGRVAIFNTHIEDKEKVRKDKTLWGFEGEAAVWLPRALEPLIGKHFRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5L4</accession>
    <name>A0A8J2X5L4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">BN860_18228g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_18228g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5L4"/>
    <dbReference type="OrthoDB" id="4031914at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF87953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="204" mass="24186" checksum="118523894B68D4DF" modified="2022-05-25" version="1">MDGSSSDNFEYILQLTKILSAECRANRQERDKVEHLFKRLAKQSYVGYEHLSGNVTPKSRELFNKIATPTEEDKLIKENYDLLKQIELQEYMNNKFWLLINEINEHLSSIRNFMIERKLSGSQDTANFIDEEFTMNCQRLDMSTDVLKRELNVTKEKSALVEQELKQLLEQIDWSEVPKNSREYINLHEQIQVLKNRYAVRFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5U1</accession>
    <name>A0A8J2X5U1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_19372g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_19372g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF88001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5U1"/>
    <dbReference type="OrthoDB" id="2943660at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF29">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="11"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF88001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="822" mass="95026" checksum="EDE8E693BAC27894" modified="2022-05-25" version="1">MSVRRPRKRLVCVQCKATKRRCDKLRPICSRCRRSSLKCSYEENPQSSRVMTDVSPVKTFPINKADTQLTYSFLSGRRMDLQDVRIDPLKIWNPEERLIVIGCTTFLDYPFSAHSLIQCDHYSRALSGSLHGMTLLDLSNHFNRIPVDGTTPRFPGPLSFIEKAIIKSVKHSEMNRFQPPIPGVFYDPCAVDDDSTRDALKIVLAEIEDASMPRKDCAMLLKRFYQKIYPCYPFIDIEHFENDIMKLYGESDEDHWKIKPNSKDVRKQMETLSLLTIFSSISLKNSTLDENQLSSTKANATEAASRLSNLCHKLLCLLDVFRYPSENTFACLLYFYINGFLDPEDPDVMPTPARLLTLRHLTSLAITLGLQYEPSNYKRITDPHVINLRRKLWLGIQSLRFQFILTEGDSDITNQKYMENFLLVTKNSGDLFNNGACFTVEHNSRNLNISRHKYQFHRLLFKLVSSCAPIVGEVQLNRVLENVKRSEEYIFEHFPISNIIENCEEPAYKTLTFSGDTKFDIEGVGKAEVFSMNIIGYTCFLNVWNVLGLYFEKECLVDWEKYEETYHFFMAKSFAVYLELAGMIFDYLDNKFQNSIPREFEYILDKPVCFALLRIWMYQCHILLRLSYKKEMQQKHLESKTIAQDKSEKEKTNALISKLLVCVRNQMSILLELANVKLSGKYSCAFKTIPMFRYILYVTDLGQLTSVTNEFWKRATEEKKVPQYIEQNIFLKWGLNAKSGNSILQFLTNGQTLGSFNGTLLSEIEKLVSSSSFGNISENATSEDHLEEFLNGNEENILSQLLQNNVDIFWELLGEDFNNSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X651</accession>
    <name>A0A8J2X651_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BN860_07470g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_07470g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC22 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X651"/>
    <dbReference type="OrthoDB" id="361797at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050781">
        <property type="entry name" value="CWC22_splicing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034:SF4">
        <property type="entry name" value="NUCLEOLAR MIF4G DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MI" evidence="5">
        <location>
            <begin position="644"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="16"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="130"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="209"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="253"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="278"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF88326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="893" mass="102401" checksum="9C46E419B1455C8B" modified="2022-05-25" version="1">MASKHGIRIPGILLDELKARGDANDDRYYSGKGKKRNTKSQLSRKETRKRQRQEKRNNMRSNTIQSTPKSQKKADKPVTLKTNTLPFSSDDELSPDDFDEFIEDSSDDKHEHEQCMTAEETMAALKAVKDAKTPRLEDTKTKKTSQSKEKEIEGVRPLTPAEREAMKRDEIEMEYYAKKLGLKGKNKKIHARDEFDAIGGLLDGLEFFENFEGSKDQEEEEADMESDQENSFEEEQIEEADFSEDNIQNPFSSDDELSSGDFDDNDELLDEDESEGGEDSRREYEDSSKESAKENPFVAPIAEDDNTYVPPSLRKHKLAIEGELETLTRLRKTVKSSLNKLSDSNITTIVSSLNDLFDSNSRQYVTDAITKEVLAIICQSNKLLDGFIMNYAAVVFSLWKLRGTEVGALFIQSFVEEFLKYYYQQMDVVQNFNSSKEAAAIFPKECSNLITLLAYCYNFGFISCKLIYDLIRYFVKSPNEFTTELLLKVVSISGPLIRGDDPSALKEIISNLLANVKSIKDQPVRLRFLLETMSDLKNNRLKPSLLATSHQPVKKVLQNVIKSSNSSSEPLQVTVDDIRNIDKKGKWWLVGASWKGNLESAFEEADNEGTKPTESIQKINLKDDLLEDMPDWSVIARQQRMNTDVRRAIFVSIMSAQDYMDAFTRLEKLNLKNKQVAEIPRVLIHCLVQDSNTNGYNPYYALLARRLCEYQHHLAKSFQFLFWDIVKRFEDEDPDDEEDKEMNEDARLRSVANRGRFFGFLISAAILPLDVFKHVPIISGLNSEGNLFVEVLLYQLLLNVAKNSEIKTRDSGGKKIIRFKNDELTKLLVNGVKLENRSVILKGLKWFITRKFKYNDYVGQKGDKKYEKDLRRIEWAVPTIMDIINHELEGITY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6C0</accession>
    <name>A0A8J2X6C0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_10484g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6C0"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17982">
        <property type="entry name" value="DEXHc_DHX37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003770">
        <property type="entry name" value="ATP-dependent RNA helicase DHR1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000637">
        <property type="entry name" value="ATP-dependent RNA helicase DHX37/DHR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF99">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="415"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="689"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="169"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="245"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="704"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="185"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="252"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="268"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="302"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="321"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="705"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF88447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1258" mass="143735" checksum="647552B26C92D2A5" modified="2022-05-25" version="1">MGTYRKRFNEKARAGQIAKQNRLKRIRNKQFLRTIENRHDDKEETLSDDHENTPQHDSNASILPPVSKEEEDLKKRKLQELFAPKESKVSRIKRKRLDKFIDHQLRKEEKKALMNKLQDYKIDTSLLTSSKRLGHGKQTKKERFQEALTLEKQGRADANTKDILYEEHEQKNWFEDYPQNTENDRSEDEQVSEEEDIETKSSKFIDFRPVKGSGMGFGFANAKVVNRHKPSKKKYKWRQLVEMEEKKKHDVEDDNDFMSTGDEEDDSVSNVSEDTSVSEESATEISEDKVLEENNSSKNLSYDDDTDESVQENLSEDIEDSSNSPQNTAESFKQWANEQIRELEGRNFNLATPSLDYDYEPIVRQEDLEDGLEADEIPIDEKSKRKAFYVEVSRPERVQQVRMQLPVFGEEHRIMEAIHHNDVIIICGATGSGKTTQVPQFMYESGFGCPQSPDYPGMIGITQPRRVAAIAMAKRVSNELGNHGDKVAHQIRFDSTVQSNTRLKFMTDGVLLREMMQDFRLRDYSSIVIDEAHERNINTDILIGMLSRCVRLRAKEHARDPENNKKLKLVIMSATLRVADFSDNTTLFVSPPPIINVEARQFPVAIHFDRRTAFNYVEEAFRKTCKIHQRLPPGAILVFLTGQQEINHMVKKLRREFPFPRGNKNRKEMESPVSNVKVNSKNADMEAEDIDFSVKILENDNLSYIEEDEDQNNEGEEEAEEGFDETLEDGQNANDPLYVLPLYSLLSTKEQMKVFDQPPPGSRLCIVATNVAETSLTIPGVRYVVDCGRSKERTYDESNGVQSFEIGWISKASADQRSGRAGRTGPGHCYRLYSSAVFEEDFPQFSSPEILRMPVESVVLQMKSMAIQNVTNFPFPTPPDPRNLMTAITVLQHLGALDAKEKITEEGKVMNLFPLSPRYSKILLVSTEQKCLSYAISIVSTLSVGDPFLKEQEIKPSDGDYNSGEEEVNDEQKRLLLKNFHKSKVKFSKLDPHSDVFRLLSVVSAFDYVPRKQRAAFIRENFLRGKFLEEICKLRKQLTYIVKSNTSQENVAVKVSDDDLKSSLPTEIQLKMLKQMICAGFIDQVAIRADYLFPDDVQLTNKMSIVNIPYVSVLSPKSPDITSSFVYIHPSSVLASLGAAPPKFLVYHSLHSSSNSKTRMTSLCDIKSTPLANVARKGSLLTYSKPLTGKGLKTINLTHKERYCYVVPRFGDSTNSDLSISWDLNPVPVHEVKEKGQWVVTKFITNKSFEDQRKRSNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6D2</accession>
    <name>A0A8J2X6D2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_15060g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_15060g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6D2"/>
    <dbReference type="OrthoDB" id="4035953at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014805">
        <property type="entry name" value="SKG6/TOS2-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08693">
        <property type="entry name" value="SKG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="633"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="695"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="218"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="709"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="718" mass="80878" checksum="8A0CB2B93557F2DD" modified="2022-05-25" version="1">MNTDSISVASTIPDAIDPWLLDRTRSLVKRSDDSGDDDDDQVHKNKNNGTVGVTVGVAVAVPVGVCLIVLAVVLWFVYRRNKREAKDDHDPDFTGDTEYLPNPSQIYDDSQSCMSPGDSQEKGDSDSYLPPKIKNRMTGTRVLSVDPFRIPERNDTVTIRDFARQVHDQGYGPYQIASNSNSRNASSYELSHPRSGQHHSYTFNALTSKEKVHVHNASTSRSSSISEGRPPSAYHSPVKSVAGEVTRQFAEDDSGLDVDESGPDTSFARSKEAIVQQPDFLNSRDEDLPLTEREEEDIKRMRSVYKVYWEKNHQNGTEQTTEYPELPTLDPSIDVSAPDTSATVPPAQNHLMVPTAQEQHQRAASSIYSSVPTTIISPEKQAPFQEQPVQHIQQSVQEQVQPAQHIQQPMQEQVQHIQQPVQERAQHIQQPVQEQVQHIQQPMQEQAQYIQQPMQEQAQHIQQPMQEPVQHIHQPMQQQMQQPIYDAQSYGSAQYYPQQYTPQQYTPQQYPPSQFNMSPMQYAPPSSYGYNMAMQQQYQRAHPQTLESIDELPTPTNLPYSSSSLSLTSFRKKPRQAAALPPLQAARVNGTALNPMDHPEMFYSNNQTSGHNNGTAVAPYQMRQSVVMTNPSDLTLPTKFRPAGSIRNVSGMNTRNNSMTTQMNPYYQQQVAYNSRVSGLLQEEDVLHPSNIEGILPHNGSPDDLRRQLGTSHNYNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6F5</accession>
    <name>A0A8J2X6F5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_16094g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_16094g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6F5"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF31">
        <property type="entry name" value="VITAMIN H TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="428"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="480"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="512"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="546"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="611" mass="71046" checksum="DF81799090226EA6" modified="2022-05-25" version="1">MVSGWKKFLPHLRVLPEDLEVSDIQSSSSGHASIVEIQEQDKLQEKDSIKDVKQDDGIVDVENFGTKTEGSGKISNVEVEHPLGLKPDMPYELRDDAGRKWWKYFDEYEYRLNKQYRKSRKWYEFLYPNHATQSKAEKSLLYKLDVILAFYCLILNWSKSLDSNNYTNAYVSGMKEDLKMKGNDYIYTSTIANVGSIVFQLPFMYFLPRFPSHIVLPVTDLGWTWFTFACYRANSLRELRGYRFIESAFGAAYYPMSQYLLGSWYAPDEISSRVALFFCGQMLGGVTSGLLQGRIYESLNGRNGIAGWRWMFLIDSMAISLPTVILGFYLIPGVPSKCYSLFLTDEEIRAARKRNKRVGVNDAKDREQLKPLWDLKIWKTVLFTSTFWVLVVFDTCSWNNMTAFSGSYTLWLSSHKDYSVEKVNNLSVLPPCLGFAYVLFCAFGADLFRCKWGFMVFSQVMNTISCAILIRWDVRNTTKWYAFMTTYFSVAASPSLWSFINDFLRFDPQIKAVTWIAIYSISQSTYAWIPTLAWPTKDSPRFKRGYIVSIVFGTIYMLWTFVVLFFYKKNEKKHALDNGIVLYNTEKGEEPPTWLETDMEKRDGFYYLKEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6U6</accession>
    <name>A0A8J2X6U6_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Adenine deaminase</fullName>
            <shortName evidence="7">ADE</shortName>
            <ecNumber evidence="7">3.5.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Adenine aminohydrolase</fullName>
            <shortName evidence="7">AAH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">AAH1</name>
        <name evidence="9" type="ORF">BN860_14752g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolytic deamination of adenine to hypoxanthine. Plays an important role in the purine salvage pathway and in nitrogen catabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>adenine + H2O + H(+) = hypoxanthine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23688"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family. Adenine deaminase type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.2" evidence="7"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6U6"/>
    <dbReference type="OrthoDB" id="272271at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
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        <property type="entry name" value="ADENINE DEAMINASE"/>
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    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="5 7" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Adenosine deaminase" evidence="8">
        <location>
            <begin position="11"/>
            <end position="339"/>
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        <location>
            <position position="16"/>
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        <ligand>
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        <location>
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        <location>
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        <ligand>
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        <location>
            <position position="228"/>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="348" mass="39957" checksum="16CB52AFE6DEF58D" modified="2022-05-25" version="1">MVSREFLQELPKCEHHLHLEGTLEPDLLFPLAKRNGITLPAEFPQSVAELEAKYCQFRDLQDFLDYYYIGTNVLIEEQDFFDLAWAYFQRVHQQGLVHAEVFYDPQSHTTRSIPLERVTRGFQRACERAEKELGITSKLIMCLLRHMEPEHCAETIEQARPFIEQGIIHGLGLDSAEKPFPPELFVECYEKARSINGDLKLTAHAGEEGPARYVSDSLDLLQASRIDHGINSVHDDELMQRLAREQVMLTVCPLSNVKLQVVQRVAQLPLQKLLDSNVPFSLNSDDPAYFGGYILDNYAKVAEEFPQWDHAVWARICRHSIEGSWCDDKRKTELLQRLQSVVQKHASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X825</accession>
    <name>A0A8J2X825_ZYGB2</name>
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        <submittedName>
            <fullName evidence="3">ZYBA0S04-07712g1_1</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">BN860_07712g</name>
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        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
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        </lineage>
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        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
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        <text evidence="1">Belongs to the RNR ribonuclease family.</text>
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        <property type="entry name" value="NA-bd_OB-fold"/>
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        <property type="entry name" value="RNase_II/R_CS"/>
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    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
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        <property type="entry name" value="EXORIBONUCLEASE II, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS01175">
        <property type="entry name" value="RIBONUCLEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNB" evidence="2">
        <location>
            <begin position="504"/>
            <end position="836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="RuleBase" id="RU003901"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="CDF89581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
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            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="950" mass="107920" checksum="7574645EFF2C8D94" modified="2022-05-25" version="1">MSQIFARRLHVTGLCLRQAKKPVKRGQTLGKQANNVLSSPIRAMVDPPPQLTQTDIDKINANFLSRTKDLEPDLEVKDLAQIRQEFHSRYRARYLNPSKTWFELNWISKEKPIFSKFLINASVEDMGADRNESFDFDVADLMSRPLQVGDLVLIKSHPSELCMCIDIPASTRDPRYTFTTVDGTMIFTTRSMVSIRIPHSLSNVTVDAPLLIQEPKHGFETVGYVKNKASQTFILPVIPRQQITSPISHEISKKAWEALPCILKKMELLHRHLQEGRGPWQVPFFNLVNMAEQLDIQLASASNTGAVYVESLIKSCMSKTATKINSSTLLATFWAIENQQQSHLWGEVHTNRALLSPVSVTVMPLSSQYLYYGRVLQNLKAKNYDAVTRFAQLVDAGKNTVAVEEFPEIIKLLQDYAAGNFHKDATGIFIVSKIFRNIKRFKEEDITRDICHQLLEELDPVQKSLNPLHYNLDLGLPPSSTKANAEEQIYQLAMPVIADDSSERHDFGNMKVYCIDSDMAHEIDDGISIERHENDNYILHVHIADPASFFPESRETTKGINNDILKIALKKSFTTYLPDLVVPMLPQSFCKAADLGKQNQKTKTLSFSVGAKWSRKEGFRLLSNTFQVRLGLVSNFPKVTYETVDSILEQASKSKKGKKLTDVQEDLKSLHAIARELRDYRVKVQHAVLFGDSFNRVQVELSPSQSGVSQQVSFKDQVETPSTVLVSEMMILANTLSGEFFRKNKIPGVFRCYKSLKLGIQAKAEYDLMKMKMQGDKPLSVRDINMMATLLNSSYYSGYPYSHEMIGAPQYLTVTSPLRRFPDMINHLQLHRYLLGLPFCFTKDEVDRLVWQIQSRDAILKAAGRHSNTYWTLKYLKELIERDRNCKFDVMVNSVPQVGFVRCILSDFSSARAILKFKPDEISYPKIGDIVKDCKITKIDALEGLMEMSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8H7</accession>
    <name>A0A8J2X8H7_ZYGB2</name>
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        <submittedName>
            <fullName evidence="2">BN860_14422g1_1</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">BN860_14422g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="4069015at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="905"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1082"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="341"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="366"/>
        </location>
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        <location>
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        </location>
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        </location>
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        <location>
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        </location>
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        <location>
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        </location>
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        </location>
    </feature>
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        <location>
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        <location>
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    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
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        <location>
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        <location>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="962"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1013"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1098"/>
            <end position="1118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1132" mass="121774" checksum="ED6F4B2CC3E0264F" modified="2022-05-25" version="1">MGLLTQRKEHRVPDLSRYDYYYQQRDDYNKSPRLSAAAAYAASRSRGNPVLVQQRSQSFTQPRRIVRSSSTATRRRAKSKSTATSSTTPRANSFATTTRIQDQQGRTKSITRRTVRRINGVDYVETVTTTPLDQEDIAATAHHFDEFSGYQGGSYADAAEYPEETQEHEPHREPHREPVRRIVREKVTRKVKRVPDSPRSERSQRSPRSQRSSMAVSPQAGEFTQYTSEPSPPPVPTHTRVIRRRRPQRSRSQRTAKGVPAPASTPAPRRLTEEEMYAKALEIAQRNVYSNSDFPPEVLEATARAQSEQGASRMGQRSLRKGDARGRKKFGALFHRDREPSGATTTSPLSAAAAAGGTGAGAGGAGSSIAMGSSTGGPASISAPRVSGVSSIDHHRADPSAASGSTVSRRNLSDEEMYAQALAMSQSKRQRSLDESLGEPLPLEESLPRPPPLEEVETEPLDLGGFRSPHSQTAPSFVTPAQTIDEPYEDAVEGQSVVLEAPQEEPEESHQNLGKQEPEEEEEEEPEEPDWRDAYGHEPKTPYDAEASYGSHDREYQQGYQQGYEEAEEIQQGAVQVESEPEIEQFEPGSDLKHELEPESDPEPEQEQEQEQEHQSEPEYDFEEPQYHPRYKRRSRAFSGRSGRSGLASPIDTTGFRSKDSAMPSTAPSLEGRQTVRASFESDASSDSRVAAQTTVAAAATPAATPAASAPARSAMRSTVVKRSGAVPATTATANGSHIARTAGIGSSKAGTASPAATSPAAAVPAADPRTHGGLDNEADLREVERKPRGLSRFRTRRSFFGGRDDSAQEKAEQQQKQKQQYEQKLRQEAEKLAGTSTPAGIASNAGATTNAKTVATSKISATSRTAPSTGFGAGTSAGSAFGTQASILGGGATSASEIPDQEIGYASHPYDNRTDNRNDNRTDIGTGGETRVETRPKGRFKSVFDKIKEFGAENSGYQPPREVREAQRAERLSGFETVETEAKPRKSHPWRGKRETAGGSKVGVNGNGGALGVTNGHAAATATTTTNTTTTTAVPGAGVAASGAGGAGDEPSLSSYYGDKEPLAEEGFADGYADALAANGAGNAQSSGVREKAHQAAESSFGQQAKSKAKNEAQSQAKEQKSNLLKKLFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8H9</accession>
    <name>A0A8J2X8H9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ZYBA0S05-04478g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_04478g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF89906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001262">
        <property type="entry name" value="ABC transporter CDR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005285">
        <property type="entry name" value="Drug-R_PDR/CDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00956">
        <property type="entry name" value="3a01205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="506"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="539"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="590"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="617"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="648"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="756"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1188"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1220"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1259"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1308"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1461"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="143"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="852"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="41"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="801"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1134"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF89906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1498" mass="169512" checksum="1CCD4ED7BB570864" modified="2022-05-25" version="1">MVVGSVEQHPGGYSDENAYGGFDEAINGSVKELARSLIEENKDSTNSFSERENTVENNTGINPVGLQPSDAEYKPELDPSSNDFSSKAWIGNLAKVVVSDPDYYKPYTVGCGWRNLSALGASADVAYQTTVDNMPWKILSWLYRMARPAKESDTFEILKPMDGLVKPGELLVVLGRPGSGCTTLLKSISSNTHGFKIPKDATISYSGLSPKDIINHFRGEVVYCPEADIHLPHLTVFQTLLTVARLKTPRNRIRGVSREAWARHVTEVTMATYGLSHTRNTKVGNELVRGVSGGERKRVSIAEVTICGSKFQCWDNATRGLDAATALEFVRALKTQTEIVHSAGCVAIYQCSQDAYDLFDKVCVLHGGYQIFFGPATKAKSYFERMGYYCPSRQTTADFLTSVTSPAERIINREFTEKGIAVPQTAEEMSDYWRNSPEYQELIAEVDETISQDHEKSLEVIQESHNARQSKRARRAEPYTVSYFMQVKYLMIRNFWRIINSSSITVFQIIGNSVMALLLGSMFYKVLKKSSTGTFYYRGAAMFFAILFNAFSSLLEIFSLYEARPVTEKHKTYSLYRPSADAFASVLSEIPAKVLTAVCFNIAFYFLVNFRRDAGRFFFYFLINIIAVFVMSHMYRCVGSLTKTLTEAMVPASILLLAMAMYTGFAIPKTKMLGWSKWIWWINPLSYLFESLMGNEFHDQKFPCTTFVPRGGDYDHVTGTERVCSVVGSKAGQDYVLGDDYLKESYGYLIKHKWRGFGVGMAYLIFFFFLYLFLCEVNEGAKQKGEILVFPRSIVRKMRKQNKLKEEDRDPEDVEKIAGSSGSTDKMLLKDSSESIDEENEPSALGGSQAIFHWRNLCYEVQIKGDTRRILNNVDGWVKPGTLTALMGASGAGKTTLLDCLAERVTMGVITGDIFVNGKIRDESFPRSIGYCQQQDLHLKTATVRESLIFSAMLRQPKSVPVSEKKKYVDDMIRILEMEQYADAVVGVPGEGLNVEQRKRLTIGVELVAKPKLLVFLDEPTSGLDSQTAWSICQLMKKLSKHGQAILCTIHQPSAMLMQEFDRLLFLQKGGNTVYFGDLGKDCKTMIDYFESNGADPCPPDANPAEWMLEVVGAAPGTHANRDYHEAWRNSPEYQAVQQELDRLENELQSLDEEDGVEKHKSFATDVFTQIKFVSFRLQQQYWRSPQYLWSKFFLTVISELFIGFTFFKADRSMQGLQNQMLAVFMFTVIFNAILEQYLPNYVEQRDLYEARERPSRTFSWFAFIVSQILIEAPWNFLAGTIAYFIYYYPIGFYQNASAAGQLHERGALFFLWSTAFYVWVGSMALLANSFIEHDVSAANLANLCFTLALSFCGVMTTPDAMPHFWIFMYRVSPLTYFIDAVLAVGIANVDIECSNYEFVQFTPPQGRTCGEYMQAYLKSAGTGYLKDANATAQCLLCPLSRTNDYLSQVNSHYSHRWRNYGIFICYIVFNYVAAVFLYWLARVPKKDTFFSKIFSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8L7</accession>
    <name>A0A8J2X8L7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA sliding clamp PCNA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_08504g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair.</text>
    </comment>
    <comment type="function">
        <text evidence="7">This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processivity during elongation of the leading strand.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the PCNA family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8L7"/>
    <dbReference type="OrthoDB" id="534348at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043626">
        <property type="term" value="C:PCNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.150.10:FF:000006">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.150.10:FF:000011">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.150.10">
        <property type="entry name" value="DNA Polymerase III, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022659">
        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00293">
        <property type="entry name" value="PCNA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA N-terminal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA C-terminal" evidence="10">
        <location>
            <begin position="127"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF89614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="258" mass="28905" checksum="133C03D1C6C27D32" modified="2022-05-25" version="1">MLEAKFEEASLFKRIIDGFKDCVQLVNFQCKEDGIIAQAVDDSRVLLVSLEIGIEAFQEYRCDHPVTLGIDLNSLSKVLRCGNNTDTLTLIADDMPDSVLLLFEDTKKDRIAEYSLKLMDIDADFLKIEELAYDTTLLMPSSEFTKIVRDLSQLSDSLNIMITKETIKFVADGDIGSGSVILKPFVNMDKPEDSIKLEMDQPVDLTFGAKYLLDIVKGSALSEKVGIRLSSEAPALFQFDLKSGYLQFFLAPKFNDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8P4</accession>
    <name>A0A8J2X8P4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="7">PPIase</shortName>
            <ecNumber evidence="7">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_17634g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="7"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8P4"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000019">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0697">Rotamase</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035339546" description="Peptidyl-prolyl cis-trans isomerase" evidence="7">
        <location>
            <begin position="21"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="8">
        <location>
            <begin position="30"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF87927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="208" mass="22966" checksum="4176106CDB222197" modified="2022-05-25" version="1">MILSQLLLFVFALFATHVLAEDPPITSKVYFDVKHGDEEIGRIVIGLFGETVPKTAENFYELTISKDSDMGYLNSVFHRVIPKFMIQGGDFTDGTGIGGKSIYGNTFPDENFEIKHDKPGRVSMANRGKDTNGSQFFITTVPTPWLDGRHVVFGQVLKGMEVVTYIENVSTNSMNAPKVPVTITGCGEVETVPPDGYPEHYASDHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8P6</accession>
    <name>A0A8J2X8P6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ZYBA0S04-09978g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_09978g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8P6"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000006">
        <property type="entry name" value="General amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF15">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="150"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="399"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="428"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="471"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="505"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="10">
        <location>
            <begin position="63"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF89676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="578" mass="65201" checksum="1B79AEDDE3ACF2B7" modified="2022-05-25" version="1">MLNAERFHDPEFSPKKNSWVETLQREPSERSEQSEQSEVSDGESQRTLVRSTRHVQRKLSNRHVQLISISGVIGTALFVSIGKALYHGGPASLLIGFAVWCVPIMCITVSSAEMVSFLPISSPFLRLTALCVDDSLAVMASWNFWFLECVQIPFEIVSVNTIIHYWRDDYSAAIPLVVQLVLYALISFCTVRYYGEVEFWMASFKIILAIGLFLFTFVTMVGGNPRHDRYGFRYYKEDPFKKYWPNGHGSDASGHFQGFLACVIQASFTVAGGEYVSMLAGEVILPRKVMPKAFKQVFVRLTVLFLGSCLCVGILCSPNDPDLTAAINESRPGAGSSPYVIAMKHLHVHVLPHIVNAALITAAFSSGNAYTYCSSRTLYGMALDGYAPKIFAHCTPQGTPVYAMIVSLAWGLLSLLQLNDNSAVVLNWLINLITTSQLINFVVLCVTYLFFRRAYQVNRYKLPDLPFKSWWQPYPAIVGIVCAGSMTLIQGYTVFFPSLWDVQQFLFSYLMLFIDIGIYLGYKFLWRRGKDKFKDPKQVDFLPELTEVDAHENHLHFNKYCEANVGDRPVIDNFSEMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X937</accession>
    <name>A0A8J2X937_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA replication licensing factor MCM2</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">DNA replication licensing factor mcm2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Minichromosome maintenance protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">BN860_07800g</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X937"/>
    <dbReference type="OrthoDB" id="844at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17753">
        <property type="entry name" value="MCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000002">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000138">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008045">
        <property type="entry name" value="MCM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF44">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12619">
        <property type="entry name" value="MCM2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01658">
        <property type="entry name" value="MCMPROTEIN2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="21">
        <location>
            <begin position="495"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="724"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00074927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00078186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CDF90049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="865" mass="97908" checksum="2F3B7B0E36D52B8B" modified="2022-05-25" version="1">MTDNRRRRRENEDSDSDNEIPPSSPQPLHRAAYDTISSPIGSPDADDMANPEGGDNEIEDDPDLDELEEQMNEVDLMADDMYGDYEADPTNDRYDDANVDDSQQKELSLAERRRIDAQLNQRDRLLKNEAYMVDDEDIDPAAGQLDEMGLPIQRRRRRRQYDDVDIDEDLLDDMEIDPLTEELTLESLSNVKANSYAEWITQPNVSRTIARELKSFLLEYTDETGRSVYGARIRTLGETNSESLEVNYRHLAESKAILALFLAKCPGEMLKIFDLVAMEATELHYPDYARIHSEIHVRISDFPAVYNLRELREANLNTLVRVTGVVTRRTGVFPQLKYVKFNCLKCGSILGPFFQDSNEEIRISFCTNCRSKGPFTVNGEKTVYRNYQRITLQEAPGTVPAGRLPRHREVILLSDLVDISKPGEEVEVTGIYKNNYDGHLNAKNGFPVFATILEANSIKRREGNALNPEEEGLDVFSWTEEEEREFRKMSRERGIIDKIISSMAPSIYGHRDIKTAIACSLFSGVPKNVNGKHAIRGDINILLLGDPGTAKSQILKYVEKTAHRAVFATGQGASAVGLTASVRKDPITKEWTLEGGALVLADKGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQARCSIIAAANPNGGRYNSTLPLAQNVNLTEPILSRFDILCVVRDFVDEEADGRLAAFVVDSHIRSHPENDPDELERGDDIATEEANEMARLSARERRLQKQRKKEEEVSPLPQELLMKYIHYARTKVYPKLHQMDMNKVGRVYADLRRESISTGSFPITVRHLESILRIAESFAKMRLSEFVSSWDLDRAIKVVVDSFVDAQKISVRRQLQRSFAIYTLGHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9Q7</accession>
    <name>A0A8J2X9Q7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ZYBA0S07-05644g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_05644g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
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        <property type="entry name" value="Mto1-like_Mto2p-bd"/>
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        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="689"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="17"/>
            <end position="53"/>
        </location>
    </feature>
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            <begin position="69"/>
            <end position="106"/>
        </location>
    </feature>
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        <location>
            <begin position="393"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="432"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="710"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12808"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CDF90636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="737" mass="84357" checksum="F2C42FB9D3C207DD" modified="2022-05-25" version="1">MHFTKTSTASLPNVIGNELISGSKSDTEVGDMVSKESKDLSDVDDNYNGQREDITSGLTLPRLRDSITPWRNSEKPAVTMKPRRGRSNTSLEVSSPVNSRPTTRNSLFSADGAYTITSSSASSEVDSLHKQLTTYKLKVRALLELIKQFNYGGEGLENNESFYQKLISTIAQDDDVEELKSQIHNLQVGSDAKSKTIVKLEEQLIDLDKQLMHTKQEYTETLEYANEYIGLNDKITTCIDEMLELLLENVELSVEEKSNLKEAKKTSSEFLVAKMNALTVALEKALHNPKIKSKSDETSVLSVVAVENVDVVANSTGVDPRDLEVLEKTNVSRNDSAMDTRFEMAVEDMHEQYENFVKSIQRKLERSADLENALSTKLAQQSNILQMMNLQNRNGGTEEEDNSQHHNSPPTSLDDFGRHASIDLSTPYKEHLESLNSLVQTLKAAIADKTHELEDMKQRIQDQERLRKSEQRSKDDLQTLKEISKQKENTWEEFIGDLEQSIEHFQTEKEVLLKENDDLRQNVELLKNDNHRLKDAMHNAVKKSEVYNEQRSRITHELKTMTDELVDLKDENSALHKLAFNVEAISNTVHKQNSEFQKFQVHLLLHLDNVFKTLSKILQKKSIDQSKRKLQVLYKTNGISDAKIMQPKLESLYNFIETALESIVESYMAVILTEKDKVHLEKGHEKEMQLRIDELERKWVSERERRKLDSNAAEIQISKLETENEALREKLYNMTIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9W6</accession>
    <name>A0A8J2X9W6_ZYGB2</name>
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        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
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    </organism>
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        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
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                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
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        <subcellularLocation>
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        </subcellularLocation>
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    <keyword evidence="5" id="KW-0347">Helicase</keyword>
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    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
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        <source>
            <dbReference type="EMBL" id="CDF91163.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1326" mass="153039" checksum="6792E15B83266AD4" modified="2022-05-25" version="1">MSTTTQDSVVNNKLLPHDGPDGPDGPDGPVKKEEDVLILIPKPNSTEQLEQLIYRYRAIQHHPKENRVEMEAIERILGQVSEDQDKYMLQLQKLRSERKQNVAYDDVLLMKQMLALQLLDKDMDIPEDLLEHSISFDSASGKSTSQLNPIKVSLDFEENAKRLSLHDKFTNAFPTKLGAEGTESIISSQIAKRISELERLPANLGTYSLEDALEFVTKDDIPSSIDAMKIRALIELKGLKLLTKQKSLRQKLISNVTSQAHHTIPYLRDSPITMAAQRSVQVRSKVIVPQTVRLAEELERQQLLEKRKKERNMHLKKVHRIIEYVNNSQLNNWTYRDRCLQFGKHAQAMHNQIERDEQRKVERNAKQRLAALKSNDEEAYLKLLDQTKDTRITQLLRQTNSFLDSLAQAVRVQQNEAKMMRGEEVPPMTDEEREKVDYYEVAHRVKEKVDKQPSILVGGELKEYQVRGLEWMVSLYNNHLNGILADEMGLGKTIQSISLISYLFEVKKEPGPFLVMVPLSTITNWTLEFEKWAPSLRTVVYKGTPNQRRGLQHQVRSGNFDVLLTTYEYIIKDRSVLSKHDWAHMIIDEGHRMKNAQSKLSYTISHYYRTKNRLILTGTPLQNNLPELWALLNFVLPKIFNSAKTFEEWFNTPFANTGAQEKLELTEEETLLIIRRLHKVLRPFLLRRLKKEVEKDLPDKVEKVIKCKSSGLQQQLYQQMLKHNALFVGAGTEGATKGGIKGLNNKIMQLRKICNHPFVFDEVEGVINPSRQNTPLLYRVAGKFELLDRVLPKFKASGHRVLMFFQMTQVMDIMEDFLRLRDHKYMRLDGATKTEDRQEMLKEFNAENSEYFCFLLSTRAGGLGLNLQTADTVIIFDTDWNPHQDLQAQDRAHRIGQKNEVRILRLITTDSVEEVILERAMQKLDIDGKVIQAGKFDNKSTAEEQEAFLRRLIEGETNRDEDDKAELDDDELNEILARSADEKVLFNEIDRQRVQEEWQEAKEQGLKEPLPRLIEVDELPEIFTEDISTHLETEPVAVGRVRERKQVFYDDGLTEEQFLQAVEDENSTLEEAIRKRREARAKRQGKHTNQEGGQEENGGLVEDPAEEPAEAITADNTQHDDTDEPFEPDEDVDGPKINPKQRSRSRRKRSSSVAVIDDDAVDSVEVKANDDAPEPEKKKPRLKIKLNLKKTPPTSDNGENNDKKLDNKSKKSKKQEINELVSRVNTLLAEMREQVDETDGHPLTSIFEKLPSKKEYPDYYAVIEKPVALDIIIRNAKKGNYKSFDDVKRDLQQMYNNARFYNEEGSWVYNDATSLENFTNKWFDTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9Y7</accession>
    <name>A0A8J2X9Y7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="20">ZYBA0S03-06678g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">BN860_06678g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Actin patch</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X9Y7"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007121">
        <property type="term" value="P:bipolar cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000601">
        <property type="term" value="P:positive regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01378">
        <property type="entry name" value="MYSc_Myo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11858">
        <property type="entry name" value="SH3_Myosin-I_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000015">
        <property type="entry name" value="Myosin I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000004">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000007">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035535">
        <property type="entry name" value="Fungal_myosin-I_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036072">
        <property type="entry name" value="MYSc_Myo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF837">
        <property type="entry name" value="MYOSIN-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 15" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10 15" id="KW-0518">Myosin</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 14" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Myosin motor" evidence="18">
        <location>
            <begin position="36"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="19">
        <location>
            <begin position="771"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="1118"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="15">
        <location>
            <begin position="588"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="948"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1242"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1025"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1052"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="1103"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1246"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="129"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CDF88994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1262" mass="141520" checksum="A89BC046B21D6391" modified="2022-05-25" version="1">MAIIKKGARTKTAAAPQKRSAKIKKAAFDTTKRKEVGVSDLTLLSTISDEAINDNLKKRFKNGSIYTYIDHVLISVNPFRDLGIYTDAVLESYRGKNRLEAPPHVFAISEAMYYNMKAYNENQCVIISGESGAGKTEAAKRIMQYIAAASSTHTQSIGKIKDMVLATNPLLESFGCAKTLRNNNSSRHGKYLEIRFNSLFEPIAGNITNYLLEKQRVVSQIKNERNFHIFYQFTKGASENYRQIYGVQKPEQYVYTSKAGCTTVDTIDDKKDFQATLKAMQVIGLTQEEQDEIFRVLAAILWIGNITFGENDEGNAEVRDTSVTDFVAYLLQIDSQLLIKSLVERIMETNHGMRRGSVYHVPLNTVQATAVRDALAKALYNNLFDWIVDRVNQSLQAYPGAEKSIGILDIYGFEIFEHNSFEQICINYVNEKLQQIFIQLTLKQEQEEYAREQIKWTPIKYFDNKVVCDLIEGRRPPGIFAMMNDSVATAHADPSAADQAFAGRLNMFASNPHFEMRSSKFVIKHYAGDVTYDITGITDKNKDQLQKDLVELMGTSENIFLKTIFPDTSDKTSKRRPPTAGDKIIKSANELVETLSKAQPSYIRTIKPNQTKSPNDYDDQQVLHQVKYLGLQENVRIRRAGFAYRQVFDKFVERFYLLSPRLSYAGEYTWDGDVLEAVKMILADASIPQKEYQLGVTSVFIKTPETLFALEHMRDKFWYNMAARIQRAWRRFNQKRVDAAIKIQRAARERNTGNPYEKLRDQGTKLLGGKKERRTMSLLGYRAFVGDYLGTNERKSKGAYIKRQAGIKEKTLFSMYGEALRTKFGRSAQRVKKTFILTSKYFYIVGQVRQQNTLHYAVEYKIDVNSIRSVSLTNLQDNWVAINLVNNQQPDPFINTVFKTELVTRLKKLNNNIQIKIGPTIEYHKKPRKVHVVRCDINDTAPLRGDTYKSSRIYVRRGHPANSTSNKKPKHVGGYVRTTIKKRRSVRKPVPPTSRTVKKPAPPPPKQINPANLAASAAQTAYNPNGNSSKSKSSGTTTSQQTSARPARRTTRTTYTTSTRSSNSQQGTSNRTSQQSSAPAPPTKRASASNYEVAPQRNSKASSPPPPPPPPPMAKAAPSEPLYEAAYDFPGSGSPSELPLAKGDVVYITRNEPSGWSLAKTFDGSKEGWVPTAYVTEHVGGVSTVSSTTTQAASSTPAATFAQSTVQETTQEDAPVEQEAVAQQANFSNGLASALAARANKMRLDSDEEGGANDEDEDDDDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XA32</accession>
    <name>A0A8J2XA32_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ZYBA0S03-08856g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_08856g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mutarotase converts alpha-aldose to the beta-anomer. It is active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and lactose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>UDP-alpha-D-glucose = UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:22168"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="5.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the C-terminal section; belongs to the aldose epimerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">In the N-terminal section; belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XA32"/>
    <dbReference type="OrthoDB" id="9402762at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05247">
        <property type="entry name" value="UDP_G4E_1_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018052">
        <property type="entry name" value="Ald1_epimerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005886">
        <property type="entry name" value="UDP_G4E"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01179">
        <property type="entry name" value="galE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007956">
        <property type="entry name" value="PRK10675.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725">
        <property type="entry name" value="UDP-GLUCOSE 4-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725:SF47">
        <property type="entry name" value="UDP-GLUCOSE 4-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00545">
        <property type="entry name" value="ALDOSE_1_EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="10">
        <location>
            <begin position="28"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF89087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="693" mass="76603" checksum="B8ABA7850C9A908A" modified="2022-05-25" version="1">MHPLVQHISFLQVAYCSMPAVTTKRKVLVTGGAGYIGSHAVVELVEGGYECVVVDNLSNSSYESLARVQVSTQKYIPFYQVDLVDRQALEQVFRDHREIDCVIHFAGLKAVGESAHIPLKYYQNNVNGTLVLLELMEKFHVWKLVFSSSATVYGDYTRYPGMLQVPEECPLQPTNPYGHTKCTIEDILRDVYASVPSTAPWSFAILRYFNPIGAHPSGLIGEDPIGIPNNLLPFMAQVAVGRRKQLSVFGNDYDTRDGTPIRDYIHVVDLAKGHVAALQYLQKNGQVCREWNLGTGRGSTVLEMYVAFKKACGIDIPMEIVGRRPGDVPLLVAKADKAKCDLEWHTELDIRQACEDLWRWVTKNPFGYSQRGVVSTVCNPGIYDTRLVTLGHGSKFQVVFGNIGASIVDIRVDGQSVVLDGGASAGNYAGATIGRYANRIMGGSFTLDKNYVLSTNDGPNTNHSSISSYHTKRFLGPLVKTPSRGIFVAEYVLLDEQPTEFESDVLVNVIYSVDVESKTLDIQYSAILVRGGATAINLTNHTYFNLNKVNSESLQGTEIRVATNSFLKGNIISKADIATFHEGEPTILGKNMPSFDHCFVLSESALLDSTKSELYPILRASHPESHVNLEVLTTEPSFQLYTGDSLNGLFPPRSGFAVEPGRYVDAVNNDRWKEAVTLAPGNIYRSRIVYRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XA83</accession>
    <name>A0A8J2XA83_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ZYBA0S11-02498g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_02498g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316464">
        <property type="protein sequence ID" value="CDF91422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5600252at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008186">
        <property type="term" value="F:ATP-dependent activity, acting on RNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022613">
        <property type="term" value="P:ribonucleoprotein complex biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23827">
        <property type="entry name" value="RWD_YLR419W-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001214">
        <property type="entry name" value="DExH-box ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056328">
        <property type="entry name" value="DSRM_DHX29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059023">
        <property type="entry name" value="RNA_hel_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF267">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE YLR419W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24385">
        <property type="entry name" value="DSRM_DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26026">
        <property type="entry name" value="RNA_hel_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="7">
        <location>
            <begin position="425"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="611"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="846"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="546"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF91422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1425" mass="161982" checksum="D9FC70DA7FF82430" modified="2022-05-25" version="1">MGKKGKAPSKDSAKSSPAPEGLPSGKKKTKGKREELSEDEKSKRQANRAKVTSSSSWTGKLPHTLLHEMCQRRKWNKVDYDMKKIGDKGMLAIAILSYTDPKTKEVLTIRMFDPTYNKISGEGAMAPQETTMEARHLAATVALYRISYNTNLHMMLPPNHKNLWYDLDDFRKSLMIENPHKAKKLFDVDPFKTLVEEQKQLQQKAKNAEAKDRQAEKQQTATVLTSLDLKHRKPETKRKEKTIKKPEHTSKVSFPRRAWEHAAFVDFEESLRQNIESSIRLHINWSSKMINAKDIKSPERNVLKQKLLSLNFRPPHVEEALQYKDPLSFLLFHLPEDDLPAYFHKRKEDSKNILEISALPLEIRNKIDRLTESGISREEAACALENCCMDEAEAAGKLTAAIVPHLVNAVEPFTSERESLEIWDQELESLESMYPEEVQSLKAGSCYTIDIIKEFKIKLKVYRTKNYPSTLPGIIVSTFERNYRLPNYIKQKVLQNLLQYITDSHLLGDMLVFNIYLWLQDHLKEIIENPGPLLSERELLASMKNDEKATSNWSTSNDNRSRRKNHTSNLSAQELASLKKEYSERVITSQYKTMLEARKRLPAWKKQDVIVDLVKSHDVTLITGETGSGKSTQVVQFLLDTLQRENDDFGKTKIICTQPRRISAIGLAERVSDERCVTCGNEVGYVIRGVNKTSSQTRIRFMTTGVLVRILQTDSSFLKNSILLIDEVHERSVDTDLIVVLLKNLMGKIQGLKIVLMSATVNVDVFKNFFNGLGTCHIEGRTFPIKDYFLDDILEIVDFKIRADKFRNYSGYDIDDKNEYIRPGADSKFFRTGQINYDLVCDVVNHVDQELEMKGNNGSVIVFLPGVAEINRCCSMLKEKDDLNRMVVLPLHSALTPEDQKRVFRTYSSKRKVVVSTNIAETSITISDCVATIDTGRAKTMLYNANDNTTRLAESFISKAEVSQRRGRAGRVREGVSYKLFSKRLYQEDMIAMPIPEIKRVPLESLYISVKSMGIRDVNKFLATGLDPPPMKSLERASGMLATIGLLNEYDNSLTELGRFISLMPVMDSKHGKLLIYSIIFGVTDVGILLASILSIGTMPFLNDVKVRDKVKALLQKFEFRGDLLAVTEILQQYLQLEDTSSKNKFLKEHYLSYNKVKDIMSTRTQFYSILKDVGFIPIKSQAETTRHLNRNSNNVAILKAVLTGAFYPHVARVQLPDPKYMATSVGAIEKDPEAKAIKYWIRNEEYIDRLQERKTNTKLSDDARDELPARRAFIHPSSILFSSNGSPNSEELATLEDVDEANMKKTFGSVTVAKFPFVVFNSSQVTSKLFLREITPTTTLSLLLFGGPISYEVNGSEHSPGIVLDSWLPIRTWCKNGVLIKELRTLLDQAIRDKLENPNYSGTNKNVEKPNAVLSLMERLVHVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XB80</accession>
    <name>A0A8J2XB80_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ZYBA0S05-07052g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_07052g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XB80"/>
    <dbReference type="OrthoDB" id="432719at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF32">
        <property type="entry name" value="COPPER RESISTANCE P-TYPE ATPASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01229">
        <property type="entry name" value="COF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="438"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="482"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="534"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="567"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="726"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="767"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1119"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1152"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="11">
        <location>
            <begin position="173"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="11">
        <location>
            <begin position="252"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF90020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1194" mass="132111" checksum="E5330EE0CC09D6C1" modified="2022-05-25" version="1">MSTLKIQIENIHCSQCEKYLASLLSQFFNLPSYLRGNVLSNRGEESWQLQKAQSIVRNRKFEKLPVTAYRVDRNAGLVTIGYVGGKDSASLCHKVKLSLADAGFAVVDAYLLGDLESLQSKTIERKSKNILSWVGNTFFGNRDMGRQRDRTHLEYCEYCRSHKTNSGQEASKFHAKLAIEGMTCASCTQSIKRIIKEHVPGTETIALDLLSGIAKFTVEDRQMLQSIVESIRDSGYQAKIIEVVPIIQQSRYKIVAAIGGITCAACASAITNAVQGLPFVEEVAINVVSKTGIFILDSNDASKLHELKGTIEDTGFDLEQIGPIELVDHASIKKVCRNVILRVDGMYCLHCPERVNSVLKNYRRAKIDIQSPITLKNPFIRFSYFPNVEEDITIRSIINSLMKEISLNSDNNFKITIVQELTLDEHLKKAAKKQVMHILIRLTATTLIAIPTFVFGVVGMSLLQNKNGFRRWLNEPLWHGEVARMVWILFILSTLVYFFIDDIFHRKALKEVRALWLQKNNWKRRILKFGSMNLLMSLGTSVAYFASVALLAIGACKRKEHNRMPDNTTYFDSVVFLTFFLLIGRLLECFSKSKSAAAITNLSSMKQNSAKLLEKHRSGQISERIVDIKYLEIGDLVKISPGDSPPADCIITDGETQFDESALTGESIPVAHFSGELVFTGTINVGRNTVMAKLISQEGDSLLDQIVNTVRDGQLKKAPVEKLADALTGYFVPIIVFLAVITWVIWLSLGYSRKLPRTYLDKDLGGWAVWSLEFAISVFVVACPCGIGLAAPTALFVGSGLAAKHGILVKGGGAAFQECSQTSVVCFDKTGTLTKGSVPDITDYSIHGNSKIRAIVPGLVRDLEANSRHPLAVGVSKFIADHFRPNGSNKIPVTEEVAGKGMMGEILSDTLDGPWSELNPERIILGNERFMSDNQVSLTSDHLRLLNHWKVQGKSVIIVAISSPTFFQKTGYFPVMLMAARDEMRPEAKDVIRTLQSWGLECWMISGDNELTARSIAKELGINQVVAEVLPHEKAEKVQWIQQTYKRHGKPAVVAMVGDGINDAPAMATADVGIALTSGSDLAVTSSDFVLLSTTQTLKALLILFQLSKKVFRRVRFNFAWALVYNMIAVPIAAGVIYPYKHARLSPVWASAAMAMSSVSVILSSLALKLYKPGGVNLSKEDITPTNVVEHRFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XB93</accession>
    <name>A0A8J2XB93_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Aconitate hydratase, mitochondrial</fullName>
            <shortName evidence="14">Aconitase</shortName>
            <ecNumber evidence="14">4.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BN860_07426g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="14">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.-" evidence="14"/>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XB93"/>
    <dbReference type="OrthoDB" id="2224430at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01584">
        <property type="entry name" value="AcnA_Mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000002">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000003">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000004">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1060.10:FF:000001">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1060.10">
        <property type="entry name" value="Aconitase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050926">
        <property type="entry name" value="Aconitase/IPM_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015932">
        <property type="entry name" value="Aconitase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006248">
        <property type="entry name" value="Aconitase_mito-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01340">
        <property type="entry name" value="aconitase_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005558">
        <property type="entry name" value="PRK07229.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160">
        <property type="entry name" value="ACONITATE HYDRATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160:SF3">
        <property type="entry name" value="ACONITATE HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9 14" id="KW-0408">Iron</keyword>
    <keyword evidence="10 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="15">
        <location>
            <begin position="63"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="16">
        <location>
            <begin position="579"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CDF90035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="779" mass="85348" checksum="3F7EBCE5E888149D" modified="2022-05-25" version="1">MLSARAALRRPLNRGLATVSGLTRDSKVHQNLLEDHSFINYANNLKNVDIVKKRLGRPLTYAEKILYGHLDKPHEQEIERGVSYLKLRPDRVACQDATAQMAILQFMSANLPEVAKPLTVHCDHLIQAQVGGEKDLARAKSLNKEVYDFLATATAKYNMGFWKPGSGIIHQIVLENYAFPGALIIGTDSHTPNAGGLGQLAIGVGGADAVDVMSDLAWELKAPKILGVKLTGRMSGWASPKDIILKLAGITTVKGGTGKIVEYFGEGVDTFSATGMGTICNMGAEIGATTSVFPFNKSMVEYLDATRRGKIAEFAQLYKNDLLSADEGSEYDEVIEINLSDLEPYVNGPFTPDLATPISKMKEVAVENKWPLEVKVGLIGSCTNSSYEDMTRAASIVKDAAAHGLKAKSMFTVTPGSEQVRATIARDGQLETFDEFGGVVLANACGPCIGQWDRQDIKKGDKNTIVSSFNRNFTSRNDGNPETHSFVASPDLVVAFAIAGDLRFNPLTDKLKDKDGNEFLLKPPTGIGLPPKGYDQGENTYQAPPKDRHQVEVKISPTSERLQVLKPFPPWDGKDAKNMPILIKAFGKTTTDHISMAGPWLKYRGHLENISNNYMIGAINAENKKANSVRNVYTGEYKGVPDTARDYRDNGIKWVVIGDENFGEGSSREHAALEPRFLGGFAIITKSFARIHETNLKKQGLLPLNFKNTADYNRINPTDKIDILGLTTFAPGKDLTMRVHPVEGKPWDAPLTHTFNAEQIEWFKAGSALNKIKQDKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XE31</accession>
    <name>A0A8J2XE31_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_02454g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316465">
        <property type="protein sequence ID" value="CDF91592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XE31"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000017">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004762">
        <property type="entry name" value="Amino_acid_permease_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00913">
        <property type="entry name" value="2A0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF7">
        <property type="entry name" value="LEU_VAL_ILE AMINO-ACID PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="330"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="386"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="432"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="456"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="504"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="545"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="10">
        <location>
            <begin position="100"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF91592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="613" mass="68718" checksum="D88BB64CB31B9832" modified="2022-05-25" version="1">MSSIKSEGHFGVTPDTIETPVDEDKGFSQTENLSECPVEVSNIEQEGIDGPRRGFFHKGVVGRVVDSYRRAQVSPGHDPEAEMMSTFSNGGLKQALKTRHVVMMSLGTGIGTGLLVANGKDISNAGPAPLIIGYGMVSFCTYFMVQAAGEMAVAFPTLPGSFNAYTSSFISRPFGFATTWLYFVQWLTMFPLELIAMTMTIRFWNTSIDSDIFVLIFYCFIFLVHIIGVEAYGETEFIFNMCKILMITGFVIFAIVLTAGGAGDSGYIGAKYWHNPGAFVSHTSEAQFKGVCYVLVSAYFSYGGTELFVLSVNEQENPRKTTPTATKTTIYRILFVYLLTMILLGFTVPNNHPRLMGSPYAKPGVQSVSPYVLAAYIHKVRILPHFINTVVMLSVVSMSSSAMYAGPRLICSLAQQGYCPKWLDYIDRRGRPTRGLILCFIVGVIGYAAHDPNREVVFGWLASVAGLSELFTWASIMLSHVRFRMAMRKQGKDLNELGYKANTGVWGSCFGVFFSILVFMAQFWVALSPPLSDGVCSANDFFQSYMAMPIWLFFYFGYMLYYRDFTFLTPLDKIDLSYHRRFYDPELLRQEDEENRQALKNAPFWVRMHAFFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T032</accession>
    <name>A0A8J2T032_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_02542g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_02542g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="CDF87267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="10256906at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056915">
        <property type="entry name" value="Ig_TR130_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056916">
        <property type="entry name" value="NTS_TR130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045126">
        <property type="entry name" value="TRAPPC10/Trs130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022233">
        <property type="entry name" value="TRAPPC10/Trs130_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056913">
        <property type="entry name" value="TRAPPC10/Trs130_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13251">
        <property type="entry name" value="EPILEPSY HOLOPROSENCEPHALY CANDIDATE 1/TMEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13251:SF3">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig_TR130_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TRAPPC10_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="TRAPPC10/Trs130 N-terminal" evidence="5">
        <location>
            <begin position="7"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Trs130 NTS" evidence="7">
        <location>
            <begin position="302"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Trs130 second Ig-like" evidence="6">
        <location>
            <begin position="670"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="TRAPPC10/Trs130 C-terminal" evidence="4">
        <location>
            <begin position="971"/>
            <end position="1078"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF12584"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF23036"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF24966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1090" mass="126025" checksum="8AA726548D9371C4" modified="2022-05-25" version="1">MSLQYSSRAVTISYFDPFDVFESIKGEFLQLLPFRNIHWKAPGGSVRTVRQLPVNLVVESEASELGVDDVNLFVRFIVVNCISVDDYRGKVRPLLKQWLPVSEDMPLEDDAVEMPKPIVLLYANSEVVDSNLFKSISLIDKFAKDFPGLKTLELKSVYKSPVDKGEFWTQMGNHLKSCLLDIFQRRLSHYRRKLSISNNTDEELSLQEKILELYLALNMHEESTAQLNKIRDNVVSKRQDSLPDGELETPFNFSGNENLDDRSIAKMLLENKLTKFQYFKYFFVKKFQLLNMGKTNVSRLLQLYKLIRGFLHSIDAIFRDSTHLLEFKYYFIENALDCINSGNLPIFMEMKAELLLLKRDCWIQGVLANTDYHLLGKEFPNVDTHYLFSRLKDTFINEDIFHENFFKFTSQLVSLYNRYDGKRQRIVDVLSLEIGMLHYQRGEYDRAVSLFDSCYEYYMESNWDVIALQIIKMFYQSLVHCKEIKFLKRDEEEASTSSVLSNAFLDIMRITGDDEEKEQAWNNFLSLQKDMDESPVYGTERLFDIKTSDFTYQSEPNVYRIDVTIRNLALPGSINADSVKLIMKSSLDEFIEFSAKNVTIFPASNNYSLEAFEISYGQFQPVSLEINAGNATFSLEFLEEVKFIFVEPLNQAGNVSCAVQQSKLLYLGEYAIDLIQKNVKDLQSLEVELIVEPNNFSYPISFSSDSNSYSKTITDIRDCTTLQYFSNEPITSFELKAKSTFYKKDNPRKFSETRVFTISCYLPVSVSVEDIFKKDAFIFKFLLNSSTAEEPIILYTSKLKATNDSARYKWFGDYEPNDFIYLTADPAESSLNCYQITTDDKFDPNDMFELQVTYNTMKEQLDGLVTDCILVQGDVVWYSEFELWKILWETKILPLLQYDYNAFMQSCMVLVLPDSLDVAKTYNSLVKLNVPIMVSRRILGSLKKIIEGVELSDIDIRAYAKNLTPRKLVVPVELPEMDHFFHVEFVKKHSEQYRVGTPMLFTIKVENLSQKWGSQGISGAFVLEIASSNEWLAHGRKRMLIRSEASEFELYIIPLKKGYLNFPRVEISNVANGESSRIDNPNASETILVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T1B6</accession>
    <name>A0A8J2T1B6_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein mS41</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein FYV4, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_17678g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS41 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T1B6"/>
    <dbReference type="OrthoDB" id="18595at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039603">
        <property type="entry name" value="Ribosomal_mS41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019083">
        <property type="entry name" value="SAM_Ribosomal_mS41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28235">
        <property type="entry name" value="PROTEIN FYV4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28235:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09597">
        <property type="entry name" value="SAM_Ribosomal_mS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01238">
        <property type="entry name" value="IGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small ribosomal subunit protein mS41 SAM" evidence="6">
        <location>
            <begin position="33"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF87929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="123" mass="14413" checksum="179EBDC2D81CB937" modified="2022-05-25" version="1">MFSRRMVSTTTALLRSQSAAKKIPSPTPDIPDVKTFLTRIGRKCEELTDVYENNWENLFTWDGKSLKDKGVSVQQRRYILHQVEMMRQHKPVEEIKLGKKSFFGGERSQRENIAKWRAEQRNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T1J9</accession>
    <name>A0A8J2T1J9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BN860_19086g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_19086g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T1J9"/>
    <dbReference type="OrthoDB" id="2264294at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001080">
        <property type="term" value="P:nitrogen catabolite activation of transcription from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN DAL81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="725"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="149"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="56"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="106"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="262"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="555"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="860"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="912"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="285"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="559"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="860"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="885"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="946"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1005"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1023"/>
            <end position="1032"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF87989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1032" mass="114983" checksum="0FA3D9A3BA7783B7" modified="2022-05-25" version="1">MSIDSERQETGDMNGDKNQNFNDKNNSNHEMLNFNDNYALILQKLANDNSHILNQDLSPHAMGSGPMTFGGEKQHSENSGNTGDKQNLDALLQHYQSLLTRSATAQYPGSSAGTSLGDNHNSSNNNKQENASNGNHSHNSNKSTITERPCDHCRRRQTKCVLVPDLADCIQCETKGIKCTFSDMPNNVNRFSGSNDMPNGSSKHPRQETGIDLEGLLKRAKTSNNDNVAQYYAELLQNLNDSMNNNNNNNVHGGFNINSSGSAVSPATVPNNGRVMNVMPPQNAPPSQRQPYHTQPSGQYPRSSLYVGATSVYDLNLVNHVKLDNIDQIQLSPSVSLRKVAPDVLFVLRDNFNEQVFLKQEQEVDMVERLIYPHGKVLIDIFFKLIHPCFPILHERVFLEKYARSYRELTAPLLASIYSLSIQWWDFHPKLIGFPKPDVVNQLNEIAYRTFFQMIERPKLSIVQTGLLMLQCRSECPNNWFLCSALVALCEELGLGVDCQDWRLPRWERGLRRRLAWAVWVQEKWTALIESRNSHLILGRNWMVKMLSEEDFPQNSPVISGSQDSISKSSSGSENPLGNISLIDMSPTDEDFLSGNLMFQQLVSLSVILGEIMDTFYTEGAVKVTTKIEQVLKLAKPLQLKLREWYHSLPSQLSMNKFVPRKFNPNATLTLAYFAAEITLHRKIISTLKQDTPKELVQVCRTAAKTRLVAAIEFVRDLKSEHINAFWYTCSTGNLMLIAIFAALLYVIAQNRDEAIIVRDYLRNYIWILRVASKSFDRSGTALKRIHILLSQIPGLLTDEHPKEFIPTRSQSPMYPPQHWKMSSMMSVPSSMDSQQMSPNLNQLRNIPAELLQTLAGMQGSSPKFSVSRSTAQSSPTNMGGEVNANASSPNYSGGNDSETAIKANLERTNIFPKSNHESGGTEQPTPDPLIESSGASNAEKKDMSDQTAINQNPKGKPITPQSVGGANVTKDSTPTAKSEAPDNSTNTSGSSVSPTSMQDKEEQSGNSNEPDLNHNSPEQGSSEEKDDQKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T1Z1</accession>
    <name>A0A8J2T1Z1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_05160g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T1Z1"/>
    <dbReference type="OrthoDB" id="1666796at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF111">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035342099" description="Transmembrane 9 superfamily member" evidence="7">
        <location>
            <begin position="19"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="422"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="458"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="505"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="544"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="579"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="611"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="651" mass="73817" checksum="615B722757A8FD98" modified="2022-05-25" version="1">MMSSTFFLVCISASLAKAFYLPGVAPTTYHSNDEIQLLVNHLTPSIYFQHEDEDGHMVKGDKEHYLYSYDYYYSKFHFCRPDKVEKQPESLGSIIFGDRIYNSPFKLNMLQEMECIPLCKTKIPGEDAKFINKLIKNGFLQNWLIDGLPAGRQVHDTRTDTDFYGTGFPLGFVDVTQGFSSSQDSGASKNVIGNVEVPYLANHFDINIEYHDRGQDNYRVVGVTVSPLSIKRSSPETCQYNAETESLTLSESEDNEVYFTYSVKFTKSDTVWATRWDKYLHVYDPTIQWFSLINCSIVVVALSSVVLHMLLRALKNDLARYNELNLDNEFHEDSGWKLSHGDVFRMPERSLSLSVLVGSGVQLFLMIACSILFAALGFLSPSSRGSLPTVMFILYALFGFVGSYASMSIYKFFKGPYWKVNMLLTPLLVPGGILMSIVLLNFFLVFVHSSGAIPAGTLFAIIVLWFVFSIPLSFAGSLIAHKRCHLDEHPTKTNQIARQIPAQPWYLRTIPVSLIAGVFPFASIAVELYFIYTSLWFNKIYYMFGFLLFSFLLLTFTTALVTILTTYHSLCLENWKWQWRSFIVGGVGCAFYVFIHSILFTKFKLGGFTTVILYLGYSTLISVLCCIVTGSIGFISSMFFIRKIYSSIKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2G9</accession>
    <name>A0A8J2T2G9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">BN860_06480g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_06480g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2G9"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00727">
        <property type="entry name" value="ISP4_OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="420"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="493"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="569"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="596"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="620"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="683"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="746"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="794"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="823"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF87443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="890" mass="101508" checksum="469A8412192E25C1" modified="2022-05-25" version="1">MDKSEIHLEDQEVTPSDLEKNEDRMRIGTVDYSDDKKETVIETASLESIPSVKVSHPQLVILLKKLGYEDPEDVTEIPPEVEYVADKISSMSLQEASDVVKMGVKYHKDDPNLSWEEYHEYEKLAALDPSTLNADEQDALNLLAVAGILNFHSPYKAVRACVDPRDDTEAPVETFRSYVLALIWGVIGAGFNEFFAHRLLTIAINTAMVQLLLFPMGNLWAKYVPCWSFPVWKGRRFHLNIPVPWSPKEQMFATLLFAISVSAFYMDSVILTIKMYYKESINFGYQFWISVAVQYLGFGFAGCLRRFVIYPSRAIWPTQLQTMALNKALFSRSEEKNTRGFTSQTFFFLCIIFIFFYQWVPSYLFQALSTFNWMTWIKPDNFDLAMVTGSMSGVGINPIASFDWNVINMYSCIVTPLFSYSNQMLGGIIAAVCVIAIFYSNQYDCQYLPMFSNSLFTNTGEEYDVTQILNKENKVDLHKYQQYSPPYYSAGNFFCYGAFIASYPMLFVYSFMTQWGVLKGAFRDWGHAIWALTRKETWLDSWRNDSHVLDQFHDPHSRMMRRYKEVPEWWYYLVLVISVVIGIATIEGYHTETPVWSLFMAIGINAVFLIPITILEATTAVQMGLNVLVEIIMGYALPGKPHALMIIKAFGYNIDGQADSYVGNLKLGHYAKIPPVALFRGQMFMVLIQVLVSLGVLNWSISNISDYCKPDQASNFTCPDATTYYNSSVLWGAIGPKRIFEGVYPILKWCWLMGALIGLGFGAWKVFAPRFFPTWFNPLVVVGGMINMAPPYNLTYMTPGAIANVFSQFYMRRYKFRLWQKYNYVLSAGFSSGLVISSIIIFFSVQYKEKDLSWWGNNVINAGIDAAGPAKRNISATPRGFFGPDFGHFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2P9</accession>
    <name>A0A8J2T2P9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_07910g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_07910g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2P9"/>
    <dbReference type="OrthoDB" id="783096at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005519">
        <property type="term" value="F:cytoskeletal regulatory protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1540">
        <property type="entry name" value="Actin interacting protein 3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022782">
        <property type="entry name" value="AIP3-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005613">
        <property type="entry name" value="AIP3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056279">
        <property type="entry name" value="Aip3p_Bud6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051825">
        <property type="entry name" value="SRCIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741:SF10">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN CG32809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741">
        <property type="entry name" value="P140CAP/SNIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03915">
        <property type="entry name" value="AIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23153">
        <property type="entry name" value="Aip3p_Bud6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00806">
        <property type="entry name" value="AIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Actin interacting protein 3 C-terminal" evidence="3">
        <location>
            <begin position="399"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="145"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="330"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="529"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="159"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="177"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="203"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="232"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="339"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF87505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="843" mass="93442" checksum="1754094A2B43D12C" modified="2022-05-25" version="1">MSSTRDGFEYSHGHHSSAPSRKSSSSILSNSSVETTVTKLLMSTKQLLQTLTQWSKGALNEKMVSDAYVQLGNDFKLVSKFFSHSGLEVSDLGDVPMDLRRVLEVALREEPSDETLNKYLPSIREIIVTLLDKLKVKQALLKSAKSDKNAKRSLHQQRPSTVSSLSLSNKSTPPSSRTPSVNYTPKSQGQEFAESRTHRKNGSVSSISARNASLSSPVTPSHPQGPPILPPISTTVAGSKQQNLSETEALSELKKGNNLQRRASKRFSAYQMAKLTNHSTTEAAAAGALASVPPSLSDVDTPSLPASSVGSIAPPLPSELMAKTLESPTEKIGTQTASIHDDQDHKDGTRGHELSAAASVPVATSHKPPRFEPNTSFKATEEKGIDENDGSSDKLYTIFLKIGDKTKRCCLQLPTSMQELKLLFVEKFAYSPGEESFPDLYLRDPKFSVFYELDEQALSDLCDGCLIKIQDKDHEGKKEVRDGKTGRQGVESKSDYSELVSLLKSEIAKSQEDILAQIKDISIKAQDSSRKTEQLSASETEDKELSSDHIAAPPVPAISVEVVKEMKQELATLRQLHNDNKRKSEALIALVISKVASLKILSLETNKPPTKTYIESTQSKLSEASDALLSKVDDLQDIIEVLRKDVAIHGVKPSKKKLDSVQKELIVAQHSLANMQAYMKNEKPQWKKIWKSELEKVCEEQQFLTLQEDLAADLKEDLDKALETFDLVNLCCAEQEKHPKKSKSNPILPIPEPGTFHKVRDQVLTEVQALHPDHNERLEALERAQKMWEKEREYRDVDAFEDELGNFVEKGNLKTSGGVEELERKRKQKDEENLRAYFGGLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2W4</accession>
    <name>A0A8J2T2W4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">5-aminolevulinate synthase</fullName>
            <ecNumber evidence="12">2.3.1.37</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">5-aminolevulinic acid synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Delta-ALA synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Delta-aminolevulinate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_14686g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the synthesis of 5-aminolevulinate (ALA) from succinyl-CoA and glycine, the first and rate-limiting step in heme biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>succinyl-CoA + glycine + H(+) = 5-aminolevulinate + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:12921"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="2.3.1.37"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 12">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 11">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.37" evidence="12"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2W4"/>
    <dbReference type="OrthoDB" id="10263824at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00375"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003870">
        <property type="term" value="F:5-aminolevulinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06454">
        <property type="entry name" value="KBL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000006">
        <property type="entry name" value="5-aminolevulinate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010961">
        <property type="entry name" value="4pyrrol_synth_NH2levulA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01821">
        <property type="entry name" value="5aminolev_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF102">
        <property type="entry name" value="2-AMINO-3-KETOBUTYRATE COENZYME A LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="13">
        <location>
            <begin position="128"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU910713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF87796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="556" mass="61211" checksum="C455FD89F7EEC6F2" modified="2022-05-25" version="1">MESIARQSVKMCPFMQRAGSVQGLQVLHNANLPAVARKCPVVGHAMSRAYATASGTGARRATAPEPKAGSANEMVANHATQESLFDYDGLFETEIEKKRLDKSYRFFNNINRLAKEFPKAHRDLEDDKVTVWCSNDYLALSKNQQVIDVMKKTLDKYGAGAGGTRNIAGHNRHALKLEAEVAALHKKEGALVFSSCYVANDAVLSLLGQKIKDLVIFSDELNHASMIVGIKHANTTKHIFRHNDYDQLEEMLQMYPRSTPKLIAFESVYSMSGSVADIDRICTLAEKYGALTFLDEVHAVGLYGPHGAGVAEHCDFEAHRLAGIKSLPDKSVMDRVDMITGTLGKSFGTVGGYVAASQRLIDWFRSYAPGFIFTTSLPPSVMAGAAEAIRYQRSHLELRQLQQLHTGYVKQGMKELGIPVIPNPSHIVPVLVGNPDLTKQASDLLMKNHKIYVQAINFPTVARGTERLRITPTPDHTDDLSDILLDAVDDVFNELQLPRVRDWESQGGILGVGESDFVEQSNLWTEEQLSLSNNQLNPNVSDPVLGNLEVSSGIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2W8</accession>
    <name>A0A8J2T2W8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_18580g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2W8"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF118">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="50"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="226"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="572"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF87967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="683" mass="76088" checksum="5C72235056F30DD7" modified="2022-05-25" version="1">MKKNKRCSSRHLPVEHISVLNHSQSTDTYRDWHCDTMLTLPVYAHHKEILQHIHANRTTVLIGETGSGKSTQLPQFLLREYRVAVTQPRRVAAINLATRVAHEQRTPLGHTVGYSVRFDHRAGRHTRCKYLTDGMLLRELLHDRLLSQYDIVVIDEAHERTVLTDLILGFLKELQHGPRPELRVVIMSATLSADTFSAFFDDCPVLHVRGRRFPVTQLYLRGDCDDVVDTAVRCCVQINAGEPLGDVLCFLPGQEEIDKAVAVVVQVAGELQRLGHPLMVPLPLYASLPPDQQAKVFQPLRKSLHQAKRKGPLRKVVFCTNVAETSVTVPGVRYVVDSGLRKVKVWRHALNLSTLLTVPVSQASAQQRAGRAGREAPGKCFRLYMEADLASMPQLTEPEIARCDVTAPLLLLKRCGVEDVVRWSWFEHPGRESVVAGLGELYALGALDSAGHITPLGERMSQLPLAPQLSAVLLAAEERGCLEAALDIVAALSVENLLLNAPPEKRDEVHERRAALCHHAQHWGDLVMCKELLDCYADVPRDEKREWCSELYVNHRAVREVAKVRGQLRRFFGEDTSGTDPEDESGAGSGAAGPAAHTPQTKLSSEQIPLLLQSFLRGFPRNVAVSHPDRSYRTASTGESVVPHPASTLFARRNCPAILYTEYVYTSRGYARNVSRLELAWLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T313</accession>
    <name>A0A8J2T313_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="15">BN860_19306g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_19306g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-glyceraldehyde + ATP = D-glyceraldehyde 3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13941"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.28"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>dihydroxyacetone + ATP = dihydroxyacetone phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15773"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16016"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.29"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Polyol metabolism; glycerol fermentation; glycerone phosphate from glycerol (oxidative route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dihydroxyacetone kinase (DAK) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T313"/>
    <dbReference type="OrthoDB" id="1724672at2759"/>
    <dbReference type="UniPathway" id="UPA00617">
        <property type="reaction ID" value="UER00669"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004371">
        <property type="term" value="F:glycerone kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050354">
        <property type="term" value="F:triokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019588">
        <property type="term" value="P:anaerobic glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061610">
        <property type="term" value="P:glycerol to glycerone phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10440:FF:000002">
        <property type="entry name" value="Dihydroxyacetone kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.340:FF:000001">
        <property type="entry name" value="Dihydroxyacetone kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1180.20:FF:000001">
        <property type="entry name" value="Dihydroxyacetone kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10440">
        <property type="entry name" value="Dihydroxyacetone kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1180.20">
        <property type="entry name" value="Dihydroxyacetone kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012734">
        <property type="entry name" value="DhaK_ATP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004006">
        <property type="entry name" value="DhaK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004007">
        <property type="entry name" value="DhaL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036117">
        <property type="entry name" value="DhaL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050861">
        <property type="entry name" value="Dihydroxyacetone_Kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02361">
        <property type="entry name" value="dak_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28629">
        <property type="entry name" value="TRIOKINASE/FMN CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28629:SF4">
        <property type="entry name" value="TRIOKINASE_FMN CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02733">
        <property type="entry name" value="Dak1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02734">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01120">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82549">
        <property type="entry name" value="DAK1/DegV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101473">
        <property type="entry name" value="DhaL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51481">
        <property type="entry name" value="DHAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51480">
        <property type="entry name" value="DHAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DhaK" evidence="14">
        <location>
            <begin position="8"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="DhaL" evidence="13">
        <location>
            <begin position="384"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-hemiaminal-histidine intermediate" evidence="11">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="58"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR612734-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR612734-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF87998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="591" mass="61940" checksum="FD228BC6856D2B7E" modified="2022-05-25" version="1">MSHKQFKSDGNIVAPYLLGLARSNPGLTVIEHDRVVFRTASAPGAGSPPKVTLVSGGGSGHEPTHAGFVGEGALDAIAAGAIFASPSTKQIFSAIKAVESPKGTLIIVKNYTGDIIHFGLAAERAKAAGMNIELVAVGDDVSVGKKKGSLVGRRGLGATVLVHKIAGAAAAHGLELAEVAKVAQSVVDNSATIAVSLDHCTVPGHKMENILGAGEYEIGMGIHNEPGTQKCSPLPSIPEVVEKMLPLVLGEDEDRAFVKFEPSDDVVLMVNNMGGMSNLEIGYATEVVSEHLIKKYHIVPKRTISGTFITALNGPGFGITLMNASKAGSDIIKFFDYPTTASGWNQTYHNSRDWDVLAEGKVPTAPPLKHLKNHKRCGVKADYDTFAKILKAGIARINEVEPKVTWYDTIAGDGDCGTTLVSGGEALSKAIDEHSLRLEDGALGIEDIAYIVEDSMGGTSGGLYSIYLSALAKGIRESGDSQLTANTFKKASKSALDALYKYTRARPGFRTLIDALEPFVKTLNAGEGPRAAAKAAYDGAEKTKKMDALVGRASYVSKEEMSKLDDEGGLPDPGAVGLAALLDALVTAAGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T355</accession>
    <name>A0A8J2T355_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BN860_10110g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_10110g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RENT3 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T355"/>
    <dbReference type="OrthoDB" id="18087at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12455">
        <property type="entry name" value="RRM_like_Smg4_UPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039722">
        <property type="entry name" value="Upf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005120">
        <property type="entry name" value="UPF3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112:SF0">
        <property type="entry name" value="FI21285P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112">
        <property type="entry name" value="UPF3 REGULATOR OF NONSENSE TRANSCRIPTS-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03467">
        <property type="entry name" value="Smg4_UPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF3" evidence="6">
        <location>
            <begin position="60"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="236"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="280"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF87599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="354" mass="40717" checksum="4C3ACBBBBF113B49" modified="2022-05-25" version="1">MDEFPKAPKKTFYRHGRGNGKKSEKRTQEGDKLDSKPLHKAGSKPRNRRRDRGPKRPLPGVKLVLRLLPPTLTQDQLLETLEPVVGNFSQWHLLRWYYVQGYYTSKIFTQPVYSRCYFIFESMDYLKKFAELVEPLVFIDDKDNSAKAVLKVSSYCESYDDEFHKPSRTSAALEGTLENDIFFQTFLKSMKLMEETPEYSYRDINLLKPFEKELDKQKGLEQAIKKRSDMALVALAGEGKPKKGKKKKKSVQGNDVKGVKGAKDNKDNKDVKDSKDVKETKRKKVKEKKTKKRDSQPKSSNNNVVIIEAAGKKELQRRKKEVQGQAKLPEKPKAVKKSAKKQVQLLKKETSEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3K0</accession>
    <name>A0A8J2T3K0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15">Phosphatidylinositol 3-kinase VPS34</fullName>
            <ecNumber evidence="4 15">2.7.1.137</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">BN860_13476g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12709"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.137"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:12710"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.137" evidence="4 15"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3K0"/>
    <dbReference type="OrthoDB" id="67688at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08397">
        <property type="entry name" value="C2_PI3K_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00870">
        <property type="entry name" value="PI3Ka_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00896">
        <property type="entry name" value="PI3Kc_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000016">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.70:FF:000019">
        <property type="entry name" value="Phosphatidylinositol 3-kinase VPS34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057756">
        <property type="entry name" value="PI3-kinase_type3/VPS34_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008290">
        <property type="entry name" value="PI3K_Vps34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF7">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE CATALYTIC SUBUNIT TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000587">
        <property type="entry name" value="PI3K_Vps34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00142">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51547">
        <property type="entry name" value="C2_PI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2 PI3K-type" evidence="18">
        <location>
            <begin position="14"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="17">
        <location>
            <begin position="292"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="16">
        <location>
            <begin position="588"/>
            <end position="854"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CDF87744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="870" mass="100473" checksum="1C14A4C9CC1AC093" modified="2022-05-25" version="1">MPSKSVTFCVSQDLETPLRIKVKSLEGHKPLLKHSQRISQPLLTQRASNVFPVSEMLVSVQVFDEERNRNMTIPLFTPYVPFKNTRKWEEWLILPININQLNTSSWLRIIIWEFEGTKSVPFFILNTPIFNIEDNTLKRGQECLQFRYDDPQSGQKLENSALLESLNKFYLGDVRKISWLDEITVPMLTKRADKKILELGTFQLNIEFPVFELPVVFAQRTYTDVQKNIPTLHNFEQSNEVAEYNKTESRMKISTGNKYNSTLKFYDPDQFSNNPIEEKYRRLERASKRSNMDRHAKPDTKKRDYLNKLINYPPGTRLTAHEKGSIWKYRYYLVNNKKALKKLLQSTNLTEETERTEVLELMDSWAEIDIDDAIELLGPDYKNISVRAYAVNRLKKASDKELELYLLQLVQAVSFECLSTFSDKSNSEFTIVDIALSQTMDNSTSMTAQQQKMINSTNNSGESTVDESAIVISPLAEFLIRRAIMSPRLGNFFYWYMKSDSEDNPYLNQIIESFWSRLPNNRVAMLEDQIVLVDMLRDLCEEIKRLKDTTIKKMDLLHHLLNTRLKHLLKNREISLPIDPDIIVVDVLPEMSKVFKSSLSPLKLAFKTKTGSCYSLMYKVGDDLRQDQLVVQIISLMNELLMNENVDLKLIPYKILATGPQEGAIQFIPNDTMANILSVHHGILPYLRSHYPDDTAELGVQDWVMDNFVKSCAGYCVITYILGVGDRHLDNLLITPDGHFFHADFGYILGQDPKPFPPLMKLPPQIIEAFGGAESSNYNKFRSYCFVVYSILRRNAGLILNLFELMKTSNIPDIRIDPDGAVLKVKERFNLDMSEEEATVHFQNLINDSVNALLPIVIDHLHNLAQYWRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3N0</accession>
    <name>A0A8J2T3N0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BN860_14532g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_14532g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3N0"/>
    <dbReference type="OrthoDB" id="648285at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000119">
        <property type="entry name" value="MFS monosaccharide transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF73">
        <property type="entry name" value="METABOLITE TRANSPORT PROTEIN YDL199C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="450"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="107"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF87789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="677" mass="74881" checksum="708ECAA51B697E0B" modified="2022-05-25" version="1">MVYEMESYNMDDVGENPFSDPAASEQGEVNEDDGDMLATGSVRSNDTAASFDLEDDLADVAVDTPHSSLPHEGPESNVEQQDGMPSYSEDIMPKDSVLTGRALLYFTSIFVSIGVFLFGYDQGVMSGCLTGHYFRDHFHNPSRAAIGVMVSILEVGALCSSLMVSRLGEKWGRRRTIKYGSLVFVIGGLFQAFAGQIGHLIAGRLISGLGVGLLSTIVPIYQSEISPPHNRGKLACIEFTGNIVGYATSVWIDYGCSYFENDASWRVPLFLQSVIGFTLFCGSFVIVETPRWLLNHDRDVEGYVVIANLYSNGDINHQKAKEEYQSIKETVLISRMQGEGRSYIKVFKRYRKRMLLAMSSQMFAQLNGINVISYYAPLVFEQAGWVGRKAILMTGVNGIMYIISTLPPWILVDKWGRKPMLMTGSIFMGFALVFISLSMLANIAATPKLVVIFVVIFNSSFGFSWGPIPWLFPVEIAPLSARSAMASASTATNWLCNWLVGIMTPILEEKITWGLYLLHATSCFSSFVAVYKVYPETAGVRLEDMDTIFNDKLPNFGISKSGSDANNTVAESEMITAKNNADSDPFKSPDDEVNFKSNQSTMSRTTAASSIQAGTIETPSMEQILDFKRQQKGQGLRTFIRRGSQGVSRLLHRVLPTKNRNNGAYDTIWDNDLEHGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3Q1</accession>
    <name>A0A8J2T3Q1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_12376g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_12376g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3Q1"/>
    <dbReference type="OrthoDB" id="7042322at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000080">
        <property type="entry name" value="Aminotransferase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42858">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42858:SF1">
        <property type="entry name" value="LD15494P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="2">
        <location>
            <begin position="112"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF88529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="450" mass="50618" checksum="527C7FE7DEA6CD13" modified="2022-05-25" version="1">MSQDRPAAINFFKGHPSFRLLPGKEVALAAEELLTPTRRDFDDDTKNRHPLTYGSDEGALWVRQVICDFNNDETFRFATTDKARSKPEFLNLNSGASYGVLSILLESTLAHTGYTRQAFVITPTYFLINDCFIDAGFSNKITAIDELGGGEIDLEGLEIKLSELEEEHKGEDDSTDLQLISNPLKPYHKKIYRYVLYCIPTYANPSGETYTLETRQKLIEIARKYDMLILADDVYDMLNYDQPLNRLAQPPKRLVHLDRETNNDPESFGNTISNATFSKLIAPGLRFGYHESVTQKLAYQLSQGGANTSGGTPSQMNSMIVGTMLKNKSAHKVLNNLRAVYRERSSALYDAVKTYLPEGTHMEPLHGGYFAWVTLPQGYNAKVIGEELQKQYSVVLANGSNFEVIGDTRNWGQRSVRLSISFLEAEEIVKGVKLWGCVCKAFAKRNDLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3Q9</accession>
    <name>A0A8J2T3Q9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein HIR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_08636g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for replication-independent chromatin assembly and for the periodic repression of histone gene transcription during the cell cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the WD repeat HIR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3Q9"/>
    <dbReference type="OrthoDB" id="1741719at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000417">
        <property type="term" value="C:HIR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000290">
        <property type="entry name" value="Protein HIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001073">
        <property type="entry name" value="Protein HIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055410">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031120">
        <property type="entry name" value="HIR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011494">
        <property type="entry name" value="HIRA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019015">
        <property type="entry name" value="HIRA_B_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13831">
        <property type="entry name" value="MEMBER OF THE HIR1 FAMILY OF WD-REPEAT PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13831:SF0">
        <property type="entry name" value="PROTEIN HIRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24105">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07569">
        <property type="entry name" value="Hira"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09453">
        <property type="entry name" value="HIRA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0678">Repressor</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="13"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="CAF1B/HIR1 beta-propeller" evidence="14">
        <location>
            <begin position="20"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="73"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="131"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="173"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Protein HIRA-like C-terminal" evidence="13">
        <location>
            <begin position="713"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF24105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF87536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="819" mass="92011" checksum="B9EAEFDB37C6CF81" modified="2022-05-25" version="1">MRIVKFPWLAHKEERRKYEVYTVDVSPDGKRVATGGLDGKIRIWSIDSIRQAAEEEGSKRTVDEELRKPLASMSRHTGSVTCVKFSPDGKYLVSGSDDRIVLIWTKDEEQRSEPIFGSEYEKEHWMVRRRLVAHDNDIQDICWAPDSSILVTVGLDRSIIIWSGSTFEKIKRFDVHQSLVKGVVFDPANKYFATASDDRTMKVFRYHKAGELAFTIEHVVTEPFKGSPLTTYFRRPSWSPDGQHIAVPNATNGPVSSVVIVNRANWDTNISLIGHDAPTEVARFNPRLFEGQQIGNSNTDGDENQKNQRDEVESIVATAGQDKSLALWSTSRARPIFVAYDICHKSITDMAWTPLGDTLFISSLDSSITIILFNKDELGKPIPLEKNIEQLHRYGVDKDSLDFPESVKQLSLEDEARKWKPQPKAQGGFLESRVAIKQADRPKKNKELSATTKNENVNILIPKRKKESKLNQAVVKNGRKRVTPTLLSSGYSPAKEASSFDKPMAGVAKPIASAAKKKSLSLTMQGIEGKLSFQSFPMPRLGIHTLIMGMKERDKDIFYKETDGATQSQTDGDGLSSGLEGAFQSADINMDKEDYSMTMNAKLGQNKIWSEEPNVRFLNTPGVLPDTDAVLVECGNFDDFCLLEIRNGVERSIQFDAEALLDNPTRILGYNKGRRTIELFIPEVVICAIGSLKSKTWCLATANGSIYFISLEGQYRLPKVSLGYKVIKFVSQDEYLVALTERGLFFVWDLHTLKLLHKNISVLPILCGEPVEGSKVRSNKRIKNFSLSSDARSLILEVTNPNSAYEWLGQMGCWCEYKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3T0</accession>
    <name>A0A8J2T3T0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BN860_17898g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_17898g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3T0"/>
    <dbReference type="OrthoDB" id="29851at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000010">
        <property type="entry name" value="heat shock 70 kDa protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF32">
        <property type="entry name" value="HEAT SHOCK PROTEIN PDR13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="463"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="474"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF87939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="542" mass="58683" checksum="94C652F0C787FE79" modified="2022-05-25" version="1">MSSPIIGITFGNTTSSIAYINPKNDVDVIANQDGERAIPSALSYVGADEYHGGQALQQLIRNPKNTIINFRDFIGLPFAEADVSKCANGAPAVNVDGKVGFVISRGEGKQEKITVDEVVSRHLRNLKSATQDYTGSKISDIVLTVPITFTAAQKDALKAAASKVGFNVVQLINEPSSSLLAHVNKFPFEQDVNVVVADFGGTRSDAAVIAIRNGIFTILASKHDSTLGGDALDAELVEFFAKDFEKKNKSNPRANARSLAKLRHNAILTKKTLSNTQTATISIESLADGFDYHTSINRLRFELVTNKVFSRWSSFISDVISEANLDPLDISAVVLCGGVSYTPKLASNLEFLLPESVAILGPNSKDASNAPNELCASGAALQARLVSDYDFEELAAALQPSIINTPHFPNAIGLIGSQGEFHPILLPETSYPVQKRLTLKDAHGDLLVGVYEGKHHIVEKTVQPIEKQEKKEGDEEDEEWSSEEEEPEIIREKLYTPSTKLMELGIKGVTKGLELVFNINKAGVLRVTARDLKSGTVVKGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3V1</accession>
    <name>A0A8J2T3V1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BN860_10462g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_10462g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="28245at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05392">
        <property type="entry name" value="RasGAP_Neurofibromin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF142">
        <property type="entry name" value="NEUROFIBROMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GAP" evidence="4">
        <location>
            <begin position="1620"/>
            <end position="1809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="315"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="405"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="532"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="790"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="933"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="412"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="424"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="543"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="798"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="829"/>
            <end position="842"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF87615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2997" mass="338165" checksum="638580FAEB8F9477" modified="2022-05-25" version="1">MGGPVRSLVKHIYWERILPLLPYETGHGQYHEVESDETFLLNRAVTMDVSYSQGLNPVVEMSVELVEHILGKQAVLQVDEKAIQSILVLVRLLSDNMEYVWDCVEARRTREVRSRGGIGSVVGFSTHRPCFHCVSPHALDSELAVKLLDLCGKIKFDTNTLHMLQNMSQQLYGTTSFVSSTVLPQYQAYLKQRNVNGNAVKIDATVDYIFRFLSAANPGEFTRYLRHTVLKPFLESHSGVEQGVLHCLDLFGCFYLNKRNLAKYLEMTRNMASGMRKTVYHCLLLYYASRALMFWIMGRSEEYTHVFQLLRRSTGSGGNSSNSSNSNSSSTNSASGATASGTGNSIISINSNEVPSSSVPGSFGTSDEDLVKPVLPLASALFEETYSTFSVSSLLTSLTSYGNTSLSGGTATEGNDSNSGTPHSASSSMSFFSQPSLNSGPSGSSSNNLADQQVLDDPSVLSSSETPSLKWGSDGRPLQPEVPHMYNVLELYTTPDGSESLSHTSVLRFLIALLMLDPDVFTEVNATTFKNLPDAKGLPTSHSRSEPEEKEKIQGSIKHITHGLKKLTTLPLSRKKSVKLISTLVKNLTGSVLIADQATMDTIKSIVSLFTMASSVSLVDPTVPSVVFCKRLFPMMACNLDLGKNWERDAKLNVSLLKCLARHPIAYGRLQIEFFAASTRLEQNTFLSRLHLSEVGSGSNLKKLTLYTEGFHVFFHLPTTKELREGTALRTAELLKTLFYWIPDVILKSLPYFDGRITTIVSSIVDGTILEEIGGNHPIFKGSSHSSSSSLASLPNSQNVALGSPQSGSAQEVYSSPTSQGGAGVVRTNSNGSSSSDSSSGNIAVNPNDAYFGHLIAPKARRVSGNSSTAKKPFSVLIAAETSDVDKGQTNLRSQGGMNATVSTSEAATISSGYTSTTVNSSMGLARNIRSPLRHTRQRRGSDDTSVGPPRSSIFTATENTISSASREHDARRIMVNIFSIFKRITDYIVVPHIENVDPEWPEKDFKSIIKSIFSSMIDTDASLQKTAQSFMDVLINYMSEFSENISPGTMCGYYKLCSYTITLFSTALLDLKLSNHNREVLLDIVVKFLKVRGRLERNFEVESTKLDSFLELERSTFPIILGTVGRALFVSLYCNRNNVQMLLKNAYKEFYQMILFHIKISGDADPSWADNLGFAKAMSEDNYVASGSVAFQRRLRLNILKYVKYPDAIMFDVMSFIYKKWSSLVGTKNMSQEESSDFRSFGGIIASVCGVLLTVDTEKYPNLSEMRSDVGRHMNFFISRQCQSLNNPDLLTRENSRDILSTELHPLAFKLLFSNLKRKLDELKGIDLTASSHELSLVLLEQIIIILRTVLRREDEDKILLLFSVDVINFIDDIVDILEKMPNDSPKFFKAVIHMSKVFRAMENAEGNLGIVGHYYLKNKWLKQVTFWFKLTIDKDYDIENISKPHREMDLKRRDIDILLIDTAIESSKALAFLTKNVPLEVPPSVSEEELKRSKFVIFGNHFNILLKGLERSTDLDSFPPSLKHKISILNENVITSLTNLSNANVDAGLQYSLPMGFSKNKNIKIAFLKVFINIVNNISPSRDQSAKAKLKAQNDLLLYAIKDPSIVVLFTKICPANEIDSFAAGLVSAFDSRNASIVLVKELIKEEIRTAPRHMDILRRNSCATRALSLFARTRGNEYLVKILRPVLTELMDTEESFEVEKLNPKDQNTDLQVDLFVKYMKLLLDSITSSVDIFPPELVLICQTIYSAVEEKFPVYVYVAVGSFVFLRFFCPALVSPESENIVEISESPYKRPFITLAKVIQSLANGSDSVVKWPALESQEEFLEECRDRIFHFLRDICEKKYKMDISINMDPTPIPFDFNYLHTYLYLYDLDVRKATLDTFQTFSDFPRIKEQFLLVDEKLAILGQPKEQFRNEIPKDIVDNKEECPQLYEFMSRHAFKSVAIFNTNSTFVHESMSPDGLPIITLTFSAFRTLNIEVETVIYRTFRIYARIWTVKHYVVLDCTGFDENEVDVPKLTSLFNNLLPPIALENCQGYYYFNITESFINQWSKLFSKLNPYVAYKVPHYFVNSYSNSDFVSSLGLSAHSMEVVNDVRVSLHDIVLYDERKKRFTPVSLKIGNKYFQVLHETIKQYKIQGWDHLVDLKFNDVFQINHVSSVGISASTGVPSEFTVGLEGGRTLIFSSAKYLEIIKMFYYALARIEDEYGVIHMDSSFETGNNDSNKNEYEQRLKTVCHLLLIVVVGLFNEDDVVKNVSYNLLAATQDAFELDFGTRFHSSPEIHVPSDCGTFLCLISKSLSKNSPDLTRYVFEFFVNGLTNQIIPFDYIPQTIFFLSYWVPNLYKHVYLVDEEEGPEVMAQIIRSLIRLTISEPSFTAVYLQQVWFYLALEGCLTFLIVEEVLNHALDRDSENRDWKELLCLLNGLPTIEVASQIINRLMNIIKSILPSLKLESFTQSWSELTILVKATIPLFFEAPLVAQMFLPEALFITSLLIDVGPTEIRSSLYELLMNVCHSLTINEALPDANKQKLDEVCNTFSRQRFRFMSGFSQHKGRVLQTFSASSFATNFAALELFATNMLHLMEYSSISESPQWKTRYKKYLMDAVFNSDSFLSARAMMILGVMGKTNTTENLCKNFLDETMKIVADPHLTEELIFLLIAHVFTYSKVVEGLDPSSEMMKQLFWLSSVFVESPNPALFEGGLFFMANCLSRLYKYHFDNNHNGQLLTSILIKARYFAEAHLVELEEYNDGRWDERNFAHNIIVIISRGCSIPSVKGTALEFLLCQFRNCHREHKTHQKSTHYLCYMFFLYLFSTPENFRSIIQEAGCADDLIALDNNNLLPKRLLNWLSSNKSCPNITLYQGAVVFNSTVSDEPCKMRFALVMRHLSKVNPECVFNFYVATREELRRISALDKTSDCVPIAFDILRLIVKTPEVSELEKYNERSLASIRERGLGFMTKIDVFDQGASNVLDILEVHSDMIYRRKRAITIILSRMSSCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3W3</accession>
    <name>A0A8J2T3W3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BN860_06744g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_06744g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3W3"/>
    <dbReference type="OrthoDB" id="413313at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013946">
        <property type="entry name" value="NCA2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28234">
        <property type="entry name" value="NUCLEAR CONTROL OF ATPASE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28234:SF1">
        <property type="entry name" value="NUCLEAR CONTROL OF ATPASE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08637">
        <property type="entry name" value="NCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF87454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="495" mass="56725" checksum="82E528D4A8A1C488" modified="2022-05-25" version="1">MITDKYVLEALDSLATQGPPAKELEPLVHEVRRAIRQGKYQPLQLPQLDQLSSQDPLLLNYVALLCYYILTNQCLLQLPPAYEAARYFKAAKSTRHNQMLYSLQTAPERLWKCLPGLPTAWPRSDLRKRYNECERFARHYTAALGRLMVEFRASDKLALEDSLEQKLKALRTFLKLDSANMAQIVQETQNSAVASSTTAYRVPSLATRYWPVALLVLCYGPTSLVALWHSRRAIQRFLQENVVDFARGLLYNWVYVPLKQVWATVRHDEDSSIAVMSKGTLDSESSSLTRMVVRFVRENSSQSFDEDKLVSQVEHGDLTEFLEIYENQLEKPVRNLITGALVRSLLIQLQKTKVDGSLALSGIDRMLQSQQLVFGVVALSPALLIIYTTSVSAYRLCKVGKLWSYEKQHREILSQSLNNAERLLNYDDEQKEIYYNQGLLAIEVTTVFNMGLTLVPRSRWPEWTRDVAEIADSKLSLQSRLNVITRIYHTYGKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3Y2</accession>
    <name>A0A8J2T3Y2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Nuclear fusion protein KAR5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">Karyogamy protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_18646g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 13">Required for nuclear membrane fusion during karyogamy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Nucleus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the KAR5 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3Y2"/>
    <dbReference type="OrthoDB" id="5311848at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000742">
        <property type="term" value="P:karyogamy involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048288">
        <property type="term" value="P:nuclear membrane fusion involved in karyogamy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007292">
        <property type="entry name" value="Nuclear_fusion_Kar5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28012">
        <property type="entry name" value="NUCLEAR FUSION PROTEIN KAR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28012:SF1">
        <property type="entry name" value="NUCLEAR FUSION PROTEIN KAR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04163">
        <property type="entry name" value="Tht1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 13" id="KW-0415">Karyogamy</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13 14" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035205723" description="Nuclear fusion protein KAR5" evidence="14">
        <location>
            <begin position="19"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="451"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="476"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF87969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="509" mass="58697" checksum="539651CB5EFED2AA" modified="2022-05-25" version="1">MLVLLFVLWSTIISVVHCGSAAALANRLQERVLLSETKQDILSDELLQRSFPFLKSSCVKEALKHFLPACLKQGVESIDASFRVETAVKLSICEFQASGLKHIPEACQKNGPDSLMDCMIQLESSTQWWTTYSGNYQRLSSICFENSLPFEKDQILSLFLNITNMQVEISDNLSKYFYNMMSNVESSAESHLKTIIELFNEYMNQFQEVFKDKRNVFKNEFEEFQEDIQSVMVHNADLLKEKDIGFMQSVDSLQKIIDGILIQLEENDISSQIIQLRNDNLHDVKEMERLTQEMFKFQIQGQNQMGYEMKRFFDDTLQNMALVSHEVEKSQATAAEVTQVFDQFKSSLIPSIMKELSPQLLGLKSDLLEDWKSAMKIVSRDFTLWNSQINESFEEISSRLNSTMETINDIDRSFSNLKNIMTGITRIFGYLGIFGHSTFRVLCYIMHNKHVWIFTAILIFVKQLTFWLPWRKLLSLFVSYGQFMVKCGIIAIAIYSGSKFGKTIMSINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3Y3</accession>
    <name>A0A8J2T3Y3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_18470g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_18470g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3Y3"/>
    <dbReference type="OrthoDB" id="5835136at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001401">
        <property type="term" value="C:SAM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019564">
        <property type="entry name" value="Sam37/metaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031317">
        <property type="entry name" value="Tom37_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10568">
        <property type="entry name" value="Tom37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11801">
        <property type="entry name" value="Tom37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mitochondrial outer membrane transport complex Sam37/metaxin N-terminal" evidence="1">
        <location>
            <begin position="24"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Tom37 C-terminal" evidence="2">
        <location>
            <begin position="176"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="310" mass="35890" checksum="14D21ECE14502FDD" modified="2022-05-25" version="1">MGVPIKTFYLWGEEGKPSLVSPESIALYWFLNGYYEERMSSEIVFANNTDLSPNEELPLLVNGSKRLSGFVNIVNSLAEREEDGTGANEDIEMLLLRDGLLEYTNELTVLTDYQLYLNRVNYETFTRGAFSKLLYWPMWYNTPINYRALTRQRCTDTLGYLLHDDDPDKLDDFNIDSANLAQSKTFKVSQARKMRSKEELQNAKHNVQFLEELTRHLEIWIQIREHIKSDKVITADLLMWANVYVQLNLPEGDRIGKHIASKLGDDFLQTLKVQLKVCSSLETKVPQRDPTFQEQGNVIMSLCNRVQKFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T405</accession>
    <name>A0A8J2T405_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_19152g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_19152g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T405"/>
    <dbReference type="OrthoDB" id="2110361at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF6">
        <property type="entry name" value="PROLINE UTILIZATION TRANS-ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="42"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="111"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="111"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="826" mass="92990" checksum="936EBC2D249F10EB" modified="2022-05-25" version="1">MQREYLSEDLSERYDTGDEDTPSGAGNNGYVKKNNAKRSSLACVRCRRRHVKCPGGDPCAKCAAAKIACEYLEPNKKLIVSMKYLQQLQESLADMKKENVQLQSIVNSLRPGERNAERNELERTASKSSQADIEDEEQKESAETPEVVSTNLTDEEEIVSNFAQRSGRLVESRTGQRYFVGSSSMTLFGLEIQSLLSKYLSHKKEGDQIPFATDNKTGGTLLKGPQASLGQIDVLQEEGNAYKIVLAKNEGNDDISVNFTLPSYSYAMFLVDTFITYNDGCFYFFNEGIVKYGLKIAYEGGAYYEDRTLQTIWFCKVLLIFAIGEMYLGTSNNINGNKVKDTAKLPGSGFFEEASQIFSCLFSSGRIESVTKEGGIEVMLLYAFYLQVADCTVASYFYFGQALRACLILGMHVDAQRDFLTRFELEHRRRLWWTVYMFERMLSSKAGLPLSFTDNTISTELPGDFDMSNPASGCEHYIFPEAEYIINCVKIVKINAQILNKLYQRQPNTNILAALKSVVRQLLQWRNNLSGFLQVEFTGKTLKISRLCANMFTEYFQGMNLAIRPLLFHFASIQLKKFKGSNTYINLQNYSSTISALLNCSLQASVNTIRSLWALMDQNMVALFGYMDREYLFTASCTLVLFNAAFGIHEQTYEHLDHALNIFTKMRNLGNNPAGLRRSQLLTLMANLDFHGIMRELISFHNDSLKPDTQYDAHNFRNVNIVSQTMTDSSSLSDIINATLGQSAQANLSKQETQIMEKNSNQFDPLNAADDGDLQALLEKMSKESHTDSKLWKEISDQAMWLGNTMDPTSATGNVTDFGEGLGLNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T429</accession>
    <name>A0A8J2T429_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Kynureninase</fullName>
            <ecNumber evidence="4 5">3.7.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Biosynthesis of nicotinic acid protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">L-kynurenine hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">BNA5</name>
        <name evidence="6" type="ORF">BN860_13828g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3-hydroxy-L-kynurenine + H2O = 3-hydroxyanthranilate + L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25143"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36559"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58125"/>
            <dbReference type="EC" id="3.7.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>L-kynurenine + H2O = anthranilate + L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16813"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="3.7.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Amino-acid degradation; L-kynurenine degradation; L-alanine and anthranilate from L-kynurenine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the kynureninase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.7.1.3" evidence="4 5"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T429"/>
    <dbReference type="OrthoDB" id="5978656at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00329"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00334">
        <property type="reaction ID" value="UER00455"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030429">
        <property type="term" value="F:kynureninase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097053">
        <property type="term" value="P:L-kynurenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000031">
        <property type="entry name" value="Kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01970">
        <property type="entry name" value="Kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010111">
        <property type="entry name" value="Kynureninase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01814">
        <property type="entry name" value="kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14084">
        <property type="entry name" value="KYNURENINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14084:SF0">
        <property type="entry name" value="KYNURENINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22580">
        <property type="entry name" value="KYNU_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038800">
        <property type="entry name" value="KYNU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4 5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="3 4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="141"/>
            <end position="144"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR038800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF87760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="443" mass="49584" checksum="F11FF887A264C679" modified="2022-05-25" version="1">MEEAQHLDSQFPAQLRGQFHIPTFASMGLGSDNRELTYLCGNSLGLMPRATKDAVDRELNAWAARGVEGHFNHPFTGDAGDATNWVDIDLPVVPLLAPIVGAQDDEVAIMNSLTANLNALLVAFYKPEGNKNKILFEKGAFPSDFYAFHEQCRLHGVDPTNALVQLEPREGEYYLRTEDILAAIDQYSNQLALVCLPGIQYYTGQLLDIARITDHAHVRGITVGWDLAHAVGNVPLQLHDWGVDFACWCSYKYLNSGPGAIGGAFVHARHSTSSLPRLAGWWGNNRERRFAMLEKFEPIDGALGFRQSNPSVIDVVCLRTSLQLFQQFGSMSQVRERSVRLTSFLKRLLKKSVYYRDQTPKSSDLGFTIITPEQESLHGAQLSLLFHSEMDKIFKHCRSRGLIADERRPNVIRLAPAPLYNTFQDVANAVQILEEALQEIFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T449</accession>
    <name>A0A8J2T449_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Vacuolar ATPase assembly protein VMA22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_17788g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T449"/>
    <dbReference type="OrthoDB" id="4044452at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990871">
        <property type="term" value="C:Vma12-Vma22 assembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040357">
        <property type="entry name" value="Vma22/CCDC115"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31996">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31996:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21730">
        <property type="entry name" value="Vma22_CCDC115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00093634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="157" mass="18212" checksum="D778ADD86AD549EE" modified="2022-05-25" version="1">MLEEEDLLELLKLLSLYDLLLEQLQKSLSEGFSLLGRANYHNKDSLRGRYGRDYWDESYEGQLMVKSGKELMISRRMGQGGTQETPKEEKSLRRRCEKPISIENTPKPRDPIYMFGGVLSTPTSLRQSQSHFKSSIPLMTELLNCRRRIEKLITTRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T482</accession>
    <name>A0A8J2T482_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">26S proteasome regulatory subunit RPN8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_14334g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M67A family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T482"/>
    <dbReference type="OrthoDB" id="10256771at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08062">
        <property type="entry name" value="MPN_RPN7_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000004">
        <property type="entry name" value="26S proteasome regulatory subunit rpn-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024969">
        <property type="entry name" value="EIF3F/CSN6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033858">
        <property type="entry name" value="MPN_RPN7_8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540:SF7">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13012">
        <property type="entry name" value="MitMem_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="6">
        <location>
            <begin position="8"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="302"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00072102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF88613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="326" mass="36777" checksum="E4416528C089F845" modified="2022-05-25" version="1">MSVPYERITVAPLVLLSVLDHYERTHTPEGKRCVGVVLGDASSPVLRVTNSFALPFEEDDKNSDVWFLDHNYIENMNNMFKKINAKEKLVGWYHSGPKLKASDLKINELFKKYTGNNPLLLIVDVKQEGVGLPTDAYFAVEQVKDDGTSTEKTFVHLPSTIEAEEAEEIGVEHLLRDTRDQAAGGLSIRLTNQLKSLQGLQRKLGDIVAYLNKVTNKELPVNHVILGKLQDVFNLLPNLGQPNEDELNLRNNEVVKHNNNLQKALTVKTNDELMVIYIANLVRAIISFDDLIENKIQNKKLQEKKNKDYEADHPSKTKEEEPVAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T497</accession>
    <name>A0A8J2T497_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14">Sulfiredoxin</fullName>
            <ecNumber evidence="4 14">1.8.98.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN860_18866g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>S-hydroxy-S-oxy-L-cysteinyl-[peroxiredoxin] + [protein]-dithiol + ATP = S-hydroxy-L-cysteinyl-[peroxiredoxin] + [protein]-disulfide + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17545"/>
            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="Rhea" id="RHEA-COMP:13681"/>
            <dbReference type="Rhea" id="RHEA-COMP:17976"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:61973"/>
            <dbReference type="ChEBI" id="CHEBI:61974"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="1.8.98.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the sulfiredoxin family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.2" evidence="4 14"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T497"/>
    <dbReference type="OrthoDB" id="10023328at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032542">
        <property type="term" value="F:sulfiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16395">
        <property type="entry name" value="Srx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1530.10:FF:000005">
        <property type="entry name" value="Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1530.10">
        <property type="entry name" value="Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, ParB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016692">
        <property type="entry name" value="Sulfiredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21348">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21348:SF2">
        <property type="entry name" value="SULFIREDOXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017267">
        <property type="entry name" value="Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="ParB/Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="7 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB-like N-terminal" evidence="17">
        <location>
            <begin position="12"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="82"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Interchain" evidence="16">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR017267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR017267-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR017267-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CDF87979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="127" mass="14244" checksum="C7B075C305969ED0" modified="2022-05-25" version="1">MSVQTGNLNRITEIPLSQIRRPIAPVLDHSKIDAMVSTLDGQPMASATCTLEQAQELKGQLPPVDVLCVREKGQTYYFAFGGCHRFQAYERRAAQEQRDVPVRSKVIPATRHQLKLYVGASVDEMFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4C6</accession>
    <name>A0A8J2T4C6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_13366g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_13366g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4C6"/>
    <dbReference type="OrthoDB" id="2919105at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031934">
        <property type="term" value="C:mating-type region heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031508">
        <property type="term" value="P:pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF15">
        <property type="entry name" value="NAD-DEPENDENT HISTONE DEACETYLASE HST4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="7">
        <location>
            <begin position="77"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="28"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="376" mass="41999" checksum="F67069182B17CD5A" modified="2022-05-25" version="1">MVHELMLNSTQNDSNDSELLPLTPPATVEKRRSDSQTKRSEVKPKKLLTQLRGSNRKSRLKFRPQPFTVLDLNDHLDGLRDDKNADFLRYALRHSRRIVVIAGAGVSVAAGIPDFRSKEGLFSSLKDKNCASGKDLFDYNQVYSSDEMSYKFNGMIANLHRLSQGAQPTAFHSMLDEIASEGRLQRLYTQNIDGLDTRMKHLNTKIPLEKPPPVTIQLHGSVNHVECNMCSKIENFDPSIFRCSSQDVDEAQVVPTCPQCEEFESVRMIAGLRSKGIGKLRPRVVLYNEIHPEGDLIGEIAASDLKKKPDCLIIVGTSLQIPGVKSLCKQLVQAIADRKGIILYMNKELPTKSLIDIIGSIDLIVLGDCQEVSKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4F9</accession>
    <name>A0A8J2T4F9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BN860_14774g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_14774g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4F9"/>
    <dbReference type="OrthoDB" id="10265668at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030627">
        <property type="term" value="F:pre-mRNA 5'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059164">
        <property type="entry name" value="HAT_PRP39_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204">
        <property type="entry name" value="PRE-MRNA PROCESSING PROTEIN PRP39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204:SF23">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN COMPONENT PRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23241">
        <property type="entry name" value="HAT_PRP39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="411"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF87800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="557" mass="65339" checksum="61FE0A7A6F7BA056" modified="2022-05-25" version="1">MDKFTRLVSDPEFSNLTLEVARFPKNLGGWEKLLNHLLAAVSPLNKSIDLKLYQLLVSTYESLLIQFPYLENYHVDFALMEYRLGHLDKVHRIFDHGLAIFNQKSLLLWISYLKICNEIVPHHKQLLKKYETAENHIGLHFFAGEFWQLYLEQVRQRCSHTQKFFSILRKVLEIPLYSFATFYAMWLRCVEDVRDLSQLGRLAPEDELFKKLKININASGRRGPRLQEAKKALKKATREMYLVVQRQVLEIFDLYESKLNTRYYVSPETLIPASEIELWQKYLTYTVNLKLDPLTHLNFQRALLPLAHYEIVWIQYARWLVDWQGDLLTAKNVLVQGVSLSSKKSKILKFLYSILCSLGEFDQLQLIIKFVETAYLDNVGETDDFELFWDYVQFKTFALRAPKDKPSRYESSNVSTGPDSSSSTASQLLPREVLDIIVQRLGQCESKDGQYLLLSSVLQLQHEGTTSTIENEIFQRLVKDNYTYYLDDGLFWSLYCRLIFLDPARSYLEKRRYIVKVVWPQAVARHKNQILPHLQEFCQAYTPEDIDSLEELFQCGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4L2</accession>
    <name>A0A8J2T4L2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_17656g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 6 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4L2"/>
    <dbReference type="OrthoDB" id="344220at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.580">
        <property type="entry name" value="Mediator complex, subunit Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007018">
        <property type="entry name" value="Mediator_Med6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016612">
        <property type="entry name" value="Mediator_Med6_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038566">
        <property type="entry name" value="Mediator_Med6_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13104">
        <property type="entry name" value="MED-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04934">
        <property type="entry name" value="Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF013286">
        <property type="entry name" value="MED6_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="56"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="61"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR013286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF87928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="263" mass="29388" checksum="B59754B6ACF2033A" modified="2022-05-25" version="1">MNTPLDELQWKSPEWIQAFGLRTDNVLDYFAESPFFNKTSNNQVIKMQRQFSQMGPALATEGSQNGPESVRQNAGTSSRDEVDFENLEPVRRDILARYPMHALLEKELSKMTGLEYVIGYIREPDFWIIKKQNRVGPSEVQILQDYYIIGANIYQSPTIFRIVQSRILSTSFHLSSTLERLQSLVEFTPSQGVQFKSPQDAMGVNASAPSTAATTGPLSTVATAPTAQATSSQLEQAASQNSITPEMMHKLMVISVKSTPEYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4M1</accession>
    <name>A0A8J2T4M1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_08878g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4M1"/>
    <dbReference type="OrthoDB" id="4046837at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF88380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="509" mass="58144" checksum="37E8452AB6145D1B" modified="2022-05-25" version="1">MLKRHFVSSSRLLKSTTQFAPVYAKLLNHECFKPLLNKLPAKYEQLDPFDLSRSINEMLANSSIAADQISMIHNKLIEELSPYEYGISTLHAKKLEQSGAELSEKSLLQIIKNNPGRVHDSWEILMQCSRDRWTDDLLVAVLENTISTKTLKENDKNFLPLKYLSQCVLLAANISNKELLNPQVLNSLLDNILDEKVTDALHIVLKCQLAPLSIFSARIDDLTPGQIYQMYKHFPLDTLAANKNLFSKVLYTLGTNKSIPLSQEEINANQELLKYLGTFLYESKLCDSLVRNENINSPNVQEDYLHLKNYIAEKRMDHHCFTLAKIILRIEGVYKDDMSQALELYHSYLLSYQNRADELMFEIFLSFASQSYKLSDPILLEYAQAFISADYTAENVSNTVRILILANCKFDVEKSLQIYNSNIETFIKREDGTLSSGAVTESLILAYLANEDLDFARVVFDGAVKERVLLEVTSKKHVKALFKLYGEAAEENKAREVMSEEIIKFFQTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4P5</accession>
    <name>A0A8J2T4P5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BN860_18734g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_18734g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SURF6 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4P5"/>
    <dbReference type="OrthoDB" id="444809at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029190">
        <property type="entry name" value="Rrp14/SURF6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029188">
        <property type="entry name" value="Rrp14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007019">
        <property type="entry name" value="SURF6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14369">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14369:SF0">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15459">
        <property type="entry name" value="RRP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04935">
        <property type="entry name" value="SURF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal RNA-processing protein 14 N-terminal" evidence="6">
        <location>
            <begin position="8"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA-processing protein 14/surfeit locus protein 6 C-terminal" evidence="5">
        <location>
            <begin position="220"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="34"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="369"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="130"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="250"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="378"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="413"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF15459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF87973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="447" mass="51703" checksum="6C90B483782A820E" modified="2022-05-25" version="1">MVNLLEERLRSNSNAFEGLLSLIPAKYYYDDQTQEQWKAKKKSKSQTKEDKHKKLDPEQKGEGSMSTLEVMKEREKNAKPVVLPGQKLKMMQQMKPEAQAGEAVKEKQQSEEVSEEAEDTEDIDVIFDDEGNRVPLKREPLQSHVEESSEDTSKIEPSKDEQLEDSSDELPDGQKMEVKHQTNKPNLEALRSKLQAKIQDMKEKRRAPGTKIKGAPSSREAILAERKRKLDFKIQKKDFLQQQQQQQQDESGESDSEDEDSDAESQPRKRSKTEVDANNVMFQNIEFDDGNKITSNLQNLRKGPKKKGPAKNDIRAHLKLVEAKNAKLQDKDEMDQVSAKEKEKWQRAVLQAEGIKLRDDEKLLRKALKRKEAKKRRSTVEWKDRQQAVKNSKAEKQKRREENLQIRKENKGVKRSKQQKMKRAYNGRSNSIKRAGFEGRLKSGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4Q7</accession>
    <name>A0A8J2T4Q7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_16358g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_16358g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4Q7"/>
    <dbReference type="OrthoDB" id="5873279at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="203" mass="22824" checksum="AE0671AF01785E2C" modified="2022-05-25" version="1">MVKIIYGHSADHFKQLKVAGDFTSWGIKPMKKNAEFWEYTFQKGSLAQDAKVIHFKFIDDNGVWFTDDDYAKETDSNNNENNVRILTREELEELGEVVPSKEQSYDMGDEGPVRPAPTPRIDDGGATPAEHESEGKSRNASQELGESTVIVNHSDAQEAENRPGTSGTVYSQAPEATQYKTVLARVIAFFSNLFRSWFSHRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4S5</accession>
    <name>A0A8J2T4S5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fatty acid synthase subunit alpha</fullName>
            <ecNumber evidence="3">1.1.1.100</ecNumber>
            <ecNumber evidence="4">2.3.1.41</ecNumber>
            <ecNumber evidence="2">2.3.1.86</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">BN860_04896g</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>acetyl-CoA + n malonyl-CoA + 2n NADPH + 4n H(+) = a long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP(+).</text>
            <dbReference type="EC" id="2.3.1.86"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. Fungal fatty acid synthetase subunit alpha family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="3"/>
    <dbReference type="EC" id="2.3.1.41" evidence="4"/>
    <dbReference type="EC" id="2.3.1.86" evidence="2"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4251012at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005835">
        <property type="term" value="C:fatty acid synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00828">
        <property type="entry name" value="elong_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08950">
        <property type="entry name" value="KR_fFAS_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.20:FF:000005">
        <property type="entry name" value="Fatty acid synthase alpha subunit FasA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2490:FF:000001">
        <property type="entry name" value="Fatty acid synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.25.70:FF:000001">
        <property type="entry name" value="Fatty acid synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="entry name" value="4'-phosphopantetheinyl transferase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00101">
        <property type="entry name" value="AcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002582">
        <property type="entry name" value="ACPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040899">
        <property type="entry name" value="Fas_alpha_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047224">
        <property type="entry name" value="FAS_alpha_su_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026025">
        <property type="entry name" value="FAS_alpha_yeast"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041550">
        <property type="entry name" value="FASI_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050830">
        <property type="entry name" value="Fungal_FAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004568">
        <property type="entry name" value="Ppantetheine-prot_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00556">
        <property type="entry name" value="pantethn_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982:SF21">
        <property type="entry name" value="FATTY ACID SYNTHASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982">
        <property type="entry name" value="MALONYL COA-ACYL CARRIER PROTEIN TRANSACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18325">
        <property type="entry name" value="Fas_alpha_ACP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18314">
        <property type="entry name" value="FAS_I_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000454">
        <property type="entry name" value="FAS_yeast_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="4'-phosphopantetheinyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 23" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="15" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-0521">NADP</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 24" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carrier" evidence="25">
        <location>
            <begin position="155"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="26">
        <location>
            <begin position="1133"/>
            <end position="1668"/>
        </location>
    </feature>
    <feature type="active site" description="For beta-ketoacyl synthase activity" evidence="22">
        <location>
            <position position="1316"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="1786"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="1787"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="1788"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="1886"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="1887"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="24">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="CDF88215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1901" mass="207276" checksum="0BA1847A5C0EF095" modified="2022-05-25" version="1">MVMKPEVEQELAHILLTELLAYQFASPVRWIETQDVFLKDLNTERVVEIGPSPTLSGMAQKTMKNKYESYDAALSLHRQVLCYAKDAREISYTPDPAELEAKEKALNENAGGDATGASSAPATSAPAAPAPAAAAAAAPVAAPSAPVAEMPDEPVKASLLLHVLVAHKLKKPLDSVPMSKTIKDLVGGKSTVQNEILGDLGKEFGATPEKPEETPLEELAETFQETFSGSLGKQSSSLISRLISSKMPGGFTLTVARKYLQSRWGLGGGRQDSTFLVALTNEPPARLSSEPDAKGFLDSMAQKYASLAGVDLSVATAGAGGAGGAGAGGAGGATIDAAAFEELNKDQRILARQQLQVLARYLKMDLDGGERKFLKEKDTLAELQSQLDFLTAELGDYFISGISTSFSRKKARAFDSSWNWAKQSLLSLYFEIIHGVLKNVDREVVSEAINIMNRSNETLIKFMEYHVSHTDINKGDNYKLVKDLGQQLIENCKQVIDMDPVYRDISKPLGPKTTIDKNGNITYSEEPKEKVRKLSEYVQEMALGGLLSKQSHSTIEEDLTRVYKAISAQAAQHDISGSTALEFEKVYGDLMKFLQNSKDIDASQTTQLAGVVDDALDKDSTKEVASLPTKSKISKTVSSTIPRETIPFLNLKKKTSNGEWQYDRQLTSLFLDGLEKAAINGVTFKDTSVLITGAGKGSIGAEVLQGLLQGGAKVIVTTSRFSKPVTDFYQALYAKYGAKGSTLVVVPFNQGSKQDVEALVEYIYDDEKNGGLGWDLDAIIPFAAIPENGIELENIGSKSEFAHRIMMTNLLRIMGNVKIQKSARGIETRPAQVILPMSPNHGIFGADGLYSESKLSLETLFNRWHSESWANQMTVCGAIIGWTRGTGLMSANNIIAEGIEKAGVRTFSQREMAFNLLGLLTPEVVQLCQKSPVMADLNGSLQFLPDLKGFTNKLRSDLIETSEIRKAVSIETALEHKVVNGDNADAAHAKVEIQPKANIQMNFPSLKPYKNIRKYSSADLEGLLDLERVIVVTGFSEVSPWGSSRTRWEMESKGEFSLEGCVEMAWIMGLIKYHNGNLKGRPYTGWVDAKTNEPVDDKDIKSKYESTILEHAGIRLIEPELFNGYNPEKKQMIQEVVVEEDLEPFETSKETAEQFKLEHGDKVDVFEIPDSGEFSVKILKGATLFIPKALRFDRLVAGQIPTGWDAKTYGVPEDIVSQVDPITLFVLVSVVEAFISSGITDPYEMYKYVHVSEVGNCSGSGMGGVSALRGMFKDRYKDQPVQNDILQESFINTMSAWVNMLLISSSGPIKTPVGACATAVESVDIGVETILSGKAKICLVGGYDDFQEEGSYEFGNMKATSDTLDEFEHGRTPSEMSRPTTTTRNGFMEAQGAGLQIIMNADLALKMGVPIYGIIGLTATATDKIGRSIPAPGKGILTTAREHHGELKFPSPLLNIKYRKRQLTNRKEQIKQWVEGELEMLGLESESVPSELQSTFYAERTEEIKREASRQLKSAQAQWGNEFYKNDPRIAPLRGALATYGLTIDDLGVASFHGTSTKANDKNESATINEMMRHLGRSEGNPVMGVFQKFLTGHPKGAAGAWMLNGAIQILNSGVVPGNRNADNVDKQLEQYEYVLYPSKSLKTDGVKAVSITSFGFGQKGAQAIVIHPDYLFAAIDQGRYEDYVARVSAREKAASKFFQSGMIHNKLFISKEHAPYTDDLEQSVYLDPLARVSKSKNGSLEYEASSLQKKDNFVTDDNKKTADIVSKITKQAVGGSQSGSGVGVDVELISSVNVTNETFMERNFTENEIAYCRAQPSAQSSFAGTWSAKEAVFKSLGVKSQGGGASLKDIEITRAKDEGPQVVLHGQAKKAAAQAGVINVKVSISHDDFQSVAVAISEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4U9</accession>
    <name>A0A8J2T4U9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_17810g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_17810g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4U9"/>
    <dbReference type="OrthoDB" id="4070688at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="368" mass="40957" checksum="A69FCAEEEA71B62F" modified="2022-05-25" version="1">MVLLPQMKSIWLDEDEEAEKLYGLQAQQFMGPDDEGSLAIRMVNSDKPLLSNKKKIDLKPLTKAKLKVPRSPVKNPRKPKSGFFSLFKGKEGPALKSKKISTPFQFQHISHADAKAGFESDDEEEQESHEEDIIHPSCSEGCNHNETTEEALLKKRVSQARMSYSGSISTSSSRISRFSSGAGRIVSTSTMATSIQNCHSRVPSLNNLSKNCVKGRNIDESDGTLDFLKNYDFPSELGHDVLFTFTPDTKPHKISTPIIEAESCYSQELSLTKKSPTLSRTKSDPQLLSTPQLETRWFEDGTPATRKSLDEVLRCYHQPSEENSPLKLVIETGSPFNFGFEEQSLHVEGSPLQLCQSLSSTTSPLKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4X9</accession>
    <name>A0A8J2T4X9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_13124g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 31 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4X9"/>
    <dbReference type="OrthoDB" id="10257739at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1340">
        <property type="entry name" value="Mediator of RNA polymerase II, submodule Med31 (Soh1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038089">
        <property type="entry name" value="Med31_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008831">
        <property type="entry name" value="Mediator_Med31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13186">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05669">
        <property type="entry name" value="Med31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0010">Activator</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="127" mass="14988" checksum="5B17E928A2FDCD80" modified="2022-05-25" version="1">MSEKPNQEASTEEEEPALPTRFEVELEFVQSLANIPYVTYLLTQQQQIWKDPKFKNYLKYLEYWCEPPYALCIVYPNCLFVLKLINGFMENSTVNEDGLLDGLEDLPKVLQMQGPQWMNEMVERWVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T521</accession>
    <name>A0A8J2T521_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="16">BN860_08900g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_08900g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T521"/>
    <dbReference type="OrthoDB" id="3981162at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903561">
        <property type="term" value="C:extracellular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030446">
        <property type="term" value="C:hyphal cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030445">
        <property type="term" value="C:yeast-form cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000128">
        <property type="term" value="P:flocculation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030448">
        <property type="term" value="P:hyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2430">
        <property type="entry name" value="Agglutinin-like protein, N-terminal domain, N2 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024672">
        <property type="entry name" value="Agglutinin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043063">
        <property type="entry name" value="Agglutinin-like_N_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033504">
        <property type="entry name" value="ALS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011252">
        <property type="entry name" value="Fibrogen-bd_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001389">
        <property type="entry name" value="Flocculin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793:SF2">
        <property type="entry name" value="AGGLUTININ-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793">
        <property type="entry name" value="ALPHA-AGGLUTININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11766">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00624">
        <property type="entry name" value="Flocculin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01056">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="Bacterial adhesins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035308839" evidence="14">
        <location>
            <begin position="22"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="domain" description="Agglutinin-like protein N-terminal" evidence="15">
        <location>
            <begin position="52"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="465"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="883"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="465"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF88381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1011" mass="106509" checksum="AD0D5AD669AD7025" modified="2022-05-25" version="1">MAALLIRLIFVICCRALIASAAEINDISFSDLDYIPLTSLRYPHQGWSASLDFSISSSLSVYSGDYFTLDFPTVYRIKFDGDRATTNITLQDGTEAFECFAAQQAAYKYQNTILKCIAIEDLSSYSSLSGSISFGLSFSNGGSIYQYELENAGQFHSGTMDLSLTGQLSAPVEFDSVNYTEGLYTIGRTTTYNGLESYYLGMNCPNGFLLGGTQTINYDRENKGYELNCSSTQIYLSDKFNDWFLPLSNDNADANVDCSENTLHITMGQANVDQKLWVNALQLVSAGVNTIQHEVHLQYSCSNTAEKTTYQSKFSTVVEYTIYQASDSGILSGVSTKSSPTSSSISTPTITSTSTTKWTGSYTSTYSTGSTELSGSTGVPTEEIIYYVETPDTQLLNSTSSVIPISSTQISTEYLTTNTITIFSTTTTAWTGTYKSTYSTVTTSVSGSSTSGLVTEEIVYYVETPSGTQNTPSMGTNSSTWSKPSTPSISSMKSMSFSPSSEVTSTINMSSSSSFATTCNCTDLVSLTAGSSTVVSYPTSESFVPALESSEQVTTSLLSSTISLPNAISPSSFTPAVSSKILESSNFSTNIASGTVSTRSVTGPSTNQTVTNISTTSTVLHSNSITSGPILLPASSSVISLASTETSSAFSARTTDVSTITENYSGVSSVESTSLASSSANTSTTAEDYKSVSRFESVSLGASSNFSLESIGASTANNTSLSTAQTFSSNVATSPTIANYSSSSAFSTFTSKLNTTATTKDVLQATDFDSYLRETSTGIPTVSQHSSIVRATSFPSALSSSDASSPAYTTTISSAVNSFSSFLTIENLTTSTSVFSDKSSVVTVPSTEASTINHRGTTFKESSSFSSLNHSFSTSATLPPANVSSSNIPMNTQSLEVNGSSSSSQARFSLGFSSDQGSMESASLPSTPYTSTVTTLAVVTVTSSTTTAVIATVTQNGSECAANAIQSSLKPYSTEFSSSSFSLVPYSAQGLLLHASNVSAFLGMLLLFLHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T584</accession>
    <name>A0A8J2T584_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_11760g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T584"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23813">
        <property type="entry name" value="UBCc_UBE2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000020">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="3"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="153" mass="17526" checksum="84F40654521372D2" modified="2022-05-25" version="1">MASLPKRIIKETERLVSDPVPGISAEPHEDNLRYFAVTIEGPKESPYEDGIFNLELFLPDDYPMEAPKVRFLTKIYHPNIDRLGRICLDVLKTNWSPALQIRTVLLSIQALLASPNPNDPLANDVAEDWIKNEEMAIRKAREWTRLYAGKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5E3</accession>
    <name>A0A8J2T5E3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_12750g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DEAH subfamily. DDX15/PRP43 sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5E3"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022613">
        <property type="term" value="P:ribonucleoprotein complex biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17973">
        <property type="entry name" value="DEXHc_DHX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000324">
        <property type="entry name" value="pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000003">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044756">
        <property type="entry name" value="DHX15_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF109">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="105"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="295"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="756"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="24"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="762"/>
            <end position="777"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF88545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="777" mass="88788" checksum="2F47E878764236EC" modified="2022-05-25" version="1">MGTKRRLSEKAEHESAVESSIPEKAAEIAEEISKKHPAQREEHLEHHDAGEFSSLVRHHTTAAQAKKLEDGEINPFTGRPFTPKYVDILKIRRELPVHAQRDEFLKIYQQNQIMVFVGETGSGKTTQIPQFVLFDEMPHLENTQVACTQPRRVAAMSVAQRVAEEMDVKLGEEVGYSIRFENKTSNKTILKYMTDGMLLREAMEDHDLKRYSCIILDEAHERTLATDILMGLLKGVVIRRPDLKLIVMSATLDAKKFQSYFHQAPLLAVPGRTYPVELYYTPEFQRDYLDSAIRTVLQIHATEDAGDILLFLTGEDEIEEATRKVQLEGDQLMREEGCGPLAVYPLYGSLPPHMQQRIFQPAPESHNGRPGRKVVISTNIAETSLTIDGIVYVVDPGFSKQKVYNPRIRVESLLVSPISKASAQQRAGRAGRTRPGKCFRLYTEEAFKKELIEQSYPEILRSNLSSTVLELKKLGIDDLVHFDFMDPPAPETMMRALEELNYLACLNDEGDLTPLGRLASQFPLDPMLAVMLIGSFEFHCSEDILTIVAMLSVPSVFIRPTKDKKRADEAKNQFAHPDGDHLTLLNVYHAFKSDEAYEYGIQKWCRENFLNYRSLAAADNIRSQLERLMIRYNLELNTTDYESPKYFDNIRKALASGFFMQVAKKRSGGKGYITVKDNQDVLVHPSTVLGHDAEWVVYNEFVLTTKNYIRTVTSVRPEWLIELAPAYYDLHNFQKGDVKFSLERVKERFDRMQEFQNDIEKHKKKGKKEGKGKHKHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5J7</accession>
    <name>A0A8J2T5J7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="14">BN860_10264g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_10264g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Bifunctional enzyme that catalyzes the last two steps of purine biosynthesis. Acts as a transformylase that incorporates a formyl group to the AMP analog AICAR (5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) to produce the intermediate formyl-AICAR (FAICAR). Also catalyzes the cyclization of FAICAR to IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(6R)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + (6S)-5,6,7,8-tetrahydrofolate</text>
            <dbReference type="Rhea" id="RHEA:22192"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="2.1.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:18445"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="EC" id="3.5.4.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PurH family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5J7"/>
    <dbReference type="OrthoDB" id="6017153at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00133"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01421">
        <property type="entry name" value="IMPCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.20:FF:000003">
        <property type="entry name" value="Bifunctional purine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000003">
        <property type="entry name" value="Bifunctional purine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.440:FF:000001">
        <property type="entry name" value="Bifunctional purine biosynthesis protein PURH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00139">
        <property type="entry name" value="PurH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024051">
        <property type="entry name" value="AICAR_Tfase_dup_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024050">
        <property type="entry name" value="AICAR_Tfase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005492">
        <property type="entry name" value="PRK07106.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00355">
        <property type="entry name" value="purH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692:SF0">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN ATIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN PURH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000414">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00798">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MGS-like" evidence="13">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF88437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="593" mass="65275" checksum="7BEEA4AFA9D8D79A" modified="2022-05-25" version="1">MSNYKRTAILSAYDKTGLLDLAKGLSEANVRILASGGTARMVREAGFPVEDVSSITHAPEMLGGRVKTLHPAVHGGILARNLEQDELDLQRQHIEKVDFVVCNLYPFKETVAKVGVTVAEAVEEIDIGGVTLLRAAAKNHARVTILSDPHDYPAFLAELKKNHGQVSQELRNRLALKAFEHTADYDAAISDFFRKQYSEGKAQLPLRYGANPHQKPAQAFVQEQEELPFKVLCGSPGYINLLDALNSWPLVKELSASLNLPAAASFKHVSPAGAAVGVPLSDVEKQVYFVSDIKNLSPLACAYARARGADRMSSFGDFIALSNIVDLPTAQIISKEVSDGIIAPGFEPEALEILKKKKGGKYCILQIDPNFVPAPMETRQVYGVSLQQKRNDAIINKSSFREIVSANKNLTEQAVIDLTVATIALKYTQSNSVCYARNGMVIGLGAGQQSRIHCTRLAGDKADNWWFRQHPRVLSIKWAKGVKRPEKSNAIDLFVTGQIPTDEPEKSEYESKFAELPTPFTDEERKEWMSKLTNVSLSSDAFFPFPDNVYRAVRSGVKYIAAPSGSVMDKAVFSAADSFKVVYVENPIRLFHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5P9</accession>
    <name>A0A8J2T5P9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BN860_11562g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_11562g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad17/RAD24 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5P9"/>
    <dbReference type="OrthoDB" id="10265971at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02019">
        <property type="entry name" value="NK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004582">
        <property type="entry name" value="Checkpoint_prot_Rad17_Rad24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057927">
        <property type="entry name" value="RAD24-like_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12172">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12172:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03215">
        <property type="entry name" value="Rad17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25812">
        <property type="entry name" value="RAD24_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Checkpoint protein RAD24-like helical bundle" evidence="9">
        <location>
            <begin position="302"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF88494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="600" mass="68561" checksum="50F36B4AD12FF68C" modified="2022-05-25" version="1">MDTHRPPGIQRSLSELSSQIKNVSPVKRRRRDKTEKRENKFEPWYMKYAPKSSNEVAVHKKKLEDVSRLFTSMLSGRDQTRIMLLTGPSGSSKSTLVSQLAKELLPRHRIGSSNARESQSSERCEDIVEYTQLGNFQDFLDEAKYRTGRNLSMVLVEDLPNVFHTDTRIAFQKALLKWLYSPEELLPPLVICLTECELENESSNGYGVDYSFTAESVLGKELLSHPKLTRIKFNPINTTLMKKHLKNVCMANRAFMSQRGRWPQKDLVVQQLASMTGDIRSAIANLQYWATSSGDVPFTTREQSLTYFHIIGKALYGSHACSDHEMVNSLMLNSKSHLSNDNFRLGLLENYCLVNGGNFGIRTAFSVIDGLSQSDLAGPLLESLEFAVRKVRHEFNTVDKTHASHSKPMFPRDWRIRQRQREFEVHCEDYTNVEFYKYNNSCSKKDLCLLLGYYGPLIRAKRNYKRNSLKHYASNLPPKAQENIMDQNNDITIVDPSIDVLERIGGAINAVDGVQDVVCTEDREKLTKHSLEHLKQLRDGKLKTLEMFQVIEEPEADAEVLQDLIEDTDVEEDDDSLYELLSQKRTTVNESLSDSDLEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5S7</accession>
    <name>A0A8J2T5S7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">E3 ubiquitin-protein ligase listerin</fullName>
            <ecNumber evidence="5 16">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">RING-type E3 ubiquitin transferase listerin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN860_13608g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">E3 ubiquitin-protein ligase component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. Mediates ubiquitination of proteins derived from mRNAs lacking stop codons (non-stop proteins) and other translation arrest products induced by poly-lysine sequences and tandem rare codons. Ubiquitination leads to CDC48 recruitment for extraction and degradation of the incomplete translation product. May indirectly play a role in chromatin function and transcription.</text>
    </comment>
    <comment type="function">
        <text evidence="16">E3 ubiquitin-protein ligase. Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the ribosome quality control complex (RQC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the LTN1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5 16"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6108at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16491">
        <property type="entry name" value="RING-CH-C4HC3_LTN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000038">
        <property type="entry name" value="E3 ubiquitin-protein ligase listerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039795">
        <property type="entry name" value="LTN1/Rkr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054477">
        <property type="entry name" value="LTN1_E3_ligase_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054476">
        <property type="entry name" value="Ltn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054478">
        <property type="entry name" value="LTN1_UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039804">
        <property type="entry name" value="RING-CH-C4HC3_LTN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12389:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE LISTERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12389">
        <property type="entry name" value="ZINC FINGER PROTEIN 294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22958">
        <property type="entry name" value="Ltn1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22999">
        <property type="entry name" value="LTN1_E3_ligase_6th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23009">
        <property type="entry name" value="UBC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01197">
        <property type="entry name" value="FANCL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 16" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 15 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="1501"/>
            <end position="1548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CDF88581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1555" mass="177993" checksum="A63DAD7B7B3102CA" modified="2022-05-25" version="1">MSFGGANTLQQYSTDYGLGHNGVKISLNYFDGIPESALLNALESNDMKLIFKSLLKRDETTKERACADLLKLLNDFKSNEYIFDNDIFSLCWSQVYAKLLMSESKNIRSQSHQITTTLIRVLNKRISKFLKDLIPLVLMGTCDTDASVTKSTKLYLSEAFKHDPKKIHTLWTAFHEQILNLVKEAIVVENEGTLSDERYVSKENSILRYHRVMTSAILLLNNLVRINRDHMGDYKSTYRAILKEDSLWKLLNLRDTNNLKTYEALLQLLNTLYSIGYMIAHKETFKMATRSLLKSLSQVTSKNVLNLVHTVPVILTVLASLDTYKVGKIWTYEKASKDKLITFLSVTTTATCPGYYKAMYALYDKSSDHELLNYNTEWFPLWRKSVHSLNNKLVLGRFGAECLSECWKCYLTFAERGNLDPELVRSDIMSTLNNGKSLSKLPALRQTLSDSVPAEYLTQKLQEFLTTKKEGKVEYRTFYLENLLMLLADAPSNERAIYELLKFVFGSISNETGAVSETRKDELEIYRFFINTKLEFLSEEIAKFVHEIPVWLDRDSYELLSGIIVDYTNSSFIDKNKETVTSLEDFFIGASAASIPTKRMIATLSKISDNAYQKLSLCDQIKSLLQEYIKSYDYSDNGELFKSHLVTEETIIRVYECAAANRRLDDFCTHISCLDSKTNKTLFEHTNILSSCLFTVTKENAGVLFKVALIEAKSSSSIAEILANTLLEHASEGSFESLDYAVKLLKVNPSVSSIFSPKNKLDLFGRNVPFIDYRFALVNGLGLNTHLLDLSIQPANLAQLRKFINYSFFLDHLITRLPDLAADGDIIFLTMVSELAVDYNCVSSVPNDSYYDFKNTVYKSALYSCGFSELIEQLLDTSDTTEPGSFIPELRRHSVHASVTLYKFRILSKILLNEAESISQASFNKHIPSIEAFVGSVIRSKKENSQDYLLVSIIFASLVKFQNSEPLTKLRTILASQCIGASTSEFVNEGYKAIILLNNLLTHGSDTANDVLPIAPQRLNMVLRTVSGLLDSDLVYQEEFGVVRLSLLSFLNLLLRFPAIKEVGSSVFEISARLLMDSLSMCQLEDTAFLLELRLYSLNLYLQLLKFDKELWVSTYDDEIHDSLTELCFINFTAEINNQISAVFYRALDKAMSTLPTKKTMPYFDQFYENFLAATNDECYINHLRLIASILAKLVSEKQQEAVIEFEFIKQQRDKSKEDENEEDLSSQETFKLPQDLVAKLTDDMPQDYLEYENQYNFIKYLWYWEICLSYFHEVSYNLRQLYIDQLRSEDLLNKILDFLSDQIDLQDTKFWTDAGPQAIVNYNFTQNEFSPYRNDIFLECKHLLGHCMFDLFNNIGSLVSGWWLNIKDKSTQSKIEKFVSQFISPISINHELEDVQSKMDRLTSKDDSLTIKINKVTNEVKASYLIDDQKLELSFKLPFNYPLTNVQVIGVSRVGISEQKWKQWIMSTQRVITGMNGSVADSLELFTKNVNLQFSGFEECAICYSILHAVDRKLPTKTCPTCNNKFHGSCLYKWFRSSGNNTCPLCRGEIPYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5S9</accession>
    <name>A0A8J2T5S9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Palmitoyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_13212g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5S9"/>
    <dbReference type="OrthoDB" id="9909019at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF43">
        <property type="entry name" value="PALMITOYLTRANSFERASE APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="12">
        <location>
            <begin position="175"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF87733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="365" mass="41718" checksum="7CD576925D2D6A38" modified="2022-05-25" version="1">MTRISRRRQDESASTSASSELSIAPMSKLQLFYLWLVTINGDPQISQGPGYRALPNVTNYMLFLGGRFRTVRHTANLSPLTGAIMVAPMVLFSVFETGRLWHTEDGYKVLVIMFYYFWTMCLVFFVRTATSDPGVLPRNIHLGQVRKNFQIPQEYYNTITLPAPQTTRGDPLATIELKYCTTCRIWRPPRTSHCSTCEACILTHDHHCIWVNNCIGQRNYRYFLVFLASAVVSSALLVANCSVHLSHHTRKPSEVPVAILLVIYAGLSLAYPLLLLLYHILLTGRQQTTREFLRQVKTKNPVFTKIRRIEHNPFDKGNFLSNMASLMLQARGISTLSPREQLAEGDWRYRNLSDFHSFEKVSPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5X8</accession>
    <name>A0A8J2T5X8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_14136g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_14136g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5X8"/>
    <dbReference type="OrthoDB" id="5584247at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052246">
        <property type="entry name" value="Cell_Polariz_PKAAnc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13155:SF1">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13155">
        <property type="entry name" value="A-KINASE ANCHOR PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="403"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="2">
        <location>
            <begin position="140"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF88604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="427" mass="49490" checksum="D943F2678F2BDE11" modified="2022-05-25" version="1">METEFAQIEKERLPTLYEVLLKKTSPPVDLWAFYTFLSQYPYAIDFLDFWIDMMAHVRLCKDYIKGVRESIEARGKIADGDNHSEESSILLDALLNEGYLDLKNTKRVSQFLRGNVEDSARVSKLLDNMRCEHQQSDSEQEVDDFLAEMVDDMLKEKTRQNKQPKITRKQLLNNAMQICSLYLISPQQSPRYLINVPDELRMETLRHVQSERRFDPQVFEELRDMAYNFLEMDCFPKFLSRVALHNLHDQLSDWRFQTIAAMGKSRHMSGKSRGHKSPSPLSTYSGIGRIALGLCWLGIGFWIGYVLIFLHYSRAIRVTTVVPFGVGCYFTVCGIYQVDILYSWFGVTQKLMYEKKLPADGEQNYSKIKCRVPSIFALLGGRGRLMPVHQPFIRKLLLKRGMWCFLLVTVLTAVFTVLFSCVPGHRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5Y7</accession>
    <name>A0A8J2T5Y7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S03-11914g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_11914g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5Y7"/>
    <dbReference type="OrthoDB" id="4033880at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16993">
        <property type="entry name" value="ENTH_Ent5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF5">
        <property type="entry name" value="EPSIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="2">
        <location>
            <begin position="25"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="343"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="374"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="400" mass="45716" checksum="DE297C96227B417C" modified="2022-05-25" version="1">MDSLSKKIQNLGIHDIRNAARFAQNMIVQYEPYQVDIRRATNTDAWGPTPKHLEKVLRNRYQVPMYLMTEYTLKRLIDHIATRPKNFYEKARKEYVNYGSEWRVVLKCLIVVEFLLLNVSDGDELNQVRSCLTTHKHILSREVLEYRVRISNDGKMEIHERGIRKKCENILQLLEDSHYLKRERGKHIKNHLKIRQQQEQSFYNANTYSASTATSDFDYVDDEGLSDDETGYEMSSEADASTAHAGRRRTTMDEQRRQRREMLRERIKNSEQQRKKQLQPQPEVPDLIDFDFDGSAPAPKSAESDLLGNGDSKPANDDDDDDFGDFQGGGANTSKAEPLTIHTTTTTSTKNTTSNLDDLFEFGEPTQSTNPVTNGASNGNKAANNGDAFADLFSYSKSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T656</accession>
    <name>A0A8J2T656_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_17502g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_17502g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIH1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T656"/>
    <dbReference type="OrthoDB" id="5135119at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097255">
        <property type="term" value="C:R2TP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050734">
        <property type="entry name" value="PIH1/Kintoun_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041441">
        <property type="entry name" value="Pih1_CS_Ascomycota"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012981">
        <property type="entry name" value="PIH1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997">
        <property type="entry name" value="PIH1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997:SF0">
        <property type="entry name" value="PIH1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08190">
        <property type="entry name" value="PIH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18482">
        <property type="entry name" value="Pih1_fungal_CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIH1 N-terminal" evidence="2">
        <location>
            <begin position="12"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Pih1 Ascomycota CS" evidence="3">
        <location>
            <begin position="245"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF87921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="330" mass="37775" checksum="6365D8B2D9BE2C33" modified="2022-05-25" version="1">MDFFLRPQSKAESVITINPKPEFVIKSKLASVQNRPLDLPFLEAGAKVFINLCHDPQVPKPDVDFDPAIVYPLIINDQWEIPIITSSVRQDKDKKGALCYVWDCCINSKCVQWIRKEYQLREIVVEWCLESCDLCELIEISRDKITFPKLKSKGSIAPLEILSEDLNNDYKKEMTKILKEDRDEPRDLIEIRGNLMDDEEKAALAGAELPPLIPRNAAAKSSKPLIEEVDALSIEDKKCKKLNDVHFQVNMSKESGNHKLRIDIISELNSAKNYEVQYNPSSNELVIKNTNLQEFNEKILTIPLPDIFVDVESQMQCSFIKSEKKLSIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T664</accession>
    <name>A0A8J2T664_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_07844g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T664"/>
    <dbReference type="OrthoDB" id="370884at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054295">
        <property type="entry name" value="CHS4-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF16">
        <property type="entry name" value="CHITIN SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03142">
        <property type="entry name" value="Chitin_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22997">
        <property type="entry name" value="CHS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="456"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1034"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1062"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1088"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase 4-like" evidence="12">
        <location>
            <begin position="365"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="27"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF89043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1164" mass="131280" checksum="35948EE8F107AB3F" modified="2022-05-25" version="1">MSYTNEDEYYLHFNQDQESLLRSRVGSFRSGSTRRQGSLVRPERNRFNDPENPHYYYAQKAQQQKDHLDIQPSTTGIVPTPSMRRTPGSFRSLNSFALPTEQSRSNLSEVPENFQMKDMSGKDPKVNDPLTEEVIDLDNEDYASEENLALAAQKSPPSDLRKKKAKPLSPWMIYCYIVTFWVPAPLMALCGMPKKERQMAWREKIGLLSVIGYVGVFVAYLTFGFTKTVCSSSKLRLRNNEVDGSYLIMNGKAFAFDSSSHPAAAGIDAGTNVLYPPINAGGKDASFLFQNVNGNCHNLINPRSNCTIPHDDDNNLAWYFPCALFAQDGSTKPNFTKPRYYDGWGCHTSKEARKSYYSLESAADVYFTWDDIKNSTRNLVVYNGHVLDLNLLDWLEKDQVTYPALFDDMRNLQLQGYDLSIVLASSHEKKVARCLTEIIKVGEVDSKTVGCLASDVVLYASLVFILSVVIMKFLVACYFRWAVARKQGAYPVDNKTMDRQLNSIEDWSENMNSQGPLKEVAPELRPGHHQRGANRQSKFDLFKKRGTKSFSTNEITNEIDGEGGGYLFADGSYRGMTTMTTQNAAKLQRGTNNGGTGKFSTRNQSAMLSSSSMMLNGTMGTSVPLPSSIESLDPSIIHVDCVQQPPLNYMPFGYPLVHTICFVTCYSEDEDGLRTTLDSLSTTDYPNSHKLIMVVCDGLIKGSGNDQTTPEIVLGMMENFVVPVDEVKPHSYVAVASGSKRHNMAKIYAGFYKYNEETVPVENQQQVPMIAIVKCGTPDEQGSAKPGNRGKRDSQIILMSFLQKMTFDERMTELEFQLLKTIWQITGLMADYYETVIMVDADTKVFPDCLTHMLAEMVKDPLIMGLCGETKIANKAQSWVTMIQVFEYYISHHQAKAFESVFGSVTCLPGCFSIYRIKSPKGPDGYWVPILSNPDIVERYSDNVTDTLHKKNLLLLGEDRFLSSLMLKTFPKRKQVFVPKAACKTIAPDEFKVLLSQRRRWINSTVHNLFELVLIRDLCGTFCFSMQFVIGIELIGTLVLPLAICFTIYVIIFSIVSKPTPIITLVLLAIILGLPGLIVVITATRWSYLGWMFIYIMALPIWNFVLPSYAYWKFDDFSWGDTRTIAGGNKGAHDDNEGEFDHSKIKMRSWREFEREERLARSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T667</accession>
    <name>A0A8J2T667_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_17832g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_17832g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T667"/>
    <dbReference type="OrthoDB" id="17948at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000081">
        <property type="entry name" value="RNase MRP subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF87936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="280" mass="30906" checksum="D601207F40483DE4" modified="2022-05-25" version="1">MLVDLNVCWPQKSFQQSLSDSEIEKVKAVLSTLHVLGYTHVALNFTVNHSDKFPSNPNPMQINEHFGELMRATGLKLYSRITLVIDDPSKGQSIAKLSQHFDIVAAQPISERGVTLATTNLDIDLLTFNYSQRLPCFLKHKSVCSCVKRGVKLEIVYSAALKDLQSRRQFVNNVRSIVRSSRSRGIVISSGAQSPLECRNILGITSLIKTLGLSSDKCSQAMSQLAASALLNGRLRNKSYKQTVALGTNVVEEMSDNCVIVKRNLAQDSGQGTKRIRTTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T669</accession>
    <name>A0A8J2T669_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S04-10220g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_10220g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T669"/>
    <dbReference type="OrthoDB" id="2117459at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06869">
        <property type="entry name" value="PX_UP2_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047168">
        <property type="entry name" value="LEC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024554">
        <property type="entry name" value="LEC1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024555">
        <property type="entry name" value="PX-associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47185">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN YPR097W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47185:SF1">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN YPR097W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12825">
        <property type="entry name" value="DUF3818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12828">
        <property type="entry name" value="PXB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="271"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1014" mass="116364" checksum="B1B6E9BE24D4B79C" modified="2022-05-25" version="1">MSIILTPTQEHYLKRELLKAQLNHEFTALNDQYALRKFGFPFSPDDPTKDGISRKKRLLRHMIPPLGDSSHGQKDDDEAAVDNYMHSDTQFPMLSYVLEEIIMPFPLLSRELAMDEEFWQKKVQVFYEHFESLGFSESYDREEFTKRKRISKKLNKAILLLFNSGIGASQEREYYEHDKFKLQDDSIKKATKAGEISMPTKENLRNYVTNEPVFINGWDVNVVAVAPEAVVFNRPDSPKKSRPTPTSPTKEKGSYYSKWMKPKFSMQPSSLLSKLSLTDTSVKPSKLDAYFLIRIKRKSDPRKIIFVAKNYEDFKKLAHQLKSEFPGKKLANVPHKTKKSISVVTTLDTQSSPVSGKTPSTPKEKIVASMPLDSPQTTSASVFSNADSDASSIADDASTDDWDFDEFQDASDAKSNRLVGERMRTSLRQYLRSLCGDEEVSQSLNLCHFFTKKPLAKESFNKELLQDIKHRELVDISNLETQVRFQKLTLKRSIELQDAMKELKTSLLKDENYLLSLVGELKVKTKVSELSPLLRSFVEWCKVYLSSTIYQVFLGNDSGYEFYTQIKRLHKLMPYTMMAQIMRFTNPMGMMKAMIDLFMAQPFGGQSLLQTMFSTILADDLKGQEKVTHELEQKALHDASGALDVIECLKKSVFKNERGQIIDMKRVHEESDSMGMPACLVILLKHAENGAIAATAVDDVIESYTAWKSLKKTPVDKIKPQQEADAAYFTRVKELLQLYVRERDKILMKKLWQDPELSQLLRSIVTLVYEPMVRIFKVARVDIALKNFERFMNDLIKLIDEIYNGQQGVSSQFNVVDSINNLFTKHQDACLTFIHEVYVHDTEGIFEGFITWIVRIVRILQKSAYGGPEERIDFSQMLQKSNVDINLLRQQVDDVIQKKQEARATYSKLLDVKIRKEEQSLSKNTGKALETKWKQMNALVLPTNGDTIGLGDGELVDLDLDASDYENLKHEDDVELEKKYKRILEREVDVSEIEKFGNEVFESKLKSLLFQNAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6L3</accession>
    <name>A0A8J2T6L3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_02608g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMC family. SMC2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6L3"/>
    <dbReference type="OrthoDB" id="10255539at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03273">
        <property type="entry name" value="ABC_SMC2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000278">
        <property type="entry name" value="Structural maintenance of chromosomes 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000385">
        <property type="entry name" value="Structural maintenance of chromosomes 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027120">
        <property type="entry name" value="Smc2_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="13">
        <location>
            <begin position="521"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="253"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="354"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="428"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="754"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="842"/>
            <end position="932"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF88112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1170" mass="133120" checksum="64AC35FC0320FAC7" modified="2022-05-25" version="1">MKIEELIIDGFKSYATRTVISDWDPQFNAITGLNGSGKSNILDAICFVLGIASMSTVRASSLQDLIYKRGQAGVTKASVTIVFSNLDPECSPIGFENSPKLSVTRQVILGGTSKYLINGHRAPQQSVLQLFQSVQLNINNPNFLIMQGKITKVLNMKPNEILSLIEEAAGTKMFEDRKEKAQRTMVKKELKLQENRTLLQEEIEPQLGKLRNEKRIFLKFQEIQSDLEKIQRIVLAYEYHSLVDQQATMKETLDTGETRMAELNNLIVKTKEEVRHLNEDMDCINAEKQKEFDKDGKMARLEEKESELMSNLSRLHASFDICVENISDTVKGLDKARLEVGKNQGDFNQKSQTYKTVETEYNQCNQRLKRLKDLLSKKSELLSTLETGISSTGNTGGGYNEQLTAAKNRLLETQMTIQKSDLKIEHLNKELNANKPKLEQTKQENMENMKQIEQYESLCNTLTEEMNKYGFDSSVVNELKSKQNHMRQELYQLTAETESLKRTVANVEFTYSKPYENFNCDSVKGVAAQLFTLSEENLDSALALQICAGGRLFNIVVSDQTTAAQLLERGKLKKRVTIIPLNKIAARTIDPERLKLAKQLAPNQVQLALDLVGYEEDVSKAMQFIFGNSLICKDAETAKKITFHPQIRTRSITLQGDVYDPEGTLSGGSRSNRSSLLLDIQKYNSATNRMKQLESEISGVSHKLLEQEALSGKTKEIQSKLSLASHKLSIFQRNLNDNPSSQIIKRSEVLAKQIEECESEIRNSRASLMEYEVEIERIRNDMEEFSRDKGTKLEELKKEITTLTKEVSKLENATDAKYDLFQNLQLETDQISSEISSNRAIVEQLSTSLESFEKQKNDINIDTQHMEHLLADVQTELNEEKRRLLDIDEELKELDRLVKSKIERKSNYELELQKLTNDIASFKNSKNSMAQRIKQLVNDSSWLIDEGLVAGIIQQNPNINLNQYKDREKFLKERYDELKRKVNTNIMTMIENVEKKEGALKTMIRTIERDKEKIQDTIFKLNEYKKETLINTWKKVTADFGGIVSDLLPNAFAKLVPCEGKEVTEGLEVKVKLGNIWKESLVELSGGQRSLIALSLIMALLQFRPAPMYILDEVDAALDLSHTQNIGHLIKTRFKGSQFIVVSLKEGMFSNANRVFRTRFQDGTSVVSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6N1</accession>
    <name>A0A8J2T6N1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial intermediate peptidase</fullName>
            <ecNumber evidence="4">3.4.24.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_06524g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.</text>
            <dbReference type="EC" id="3.4.24.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the peptidase M3 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.59" evidence="4"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6N1"/>
    <dbReference type="OrthoDB" id="17530at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06457">
        <property type="entry name" value="M3A_MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000055">
        <property type="entry name" value="Related to mitochondrial intermediate peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.10">
        <property type="entry name" value="Neurolysin, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033851">
        <property type="entry name" value="M3A_MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024077">
        <property type="entry name" value="Neurolysin/TOP_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF79">
        <property type="entry name" value="MITOCHONDRIAL INTERMEDIATE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 14" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="15">
        <location>
            <begin position="297"/>
            <end position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF88987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="782" mass="89409" checksum="026870211C539A20" modified="2022-05-25" version="1">MLNIHVLRRNVPLFSKWVRLLSTRTLATQISKPTSSDSQVQSLQRTFDDIDYWHEANKKNYELANAKGKFLSNLGLGGSQQPKTGLFKNPYLTNPEGLRKFSRQSLNQAYELLQDMKNDATPDGLRKYILKLDQLSDTLCRVIDLCEFIRSSHPDSEFVEAAQMCHEEMFEFMNVLNTDVDLCDKLKFVLSTPEINSELSPEEVKVGKLLLEDFEKSGIYMNPEIREQFISLSQEISLVGQEFISNTDYVPTNYIKINRQRMDKSGINKLILNQLQQDLKGENYKVPTHGYIAYSILKACPDEEIRRQLWTAMHSCVDKQLVRLNQLVRLRAVLAFLMGKQSYAEYQLEGKMAKSPQQVKDFITSLVKATLPKTANELKFISDLKCKHLKLSPLGTVNGANLEMVRPWDRDYYHALYTMQQRRMPADDQQIGLYFTLGNVMKGLSDLFQKIYGIHLQPVVAETGETWSPEVRRLNVVSENEGIIGVVYCDLFERAGKTSNPSHFTVCCSRQIYPKENDPSTVQTGTNIDGTKFQLPVITLVCNFASSVVSFGQSLCLLHINEIETLFHEMGHAMHSMLGRTRLQNMSGTRCATDFVELPSILMEHFARDKRVLKQIGKHFNTKQPIPDDLLESYLEEARFLQHCETFSQAKMAMLDQKLHDKDIISNLDSLDVVALYQKLEKDLRVLADDQSTWCGRFGHLFGYGATYYSYLFDRAIAAKVWEALFKENPYSRAGGQKFKEFVLKWGGARDPWQCIADVLDQPELSKGGSNAMKFFGESQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6V5</accession>
    <name>A0A8J2T6V5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aminopeptidase</fullName>
            <ecNumber evidence="11">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_10770g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="11"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6V5"/>
    <dbReference type="OrthoDB" id="10031169at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000004">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF174">
        <property type="entry name" value="PUROMYCIN-SENSITIVE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="14">
        <location>
            <begin position="19"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="12">
        <location>
            <begin position="234"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="13">
        <location>
            <begin position="523"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF89167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="861" mass="97392" checksum="5492A881935BCCF5" modified="2022-05-25" version="1">MTSAVVSANREVLPTNVTPLHYDLTLEPDFKTFKFSGHLKLDFKVNDQKLDSIYLNTEAIELNAATIEGQEPLAIENDAENQVTEFKFPQGTLAGKEKVTLDIKYTGELNDHMAGFYRAKYEDKLTGETKYMATTQMEPTDARRAFPCYDEPNRKATFDITLVSEPHLTHLSNMDVKAESVEGGKKVTHFNTTPKMSTYLVAFIVAELKYVENKDFRIPVRCYATPGNEHLGQYAADLTAKTLAFFEKAFGIQYPLPKLDNVAVHEFSAGAMENWGLVTYRVVDVLLDKENSTLGRIQRVAEVVQHELAHQWFGNLVTMDWWEGLWLNEGFATWMSWYSCNEFQPDWKVWEEYVTDTLQNALALDSLRSSHPIEVPVRRADEINQIFDAISYSKGSSVLRMVSKWLGEDTFIKGVSQYLQKFKYGNAQTVDLWDSLSEASGKDVRKVMDIWTKKVGYPVISVKEDGGKITFSQNRYLSTGDLKPEEDETLYPVFLALSTKKGVDNSLVLNERSKTIELEDPSFFKVNAAQAGIYITSYSDERWTKFGQQAEFLPVEDRVGLVADAKALSASGYTSTKNFLNLVSQWNDEQSFVVWEQILNSLSALKAAWIFEPQETQDALNNFTKKLVSSKAHNLGWKFSSTDSFATQRLKVAMFGAACSAKDQEVEKAALEMFAKYTAGDKKAIPALIKPIVFNAAARAGGADNYEKIFHIYKNPVSNDEKLAALRTLGRFEDSKLLERTLSYLTDGTVLNQDIYIPMQGMRSHRQGIEALWSWLQKNWDDISKRLPPGLSMLGSVVVISTSAFTSLEAVDQIKKFFSDKSTKGFDQNLAQSLDTIVSKAKWINRDREVVKKYLKEHGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6X1</accession>
    <name>A0A8J2T6X1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Nitrogen permease regulator 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3 4">Required for meiotic nuclear division protein 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_06920g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Mediates inactivation of the TORC1 complex in response to amino acid starvation. Required for meiotic nuclear division.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Vacuole membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the NPR3 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6X1"/>
    <dbReference type="OrthoDB" id="18648at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056603">
        <property type="entry name" value="HTH_NPRL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005365">
        <property type="entry name" value="Npr3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13153">
        <property type="entry name" value="CGTHBA PROTEIN -14 GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13153:SF5">
        <property type="entry name" value="GATOR COMPLEX PROTEIN NPRL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24064">
        <property type="entry name" value="HTH_NPRL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03666">
        <property type="entry name" value="NPR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0469">Meiosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GATOR1 complex protein NPRL3 C-terminal HTH" evidence="6">
        <location>
            <begin position="858"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="82"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="221"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="322"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="179"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="224"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="239"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="323"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="339"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="352"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="372"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU368069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF90015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="924" mass="104702" checksum="F3D4883169C41A29" modified="2022-05-25" version="1">MNEYLPNPCLLGIQLIITTHSGPQVVYHYPPASVEYISNKQRQSRQNSTGIDDSGSNGKIYAGNLQKKPSIAASLTGQSFISGEHKTPEEDNLKSPSIRSSGTASVRRRRTHSIDSGKVRNVKDSSSSDPDTSSSGLSDSELSTDYVDESSDLSTSSDSERIEEQAAFDVQERRMNELRPSSTNSTNKYANSESGRTVRPKSSQISASKLLEIFNKDGSDRRSSAVSRISTPRTVPSSDNEEDEDRQSELDYSELNEKGKISLDEQYFAENVFQDVNKIFGFDPEFVAEFCSPEREMCNTRYEFTVDTLCFLGLPIHVDQHGNWRKSKRKKQMTRSKRSSSTTTRISKQRSNVSQPEGSNNGTGLARIEGSPTRDHDEDSQSGDDHNSDNQIPELSKEDLEKNMNMFHVCFVMNPLLVEYNQRIDDMFHYAVARLSMLLRYAQAKSNYVSRECHIIMKEKERVLKHSKTYRSIKGAHNRGKYLYQRILVKSSLARALTLCVEKLQKNDIACLEIGDDKVIALQIPIQNEFSVLPNFKLHPVLRGSYLSTILNSRFLSAPPSRDDSLNDYSNAEDEDANDLLHLALLLLDEPANIIKELETFSYQDDIGNIVLTHIVKHIQPNVPLGSYQHLIDDLLGGTSEDTGIGNSFQTSMLRSCALHLLYQRHARVVIPISSRNTYIVSPIAPIRGYSKNDYMGARFSGMPLIYQNQSIFKGKFPTLPSLPSFLQLLSTGRPKPYGNIIPSKEHKSIYLGALSWLLRYGYVTQLLTFVCIRVDRQIKMAVDEDLEREGFKAKTHTVDRDHGVMDNVAKNAKNDVVKTDRRAYSNEEAEDDIAQFEYDDPDIGRDYTIILEPERATAIEKRWMFKCVQDQAPELQKLFSKVLKYFNGRTPMELVVLREGLSRHEIKRLVGALNGYLVEYYHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6Z9</accession>
    <name>A0A8J2T6Z9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ZYBA0S05-08086g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_08086g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAPT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6Z9"/>
    <dbReference type="OrthoDB" id="5376140at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008010">
        <property type="entry name" value="Tatp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317:SF4">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05346">
        <property type="entry name" value="DUF747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="312"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="405"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="501"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF90060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="544" mass="61995" checksum="2F56BE79C426E6C0" modified="2022-05-25" version="1">MDNRDSPHVLDAQQEKTSHNASGRAPCMRSKVIEKMRVMRSHLYREVVHAVEESGGIHDAKDGDLEDIYEVEQLVNMVRIPSSLEKFMIWTLLACSDCFLHYFTITPLRVVRGFILKYRLRERYRSSEMYNDGCMICLIIMAAFFLNKLDTSKAYHRIKRQSSVKLYMLFSVLEMVNKMLASMGQSLFAVLFSRKNSQRALRKRLLMQMLCLVYLILHGYVMAYQTVALNVAVNSYSNALLTLLLSMQFAEIKSCLFKRIDKESLFQITIADVVERFQLMLLLLIISLRNMLAKSKTQPSVIPNSWKLRPTSSLVAGILYGPTINVIGSGLVVDWVKHAYITKFNRIRPKVYDKFLLILCKDHSSSLLRFRDRLGLPIPALAVLFIVMIRPSLVQVLDFSTPNHSALAATILTMGFVALVILKLALHMLLIKWGRFFLDGSRITTAVTENEYVPGLLSGGLGKVDEKSRSIIHLRDSYVGQKSTIVEENIYNGKIVEDSRSSSLVDSPTDVPPGLNDLRRNKNSKNPHSLENVSRYKMVSKQIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7A8</accession>
    <name>A0A8J2T7A8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translation initiation factor RLI1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP-binding cassette sub-family E member RLI1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_11826g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the multifactor complex (MFC) involved in translation initiation. Required for the binding of MFC to the 40S ribosome. Required for the processing and nuclear export of the 60S and 40S ribosomal subunits.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7A8"/>
    <dbReference type="OrthoDB" id="6593433at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03236">
        <property type="entry name" value="ABC_RNaseL_inhibitor_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000144">
        <property type="entry name" value="ATP-binding cassette sub-family E member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000152">
        <property type="entry name" value="ATP-binding cassette, sub-family E, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013283">
        <property type="entry name" value="RLI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034348">
        <property type="entry name" value="RLI_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009945">
        <property type="entry name" value="PRK13409.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19248">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01868">
        <property type="entry name" value="ABCEFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="46"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="70"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="345"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00056499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00067238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00082546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF88506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="607" mass="68258" checksum="C09226C606D2D7DC" modified="2022-05-25" version="1">MSDKNTRIAIVNPDRCKPKKCRQECRRSCPVVKTGKLCIEVTPNSKIAFISEVLCIGCGICVKKCPFGAINIINLPTNLEQHVTHRYSANSFKLHRLPTPRPGQVLGLVGTNGIGKSTALKILAGKQKPNLGRYDDPPEWQEIIRYFRGSELQNYFTKVLEDDIKAIIKPQYVDNIPRAIKGPVQQVGELLKLRMEKEQDEVKYLLRQLDLTHVMKREIANLSGGELQRFAIAMSCVQDADVYMFDEPSSYLDVKQRLNAAQSIRSLLSPTKYVISVEHDLSVLDYLSDFVCILYGVPSVYGVVTLPASVREGINIFLDGHIPAENMRFRTEQLQFRLADTSDDVKDEATREFSYPSMSKTQGDFKLKVDAGEFSDSEILVMMGENGTGKTTLIKLLAGAIPADNGEKVARTNVSMKPQKIAPKFPGTVRQLFFKKIRGQFLSPQFQTDVVKPLKIDDIIDQEVKHLSGGELQRVAIVLSLGIPADIYLIDEPSAYLDSEQRIICSKVIRRFIMHNKKTAFIVEHDFIMATYLADRVIVFDGTPSKSAHAGTPESLVTGCNRFLKNLNVTFRRDPNSFRPRINKLDSQMDKEQKLSGNYFFLDNVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7H1</accession>
    <name>A0A8J2T7H1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_07382g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF8 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7H1"/>
    <dbReference type="OrthoDB" id="2193813at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08049">
        <property type="entry name" value="TAF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037818">
        <property type="entry name" value="TAF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019473">
        <property type="entry name" value="TFIID_su8_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46469">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46469:SF1">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10406">
        <property type="entry name" value="TAF8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor TFIID subunit 8 C-terminal" evidence="9">
        <location>
            <begin position="160"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="388"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="331"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="395"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="409"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="423"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="477"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="490"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="515"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF89567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="539" mass="61591" checksum="FB5D3D13C8683DDA" modified="2022-05-25" version="1">MDTYIQITQLPTLQEFSHDDRELPAEKVLAKAVALQLKPARAHITQFAFDRLLMLVDGQLNDMIAHLHRMSNLQRRESIAKGDLEMLLEGFHLTPSSLDEQQQASGYYRDTYRQEFDHLHSLKEINSNAKDIVSIEDREAVKQNVASILVPPTNPLQKILPKWLPNFPPDHTYRFTPQYSHPITDETVIRRKIVEEGKKSELALAHLSQRTPKGPLQDVEKYDAQLAQEETLAVYGTQKKRKKTTASSADLLSKLPQTNFSVEEYAHGRVEIARKKVLEFEERQWQAQQDPFLKLSRIAFTPFNEKYTRRQAQKEFQSALKRSFLHLVKGLPELERIKSEAEAAARAERNKRLEELRARKIEQEQMGEKDVLDLNEFQHNQEDFFAMESSEDEMEPVRPEHASQKLDESGSNENQTIQQSSEISKDAAKESVDGGQGERVPGSKSAEPRDQANFVQSQESIAKSIDAPVEQLGEQWGKQSNQPSYQQSHKQSDEQQKEQLHEGSRVEPDTGLGPRLDDELNEKAKKKTNDLSGAWSAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7K0</accession>
    <name>A0A8J2T7K0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Autophagy-related protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_08460g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in cytoplasm to vacuole transport (Cvt), pexophagy, mitophagy and nucleophagy. Recruits mitochondria for their selective degradation via autophagy (mitophagy) during starvation. Works as scaffold proteins that recruit ATG proteins to the pre-autophagosome (PAS), the site of vesicle/autophagosome formation. Required for the Cvt vesicles completion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Preautophagosomal structure membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Vacuole membrane</location>
            <topology evidence="1 9">Peripheral membrane protein</topology>
        </subcellularLocation>
        <text evidence="9">During pexophagy, accumulates in the vacuolar membrane region, where the peroxisomes contact the vacuole.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ATG11 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7K0"/>
    <dbReference type="OrthoDB" id="447953at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903599">
        <property type="term" value="P:positive regulation of autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034517">
        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040040">
        <property type="entry name" value="ATG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019460">
        <property type="entry name" value="Atg11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045326">
        <property type="entry name" value="ATG17-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222:SF1">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10377">
        <property type="entry name" value="ATG11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04108">
        <property type="entry name" value="ATG17_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <keyword evidence="5 9" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Autophagy protein ATG17-like" evidence="11">
        <location>
            <begin position="147"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy-related protein 11 C-terminal" evidence="12">
        <location>
            <begin position="939"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="645"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="765"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="944"/>
            <end position="971"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF89612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1106" mass="127123" checksum="D71A43EC1C71C61D" modified="2022-05-25" version="1">MSESARIVNAITGETVATKVSFFTSLEDFKGFVSHRWCIPSDQLLVLLPFGHKLKISTFKECLRNNAVDHYEFYVYDRRLFSAVNNPSGDLTSELQKSKAQDLLSSYVKGSTTGDQSAFLKPINSPLVDTDLKLQDLSYRTITSLLTTNLGWLSALEIDVHYFRSLIQDCISQIIEILQCLSICEQYLNLYCYDVENLYNSNVDFLDQLLQNGLSSNWKPCYENVLGNLEGLEGPLQQYVNEDLLQQNETTLQTLDHQVNTKLKNIKKKLDVNAKFRGLINGEIQTIRSNFTPSGTKDKLDVTMQQRFDELVEETRAKSREILEQDPSEFTETNIEEVKTFLSKIKSEVAAKLFTIAKSLYAQTESLQELKLKLQEKTIVAFGQIAYIQVETLALKKLLLEDCNKDLELYQNYELEFAQIEDLPMIYGLYLIEKYRRESWFLQLLSHCVLLSTDIKQISETEISCRKRWTKNFGNIASIFCRDENDFADMESFDKLFLGGIANLQGSCQEQIQELKKALADTLLIIEVYLERISNLDVPKDVSEVLMQNLSEAKNFRIVSNQTFQKQEYAAPNSNQLNGYKARIKKLETLLHETKISDPGHWPSGILHASHFKPFHNNVSTVSSKITLNNSGNLDPKGNVNVVKVLEMENELKRSKLRLEELQLESKAKEEKLSEAHTKLSDLELERNAFKETMQNLNIELSRLTNQEQEQQKELIKRQAFYQDQLEGIVKENKNLLKELELCKGKYFEQEEVNKKGEQELGKVKDIHNAEKRRLEETSKKLSDDNIMLQKQVERLQQELAELRETQVEKRDEATSPQISEEKDYIALKEFSQTIESEMFEIFTSDVFILENIGLLLLLDDNKQFQIKRVKGLRKAMNQSVLDDSIHVAQSSDISVKSPVYHSIRATHEEIKPLANLERHQLLIEDINKLYAGKLFQIAVIKRFKDIESLAKKLTKENKKKREMLDKIQNEKMTLKNFQVGDLALFLPTRKNNSPMESSASSLNSSFSSVDLSSPPLSEVAPMHTLSKEKSKKNSKQPWAAFTAFDNTTRYFLKDDNEITKGKDWFVGRITSLEQFTADTNGNNSYNLEKNAVWFQISADVISCQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7M9</accession>
    <name>A0A8J2T7M9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">HECT-type E3 ubiquitin transferase TOM1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_02652g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the UPL family. TOM1/PTR1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="4"/>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF89827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000004">
        <property type="entry name" value="E3 ubiquitin-protein ligase HUWE1, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase UPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010309">
        <property type="entry name" value="E3_Ub_ligase_DUF908"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010314">
        <property type="entry name" value="E3_Ub_ligase_DUF913"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025527">
        <property type="entry name" value="HUWE1/Rev1_UBM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF67">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HUWE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06012">
        <property type="entry name" value="DUF908"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06025">
        <property type="entry name" value="DUF913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14377">
        <property type="entry name" value="UBM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="7 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="14">
        <location>
            <begin position="2943"/>
            <end position="3279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1903"/>
            <end position="1926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1967"/>
            <end position="2029"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2050"/>
            <end position="2090"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2288"/>
            <end position="2322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2431"/>
            <end position="2454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1905"/>
            <end position="1921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1973"/>
            <end position="1982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1983"/>
            <end position="2000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="2050"/>
            <end position="2059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="2068"/>
            <end position="2083"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="2288"/>
            <end position="2298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="2299"/>
            <end position="2313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="2434"/>
            <end position="2450"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="12">
        <location>
            <position position="3246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF89827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="3279" mass="376743" checksum="944568F9D723E064" modified="2022-05-25" version="1">MVKLTRYERLQKEQNAQSFKRLMDALTQCTEQEFVEQLKGIREWDRSRDDLFIWIPVLNRIDDMLSNIVKKYSYQTSDAKKHPIKLIEASREDEALCVDLTQFTSRLLCNTENRFIYSSMDVMNSLLNCPNFKVKLGATKVLAIMGERYVISRERFDQDNVLANPQLKRKALRLALSIPSSTMDEEAGDHFSLIELFLDKKKYPSKWSKLKYTYYTSGSSNNRRPFSSSGLNRDVAQSSSMKRLVLSKEDLQSHSLQQLFDKGMEVLPSDDWFDYSIKVTMAKAFSDDSFENIELRKLLIRTKFNAIAVVNTVYVPPQVSSKLFEVDPYAFNTLTEFVSLSETKIPESLRLDSLFALECISLKHVWCSDIVRNLGGNMSHGLLFQILRYISKILRDSSEQVNEEYNVRFFYMISNLADVKALHESLLAAGLIPSLLDIMLVKNTTYRRTLASATHLLEAVINDGDSTSEFIINDGFAVLINSITEEVDFALEHPEYGNPPKYSVVYYSVSFRQLSYIRSLLKLVLKLLKTDSGDRIRNLIDSPILLSLKKILENRPVFGYTLMTYALDIFQRVINSEPTIYSILVEAGLVPYIIDHFFEMMGPCAELLSILPDVISALCLNSEGLKKAKEQNMVRFLYEAVTNPEYAKVLIWKESATDLGASMDELARHYPDLKPQILDCFHDIVKELPRLINFRQPYLYESAHDTQLFYLHKDEEPLEKEEGAGELVFWEVQESTPLVDCFADVIYGMTLDNSTLENLPDKIAIRDLLSIIVMERPPFDYTTSQALLNFTDVLQLFDEQKRDYAFPAMMNVLEERLHRIADFLKSDNEQSYLLRAKKGRDNSDVEHVLSEFSSLTALLYLMTDVYVNITSLSPARIYQIMNYFEASGLGLIESLRLLYQKCALEEMYLTRNMPDKVLTETTPEPLGTASPIQIHATKPVKRTHKDDFTSAKYKNTFEVRFLMNKLQSCTAMLFRCFLRLTHARTMEVESQNIAVEVHIFDEITQQVMKMMHAVRFEDHLPYFLVIFNFNTYIYTFPKTTITGAGILQTTPAYLFYQYGGYKFYSDCIKALFSKMSSFKDIATIEEVDYIKNTDDVLTLSCLLNLMAFYNKSLHLDSMENVGCMMDFHLHPKDDDDYHFTRALMDFVKKSALCLIFELNENGLLFNSQSRVVPYAVFKQVLTMLKNIFTDVTIEEEGVIELSWDLIPPPFKKIEALKACGLSGEDAMSYAKWEDGAEYEKEMKGATDINTLTKKPDVFSDEGWNHYKSLKENGAFDASTIPMKPRYDYHMFSSNRLDTMVDESFEYGLPQKIFDVLPFYPKLVNAIARTLLQIFRNYDEPGEDFASKVLDKVLLTDLDDSATLSSLIHLFGIFLNEKHVYENTGGLIFKFLEYLQQSLSPEYINSAWFSKALYVYEIILAKSEVPIVESIPPEIHLRYRLPPTPPIYRIPPEIKQNIFDCLIRVGEITNFYSALASCRILIFYARDEGYASEIARSGALSKLLRVIGIFQKSEKINFLESSFLLLVRRCFETVDIVSTLIRCELNKSFTTRVIGDHKEKERDLTSLIEEKPHVVMRNPEKFIDNLCETARFQSFSEDGKLQEYNVYRNFQDRPSEKEGADSRKGSTSGIENRTGIIHLLLSQLMAAYKKDWTTEPAQPENEREKHDRPEKVEPTKNLVCAYMMFLLKVLVELVSSYKQCKFEFLTFERRNQYTEYPRPRSTALNFFLYQLLDKSTVHEQNKYEAKRREVISMLARSVIVGFVATVQDDNNKKSDLKTIDPDMNFIRKLTIESIVRALKNTTASSKLLEANVSKLETWFKIISSMVFVQAPYLRLILDSNKLDADQYQICKLMIDMKVPMAVTECLATVDLNYPFAKKLFNNAVDPLNSINSVRNTYADLFKIESNDDEEDVDEESDKEDPPDMFKNSALGMYDVEDIEEDDDEDVSIIGEDDDGIAFVDGEDGDFEVVFSDDNEPHSDHDENSELEGSGDEDMDYDSDEHDLDHMHSGTDGAMEEEEGMNSGDSYYSGDADSDIEVIDVEADDGLEIELGDYDVDESDWESGLSELSASEEDLEDGEDGDNDDDGHVGGIRRRWTTADGFDIMEDTSDEDEAAGVFQGLEHVFHVDAQPLIRVQDARRHNRHHNHHFRRNHGHSLLGAPSLTLLNGGRRHQSNLINPLGPSGLEEVENGISDQLINVGSGVRHRPEHTHFSSVLFSGEFFDERSPDGILLKPTVSRWKDIFDMFYDTKSYASYIIPTVITRIYGPSLRLYHEAEKRRKDEYNDRMEKLRKQETERAEQASRSTSEITEGNTPARSDEEHEHHDPVYVNIDGAEVDIGGTDIDPEFLNALPEDMRAEVFAQHVRERRAEAMRNDVHSREIDTDFLDAIPESLREEILEQETAETRFSNIIHSMRERTHDEDQDEDMISDDEEINDADRTGGSHQRSETDKKKSSRTYFSPLVDRSGIAAIMKSIFVSQPYVQREIYHELFYRLCSSKQSRNEIINMLLMILTEGLTDQNSLERVYNLLTSRASGCTKIQGSNSGGRQLPPDCSPLIVANQAIEILQNLLDSDNRLKYFFITEHENLMVNKTPTKSKQDIFNKSLKWPIKYLFGLLDRKIITDESVLMDLLTRILQVCTRPINVLSKNSATNNSSKKKFQVPYFDRKDFERVVSIINMDSCNTRVFQQTLSIMHNLSMLKEAVQTFTQELVSLALETVKNLVVDLNSLTGKGSLVPNTTEINSELVQKFTMPSSDQAKLLKVLTAVDYLYTHKKNLSDEDVKKLMTIYKEMQLGQIWSSLSDCLFEFESRKVLNTSATVLLPLIESLMVVFKHIKNSQNGGKNPSLKYEEEKKLDFSDAATENFFFKFTEAHKKLLNQMIRSNPKLMSGPFSLLVKNPKVLDFDNKRYYFNAKLRSDAQERPKLGISVRRDQVFLDSYRALFFKSNEDIKKSKLEITFKGESGVDAGGLTREWYQVLSRQMFNPDYALFLPVESDRTTFRPNRTSGINPEHLSFFKFIGMVIGKAICDQCFLDCHFSREVYKNILGRPVSLKDMESLDLDYYKSLIWILENDITDVIEETFSLETDDYGEHKVVELIPNGSQIQVTEENKQEYVKKIVEYKLHLSVKEQMDNFLQGFYALIPIELISIFDEQELELLISGLPDLDVDDWRNNTTYVNYTPNCKQVNYFWRAVRSFDAEERAKLLQFVTGTSKVPLNGFKELTGVSGICKFSIHRDYGPTDRLPSSHTCFNQLNLPSYNSYETLRGSLLLAINEGHEGFGIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T801</accession>
    <name>A0A8J2T801_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ZYBA0S03-04742g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_04742g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T801"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF47">
        <property type="entry name" value="VACUOLAR BASIC AMINO ACID TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="322"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="434"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="474"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="549"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="64"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF88909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="579" mass="63729" checksum="DB9528C22FADE2D4" modified="2022-05-25" version="1">MGDQDSLQEFELDSLDKQSIRLEFPFKQNEISKTGVDNSGGLDNDGEEEDLEIDLRDPKTFHWVLTSLWTNSALSALDGTIVSTTVNEVASRFQQASMVTWVATAYLLTTTAAQPLYGKISDIIGRRKCLLFGEIVFFLGVALCIFSRSIPQLAVARGICGIGGSGTGAMTNIILSDMAPLSERGKYWGYGSVLGGIFQSLGGPLGGVLLTYFGVSGLFIPQIPFCVATLYLSWKYVQDYNEDTKGSWKRIDFGGSFCLLMGISAFIFFFSANENVSLEEQGWSPYQKWSFAIFIISSIGFLIIEKYIAVENIIPMKVLKGSLGLLAVIQGLVAVINYTGLFIVPLFLQLIWNVSVSASGAYIVCIVISYSMGSLLTGTLIKKISKPNRESTMYHSSTAIFYMSLLSAVGYYCLDRSIRLSKPVFGENKLEGNYNLQLIFGITLLGICQGSQGVTIMLFNVAKVGRKGQASATSVNYLFRSLGNVLSVSIALNVFTNALKKNLARVLNDEDNSLYILLLKDNAFLRSKEMPTENLEAILNCFHKSLISSFIPCKTCLIFSVITSAMLWIIIRKQYIDFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T829</accession>
    <name>A0A8J2T829_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ZYBA0S05-03906g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_03906g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
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        <property type="term" value="P:cellular bud site selection"/>
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    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006972">
        <property type="term" value="P:hyperosmotic response"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007232">
        <property type="term" value="P:osmosensory signaling pathway via Sho1 osmosensor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0001402">
        <property type="term" value="P:signal transduction involved in filamentous growth"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="InterPro" id="IPR039295">
        <property type="entry name" value="MSB2"/>
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    <dbReference type="PANTHER" id="PTHR35778">
        <property type="entry name" value="SIGNALING MUCIN HKR1-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR35778:SF1">
        <property type="entry name" value="SIGNALING MUCIN HKR1-RELATED"/>
        <property type="match status" value="1"/>
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    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T836</accession>
    <name>A0A8J2T836_ZYGB2</name>
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        <submittedName>
            <fullName evidence="5">ZYBA0S06-03444g1_1</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="ORF">BN860_03444g</name>
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    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.20:FF:000007">
        <property type="entry name" value="GDP/GTP exchange factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF388">
        <property type="entry name" value="GOLGI-SPECIFIC BREFELDIN A-RESISTANCE GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SEC7" evidence="4">
        <location>
            <begin position="558"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="264"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="293"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF90217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1446" mass="164374" checksum="CA9C01AC3E09940E" modified="2022-05-25" version="1">MDSDCVVAVDPVAIVIKECINLSTAMRKYSKFRSQSGVAALLSNSEIFNNQDDSLADTFNNLSTNKHNDPLLSGFVQLRLMLNNLETLEEIDSLTLLQPFLLTISTSSISGYITSLALDSLQKFLTMNVINESSKNHVAAIREAVNSLTHCRFERSEQLSDDAILLKVVNLLQTVTHSQYGELISDSVMYDVLQTALSMACNKKRSEVLRRAAEATMFSMTAKLFYKLRYIEPVDMTQKYINDEGYANSILKEDIIGMRTSQDDDYVQDDPVVSSEETAENSHLLEGAENTEENAKENASDQKNEEDEKDAKDLTQVTTSQKPDSKATEVVEDNHGLPVIRHYLNLLLSLIVPENQGKHTNSVRIFGLQLINTAIEVSGNTFPRHPRLFSLISDPIFKHVLFIIQNGSKLSLVQAALQLFTTLVIILGDHLQVQIQLTLSRIFEILLNKSNETTSEGRPRPAAIKELLIEQISLLWTRSPSFFTTTFIEFDCNLDRADMSITFISALTKLALPESALTSTESVPPICLEGLISLIDDMHSRLQNVDREKFLAEKNNNDRLKQKERKTEFIKCTKIFNEKPKQGIPLLIEKNFIKSDSERDIAEFLFENNGRMNKKMIGLLLCDPARTSLLKSFIDLFDFEELRVDEAIRILLTKFRLPGESQQIERIIETFSARYVDCQNYDLSKANQNNEEDLAAIQPDADSVFILSYSIIMLNTDLHNPQVKEHMSFEDYCSNLKGCYNQQNFPSWYLDRIYCSIRDKEIVMPEEHHGSDRWFEDAWNNLISATVVMTEIHEQPDKSVTHMSPQDLAHFDGAIFKNVGRSIVDTFFKIFEVASDDHIASRMLGSIDKCSFIASFLKFDGMFNNILCRIATMTTLLDNNNRDAGDNDEIPLVEITVEDSNSKILVNSDACRLGRSFKAQLSMVIFFRIIRINRYSTKFSLELWKKIVTILLVLYEDLMVSPDIFPDLQQRLRLGNLPKPSPEVSLKKARESRGILSAFASYLKGDEEPTEDEIDASTRAMNCVKSSNIPSSIFGNEVNITQELIRTLLSSIESKKTTENSRYFEAEMLFIVELCVGLFLFCKDEKKLGNDVLRKVSEVSMVSGLSKRCIRRLTAYILLLISVVDEQQERLHKLINEEFLKKNEVFTQKYFASEHGSEILRRLLSLTDIDNYQRSILQDEGFWKLLRFMASSKEHTGTIYHYLENSLNNNKNILTNGNFMWILGLLDEISSLGAIGSQWEREYQRLLNSGHKVDRKNPHQDIVDLSLKSIQLTSQIIENKLANNPLSKNETIALIQALAHQCLNPCFQIRSFSLECLEHVLTKIELPSEEIKNLEELIEGGLLPLLDTVTSKEGTNVPVSEILAAISKIYLYRLKQGVTTDETFLEILNIYNRYVEAPEVEKQLQQLITDKKAIEKESNSLDSPANYSVSEAVINRPIKGDTTQTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T878</accession>
    <name>A0A8J2T878_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ZYBA0S07-05314g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_05314g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T878"/>
    <dbReference type="OrthoDB" id="415358at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF100">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="3">
        <location>
            <begin position="209"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="144"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="144"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF90622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="515" mass="58373" checksum="5DC5B0146804A3C8" modified="2022-05-25" version="1">MKRNNVTCSPRQNKKIAELDRKVTSQYQGYIPEAKKTYIDTVQLPKSAQEIQDFFEQYIVARRPCKINGVPSQGSGFPPFPVKELKPKSIAGFLPPDEILTVETKVDGGFGSGAKRVKMTFGQFMDKLMEGKERGLYLTTQYYEDDPNASQDTDEEERDQVLSDGDTDGGDSITFHDLHDDFDELQEANQDEDEQEDELEYEMRLRELYQPPLTNLVETLPERPEFLKCLIPQQINLWIGSSSGAAGEDEEWLKNFDARDPRLGLGRNTPGGGVSSGLHHDHADNLYIPVAGRKRFTLFAPSDAAKMYTVGNIRHVYPSGVIDYVSDDRAPLWRHLRDDGAIVAEVYKFLLEKNEDESGGVLDAEQKTRYEKVIDLDRRKQEQDQIATPAGLDPPSFSTVPPAVMHLDKVRDVKLRESIARASESKWPLFHDANRIVAELKPGDMLYLPTGWFHEVTSFGDNDPDAGGVHVAVNYWFTPPTGNTLNNVYDRQDRYWPRDYEVTKRALECARTASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T883</accession>
    <name>A0A8J2T883_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S06-04500g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_04500g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2123378at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000142">
        <property type="term" value="C:cellular bud neck contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007120">
        <property type="term" value="P:axial cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097271">
        <property type="term" value="P:protein localization to bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13278">
        <property type="entry name" value="PH_Bud4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052007">
        <property type="entry name" value="Bud4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36100">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36100:SF1">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="1461"/>
            <end position="1571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="85"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="195"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="364"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="491"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="557"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="756"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="845"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="929"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1034"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1100"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1148"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1197"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="375"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="498"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="512"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="558"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="572"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="852"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1035"/>
            <end position="1046"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF90263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1592" mass="179430" checksum="D171DB19D832F311" modified="2022-05-25" version="1">MTLNGSMDSLLKEIDDEMEQAAAPDWKLPLHDIGDETMEMLVTHNTMENRHLGIEKHNSRKIIGSKTSSPSKKSVGFSDGVELHEYSTGPEETNQEEDFNTVETRWRRSRPTKLAPLPSTPMMEEPEAESEHEEEPQLTIGELRQHSVTDMGERLSQVLDSKPNMYKLKLMAEEVDNRATQPEEDTRREVFNFTLQPTPPLAPDGSNYNTPSQLTQFEEPQSQQEEEDEKDYETQPENLAQSPSKIPLTNTVSINNFNLDNVNGYLTTRVSSGSSYAESASDLETTTGHDGFKLLEYSSDIPLELQYQSRNTDKYRFGPSKITNHPNASTAYSLATEYESARETPNSSVTDLTSEIRDMSVENLGIPGGLRESSPGFNDSSDSELQQGDDTLREEDLKNKNPALPDLPVEEIVDTEKHISVDSIEGQVQTEIDDQDESRNGIEYAGLHDFSSSYGSLHAKNEDDTVNSKEERFRQSGLYDISSSYGSLHADQEDELADENKESIRQTELHDISSSYESSHAGQEDATPDDSDGVHPQAEWSHLTEDLNASVDAAENLGVSGSGSQESQESFESVKQRKMEKTPPLKARGANIPPLVPSVEPEPIFNEDPFEEIFDTSGDSFDLTRSVKPSDYISIWHNQAGSLKSNSPVFSTNSQFSHRSVSTESSTASIGRNNFKFKSRIISRCKYYNPDSRMIIPSDSEDDDDVIYVVDHAMDPKRRNTLISKQARNNIRQNKKMNPIRAAAVNLHSQEFMKQEEDTPFENYPPTLAPSETLQSVAPPLEGRDSDETPEMDQSQKREKRLVSEIYEFGSYEHLAAIANFDAEHERLVLKNEDSVPCIEEVKEESCSPIIPSSPPKDTPPTTVPPRNEHRNKSLPVLSPPPRNINRKIQTLAPSPTITKNVKVATLPSEIKEQDVLARESFVNEFESPPAPLIKHRSPRRYSSSSSRSQENIAAQIPVSNALDVSPCLPGEKLDEDFGTFLETLSRDDRSVQNSVKTKEGGYNIWNEEFDLTNAATRKSSIPSEVLTKLLETESAVEDPDDDQSENSDSSQRYIKTPTDDVCIGRGLSVNGLEAVVSNGEASERNSVLFDEFHDPSIYKTPNHSPIRRTHVESPFKPISPSKTPKAAKGTEKLEEAHVDMNKALITPRSTESELELEPDSKLEPISQAGTRSTAQEEGEYEISELSSIRHNLKALQPQDGLPDKPLSSGQKMVEQKAPGEISQGKEISEDTEFPDAGNLYFKLKSISGIKLRGVKRHKAQYAIEFDNGKNVAQTPWEPIIDGTLKLNKEFEMILDRNPKHRSKVVITLKCRYEKIRYEPVVEEIVEKVPVISKKKLFGKRKEQYEYQKKYIQKPPKQDDWDFIFARDGSFGRCELVLDEDFLDHARFQCQSISLDLKNEWARQPGAPHKKPHQLPRKPPHFIGQLKIDTCYLARSSALEKFPKTLGIARGIMSKYKEQQNISMEGYLFQNGGDVSENTTRRFFKLKGNQLTGYHEVTRKAKIIINLLKVVEVVGPSDDSSFSELELKKQQNGFQGCFHLVFANGESITFDTEFSTDEKYDWFRKVKRVVDLNKSHQPWVKAFYENSIIRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8G0</accession>
    <name>A0A8J2T8G0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-(apurinic or apyrimidinic site) endonuclease 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_07668g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="11">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF90043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T8G0"/>
    <dbReference type="OrthoDB" id="391817at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020848">
        <property type="entry name" value="AP_endonuclease_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF4">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00728">
        <property type="entry name" value="AP_NUCLEASE_F1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="14">
        <location>
            <begin position="456"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="12">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="12">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA substrate" evidence="12">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF90043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="500" mass="56751" checksum="94DB7E87757E49AE" modified="2022-05-25" version="1">MASEPSAIQKIKEPNSFRFVTFNVNGVRTLFQYQPFSEMNQSPEKIFDYFNADIITFQELKIERLSLTKWGKLENFYSFISIPHSRKGYSGVGCWVRILPREHPFHECLQVVKAEEGITGILGVKSGKEFLQYNKDPSIGIGGYDSLGYVDDKEALKIDSEGRCVMIELACNLVVISVYCPANSTLSEEGELYRVKFLKVLFNRIRNLDALGKRVVLMGDLNVCRDFIDHAESLERDSVVITDIMGGLEIEEKYQKSCRNFLINPEMPHRRMLNQMLSDSIIPAMADEGILVDTTRLTQTRKRLKMYTVWNTLKNLRPANYGSRIDFILVSAKMQDKVKASDILPQVLGSDHCPVYADISVDLSEADPPEFGVKIPKFEARYKYNLTHGNILEMFGKTNRKASVSKVTKKSTLSSSASVGEAIRRTASEHIKQNPLNKSKERMSHFRNCFGEPPVCRHGEEAILKTSKTLTNPGKRFWICKYPRGSLGDKPSSCGFFQWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8I8</accession>
    <name>A0A8J2T8I8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosomal lysine N-methyltransferase 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_12002g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RKM5 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T8I8"/>
    <dbReference type="OrthoDB" id="2529286at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF109">
        <property type="entry name" value="RIBOSOMAL LYSINE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF89220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="327" mass="37277" checksum="0CDF4B3FBC54ED32" modified="2022-05-25" version="1">MPFQLEPLNVDNIHEHIFERYTQLVSLSDEVSQDLGIHQKTNIVHVDIEPPLHARNKREKLNCLESYNFIIEQSLSGLYSSSNGNSTTGYVLWSTTPAFIQWLLYDPNAYELRKGGSVKTIGTSIARSIPAMFSGKQSTGIVELGSGVSGILPVILGNHVDYYICTDQRGILNKMKHNIRENISQLNRRHCLSQSMGITSGEDREEPAGNAVNLEIMLLDWEKFQLKDGTCYPDLYFLAQKSSTVYIVAMDVIYNDFLIDPFLKTLSQIRDYFIQKKLQTHCLIGIHLRAEDMVANFLEKAVLQYRLPVNHVVDSFLESTRFSLYYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8X8</accession>
    <name>A0A8J2T8X8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ZYBA0S06-04038g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_04038g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat L(2)GL family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T8X8"/>
    <dbReference type="OrthoDB" id="19944at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045159">
        <property type="term" value="F:myosin II binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013905">
        <property type="entry name" value="Lgl_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241">
        <property type="entry name" value="LETHAL 2 GIANT LARVAE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241:SF25">
        <property type="entry name" value="TOMOSYN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08596">
        <property type="entry name" value="Lgl_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="512"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Lethal giant larvae (Lgl)-like C-terminal" evidence="5">
        <location>
            <begin position="551"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF90242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1045" mass="116229" checksum="14A64D32879A2B04" modified="2022-05-25" version="1">MFGTKKLKSVSRKFKSDKTKTAKSEGLPPNQLGSSEPEKANTGLLGGRNSRRGFSIRGSDSYGAGTNTNLYTVRELNRYGLKGKAVTAAFDFTQSLLAVATDAGEIHIFGKQQVEVTFTLQAPTVIKHMRFMKGIYLVAIDQKEIIMIFSLYTKKMLQSFFSPGKIHCVETDPTLDWLLLGFTNGVIMVYDVDRDNLSEYKIENLQKTQFFPQERVSAVVSMQWNPRDVGSLLISYEYVTVVYSLIDKQVKQHFIYDLPPYAPGGEYSDTPLKHRKPKVIQSLYHPNSLHIMTLHEDNSMVFWDCNTGQLIQARTIFEADVHIPQNNLVEPIPGVPRIRKAAWICQSNPEYTSLLLLTESKQTGNAFTMIDLGGTPLYSITTYEGMSRYYANTKNQKIFPLEKKIPVVEFLPIPKKSPHFAGCHDPGVILVLLDNGELETMMYPSGLFTYKSTLFPQTLSWIRPRATTAVATAAPKKLWFGMMSVARNNDSILKGGMPVRKDIRASDIRSALATGHTNGSIRIWDASHNELSDSSVFELSLSRILNKGTDLAVEHISFSPETLELSAAIESGEVVFFKFEANQFYNPNAANNTRELEMEFRRFSLNDFKTVLIDVKDRSSEKIKQGFMPETVVHAKKGKVSLVKNSNVGFVCIGYYDGTLIVVDRRGPVVTFIGNVRTIPKISGSCVTAAEFSTMEYGNDGYSSVLLFCGTDGGDLITFKIIPNGKGTFDTHCVDSAKTNRSSIFKIDPFSKETGRSCVATVASMKALTTGACDHGCIIISSADEVQIISPGKSSERHKSFKSPLAATSLCFVPHIDSRGELIVSTVFVSLHMSGQVKVMSIPDLKEILSTNTPLSIPPQFANDCYVLRNGDVYVRLTDCQSILLSIVNLKATGLDKGYNQLTETATDNLYNSSLKIPYRPQVNGLQWARGTVYCTTEEIDTLLGGDKRGLSKYDESRVAGKTITIKPDEPRRTAGDTNEFTYKRPVRNPHRKSQSMFKGVSRAFENKWDEMEDRVNDYAADMSEGLNEVFEDTAKSMTKGALGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8Y8</accession>
    <name>A0A8J2T8Y8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ZYBA0S07-00430g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_00430g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T8Y8"/>
    <dbReference type="OrthoDB" id="2687058at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050794">
        <property type="entry name" value="CPA2_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004771">
        <property type="entry name" value="K/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00932">
        <property type="entry name" value="2a37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32468">
        <property type="entry name" value="CATION/H + ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32468:SF0">
        <property type="entry name" value="K(+)_H(+) ANTIPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="9">
        <location>
            <begin position="34"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF90414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="869" mass="96179" checksum="4413D78DD03CD841" modified="2022-05-25" version="1">MTSSLGGVISGSNPFIYNSGSPLTLFLFQACLILATCNLLHIPFAKIRQPKVISEVIAGIILGPTAFGQIPNYMTTVFPKSSIPGLNLTANLGIILFMFFLGLEVDTGFIKKHLKTALSIGLVTLAVPFGFGCLFSIPLYHSYGKESADAGHTKFTVFMVFIATSISVTAFPVLCRILNELRLIKDRAGIIVLGAGIINDILGWVLLALSVILSSASSDPVNTVYILLCTLGWFLLYTYPLKYLLSWVLRKTHELERPKPSPLGTMCILFVMFISAYFTDIIGVHPIFGAFIAGLIVPRENNYVVKLAERMEDIPNIVLIPIYFAVAGLQADLTLLNQGRDWGYIFASIGIAIGSKIVSGSLLSQVFGLFWRESLAVGILMSCKGIVEIVVLSVGLNAGIIDEKVYGMFILMALVSTFVTTPMTLVAYPDSYRNELQARLMKKELGEEDSLPDNSDAKTEGETAEVGLTSFSDAKNYHISEIVNIINTTETISSSLDLINRLANTGNAMPTDLVSDNIKATSPRSSTSTIKPPISRFKKFTHVWTRNDHADLNTLAVIEENEYGFELAVPVKGIHLRLLTERTADLLQSSSMYNDDLNFTANTDSILQIFDIFCRLSKIQFSSEVIYSTVREKATNISSLRLKENDLVLLSLKGASHETNAGRITRRHFYQNFEDIYLHILGLNELPSSFFQTVASSLSSNFSMLISNSLDRLNADSFMKKSFYLLFPSPNLASSDFLALHLFLVICRTNFRNGDDAQVTIFVNEENIHFTDQLMHVLCDQDWISSALKIVNISIDPKTEQSNTEGFFVDAVLKNGLSDKEYNSLEEATFIMAENYFFGDQPFSDEVKATILRGSNNCFDVLVVHHHCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9J3</accession>
    <name>A0A8J2T9J3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ZYBA0S11-00892g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_00892g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316464">
        <property type="protein sequence ID" value="CDF91353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1043025at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038066">
        <property type="term" value="P:p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051403">
        <property type="term" value="P:stress-activated MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06626">
        <property type="entry name" value="STKc_MEKK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017240">
        <property type="entry name" value="MAPKKK_Ssk2/Ssk22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF32">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037579">
        <property type="entry name" value="MAPKKK_SSK22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1286"/>
            <end position="1574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="204"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1446"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="111"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1446"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="1315"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF91353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1602" mass="182880" checksum="5D5B06CEE2F503F8" modified="2022-05-25" version="1">MSDSDYFNYVGTDPSTEEEHRKAQKKRPTRLRSASTSSRFFSPGKHGHGPVASPPGLGSPTSSLNIGSSPLNTFVLQGRAQQSQSQTILQQQQQQPSQQLSQQPQQPPPQQRQQSQQQQQLQQQQQQQQQQPFSPSEMISPRSSRRPSLSSMVINGKHGGPPPSPNKFLKSEHLIKRHSNSNSDLPVTTPTLVMPPELASLNTQNTSAVPTPQPSMSQVSSQYNVSNSSSTNVTTPATMTNFSQDTISPSSSTKSSMNLANPSSLSLRAFKKQYVLNEQLYLDKMKNSVPDDYYTRGIAPSSINDDEFELEMDDALDNNIKGDDELDFGTNFFTAGTDKENLVAMSSKYLLHRLEWLRQADPTNPNVEKVFKSLDKKTTSEKDFSLTSFANLSMGTQNLLSQLSEHPLVLERFEWQTMLSNVLKGDIVKSEKNKIANQVERAGVSKQYSDDIWLELKAWMNGKSVRDTKKQLEVLRSSSDAVFREIMRFKLKEGASSQEAEEMIRPLINRYYKVTNYWVNLKQMYQSKPVTKTEEFINRVDSMNCWLTFKENFELLTKSLKSWVGNEELDVENIHEGSDELDNSGLFAERIMKEKDIETIFQKKIFFPLAPWILEAKLAFFRYQKHMQELRVSFLSKDLELLLMFPMRLVKEIILIRLAYAKKLKKPTMMMIDQMIEDFTSYIKLSVQLKYTVIVYCQDWPLNVEMDPHFDDTVVEAIHYLFTLLHLKLLDCTKRSFKTFKEADELYKYWEKLKNVGHYIDGAGKVIAKEFNRLTLRLLHRLHSYLLQQQNCPPSFETSSDAERWLIQLFENLGSMKRKLNRFTNFLTKAFQNSVNYKIENHRLLLKKLKETGHFLIYTGGELEQNGIYLIGSAELLGCSDEDIFKILYNSEIGCDMIPKLDIRNSLSLYNAVEKGCDPNTMIVQEVDSHGVSYYHIQNEFSENQARSCIRNRASTSPDMFFDEYQDSEKEMIDLELKLHSLGYVLVLCPDEPMLWEGDMYNLSDGSDIKADDFRNKVSSNTLLLMNQGSSYALEYQCDRFQQVVEDSVLFIERRCSFESVESSLQRINKAYFRFTYSVLSNYSKIINTFRNLSPGIEMLNSVFFFSRDFGRNFLRTNVTTYEKKSVIILLMVKLSVGWLSFLVEDCDPTDQRTFRWCVPAMEFAMQMTSGWNVLGLDEKQFVSLKHNISACMSLLISHFDVMGARAGEAEKFLQQTRPNIDIEDEMDDTSMLQINSVLRMQAIRNLEKNTKRNPRQIGRVLDDTDKGNKYLLSLASSISNVSMRWQKRSFIGGGTFGSVYSAVNLDNGEILAVKEIKIQDAKTMKKIFPSIKEEMTVLEMLNHPNIVQYYGVEVHRDKVNIFMEYCEGGSLASLLEHGRIEDEMVTQVYTLELLEGLAYLHQSGVVHRDIKPENILMDFNGIIKYVDFGAARKIVKNGTNSASLNRTFTDTSASSTEKESPSLPEGPALHDMIGTPMYMAPESITGSSNKGRFGADDVWSIGCVVLEMITGRRPWARLDNEWAIMYHVAAGHTPQLPNKEEVSAAGRKFLRRCLVQNAAKRATAVELLLDPWMIQIRELAFGTGDHENAVESAPSGSEQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TA70</accession>
    <name>A0A8J2TA70_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ZYBA0S09-04302g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_04302g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316462">
        <property type="protein sequence ID" value="CDF91078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="241340at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016423">
        <property type="term" value="F:tRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18091">
        <property type="entry name" value="SpoU-like_TRM3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000022">
        <property type="entry name" value="Trm3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045330">
        <property type="entry name" value="TRM3/TARBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044748">
        <property type="entry name" value="Trm3/TARBP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029:SF11">
        <property type="entry name" value="METHYLTRANSFERASE TARBP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029">
        <property type="entry name" value="RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="3">
        <location>
            <begin position="1294"/>
            <end position="1436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF91078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1444" mass="165587" checksum="5D836BF8D3103E93" modified="2022-05-25" version="1">MLASASGDALIRNYLDDNELIRILSNLIKEDQLAQVLDVLNIISVDLSEHKDIVEQLFKRVTSKLIQQIDYLTSNPYEEYTDGFYNELLANKELRVIGQLMCQLKPLETQLETWIGELLAGYVASDHEKLFERDLYDTFSFTLIANSSGGELKPEINVASAVFLVKFLEVVYSLEEGSPAIAVNGGNIYTIILSLLGCNIETLAFSASFLLRWKIACIADRCMNDEDFDLLTWAIVKAFHRSGCDRVCKDWKLRDGLILILRFLLVSKASPQLMSYIKSDDYWQTIQHALAHDVHEYRKLGISVLRITVDRLTFTSSFFATATFTWDPKDERKNVKLWDQFTTLYEIVALEPALNQIEAATDQILNLFSHPYLHPTWSLILFSTGLKATMESVRKYMISLLFKIEDRSVFSTDLTTLKRTFLPAILEAQYFVVQNSSCPYGERLSTFISAVIAEDKQHPQAVISTALELLVEQPTIFDPSRVYLTFGILNSLKRKHSRLIVPHHLELIERLFDVQSEEEIVETTLQTMNLKFLLHVHPSVSPYMWIRALVAHIKNGKNGYRFINALTDDLRDFAVAFFDYREIEQQFGKYVNIDVTFDLLSLVLLEYPYVRTEALLIIETSKWGVSREQYNNEATKLLSGLLTGERDDEEYNDAHFLVNYPAFGYTTWKSVRMEELYSSLLKQFSKNKFEFFVRVYQKGFENDAYLPDLRWSDVKDLYRIIKLHLVKERDFKIRDEVYGTYMRFLCSFLKVCALDWVKGKDDNELNQLLSLLHENVSNDNGTFLGNLYVSKLCKYILDSYIVISAGVTDEVQWDLVLRLIDILSVIWDDVFSKRLILIQKDLHTSLIEAFFHPTLLFFASEHSSAGDELAEVILKRGQDLLDQSSTRRGYLPLLARNIRSFVVFHSKKCKNSDDGPWWLIYLMLGTFTSTQLSINVFFLRPIIADLFDRKINTYHENGKTLYEQVYGPEEISAKILIIDAFLQCTTHLKEQVFIQTAKRSNLLYAIKETDGAEETQRILQFELLLLGIRSYDKKLLFDFVKDSLLPSLSSERSPLVRIYKEWFTAFCVAEFYEEENASVIEDIVFSSINDHSKPAFTTSNERIAFLVLEGLTNLKNICPQRLLKRFMGAVISSATTNKPLIRHFSNSLMFSFWHKFAQFITNDVLKEIVYQMYLDAKELQLPGQYRVGDANTWDIYQDLTLTGIFGGVLKKTTSASIPFFSEALFKKYLSCEVKWPIGSDDTSMWLNRCTNQAPKASDITFDASSQLQMKSGAWKMLMDIDGVNSEKTVDRSDLVVVASLVDKVPNLGGICRLCDVLGVGLLTVPSISVKKNPQFKNVAVTADRWMPLEELQPKDIAAFMKCKKKEGYTLIGLEQTDKSVQLDDHYKFPQKTLILLGTEAYGIPGPLLSELDLCLEIKQFGVVRSMNIQTATAVIVHSYTVQHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TA76</accession>
    <name>A0A8J2TA76_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="17">ZYBA0S10-01662g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BN860_01662g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SEC23/SEC24 family. SEC24 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316463">
        <property type="protein sequence ID" value="CDF91188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2TA76"/>
    <dbReference type="OrthoDB" id="49016at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01479">
        <property type="entry name" value="Sec24-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000049">
        <property type="entry name" value="Vesicle coat component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000081">
        <property type="entry name" value="Vesicle coat component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041742">
        <property type="entry name" value="Sec24-like_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF39">
        <property type="entry name" value="SECRETORY 24AB, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="13">
        <location>
            <begin position="232"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="14">
        <location>
            <begin position="304"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="16">
        <location>
            <begin position="552"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="15">
        <location>
            <begin position="645"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="12">
        <location>
            <begin position="774"/>
            <end position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CDF91188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="918" mass="101846" checksum="8C95244657B640D2" modified="2022-05-25" version="1">MSHHKKRVYPQAQVQGYTPNGLQQAPIVPDGGMAAYAGAGAPMGYSPQQQPLTAASAPIGGQPQLFTPVQQQLHQQIDQTTAGIANMQLHNVPVVDPNTYYQAPSQAAPAAPIQQPYSQSQYAPASTNSVPMRGGKPMNQLYPIDLLTELPPPIHDLTLPPPPLMVPPEKMLVPSEGSNASPDYIRSTLNAVPKSNSLLKKSKLPFALVIRPYQHLHDTVDPPPLNEDGVVVRCRRCRAYLSPFVSFIDQGRRWRCCFCRLANDVPMQFDQTMSGVPANRYDRNEVKYAVMEYLAPKEYTMRQPPPSTYCFILDVSQNAIKTGLLATATRTIADMLDSIPNHDSRTRISILCIDNTIHYFSIPPDEDSDQVRMMDVADLDEPFFPRPNSMVVSLRACRNNLEKLLNLIPEVFQFNIMSKFALGPALKSAYNLISGIGGKMIVVSSTLPNIGIGKLQKRNESSSVNTSKESSQLLSCQDPFYKSFTIDCSKVQITVDLFLASEDYMDVATLANLGRFTGGQTHFYPGFSATKLTDVTKFTKEFSKHLSMDISLETVMRARGSTGIRSNTFYGHFFNRSSDLCAFSTMPRDQSYVFEMSIDDSIIGDYACVQIGILLSLNTGQRRIRVITVCLPTTDSLSDVYASADQLAITAYHTEKAVEKALSSSLQDARDYANKAVQDVLSVYKKEIVVSNTAGGAPLRLCANLRMFPLLMHSLTQHMAFRPGIVPSDHRAAALNNLESMPLKYLVKNIYATVYSLHDMPDDVGFPDEDGQLVLPEPINASASLFERYGLYLIDNGSELFLWIGGDAVNELIMDVFGTQDIFQVPIGKQELPMVEGSEFNERVRSIISKVREHDDVISYQSLYIVRGASLSEPVNHISAREVATLRLWATSALVEDKVLNSESYREFLQSMKMKISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TA92</accession>
    <name>A0A8J2TA92_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ZYBA0S10-01772g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_01772g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterodimer of SLM1-SLM2. Binds phosphatidylinositol 4,5-bisphosphate, which is required for function. Interacts with the TORC2 subunits AVO2, BIT61 and TOR2. Interacts with the calcineurin catalytic subunits CNA1 and CNA2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316463">
        <property type="protein sequence ID" value="CDF91193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2TA92"/>
    <dbReference type="OrthoDB" id="5598057at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13311">
        <property type="entry name" value="PH_Slm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000328">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate-binding protein SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000078">
        <property type="entry name" value="Slm1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046868">
        <property type="entry name" value="BAR_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046869">
        <property type="entry name" value="SLM1/RGC1-like_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043453">
        <property type="entry name" value="Slm1_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941">
        <property type="entry name" value="CYTOSKELETAL SIGNALING PROTEIN SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941:SF16">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE-BINDING PROTEIN SLM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20400">
        <property type="entry name" value="BAR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20399">
        <property type="entry name" value="PH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="446"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="619"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="619"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00064463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF91193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="673" mass="73998" checksum="FDE7CE681C8DCD3E" modified="2022-05-25" version="1">MSKHNSMTSSITDVLSQQQSNLQQLNNLHIRSNSAEQAISDLPPGGLGAPYGAESAAAAGDPQLRKVHTSYSTASANVSGSTSANVSASCNGNSNGNVNGNGNGTGNGNGYGITATPRRIGLVAPATPATPATPATPAASAASAAPVHPKADPSNPLVVLMPASAQPTEVLANRFSAWRNVIKSIIAYLTETASIQDEIVRQQLRLTHAVQFPFFAVDSQQPTSLEEKAVQSFFLPANAGSIQELPTILSNYHGTLASHASRVSKELTHDVIPRLEDLRRDLLVKIKEIKSLQSDFKNSCFKELQQTKIDMKHYVEAIEEAHHGCPKQDPYLAKIALDRQIRKQLAEENFVHEAFDNLESSGAELENVVVMEIQNSLTIYARLLGEESQLVFDVLLSKLDIGFFSKDPQFEWTNFITNDPNFIAPNLPMRRLKEISYKYQYDALSYEIRSGVLERRSKFLKSYSKGYYVLTPSYLHEFKTADRKKDQIPMMSLSLSECSVTEHSKQGSSDDKFVLHAKQNGLIHRGHNWVFRTDSYEAMMAWFQDLKTLTSITSLKEKIKFVSERLNLSPDGRPKQVQLNNSPSVTLPRGSADLRSLYAHTTGSSNAIIASSGEPLQTSYSECNRNSSASRSDNHSYTTRACSMPDPNVLPINDPSSNLYAGSMQNVSHKWSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAM8</accession>
    <name>A0A8J2TAM8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ZYBA0S11-01376g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_01376g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316464">
        <property type="protein sequence ID" value="CDF91372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="198977at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048211">
        <property type="term" value="P:Golgi vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000552">
        <property type="entry name" value="USO1p protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006955">
        <property type="entry name" value="Uso1_p115_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024095">
        <property type="entry name" value="Vesicle_P115"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006953">
        <property type="entry name" value="Vesicle_Uso1_P115_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013:SF0">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04871">
        <property type="entry name" value="Uso1_p115_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04869">
        <property type="entry name" value="Uso1_p115_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vesicle tethering protein Uso1/P115-like head" evidence="6">
        <location>
            <begin position="363"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="Uso1/p115-like vesicle tethering protein C-terminal" evidence="7">
        <location>
            <begin position="1769"/>
            <end position="1906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1423"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1767"/>
            <end position="1786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1821"/>
            <end position="1864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1884"/>
            <end position="1916"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="691"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1505"/>
            <end position="1717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1435"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1771"/>
            <end position="1785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1821"/>
            <end position="1834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1841"/>
            <end position="1853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1890"/>
            <end position="1916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF91372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1916" mass="219339" checksum="3862F01F2CF72489" modified="2022-05-25" version="1">MELIQGFIQPPKIQSAEEAIPTLCDRVENATLINDRRSAVLGLKAFSREYRESVVASGLKPLINTLKRDFVDENSVKAILETLLILFIRGDGDDDLTRNWISQQSRLQNGKYPSPLVMKQEKQQADQFSLWIADAITQSDELVHLLIELLDTGNFHIRLYTIQLLEAVVSARPTRSRSAIISLPTGVSTLVSLLDDVHEPIRDEAILLLMSVANESSHVQVLVAFENIFERLFCIIEEEGGLRGSIVVNDCLSLIHNILKYNTSNQTLFLESGNLPRLAQILNEPLAGEEFFWNEQRVININTALDIVSLTVDPGNSTAYKNQTMLLESSVLMIVLRLAFHHNIPKNVRPVALLSAADMIRGNSLAQKEFSKIDVPYFDPSLPSPVPQNHLVPIVKLLLNWTFYTNSVHLFSLRVASLELLKSYLHSDGEIQISFLSQEIKDYNGKELTSIKCNLFEAILDYDLELKLNPYKLFFAADLFMYLFESDTSENDGLREMTRKVTAGSGLDEEEQLSSIQALSELIITSLTSEDIRIPISYITLLIFWIYGDQNALNDFLSNRGVIQSLLSFSYQVESDDITVKCLVTMLLGVSYEFSSKNSPFPRRQYFEYITKTLGKDNYFSRISQFKNDSMFAKAPKEIGLNPAFDETGLPRVYFSPYFVAFFTENYYRIKTALLHSCEEEPYAKISYETFEELQNECHMLKQDLKQIESDSSASLLELNEKLNTLKEEHQSLAKYHDDLQQDSAKIKVQLSKTAQQLQETEALLKETTDVKDKLLSEKSENVKTLENVNQINDKNKEKMQSLENKLNSVTAAKNRAEEGINKMNRELMALTRSKGELESKVKELQKKNEKLSNDFKKKEKSLESLIIQKDKYMSSLKLELDKSQEKINILNSQNNALINDSNEWKSKFHSNDKLISKMTEKLQSISENYKQLQTERDGLSSSLQELKLGSNNELSALKNNFESLSASKSQIATENEAFVKELATLQEKYDNFSGKYEYEKSILTSEIKNLNEAVSELEKSLEETKAEKEESTKELGRLQIKCDNCEENLEAEKSAKETLEQRLGDLTQNLNTCRIELDEAIKKNGELDIASHLHLSEAEKLKEELTIKIEALDTTSKHSKELEGELKRKEHSYIESEKNMKLSLTRLEEAKANALTDNESLKIQILHIEKKYQELKAQNAANVEELKKSLLELREERQRDEKKLKELDEQLKEKANLLEGLKEKREKDSELIKNLQEQLGSISCERDELLATKSELVKQLDASKEKLEISVQDVETSRKEYEELKKAEEKTLEEVKELRENSKLEAEGNKREFEKLKQAKMEADAQLQKTEKNSQEKQLEIEALKVAKKKASSELAKLKRNLQETDKEMTNLRIEFKKKVQDFEKERKLLNEGSDSVTRQYSEEITKLEEKLQRTEFELQSKGKELDNLTAAENESRVESEEAMAEKASEITSLKSTIDELKSKSKEATLDLEKQATDSQKKIIELEKTIGTLHMDVAQKNHQIAANDKKLSEYTENCVKLQNELDELNLEFKNQSIKYETLENKESSLQDLLKKANEEKSRCSKKLEERNLQYDELEEKLKTKFSELADLESQRKARQKLEETAQRNERKIVDLESKVTEVDGLRKQLESNLNEMKKLEVEGDFRKSELKMMNTEKNELLGNLEQKSKKVENLEMNLAKKCTELDDLSAEKITIVENLEKLLKERELSLSNLEAEKSSLIASQIKLESRLEESDSKLEKETLANTENEKLRIKVEKLGEELSSLQAKASEYEKETSSLIHEKNSLGVKADSLAKELRNLASSNEQCKIDSDARERQIKEENDYLSSELEKLRLQVAESESSKTKGDNKSPKSSEGNSEVDELMLLVTDLDEKNTKYKAKLKELGVELSSDEDDEEEDDEEGDEEEEDDNDGEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAT0</accession>
    <name>A0A8J2TAT0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S12-01464g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_01464g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316465">
        <property type="protein sequence ID" value="CDF91549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="69964at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010506">
        <property type="entry name" value="DMAP1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056881">
        <property type="entry name" value="Mug62_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22754:SF32">
        <property type="entry name" value="DISCO-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22754">
        <property type="entry name" value="DISCO-INTERACTING PROTEIN 2 DIP2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23024">
        <property type="entry name" value="AMP-dom_DIP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06464">
        <property type="entry name" value="DMAP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24919">
        <property type="entry name" value="Mug62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DMAP1-binding" evidence="2">
        <location>
            <begin position="10"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="1">
        <location>
            <begin position="191"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Meiotically up-regulated gene 62 protein-like alpha-beta" evidence="4">
        <location>
            <begin position="759"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="3">
        <location>
            <begin position="1514"/>
            <end position="1621"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF91549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1642" mass="185803" checksum="835CF0FC17C7C28E" modified="2022-05-25" version="1">MSPSTDLSVPPNLPLHLRNQISELVRDYKEENLTLKGYEAKRKYLLDQYANGQRFPLTPIISPSKRPAALHVRNQSWGSSIRSHGANYYNDVNSITSSIYQGNPTDHSAGSETPYMYQRGRSHRHSLYRVTTVHSNSSYSTPKRRNASSVHLSIPSNEIAGVPYNPMIPLLPREENPSNSDSLTSVLRGRFERYPGEIAMISVNEKGKETSITWDKLYLKAEKVAHILSKEKLYKMDKVLLWYNKSEVIDFTVALLGCFIAGMVAVPVSFETYSLGEITEIIKLTSSKSVLISEECYGQLENLYSTSNHNKIKVIKSEFFAQISFIKTDDLGQYSKAKKIVPTFDIPNISYIEFTRTPLGRLSGVVMKHKTLFNQYNIFANILNSRAMPNWKKHNIKKSFKSRDASAKYTILNSLDATRSTGLVLGVFFNIFSGNLLICMDHRLLQKPGAYENMIAKYKADLLLNEQLQLKQVVINYLEDPQSTISKKHRIDLSCIKCCLTCCTTIDTDVTDMIVQKWLKNLGCIDASTCYSPVLTLPDFGGIFLSVRDQLGKLDNFQIHEPALRYQDELYVNKEKLRGNVVEPSITAMRNSSSSKDYLRVTTFGFPIPDAILCVVNPDDNTLVPDLTVGELLVHSASLTDEFYQMDRVNNFVFKARLNYPKMYSFLNDDPTSTAIGERLSTIKNICPPSLFFLRTKLMGFVHNGKIYVLSMVEDMFLQNQLIRLPNWAHTSDVRKGKKASSQTIDTLSLKSADLKLDKDHKGIKRVVQTYYLQQVTETLVRAVSTVYDVSAFELNHNKDEHFLVVVVESSLSKITTSQENTVLETDSQKRTVEQKMNELTEQVYRILWIFHKIQPVCVMVVPLGTLPKRYCSLEIANSTVEKKFLSGELPATFAKFQFDNVILDFVPHSLFYNESIFSEHLSNLRRNGLLEEYQSNHSTSHLEQRFPLPEENPWQTSGIDYRETSVDLRTQKKLTDFHSVLDILEWRIKETGNEFAFSDGATFGQSPSNENSHKKVSWKAFERIVASFLRKIIESKSPLKRGDRVVVMCENSVEYVAMVIACFYCNLVVIPYPALKENNMEEEIAYFVKVVRGYNVKRIFMDLTTHNLFEDNPVLTKVLKKYKHYIPKITVFSKVKKKNNLTINMFCSQLKQKFGPKPGTNHNSLPCVVWIDDQHDIKRDTHVTMTHSALLKSLKILKETLGLTSENPIFSLCSYTVGIGFMLSCMAGIYVGCTTSLFSLGGVSSNPSGFFIGLQNMNVKDLYLNFDAFCVLMEKASELVAASEGKLSSLEKSSKKHSPAPTLLRPDFLRNVQNLMIPFSGRPNTKKIEELLRRNPRVFVNATKINYVYHNHFNPHISFRSYLGSPPVDLYLDSIALREGLIKTVNPAEIGSRGYIRVQDSGIVAVCTEVSIVNPETNLPCTTGEIGEIWCCSEGNAHDYYICQEGVGPSAKPTLRKDPFITNQFRSKLKTDVDNGLTYLRTGDLGFIRRVFSTDNSGNKVDLNLLFVLGSINETVEILGLTHFVKDLERTVKHTHSTIQKCILAKAGGLLVCLVKCRDNAHAKCANLAALIVSELLKNHGVVLDLCAFAGQSTLPNDWETNRATVMEAWLDQEIQIEAQFGINYGENISIYLLSEFERTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAT4</accession>
    <name>A0A8J2TAT4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S12-00848g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_00848g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316465">
        <property type="protein sequence ID" value="CDF91523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2TAT4"/>
    <dbReference type="OrthoDB" id="447103at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF3">
        <property type="entry name" value="N-TERMINAL KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20168">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="624"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="701"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="624"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="635"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="705"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="739"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="756"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF91523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="767" mass="85426" checksum="EECB4F7A874F0FD5" modified="2022-05-25" version="1">MSFISLFKSLSNFQFPYNIEDKPIHESSLWQVFLGTRKSDSLPVTIFKANQGQQTEPLISNAIHKSKVMRIPGLCRVLETFDSDPQSTFIITERVTPFPWEQLSSLLKAPEGIHVGIIQLLNTLTHLNKFVLGTISPESIFIDSKGQWILFGLELCTERAQVNPSLFGSHMRLYNSLMGLSTVSEDVGKLDGMQLGRLISKILGGSGNVPKDWQMAVQSLSQGHTSIQNFTKKLQTTSTFTTNPLIPLHQQFEELHIKDPPGKLVVMTNLQNAYFDNKDLFHHLFPDFVKGMLIPELTNVITWLISVQSSNPSALSKVIPLLAIFLDLTIEQEHLPESSKQLLYSCFTLQDRQIRFLLLIYLPKIVNHLEKREVSDKIYPRFSMGLADTDSTLRLQTLKSIPVIVPYITERQLNNEMLRYLAKTQVDVDVEIRAWTVIIITKISTLLSSSSGNRANILATAFTKSLKDPNIKTRLAALYGLNETIDLFDVTTIANKILTVIAPGLLDKDPLVRSKAKSLFQRYLNKLEDEAKLLQDETSSNPPTKDIDFDKYCDEGSPNVEDLAEKFLTSILLTSGPASSESTTATAGAMDEDVWANFGEKNASNVSDEFGSFPAKVSSFKTVSSNGAQTSVQETWEDSDADFDDNGWGDFSIDDSEEIKVKSTVGKPAKFEKSWNSDTIDDDWTKGNSRPAVKRSVLASKKKSSILSKPRTSGSILTPHSSSSISRSATPSTLLRSKPTVTKVKSNPNNNITADNEIEDDAWDTAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAV7</accession>
    <name>A0A8J2TAV7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ZYBA0S13-01970g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_01970g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316466">
        <property type="protein sequence ID" value="CDF91717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2TAV7"/>
    <dbReference type="OrthoDB" id="19923at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09394">
        <property type="entry name" value="LIM1_Rga"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09395">
        <property type="entry name" value="LIM2_Rga"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000123">
        <property type="entry name" value="Rho GTPase-activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF121">
        <property type="entry name" value="RHO-TYPE GTPASE-ACTIVATING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="78"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="7">
        <location>
            <begin position="950"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="155"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="774"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="867"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="184"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="218"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="287"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="354"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="414"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="445"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="482"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="541"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="551"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="604"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="635"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="654"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="691"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="774"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="784"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="802"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="882"/>
            <end position="903"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF91717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1164" mass="128815" checksum="1E80425D4930D198" modified="2022-05-25" version="1">MDFGTSLTEFTTASPVPPTYPTCVRCRGDITSGHAYELGDDRWHTHCFSCYRCEKPLSCDSDFLVLGTGTLICFDCSDACRSCGKKIDDLAIILSSSNEAYCSECFKCCKCGEKIKDLKYAKTKRGLFCISCHEKLLAKRKYYEEKKRRLKKDLPELPPELTVPPVIPEKSPRRSHSPSKPFYQPSQSMKSTSGSVISQYLDDIDSKSSNDATLGPKDSNKSSTKEEEDSHREASEKTKDIHEDLNRHGRTVSIDDMLSSTLEGSQEIDKEDEISSHAVNGTPLRNRINNESFSKSPVTHRHGMVVDDSEADSEQHDMISKEQETEKERESSSDAEEKEVEEEEEEEEEEESEESRGETGKEEKEEDEFNARPLESRGLALNLPSHSNPEDTLTLGICDEPASPPTVNAPEVQIASSSPIHKPSTSKLSVPKAAAVSPKLASSLTAKAAPSPSTKAALSPQPKATLSPLTKAVASPKAGPNTTTSTQGASSTSLASGVALAGNSTSSGSGNKLGRSLSMKSKHLVQNLKSRTTGILDPKPLTTSPPTSHKSQFFAFPSSSANASSEATTKTDSDTHSGWGVPRDKSSMPRSPSMHRTTPKGKSDSTIQPEPHSPSSNHVRTSGQDNPDHRRSRSHASVSSSTTTNVSMYRTPPLDNNNSVFGRTTNSSSKAHHRNFSWQTANETVKEEDTSGTQEADTTSNSEISENENFLKKEMAEAELALRKLKLELRELDSKKRQLTSEVDSLRLTKEGLCRDIEGLKNEKERFVMESLDQMQQPDNGVQHQDKIQDKSQEKYQERHQQQLHRHHQHHHQQSQQQFVAQPILPAPKSQEAIVDDISPARHIDTASIARPASKPKFWKIFSGGRLATTPAHTPQHQHEASQSTNGSSGSSSTTNAGSNNNSKLEISNPVLQNPHEFNDVKLMPITNSNSENSSRASPSKSDGTMLYGSNLVARCAYEQNAIPMIVTVCTKHIEGNENFMKTEGLYRKSGSQILIEEIEKAFADCYVTPSPDLLKLMDEDIHAVASVLKRYLRKLPNPVLTFQIYEPLIRLVRESKLLTSVPLKNGGNTEKTQQYVDALEAMVKILNNLPKEHYELLRHLSQHIGKVTSFSECNLMNLYNLSLVFAPGLIRDYNGDRDILDMKERNYIVGFLLGNYRDIFDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WWP6</accession>
    <name>A0A8J2WWP6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">BN860_07316g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_07316g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WWP6"/>
    <dbReference type="OrthoDB" id="4058896at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF88319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="584" mass="67140" checksum="E31F9E718BAD1BD9" modified="2022-05-25" version="1">MTLTLRDLNGSDTGLFNYRHFSKVAILNDTLRRENKIELNSFVDHSFRQKIENLDTATGYYEEENWSEAFCSSKTRYSGRSLNLDSDSVLNFSLASAPKLNNLDCNVHLFTPPVVGDSARLNLEELNAHIALLLEEFARVETGELLVIRLGVFFIPQIVKLNQALARFDNDPKGKATAKCVFKFVELSKRLLKDVWLNDFADFLVIMKSAVQYLEKYRDQYTKIELKGKSDKQLGLIVRQWSMLNYFPAILVQSLNFKVLFAQSSNAPLKEQDEVERNEIIKIMLVTMALEVSKSFIKLINILIGLDECKSLEDCLVMQMMHLLLEYTVESCTGEIMIGVDQAIKAWITEKSKTDPECKSSMHHWVRGILYDYKMRLHTDMSTFTFSSEDEEQVAELMFDKCDLGKIFVEEILRFSQPEKRETPAHGNSIGWLTVFEDEGPALSNTSGEGCNPELQHSTLVSLQPPQDTIVGADPNHIVQAQTTKREAVKKWAHNNKIWLTQEVDKIKEKFHNIFHFHRQPTMITSVRYYNALASRQSHRVPFPGDTTAFLEGQRVQIEKLTRNMYKSEKRGRRKRDALRVIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X498</accession>
    <name>A0A8J2X498_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_00826g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DEAH subfamily. PRP16 sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X498"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034458">
        <property type="term" value="F:3'-5' RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071826">
        <property type="term" value="P:protein-RNA complex organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022613">
        <property type="term" value="P:ribonucleoprotein complex biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000615">
        <property type="entry name" value="pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000018">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase prp16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF91">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE PRP16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="378"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="568"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="338"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1052"/>
            <end position="1087"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF87190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1087" mass="123190" checksum="7C8A03B5982C3677" modified="2022-05-25" version="1">MSDEVVKFVQEHSKSKVTHNFVVTLTKLAQNCRGDLDKFVVGCEALGMLNDDKTYLKTLFIKLSNKNSLDPNLCLSNKVELPRRKLNLQLEYSEEDDDDDESLPIMPQRAPRRPLKFKRIGKDMAQRLREYDKEPKKEKGTSVMRTEPKRINKSVHIARPRGEPLPQMQTYADDEIEQSEGETSDSLISEDALSEDREWYNNDDDYGNAVASQEVSRDLNFRPASEDEKSFRLPTMSVQVYPMSLSKRKQFLPPFLKKNYGSPEFSNSAIIGSFLDASQSGAVSPFKNPDGELSMNAKKGSRLVALRRLQKDKTNRAKETADIVGTALGNLLGLKENEKNRQSASTHVDATDTGMPSRGDIDRARKALPAYQIKSQIIQTIRDHQVTVVIGETGSGKTTQLAQYLNEAGLCKDNRIIGCTQPRRVAAMSVAKRVALEMDVKLGQEVGYSIRFEDCTSSKTRVKFMTDGILLREALMDETLEKYGCIIIDEAHERSLNTDVILGLFKRLLARRRDIKLIITSATINATKFSSFFGGAPLFTIPGRTFPIEIVYSKHPVSDYVESSVMQAIRIHMSTGIDTGDILIFMTGQEDIEATADTLRQKLTEIYSKRLGVSRYEEIDDIEIFPIYSALPADIQNHIFKRLEQGKRKIVIATNIAETSLTIDGIKYVVDCGYSKLKLYNPKIGLDSLTITPISKANADQRSGRAGRTGPGMAYRMYTEEAAYDDMYNQTIPEIQRTNLSNTVLLLKSLGVDDILRFPFIDPPPLQTLLASLYELHFLGALDNWGNLTRMGSKMSKFPIRPSLSKALLISATNGCSEEMVTIVSMLSVPMVFYRPSERQKEADRARSRFFVPESDHLTLLNVYGQWRSNRYSAKWCSRHFLQYRSLQRAREIREQLIKIMQSQEIELVSSGTEWDIVRKCICSGFSYQAAKISGLGKYNHLQTGMEVQLHPTSALYGMGDLPPYVVYHELLMTTREYINCVTSVDPFWLMEFGGFLYEIKRIKDMEGNGGRGFMDTVSDDQDETENQPDELDLRVKNCLVRRDMALQRIQQCDKQSTKRYSDGPNSSQKKRENSNVQIGFTKRRPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5F0</accession>
    <name>A0A8J2X5F0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">mitochondrial processing peptidase</fullName>
            <ecNumber evidence="5">3.4.24.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Beta-MPP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN860_12926g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalytic subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins. Preferentially, cleaves after an arginine at position P2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.</text>
            <dbReference type="EC" id="3.4.24.64"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.64" evidence="5"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5F0"/>
    <dbReference type="OrthoDB" id="10251424at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000002">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000001">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF149">
        <property type="entry name" value="GH01077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="16">
        <location>
            <begin position="42"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="17">
        <location>
            <begin position="194"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CDF87721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="467" mass="51444" checksum="0E0A55871587E21A" modified="2022-05-25" version="1">MFRNSMRMARKTSQIKRRSLATGVAQSLPVTRTSVLPNGLTVASERLPNVSSATVGIFVDAGSRAENAKNNGTAHFLEHLAFKGTQNRTQTGIELEIENIGSHLNAYTSRENTVYFAKSPEPDIPRAVDILSDILTRSVLDPKAIERERDVIIRESEEVDKMYDEVVFDHLHDIAFKNQALGRTILGPIKNIKTITRDDLKSYIDKNYKGDRMVLAGAGAVDHEKLVKYAQRYFGHIPKADYPIPLGSPRGPLPVFHRGEKFIRDNSLPTTHIALAVEGVSWSAPDYFIALAAQAIVGNWDRALGAGTNSPSPLAVEASDNGSLANSYMSFSTSYADSGLWGMYIVTDSQEHNVKDIFNAIIKEWRRIMSGNISEGEVERAKAQLKAALLLSLDGSTAIVEDMGRQIVTTGKRLSPEEVFEKVDKIRKEDIVAWANYRLKDKPMSLVALGNTATVPGVSYVEKGLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5G4</accession>
    <name>A0A8J2X5G4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BN860_15324g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_15324g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5G4"/>
    <dbReference type="OrthoDB" id="1577640at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08578">
        <property type="entry name" value="GDPD_NUC-2_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14483">
        <property type="entry name" value="SPX_PHO81_NUC-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057506">
        <property type="entry name" value="C2_GPCPD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25329">
        <property type="entry name" value="C2_GDE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SPX" evidence="5">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="391"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="499"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="534"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="6">
        <location>
            <begin position="781"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="581"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF87825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1089" mass="123385" checksum="0FFBC59366F9BCC9" modified="2022-05-25" version="1">MKFGKYLEARQLELPEYSSHFIDYKGLKKLIKHLAVPLAQPQSENQLTLDEVDEAVIFQRLQEHKASFFFKLERELEKVNFFYLEKESNLKLKFDILQSKFKHYKSRGKLTSKEAVSYKNIHGGLKKFQRDLANLEFYIELNRTGFSKLLKKWDKRSHSHQREFYLVTVVSVQPVFTHNEVSRLNDATLSVLMKLDDITYEDGTSFYSEVSPPPSRMNLIEAPHLPAGRPSLDAGATRLVDIDAEIENWYMEIVNISKLKDTQRKMDMLKEFHVSKIEKFLDEAIPSSRIDKNLIFKACFTKLFLLLVSSSMDDVSLHVFYSRAKAHIDLSYCDEDDQVFSRRNVFHEAANCVKHSRIFVFREAFLITSQTVPWALTADTLRRLLNAKDLHQRTPLHYAAELGKLDVVELLVDSNLLDSVDILDDDSRTPLVLSIIANHIDVVKKLLVDGHASPSPEISEYTKPQFAPLIVACYHNNYAAAKLLLDIGQINLANITDPGGLGVLHIVAKKGGDAKLIELLVLHGADPNGIDGFNKWTPIFYAAQEGHADTVRELLNHGARMDVTDENNFGITYYAIWEGHISVLNVLLQRASEEYKSKEKRIAVLQSNTPYTRPMSPSSSLQDIPDFALPPPIIPLRKYGHNFLEKKIFVKLIIKSGSNSIDFIKGEEVILPSPGRITLTSNLPDIIPRNIILPLVEEEDGEVVFQVDSLENFSIDFEIFPVFGTRIIAKTTAMTSVFRNRLANGAARISLPLFDTRLMNVGTLTFDYQIIFPYNGRPLEITKYDTYWKSSGGAGTDADISREGHRVVTSSSLSGSFVSVAVCTLNDGTIIAAPDMYLTVKDAKLFFNDLTRDQVEKMCGYRFDEFPPIEDFENLKRILSSRIMLFSEFLNKLPPSIQLEIQVCFPANLEIEDIPVKISPFIHLDGVIDSILLLVFEHVRNLRHSGQIMRSIVFSSCNWQACSILNWKQPNFPVLYHMKGLKRENGLFIRDTPHNLLDLAVNPSSINFSDERSKGIHQIVSFAANNNLLGVIVPYHLLKICRLLIGAIQGHGLLLIGSAEQAQPEAFEDEDINGVHTTHELVFSKSIDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5L7</accession>
    <name>A0A8J2X5L7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BN860_16490g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_16490g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the membrane-bound acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5L7"/>
    <dbReference type="OrthoDB" id="420606at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051085">
        <property type="entry name" value="MB_O-acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285:SF18">
        <property type="entry name" value="PROTEIN-CYSTEINE N-PALMITOYLTRANSFERASE RASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="412"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="479"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="511"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="544"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="584"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF87876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="618" mass="70363" checksum="69C491D685BA4D9B" modified="2022-05-25" version="1">MQALLDGLGNFFSFGVLDYRLEPSPGVRRRQMLLSKIYATSSGRAASKNAKKEGLSVSAAPPPLWNTWEFRFYYLAFAIVVPLMIKAVMDASNEKNPNYPLYEPLLSPGWIFGRKADNSDLQYSFFRDNLPLLVGLMILHTTLKRIVLKLTSISKVNFDFGFGIIFVFAAHGVNSIKIFTQVLLSFGIGRAFKANLVTATLLTWIFGISLLFFNDKYAGTPFGDLMPCLSFLDGKFEGLIPRWDVFFNFTLLKMISFNMDFLEKWAQRADKVENSQSHSKLTPPGSRGDSPGSVLFKSGSTSAASSTLELSPLDLTLNEKARLVAPHPLNDYSLVHYIAYVTYTPLFIAGPIITFNDYLYQCRHTLPSISIRSTVVYGIRFAFCLLSMEIIIHFAYVVAVSKTKAWKGDSPFQISMIALFNLNIVWLKLLIPWRLFRLWALMDSIDPPENMIRCVYNNYSALAFWRAWHRSFNKWVVRYIYVPLGGSSNRILTSLAVFSFVAIWHDIQLRLLIWGWLVVLFLIPEMLATKYLTRYADRKWYRPLCAAGGVINIWMMMIANLFGFCMGSDGTKQFLHDTFGTWPGIKFAFVASACLFIAVQVMFEQRQNEKRNGIDLRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5M4</accession>
    <name>A0A8J2X5M4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Heat shock transcription factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Heat shock factor protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_16864g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">DNA-binding transcription factor that specifically binds heat shock promoter elements (HSE) and activates transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homotrimer. Homotrimerization increases the affinity of HSF1 to DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5M4"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032993">
        <property type="term" value="C:protein-DNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000027">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF427">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="13">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="312"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="379"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00059868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00084017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF87893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="538" mass="60591" checksum="EA5F139793ADDE09" modified="2022-05-25" version="1">MDRSPDKEVAVPPLNDDDIEQILHPNNLFTDELRQLDRDSNSGAIEDIINPSVDQSHGTRPDNAMQRLTPMRVNNQLLPSRMFRPQPPPQQAPRPSTHRTRPAFVNKVWSMLNDESNSNLIKWAEDGKSFVVVNREEFVHQVLPKYFKHSNFASFVRQLNMYGWHKVQDVRSGSIQNSSDDKWQFENEFFIKDREDLLQHIVRQKSANQARIHGLNENNDMHLSEYHLNDSGNLTALLNELEQIKYSQMALSKDLIRINKDNELLWKENMLARERHRTQQQALEKILRFLASLVPHMDQKMIAEGIFNDGANGESSAQGVQTPSAPPTGVPVGGSGTSVPSPGGANGFPYDNDMADLQNLYEGALPFEQRYLLKNRSPSLHYDSVDGSTGRISEIPYDEDDDPKSNRSSFLSHLQSNMEEQDARIQHLEDMVSSQANPPPRTGFTPLLQPDDNLMSLQENSPGVVVQDLPAHNVDNVENVGNASNVSNISNASSINNVNGANNVKSLDLLNGVHNKRSLVESDDDPLIEEIDTKKPKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X611</accession>
    <name>A0A8J2X611_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">BN860_03378g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_03378g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01377">
        <property type="entry name" value="MYSc_class_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000101">
        <property type="entry name" value="Slow myosin heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF857">
        <property type="entry name" value="MYOSIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 8" id="KW-0518">Myosin</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="11">
        <location>
            <begin position="93"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="8">
        <location>
            <begin position="643"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="834"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1048"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1233"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1424"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1536"/>
            <end position="1577"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1665"/>
            <end position="1790"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1874"/>
            <end position="1901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="11"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="186"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF88146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1922" mass="221985" checksum="4CD279101E46E84A" modified="2022-05-25" version="1">MDSMTRALFEQHSSLPTRRQPNSSGSQGSMVEQHSLVWVPDGEKVFVQGDLIEEKVVTDKGNKEKPLSVVRVQGHEKEFAQSEVCPVNPSTFDKVDDMSELTHLNEPSVLYNLENRYHDDLIYTYSGLFLVAINPYCNVRIYTQEYVNLYHGSPKEDNKPHIFAVAEEAYQKLLCDKQDQSVLVTGESGAGKTENTKRILQYLASITSEDKLLSTKQESFERKILESNPILESFGNAQTVRNNNSSRFGKFIKIEFDERGKINGAHIEWYLLEKSRIAYQNPLERNYHIFYQLLQGLSSQELRKLELNSTSIKDYRYLCNSNASIPGVDDAQDFQELLSAFKIMGFADDEVNQIFQVISIILHLGNVEFVSEKAEQASIKGDISALCRLLGTAENDFKAAILKPKSKAGREWVAQAKNAQQSRFILNSLSRTLYEKLFSFIVDKINRSLDHGSMTANYIGLLDIAGFEIFQHNSFEQLCINYTNEKLQQFFNHYMFVLEQNEYLKENIQWDYIDYGKDLQSTINLIEQKGPPTGLLSLLDEESILPKSTDDSFFSKLISTWDQKSNKFKRAKKPSCFILKHYAGDVEYNTNGWLSKNKDPLNDHLLRLLAESPNELIKGFFSESPKVRGGSFRTASSRHREQLSSLLDQLSSTNPHFARCIIPNNKKKAKDFDKRLILDQLRCNGVLEGIRIAREGYPNRIFFKEFYQRYQILADDTKVSHGAKKNSEFLLSSLHLDPSLYKVGATKLFFKAGVLAELELKKEEKIFETAIKLNAYVRGGQIRHQTNQQLQKLRAARVLGETFRAHNKMMEDPWYNLYLRIKPLLSSSQDIAKSKKVAKQVKELEAKLKTAEGERDKALENESSAARDLDELRRLLATEVEKLKDHQELLATAKENQSSLQQRLEDANKLREQIEQERIKKEEQHEKALQELTQIKQTSNDGEEQLKDLEDKNAELKKQVERLQAQIAEKESALRSLEQSKSKISKELVTIKSKESTAEEELAKLKSQQLTTKREMETLRSAESARKREFEKLKMNETSKEKEIATLKSKLNSSEQDVNIKLNNLEKNCEAAMKRLKTLVTENTDLNSKIEILEKEKNSYTRELSSKERELNRMKEKTDQHQEEMSVLASQRDNVVGEHNQVVEELKETRAQLMDYKMKLQHLEKEYDTLSAQSSEDKKVNGSLRYDDSKVKELERRIAQETSLNHYLTQKLSSRPNSPRPLSEMGPMGEEELRNFEEINIQLEESKRNLEAQIEEKKDLISRLRFTETRLASSSFDSQLAHTQVKKLKEIIKNSNLSIDLDKELQDLKPSDINLEKLVLEIDHLKRQLDIETKARYDAENVVSALHNKFSQIQRSDSSSDIYRLKYEASEERAKSLENKLRHTPLKDRTNLAGSIFTNRENFSKYADEVRFHKLENYKLQEVLADSNKKISKLSHEIKQSSAKEVMLSEQIERLEKDLHSTEGQNQMLASTIRQQKAQFENCVNDLHETESKSREYINALKQAEEDVQSMVAVIDKMKTHIKQKDKVIWQRETEKNELEMQLQETALELKRAQDINAMLDADLTHLKERLAKREENSQSTALIDGLKEELDTLMKSETGMKKEISTYKYQLETLANDSQAKIGELLKQVQHYETLVATLSDERDMADAAEKDLTKKLEIMMLRADGLAETVESVTKEKNQLDTEIENIKNELLKSNSNFEKTLQENHDIANNVSYLKETLQLQQDQNARNEALVKQLQEEVDLLKASGKEEKQRFIELNEEYQALERLNSQAKTKVNFLESQLADTSEKDAWLSKMHQLESLVTKETQEKYEEMKKVKNLELVVESLKEKNDNQAEIISIANDDKEQFDVRVLQYNEQIAVMEKHLSKQDMDLRKCVRDNANFQNKVEGLEREISFWKERYDTLASGRSNAKAAHSEEVFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6H0</accession>
    <name>A0A8J2X6H0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">BN860_16886g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_16886g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6H0"/>
    <dbReference type="OrthoDB" id="4034100at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF87894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="335" mass="37468" checksum="A27575D38459F693" modified="2022-05-25" version="1">MGVPIDLYKVSGRRRYLDCPEWLKLKLEGDMQKANVLQNNMNTFRFHPLGWKLVLNNCVPMGDSQLSACRGMYWVSIAKGRLTVWEGGIVREVDLPFDDIPSCVDCRRTGEALYVALGMQDGVHIVRFAPDGEETWHSVTRGKCLALDVAMAPRYTVCLIEKEGVLVAGRGGVFRLSEASAAGTEPVDVMVPQVAMGSRHGGVFDSCLEDFDHMLSLEERVLSTKCIPCGVLLQTNIRLVSVQNGELRTISYGSPCFCYDGAYLYAAEQHLYGTCFTVYEYTEREWCNVGYYDIRANFGIRKVMGLNVSDTRVELLGEGNELYVFDIQPASYEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6I3</accession>
    <name>A0A8J2X6I3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_17612g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6I3"/>
    <dbReference type="OrthoDB" id="762982at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF21">
        <property type="entry name" value="CHROMATIN STRUCTURE-REMODELING COMPLEX PROTEIN RSC3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="16"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF87926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="810" mass="93151" checksum="3E250B377CC30C31" modified="2022-05-25" version="1">MNVMDIRGRKMKKPPACVQCRKRKIGCDRVKPVCSNCQKNGKSDCFYPDVPGQYVVSNSASQNSNGGRDGAVVDTPQNPELASMEQIREYNTRLQLLNAQHQQQRASPGPAEPTQFIPRTVQTFENKPVSSANGSAMHLNWVQGPAIFDIMTSPYTQEEVLWKEMDFLRSRLLELQEITGKKVDVDLKTESRKDGESELSETKRFKPSMDGYKTVNEFRDLDPQFLDSKSVFSIFDTRDSDVLAALNPLMDTPNCVFNVPFLVVRDEFISRFYEKFNSVTKTHFNDLVSQWRRLRVAGVPLYPKGDTQLRFPPRSLTQEIITKYIATVADNNSLVPILKPKDLLQIVEQFFGRDSIFAPGKLELPQLVSLGQVALCLLITYETLASSVLIPLREEQLTMFNQLKDWISTLRADLHLVRSEIDRRKDSARSVDVLRFVALWKYYETISDSCDNNSYDGDEDVHLARQLAINHESQNQLLILLWNFIFKNYCWRHLFKGEIPALASSSELNSMTVIDPLLNNDLSLLNFQIDMVKYLHTRDSVMSLIKVLRLKDLFKVKLHEQNKKCYTTAAIINSVVDSLIYHNAMLFLNYYLLMQYEALKDTERFTDGYKEFLQLVQETLFYVFSNLANLKFAGYEFMFANKSFITLNNICHMILGLYERCHQSIQNFTGTDDNLKEEINQQAETLVLLLRKILMLLQDYSKNRKVASAHIEKLVCKIRTILEYTAYCSRPKNTTLNHLAPSHFTSKGTNAFENMDSSALLKIVTKLRGISESLIKSDFYGQRKPFETAYPDTLGITKENFQTIFDSFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6S8</accession>
    <name>A0A8J2X6S8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BN860_14070g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_14070g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051625">
        <property type="entry name" value="Signaling_Regulatory_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872">
        <property type="entry name" value="BTK-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872:SF2">
        <property type="entry name" value="INHIBITOR OF BRUTON TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="137"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="198"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="251"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="762"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="992"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1063"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1105"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1174"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1304"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1004"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1034"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1081"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1108"/>
            <end position="1138"/>
        </location>
    </feature>
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        <location>
            <begin position="1174"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1310"/>
            <end position="1323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF88601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1333" mass="151350" checksum="C058D8FA722756DD" modified="2022-05-25" version="1">MTRQSQDALKRDVFGRNLSYLKSCLPQPTNIESIDLYSHELESGYTPLHATLKLGYLRDSFRLYKHWKDEMEYLSHKFGGHIMNQTDREGLTPLELYDMELQGPTARYPNALGYRINDDDSLVKWGSYSQESNSQGPYVLTWGSNVNYQLGTGTKDDRQNMFQLAITQLETNGYLPSSENFKQIFIRRYHSLLLTTNNNIYVCGTGSRGRLGNGTSESDEPTFTQVLDLEDVDVRMISTSDHHSLVLTSDHSVYSWGWNAYGQLGHTSNRRIEEKNINKVAISAAKRIGFLDGEDISFVACSKLHSCAATRSGHVLMWGLNLGQMGGSKPVHSTSDVQYHNEDGYIVRTPMIVKLSNCEIEQIVCTELATFIRTKGNSLHILSNYSIRTFKIPLPRSRAFKEVDTFDHFTPREVPSEVVDMKCSNRFGNKICFRYSCGRVGIISIKDETVRMWSKMSNSLPVSLYWTPNLKGRKCLDFDVSAQGKIIICTIGGEVFTIDGVNSRPEKIYSSKLVTGRALSVSCDSSFGSFAIIKKEINGIPMIYPKDSLYFDFSQYSPLKGKRRSRKGMIWGIMNEPKMQHTDYLSSQKLANDLETEDKPLEVERIKSIGSSLADFEHSKITELSQEAVLDEANFDVVFISGYTKTPICYCHKLILKNRCTSFIEKLTKSGHYESKNCFHKFTFECGIADKCWRINIWAEKDPTETLLQLTHFLYTDERDFGQNTSRLLFELVDNSHHLAKLSVTLQELVRKRNNENCVDGSDVAIIVADGVLKAHSLILSTRSAFFQAILSKDWVSADKNGIKTINLEHIEFANMQNMEFILNYFYGMEYEEICSSLDMGCFGEYSQFFLETLELTDIFSLEGFKNFLESVVAKYITGETVIPILINATYSNCRLLSFTCCSYLCTHVGILFSRENIELVNQYFDHGLWCLLEEVLIDMRNHSVKQQCKPWYANVSFDWISLFRNNLKAFNERFMDSKHFFIPTFDLKPPVTEQKSRTKSSNRRASITHTQNKNRQSSSSSFTGEKSFTRRPSHLEEKKTVCQDRTDDTAIDDTDDFVEVIKKPKRRTSSQVAKLLPDQKPGDHFEGSDHRDCSSKVVIHSNADDCSSSLPSLLPSSGNTDSGSVMESESSSAKMSGTFKKVSQKQRKKIVVTDDKTFDEHVWKKTSWGSRISTTHSHNSVASSKPSLPSLYDRNSSSTLNLSKKEKKKSKQLAPNTEFVSHGNMGGISPYLQKTAIAQASLAKAPLASPKLTLEERVAAQEFEKWFAEQSENVQKQLSKKKNKSLARELDVVYNAAQSLPDFLAHSREKPKKHKKRLKLKFQSKQAQDSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6W3</accession>
    <name>A0A8J2X6W3_ZYGB2</name>
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        <submittedName>
            <fullName evidence="3">BN860_15104g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_15104g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6W3"/>
    <dbReference type="OrthoDB" id="3993315at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="40"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="306"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="381"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="467"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="566"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="129"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="185"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="199"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="215"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
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        <location>
            <begin position="255"/>
            <end position="268"/>
        </location>
    </feature>
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        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="329"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="405"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="473"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF88646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="653" mass="72729" checksum="271D9F99ABFF9154" modified="2022-05-25" version="1">MGLDKETIEKRVSRIELDIDHMNQMIDENLKLNEAGDTADKATEAIGDEKVEGSELSLKAEGAEPRPDRSSVIVNAEENMAHQGGSMNDVREEVKTEKNFSETVRKAADSFVHGANRKEEMQANEKTSDSNSPLKSIKQGSETSEDASLTRETSLGRFEGGEMAYDAQESPYAGQEVQLTRSEISNDEYYDWSEEQQEQQDGLTSESCPTDQDDPEKTFQADEVLQHGQEDNVGNGQLHQAQLPQTRASSVYGESNDEWEDEPEEFEIEPVSHSGLEDATPNATPHIAQADIMPEQDTPIKDNTYQFSEAQYESQKPQTANTNNLSEIPYEKETQQQKELKAGSDASGNESENETVEFAYKAQPQVGMQYDMQLEKTSNSAQTGIFGSGGSPPEAERIGAGHGAHTQNSNTSNGGFSEHFYDVYDEAQQVDEEPDARSSVAPHPIPSQQRENLYSYSYETFEMVESMPPRTADTSQDSHTSLSSDSIYGESLPNTADTHDSIYIPKNNAKIQRPATTNNTPVASRRPTNPFRVVSVGVVGADGRTSRKTSAGTYSYYKDNHQIDGFAMLQRKLDHLTKKCAKLQKEIDYLSQMNSSSSLPIDDSRKLSRAIAKLQEYFDKKNKEKYEVGVLLSRQLRKEIDRGENGQFWIGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X785</accession>
    <name>A0A8J2X785_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="16">ZYBA0S03-04830g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_04830g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X785"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17997">
        <property type="entry name" value="DEXHc_SMARCA1_SMARCA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000082">
        <property type="entry name" value="ISWI chromatin remodeling complex ATPase ISW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000002">
        <property type="entry name" value="ISWI chromatin-remodeling complex ATPase CHR11 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000234">
        <property type="entry name" value="ISWI chromatin-remodeling complex ATPase ISW2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ISWI, HAND domain"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="SANT/Myb"/>
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        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
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        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A MEMBER 5"/>
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    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
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    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
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    <sequence length="1083" mass="125978" checksum="B47919BD984D612D" modified="2022-05-25" version="1">MTVDQKYWDELKPFQEGMPAHDPDSNKERYLLKDTTSKDKFDIQGTTQRFEHLLSLSGLFRHFIERRAQRDDRFKKVLEVLDSNNGKKSSKGSANSNKRRRKTEREEDAELLKGEEAEEADEDGIDFQFTESPPFINGTLRSYQIQGLNWLISLHKNGLAGILADEMGLGKTLQTISFLGYLRYIEKVPGPFFIIAPKSTLNNWLREINNWTPEFNAFVMQGTKEERSELVHKKLLTCDFDIVVASYEITIKEKTYFKKINWQYIVIDEAHRIKNEESMLSQVLREFTSRNRLLITGTPLQNNLHELWALLNFLLPDIFSSSEDFDEWFSSETTEEDQEKIVKQLHTVLQPFLLRRIKNEVEKSLLPKKELNVYVGMSSMQKKWYKQILEKDLDAVNASNGQKESKTRLLNIVMQLRKCCNHPYLFDGAEPGPPYTTDEHLVYNSAKLKILDQLLKKMKEQGSRVLIFSQMSRLLDILEDYCFFRNYEYCRIDGSTDHEDRIRAIDEYNSPDSSKFLFLLTTRAGGLGINLTTADVVVLYDSDWNPQADLQAMDRAHRIGQKKQVKVFRFVTDNSVEEKILERATQKLRLDQLVIQQSRTSFQKNKKDKTNDSKDALLSMIQHGAAGVFQSSPTPSRANTAQPEGEDHNGDEMDLDVILANSENKTKSLNEKYSALGLDDLQRFNQDSAYEWNGQDFKKKVQKDIISPAWMEPTKRGRKAADYSIDGYYKDVLQTGKPATPSNPRMPRAHPYYSHQLQHPQLKIIYEKERFWNAKKAGYTPNMDDVKTTYGDITDEKEKEQKLEILKLSVNNAQPLTEEEEKSKAEWEGEGYTSWSKTEFRKFITASGKYGRNSIQAIALELAPGKTADEVRDYAKSFWSNIERVEDYEKHLKFIETEEEKIKKIKHQQEALRRKMAQCTNPLFDLTLKHPPSTNNKRTFSEEEDRFIMIMLFKYGLDRDDVYELMRDEIRDCPLFEFDYYFQSRTPVELARRAYTLLQCLEKEFNNGIPLDEQTKKRLHDEDEAGKRTREEMEKEHEAMKADPEMAQDISDQSKQTDHDKGVTQGQASSQDDGQSSKKVKME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X7C4</accession>
    <name>A0A8J2X7C4_ZYGB2</name>
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        <submittedName>
            <fullName evidence="8">BN860_12310g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_12310g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CDF88526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:cytoskeleton organization"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
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        <property type="entry name" value="Rho GTPase activation protein"/>
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        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GEF" evidence="6">
        <location>
            <begin position="572"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="1887"/>
            <end position="1919"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="7">
        <location>
            <begin position="1938"/>
            <end position="2136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="123"/>
            <end position="234"/>
        </location>
    </feature>
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            <begin position="1015"/>
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        </location>
    </feature>
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        <location>
            <begin position="1754"/>
            <end position="1782"/>
        </location>
    </feature>
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        <location>
            <begin position="761"/>
            <end position="788"/>
        </location>
    </feature>
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        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
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        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
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        <location>
            <begin position="123"/>
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        </location>
    </feature>
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        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="270"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="331"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="346"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="929"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1015"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1032"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1766"/>
            <end position="1776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF88526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2137" mass="238152" checksum="57A8E0442815FF56" modified="2022-05-25" version="1">MKGLIWSSKNKKSNSASSGSNGSSSSSNAPQSSNVASTKSRNANASTLLGSKSSNSSSTSHRYHHTSPSAASVHTTTSNVSSKRKSSPSAAFSLKRYSNAPTSSVQGTTKQGGTIYMADVNSRSNRSVTSAPRASSDLSLGSLPLESRQNKSQEKFHDAEMHQNSSKEHQSHAMASSTSNYSASTLQTTANPAPSAISSDITPTPSAASSGTNSNGHQHTSNLENTSRTPIHLNKDNYDQTVFRTGWLNRSHGQVQVSSGNESGNTGGSNRRDSKSFDHGFPEIPEPPVTVPDYKLYRVQLRGPLLNFYKSGLSNNVKSFDPNLGGKEQEELATDEEDVDLDDELTSQVTPTVATPTVSSTSISSASGPSRSSKLPTLKYLSPQHPHPDLKVDKEGKIVTGSMESLCHAILFAWSHTSLEDTSTSENRYLINLLLSLPILGDFYNYLAIFNQFGLTFTKHSGKLNSTSTQHCAVSPQIDDQLTDRLALVVKTLLDMFSSLLLDDEMLQQTMKLLDTIALHSSEISSNLKVAVTDKHIELSKLTAFSRGPVLTRSGNKPLADLMSPERFLRMDVKHLAEEVHDINLRFRLHWAPSTDYSLLYDSKFIDSSIVALNPLVFNNNKNVHFLGRLFISHLFPEGGSSSPRLRGKILSKWVEMGVRFEHLGDMVSWLAVATIVCSVPILRLHQAWQYVSEHTIRIIFKDWVPTIAQLNRRQLSSKSTSSVFILAPPNLEDPVIRSNVISYFGDLIIHADDLPPETKLKYLEKKINRTNNAFRKWQQRLDSLSKEGPEDIVKSPDNGASTTLLYQFWKYHLSLPPLSLSGLMELSTRMDPPRVDQKVYSSIGSQRSPLLTGSYLPVLFNELLPNYSLFSRQSLIGAAGVISTTMMATSILNPASVTSSASRRPNSPIGRNAQSITITGSVTMERGSNLSSNTAVSASDIGATSLTGPDEKTNGSQITGMENIDGPVVKAMSAKQCSRQHLLKCIRDAFNIDSDIFRVSEDFIFKSCNNDSDLTSGNSSTVLENPRRFSQHSSNAGTTGGTRESQEVANNLSKSLENIDFFNKEPETLKESVIPVVLKSGSLEKMFDLLVLTAGVFSRLVETKDLENYFHHNKERNISKSLSSDDNSKDENLGLLDFAFIRLSMDNETFTETLFNTYRSFTTTVNVLENLAKRYIGAKSCAVSITNLLNGSSKVKAPEAGAVNEQKFPVWDMKVSSDENLNYTNWSKIQVGAAESLYNLVKNHYADFTDDMASSNTLLDIMKIMEDDVGVEWPRRIEHMKNVASPEDVKETQRYFTKLSELFAGIRSAYQKQMYRPLGVNRTQRKVNSLLDSFRSVNLTDYNHLLNSNTLQDGMVAKFKELKYNDYEGILDWVYALDNMILQQMKLVTKQDWFVVYQIMELFSYESLTSYFSFPQHSAAYNMITSGSSQLDDLDIMNVFSWIRTLTRPNEKGNAHLLSQLPESVQLLVRLHTSLASFFTVEISDTAKNPDSRLQTCAVILQILNYVRWKNSTLDLFEEEGDSAPKFVSPHIPSFIETSISDAVVSPEARYFEHSWRAAHSLLSYKENDYLSSICRVLDNIDDKHLRAFVENDSIYMARPKNLCPCTGWLILRLIEIAQCVPNMSTMNSKLINFDKRRFVSNIISNIVDLVPVSEEYNSGANEPQGCNFGDSLSLEVMDPNKDFRKSTRIVAASESKMLKFQENGLFNEILSEEVEKIRRDQKKIETLSAQEHENKRAAVLQKVMQRKPKTSLMVPNLQPPSVPSSPVTGSSSSFARDKRSSVASMGARSSLVSNSGHNHVGRKLGGFFRRPFSIGGFNSSTSNNSLHSILVPDVQPNGTVSPSNLPVIDAVLVQEQKPVYQLKTFEVENVVELINHKNPAFVCSFKIIMQGGQEIVMQATSPAELKQWVRMIKASRRYAYYSKRYKSKTYYKVFGVPLEDICERESTVIPNIVVKLLEEIELRGLDEVGLYRIPGSVGSVNALKNAFDEEGAQSNSFTLEDDRWFEINAIAGCFKMYLRELPECLFTNEKVQSFANLALKLKAQQMTMESFTENMVSLLKTLPICYYQTLKRIVLHLNKVHQHVENNRMDASNLAIVFSMSFINQEDLANSMGSTLGAIQTILQFYIKSPQDFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X7K2</accession>
    <name>A0A8J2X7K2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin-related protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_15852g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X7K2"/>
    <dbReference type="OrthoDB" id="421448at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10221">
        <property type="entry name" value="ASKHA_NBD_Arp3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000050">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000006">
        <property type="entry name" value="Actin-related protein 3 (ARP3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF88677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="459" mass="50803" checksum="33B457D65EBF3014" modified="2022-05-25" version="1">MSYLNNPAVVMDNGTGLTKLGFAGNDSPSWIFPTAIANAASSNAKKTTSSSSGANLASTSTGGNAASSNSYFANATSATNFSNLTTSSLMSNNLSGKRGTEDLDFYIGNEALIAAQSTSYSLNYPIRHGQVENWDHMERFWENSIFKYLRTEPEDHFFLLTEPPLNPPENREQVAEIFFESFNCAGLYIAVQAVLALAASWTSSKVVDRSLTGTVIDSGDGVTHVIPVAEGYVIGSALKNIPIAGRDITQFIQSLLRERGEVDTSLKTAERIKQEYCYVCPDIVKEFNKFDRDPEKFAQYVVETQDKTRQKVVDIGYERFLAPEIFFNPEIASSDFLTPLPTVLDQTIQACPIDVRKGLYNNIVLSGGSTMFKDFGRRLQRDLKSLVNTRIAESERLSGARSTGVDVQVISHRKQRNAVWFGGSLLAQTPEFRSYCHTKKDYEEYGPGIVRNFSLFNMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X7V5</accession>
    <name>A0A8J2X7V5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ZYBA0S04-02916g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_02916g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC6 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X7V5"/>
    <dbReference type="OrthoDB" id="190098at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051601">
        <property type="term" value="P:exocyst localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.50:FF:000006">
        <property type="entry name" value="Exocyst complex component sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.70">
        <property type="entry name" value="Exocyst complex component Sec6, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010326">
        <property type="entry name" value="EXOC3/Sec6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042532">
        <property type="entry name" value="EXOC3/Sec6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06046">
        <property type="entry name" value="Sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF89380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="802" mass="93506" checksum="030DA0526F057113" modified="2022-05-25" version="1">MSSDILVKVSALVKDELSLEKIRDVKEQLIKEKSANEYELNKESEKYYRSVEDGLKLLNLSQRSVKEIRDGLDEVNKLSKSSKAAISRYDVIFEATKLYETIDMTSSIYDRIVKFRDLTSQLDQMLEYELSQDPLESGCPYLLQIHYRLTLARDFQDQMSVMADISTTDVQSTVRKLFAQVSVLVSKFDKLLESLIYDIVEFVRAEQISLVIRLFKIIDMEEREDLRIAAVRNIIKKKEIESEKSYMRKLPNDKNSARFHHGANDSKEYPSSQGLYEEILSGTVSTRTMVRGYKNFFFNKIKQSVQDMFVEVRKEYEGDKKFDVLNNLDWVFNELMVVKENVAKCCPEHWQIFDTYFKTYYEELHLLITELVDSEPETLVILDILDYDKYFQKTLVKDFGFSKQEKKSIIGDREKEHLFTDYLSLIVIKMSEWINNLEKSEFEVFYERKTPPHVDSEGLLYLDGTRTCFQMFTQQVEVAAGSGQAKILVGVVDRFCGLLKQRQKKWMDSLDVEVRRLINFNQKYDVDPESITPEEACPGGLVEYIVAVANDQMKGADYSVAISTKYGQMVTKNYERTITEEIESILDGFAEVAKYCSTQLIRIMFEDLHRPFEEIFSKAWYSGNQAQQTADTLYEYLVEIKLQMNVLVFSSLLESVVEETILRFIGALKYGHNFKFKNNKFLDCMKRDFEIFYKLFVQLVPETEDKTIIIDDKFKLMEYFMDFCCGPVDSIIDTWARCLEVYWDTPLVMLSAILNCRKDVDNSTTKRILQAATDVSNDPQRLSELRNAEVQPTFISRVDLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X834</accession>
    <name>A0A8J2X834_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S04-09956g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_09956g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCZ1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X834"/>
    <dbReference type="OrthoDB" id="240546at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013176">
        <property type="entry name" value="Ccz1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043987">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13056">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN CCZ1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13056:SF0">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN CCZ1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19031">
        <property type="entry name" value="Intu_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCZ1/INTU/HSP4 first Longin" evidence="2">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="548" mass="62468" checksum="0C317E33D97C1BAF" modified="2022-05-25" version="1">MFQYITVFDPTRSHGEDDLHQQLLLYHSFEGSENSLNDKLGQIGVIQGLWSLTTTLSEDPEVKEKVVELEKNILLVIHVESRFFISLGLAHDGSTPYQFYLSHLWLCYRFFTLWHGNFSSFEDARELTNLLNEYFVPFWIDLQATPGAIVRKGVASLWPEHHRVAELDFSPSDQSWESLINQDILLESESYLGIKDILVYHLPPYGRGPGNKTQGLVRHFSSDLDITSHISNWLYHSHAVYDTLSSHVLASNTHYKETPAEANGEVAANTTGETILHNLTLPITLAYDAVQEVGATTGISSSISLFMDYFPKWTTSPMSAMVANDTITESLATAPPYGYLISPLCGRSLPDNYKMRIVHSQMGTPNEKTYNCLFWYYRDVVVVIVSEPTFEKIWDRQYLEDLSYKLFRSIERFYATAFQADAHSKKDPFGYALLSKHSGELRCSIPSCPNMGDKIYSTFLDQLPQDKLWELQLELVQFLQSLQNSRRLHEASEERLLRLSNGVVCFLRETPDELALVVANWFDETSQKGSTLISGLGPAATRWWLNRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X865</accession>
    <name>A0A8J2X865_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S03-12530g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_12530g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X865"/>
    <dbReference type="OrthoDB" id="418169at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019417">
        <property type="entry name" value="DUF2415"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991:SF9">
        <property type="entry name" value="DUF2415 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10313">
        <property type="entry name" value="DUF2415"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2415" evidence="2">
        <location>
            <begin position="397"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="555"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="723" mass="80271" checksum="F57C6333EC0D23C7" modified="2022-05-25" version="1">MTIDGGQNSGDELEDKSLNTGSDKVYQNYMMPGLELYDAKVSINHWQLRDCVRASTRDPGKLYYIYDHSIRSLDTTQAGPQLASSQYRHHHNHHHKSSRLRRNSANCAKRGGASSSISRRSFHAPSQLLADFNFKPRCFTESQGLVACGGLVGPDDKGFPTNWSRLSQEINNGEALDAPAEPVKLAHGNVLADHSNYSNPSIWKGVLSLYNEDTDVASSFVLGQFINNCVTLHPRSTREYDLYSCNNDGHLYQCNIDNRGVELVRRYSDLKFALNNVAVSHDAKTMVASGDSNKFAIYRQTELAGQFSLSYDSQPGWGSSVVRAKRIPRFALPDGSGFVDHIYESSSGDHGFYNCFSENDLQFATLFQNGSCLVYDVRNMDTPLADITSTRPHSHNGAFRVCKFSYGLDDLLFISEHQGRVHVVDTRNFMNHQVILVPDKLKNDPTQGGTAVSTGLENEMEIIGETSRSRNGTPLIPAVSPSGSVASFAANASRRHSMAVPPSFNNLEPWLTSATQLPLRFLEPQIVPYPKALNKLTNFIFSQQQDDIPQDQLRGAISREGSSRRRSSFRVRRFSTSSNMSEGAGETLIDRPLMERQQQQQRSPSPPVDSYNYPGQRTGIFARGSSGSTFDAEDIYDSYADINSDGNTYSLTGAFGTPSMSNYAVDSAITYPDTDFTEENNIAGIDWVEDSSGSSLIIGTDYGIMKWNINSWARRSFSSYDFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8B2</accession>
    <name>A0A8J2X8B2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BN860_11122g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_11122g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8B2"/>
    <dbReference type="OrthoDB" id="366230at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010970">
        <property type="term" value="P:transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF22">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 INTERMEDIATE CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF87641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="586" mass="66123" checksum="8B5A04337AB87816" modified="2022-05-25" version="1">MDKHQELEEKKRQLKELRERRRNHEGYVHLLHSVQALDNPMVSVSIQTDASEELESSSVRAESKNEIITYEKGIQVDLMEEFQELPSSVLAPAQFTSKEPPEPIKELQLQEKAPSLPELIKLDPLVVEDQQNSIHNKSFSILEVLKESGHATLTSSSNSDFQFEHALTWDSKIMRDTESSTVCVSLDWHEQLMVVVFRSTPLQKFSTIAISWSHVRVFKWDTDQLIDSIDFRSQTLLAARFLRRKVKSNVISILLTTHTGKTMLYELTCTGGDRSKTIIRNLLIKNYHAHAIYAFDEYVDVPLGKERFLVASTNGVISELSSLDLSTYKDATANTQPIAQVVVQPPRASEICDLDKSRNEEGNEEPEEARLQCFTDHLLRVALSDELAITSLTISPSNQDMLYLGTEDGGIYRIMLTEIDNNKIKISRENHGFLPSESAPAFHSSHVVALAYNTQELLLSASLDWTCCLWDPKNNCKLSSLDVGTPVLAVQWLEDNRSAILTCDAFLVVSWQISSYVDRHTRLQRFRCTAPPLIQSQLTSEDAQVQYFTCFKAFPAAASNVVAIGSNTSFVKFYHVPNVEEVLANG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8L2</accession>
    <name>A0A8J2X8L2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BN860_16116g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_16116g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8L2"/>
    <dbReference type="OrthoDB" id="10250260at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000001">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_A">
        <property type="entry name" value="Ribosomal_uS8_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047863">
        <property type="entry name" value="Ribosomal_uS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003115">
        <property type="entry name" value="PRK04034.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF87860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="130" mass="14613" checksum="4390F944D2B81324" modified="2022-05-25" version="1">MTRTSVLADALKTIHNAEKAGQRQVLVRPSSKVVIKFLQVMQKHGYIGEFELIDDHRSGKLVIQLNGRLNKCGVISPRFNVKIGDIEKWTANLLPARQFGYVILTTSAGIMDHEEARRKHVSGKILGFVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8Q7</accession>
    <name>A0A8J2X8Q7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BN860_18316g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_18316g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8Q7"/>
    <dbReference type="OrthoDB" id="10266058at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12232">
        <property type="entry name" value="RRM3_U2AF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23139">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNA recognition motif" evidence="5">
        <location>
            <begin position="401"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="21"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF87957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="502" mass="57213" checksum="38444C0B35528D9A" modified="2022-05-25" version="1">MGVENNEQALEDLRSKIIASMGKNEASKESNKRTAGLNDGIQPSKKHRAIELPRGPRNFQPNHQPSRHPGDQPRATIRNERSRYQPQSRRPSSRPPSRPHGRYQGNIRQNNAFYTSMPLKFREEDLKDVVPIDERRRSGTTKWDLTPKGFENVPAERAKLSGLFPQPGKPQELDREKLKRVAADGGNENRRTRILFEDSRSNNLIFSELACKLIVAGISIPSLEEARQRLESFVNGLEGDHKLKGVIQNSNYLVLEFSDAICTTLVFSCKSFISELLGWSKCDWRRPHEYVQQLDNAESLCGSDIVAIERFPVSEEESVQKKLAELGIKPRFFKPICANDDSQVKQFTGCVLLELEENTSNLSGLEWFFPNKSCMTQKTSMVTFHGLPKMAIEPVRVPSRVLLLMNLVDPLDLKLEKFAEEVESTLEASLKNVECLRMKRPNVDFRLNFDHFREGVGNIYVKFQDVKSAEQALKWLSLRNFNGRSVLCCYVDESDFEISGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8Z8</accession>
    <name>A0A8J2X8Z8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ZYBA0S07-00452g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_00452g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="266718at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000196">
        <property type="term" value="P:cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000182">
        <property type="entry name" value="MAP kinase kinase kinase mkh1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000387">
        <property type="entry name" value="Serine/threonine-protein kinase STE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF48">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE BCK1_SLK1_SSP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="1161"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="217"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="449"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="628"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="696"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="723"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="773"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="902"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="945"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1035"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="223"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="312"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="739"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="798"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="837"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="902"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="948"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="958"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="980"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1066"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="1190"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF90415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1465" mass="162211" checksum="F6D7F089E39E32E7" modified="2022-05-25" version="1">MTFLKKITGSGSTHSRSTSSSSLKTWSQESPSSISNKHSSWLPPVSSKKRIQGEHRIPATNRNSYGYLDGAATLGRSSAEYDEQLGGSEESEDTSPYLWAAGASTAPWSEEEALISRKLSDGSSNSLSFDKLILSWDPTDPEEWNPQRVTSWLKFHEFPESWINLFRKYQLSGQRFIRLLAYDNFVIYEKYLPQTKNASYSRFQYLLKRTMKKNVINNHIRQKSADKARDVRSSSEYSKSKQHTNKSQEDVGSSRSASESALSPSNSGPNKAEDKTDKQVDKTTNSNNANAPLRAHQKPKSTGALYRRSFISIRGSSCGDSSSSKPPSSIRLNIPAAAHSASESKGESAKSLSPSSPAIFKRHQKSSSSESSLLNTLFGPTHNNSCTTEENSDKGSHLQVHNLSSESLPKSKIHLHETNSSSPLKQSPVISEEKSTLWTKWKRKSQISVTGNSLQIQPSPQPSIPHTSRFSADSPLSAKEQIPPAAITQGFPESPLPTANTVEHDNPITERVQENDNFNPSSYLLDKKFYPLRRKESINDTYILITKDNKTYLPLNLSMITSLEDLKDSCTFLLEIYHKHITIHMTDFGCEIGSALPDDMLETLRSSLFLNTAGKLFIKDQLKNTNPKPVITEPADQNRPATIKPKNSVKSIGSNITTSTDEISVVTSSSDISALDAQNSEPGRRYPQTPNAYFEATFTSGHSSGNHDSKSSNEDVNYFNLKDRPVGHRSVTRPQNPRSSSSSHNKSSGLSEKDRKGTFHVLRKDLGNEIDFNKRRESPYVQPELAPKREAPKPPTSGSPQRSLSISSQVSSSHLRRDSSKIHRTKRAQKARPPPSQTKASNEETSPLSDSVVNSYTPGSSHVLVPQPYKGASESPSRSKTDDDQYSAASLLAKQRLGRSDSFASATNSSHHTASPLLKRGSSKRIVSSASAADVFEENDVTFADAPELSDSDDSSDESSSSGTIIWSNGKQSSNNSQKTAVNSQTQEMNDVKHVSSDAINTLENGLERKMTLRPSPEVVYQNLEKFFPKANLDKPVLEGVTPPTSPKGDDNYVSSNVLAGLKTKAPSNSPDTNGRQFGSPSSNDLDRKQLNNPKGTRFPRRAKTIRTIAHEASEKRKQSMKLKRQNTKMWGTRTIEVTDKRLVSINKAKNSKGEYKEFAWIKGEMIGKGSFGAVFLCLNVTTGEMMAVKQVEVPKYGSQNEAIMSTVEALRAEVSTLKDLDHLNIVQYLGFEVKDSIYSLFLEYVAGGSVGSLIRMYGKFDETLIKHLTIQVLRGLSYLHSRGILHRDMKADNLLLDQDGVCKISDFGISRKSKDIYSNSEMTMRGTVFWMAPEMVDTKQGYSAKVDIWSLGCVVLEMFAGKRPWSNLEVVAAMFKIGKSKSAPPIPDDTLPLISRNGRDFLDQCFKIDPEERPTADKLSSHPFLNVGSKYDFKSTKLADFIKSNDKLNSTRLRIDSQEKNPNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X985</accession>
    <name>A0A8J2X985_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CCT-theta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_09494g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X985"/>
    <dbReference type="OrthoDB" id="1748577at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03341">
        <property type="entry name" value="TCP1_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000008">
        <property type="entry name" value="T-complex protein 1 subunit theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012721">
        <property type="entry name" value="Chap_CCT_theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02346">
        <property type="entry name" value="chap_CCT_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="571" mass="61897" checksum="F3558C08C9F8D4F0" modified="2022-05-25" version="1">MSLKLPQNMNSNLFKQGYNSYSNSDGQINKSIAAIREIHQMCLTSMGPCGRNKVIVNHLGKIIITNDAATMLRELDIIHPAVKVLVMATEQQKIDMGDNTNLVMVLAGELLIVSEKLLALGLSAVEIIQGYNMAKNFTLEELDKMVVGDIVEKDSQTELLKVIKPVLTSKKYGSEELLSKLVAEAVSHVLPRTAQGITPYFNVDSIRVVKIMGGCLANSTVIKGMVFNREPEGHIKSLPEGGKHKVAVFTCPIDIANTETKGTVLLHNAQEMLDFSKGEEKQIDAMMKEIADMGITCVIAGAGVGELALHYLNRYQILVLKVPSKFELRRICRVCGATPLPRLGAPTPEEIGVVETIKTMEIGGDRVTVFKQEAEESTRTATIILRGATQNNLDDIERAIDDGVAAIKGLMKHNGGKLLAGAGATEIELVSRITKYGERTPGLLQLAIKQFALAFEVVPRTLADTAGLEVNEVLPNLYAAHTVISGEGDEEIAKRDCLFKGVDISKDSSEGITDVREEGIYDMLAPKKFAINVATEAATTVLSVDQIIMAKRAGGPAPPQARKPGSWDQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X988</accession>
    <name>A0A8J2X988_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="15">ZYBA0S07-01904g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_01904g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPG/RAD2 endonuclease family. XPG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X988"/>
    <dbReference type="OrthoDB" id="31113at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09904">
        <property type="entry name" value="H3TH_XPG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09868">
        <property type="entry name" value="PIN_XPG_RAD2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000057">
        <property type="entry name" value="RAD2p Single-stranded DNA endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000061">
        <property type="entry name" value="Single-stranded DNA endonuclease (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001044">
        <property type="entry name" value="XPG/Rad2_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-G CELLS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171:SF7">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00066">
        <property type="entry name" value="XRODRMPGMNTG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00842">
        <property type="entry name" value="XPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="14">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="13">
        <location>
            <begin position="752"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="373"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="500"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="556"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="649"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="118"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="563"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="661"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF90476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1004" mass="115288" checksum="28DC5493A4092F04" modified="2022-05-25" version="1">MGVHSFWDIVGPTARPVRLEALQDRKMAVDASIWIYQFLKAVRDPEGNSLKYSHIIGFFRRICKLLYFGIKPVFVFDGGVPALKLDTIRQRRERRQGKRDSATVTARKLLAIQLQKQQETEKTNNTGKKEDNGSDDTSELFRPQDEWHLPHIPGFKYDVDDERVVSANNYEKIVNSMDDELEGIDLDSINPRSAEFDELPKSTQYMILSTLRLRSRLRMGYTKEQLEQLFPDSMDFSKFQIDMVKRRNFFTQKLMNVTGLHDGGASNLDQEVVNRISGQLHKEYKLTKTDSGWSLGLGELDGSETNKAILLDDKDVETLKSFGQKVSMKPESKDVDEDQDEENFEWEDVDIKPQHGTYVEDYSLNAARLPQLSRESSSAGGKSFLDSRPAQASPMKRLYGTPGVDTVEEEDDERSTIFTSKNENNIMTEDSRKLKADKHLMNVSQSSAGPFVDQNTDEDAEEGYLQQIEEIELMEAIQRSRRAEHIRNEKNSTMVEANASQHDHKNQIDPHQSIKKSHEPGKPTFATNKKIPTQSEQNLNAIVSKIPEFSLGSGGSFLFQGTEHPDSKTGNELAERKPLPETPTWFRNIAGEATNPFATSNFVQDREVVGSDAGNTNDILQRVTGLEEGQQIEVTRGSGQDDELLELQEQDINDGTPNDKQSSDLDQKNNEGIFDRKPLVFDYDFSEEDEEYIAENMRKEEREFSTFKNVLNNKIIDNAFLEDDLYEQQTKDKRDSDEVTPEMIQDVQDLLSRFGIPYIIAPMEAEAQCATLMRDRLVDGVITDDSDVFLFGGLKVYKNMFSERNYVEYYDYESIYKNLGLDRIGMIELAQLLGSDYTNGIKGMGPVSGMEVIADFDSLADFRNWYNEGQFDKKKQEQDSKFRKDLRKRLIKNEVVLDVNFPSETVKNAYLNPEVENDKTEFVWGTPDLDMLRAFFKRKVGWPQEKTDEVMIPLIRDINKRKKEWKQRTLTEFFPTEYIQGQRKLNLGKRITNASGKLKRRKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X996</accession>
    <name>A0A8J2X996_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_03268g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="2"/>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X996"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000003">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF8">
        <property type="entry name" value="HECT-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="7">
        <location>
            <begin position="500"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF90210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="849" mass="98613" checksum="EBCDB2EE4B1089E6" modified="2022-05-25" version="1">MPQLFRKFSRKRSPDVKLVSYSKASAINQPNRDLQRESKCSEDSPRKYSEAKCPCCGSFMQFPSNVKKLKCSVCQVTTELPDERNETTAPSVKPTSELRSVCTFSELQAITELCNSKRSETQATTKEELYAIFEPVGTYLNEKFHDIDTLNNSFKSAKKHELINYNELAQFYQLTMNMPTRSPFYKMLCASNDLLKKHPSSETDFCWILIIWANPSIRKSLTSQRRTCYDAPKILAVAYELGKRCIGYLANLDSTEQTERLMRHLKHMPFDLFQTQVITVNLYITFQLSRVLYRDLKASGTPTTKCLANDENLSPTLTYFDTVLKEDKKDIRNLKNGKLSIVQDFKFKPFEYQGNWHIRCAAQLMKILGNANDKRYTILETSNLPTCEFYNMMLDFIDFKQDFENWCADRRRSCNDAAAMLTETPAYCTRRITLCSFPFLLSLGIKISIMEYEVRRIMEYEAENAFLTSLDEGKAVSVYLRVKVRRRHITSDSLRCIRDHQKDLMKSLRVEFVNEPGVDAGGLRKEWFLLLTRSVFSPLNGLFNYITESRLSWFAISPINDAPHEGSPNNGQLYYLFGTVVGLAIFNSTILDLQFPRAFYKKLCAEPVNFNDYLQLYPETGQNLIKMLNYEEDDFTDVFGLTFEITYRDFNKELLGILPNVTTMELCPNGKHKLVTQENKHEFVQLWQDFFMNKSIESQFKQFVSGFRQVFALCESIKLFDHEELERLVCGNQEKNCYEFQMLRSVTIYVGGFTDRSQVVSWFWEIVQSWNFQLQRKLLQFATGSDRVPPGGMSTLPFKISRLGCKDSNKLPLAHTCFNEVCLWEYSSKQKLEHKLWWAVTQSEGYGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9C9</accession>
    <name>A0A8J2X9C9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone-lysine N-methyltransferase, H3 lysine-36 specific</fullName>
            <ecNumber evidence="3">2.1.1.359</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">SET domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">BN860_05842g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60324"/>
            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:15536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.359"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.359" evidence="3"/>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X9C9"/>
    <dbReference type="OrthoDB" id="422362at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140955">
        <property type="term" value="F:histone H3K36 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19172">
        <property type="entry name" value="SET_SETD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000033">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1740.100">
        <property type="entry name" value="Set2, Rpb1 interacting domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006560">
        <property type="entry name" value="AWS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025788">
        <property type="entry name" value="Set2_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050777">
        <property type="entry name" value="SET2_Histone-Lys_MeTrsfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044437">
        <property type="entry name" value="SETD2/Set2_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013257">
        <property type="entry name" value="SRI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038190">
        <property type="entry name" value="SRI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22884">
        <property type="entry name" value="SET DOMAIN PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17907">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08236">
        <property type="entry name" value="SRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18507">
        <property type="entry name" value="WW_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00570">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00508">
        <property type="entry name" value="PostSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51215">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51568">
        <property type="entry name" value="SAM_MT43_SET2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AWS" evidence="18">
        <location>
            <begin position="52"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="16">
        <location>
            <begin position="109"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="17">
        <location>
            <begin position="233"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="476"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="634"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="506"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="634"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CDF90321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="679" mass="78529" checksum="6858B4E338D7E2CB" modified="2022-05-25" version="1">MSSRSLSPPKGAIPPVKLFTDEEDKTQEALKTFKCLDECQYANKRLGNAKHNEFMECDCYEEFEDGTNRACDENSDCINRLTLIECVNGLCGSCGDDCQNQRFQKKEYADIAVFQTKLKGYGVRAQADIESQQFIYEYMGEVIPENAFRDRLIAYDEKGYKHFYFMMLQNGEFIDATVKGSLARFCNHSCNPNAYVNKWVVAGKLKMGIFANRKILKGEEITFDYNVDRYGATAQKCYCGEPNCIGFLGGKTQTDAASLLPQNMAEALAVSVSMEKQWIKMKKSEGQKVVKSEGNNINVEFVESLDPPPCERPEDVTKVMSVLLQVEDNLLAKKLLQRLFNIEDDTSLHHQIIKLHGYTCFGKLLQFFRNDLEMEAKLIDFLLMLPKTTKNGIITSHIDKEVDQVKQLHKALAEPCDKLLAKWSDFETYKRITKKDIKEASNRMVDLRRIRLPPGWEIVHENGKPMYYNAQLKTKLHQPPSGSSKTFNTRRQPTNSNANKSSMKRSRLDDGEYERQKLRRLEKEKEAIEKVKEEETRALKLRLEMENQKKSELEKIIAEANKLKDIEREEQAQLQKEKEEKRLKKKNNHLEHKWNKFFASFVPSLLRKYEQQDALSHEHAKQCARDIVKILTSKELKKDGAKAPPEEPSKDKRTKVKQFTASYMSKFLLKYRKKHTSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9L5</accession>
    <name>A0A8J2X9L5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">BN860_16226g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_16226g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X9L5"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.20:FF:000002">
        <property type="entry name" value="Initiation-specific alpha-1,6-mannosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039367">
        <property type="entry name" value="Och1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834:SF11">
        <property type="entry name" value="GLYCOSYLTRANSFERASE HOC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="68"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF88693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="395" mass="45445" checksum="04FD2DC54AC9E935" modified="2022-05-25" version="1">MAKSNQGIALKRLLLFIVPAIGFIVLLFRFFNNSKAADLHKLLQNLPKEISQSINSAASHQKSDSDLIQQFENLANDLRRQQEDQAREFDRHRQELEKKLQELKLSPAQATLREKLSYNFRYDERKRFPAFIWQIGGVSGIEQLSSLEEMEKNWRDKNPGFVHEVITDEMMNALVRHYFASVPDVVSAYNALPSKVLKLDFFKYLILLARGGVYADMDTDPLQPVPNWIPENLDPGNIGLIVGVEHDADSPDWRSRYVRRLQFGTWVIQAKPGHPVVREVVAKVTEATLQRQREGSLNINLRNDLNIMGWTGSGVWTDVIFAYLNDYLRSGNTEKITWTWFHDMKQPRQVADLLVFPAFSFSAPAEIKNDDDRKALFLTHHEAKKSWKSVPKVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XB78</accession>
    <name>A0A8J2XB78_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="10">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_00716g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the phage and mitochondrial RNA polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="10"/>
    <dbReference type="EMBL" id="HG316468">
        <property type="protein sequence ID" value="CDF91882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="276422at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001018">
        <property type="term" value="F:mitochondrial promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006390">
        <property type="term" value="P:mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1320.10:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000041">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.280:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1320.10">
        <property type="entry name" value="DNA-directed RNA polymerase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046950">
        <property type="entry name" value="DNA-dir_Rpol_C_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002092">
        <property type="entry name" value="DNA-dir_Rpol_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037159">
        <property type="entry name" value="RNA_POL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029262">
        <property type="entry name" value="RPOL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00940">
        <property type="entry name" value="RNA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14700">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01311">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00900">
        <property type="entry name" value="RNA_POL_PHAGE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00489">
        <property type="entry name" value="RNA_POL_PHAGE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase N-terminal" evidence="11">
        <location>
            <begin position="371"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF91882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1310" mass="148597" checksum="282C631D9982B669" modified="2022-05-25" version="1">MLRLPTRTICKASLIPKRLISSKGSRLQKPVTDLMTATSSSGLDVGQGLGFMKSSQVSMSPGFDPFDRSHAAALSQTTQSRDVMQLWSLLEACLCSKYYNRAMSILKSLYMVPSHRPYFIDDYNMYLESLVKSGVMNSIGQLDKKLSDDLQNTFKDVDYNDKTLAVMIHHALKYDHNLESTRNSLKTYFRMSVRGPRGILSHVNVLTVADYTALYADLSIIRFNDLPEAMKPLLEKEMQYKEGSLSSGLEAQPNDFKTANSTYEASKHETAEPGEKPCFSLGEVVQPLDKDAATLKAVDTLNMRVVRHALLGLSLNDQQREQISKFNFDANSNLLKSEDNKNLNFFEIYRSLKPEEEKDRFEKVLDDFNQERQRALESRATDAAKERWRHDFEEAKARGDISIEKKLNVKLWKWYNAMLPLVKEEVKLCSRVFSPSVDMIKGVPRQDAKQYKARLDYGPYLSLVDPEKMCVITILELLKLNSTGGIIEGMRTARAVIAVGKAVEMEFRSEQLMKSESQAFKDIGKNSHELKRYLQRAKSTFRSMKIEESKIMWPQTARAKIGSILISMLIHVSKVEVEGVDPVTKQTVYGEAPVFAHGYQYHNGSKLGVLKIHRSLIHQLNGERLVASVQPQLLPMLVKPRPWKNWKSGGYYYTQSTLIRSKDSPEQIAYLKAVSDANAIDTVYDGLNVLGETPWTVNGPIFEVMSKVWNSGKPFLDIPGIQEKLTLIPPPPREADPSIHREWKLKNKELANKFSSNRSVRCDSNYKLEIARAFLGEKFYFPHNLDFRGRAYPLSPHFNHLGNDMSRGLLLFWNGKRLGSEGFRWLKVHLSNLSGFDKASLSERVEFADRHIEDIKRSAENPLCEDAWWTKAEKPWQALATCMELNQAMKMENPEDFVSHQPVHQDGTCNGLQHYAALGGDVDGATQVNLVPNERPQDVYSHVAKLVIARLENAAAKGDEVANVLKDKITRKVVKQTVMTNVYGVTYVGATFQIDKQLSHIFNDRKYSLELSKYLTKHVFGAVRELFQGAHLIQDWLGECAKRISKSIRLDVDEKSFKNGNKPDFMTSVIWTTPLGLPIVQPYRETSKKQVTTNLQTVFISDPFGVNPINARRQKAGFPPNFIHSLDASHMLLSAAVCGKNGLDFASVHDSYWTHACDVDRMNVLLREQFIKLHEVDLIERLKNEFDERYKNYVQIAKIERNSELARKILAVRAELSKSLGRTATLADEIYMEKTRVQLLCSENLSEKEEGEKMVTSVSLVESIGGIEDVESKKSDASALVILTPLRLPSIPPKGNFDVKELKNSKYFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XCR5</accession>
    <name>A0A8J2XCR5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S08-05138g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_05138g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316461">
        <property type="protein sequence ID" value="CDF90871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XCR5"/>
    <dbReference type="OrthoDB" id="48509at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00072">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051640">
        <property type="entry name" value="GRB10-interact_GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003169">
        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445:SF36">
        <property type="entry name" value="FI03272P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445">
        <property type="entry name" value="GRB10 INTERACTING GYF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02213">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00444">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50829">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="2">
        <location>
            <begin position="139"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="572"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="224"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="442"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="484"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="572"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="588"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="602"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF90871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="780" mass="86026" checksum="3D615E9676AB6BAA" modified="2022-05-25" version="1">MNFQSQYQNPLNSMHYQLQNLSLQNGAPLDPAISQIGTPVLPAQPLSRTASLMDSIGIQRSSSPFVDPPSGLNTGHMLKPIAHKHDPHHPHPQDSTSSSPYPSQVATPNILHGGLFTGTASMAPPGVAAAPTVPPLVVESQWRYIDTQGQIQGPFGSGPMSQWYASGYFQPSLQVSRMGTSLEPFGINDRFMSLGELISKANNLQDPFHAFDLIASSMLSGMGSSFGGSPEQQQFQVQKQQELESKKSLGSADYTHDEILQLRDSDGGYYRDVVVQVPTGARKQERIEDRNIQARLEHKKKEEQRRRKEEEIGRNQMAEEAAKQTLQQESELKKKDVALKSQQEREPKEIAQHIVQTAPKASPKETMETAVWENIKSPKETPKASPIDISNETLNESSKANAKETSKSGPKTTSKASPKASPKANQKTSKTASKALAKENFQGQEEAAKQKASQQAQEVKTSIAPWADKVKNAEMPKIPSITELQKKEEQERAERELQERVAADRLKQQIIKEDKERQGLKSVLTWADKPKPPPVNSVNIKPQVKKEDKKKPSQIKSAVPLEEFNNPTFLKEQAKLWEEAQKSKSRRTATPGAPSGSSSASNAWTTVTSKSAKQVTTNQQKPTVQPKSYISPDKLRAIGASSGPNKQIGSSTSIPGLKATQAARAPTYPGNASISARQVFLRWCRSHMKLNASVRTDAVLEVLLSLPAGNESIGIITDTINSNSNAMDGKRFAEEFIKRRQECEKHIEDPLTWAEVLSMPEGDDDDWEFQVVSKKKGRKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T0H2</accession>
    <name>A0A8J2T0H2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BN860_14796g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_14796g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T0H2"/>
    <dbReference type="OrthoDB" id="407410at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051359">
        <property type="entry name" value="CaCA_antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12266:SF0">
        <property type="entry name" value="MITOCHONDRIAL SODIUM_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12266">
        <property type="entry name" value="NA+/CA2+ K+ INDEPENDENT EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="499"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="529"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="641"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="671"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="8">
        <location>
            <begin position="38"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="8">
        <location>
            <begin position="529"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF87801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="692" mass="77377" checksum="AFB7ADCE0DD630B4" modified="2022-05-25" version="1">MQIYQRRAVAALFYSINLAILVYSLRCESLWQFYTEPVVLIMCFVSLGFIASDFLTPSLSQISRGILHISDRVSGLTLLAMGNAIPDITSTYQAMNAGATSLAIGELLGGIFFLLTVVLGSMTLIKPIELKRMDRLSYSLKVPGGSLDTNDSTSAVYNRQLFLQDVSIFAVLILLSIYFLHDGRLMAWECVVMILAYCGYVMFLILDHNRDKLVPPNSGVIAESENVNNMTDISTVLSNPEALDPSQHGNMILFNEGIKERRANIRKRIRQYLRVNYNRWVRITLKDFLDIWENEAYLQNQEDLADAAGDVESQVGHDCISSLALIRRTNSWQGGDCPRDIPKICSTRVDNHSIAGNIDSSEPQENNTLLSVPAKRAVCKSLSCDHLPDLRSSYSIPSPLSFSSHETIECQSQVTIHRNFKTWVRSFRLSDYLADGNTYLPATEFTMLLFTTPIAILLNILIPVDSHSKNDQEFHLFDVVRLSLVPATSTLLLTEECPLWVFAMCFLLFVILTVRWYKSIVRHNMNFRAVAVFVLSLCAISSSVHMVVNVLMEWGEKFNISNTILGLTVFAWGNSLGDLVSNIIFVEIGVLDIALGACFGSPLLYFLFGIGVDGLMLMMNRKNDCGGSRMMCEITFQVDSHLGLSGLGVFMAFVILGVVVPINGWRIDKKVSISLLMLYVIITTINIYKEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T0L7</accession>
    <name>A0A8J2T0L7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_15126g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_15126g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T0L7"/>
    <dbReference type="OrthoDB" id="1667587at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF87816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="445" mass="50357" checksum="5CB0F2573C097020" modified="2022-05-25" version="1">MNVRHTIVENSVQKPPKFLHVSFNQDDSCFSTSTEDGFLIYNTDPLSCKLTKRFEQQDDSRSGAGAGIGYTRMLYRTNYTALVGGGKRPRYPLNKLIIWDDLQQRESIVLKFMSPVKQVFLSRIHIVSVLDSSIEVFQFQPNPKRICPSLEIFPNGPVDFVVSQLHRKNSQEPTDSTGASSNSSTGINGMLAFPSARSIGQVHIADFSRLKHNDQNPEGTQLLPTSIIKAHKTPIRFLRLNHQGTMVATCSIQGTLIRIFSTHNGSLIKEFRRGLDRADVYDMAFSPKGTRLAVVSDKRTLHVFQISLKEVHAGPGANDETSKNKLHALKNVVPSGWKPKYLDSVWSMCSVHLKNPTLKRSGVEPQYQQDRCKIGWCRDSGDHGTEFDEEDSIVLVWRKTGIWEKYVILEKEQQQMYSAQETLKTDTGKGSKTQWELVLESWREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T1G0</accession>
    <name>A0A8J2T1G0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="23">BN860_18382g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">BN860_18382g</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T1G0"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010106">
        <property type="term" value="P:cellular response to iron ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033215">
        <property type="term" value="P:reductive iron assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13877">
        <property type="entry name" value="CuRO_2_Fet3p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13899">
        <property type="entry name" value="CuRO_3_Fet3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000024">
        <property type="entry name" value="FET5p Multicopper oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000025">
        <property type="entry name" value="FET5p Multicopper oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044130">
        <property type="entry name" value="CuRO_2_Fet3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF361">
        <property type="entry name" value="IRON TRANSPORT MULTICOPPER OXIDASE FET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0186">Copper</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0410">Iron transport</keyword>
    <keyword evidence="15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 19" id="KW-0732">Signal</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035195836" evidence="19">
        <location>
            <begin position="18"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="559"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="22">
        <location>
            <begin position="27"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="20">
        <location>
            <begin position="155"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="21">
        <location>
            <begin position="362"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="606"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="622"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CDF87959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="646" mass="73090" checksum="B724BF541E3E7F5B" modified="2022-05-25" version="1">MIAALALLLLCTQQVLGATRVYNWTTGWGYANPDGIKSRPIITCNGQFPWPDVRVTKGDRVVINLTNGFNNTNTTLHVHGMFLKGETQMDGPPFLSQCPIGPNDTFTYNFTVPDNVGAYWYHSHTDAQYEDGMRGVFIIDDGENNENFPYEYDEEMVLQIGEWYDRTVAELKPAFMNYDNPTGAEPIPQNLLINNTRNLTWNVEPDKTYLLRIINTGGFVSQYFWIEDHDLEVVEIDGVQTEKMTASMLYITVAQRYTVLLKTKSSSDKNYAIMQKYDDTMLDVIPNALELNQTSYMMYDSSKTKPNQSYVDSLDDFLDDFYLVPYEKQELFPEPDHRIELDVVMDNLDDGINYAFFNNITYVAPKVPALMTALSAGKYATNSYIYGTNTHTIILKANETVELVLNNQDTGTHPFHLHGHVFQTIVRAPAVDDSDSPVAYNASNHTSFPKYPMRRDTLYVRPQSNFVIRFKADNPGVWFFHCHIEWHLMQGLAIVLIEDPLTLQKTASQQLTENHKRVCKNVGVAIEGNAAGNTTDWFNLMGQNVQVKPIPGGFTARGYVAFVFSCVAGVLGLITITIYGLMDLQNVEERVIEDLDISPAELLEEDDEEIEASRVCATSKRSSLEEQKIGEEQKIGEQETVRPLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2Q8</accession>
    <name>A0A8J2T2Q8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">BN860_17524g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_17524g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glutamate 5-kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2Q8"/>
    <dbReference type="OrthoDB" id="409889at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004349">
        <property type="term" value="F:glutamate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901607">
        <property type="term" value="P:alpha-amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04242">
        <property type="entry name" value="AAK_G5K_ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21157">
        <property type="entry name" value="PUA_G5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.10:FF:000008">
        <property type="entry name" value="Glutamate 5-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000020">
        <property type="entry name" value="Glutamate 5-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00456">
        <property type="entry name" value="ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041739">
        <property type="entry name" value="G5K_ProB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011529">
        <property type="entry name" value="Glu_5kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005715">
        <property type="entry name" value="Glu_5kinase/COase_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019797">
        <property type="entry name" value="Glutamate_5-kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01027">
        <property type="entry name" value="proB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654">
        <property type="entry name" value="GLUTAMATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654:SF3">
        <property type="entry name" value="GLUTAMATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000729">
        <property type="entry name" value="GK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00902">
        <property type="entry name" value="GLUTAMATE_5_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PUA" evidence="10">
        <location>
            <begin position="310"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF87922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="416" mass="45336" checksum="F472488D7EB75667" modified="2022-05-25" version="1">MSRPYTIVIKLGSSSLVDENTKEPKLSIMSLIVETVVSLRRLGHQVIIVSSGGIAVGLRTLGLEKRPKHLAEVQAIAAVGQGRLIGRWDRLFSYFNQRIAQVLLTRNDIAQWSQYKNAKNTLHELLHMGVVPVVNENDTLAVSEIQFGDNDSLSAITAALTGADYLFLLTDVDCLYTDNPRSNPDAKPILVVPDLTQGLPGVNTDSGSGSDVGTGGMQTKLIAAELATNAGVHTIIMNSGTPANISKIVQYIQSSEGQTYKGDLLDLQASELQKLQHLGVPLHTKFIASGNRHHLKNREFWMLHGLVTKGSVIIDNGAYAALTRKEKAGLLPAGVIEIEGTFQQLECVDLKLGKRLPNGYLDPDISLKTVGRARCNYTSSEISKIKGLRSDHIEEVLGYCDSEYVAHRENLAFPPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2S4</accession>
    <name>A0A8J2T2S4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_17854g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_17854g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2S4"/>
    <dbReference type="OrthoDB" id="4067996at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="573"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="386"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="949" mass="105841" checksum="B7D12648F4D78360" modified="2022-05-25" version="1">MDLEKPELGLMTTESNMRNMCSNKGADPLTGALINNGSEEIGQLQREVQIVSNKCKKIESLLLARDTDITGSLESLFATFRTVSHNQTVLENKLDDALKNQMNTDLMVNSLNERLNGLSEAVGQISKASASAAAAAAAAAQNNGNVASATPVVEQPISRPTSVRRGPGRPRKSASSGVGSRGSDVNGRQSPPVKVSLPLGNVQVPKSRRYFNDPKVKPEPSKPKEEPLSRQRAQQASVKTAVAVNPPPKRKRGRPPKKRIVETVIVPPKSNIENHDEIAEKNGKNAEKREEEEKTEELNDKTNSQKGDHHDGENGNEQDANEDQESLEDDDEDDQDYRNEDADPNYKEYSSKEESPQTIMPPDSKAIVSHASSPSSSRSSLPSQSGFIPSTVATADTTRPQGNDTFDELSPSASSNTTQVVLPAPRKRILGANNDGNTLPNSAGNQSESALSFFAETSLDSAANKLQRELQQRKLDKRRDSREKMLVSMKYNDRQKAKSFMESNKNLLQAMREEERRKRMTALIYDSGVGGAQQATLASSASNSKIPEHIFKLEDKTQNSAKKMGISTMLNGEDFATSLSSPPSPEGPKRRRSFDYFSDEPHTLRQFRRRRAASYLTTPTMAALENPSAGAVSNSLGSGNNVTAGGNGNGNGDILNPSSASGAKKVDSQASLLLASPIELLCKDGFFFRRNTPENPITVGTYLEFKFKPKEEELIGLTVSQKDYADRTRHDRMNAHFLKPEIQAETEFAFQVLRKTTLTEKYVNSLEYFVMEFRWENKLVGLGLKLRESKRTWQRRKALFSLFEFWRDQSREKRGFPDYTMMHAVKEMENYRIFINRSVSWFYNHITLLKMILYDLCDNVNSQWREWMYAKDSRLPVVGQDGVTEDNIDEKLDSVLTLDFLDDGTENSQMKFHRALPISERSTDNEADVDDLDDDAIQDRVENLSDVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2Z6</accession>
    <name>A0A8J2T2Z6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_10880g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_10880g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2Z6"/>
    <dbReference type="OrthoDB" id="4064247at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159">
        <property type="entry name" value="CENTROSOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159:SF31">
        <property type="entry name" value="CENTROSOME-ASSOCIATED PROTEIN CEP250 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="214"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="386"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="231"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="264"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="277"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="837" mass="96405" checksum="005DE5AE224D89FB" modified="2022-05-25" version="1">MPLLTSDYVDQQEVSTTALQGPQTPETSFHRHHNISNLEDYSIRDDDDEQTSSDDENRNSEDHENSSLKSWFFRPKNRCNSISPTKEASKNLSRLSSDSVIAEGTPCNSKTGSPFRKSLRSFFRREGSKELNANELKTSTSSGEAESQQQLDVSNRRFWKPWKGHHRHEDENIDSSRDKIKTLNVAQVDDNALLTHEQKRERNMEKLTQQLMDFPSSDEEERAVTEVQGCKLHETQDPRLSKAALHEDDESNYASSDSTSSSIERAKEVKERVGSVFSPSTEDNSPKQNDVSPLTPVDDVMDNPYKYVFEPAEPHASAPVAYDCSWIDKNSVAFDNFDRALKMLSRSSDCFSSLDGSLFTVSELSNEFLKLVHSTLDKSEKHYQAKKALEDTLKTIQDSYTHIEKEAESKNQEKTHLQKELEALKNKYSHLRSEFESLSEEAEILTDEIAQSKKNIFAAKDHERTSIERAQSEAATLKQELQEVKISLEQALADKKSLSANYHTRIAEQDAMRMQCKSLTEQKDILQRKIESLGKENETMRAEKACLQEEYESSKEMNRSLQITQDTLQAKYATMQAEYDELRSKNDVTQAKFDSIQNDYKTVVFDNEILQEKNNILTTRLSDLVGSMSKGENGERDMRNDLEVSGNKLRRYENIIELLKIGNLKIQDNFRAERNKVLDLRRENKVLRKQLQLTECYRSQSLQFMSHLMLYYRGIVTEETLATFNFHLKAITEFSFVTDATQDNLEVESKMKECETAIISFYTKVAKESFLDQIITKHVSYMRSNNFLSSQLSGLRKQISDYEDYVSRLLQEIETCKKVQEKNQRKISHLKAQNMEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T334</accession>
    <name>A0A8J2T334_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA pseudouridine(32) synthase</fullName>
            <ecNumber evidence="3">5.4.99.28</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_09780g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>uridine(32) in tRNA = pseudouridine(32) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42544"/>
            <dbReference type="Rhea" id="RHEA-COMP:10107"/>
            <dbReference type="Rhea" id="RHEA-COMP:10108"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.28"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.4.99.28" evidence="3"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T334"/>
    <dbReference type="OrthoDB" id="424794at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016814">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160151">
        <property type="term" value="F:tRNA pseudouridine(32) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02557">
        <property type="entry name" value="PseudoU_synth_ScRIB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000061">
        <property type="entry name" value="DRAP deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000017">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluA-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF40">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE RPUSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18785">
        <property type="entry name" value="Inv-AAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="6">
        <location>
            <begin position="450"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00036184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF87584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="600" mass="68267" checksum="8894C9DF3F4A21D9" modified="2022-05-25" version="1">MVTRLKHCSGLIRRMSSCGKNEPGNPHEFEKHLLKEIEDVKANQLIKNKRKRVQKLQTAKELRDKNGFKLKVADVNSQRHKQEDPEYEVIIDGSLRRISPYYFTYKTFCKLRWRDRNLFEVFVDEFRDRDRGYYEKTIARGSVYLNDTPADLKSIIRNGDLIRHQIHRHEPPVTSRPIKVVYEDANLLVIDKPSGIPVHPTGRYRFNTITKILEKKLGQTVHPCNRLDRLTSGLMFMAKTPKGADDMADQLKAREVSKEYVARVVGNFPSGEIVVDNPLKSLEPKLGLNVVCSMEDEGAKHAKTVFRKVSYDGQTSIVKCKPLTGRTHQIRVHLQYLGHPIANDPIYSNPKVWGPQLGKNGQADFDKVISSLNAIGRSESAQSWLHGESEGELMSEEKCPVCETNLFTDPGPNDLDLWLHAYSYESTELNPQTGQKKWSYETEFPDWVLEPHRKYMELALEQASHCEPTKTAFSVGAVLVHDNQVLSTGYSRELPGNTHAEQCALEKYFSEHSGEDLPENTIIYTTMEPCSFRLSGNEPCAQRILSTNGKIKTVFVGVLEPDTFVKNNSSRNLLQAHSIDYVKIPGYEIKATELATKGHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3H1</accession>
    <name>A0A8J2T3H1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BN860_12354g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_12354g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endomembrane system</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3H1"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="289"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="289"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="307"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF87695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="350" mass="39036" checksum="CCC1821DB69248C8" modified="2022-05-25" version="1">MSRKRLSGSHQLSPLSPVIPICPASSSPASPQVGSKSLSFSRQPRETSDIKLLLVGDAGVGKTAMILGFCGELPTKSQLQIMSLTKDSPCASVSGMQRMRNVTKKRYSLNDYEELFQSADELEEYVLDTKATIGVDIKTTLLNIDSRLFKCTLWDTAGQERYRNAMIPALYKDAHGIVLTYDLCDSDTLESISSHWMQEALDNCGSSDLARVRFYLVGNKLDLTKQRKVTQDQIIKTVAFLQSHYHINISGTFEVTCKWPNVVDRTFTTLVRDLVASGCYNTKKYSEVPQRRTEENKESDQSGGDDNSCKERDRGQGLRRHSTTEGKNIPGGIDLTRPYHIGKHTSTCCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3I1</accession>
    <name>A0A8J2T3I1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_12684g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_12684g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3I1"/>
    <dbReference type="OrthoDB" id="1936594at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044244">
        <property type="entry name" value="TTC27/Emw1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="628"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF87710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="893" mass="100653" checksum="4FD8D935B466DC12" modified="2022-05-25" version="1">MEAGLHAYILLAANPEQLKADFLKGFDETNLRYGRYILEGTPHLVVESILQETQPPSLSEEADERIILTSLKELAGRLNNSQALILSIALLQTFIQNNYTGPSSSINVLANVDVVQERLVNLLSSGGQPAYGLVERPVLLLLSLILLEQITQQPSIIATENEVDIDPPTISATETPSLLAVAHWWRARALLIHLSLLPEPSGFQPAIIASILGSIDLVHAITKELPKGFAEGAKKNLYIIFYLENVKASLAINSEHSCLPSLAKVKKLTNFDFVLTGARAKRTKFQEKAHSGLIILAESSYQSLEDEGNEQSDALPENLTLDSDTLLEKAHFESIGNEPLDQQIIKSRKLDETESLEQENVFPVAIRQENIPAQLKALNPNHQPRLSGYDDIQLLLRYYVLKNTSPAKDALVEEELGAVIYRIIHQDGVQNYSIFSRALWERSILETMKARTVERGLLQMQVLVEELGLKIKTRLIPHHDNVENPASRLKYIHQLPFIPRWVLDATLADKYMSLGVLKSAVEIYERLHMCCEAALCHAAVGDEKQAELILLKRIEETPKDARAYSILGDIRQDPALWSKSWEIGKYANAKNSWARYCYNPPPSSNLERNYDAVLKHLNDSLRQYPLSFDTWYFYGCVGLECGKMDLAAEAFSRCVSLDPTHGTSWSNLSAVYVNLGKLKESFSCLKQAISTDARSNWRIWENYLIVAFKLNEWKDVLMACVNLVTLKRDKLGEASIDLPMIEKLTELLVSSEYNEQSLSYFQRSCIDFICNTIPSVVTTSSRCWRVVAKVELWRGRPWAALDCHEKAYRAVSHNPDLEVDEKVWEESVDACDDLIAAYESLGEMQGRHGPGSVVCQDWKYKSRATIKTLMSKGKNTWEDSVGWERLLEMRGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3T4</accession>
    <name>A0A8J2T3T4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">AP-3 complex subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_16688g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Adaptor protein complex 3 (AP-3) is a heterotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3T4"/>
    <dbReference type="OrthoDB" id="10264595at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030123">
        <property type="term" value="C:AP-3 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000251">
        <property type="entry name" value="AP-3 complex subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017105">
        <property type="entry name" value="AP3_complex_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22781:SF12">
        <property type="entry name" value="AP-3 COMPLEX SUBUNIT DELTA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22781">
        <property type="entry name" value="DELTA ADAPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037092">
        <property type="entry name" value="AP3_complex_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="11">
        <location>
            <begin position="50"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="690"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="812"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="849"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="892"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="719"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="766"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="819"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="905"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="919"/>
            <end position="940"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR037092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF87885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="946" mass="107460" checksum="FA61D8CB2045F605" modified="2022-05-25" version="1">MPLLNAPTNEDVKLRLRPFGIFFEKSLKDLIKGIRSNNETPEKLQQFLAQALSECREEANSSDFTLKTNAVLKLTYLEMYGFDMSWANFHILEVMSSSKLQQKRVGYLAASQSFYKDPDILMLATNLMKKDLKYTGANDVVKVGIALSGLSTIITPSLASDICDDLFSMLNSTKPYIRKKAITALFKVFLQYPEALRDNFDNFAARLEDDDISVVSAAVSVICELSKKNPQPFIQLSPLLYDILVTISNNWIIIRLLKLFTNLSKVEPKLRPRLLPKILELMDSTTATSVLYESINCIVRGNMLEEGDHEVAMQCLDRLHTFCDSSDPNLRFISCVLFYKIGKINTAFISQFDKLVMRLLNDVDVSIRSKAIELIQGIVDENNLQKIVLVLLKQFVDEDVVVLQTGGHEATKEIPIFIPEQYKIKMVNSVVHICSMDNFANITDFEWYNAVLWDLAILCQDLSDKNLGYKIGEQLQSIMIKVPSMREITMTTIIKVLYSDELNTHLPGILRDSFWALGEYSSYIENGDDLIRLVLKRKQYYPANVNVVLIPTLLKIFSSWCNRPVELGRENVKKVLVDLLTHLEPLCYSKAFEVQERAVQAVEFLKLALEAMEQDTDGLPLLLTDVLPSFFNGFELKPISHGTQQKLQPNITLDCETPFLTEEELQSILNQEEQENNEILLSDDLLSVGSKSDNEQVHSDSSAEENDVMEQSPLDNTELDLRRKEERESNPFYLSREEDYTGKQNSHLLGSIGKEDNESENNMIRVNRDNDPENHMIRLNRDVDAVEKDNAGITKSRKKTKKRVHVLSDEVLMDDNPKSSSPSVRSLSTTASSARGKISLRVPTKLENFDFSKPTLSSQNESSEWKSEEVSEEQLELEKLRQKFEKQSLTINEAATGNDGDTVIVKKKKKKSNKKKGKHSADKKSNKVDESESSKDRKENSLNTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3T8</accession>
    <name>A0A8J2T3T8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_09802g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_09802g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3T8"/>
    <dbReference type="OrthoDB" id="10263032at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1040:FF:000003">
        <property type="entry name" value="N-terminal acetyltransferase A, auxiliary subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021183">
        <property type="entry name" value="NatA_aux_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767:SF2">
        <property type="entry name" value="N(ALPHA)-ACETYLTRANSFERASE 15_16, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12569">
        <property type="entry name" value="NatA_aux_su"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000422">
        <property type="entry name" value="N-terminal-AcTrfase-A_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="239"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF87585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="841" mass="97101" checksum="2A33EFD177EA4B53" modified="2022-05-25" version="1">MPPKRFLGSKNLSGKAREHTQFLEALKLYESRQYKKSVKILETILKKDSSNVDALALKGLDLLSMGEKQEAENYIKNAIGKIQGIGASAICCHVLGIYMRSSKNYPEAVKWFQTSLNNGSTNQQIYRDLATLQSQIGDFKGALASRKTYWEAFLGYRANWTSLAIAQDINGERQQAVNTLSQFEKLVEGKLGEAELYEHSECLMYKNDIMFRAAGDSEEKLQNVLRHLTDIEANVYDKYAILERKAAIYLKLGDAKQASKHYRMLLQRNPDNFDYYKLLEKSLGVQNDVTKREILYDNLQRLYPRCDPPKFIPLTFITDESKLETKFKEYVLPLLKRGVPATFANVKPLYKKKSSLIPAISEKIVLELYSSLNPFEDPIPCVWTCYYLSQHYLFLKDFSQAQFFIDKALESTPTLVELYIMKGRVLKAMAKLDAAADILEEGRKLDLQDRFINTKTVKYLLRANKIDKAVEIVSLFTKNDDSINGVKDLHLVEASWFIAEQAEAYNRLFIESQKQLHGTLEDFKNSGFKDTEIDSKLRELKQLKWNVEKYQGLSLKRFEAIAKIYRQFEDDQLDFHSYCMRKGTPRAYLEMLSWGKTVYQSPMYIRAMKGAAGILFQLQDKISTPNQDLQETFDTVMKENSKKSKKINSSLNKQREEEKKTVLAYAESEDDDIFGQRLVSTKKPLQEFWVEFYAKYSNQVSEDTRDYPLEFEYQYRTRKLALCLGAFSKYAGFHGENSGFAGAMAIVLLLATRENTPFETIAKKVAYKGCESIKFLPLAERDNDEFDWAAFFSQHFKKDLPSLLFLYHNVSGHRPAFKNLILEEISNSRPQLQMQVLQYQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3V4</accession>
    <name>A0A8J2T3V4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BN860_02806g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_02806g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3V4"/>
    <dbReference type="OrthoDB" id="289721at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031403">
        <property type="entry name" value="Sbe2/Sbe22_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053949">
        <property type="entry name" value="SBE2/SBE22_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053948">
        <property type="entry name" value="SBE2/SBE22_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17076">
        <property type="entry name" value="SBE2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22874">
        <property type="entry name" value="SBE2_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22876">
        <property type="entry name" value="SBE2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SBE2/SBE22 N-terminal" evidence="9">
        <location>
            <begin position="31"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="SBE2/SBE22 middle" evidence="8">
        <location>
            <begin position="383"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Sbe2/Sbe22 C-terminal" evidence="7">
        <location>
            <begin position="512"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="214"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="306"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="467"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="135"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="228"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="319"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="362"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="478"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF87279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="825" mass="92692" checksum="80650F0251AF35E5" modified="2022-05-25" version="1">MTSRLDTLKEETPPELPKGSRTRSGSSNGGSSRMMGLGLGRRPSDNLLLNMTSLEGLDLGDGIGGGSSGSGKAPWPPPPKKNFVMRGDRPISNDSIATRSTELFSSASSDVNSGSSSIDNEQDVKERTSETLALEQEAPQAQADESMISTDGEDLSPPCSQAAAAAVRDISSTGPPLGPQAGARNRFVFSNGMKAKSQIALKSASLHSENQLPTAAPQLPASSGLYGHSNSTSAILPSKTPLTPSQRYRLRKSQNETALRQSIRKKEKFYDEQEANTQLKNDNFESSLVWNVPMASFSTNSFLEGADVQASSHRSQDHHYRHQHNHHHHKQHSRNHSDPSFGGNANKDSAVAGGDSSGRSPLSHRKQQHNQRKGLPQPGSNLSFLNFYDMPTSPIPGLNGTSDFQYMQQASQTLSSVYENSSHTLSQSKLSERSYSADYLPMEFKTASDLGFEDLILVSDNKLEVTSHSRPSWLPPKSPEERKMHEEQISKSTSMASIEQLDRNKEKEERLLRNETNRQKYVLLLDRGITRNSSLQSLKKIIWETALTDEVRRNVYDEILQSDARAVTTHYLESYEDMSNLLEKMDFPGAKEKEIEQLIDAGVRNKLPGQEEISPELLPMLKMKSISRQGLQPGDELLFHHFLMSDSFKSSQEVWETANLVQMTCFNDLCKEKYDTRILNSRGIVANYLLRSDDFKDEFNPTTLNSTTWWNVLQRVSHGLFMWIMDIIVVANNQCYKKYPVRPDDYENKSWECYRSNRVVQNYKILLAFGLNVLLNYHFGFNDLKSLSSLDDPNFTIPMPLDHLLDADAINSMFIRKWLHYYKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3V8</accession>
    <name>A0A8J2T3V8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BN860_17744g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3V8"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF46">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="300"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="365"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CDF87932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="660" mass="75257" checksum="DA84C805D347AC69" modified="2022-05-25" version="1">MGEKGFRDENAASANDCIILDEGPQRGYMAADVLWNHRIGEVSICDGILALTVCLGFAVIMLKKNATKLTLDLHEDELELYEAVKYYKQGKFSLGPLGPLGVQLYSLGPLCRNFMRKVSLIFSSATLGTFYLGAKRAGISPVFALATVCGVAYLPLFQLQSLQVCLGPLQWFLLSIVFYSWESFTYFPQLSTKWFAHLLLLASSVGLSVSTKYLGFATWAWIFILVALHFWNTISDVQLSTCFVVKSTILQASALTLVPAIIFLISNILQLSHWSEDVPEFSRFMSSNFKAYLRGPIEHPVGLNYGSTVTLRHLESLGGYLHSHNYTYETGSFGQQVTLTQVEGDPDNEWIVENKFPDKNNAMLNKKIHDSDQIRFRHRRTGKLLRASTAKPPVSQEEYDLEVSGTGGFDYDGDSDELWTIRVVNAPRRSPVKPLESIINLQNEGHSCALLAHDIRLPEWGFNQQEVLCVEEPMESRTRFKFELVRPAVEVEEYETYYCKKGKISCIMELLFELWKRQIKYNSYEKNYKAISSYNPENWPFNLIGKKLADNIWVFGSLMPVFFVMYQLLQLLKWNPYEKKQEVSLNSTLLYKVGSECALGWLIHFYPFFKNPHSHLDVTLYLPALLLGQMLSAEIVDSLYKWNRWSLACIAAYLAVVIYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3W6</accession>
    <name>A0A8J2T3W6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_15918g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_15918g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3W6"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="7">
        <location>
            <begin position="316"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="71"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="242"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="667"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="144"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="249"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="731" mass="82022" checksum="C8D9000BDAE7C0AF" modified="2022-05-25" version="1">MTPDAPLAGFELPPDVSQVESENPLAVLAPETNAKSTGIMHDQVDQENSAQDPEPPKSKLVELLKAFEQPVDSERLPKKPVDADSKHPKMFKKIGIPEQTETLAEHKENEDNAESVVIEIEVGSDDPSNLENFEQSKRIEQSDESGSSESGEGSDSGDSAQSVESGQPGESGQSGQSDQSSQSTESCASVESDKPAEFTEITEAKDPGEFNRPNEIQSSNPVAEELDEMTRNEEEHHLVANRHFSPGSDYERACEHESPSSEQTDVSSEPDCLDDSVGACLPSVETLVIEAPNLIRGYNVGIDCLPMQRERMVARDGVHFTMMVAGKSGLGKSTFVNTLFDTCILPTIWQIGEDLSQETRCLIKYGAHFEAENANMHLNVIDTPGFGSKCNNTFDWVPLVNYIDEQIRSYIFQEEQPNRGNIKDHRVHCCLYFLEPTNKGLCALDVVAMKEISKRVNMIPVIAKADALSHDEMQSFKQEIREILHSQGIKVCQFLEESNSFYRDIFQVAPFGVVGSDMKVLDSNGQTIHVRKYRWGIVEVENPAHCDFSLLKDVLILNHMADLVRTTEEYYESCRASILKTRILKARDLASEGCLLEEERRALQELDYEQADNNGLKNYACYEAFNKKEMDSLVTECSPEFVQKRLEARKKFNEVINLEDQKFQEWKKALQNKQLKFNQEIELLNQSIEDLQLECHELESQIMTTKAVQRTRTKLSNISGTISKGDKRASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T404</accession>
    <name>A0A8J2T404_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_12706g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_12706g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T404"/>
    <dbReference type="OrthoDB" id="4066471at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028002">
        <property type="entry name" value="Myb_DNA-bind_5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13873">
        <property type="entry name" value="Myb_DNA-bind_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="2">
        <location>
            <begin position="27"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="270" mass="30797" checksum="95E9ED898D4289E5" modified="2022-05-25" version="1">MPDGLPFEDDDLLLELMDRYKPHLKPYAYRLQTWSQVLEQYNTLTDNHYRQIRTLKKKFERLKEVYEYDATKIQITDIKRLERLIQESEHVTKKTHSTPLERGQVRHITGEPKMPKTDTGSSSEVHTEVNADNGESSSDSSQPPPLDSITVGFPHSASLREPRNSVASSSSHRSTAPGTVLFEESTNEPQLQSQATPDDPNKTELLTNVLSILLDSASSAGIPQHNATTLEKLYVEFKQYKSSQEEFQRQLLQRLDVLTAAITHKEPFHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T407</accession>
    <name>A0A8J2T407_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">BN860_18976g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_18976g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T407"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF87984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="304" mass="33813" checksum="01446AD1A702A81E" modified="2022-05-25" version="1">MSSFRRNNEEHFWRRCCRKLIEMGDESSRAAERHHSIRRWKNVHHTGFNTCTSETTKHGTALIQNPKEQTFYEIQEWQQPAQQLRFKKSQRTVSGAATAPALNSGLNSTATLSPLTFVTAETTPTGKVISCSLNEDEPRPTLDPRSSCSFRSSIKSARFQKAESHQRKVEPSASFGIAVGASSAASSFLNNSSEEDTTMTCESTVSSRGSVGNVARSPSFDHIVTSFDWYTTGVFKKLETSVLSLLNLRDYYASDNSENSSSPVTMSLFNDFYGKNSTPCTGAASKQEQEIDEEIYGHHAKCFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T412</accession>
    <name>A0A8J2T412_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_13102g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_13102g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC10 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T412"/>
    <dbReference type="OrthoDB" id="125856at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009976">
        <property type="entry name" value="Sec10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048627">
        <property type="entry name" value="Sec10_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048625">
        <property type="entry name" value="Sec10_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100">
        <property type="entry name" value="SEC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07393">
        <property type="entry name" value="Sec10_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20667">
        <property type="entry name" value="Sec10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec10 N-terminal" evidence="7">
        <location>
            <begin position="55"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec10-like alpha-helical bundle" evidence="6">
        <location>
            <begin position="179"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="492"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="494"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="828" mass="94232" checksum="D7B6AD6C4A3F3E1F" modified="2022-05-25" version="1">MNSLYDLDPRWRKLLSPNNFLGGLTVNEFVEELSKDHSLRGADGTAETTFEKLNPKPYIRTFESILKELNVLSEQSSSRKAQLVEQVSAQELEHAENVVQLSQELKKTMYNYEKLDSQLTNVTQVVSPLGEKLEVAIRRKKAYIKSVELISHYNAFYTTGESKFLEDLRSSPNFQKKTQAALLVKNLLLLARKVETSSIPRTVETASNIEKYSEILETDLLESFNNAYRVNNFSQLNEIALILNHFNGGVNVIQSFINQHEYFIDTAQIDLDAGNQILLEEEFKGELVDSDKHGVIYEKTMVAHLSDIETVIKNESKVVKRVFENRALLVMQKFMQRIFAQKVEGRVDLLLSTAGSLSSLAYVRMLHALYSLLGQFTKDLADFFKLQEIDRDGILSTTVEQSFTELFSKYLYDKSRYFDIEKRSLESILIEKTTNFNITHDKDIRSRSLSGKLNNNLDSGFGLHEIGSSSKNKFVQLNNFFKSRIDRERLTFGRNNSLRSSNPPSSDNSQTDSKASQNEEDGFSLQEADEMLKCVVESIGRIMELVPNMSGTYIYEILEVMLTGIVGSYVETALEVAYSRAVRMDSSKPPQLELSFMKYISLSTEILSLTSASVKAVFLPLLSNTPSMKKQTIEMTNNQIRRCELLINIILEEVTRIFLNKFSTSLAKQKKKDFFPKSQELLDHDTLPAVEVVNVLNSLHTQAALYLKAKNLEAFLSKIGNGLYDLLIAHYGKFQVSSTGGIIVTKDIIGYQTAIEDWRIPALYDKFTVLRELANLFTVQPDLLDSLTKEGHLADVNKDIISNYISHREDFNHENFMGSVRLNLRQYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T427</accession>
    <name>A0A8J2T427_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methionyl-tRNA formyltransferase, mitochondrial</fullName>
            <ecNumber evidence="2">2.1.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_08944g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fmt family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.9" evidence="2"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T427"/>
    <dbReference type="OrthoDB" id="10268103at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004479">
        <property type="term" value="F:methionyl-tRNA formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd08646">
        <property type="entry name" value="FMT_core_Met-tRNA-FMT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005794">
        <property type="entry name" value="Fmt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041711">
        <property type="entry name" value="Met-tRNA-FMT_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00460">
        <property type="entry name" value="fmt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138:SF5">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="FMT C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="6">
        <location>
            <begin position="38"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Formyl transferase C-terminal" evidence="7">
        <location>
            <begin position="258"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="384" mass="43560" checksum="D5645F1833ACF36B" modified="2022-05-25" version="1">MECEVALSPVLQSYRTQKIMLGIKAAQFISKRWHSLNVLYFGSDEFSIHSLKALNDLKGGAVSNIQVVTRPSKWCGRSKSVLKEVPIVKAARSLGMPEAITCDSRKEMLELNELIHQKHFNMVMAVSFGKLIPAELLARIPYSLNIHPSLLPKYKGASPIQYALLNNDEYTGVTIQTLHPCHFDQGAIVAQSSPLNLQDLLAKGTVSQFSEDYPRRTAILMDQLGLEGGSLLKKVISDKLYLQSDVYQPPYEPSYAPKITSQMKRLDWLKDSARTALNKLETLGPLYAFKEVEVSKTKKTMDEVSLKRVIFHRFHVIRTQNWGLTPGQFTYHDTTKSLYVQCHEGALQLHDLQFEGYKIENAKQFMASLRKRCGTRLSKNLFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T456</accession>
    <name>A0A8J2T456_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_13256g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_13256g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T456"/>
    <dbReference type="OrthoDB" id="5576775at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014840">
        <property type="entry name" value="HRD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08729">
        <property type="entry name" value="HUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hpc2-related" evidence="2">
        <location>
            <begin position="564"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="383"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="525"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF88567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="629" mass="67935" checksum="632177396CEDE458" modified="2022-05-25" version="1">MEQERDQEALSTSKERSTADGDDPQHEKANIKSGNDIPQETKNATTTKDTQIKPRKRARRDDGSKATKKLKLDGKKIPNIAEELAKNRNIVRGSSPTSLTSSSCAPITSASAFTATVKGDSERGTPEPADLVLRKEVNPRRTQVKISSLLSPNGDNSSSLAAQNKSETESSPVTHTSLLMQASQSVNTPKVILPTQLPGSSQPDANDIPSEENGVLIARISSPVSVPPQSQLKRSTSSAAGEHKTPSEKAQKSNTIGAIQSRSKQTKTTGEPKKRNTKTGTKRENGSSGTKKDGSSVSSRKNSSKKDGSANGAKKDSSMTGDIKTVDATTKKDNSTTSLKKENSAESFKLENSTPGSAKASDKNERKDGKLANKPAGKKEGNENTASNYNSNNNSSNNNTNNASNKSNDAKKKITVKRESSSTAKPTKTPKKLIIAPPIKSPSLLEVIERGKTQEEPEEPALIVDIPLYSAEDNDYLDENGQVVFNFYKIVQDKFSPESADNLAGVKSAKRNLFGHITGSQLDGAAEEEDEDIEEVDEDGEDDEDEEGEEPKQNASPKKKSHPNKGKSLIGKYDTEDPFIDDTELLWEEQRAATKDGFFVYFGPLIEKGHYASLERADGTMKRGGVKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T461</accession>
    <name>A0A8J2T461_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="17">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">BN860_08328g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="function">
        <text evidence="16">This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports the calcium to the vacuole and participates in the control of the cytosolic free calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 17">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="17"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T461"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000028">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000018">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 17" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 17" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="352"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="862"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="888"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1024"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1056"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="28"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="477"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1117"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="482"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="492"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1127"/>
            <end position="1137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CDF87523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1137" mass="125517" checksum="31DBB8617A7787C9" modified="2022-05-25" version="1">MSSEVEFNLTADQLASMYEPKSLAKFGQLFDNDPKKLYTELHTDKESGIQEGTGRDTERYRRFQDNRIPERPPKTFWALAWEAFNDKTMLLLTAAAVVSFVLGLYEALTQPPEYDPDGNKIPGVDWIEGIAIMLAVVVVVLVGAVNDYEKEKQFVKLNRKKEDRQVVVYRGGEEQLISVHDLLVGDLMHLQTGEVVPADCILVEGNCEADESSVTGETDSIKVAPLGQVWKKYTKALHGESPPVNDSNSELQGDAADCMLISGSKLISGLGKAVVTAVGVHSVHGKTMMSLKTETESTPLQERLSELSDSISVFGCSAAIILFVVLFVQFLLDLRKGGRLADLPPAKRGSHFMNIFITSITVIVVAVPEGLPLAVTLALAFATTRMTKDGNLVRVLSACETMGSATAVCSDKTGTLTENRMTVVKGLLGSSQFDETDEDHEITAKSLLDHDEVAPDLKQDILANVTLNSTAFENKNYKEDQQQQQQSSSDQGHTSDNPFSSVLTRGKSRLEQLLHGQDELDTPHVDKSEEPYIGSKTETALLTMAHKEFGLGDLKDWRENHQAHFNIASIVQIVPFESSRKWGGIVVKYSQKPGYRFFVKGAAEILFRRALYWRQSDGSVEKLNDEYRQRVSDEIQGLASGALRAISIAHKDLDYEGEWPPANIRDDSEESEASPDKLIGELVDSNSENGLVLDAVVGIQDPLRHGVKNSIEQCQSAGVTVRMVTGDNITTARAIARNCSILSKSDWTDPSCAMEGPAFRKLSDEKRIEILPNLRVLARSSPEDKKILVSTLKRMGDVVASTGDGTNDAPALKMADVGFSMGIAGTEVAREASDIILMTDDFSAIVNAIKWGRCVSGSIKKFIQFQLTVNITAVVLTFVSSVTTGKSVLTAVQLLWVNLIMDTLAALALATDRPDKNIMNRKPTGRRAPLISVSSWKMIMCQSLLQLIVTFTLTFRSSEIFLGRKATGHEHQMLNACTFNTFVWLQFFTLFVTRKLDEADGISDWKKRISKENLDFFEDLSRNGYFLGVMAIIGGFQVLIMRFGGAAFSIAEQTRSMWACALLCGTLSIPIGALVRICPDEWAMKIYPRRLFNFLRFIFSFGFYKKDKKRGLHADEESLLGTDQDESSASASANEYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T489</accession>
    <name>A0A8J2T489_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_15896g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_15896g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T489"/>
    <dbReference type="OrthoDB" id="186625at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16180">
        <property type="entry name" value="EFh_PEF_Group_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051426">
        <property type="entry name" value="Peflin/Sorcin_CaBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212:SF3">
        <property type="entry name" value="GH27120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212">
        <property type="entry name" value="PEFLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="134"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="114"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="312" mass="35826" checksum="B839C3F0648641F5" modified="2022-05-25" version="1">MREGRRRLEYQKQQKHYHRHEAKKSVDTDPRRHTTSPVMSSGGYSDRNSNPSISQSQQQLPAAVRYHPHQQVPSVGCSPRSSPAGLNRVKPPPMAACSTPPLAAAARLPTSSPMCNTPNSSGSRNSYDMKEESKNIQIATQLFYNHDVKNKGRLTAEELQKLLQNDDCTHFCISAIDSLINLFGATRFGTINQSEFVSLYKRVKYWRKVYVDNDINGSYTISVSEYHNSLQELGYLIPFEVSERIFDQYAEFINHNFNGKELKFDKFVESLVWLMRLTKCFRKFDENQEGTATIHYKDFIDATLYLGRFLPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4A3</accession>
    <name>A0A8J2T4A3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosome assembly protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Elongation factor-like 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_15016g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4A3"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01681">
        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04096">
        <property type="entry name" value="eEF2_snRNP_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01885">
        <property type="entry name" value="EF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16268">
        <property type="entry name" value="EF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16261">
        <property type="entry name" value="EF2_snRNP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000006">
        <property type="entry name" value="Elongation factor like GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000746">
        <property type="entry name" value="Ribosome assembly protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056752">
        <property type="entry name" value="EFL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF3">
        <property type="entry name" value="ELONGATION FACTOR-LIKE GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25118">
        <property type="entry name" value="EFL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="17"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF88643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1084" mass="122129" checksum="F2B84D5536EF1154" modified="2022-05-25" version="1">MPRVDSKTFIRLQNDPSCVRNICIMAHVDHGKTSLSDSLLASNGIISQRMAGKIRYLDARQDEQIRGITMESSAVSLYFRVLRKQEGTDDPLVNEHLINLIDSPGHIDFSSEVSAASRLCDGAVVLLDVVEGVCSQTVTVLRQCWTEKLKPILVLNKIDRLITELQFTPQEAYVHMNKLIEQVNSVIGSFFAGERQLDDYSYRERLEENVNAEYVEKDDSDIYFDPAKNNIIFASAIDGWGFSISQMAKFYEQKLGAKRDNLQKVLWGDFYMEPKTKKIINNKGLKGRVMKPLFVSLILDNIWKIYENVTISKNLQVIEKITKTLGISLLPRDLNSRDEKNLLRKIMGQWLPVSTAVLLTVIEKLPSPLQSQRERLDTILPNFQDTKNVDTTLLNSMKNCDRGGPTSVFVSKILSIPRDELPRQTVGDPLQDAISRRMKEARDAALKAARQAEIENNPSKPDLGSIDEGVDLYQRAKDTVITPETSSLPSHRKTEINPEFDLKLESNEPYNSFVPDLTPALDLNDPLASMFEYEEDDPFSTDPSLSAAPAIISEEDPAENIFDEKDEVLIGFARIYSGTIKVGQEISVLGPKYDPSYPEEHIETAIITSLYLFMGKELLPLESCPSGNIVGIGGLAGKIIKNGTLIEKEVRGINLAGVNFQTFPIVRVAVEPQNPMEMNKLVRGLRLLEQSDSCVQTYVEDSGEQILCTAGELHLERCLKDLRERFSGIELTCSEPAIPYRETFIEASDMNMPRNPQLGRGMGELQQGPFKVKMRTFPLAKDTIKFLAENETIIQAFHSADSSDQEVFIKKWREVSRKDKLLKDALNNDDNDKIAAFGPKRVGPNIVLSERRLLGSFFKFFAPAFEFADSIINGFQLAVNEGPLAREPVEGMCVVVEDIIETTVGNMEANHDSQYQKDLTNISGRFITSTRDMIHATFLDWSPRMMWAVYSCDVQTSVDVLGKVYSVVQQRRGRIESEELKEGTPFFQIKAIIPVVEAFGFSEDIRKKTSGAAQPQLVFAGFECIDLDPFWVPTTEEELEELGDTADRENIARKHMNNVRRRKGLFVDEKVIKSGEKQRTLKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4B0</accession>
    <name>A0A8J2T4B0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b-c1 complex subunit 2, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Complex III subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Core protein II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ubiquinol-cytochrome-c reductase complex core protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_19196g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4B0"/>
    <dbReference type="OrthoDB" id="6369905at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000021">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF209">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="14">
        <location>
            <begin position="19"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="15">
        <location>
            <begin position="167"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF87994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="369" mass="40456" checksum="10E35A2369C222A9" modified="2022-05-25" version="1">MFSSSARLQIAKQAARRYTVTARDGSGKVSSLAVKVHGGSRYATKDGISHLLSRFNFHNTSGKSALRLVRESELLGGKFESSVDREYITLTATFLKEDLPFYVNALGNVLYKTSYKPHELPESVLPASQYDLAIANTCPVKRSEELLYNITYRHGLGNPVLYDGVEKVSVEDIKAFADKVYTKDNIEIVGEGVNETDLKKFVSDSLLNSLPSGSSLVSSAAPKTFVGQENRARFAGQSVAAIAIPVTKNDFAQYEVLARYLTSPLSELSPLIFKSKLDKHSTTGLFTLFVKGSCATQVSENIKKVVSELKQGKDISVAKDYASLQYSIEADSTVAPIELNFDSVKHFKLSKFNYVAVGDVSKLPYADEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4E5</accession>
    <name>A0A8J2T4E5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_14488g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_14488g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4E5"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16982">
        <property type="entry name" value="CID_Pcf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000016">
        <property type="entry name" value="mRNA cleavage factor complex component Pcf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045154">
        <property type="entry name" value="PCF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054127">
        <property type="entry name" value="Pcf11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047415">
        <property type="entry name" value="Pcf11_CID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021605">
        <property type="entry name" value="Pcf11_Clp1-ID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054128">
        <property type="entry name" value="Pfc11_Rna14/15-ID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921:SF3">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX 2 PROTEIN PCF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21936">
        <property type="entry name" value="Pcf11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11526">
        <property type="entry name" value="Pfc11_Clp1_ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21940">
        <property type="entry name" value="Pfc11_Rna14-15-ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="2">
        <location>
            <begin position="4"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="612" mass="69310" checksum="DFDDE7A9E9F2E70A" modified="2022-05-25" version="1">MDKESEIVIKDFTSILEELTLNSRPIITTLTKIAEENIPCAQYFVDSLEAKIEKCPPKQKLYAFYALDSICKNAGSPYTIYFSRNLFNLYKRTYLLVDNTTRTKLINMFKTWMVPNETTGGYSPLFERSSLEKIEQFLIKASALHQKNFQSMLPTPTVPLLMREIDKLTALTTERLKVQPQDEKLNMKLVVLSQLKQELQREKLTSAALKQLQMQLRNIFAQDQHVLQERQRHQQQEQQIQQQRQQQHQQQQLQHQQQKPMYEQSVIPLFGDGSSGSSNSSSLFGGSQSILSPLNFSELDRQNKRAKLQELYHSLDEEGLIYKPPKESIVTLSSKLGADGNGISIDQQQMRKVLPPINLLKGILADCKASFATVNVDILNTPNLQLSQQVISGNNPIVNNCLVHLLYRAKPNKCNVCGKRFGNNVEEKRLQAEHLDWHFRIHKRMKGSEGTAASNAGMGGSAAQKNIQSRNWYLHDSQWVAFKDEEIVSTNRKADESLTLANLDAQSTSTTTAAPVNNNKSPETVAVDEGILRKKYVVVPESAEDMSFQCPICKDTVTGLYDEDLGEWVWKNAMEAGGKYFHATCYYEASKNNSGSNVVQLDLEKLKHLVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4M9</accession>
    <name>A0A8J2T4M9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clathrin heavy chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_04236g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasmic vesicle membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Coated pit</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the clathrin heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2113814at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071439">
        <property type="term" value="C:clathrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000001">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000002">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000082">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.110:FF:000003">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.110">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055358">
        <property type="entry name" value="CHCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015348">
        <property type="entry name" value="Clathrin_H-chain_linker_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016025">
        <property type="entry name" value="Clathrin_H-chain_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022365">
        <property type="entry name" value="Clathrin_H-chain_propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016341">
        <property type="entry name" value="Clathrin_heavy_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292:SF1">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00637">
        <property type="entry name" value="Clathrin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09268">
        <property type="entry name" value="Clathrin-link"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13838">
        <property type="entry name" value="Clathrin_H_link"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01394">
        <property type="entry name" value="Clathrin_propel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002290">
        <property type="entry name" value="Clathrin_H_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00299">
        <property type="entry name" value="CLH"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50989">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0168">Coated pit</keyword>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clathrin heavy chain linker core motif" evidence="9">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="542"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="691"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="838"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="984"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1133"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1279"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1428"/>
            <end position="1571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1625"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1626"/>
            <end position="1648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF88185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1648" mass="186118" checksum="6D5EA709E37CB9E7" modified="2022-05-25" version="1">MSDLPIEFTELADLTSLGISPQSLDFRSTTFESDHYVTVRETQDGANSVAIVDLTNNNNVIRKNMGGDSAIMHPSQMVISVRANGTIVQIFNLETKSKLKSFTLDEPVLFWKWLNDKDLGLVTARSILISHVFDGNVSAKPQLLTLRHASLNNTQIINIVTNENEDWFAVVGILQENGRIAGKIQLYSKQRNVSQAIDGHVAIFAHILLDGNVSPVQVFVTGNRNVQTGTGELRIIEIEHDSSLPVTYQKKNVDIFFPPDAVNDFPISVQVSEKYGVVYLLTKYGFIHLYELETGINLFVNRITAESVFTATSYDHKNGISCINKKGQVLAVEIATSQIVPYILNKLSNVSLALTVASRGGLPGADNLFQKQFDSLLSQGDFQGAAKVAASSQQLRNQNTINRLKNIQAAPGAISPILLYFSTLLDKGKLNKDETIELARPVLQQDRKQLFEKWLKEDKLECSEALGDIVKPFDTTLALACYLRAGTHAKVVSCLAELQQFDKITAYCQKTGFQPNFLVLISPLIRSSPDRAAEFAISLLQDQDTASQLEIEKIADLFFSQNHVQQGTSLLLDALKGDTPNQGHLQTRVLEVNLMHAPQVADAILGNNIFSHYDKPTIASLSEKAGLYQRALENYTDIKDIKRCIVHTNALPVDWLVAYFGKLNVEQSLACLRTLMESNIQSNVQIVVQVATKFSDLIGSSVLIKLFEEYRATEGLYYYLASLVNLTEDKDVVYKYIECAAKLHQFKEIERIVRDNSVYDPEKVKNFLKNAQLENQIPLIVVCDRFNFTHELILYLYKTHNMKFIEAYVQQVNPSRTPQVVGALLDVDCDENFIKGLLQSVLGQISVQELTEEVEKRNRLKLLLPFLEQTLAQGAQEQGVYNALAKIYIDSNNAPEKFLKENDQYDTLDVGHYCEKRDPYLAYIAYDKGSNDEDLIRITNENSMYKYQARYLLKRSDPELWNSVLNAENSHRQQLIEAVISVGIPELTDPEPVSLTVQAFMSNGLKIELIELLEKIILEPSSFSDNVALQGLLLLSAIKYEPSKVSSYIEKLENYDANEIAPLCISHGLTEEAFEIYDKHEMYDKALKVLIEDIMNLDRAQTYVEKINTPSLWSQLGTAQLDALRIPSAIDSYIKAEDPSNYENVIEIAEKEGNYEELLPYLTMARKTLKEPKIDGCLILAYAGLGKVHEIENLLSGPNVANLEDVGDKLMDIQNYKAAKLCYASVSNYSKLASTLVYLDDYQAAVDTARKASNIRVWKLVNDACIDKKEFRLAQICGLNLIVHAEELNELVEKYESNGYFDEVISLFEAGLGLERAHMGMFTELAILYSKYEPSKTYEHLKLFWSRINIPKVIRAVEEAHLWNELVFLFAHYDEWDNAALTMIEKSAKNFDHSYFKEVIVKVSNLEIYYKAINFYVLEHPSLLVDLLAALTPRLDIPRTVKLFAKSDNLPLIKPFLVNVLPKNNSVVNQACHDLMIEEEDYKALQDAVDSYDKFDQLALAARLESHELIFFKKIAALLYRRNKKWSKSLDILKEEKLWKDAIETAAISGDSKVVQDLLTYFVETENREAFVALLYTSYHLVEYDFVLEISWLHNLGDLSKPYEISVKKEQSDAIKRLAEEMAEKTSLSENPTDGQTLMLTNGAPSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4N1</accession>
    <name>A0A8J2T4N1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="16 17">Ketol-acid reductoisomerase, mitochondrial</fullName>
            <ecNumber evidence="5 17">1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15 17">Acetohydroxy-acid reductoisomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14 17">Alpha-keto-beta-hydroxylacyl reductoisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">BN860_15610g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13493"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49256"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="17">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17 18">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.86" evidence="5 17"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4N1"/>
    <dbReference type="OrthoDB" id="10255643at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00056"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000003">
        <property type="entry name" value="Ketol-acid reductoisomerase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000005">
        <property type="entry name" value="Ketol-acid reductoisomerase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000013">
        <property type="entry name" value="Ketol-acid reductoisomerase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000167">
        <property type="entry name" value="Ketol-acid reductoisomerase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013023">
        <property type="entry name" value="KARI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000506">
        <property type="entry name" value="KARI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013116">
        <property type="entry name" value="KARI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016207">
        <property type="entry name" value="KetolA_reductoisomerase_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00465">
        <property type="entry name" value="ilvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371:SF1">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01450">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07991">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000119">
        <property type="entry name" value="Ilv5_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51851">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51850">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17 18" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="13 17 18" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8 17 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 17 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 17" id="KW-0521">NADP</keyword>
    <keyword evidence="11 17 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="KARI N-terminal Rossmann" evidence="19">
        <location>
            <begin position="63"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="KARI C-terminal knotted" evidence="20">
        <location>
            <begin position="253"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00072637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CDF87838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="401" mass="44795" checksum="543D37802279E0C4" modified="2022-05-25" version="1">MFKNSTRTASRFICNSRVITAKRTFALASRAAAVNAVRPATRQFIKPLVATRGLKQINFGGTVEKVYGRSDWPREKLLDYFKNDTFALIGYGSQGYGQGLNLRDNGLNVILGVRKDGASWKAAIEDGWVPGETLFDVNEAVKKGSIVMNLLSDAAQSETWPTIKPLLTEGKTLYFSHGFSPVFYDLTHVEPPSNIDTILVAPKGSGRTVRSLFKEGRGINSSYAVWKDVTGKADEKARAIAIAIGSGYVYQTTFEKEVNSDLYGERGCLMGGIHGMFLAQYEVLRENGHSPSEAFNETVEEATQSLYPLIGKYGMDYMYDACSTTARRGALDWYPIFKDALKPVFEDLYESVKNGSETKRSLEFNSQPDYRAKLESELDVIRNMEIWKVGKEVRKLRPENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4V8</accession>
    <name>A0A8J2T4V8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA (guanine(37)-N1)-methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA methyltransferase 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TRM5</name>
        <name evidence="12" type="ORF">BN860_18140g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="10">Predominantly in the mitochondria and in the nucleus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRM5 / TYW2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="10"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4V8"/>
    <dbReference type="OrthoDB" id="408788at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070901">
        <property type="term" value="P:mitochondrial tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.110:FF:000001">
        <property type="entry name" value="tRNA (guanine(37)-N1)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03152">
        <property type="entry name" value="TRM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025792">
        <property type="entry name" value="tRNA_Gua_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="11">
        <location>
            <begin position="171"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="300"/>
            <end position="301"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="328"/>
            <end position="329"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF87950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="484" mass="56010" checksum="3949E49D5DB87695" modified="2022-05-25" version="1">MRIRYIFNQSTRRQIMNSTLTTFKFQPPVNRDMRTLDRSFFVVKVPLCVVRFPDPKNISLFSKEFKDYILRVPRIPHVVKLSTLSEVTPQDGKTLACDNKIVSKGVLLNDNIRTVQEAYKELPQEAMDFLKNSEAELLPYEKVLDYNFWKAEEILRAVLPLDLLDEIPTGFTVTGHIAHLNLREEFKPYDSLIGQVILDKNNKIETVVDKVSSIATKFRTFPMKVIAGRDDSLVVEQRESDCVFRFDFSKVYWNSRLHTEHDRLVSKYFQPGQVVCDVFAGVGPFAIPAGKKHVFVLANDLNPESFKYLQENIKLNKVDPFVEPFDLDGAQFIQQSPQLLEKWANRVGKVSIQLNKRKRQKKEDQHGAPSIKEFSIPLEIHHYVMNLPDSSITFLHYFKGLFNGSCIKNLPWIHIHCFEKHGTDEEPTMDELHERVHKRILRELSVDDSTLPLNQISIHLVRKVSPTKPMFCASFQLPKSVARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T500</accession>
    <name>A0A8J2T500_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_05512g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_05512g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="336088at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048189">
        <property type="term" value="C:Lid2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036205">
        <property type="term" value="P:histone catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04710">
        <property type="entry name" value="BAH_fungalPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15667">
        <property type="entry name" value="ePHD_Snt2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029617">
        <property type="entry name" value="Snt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47672">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SNT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47672:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SNT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BAH" evidence="7">
        <location>
            <begin position="122"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="315"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="1081"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="620"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="635"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF88243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1476" mass="170104" checksum="73D93B56D3918343" modified="2022-05-25" version="1">MTGDQPSILSRRRATTKAVNYNEKDADAELVKRIEQLEEYRSANQDNKPKRGRGGGIATNCSANSTDKLRYQKYLSDKSIGWNFIPSLPSFFRKASRFSIVLDLEGALLDVREQCIYNQGSVLMKANDTIYMISEPPGEPYYIGRVVEFICKPEFRSIITKVKLDTERYPVKFFEVRMNWFYRPRDIQNRVNNLDPRLLYASLHQDICPIESYRGKCSVFHEDDLNDSMLNSTERLTKPNNFYFNHLFDRYTRKYYKVVSTKNLLELQADSPFLRTLSKRFRYIYFEENYRLDEVIQKYVLEEGNATSREDVLWNERCSVCREWCTKPLSLKCDECTQGVHLYCMDPPLDRKPNKGVIWLCFGCLKRQEGTVQALKEAEEDDLGERSFFDSSRKDLNDIAMRTLNKSGPLNQKCCWMQYIGWNMINHLSDLMHEGVFFPYPMKYSRIGCKYQWSGCLNDDDIFPQPYSTEGTGAVIERGTERTSDLLWKMNNAKSCPGEVDSYLERCKAEIPPKLDMLPESCNFLDMAVTMLMNNDFDAKLAFQQCCNQASRESLQEPTFTAEEVMQFERAISEYGSELLPVSKCVKTQPMSMIVRYYYYWKKTPSGRSIWGCYKGRKKNAGKKASECPPSSTNPEERKSGRERKPTKLALESQIPNSQSDLKHVDDSSFETEKLFTLKKYFHCMFCEIDYSPMWYKVTGGSDDDYIKTRMQTGVNERTETSGNNLLSNAAAKSKNKERENENEKVDALCMRCARLWRRYGVKWEHPLKVLKKLNGPSVASLHATLELIFSESSVPALRLSPQQARNKLIEWELVQDAELISSQREEMMKDPERLAKLKKNTMASRPQLFKMVKRPFDRTAWTSGHMIKELTEYMENHRSKNQAPKNISAKDNNRIGKICKAAHITKGYGTIDKKRKESEKNKASLVHLKNIGAVKLAINRDIHFKLSNKDEDIGKITIDSDGEYFQIDDKIYAELMCHMRTLKRVRSMDPSSEVFTFKRARLATFDDINNFSNRYQRSKKPSLGVCENTSDTTKLLGMYHKLNPFYVNWYNMVGTESMTSLRSTPVKHQEFEEKKLTELVACCCVCGEKFSDSVDEEIICQSCGLRVHHYCYGVSLPYDLPMKMRLKDFKWLCDPCSNNLHPIVSTDYRCDLCQFRKCPEIIAKGQQEPLKLEALKCTTVGSWVHVICAVYNVSIKFVSSKFLQPILNTKFVLAEGLCQTCSICKLQGGGIIKCEVCCTRFHASCAYCTPGFELRIKKIALTDLQKNEPGVLLSDGKLYVLNPVAFCREHQQELSVSDDLFFPLNWKFASQSETLLQLFCKHYKSCNQTSTVYARYIEQQSALRGGLRPDTEERTRDIVKKGAMIKIKTSFIPEANTCSRCGTHKAIFWYGDICHVCHVSQKLHQEKSTDISESQIAEHIQIVPRISEAVSATLLEGIVPLKTEVKTEKSNKTTKTTDAKDLIQKTRWIKENPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T536</accession>
    <name>A0A8J2T536_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_15258g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_15258g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T536"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="3">
        <location>
            <begin position="5"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="182"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="537"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="978"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="204"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="232"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="285"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="334"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="360"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="552"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="574"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="595"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="613"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="635"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="646"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="720"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="749"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="785"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="835"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="873"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1051"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1075"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1114"/>
            <end position="1125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF88653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1154" mass="122702" checksum="50972123F7763647" modified="2022-05-25" version="1">MSSIDYSFRWPAGPEHVVVTGEFDDWKGTVSLEKSPTTGEFEVTLPISVAADKDKIFFKFIVDGQWVTSGFYDIGNDPEGIENNYITKRELLGLGDTPSAGSDAANIGADPARATQQTTIREQIPTSEVVTKSSTVTPHNDNVPDVVVARSSKSLNVRPSAPIVQRHAASINVAPSEGIMGAGSAVGSSSASSASPDTTSYSGKKVRVKRRIKRNKKTGESIVVSEVRTPLSDDEGLGNTTEEEFSPAAVKNPATHVPGSVEDTPTRNSTKNNPVLVVSAVAPGTDNSLTSSPSSRKSHNSAAVKSSPKSTSKKERPTTGLVGSSSGFPRANSPCRSKSGAPSTPSREERPTSAVVEPGSGSSSRNDQLSRTASSKRHSHYVDAIGVGASPLVASGAGSGAADFGRHSSEVDHPVHILPIENEADKDNFKSIAGEPGPYLPLNQEQLEAFPQVNDDIDPKAFNERLSKQFKAEHDKNGIKDGKAEEQKITKKAKEKVTDHGLQTQKALNDFDKEANADKLEHINKADTIDKVKGAKSALEGTATRHKSHKQSKNEIKKQAQKDKNKSIIPSKTATDERKIEADAQSDKVIGGNDNVGNATSQTQPVKDVIASEIRKDESIRKVDGDIVHKGAELKNTTKGVTPDTSSHEFVESKGKTPTQDSKRDSKKISKKDVKKDSKKDHNNDTKKDSKKDLKHAPKDTSVVIGKEEEITQETFPDLQGKEPTLHEKVKKTDTVRQKSTSVKKTKDKKKELPNTPKKSESTKKTDSPKKKETKGAKSHKKHDSLHTLDPRVHKDSENDGEAFAAHKSHGLEGSDGEDDLYVDAVTGGTRHSVGGTTSTAHTTEITGNSDAPNVGVTSRSPDKGSSKKNKVGSDSTRNTIPGTESTVKSSKHADHEFKRKSVGSEPKHVVASAEGASENVVSTNKDDLSAVKSKDIGMGKKRISNVTGDGEIVSEKYRNSIIGADNDETPQDLRAVKSGKSDIKHGKIAGVPSTEEIANAPEVNSTISGQPGPDTTHVKGAKTETAPSVVSDTDAADIAKSNEDDTNKSKTSKSRPGKSKTAKNGHAKHKTSKTGKGSTSKTGANKTTQGKDGGAAVKTAASKVENSPKKAAATTSSRKPPAQNTAAAAAKAADSTDKKKKLGLWARVKRILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T570</accession>
    <name>A0A8J2T570_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nuclear mRNA export factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_12024g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the SAC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="264795at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.990:FF:000008">
        <property type="entry name" value="Nuclear mRNA export protein SAC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017173">
        <property type="entry name" value="Sac3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045107">
        <property type="entry name" value="SAC3/GANP/THP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005062">
        <property type="entry name" value="SAC3/GANP/THP3_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024293">
        <property type="entry name" value="SAC3_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436">
        <property type="entry name" value="80 KDA MCM3-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436:SF3">
        <property type="entry name" value="GERMINAL-CENTER ASSOCIATED NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12209">
        <property type="entry name" value="SAC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03399">
        <property type="entry name" value="SAC3_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037320">
        <property type="entry name" value="mRNA_export_factor_Sac3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="7">
        <location>
            <begin position="329"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="22"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="628"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1245"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="630"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF89221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1302" mass="149162" checksum="738E79688F92AE82" modified="2022-05-25" version="1">MNIPFGATASASGFNFFDGQKTVHHSSGNKSNEFTKIGDKKTAGDSGQLNRSGKHQEKHTFRHSDRGRNAGKDTDLRKSKLAHISASENSRIGSLVASPQQFGFHKMSHKSRPVPRFLINQQPQLKRKEFVQDPWDAANQQKMLQLEANIDDFSELYETLRKMRDVERKMMESKHLVDKADSAKDLTEAINFQGTCQDMCPVFERARRNVEYTVYSYEKNHHEDKKAARDKALKVFSRPAAAAAPPLPSDVRPPHILVKTLDYIVDNLLTTLPQSEGFLWDRMRSIRQDFTYQNYSGPEAIDCNERIVRIHLLIVHIMAKRKAEYSLQQELEQLHKSLITLSEIYDEVRASGGSCPNEAEFRAYALLSKIRDPEYDKSIEKLPSSIFQNDLVQQALCFRRLVSNSSYVNRGVIRTENCLNFYQRFFQLIATQRVPFLMCSFLELYVNEVRFYAFKALSYSLNKKHKPIPVPYFMENFLFNDEEELFDFCKYYSIEVSPDGVELKTLPNNSHKLPERQPMRQTYLRCVDNMLQDSTFSSIVNSGKLNVDTIPAPGSNSLTPNNVSEVVEGLEHGSEQEADAGEMEMEMEEDRVIEMGKDTQQKNITDNQIWQRDVMKPTFPYHSSHIPPVTSLSRSTEPPSSQTALQFEPTNKSASTQTAGFQVQQLPLKNDDINLPTQQMPLYVTSNQINPENEKLKEQPLIDSSKAKMEPKKIIEKQKDLIKEQQELEKREAASALTNQVVEEVVHKQAVAAVKEALQKGKERQVKIEELADVLYHAFIHEKLYLMYLKSKAQTHYANKLRSKLFSKWRDHYLSQKRRKQDEQKKKEDIQRVQKQLGVPTFKNSFSTTPHNDDSGNNSFFFSNTRKQDITFSPVNNEVNKFDVQANKETEIWVPFPIRELYCKKINDKFSFLDSPNVADIFIYGNHWDSLCGNWLLKKFGLTCSDKPHNISNDYLLMNLRCIGKYYNPSMFTNVQLLVFNTGVTDPNIFDLELKLQQDGEELIKLITGISLNTNICFSLLVVYWESAETPLAESVVSKCLKLNRICKGFSSVLEQIEVVNINSHMPHKVLEKGLIEIANNYEPKLTDRGRYNRNLRERRSTQVDSHLLTTSAIDEKMRRMHEYEEKKLHRMQDERNTYAHLQSHIAASPKLKKRKLPVLLSKKREDRFETPQTRRTRSSSSPAMFSHLAVKLGRSARLGNFNLAPATPSHSTNVPIAITDSHETPPFPAEQLELQEPALFQTPINSTPNAIRQPAEDSEASHSISGDATTPPVAKGIPSNIMELKNLIESVRSKVNGSMIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T575</accession>
    <name>A0A8J2T575_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BRO domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_06436g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T575"/>
    <dbReference type="OrthoDB" id="2141925at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09242">
        <property type="entry name" value="BRO1_ScBro1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09237">
        <property type="entry name" value="V_ScBro1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.560">
        <property type="entry name" value="alix/aip1 in complex with the ypdl late domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.50">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025304">
        <property type="entry name" value="ALIX_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF30">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13949">
        <property type="entry name" value="ALIX_LYPXL_bnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="8">
        <location>
            <begin position="4"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="788"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="548"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="826" mass="94610" checksum="936D29664503F580" modified="2022-05-25" version="1">MRAYLITLKLKDTEAVDWKKGLISYLKRSYGSAQWSQFYNDKLAGELNHLRNNANGELALEALLEQNLLYYAYLEQLHLRIGNNSPQLRLNFTWYEAEYNSSLKAIKYSQHNIILEKSCVLYNIGATLTELAKDKLNKDYKASLNYMLNAVAIFEYLSENFLNSPSLDVQAENTKFLAELCHAQAQELFLLKLINGANPMKQASLISKLSHSCACLYETCHEFSEDEEEEIRDYGDVKWHPIISCKVYFYKALTAYYHGLALEQQDRHGEAIAFLELSADYLESALPYKTYLKEFIDFSAFKECIEDKKRQLTKDNDFIYHDNIPAAVQLEVVRSMDAVKSTAFPKQLESYMDAVANKCDNLYKGIVPMEIYEKESIYSEEKARFLRSEMDVVETADWEYTSFMEFTNLPKLIQDLERKYQGGTFTDGDSMADDMKEQLSSWSRTVRDSKFNDIDSQINDVNNKRRKIMDILSTVPSDQKESTVKIKAALLEASESDNKLFSLVKPYISEIKLLNQNALLWQTWNKYNSFSINEPSLLDIDDSKTDAVLAKLEKVKQLAEDLRLLKEERTRSLAELKEEANNDDITKKILINRGSTDSELKEMFEQELAKFKPFSARIEATIFKQSTTINEIKLELDNIFKLSGIKDGTGQEQARLEERKRFADRLRKASCNFSIFCTDLPKGLAFYDSLLRISKELANSAASKPAVQPLTASFGGENVTPRQLSLDSQFQNLNLSSSTPAPLKPSSNYGGNFLRPTVETTPVFGNNNNSLSAPPVPPKEFAPMRKEFRSSQQECETEERELQQNPTSFYNRPSVFDENLYSKFSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T592</accession>
    <name>A0A8J2T592_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BN860_12134g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_12134g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Telomere</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T592"/>
    <dbReference type="OrthoDB" id="77828at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990879">
        <property type="term" value="C:CST complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016233">
        <property type="term" value="P:telomere capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.230">
        <property type="entry name" value="Stn1, C-terminal wHTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1080">
        <property type="entry name" value="Stn1, N-terminal wHTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024263">
        <property type="entry name" value="Stn1_C_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038240">
        <property type="entry name" value="Stn1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018856">
        <property type="entry name" value="Stn1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10451">
        <property type="entry name" value="Stn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12659">
        <property type="entry name" value="Stn1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="CST complex subunit Stn1 N-terminal" evidence="4">
        <location>
            <begin position="13"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Stn1 C-terminal fungi" evidence="5">
        <location>
            <begin position="255"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF88518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="415" mass="47804" checksum="427352AB6566A725" modified="2022-05-25" version="1">MSYDHVIHQEGEIQFYVPCLFIHAIYQGVTVPLLVRDVLQLVEKSKYVCLNYYQAQIWILFWGNHPLEKISVLGCVVGCRCKFIGDEDYLLFRLDDCSGGFHFLNCKCSKELALSCGMPWGSLSGRRLKVFGVFKLQWCELDVEYFEFCDNLQLEIDHWTEAIKYRAQLMLPWSFDDPSASGKKQTGPDTHDYVQQLHKAALMDEILIATPSNSLAPDDAYNLIQGRERQDSGTFGARERSVIVSCSLTEAKSNLLRVLISRDNHKTSTIELYQDLSDLLDQVAALKFQQQNLVSHVKPWEQLRTEVFNDMLGKLEQAGLVYFPNVHVLDWISLKNLHNYSVERTWTLLKLQCNVGVLDHDYIRKKLSKPELTGKAILDVFKETLRAICEAYPNFLASWWIDTDKNSSVIHFVYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5H5</accession>
    <name>A0A8J2T5H5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BN860_15280g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_15280g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5H5"/>
    <dbReference type="OrthoDB" id="1875751at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12397">
        <property type="entry name" value="RRM2_Nop13p_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000863">
        <property type="entry name" value="Nucleolar protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034226">
        <property type="entry name" value="Nop13/Rnp24_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF95">
        <property type="entry name" value="NUCLEOLAR PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="94"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="207"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="279"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="335"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="279"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="348"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF88654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="373" mass="42461" checksum="0408B79ADCFF5757" modified="2022-05-25" version="1">MVEEKRNEKEEQVQKALQDTTRKRKAEDEIEIDLSKSVPLSKKQKRLLRRGKVTLEELNAKFNLDPASVEEYKRDSNKKDDEPEDELKKKEKRIGVWIGNLSFDTTKEDLVRFFVAKTKNLENDEQQISEDAIVRVHMPLAQNDGKKIKNKGFCYVDFKTREHLESAVALSELQLNGRNLLIKDSDNYEGRPDKNDLVSSSKNPPSRILFVGNLSFDTSDDLLRKHFQHCGEIIKIRMATFQDSGKCKGFAFVDFKDESGATNALRDKNCRKIAGRPLRMEYGEDRSKRQVKRKSHAPSKQASYDLPSSNAKPFTGTSAGDADFRKVRKLGNNFPAKKYLDSNQRQKSSIALASAQRASAAIVPSQGKKVKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5H8</accession>
    <name>A0A8J2T5H8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD repeat-containing protein JIP5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">WD repeat-containing protein jip5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_14510g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat WDR55 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5H8"/>
    <dbReference type="OrthoDB" id="2288928at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19924">
        <property type="entry name" value="UTP15 U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 15 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19924:SF31">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN JIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="365"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="392"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="415"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="447"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="458"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="470" mass="52614" checksum="F76B63B039BECF4F" modified="2022-05-25" version="1">MGRHKKKASHSLDSEVLPLLEVCYTEPLFELVCHPSRPLIYSGLASGYLYCHKYDREVLKRVRSERKQEAGGEKVKPWVKVNSNVDSEGIRVIWKTKRHQGSVRCLGIDAEGEYVYSVGADNILKKADAETGKVVKKISLKEHDAKFTKLIKSTTHPYLLLGDEVGNVLMLDSETLKLKNQVKEIHGGDAINDIFQFAKRSLHKYVSLGQTTLAYWDARDSNEGQTNDTPEDKRKVLVSDDQEDEVLCGTFVDPEEGDTLVCGMGEGILTVWKPKKNDLEDQVNRIKVAKDESIDAVVPTLQDDQCVWCGVSNGNLYKVDTKRGQVVEIRQHSEADEVGFLDLDYDYGLVSGGMESVLIWDNDASTRGDEKHSGHEDETDNREGTYVDRPSAISSDGYSSNEDSDISGEGASGLSREELVKELDRDIEEGENKNNGVEKHQGDKRKPKEKHLRSAKRMRSDHGIAKFDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5J2</accession>
    <name>A0A8J2T5J2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Deoxyhypusine hydroxylase</fullName>
            <shortName evidence="9">DOHH</shortName>
            <ecNumber evidence="9">1.14.99.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Deoxyhypusine dioxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Deoxyhypusine monooxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LIA1</name>
        <name evidence="10" type="ORF">BN860_16006g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[eIF5A protein]-deoxyhypusine + AH2 + O2 = [eIF5A protein]-hypusine + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:14101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="Rhea" id="RHEA-COMP:12592"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="ChEBI" id="CHEBI:91175"/>
            <dbReference type="EC" id="1.14.99.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="9">Binds 2 Fe(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the deoxyhypusine hydroxylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.29" evidence="9"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5J2"/>
    <dbReference type="OrthoDB" id="421002at2759"/>
    <dbReference type="UniPathway" id="UPA00354"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019135">
        <property type="term" value="F:deoxyhypusine monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000099">
        <property type="entry name" value="Deoxyhypusine hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03101">
        <property type="entry name" value="Deoxyhypusine_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027517">
        <property type="entry name" value="Deoxyhypusine_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697:SF5">
        <property type="entry name" value="DEOXYHYPUSINE HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697">
        <property type="entry name" value="PBS LYASE HEAT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF88684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="322" mass="36466" checksum="E95370D5590B2D15" modified="2022-05-25" version="1">MSTDFEKRLEERVDDCSLEQLRDILINKSGKTQLTNRFRALFNLKCAAEEFETNPQTAKKATSYIAEAFGDDSELLKHEVAYVLGQTKNFECVPYLRECVLDLNQQCMVRHEAAEALGALGDKNSLDVLEQVRKEDPSLAVRETCELAIDRIKWQHSKTGSKEALQPSLYSSIDPAPPLSLEKEYQVEELQHLLNDTKKPLFERYRAMFRLRDLGTDEAALALATGFDDQSALFKHEIAYVFGQMGNPCIVPKLIEVLGRREEEPMVRHEAAEALGSIATDDVLPVLKSYLNDDVDVVRESAHVALDMYEYENSNELEYAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5L6</accession>
    <name>A0A8J2T5L6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_11320g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_11320g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5L6"/>
    <dbReference type="OrthoDB" id="5325112at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032914">
        <property type="entry name" value="Vam6/VPS39/TRAP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894">
        <property type="entry name" value="CNH DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894:SF28">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CNH" evidence="2">
        <location>
            <begin position="111"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="973" mass="112326" checksum="75CDD0B741E0AA25" modified="2022-05-25" version="1">MSDEEQLSSPLHPKRPDEAKEPKETEQPAEEHDREESKQVEEPNELPSSIPLSQQNQLEQSSETVDQGKENNSEVSPSSQGNESQESNENELEMSKGPFEKHELIRFLPSNLEYTCFEAYDENVYLGTKTGDLLHYFEIERHNYMLVSQTKLDDELDHPITKIILLPHIERALVLCNSILVVFLLPEFAPAPNTPKFKGVADVTIRNYSSQFKCYKFYAMKEDSIKLLKISSHGINVAQTFDYKLVKKACSYHYTLMTAKLNNYEIINLRDSTVTPLFQISESEAPLKPIITKFNEGEFLVTTGGGSETDSSIVFVVNSDGEISHGTMVLDEYPEEVIVEYPYVLITFGSNRLEVYKVSPNSEPQVVQKMVLKNSPLGLCRTSKVFNNFERKTMRERIIDKLRLVPLLGINHSFRIDSETAYIDQIFEEKSSLAVYGKFGIDLLIKNSPVLEFDQYGEPEIDKLEEYLIETSKTTLSHLEQSERTFLETLLLLLVMLHCSIINEEYVKKWCSMADEIDIKLLFYLLDLSVHGDIWVCNGLVKFIERLKSLNLINKCSDIVKLLRFMKNELRASKIKNSTKNYSNLVLMIDVNILKVQLQGNDNDILVEDFEEHSLEEITAIVEERSHEHTELLLKIYQRRGMLQEIINLLKKKKDIDRIFEFLTNNAKNLPPTYKPEDLTEDLMFAIKEASQLNKSLVKEALKILSLAQVEPSELLNKTGNNTRIKVFIIEEVGARSPDDKEFLVNFYMAKLQETLQDGNIWDLFAAFAAEYTKDMNYTKCSIAEFLQIKLTHNKQCQSFVEFYQNIRDLCLHDQEDRLLRSVVSQVKSFDCNHMLTLLFLPEGQVREEFLTAHQAFEAYLSFNDFLGIEKYVTETNILEVLKHYNELPRGIDSLKLTKLLLKRNMHCIRGEGTLIAILTLLPCEYSFQPFFDVVYDILKRLDDEKRQLELQKAILKDENATFKTIYTTVSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5M9</accession>
    <name>A0A8J2T5M9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_11100g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_11100g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5M9"/>
    <dbReference type="OrthoDB" id="4207519at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="520"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="319"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF88474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="608" mass="67990" checksum="F3BB9747BBF5E298" modified="2022-05-25" version="1">MEAMDSPFGPQRDHHTAHATTADPSLSAAAAAVASVQDAWAENFHEPLPTNKEDRIKQALEFMRSEGQAHAADKNMKKHSIRQIAMFFQVPKSTLYDRLKHTAAHANPNGGPENTRMSMSGPTYSVSSRAHSQQMKLSPERELTLLHQLHDLCHALGNTLNLTHIRDFIISLVQGVTLGKKWVHNFNRRHDDAVVYGTHSSQYNVKMMNSKSCRSNYEYLWKCFVPLLQERISQLPADEPFFYITRTCIHQASMSSIFTCFKIVPGDLTMTLVCPPRVVLFPDYFGRIYLQLRDLKRSEDRHGYGVAAVGDEADEVERSSASSSSASSAGSRAVDKNEKDHGVDHNKINNQEKLQKEFKLRKLNQVFHRLYTDCCIHTGQPLNGGEVTIPNGEEPLTEQASKEQVPLVIFEGFEDRYNWDPLICRSLVVLGKFLAFPWNHQLFQKMIAAELQAVVDNVAQSASITATQLSSMELDLDQVEINLDPFTRSLRHLLQSPTETIPNLNHNGVLVNDLFGSALPQSPPHTNSNTQSLAHTSANSSTPCEHHQQLENSTSANIFEDTTLAQLQEVVGMIEHNESQLYGDLADGSSSKAALMEIFKKIKGIVPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5P3</accession>
    <name>A0A8J2T5P3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA mismatch repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_11650g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Component of the post-replicative DNA mismatch repair system (MMR).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5P3"/>
    <dbReference type="OrthoDB" id="10252754at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000019">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.10:FF:000002">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000771">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003810">
        <property type="entry name" value="PRK05399.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF148">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="12">
        <location>
            <begin position="1010"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="7"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="167"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR037677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF88498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1192" mass="134716" checksum="2CACD23230100D6F" modified="2022-05-25" version="1">MVASTPKGPSSAQKKMKQSTLLSFFSKQQSTPSPTAKKSNPLKDIKTTPTQISKSPEEKKHKNRLLFVGMEDDESFVTANDSVTVYDEMCEETQNFAKEPEDSSKETSKVGDDDENDMPFKRRRRNVSYTEDEDDEDGTQSSKRSKYLSDDEDAYVPKHQSDAASDSLDDGDDVDDDEILSLADRPKKKQNKELMKTKSSSLGRTPAKKPNSRTSSPPKHSAFNKQNEERYHWLVDERDAQRRPKDDPEYDQRTLYIPSSAWAKFTPFEKQYWEIKAAMWDCIVFFKKGKFFELYEKDAALANHLFDWKIAGGGRANMQLAGIPEMSFDYWAAQFIQYGYKVAKVDQRESMLAKEIREGSKGIVKRELQCVLTSGTLTDGNMLQSDLATYCLAVREDVGDLYNVENNLDHAESAPQRIFGVAFIDTSTGELELMEFGDDNECTKLDTIMSQLNPKEIIMEKNNLSNLAHKVVKFNAAPNAIFNYVKSLEEFYDFEKTFDELVSKENNYFPDMDRWPPVLKKYYEKGKKVGFSAFGGLLHYLQWLKLDKNLISIGNIKEYNPIKSQNSLVLDGVTLQNLEIFSNSFDGSDKGTLFKLFNKAITPMGKRMMKKWLMHPLLHKSDIDGRLDTIDCLLSDTALRDLLESAFSKLPDLERLVARTHSGNLNVANFDKVIKGFETIAALIRELSDYTLKGSVSNYVASIPGSLFEKIDNWANAFDRTKAVHEGILIPHRGVDPDFDKSLDRISDLENVLDDWLLKYRKQFKCSNVQYKDSGKEIYLIEVPISATKYVPSSWMQMGANKNFKRYYSDEVKQLARSVAEARELHKSLEEDLKNRLCRKFDTDYEACWLPTIHAVSNIDCLLALVRTSESLGFPSCRPELIENDETERGLSQNGFLKFTSLRHPCFNLGTMNHKEFIPNDVKLGKDFPQIGLLTGANAAGKSTILRMTCIAVIMAQMGCYVPCESAVMTPMDKIMTRLGANDNIMQGKSTFFVELSETKKILDLATNRSLLVVDELGRGGSSSDGFAIAEAVLHHVATHTQSLGFFATHYATLGLSFKYHSMVRPMRMSILVDEETRNVTFLYKLEDGQSEGSFGMHVASMCGIPKQVVESARNAADTLEHTSKMIKQRKLATEEVQGLVPLGLQSDFARMVYGNGLNNSKLGTGENAMIYDSNVKRNVLDIIFGIVDKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5X9</accession>
    <name>A0A8J2T5X9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GPN-loop GTPase</fullName>
            <ecNumber evidence="7">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_16050g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Binds to RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="7"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5X9"/>
    <dbReference type="OrthoDB" id="243313at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17870">
        <property type="entry name" value="GPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000579">
        <property type="entry name" value="GPN-loop GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030230">
        <property type="entry name" value="Gpn1/Npa3/XAB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF8">
        <property type="entry name" value="GPN-LOOP GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="282"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="282"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="307"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="384" mass="42995" checksum="53EDB035B252BADE" modified="2022-05-25" version="1">MAVKTVICIGMAGSGKTTFVQRLNSHMRTKNSPPYVINLDPAVLKVPYGANIDIRDSIKYKKVMENYNLGPNGAIVTSLNLFSTKIDQVIKLLENKRDKFEHCIVDTPGQIECFVWSASGAIITESFASTFPTVIAYIVDTPRNASPTTFMSNMLYACSILYKTKLPMILVFNKTDVCKADFAKEWMSDFEAFQRALRDDQELNGNSGLGSGYMSSLINSMSLMLEEFYSQLDMVGVSSFTGEGFDDFLEVVNNGKVQEYDQYYKAERDRVLKLKEEKEKQRKEKSLDGLMKDLGISDGGKKKGEADADVVSDVEEGENDGLVLPDEFEDTEREYTFSASDRTQGEINDQSAPDLQSAYQEAADRLRKDVSSETAENIAKYVRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T607</accession>
    <name>A0A8J2T607_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S03-07580g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_07580g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T607"/>
    <dbReference type="OrthoDB" id="4085524at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="763"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="918"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1052"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="323"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="346"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="462"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="474"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="503"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="526"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="563"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="613"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="637"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="770"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="783"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="815"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="859"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="919"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="999"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1062"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1095"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1109"/>
            <end position="1118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF89031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1209" mass="133640" checksum="45EE48895E21E1C4" modified="2022-05-25" version="1">MITRQRLSRGYERPADAEAVAAASAIGRTLSMNGKGLGENHFSSRNGSFLGDSRRTISMTGARHNSSRTPSMTRTRSIVRSESIQGPMRPSARNSMRTSSFMSHDGEIYEDAQSAFNEFGGPQAKGVIHRSPSMQQRPKTVRKYVPSYKGLIAVEVPVEEAAPRPQQRTRTITRPSSSRNGSLTRSSTQGNISLSKNQSRTSSITNGRNTGSRTISFGGSRTASFTGDRPSQLTGKTGMHSQPKRYSSLTNASLANVPRREHFMSTTLEEEEEEVKPLVIKKDDKQRRQIRIVRRVIPSNSGSVKTIKTIKTIRRVPADVPTAATSSMKKSSSTGGSVAVKHSTPKVRVSAAVKPVVKSAQPPTKISYPAEQVSSPARENSKNNTQTPVDVPELENDSSSLRAAEEEEYATPPLTTGQSPSFSPPEATISPNFESVEVAEPQELLREEELEEELENELEEPLTDKALEGRQIEEDSQLDEVKEDQEQEFDQTADQTWDNELEQEIKQSLENEPLHHSLAQQSPQEDTIEEEPEKEEQEERNEYQGNDEERSEDEQVVQPEGSAFHEESEPSRLKSQEPFEALHLEQQQLSEALHLESHEPSEALYLKSQLPSKQEDSENLSLKDEDSQSLHLDDGDTQQIVSQEQKEFQTLPSEQESQSSSLDQRKSQDLPAEQTDAPVLSQERFSEDQLDDILKAPVPAIQVDDQSSEIQSPARASPVREAPQARLRNYLLDADSYIEKSGSESASIGDQNLTDGIAPWKMISRDSQETDASGANISNNTAQEDRLAREVRRKLDDMGQHSNSNSDENSDDLFVDSREDILEEIDSHGKEQPYYNPYLSNQSGVYPSRGLVKQSSPIKSALKKTTTSASNTSSIYSDASPANQAYLSLTTAENTRLNAQLTSNDSIPRKASYKHLNRSRSLLNSNKGNHSLSPPPREAARPKRHSHHVGIRSPGAQQQPNKRLPPTRVKPTKVTANRMSRNEPAKAAAKPQVHNPHLYPREPPPRRSSFEKIRNKDNNVGMAKMSMRNGGSVEDNYQQSTLDSHFRAAANQVVAPPPPPSTSVTENVTNGTGISGGFKSRFQDSDSEEEGAPFSSSGSKSTQPSSASTVESANSGNGFSLFKHKEEMDGFGKKWGKLSMRSASAGDFGNQQSYSAAKRFGSAAYEGSGYDSKMKSLREEIPREEDLDDVNAKKNAFGKKLKKLFGRYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6N0</accession>
    <name>A0A8J2T6N0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S03-08152g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_08152g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6N0"/>
    <dbReference type="OrthoDB" id="4069585at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.60">
        <property type="entry name" value="Rad61, Wapl domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031550">
        <property type="entry name" value="Rad61_Wapl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038496">
        <property type="entry name" value="Rad61_Wapl_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16997">
        <property type="entry name" value="Wap1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad61 Wapl" evidence="2">
        <location>
            <begin position="211"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="571" mass="64734" checksum="B8B276F7672FEA39" modified="2022-05-25" version="1">MKVYGKRSSSIGIYASHSDRSEVELSDYESEPDVKQGKASKASQEIQDSTVESPMPLGICTDDQLNSLSDAKVNSSASSSVDSSSANLKAFDFLDHTEQLTKRRKTLHQASTDPNDEDTATKLVNDNPFNKTIDDLNKFISSLKNGDESSIQETFKEELEKSLELKSSGDSGKLTYGHSRTILLSKDEEEGLEADEDELKLSKEQKDDSQTYHINELKNMGDNLKYQDDLELLMEGSPTKMSRPHFTSNLLSIALTMNADEDFCCYVNEHQAAEMWRWFFSQRDWNDPVLILMQGWLSTIFHLPADDLPPFFQSFVAASYSNNVLPHSSTVVTKIAQLNYKDFLQRTRNSTSQEYALKLCLQYPGLLSSKELVESIVDNFVKKVKSPKSSFPVIERIVSSALVQGVAWAPTLFNRLVTLLPAYCNDDSLIKCLILLTNERAMPKQNLAFKISMEFVLNHFWPLRLEQTDILILHLGLCLNIIGSSKEMSVDDSQWAQANHTFSKLSHCHEIDCFLPNMFYLNFAYMTVSSNKKLQSRTRTKLAEKLNSFAEEARHYNKSIPDKIKYVSEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6S9</accession>
    <name>A0A8J2T6S9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_04632g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_04632g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Tango6 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6S9"/>
    <dbReference type="OrthoDB" id="39591at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019451">
        <property type="entry name" value="Rtp1_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039600">
        <property type="entry name" value="TANGO6/Rtp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20959">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 6 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20959:SF1">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10363">
        <property type="entry name" value="RTP1_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA polymerase II assembly factor Rtp1 C-terminal" evidence="3">
        <location>
            <begin position="664"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="555"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="561"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF88203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="908" mass="103570" checksum="4FC1F6D7594B7AA4" modified="2022-05-25" version="1">MSVSDILKYPQNVGSTPLDSFFQDLEAKILSKTGSLKADNQRFVSQLLHYLRKLHSLTLDEQAELNAKGSDLLPISLHDMKYVENLIALLIIHGIYVNLPTDFQAPLQFSKDEVPSSYKSDFSTLELVCTTIHSILLQNSDQKDYVRSIILKGPLFMNSYLGLLTLCVEMPSSEHEKMLQELENVQQSYELFEMYTFLVQIRNADAKARLLTQLGTLAVRRNNGVLSLIDFIMGVREDEQVDLEKMNKVAQLLVAKPKSTSSSQYFSSLFPQIYDGLSMLNRPIVVSCLNNVVTTFFLKNQKIVKDFLFQKLYKVIFNEPIRDHCAKELNDTNNVLISLTKNSCNDLLQNLVGGVDKKLFYLNLWTYALFLKRNQKLNPLGQENRPYYEVILTLLKSFVVLLQDNEILTYLSMNLLNYRHEKWEYCIDFETQLAYIALRSEEIELRARKEWKDPLAEANEMFTSIDEAVDLFVQLLRIINKEEMVKDVFLAVLARWVKTDRHNDAALGDIHSSALVLLDLKILEKLHNEFTTDIVKKPTDILQLLIELIDFCSDEERQQDSDDEERSEKDSDDEDGGGENEGDHKIEAQLQSAQPSALDMMIQLLKSVLPTPAAKLESSKISLRIIDAKLLKHGNNSMHKAITDVIESKESAAADNDADDDFLHEAMVNLSDQSAPIEVLGLTQLIQLIQKKSSVVTPTTVTQWHIQFLKSKDPYIYLTAIKGLSILCECDERTFDRLLSMYAGTKKSNNILDDILKIGEVFTNYIQHQNEMFQGPQAHKLIDTCLIKVRRRDEIDDRLRMSAISLLGTGVQTNARGIQDRIGDILDCSFGILQMETKAKGSFVVRRAALHLIHDLLYSAATFPSQYNRAKLVTLLEYTREKDHDMLVRDQSAQLLSILNSGEYSRTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6U5</accession>
    <name>A0A8J2T6U5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA mismatch repair protein PMS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_07954g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6U5"/>
    <dbReference type="OrthoDB" id="10263226at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032389">
        <property type="term" value="C:MutLalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000710">
        <property type="term" value="P:meiotic mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16926">
        <property type="entry name" value="HATPase_MutL-MLH-PMS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03484">
        <property type="entry name" value="MutL_Trans_hPMS_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.100:FF:000001">
        <property type="entry name" value="Mismatch repair endonuclease pms1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000014">
        <property type="entry name" value="Mismatch repair endonuclease pms1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000070">
        <property type="entry name" value="mismatch repair endonuclease PMS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="entry name" value="MutL, C-terminal domain, dimerisation subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.100">
        <property type="entry name" value="MutL, C-terminal domain, regulatory subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="MutL/Mlh/PMS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042121">
        <property type="entry name" value="MutL_C_regsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF52">
        <property type="entry name" value="MISMATCH REPAIR ENDONUCLEASE PMS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08676">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="DNA mismatch repair protein MutL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein S5" evidence="6">
        <location>
            <begin position="215"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="MutL C-terminal dimerisation" evidence="5">
        <location>
            <begin position="717"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="373"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="512"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="383"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="410"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="519"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="535"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00070941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF89591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="914" mass="103893" checksum="B9AB90288349ADE9" modified="2022-05-25" version="1">MSKINAIGSEDVHRITSGQVIIDLTTALKELVDNSIDAGAHHIEIVLKNYGIESIECSDDGEGIGKENYESLALKHHTSKIITFQDVAAISTLGFRGEALASLCAVSNVIVTTTTKPPRADKLEYNHDGTLAMRTTTSRNKGTTVHLSGLFNNLPVRKKEFSKNCKRQFAKCLTTLQGYSIIQEDIKFSVWHITSNGRKNLALSTTRNQNISKRILSIFGSSSMRGLSELELTLDLNPFKGQISKKYEEESDFAAMDYKIHVHGLISRNSFGCGRTAKDRQLIYVNKRPVDYPSIIQCCNEVYKSFNNVQYPAFFLDFELSPMLIDINVTPDKRTVLLHNERYVIDVLRESLMKYYDDQDLLLPKTSFAQPALFNPGQKRDLEEDEEEQDDCEEVNNRGNPKKRSSRAVNETRCREKGDLETTEHPRRISIEKEPMSMTEGAPEKKLFTSSSSQSSEEENEVVEPSKPCAELSSVDTSISTTNLLNKFANLNPESESPIANEDKHLKTRATLNENLRGSPSITSKSTQDSSSTSRNLQQFINPLQNEPKSDKPENETQGNQSDSVVIEIDDTKVEHRATLMPNDQLVFLSDQQQNTRRGNFEKVHSDDEDAEFASMEPPEINVRVPLPSLNTDRFAHHTYRSLIEHGCSNEQRTESIMVTLRIGNLNFQKNNSIRQKMDREQHQISFRRNENLDNVQEGENYLTLTVKKADFKNMQIVGQFNLGFIICTRRTGENYDLFIVDQHASDEKYNFENLQKNMAFKSQRLLAPLVVDLSAIDELAVIDNLSVFEKNGFKLSIEEDEFKDTKIHLLSLPVSKSTLFDVNDFYELVHLVKENQGINRDSVKCSKIRSMLAMRACRSSIMIGKPLTKKTMSKVVRHLSALDKPWNCPHGRPTMRHLMELKDWSSFHDDYDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6W0</accession>
    <name>A0A8J2T6W0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ZYBA0S03-12860g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_12860g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T6W0"/>
    <dbReference type="OrthoDB" id="2018619at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11482">
        <property type="entry name" value="SLC-NCS1sbd_NRT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF15">
        <property type="entry name" value="NICOTINAMIDE RIBOSIDE TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="360"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="425"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="470"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="511"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF89255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="617" mass="69934" checksum="37234E5990C4CA22" modified="2022-05-25" version="1">MDSDKQIEVLRSVVSKNDVEILGDEEKLQKSVESNTWWQKFLKKLEVPHDANDPISVLRNPDLRPIQDKERTWGFWSFFAYWGLPNFAVATYSTGSALLALNLNIQQSIGALVIANVIIALFTILNSNPGIKYHIGYTLDQRMVFGIYGSFVGIIFRVGLAIVMYGYLAWLGGLCFNMVFSSFSKNYLNMKNTFPESVPMTRRDCIGFLCFQVVQMPFAFVKPRRVNVPSIVSCFMTLFAIIGMMAYLISKNGGPGPLFHEKVTLSASKRSWMWLYAITIWYSGVSSGVTNQSDYSRFASKGKPCYYGLFLGTVLPGTFVSLAGMLCASACKQLYGVAYWTPDEIVEQWLNDDYSAKGRAAAFFVGISFTASQLFLNLTQNGYSCGMDLAGILPKYINVTRGTLFVQLLSWVVQPWTFFNTSSGFLNAMSSFGIFTTPISVMSIIEYYLVRKSKLTQLDFFTLSKEGAYWYHYGINWKAMLCLIAGVVLGIPGLVYEVHEEIVPNTGMLNFYYGYMFFIPLVTGGMYYGLNLIFPFRHQKLYQEDPIDYFNCYSEKELEKMGMLPFSGDQENLYNILSETHDHVEEYDADNISDHDLDNKQNMHIVTDSKSKCSDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T719</accession>
    <name>A0A8J2T719_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alpha-mannosidase</fullName>
            <ecNumber evidence="3">3.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_05798g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Degrades free oligosaccharides in the vacuole.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.24" evidence="3"/>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T719"/>
    <dbReference type="OrthoDB" id="10261055at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009313">
        <property type="term" value="P:oligosaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000004">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000002">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000001">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054723">
        <property type="entry name" value="Ams1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041147">
        <property type="entry name" value="GH38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017:SF1">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22907">
        <property type="entry name" value="Ams1-like_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17677">
        <property type="entry name" value="Glyco_hydro38C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="9">
        <location>
            <begin position="564"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF89502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1079" mass="123787" checksum="463F841A7AF49BC6" modified="2022-05-25" version="1">MPLQEICYDPKFKPVNSIYENRLRQFIDNGQYKRLNLPKFYDRQRIHQDGHHVKIWYHQIPFERGSDPTSPDKRPSWRSVIDADKAGKIEWKNTYGNQPFGPSWSTTWFKVKLTIPKEWLESGDQLTFEFDCGNEGIVIDPETLVPATAFSGGERKEYQLPKSEDGKYSFYIECGNNGMFGVGAGGSNNPPDPNRYFHLVTADIVWPDWDARALFFDFWELGDAARELPGDSWQKNRARQVANLVMDMFDPEDRGSVVKCREFLKKEYYDKYNDSDKVYSKGDVHTLASVYAMGNCHIDTAWLWPWAETQRKVVRSWCSQATLMDKYPEYQFVASQAQQFKWLKDQHPEFFKERLIPKVQQGQFFTIGGSWVENDTNVPQGESLCRQFLLGQRFFLKHFGVKSVIFWLPDTFGYSSQVPQICRISGIDRFLTQKLSWNNIDSFPHSTFNWVGIDGSQVLTHMPPGNTYTSDANFGDVLRTSRQNKSQEFFEPGLLLYGKGDGGGGPTREQLEKLRRIRSMYNRNGNVIPKLQMGTTIDEFYDKIMERTNQGNDLPSWIGELYLEFHRGTYTTQARMKKLMRTSEIKLHDVEWVATKTSLLHPDQYKYPARKINDLWENVLLCQFHDVLPGSCIEIVYKYESIPMLENVLKECDKLLQGAQKVLSNCKPSSKQQVQVGTLSWTKNEKLAVDSSGFIPATLKEDGDAFILNNGELTIKVNKESGVITSIVDDKTKTEYIDNKRGRNKLGANQFVLFEDEPTSFPAWDTELYSVNKYKYLTKTERVDVVCTSDEKVTVEVVVPVEEGCKLKTQITLKAKEAGVSDESKVDIHTEVENWNAFYQFLKVEFPVNILNDFASYETQFGITKRPTHYNTTWDTAKFEVCHHKFADYSEYTKGVSILNNCKYGFATHGNLMRLSLLRSSKAPDGNADMGNHEFDYAVYPHSGALSHKTVKLGLEFNYRYKFALASEVAKTFEELITISGDENVVLSNIKRGEDDKNIESQYALDSKDEKSVVVRVYESLGGESTATLKTSLPIKEVHVIDSLETKSLGTIDFNKASESHDIPIRLRPFEIGTYKLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7B7</accession>
    <name>A0A8J2T7B7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_12200g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Required for vacuolar assembly and vacuolar traffic.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the VPS41 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7B7"/>
    <dbReference type="OrthoDB" id="244107at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057780">
        <property type="entry name" value="Beta-prop_Vps41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016902">
        <property type="entry name" value="Vps41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 41 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23411">
        <property type="entry name" value="Beta-prop_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23556">
        <property type="entry name" value="TPR_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028921">
        <property type="entry name" value="VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00299">
        <property type="entry name" value="CLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Vps41 beta-propeller" evidence="7">
        <location>
            <begin position="81"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="5">
        <location>
            <begin position="666"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="24"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR028921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF88521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="891" mass="101918" checksum="F5741401F8438D27" modified="2022-05-25" version="1">MAKKTPLEAVYDGSGQVHPAKLLENGRIDGKEKSPQDQHHDSVRGKETSITTEKDSFQISGSDDHVEESESEDEDNEPPLLKFTRITKLPKTFRRDAISTCLFHDKIFAFGTHAGVLHLTTTDFTPIQTLKCHRSSILCINTNGTFFATASIDGTVAIGQIEDAKNITLFDFKRPVQAVVLDADYKNSKTFVSGGMAGEVILSQRNWLGSRIDITLAKGNGPILGIHTIDDIIFWMNGAGITFFSIHSRTQLLNVPFPIESKDGLRPDLYRPQVHCPETDRIIVGWANHVWMFKVSLRQTTDSSNNLGAILSTAASSLRAVPDKKVELEHYFVVKMLIAGISSFKDDQLMCLGFEDSTGEGSSLSKIPEITIIDIFTGEETHCDEVVLNNYEQLSLNEYHLGKYIGDSSPEYFLVTPNDAIRIQELSFKDHYDWFLEKGNFMRAWEIGRYVVNAFERLETGLNYINQLLEAKQWSEGATNLSIIISGTSIAEDDAFKDCAISKWTDMFMRIVKSDHVDVAAKNIPIAPQLGTCLYDAILEHFLKNSRLENFLHYIQLWPLQLFSIHKFENELEELIEKDNDLVHYYRDALIHLYLEENRFSKAMPHMLRRKDMRALTVLLSHNFLSQYKDNLLDIILLPYDGKLEELSRMPIVEIEHKFDKSLELLVRNRQSLHVQQVMSLLSDPKELRILLFLYLKRVSSIDPLSTAPFETDMIELYSEFERPALLHFLKTKTNYDVEKAIEICSRNKDSYNELIYLWSKVGETKRALSLIIDELNDPKLAIEFVKNLGDPELQEFMVRYSMDKPKFVKALLSSPDEFGRTYLEVIKGMPETMEIDDLHKILMRITKENSLSLIVGTSIFKIIDDETIEYATELLRIRCLGKVFRIEEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7H3</accession>
    <name>A0A8J2T7H3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ZYBA0S06-00980g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_00980g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5330228at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18070">
        <property type="entry name" value="DEXQc_SHPRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23135">
        <property type="entry name" value="RING-HC_IRC20-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052583">
        <property type="entry name" value="ATP-helicase/E3_Ub-Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059033">
        <property type="entry name" value="C144_05_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45865:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SHPRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45865">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SHPRH FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26021">
        <property type="entry name" value="Ferritin_C144_05"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="360"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="1193"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="1315"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="151"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF90119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1511" mass="172004" checksum="FEE29233784927ED" modified="2022-05-25" version="1">MSRCRPLDVPVNCGRDLSCEKRDLGVQALCLVEEGLFPFLSRCEETRKKRDADVKPKRSKNVQKRCVEIACLRVHSDGDSVPDIPTGSMRGDILAAEVPASMQFLEVEDSDIVMVSAYGEKVLKVQWKSCDDRVRQVLKVGHNKCLEQEVSNAAAHKSERTSPMARPRSRRKKSPAQDAVVAAKRLVSRTFKDLNCEICLEAQSSGGWVLRIAVSLMYLPQTMNKFSHETKFLLDTLFARDGGTAEPLPPSPFIQSKFVAQTVAYTRGRLTAGAHRGISGLQLHLLPFQSETVEWMLSKETHGTLPHVLMGEDDILRFLNQRVSYGYEAVSGNTFWNKFTNFVLTRKEVDDILADMLERHGDSDARARGLLCEEMGLGKTIEVLSLILLNRRRAVLPRTFVAEDGKTILHTKTTLIICPNAILQQWVNEVEAHTSGLSIFHYKGFLSVKKFANTDNISEIVEQLSQYDIIITSYNVVSMEVHYAEYNASSRPRRTISPIKYDYSSPLSLMQFFRIILDEVQMLHSDSTKAAKCTSLLHRVHTWGVSGTPIQLVKDFQTVLSYLQISPFHELPEIVAGVNNNVVHHQEQQLVQGVRFTLSELLNLFIKQDLSVRHSKEEVMKQIHIPKQHNYIMPLEFAPVEWDNYLDLWNTFLTVSGYGASGTGNPRLTNAQLNQWLARLRYLCCHAVIPDSHLSNTRNQGNSSSATLHNIDDVLRLMTADAVEKLDSLYRDNYLLQIKSAQAKTELQNNPEGAIKLLQSVKKNLYSDLREKCLITDPLDTTKASLEERDGSPLARDLSEGASLMRRRAYLDLLHQCFFFIATAYYNKGSSKLEEVDQENEQLALKNSNEKPKTYSEVYSMPEMEEIEQNQLLEQQYYSYAERLRKRILIGRVQKVHFSVKEIQKYFQENPAFKPQSLQMIEFDDGGNYSGTMLVSRCFTLLASIVTSLNEQAMQFNGLFAELKQLVYAPILKNYDENNEDDKAQEYSSSIDDQDKMFALFACMEQLLANRDAISTSDETIKISKNFLKPQAGQKFSEYHSKLLSGLNMISGSPLKPIFDELKNAKVVRNVSCSTRSGNNTKEDFEDYLLRYESQLFRMTKENKGMRESLKKLNSVYNAKLEYYSQLQKISDSLIPLLQLENAARSTIVKSIKDDTQYRRNLEKISSAESRVKYLTGLTKLKESIEKNQKFSCAICLGTIHTGSIIKCGHFFCRKCIHSWLKSNHSCPLCKTKATLLEVYNFKFQDEPDRGVSPSNDVYMDEGKSCSTNSEEIGSSSFSRENLDPVLREKYSLFPQFDEVHKMVIKESFGAKIDFVIKLILFLKLKSETEENRYPPQILIYSQSFEFLKVITAVLNIHEIKHLSCLTNVTNVGEAISRFKNNSSITCLLLNVKSLGAGLNLLNARHVFLLDPIINRGDELQAMSRNNRIGQTEETYVWNFMIKNSVEENILKYKCLLEGRRKLLGQKSRVKQEELDATAVDEEREELEMNESTGEQVSGRHLKSCFFSCNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7M5</accession>
    <name>A0A8J2T7M5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ZYBA0S06-07338g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_07338g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7M5"/>
    <dbReference type="OrthoDB" id="10006270at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF9">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="290"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="464"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="566"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="607"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="641"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF90385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="752" mass="84366" checksum="F748A3F154CB8CC9" modified="2022-05-25" version="1">MSTHGTPTQHNSTLVISPFITGTNGAKNNMSTERTTAQGDGHRPTGEQQSGGAPQNWGDAPTNSPYQSIANSPLMQKTNPSAAAASLATPHHNAMDTGSGTGILTSISKNSLFGSTLPSTLRKVSLQREYKDPPTIDKDVDPSAEPDMGTTTLTTTATTTATTVAEFDISELSSIEKLRLWRHDALMQHLYKTAEFVGNKIYTMTGDPNDAFWLAQVFYSSGACIRAVELLSKDMLDSVGIMCRYLLALCLVDLQRYEEALDVVGETNPFFEAADQEAKKQNDGGIKLESSLCYLRGKIYSAQNNFAKAKESFKEAVLVDVKNYEAFEELIAKNLLTPREEWDLLESLEPEFAVLDDNQDMIKCLYAIRLSKYLNEDKTLEARKRLINEHSLGGNIDILRSQAEIFYTKCKFSQCLEVCETILKRDEFNSEVLPLYISCLYEVGAKNKLFLLSHKLAENFAKNSITWFSVATYYMVVDKIEEARKYFSKSSILDPNFAPAWLGFAHTYAIEGEQDQAISAYSTAARFFPGIHLPNLFLGMQYMALNTFSLAEEYFTLAYDTCPQDPLLLNEMGVMYFKRNDLHKAKRYLKRALEAVKDLDPTSRTSVSIQMNLAHAYRRLGENERAIKCFKTVLEVSSKDSDVYCSLGFLYLKTRELQRAIDYLHTALSLKSSNLAAQELLLHALELNVSMNLDSDHPLMVNAQLYESNFDQFISSRKRAPMTLDSVGYAKRMRKTRGSLQIPNEDDAMDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7Q5</accession>
    <name>A0A8J2T7Q5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S05-03290g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_03290g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF89853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7Q5"/>
    <dbReference type="OrthoDB" id="48651at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="472"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF89853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="483" mass="53235" checksum="FE798BE1F49BC49E" modified="2022-05-25" version="1">MSLQEFLGDDTLGDSVWNEDDINLDAISNTTSIDVLKNKTQKQQQQQEGRSDAHHPFGYRSPPSMDGHPSSFAPAGPPYIVKFSQLPPRFADGDIHDLFHEKFTKFVKFKLFWELNANPSIAVLNSGSVFDQNFKRENKVAFVELYSGRDMDKILKYWTTPMLKLYNIKVEPAEFSDFNEYMAKVKLLTDPKDDAAKPYVPLQASKANAKPAKPKPNPFGSAKPVDTQSKILDIEEKVGKLHVEDTSTLRRISASEPSSQHPGKVTLLKKTHEENNVSPPAAASSALVDDPPAKPLSYLQVIKKTAEESKKSPSTSSATTRISSPATQSASLEDGAQHSALEDGESFEDTDYREGRPFHKRNSFSDNGGSMRAGRGGSRGGNSFRRGGARGGGRGGTGSRYQNDNRRGGAQGHNSHNQPFHQQDGSKHIPEEKRYSMFKPASGFLWENGNDKTANGQNQRGGHYGRGSYRGRGNRRGRGFSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T862</accession>
    <name>A0A8J2T862_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S05-05556g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_05556g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF89954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T862"/>
    <dbReference type="OrthoDB" id="6781668at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052587">
        <property type="entry name" value="TELO2-interacting_protein_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059075">
        <property type="entry name" value="TPR_TTI1_2nd_yeast"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057567">
        <property type="entry name" value="TPR_TTI1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057566">
        <property type="entry name" value="TPR_TTI1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016441">
        <property type="entry name" value="Tti1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049362">
        <property type="entry name" value="TTI1_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460">
        <property type="entry name" value="TEL2 INTERACTING PROTEIN 1 TTI1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460:SF3">
        <property type="entry name" value="TELO2-INTERACTING PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26245">
        <property type="entry name" value="TPR_TTI1_2nd_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24181">
        <property type="entry name" value="TPR_TTI1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24173">
        <property type="entry name" value="TPR_TTI1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21547">
        <property type="entry name" value="TTI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005250">
        <property type="entry name" value="UCP005250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTI1 N-terminal TPR" evidence="2">
        <location>
            <begin position="8"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="TEL2-interacting protein 1 second TPR" evidence="4">
        <location>
            <begin position="371"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="TTI1 C-terminal TPR" evidence="3">
        <location>
            <begin position="786"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="777"/>
            <end position="797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF26245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF89954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1034" mass="118009" checksum="DA8A227DAA1EF2DC" modified="2022-05-25" version="1">MTSQSVAFQELRPLCVELSRLAFLPPQSFDPQSFILLSSLKRLDSKLSTFPDESFSQNLAEYVFVPITGLLKHSRLGESQTEYVLRLISQLLRLSWTQRGTFAKELAEQLLPLLTYLISQEQENSTLNENSTEFRAAGCQALFRFFISIREQCYKSEFFSSSNTKSLSSLGHCITILLDILEGIPENPKIQLRCLDTLRILYQDIVGDGEILSFVLPGTISTFVKVLVAPGLTVNYKVVIRTLKCMEIMLQIIYNDISLGTRRDPFTDLDEMLTYQQKNPKWHMNERIEIDMENALQHRNLSWLLGTSSQIKRALENFIPKLLKRQHPEINGALVSLVSVLFDNCNNSLGNCEAVFVSTLVRIRQDPKSHLGDHIRTLKDLVKQCLFKLSDIISFQNKQELLSFSYAIDVLNQYDVDKDFSFIRDIATKIIDTLESDLLQTYSKSQDKKILEQNSSVILANNFEDEIAASGNEVAILPRISKELLKTLGQLIMSTGMFMADESRLELFIESLLSEQVCATPVRKSLSLWFSTWAMRGLYGEHRAGHLTDFLNLDQDNEHSFGPCYMILEYCNSFAQEITSAYEGRSISPQEETAMSVIFFSIETVCSIMHTEFAPELIDCVYLVVENLASPSATARHFAQTCTLTISVELYHGSVREAILSNIDYLVDAISTRLNLGMTERVSTVFTVICRIAGYETIETFQDVIETIFKMIDYYHGYSELCLQFFQLFEVIVLEMKKKFMDTDRPTIALQDDHSVRSSYAPWGMMNLQQLFSVLDKPSSGEDQTEEEEPSSFQEYFDSKIQEVDSDDEENHGDEEMELQAADREEKWISPIPVSSYRILLQIFNYGDRLLTHPSKHLRVQILHVMELIIPLLSTQYDSLLPQVANVWDTVVSCTLDQDYTIVRPACRCLQMMVHHSGDFVTKRFFELWDCWCDRSLLLREVRSTGKMEDVSFQVCALRKFPPITQGALLALAELLLEGVAVTELLLADSALKDILYCCIQILPAEMVAQRSILLADVVWVLKNLELNSKVKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T887</accession>
    <name>A0A8J2T887_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ZYBA0S03-08350g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_08350g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. CAR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T887"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015203">
        <property type="term" value="F:polyamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010509">
        <property type="term" value="P:intracellular polyamine homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000009">
        <property type="entry name" value="MFS multidrug transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF5">
        <property type="entry name" value="QUINIDINE RESISTANCE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="458"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="498"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="540"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="572"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="634"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="111"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="308"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF89066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="674" mass="75475" checksum="631EC73B88E22E67" modified="2022-05-25" version="1">MLPEDADTPAANTADANEHGGVTLNTLISYSELESSQYHPQQYTNKNEIQEGEAHSTNPPAGSQNRSVWNRLKDRTFWAARKDRRGLLPHLAVIPEFKDARDYPPAIKKFIVFVIAFSSMMGPMATSIIFPAINPITDDYKCSSIMVNISVGVYQISLGVFPLWWSSISEMNGRRSVYIISFILLVGFNVGAAVAPSIQDFIILRVCSGGAAASVQSVGAGTVSDLYVPEERGANLGIYYMGPLMAPLVSPIIGAALVSHWSWRSTQWFMVILSAVNVMVLTLFLPETLRKQDNDAAIAAILKERRKGSSSADEKEDMEAEDESRKETPDCGTRSLSDAGRSIASHKETDEEIYELQRALSQASNNVNRFTLNNDLDMNAPQMSRIQSYDPKQEQMLRNFEIQRLQSNMKEELSKIETSKSKNTGHIIVDQRSRWEKFRHMCYLYLFRPLKSIYFLQYPPVAMAITFSAISFAILYFVNMTLEYGYSHPPYNFKPMLVGLMYIPNSVTYMLASIFGGKWTDRLLKNYKAKHGILAPEARISWNLVTAVITFPISLVIFGWCLDKGEHWETPLVGTALFGWANMMTIGATVAYLVDSLPGKGATGVALNNLVRQLLAGAAVFVNDPMIINMGIGWAFTMLAFIIIGASSVLLILKKYGDYWRENYDLERLYKKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8D4</accession>
    <name>A0A8J2T8D4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="9">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_05996g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RSP5/NEDD4 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="9"/>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T8D4"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000011">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000017">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000053">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000156">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024928">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF440">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEDD-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001569">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="C2"/>
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    </dbReference>
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        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 9 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="231"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="333"/>
            <end position="366"/>
        </location>
    </feature>
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        <location>
            <begin position="389"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="15">
        <location>
            <begin position="478"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="135"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="259"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="106"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="137"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="199"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10 11">
        <location>
            <position position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001569"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001569-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CDF90328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="811" mass="91931" checksum="07B858A88B895E24" modified="2022-05-25" version="1">MPSISVKLVAAESLYKRDVFRSPDPFSVLTIDGYQTKSTSAAKKTLNPYWNETFKFDDVNENSILTVQVFDQKKFKKKDQGFLGVVNVRIGDVLGHLDEDTTGGARPREETITRDLKRSNDGMAVSGRLIVVLSKQRSTASSSTARTSSRSSADTSGAAATSTTAAASSGTSGSNSGSQRAGTSSTRNHTRSHSGARTPRSTAASVEQTLQNGAANAMSTRQYSSFEDQYGRLPPGWERRTDNFGRTYYVDHNTRTTTWKRPTLDQTEAERSGQLTANTEMQRRQHRGRTLPSGSNDSSSVTVQTGPESQTPAVNGSAAAAFAATGGTTSGFGELPSGWEQRFTPEGRAYFVDHNTRTTTWVDPRRQQYIRTYGPTNTTIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQNVPQYKRDFRRKVIYFRSQPALRILPGQCHIKVRRKNIFEDAYQEIMRQTPEDLKKRLMIKFDGEEGLDYGGVSREFFFLLSHEMFNPFYCLFEYSAHDNYTIQINPNSGVNPEHLNYFKFIGRVVGLGVFHRRFLDAFFVGALYKMMLRKKVVLQDMEGVDAEVHNSLKWILENSIDGILDLTFSADDESFGEIQTVDLKPNGRNIEVNDENKKEYVELFSQWKIVDRVKEQFRAFMDGFNELIPEDLVTVFDERELELLIGGIAEIDVEDWKKHTDYRGYQESDEVIKWFWTCVSEWDNEQRARLLQFTTGTSRIPVNGFKDLQGSDGPRRFTIEKAGEIRHLPKSHTCFNRVDLPPYEDYDNLKQKLTIAVEETVGFGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8J2</accession>
    <name>A0A8J2T8J2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ZYBA0S06-06018g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_06018g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T8J2"/>
    <dbReference type="OrthoDB" id="128867at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF90329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="484" mass="55245" checksum="EC2FBAD5899A60AE" modified="2022-05-25" version="1">MPPELPGFYYDEERGRYFRVSSTRQRPSDPFSSYDIEEVKKRKVNQEQELELHELSESRSKIYQDYLLQLSDPFQRAFGSDIPSHFIEGLKIQDSDFDDYSANVNSSFGSNVLRHEICSISLLCDLNSDEESLLIFATRKGLVEECVRENLLEKNLCDLSSFQNFGGDCNFHRLTITDYDPRVLYFHASEEGSNTHFFGLIGNDKNTVKRQKFKQHENVCDSVNLGEFYVVAVGRRLRLYSWKKRSVLIDNPLDNKVNSDILTLAIGKSNGLAKELYVGCRDGSIYIIPIGPDCSLEFTARRRLSFANALSIISIKTTETLGIIFVSAVGGNKQVLFMLDTLMDNCKPLVKFQTTFLNLTKTSEIFDVSTEGHYICYGSLTARNGKGDVEIFSSYLQDNLKYERLETMVFYPLRSLSREILQPLGLRDTKLCAAGLGRQPRYHHNTLPVHGCRIVSCRLYLVLEETSSRLPETLPRLFLVSTDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8W5</accession>
    <name>A0A8J2T8W5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ZYBA0S06-03664g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_03664g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="5290825at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015798">
        <property type="term" value="P:myo-inositol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015791">
        <property type="term" value="P:polyol transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF25">
        <property type="entry name" value="SUGAR TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G05830)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="448"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="484"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="513"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="545"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="577"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="154"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="652"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="656"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="674"/>
            <end position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF90227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="694" mass="78780" checksum="C3688081CBAE9827" modified="2022-05-25" version="1">MTRDLHSYRNLHQPAECEMDDKDEQFVEKSEPLFQQVTHDELATTHTPSPSKGDAEEDGDYSKLEKEANKEKNVSARGNKLAALPAERVAQMGREFAEQYELPNDDDIFGRAAMLAREPENYSQMDFLSEEEKAACHAEVTRPWTSMTRQLIGIICAAAMSAAVQGMDETVINGAMLFYPKVLGLGSGSKRDEWLEGLINGAPYLCCAGFSCWTTDWLNNRLGRKNVIFVTCAVSAITCFLQGFGPTGTRGWHYLFAFRFLLGLGIGPKSATTNVYLAECATKQIRGCTCIFWQFFTAFGIMWGYVFTLIFYRVKPNGIHGGLNWRLTLGSAMIPALLVMVQIPFCPESPRWLMGKGRYKEALENTIKIRPHRLLACRDLFYQHVLLMEEESLQLPFWIRLKEMFTIRRNRNGLLASCICAFMQQFCGINVIAYYSSSIFLDSGFTEISSLCASLGFGLINFFFAIPTFFMIDKHGRRYLLLRTFPWLALFLLIAGFAFWIPGEQARTGVVSMGIYVFSAIYSFSCGPVPFLIAAESANLASRSLNSSIFTVVLWGFNFILSVTWPSMRRAMTAQGAFGFYAFWNAMGFFMVYFCIPETKQYTLEELDEVFDVPLRTRIKYNWLEMWPNFQYHVLRRKNVMRRAPLGPQLPSLSDWEEKAEQEHVERAASLSSDRSDSDTSEAEHEVSGSGRDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T976</accession>
    <name>A0A8J2T976_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_04104g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T976"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045719">
        <property type="term" value="P:negative regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060917">
        <property type="term" value="P:regulation of (1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14004">
        <property type="entry name" value="STKc_PASK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000320">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000314">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF51">
        <property type="entry name" value="PAS DOMAIN-CONTAINING SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="962"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="82"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="797"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="912"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="110"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="797"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="832"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="845"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="912"/>
            <end position="938"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF90569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1222" mass="136452" checksum="F2B433ED6B7AD8A8" modified="2022-05-25" version="1">MPYVEASSDSGTSYSTLRERHALGRPSGFSGSGSPSISPKTSRDSLDTYNNTIPSIADTTSDNCFPKYMPYKAGAINDSNLSLVNDDEDEQRSLSRELKLQGPPRHPLGSPSKSQSVNSLVSSTTDTEMDELYSLPNESTHTYSYNPLSPNSLAVRLSILKRSLEILIKNPNMLEEPVEQTRPRYGRNYSDAGLQHVKQCDPGKTVKQKGTASAAALKAFVSNTSGSGSIPVTPASSSTDTNLLPPSTICRNLQRASSVVFPLDYCKNPETANSERSDLEDLLVLLNRAIENNDSEGVTDLHMMSLLNINKLILGKDGQEEDLRTLHLKRALLDSLAEPFFEHYNTTADGEVCSKDEDTESDSGNEDEDFLEFNASAGVGRHCGRVLHTFTSGKNIAPQAIFTCSQNHPWAFRAANDLACLIFGVSNQALKALTLLDLIHTDSRNFVLNKMMSTEDQELVFSGEIVAIAQPGQGKNSSNLIWASFWAKRKNGLLVCVFQKVPCDYVDVLLDVNDYSIKSISGGNELFWGESGQNKVTNENCGGDKHDKNTEQGTQIKKKTVKFANDRREIKDISVSLARLIEDVLESKVMAPDDDLLPMPIRVANHVNDTRYFTLNNSSSNIPGAVALSILEDNLKLKIYSLPYEAGLFVVDSHTLNFISCNKSILKNMFGRHFADMLGKPMTDIIPSFVRMMKFLRENYSSLSITSRKNKGLVLTEHFFRKIQAEMMGNPESFYSSVGIDAVHRDGFKIKIDLQLRVMTSRVVLLWITHSRDVVIRNYTSTPSQLRMLKENELSYVSSTPSSGSSSKKSSSKISLSNLKEMASRSAKGADISNSAETRSANNETEVKAQTDESSELKISPHVEDIDEYTTHVDVGDPELQRKLELTKIYSKDKFRFVNDGNFKLDQDLIKSITSPPRNTGLDSEPDTPTSMTRSNSLDPIFLRTPNPNIGAQKHTKKYSDFIVLQKMGEGAYGKVNLCLHKKQRYIVVIKLIFKERILVDTWVRDRKLGTIPSEIQIMAALNKHPDENILMLIDFFEDDECYYIETPIHGETGCIDLFDLIELKTNMTEYEAKLIFRQVVSVVRHLHCQGIVHRDIKDENIIVDSKGFIKLIDFGSAAYVKNGPFDVFVGTIDYAAPEVLSGEPYDGKPQDIWAIGVLLYTIVFKENPFYNIDEILEGNLKFQSTEGVSEDCINLIKKLLNRNPSERPTIEEVYNNDWSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T979</accession>
    <name>A0A8J2T979_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 5B</fullName>
            <ecNumber evidence="3">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Translation initiation factor IF-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_04962g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="3"/>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T979"/>
    <dbReference type="OrthoDB" id="4928at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03703">
        <property type="entry name" value="aeIF5B_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000013">
        <property type="entry name" value="eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000112">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003078">
        <property type="entry name" value="PRK04004.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="14">
        <location>
            <begin position="403"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="176"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="267"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="278"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="315"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF90607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1002" mass="112490" checksum="234B69CE45F6EA8B" modified="2022-05-25" version="1">MGKNNKKNQQSYWDEDFSEDMPASEEFGTGEASTEATPGFDVTPSAEEAGAEAIEADFMSTLKKSKKKQDKKATEEKAENSNAKPVLKSKKEKEKEKKEREKQRKKEENNKKKAQQQAQKEKNKELNKQHAEKVAAEKEAKEKHKSASPSPVPSTAKQAPKKGGKKAPAGLAALKRQLELKKQMEEEEQRLREEEERLEREEEERAAAVEREREAAKEAKREKEKAKREKLKAEGKLLSRKQKEEKRLQEKRREALLSAGNITVAALEKKETPTERTKPKKVVYGNKKKKKTDAHEDEASKPAENTKAEKKEDEKDVEEEVLIDDWENLALDDDEENETVAETEPVEEESTTEIPEVSETSREVSREQSVPAAVFKQPANSEVEHIKPARSSTSPAPDNQKDLRSPICCILGHVDTGKTKLLDKIRQSNVQGGEAGGITQQIGATYFPISAIREKTKTMAKYEKQTFDVPGLLMIDTPGHESFTNLRSRGSSLCNIAILVIDIMHGLEQQTLESIRLLRDRKAPFIVALNKIDRLYDWKSIPNNSFRDSFEKQSRAVKDEFQTRLSNIKLALAEQGLNSELYFENKNMSKYVSIVPTSAVTGEGVPDLLWLLLDLTQKRMSKQLMYLSHVKATILEVKVVEGFGTTIDVILSNGILREGDRIVLCGMNGPIVTTIRALLTPQPLRELRLKSEYVHHKEVKAALGVKIAANDLEKAVSGSRLLVVGPDDDEEEMMDDVMDDLTGLLDSVDTSGRGVTVQASTLGSLEALLDFLKDMKIPVMSIGLGPVYKRDVMKAGAMLEKAPEYAVMLCFDVKIDKEAELYAEQEGIRIFNADVIYHLFDAFTLYQEQLLEQRRKDFLDFAIFPCVLQTLQIINKRAPMIIGVDVMEGAVRVGTPICTVKTNPTTKEKQVLLLGKVVSLEINHQPVPEVKKGQTSAGVAMRLEDPSGQQPIWGRHVDDKDPLYAAISRKSIDTLKDKAFREQVSKGDWLLIKKLKPIFGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T984</accession>
    <name>A0A8J2T984_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S04-10946g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_10946g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T984"/>
    <dbReference type="OrthoDB" id="3163292at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="1">
        <location>
            <begin position="24"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF89716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="704" mass="82410" checksum="85C7F293D95DFE97" modified="2022-05-25" version="1">MVATKYNYKSSAGHEENEKRALRSCVRCRKNKTKCDSMERRPNPCTSCMRKGVECHLDYVTPPQRSKEMKSLYESIRFASDKISTLCMVYDDFMKKFHLNHMRGTRRGIDCNKFPTRILKVKEQFFSFNLNPVDGSLYLNNFRIRSDFLKQSFANFRQIVNSLIKIYFKWERIEGSSMDKVEIFVSQLNPNYLLQTNQLLLLLCILNFYYDIPGLKYLNIFEYIIESYYVGASENSDVIENSKFFTRSNLAKLVAGNLPNGQFHGEVFIKHFTIFLFLNIILYGPEYFMGCFMDKYIRTLEFLRKKINFDKNWEVKWINFYIRLLNLVEAVCPFVGGSKYAVEEEEIDFLFSLVNKEREVDSESNITLDCFICLVKFDQYLIERRRWPRNEKVCKSFAYICRRLTDDLYEILRIRSDESEQGERLSFVQLFFTQLLTLNNLLCANHCGGSVVPFEQENLFNGFNFEIYEVLPEELCGNVEGGEIQGCYYWCQSKYEFLSELLMRNEDMDVSELIFLFVEVSVGKHCLKVDESVSVLRMLSKEFEFFCNRNDMSLRILKSSCRLVWLLYEFVVFWDMLDHIVVYEPFLWNAQLLLENNGLQSQFGDHRSQHYSEVPMESSGGMDMVDEDCGGAKIWNDTVVEDESVGSEEDEDVIGEGCVDSGVGDGNNEEQLSLNGGINHILQNVDWVKESADEVFEKIHKVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9A3</accession>
    <name>A0A8J2T9A3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Elongator complex protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_01420g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). The elongator complex catalyzes formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ELP1/IKA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316464">
        <property type="protein sequence ID" value="CDF91374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="40048at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056167">
        <property type="entry name" value="A-sol_ELP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056164">
        <property type="entry name" value="Beta-prop_ELP1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056165">
        <property type="entry name" value="Beta-prop_ELP1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006849">
        <property type="entry name" value="Elp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056169">
        <property type="entry name" value="HB_ELP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056166">
        <property type="entry name" value="TPR_ELP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12747">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12747:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23925">
        <property type="entry name" value="A-sol_ELP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04762">
        <property type="entry name" value="Beta-prop_ELP1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23797">
        <property type="entry name" value="Beta-prop_ELP1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23936">
        <property type="entry name" value="HB_ELP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23878">
        <property type="entry name" value="TPR_ELP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017233">
        <property type="entry name" value="IKAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="ELP1 first N-terminal beta-propeller" evidence="8">
        <location>
            <begin position="1"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 N-terminal second beta-propeller" evidence="9">
        <location>
            <begin position="427"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 alpha-solenoid" evidence="11">
        <location>
            <begin position="725"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 TPR" evidence="10">
        <location>
            <begin position="950"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 three-helical bundle" evidence="12">
        <location>
            <begin position="1126"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1202"/>
            <end position="1233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR017233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF91374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1337" mass="152047" checksum="20D7FF83D945661F" modified="2022-05-25" version="1">MRNLVTLNKGSLKPLASLSQDATFDVPFELLDSVFDTLSDSITCVLGNLDMGQVEVQQYTKQGAKNILASFTVDSFGDRLLSFTHFADISQLVFVFEQGDIITVTYDPISYNPQEALVEIAGTIDSGISAAQWSYDEETLAIVTRDGNVVLLSKLYEPIAEHHLELEDLKKSKHVTVGWGKKETQFRGRGARAHEREALASLKASGLKGNELRDPTMPYMVDSGMITLMDNRQVSISWRGDCEYFAVSTIEVVADPDDVAQTIERRAIRIFSRDGCLDGASEPVTGMEHNLSWKPQGSLIASVRRSTHLDDEANLEVIFFERNGLRHGEFETRLDLDETIQDLCWNSNSEVLAVVLQNKIQLWTTKNYHWFLKQEIYDDDIKFVKWHAEKDLTMIYGNSQYINVVDFSYKMTQGPTLEPCDNGTSVVIDGRTVNITPLAIANVPPPAYYRDFDTPENVIDAAVSLSNEIYAVLNKETLIIATVPSLKDMKKGYHPVIASKFSKVDCATEFESFRQIAFINDTVVGILLDTDNFTRVALISIEDVAQPHILKIVDTFDKIVLLRSSFDYNSLVYQTKDGTVMQLDSEGQLEQIARFPQLVRDFRVKRVHNLVGNAIGEWDTDSSEIVAFGLTANGKLYANDVLLASAVTSLEITDSLLLFTTAQHNLQFVHLNSTHFEQLPIVEEGVVDERVRSIERGSILVSVIPSRSAVVLQANRGNLETIYPRIMVLAKVRKDILSKRYGEAFVCCRTHRIHLDILHDYAPELFLENLELFIEQVEKVDHLDFFISCLREEDVAKTVYKETLSAGIAHKFDLEPAPLTEMQQYMKKKMFKPETSKVNKICRAILNVLLSTEEYKKKYKQTIITAYASQNPQNLKDALQLIGAIEDDEERDSLLTYLVFLQDANIVYRIALSLYDINLALAAAQKSQMDPREYLPFLQSLYHNEPLRCRFLVDDHLQNYEKALFHLVKIEKEQNDDSQEIAEEILDYVDNLKLYKHALQLYKYENEKQNRIYHLYAKHLCSKQEYNDAGIIYEMLGDYRSAIDAYTSAKEWRNALSLTIENYPEKLVEVAEQLVSLLSYEHNYSATAEIQVEFLDNIEQGVADYCKAYEFDSACLVASKKGRPELLEKTVDPGLGEGFGTVAELLADCDKQVQSQLKRLRELRSKKETDPYAFYGQEAEETDDVSIAPSETSTKESFFTRYTGKTGGTAKTGASRRTQKNKRREERKRARGKKGTIYEEEYLVKSTGRLIQRLDQTRPDALKLIAGLSRRGMLMQAHQIQRSFADLIQLLKDNLIEIYTLSEKDRERVDDNGEVYYIPEIPIPQIADFPKSKVVDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9U0</accession>
    <name>A0A8J2T9U0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein transport protein SEC31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Protein transport protein sec31</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN860_02234g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPII-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat SEC31 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316462">
        <property type="protein sequence ID" value="CDF90986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T9U0"/>
    <dbReference type="OrthoDB" id="542917at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.940.10">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021614">
        <property type="entry name" value="Sec31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040251">
        <property type="entry name" value="SEC31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009917">
        <property type="entry name" value="SRA1/Sec31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923">
        <property type="entry name" value="SEC31-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923:SF11">
        <property type="entry name" value="SECRETORY 31, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11549">
        <property type="entry name" value="Sec31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07304">
        <property type="entry name" value="SRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47938">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="7 15" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="102"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="251"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="SRA1/Sec31" evidence="17">
        <location>
            <begin position="1127"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="868"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="979"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="996"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1035"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1065"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1094"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1131"/>
            <end position="1140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF07304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CDF90986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1259" mass="135471" checksum="212630B9C32ADC9D" modified="2022-05-25" version="1">MVKLADYSRIATFAWSHDKIPSLVTGTASGTVDADFSSESTLELWSLLSADASKPNASLSVEAKFNDLDWSFDSKTIAGALDNGTIELFSTSEHLLKSEAKFQHHKGSAKVVRFNPKQFNVLASGGSKGEIFIWDVNKCLDSPSDYAPLTPGNSTVPVEEVSSLAWNNSLAHVFASAGSTTYASIWDLKAKKEVIHLTYTSPNTGVKAQLSVVEWHPKNSTRVATATGSDNEPSILVWDLRNANTPLQVLSQGHSKGVLSLDWCHQDENLLLSSGRDNTVVLWNPETGQELTQFPTRSNWVFKTKFAPQAPDIFASASFDNKVQMQTLQNLVNTLDQEQTASKQRESESDFWNHVSQEDPNEKPTLIKLQAPSWYGDKSPAAQWAFGGKLVYITADGKGVTITKPSLPGLEENTTLGEAIKSNNYTPLINQRLAKAMDSTNEEDWSLLEKLSMDGKSEFLKETLAFDDEEEEKEKAGETVDDGAKFFAKIEAAYVPQGDFKLNTEVDQQLGKQLVAANFKSAVVSALDKDLLLEALVIALDSDDKPLKDLVKGAYFAKHGKQSSLSRFLFSISNKSSEDLVEHLDVSQWKYAARTIFSFYSNDAKKRNQELCALGDRLLESGNRQDSLVLYLAANSLDKVANVWLKEFHSNESKVQKSKTSLYEAHSECLTEFVERFTVFSTFVGSETSITNEELVSKFLEYVNLACASGSFDLARSILETLPGNNEDVKMERERVLLASGKSSKSVSTRKPAYGNPHGATNVPVQQQGFGYGAAPAAPNNAFIPPNPITATNYAPVAAAAPVTTTVPKPNPYAPPAANPVASGRYAPVTLSPAHTSFNLPPNPYASTGATSSPYTPMTVKPTPGLVSPPSAPYGNVANAATNPPPPTMKSGQAPHLNKKANDGWNDLPLNVKEKPQRAKAPSVAPINIGAPTANPLAGTSATGTHAAPPPRSGKPSSRAQSMSSPLPPPPPKSSRMASISSNGSIGNISPNAGPLNPPVNPYAPQAQPPSIPNTPRMSAYAAPVAQPPAAPFAYSQTGTTPSPVLRQTNPYAPPPQQVMPVGAIPPSRTSTSAAPAPAAVPPPPTNLKKKNHASASVESASTILEAVQRKPESLSSNGLAHPVTPPVAAPPKSTAPPSTNSLKADPETSAIPDDQQDIVDYLKEELARVTPLTPKEYHKQLKDCDKRLNILFQHLAKQDLLTQPTVDKLRNMVELLKEKSYASAMEVFVDISTNHATEAGNWLTGVKRLIGIAEATDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9W6</accession>
    <name>A0A8J2T9W6_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Structural maintenance of chromosomes protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_01728g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316466">
        <property type="protein sequence ID" value="CDF91706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T9W6"/>
    <dbReference type="OrthoDB" id="10254973at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22879">
        <property type="entry name" value="Smc5_CC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001301">
        <property type="entry name" value="Structural maintenance of chromosomes 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916:SF1">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="11">
        <location>
            <begin position="39"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="196"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="326"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="885"/>
            <end position="912"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF91706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1087" mass="126484" checksum="8333B32422BDC3D3" modified="2022-05-25" version="1">MTLNAANALNLAHYVEQGTKRLKLGTVDHTPFNPGAIIKIRLENFVTYSLAEFDLSPSLNMIIGPNGSGKSTFVCAVCLGLAGKPEYIKRSKRVEEFIKNGEERGSIEITLKNSPVVERIEAVDREDDVIKITRILTKTKSKSEYFINDKPVTESVVKLLISQLNIQLDNLCQFLSQERVEEFARLRSDKLLAETVRSVDSKLINMLELLKELQNKELNVQKELELNKQRFDELTVEKDKLTESVRVYQELEDKKKEIELHSQLLPYVKIKNHKQKLQAYTRDYEEARNNLKSLLKDKKPFNDAKRVFEEKLEEIVHKRDVKDKQLKEDQRDHGKLGEQLETLREDIERKRQQNEYYRNRTKKLESSIAKRKKDLEEKREILKNIQLPSSQLFDELAGQRKELIIKEANLKDAILELDNKASGITHDMRNLEKQMETRLKYLNGNDRIGILDQSPDLREVKNAVLYIRSRPEMRGKILEPPIMSVSAKAPSFAPYLAQCVDYNTSKAFTIVDSDSYEQFSDEILKRYKVNLRELNNDGIRSPVSREDLRNLGFESYLSDFVTGDKRVIEMLCQFCKIHTIPVSRKELTSSQLTRLSNPSKNGSSLFKRIIHGNRVVDFKRSLYGARQFFSIDANIKETPFYQQSVMSEEQKSRIQEEISQLKLKYAAKRQKLDESSKTKSNYKRDLTENSFKSESLAQRVHSLNEVRRNHSLIKTSIESLEVEIRQLLHESKKDVTRKIKHVESEISTQLLNQTKLVREMISHIIKLNQHQKELAEVDIAHVEAQNMDISMAEVLGSFADKEAKLKEDYHNKKKVAKEMRNTEEYRNWMQQIRSYDAETREKLNDRAEEYEKDGNFHLPFIQDIVDRLESEIGMHNHDESSITILSQVERELKQLHKRLPRLVEELKDIKEQIKERRLTLEPKLDEIVSNISKRFSILFKNVGSAGAVNLEKPHLFSEWKIEIMVKFRDNAALKKLDSHTQSGGERAVSTVLYMIALQESTTAPFRVVDEINQGMDSRNERIVHKAMVENACAENTSQYFLITPKLLTELYYHEKMRIHCVMAGSWMPNPTKRSEMVHFGQTSNYVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WU51</accession>
    <name>A0A8J2WU51_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Helicase SWR1</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Helicase swr1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">BN860_03400g</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalytic component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the SWR1 chromatin-remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="372624at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18003">
        <property type="entry name" value="DEXQc_SRCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000005">
        <property type="entry name" value="Photoperiod-independent early flowering 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000655">
        <property type="entry name" value="Protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF1">
        <property type="entry name" value="HELICASE SRCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00573">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSA" evidence="22">
        <location>
            <begin position="299"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="20">
        <location>
            <begin position="682"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="21">
        <location>
            <begin position="1226"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="125"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="438"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1444"/>
            <end position="1466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="142"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="451"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="463"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="506"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="536"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="594"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CDF87305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1491" mass="170360" checksum="1E223C5A29720677" modified="2022-05-25" version="1">MTKDNGKSKQQELEERSSLAQLKFEYDLLANELFHLKEYISLVEFDPSYVNDSESYHRFIQDQALSLENLQPHTSNQHNIDAPVRRRNLRTAAAGSGSNIGSESIENWLQEVKPLVRDKYSQLQTQLSTGKGIKRSAISRPKAVKKPKPQVKNSKKHEEYDEAEDADDMQQSNFDGDSDNYYFTSSSEEEGESRRPPRKRRARINLVVHPPKQTVTNPLHVIKPHFKNLHSYMESFKSMDEDISIEEYEEYIKEQRKIVKAIRGGLESGALKYDPDTDSIQPITTKEARLIQSHRPDPITYFYKEQNLQTYQEYLLNHGVYMSKLFQNSKKARIARARKVSQMIEQYFKHIAGAEERKAKEEEKRKRGLARIAMQAVKKRWTLAERAYKILRNDEEEKLKRIKGKEHLSKMLEQSTQLLGAQLNQNIEDNAISDKDAIDHTASDSSNNTDNSDDSDDEDDDNISSSSSQDESGSVSSEDEERSSLAINEQLKVGNDVSATDDHQLNTHNANSNPEFSSPEKISTTESTEEHHVFTESEGSSVSTEPSSSDELSSDDNEPLEGNGVGLSSLFDADLADEDDNKSEDFTMPSDSESGEHNVKKETTETASNGDEVQVAEKLQSHNVKHPEEKMEEGPKVEQKANNVLDKAVLDADPPSVIDVAVPFLLRGTLRIYQKQGLNWMASLYNNNTNGILADEMGLGKTIQTISLLAYLACEKKNWGPHLIVVPTSVLLNWEMEFKRFAPGFKVLTYYGSPQQRKEKRKGWNRPDAFHVCIVSYQLVVQDQNSFKRKKWSYMILDEAHNIKNFRSTRWQALLNFNTERRLLLTGTPLQNNIAELWSLLYFLMPQTITDGAGFSGFADLDAFQQWFGRPVDKLIETGEGYSQDAETKKTVSKLHQVLRPYLLRRLKSDVEKQMPAKYEHVVYCRLSKRQRFLYDDFMSRAQTKATLASGNFMSIVNCLMQLRKVCNHPDLFEVRPILTSFCMDQCVARSYTDLNKFVLKLTQQGQQDNSVDLGFLNLAFSSNDSGMTTHRSQSVNQLQCVDEFVKQVRLLRERSLLNPADQSTSTENPNFHSAEQFFRRLGQQKSQRAIDSLEFLRYINELKCGKKPVYGQNLIKLLQISRCNSNPTDSPRCMNNLLKPLQNHIKVHSNIIENYAVVTPNAVALDLRELTLGLDDASSFDEHSRMQLLGELQSMPNPLHHLQTKLTIAFPDKSLLQYDCGKLQKLAALLQDLKDHGHRALIFTQMTKVLDVLEQFLNYHGYLYMRLDGATKIEDRQVLTERFNTDPRITVFILSSRSGGLGINLTGADTVIFYDSDWNPAMDKQCQDRCHRIGQTRDVHIYRFVSDHTIESNILKKANQKRQLDNVVIQEGDFTTDYFTKFSVKELLGSEIPASLPVGDKPLLQEAPGVHKDPRRLERLLAQAEDEDDVKAANLAMKEVNVDEEDFADNPNKDHNAAPEDELDQYDEYEGTNHVEEYMIRFIANGYYYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WUY5</accession>
    <name>A0A8J2WUY5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BN860_10418g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_10418g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WUY5"/>
    <dbReference type="OrthoDB" id="241990at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031567">
        <property type="entry name" value="CRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008828">
        <property type="entry name" value="Sin1/Avo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031313">
        <property type="entry name" value="Sin1_PH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056385">
        <property type="entry name" value="UBL_AVO1/Sin1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335:SF1">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16978">
        <property type="entry name" value="CRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16979">
        <property type="entry name" value="SIN1_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23164">
        <property type="entry name" value="UBL_AVO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIM" evidence="3">
        <location>
            <begin position="607"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="domain" description="AVO1/Sin1 ubiquitin-like" evidence="5">
        <location>
            <begin position="807"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="domain" description="SIN1-type PH" evidence="4">
        <location>
            <begin position="1023"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="87"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="160"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="439"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="510"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="934"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="160"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="234"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="330"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="402"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="521"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="540"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="551"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="944"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="960"/>
            <end position="970"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF87613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1133" mass="127599" checksum="1A1E0165D909BF53" modified="2022-05-25" version="1">MDTETSINRLRAQFLAECPDKDLMRRIIRPYCPGHDILQPKDVDLSIRKLYVTPEGLDIFPDLDSPPMSRNYMDSYVSTTRKALDKSKALSKSHENSGSEVDIENYKKEHENEREKQLEIEKTMTVTRDSPELSENILPMPMLVESNKEIKEGQDDMIETSISDSDSDSDSTSTSKGSRKRNNRKLPFSRIFKGKKENPDTTRKSTNKKKPSISSQGITKHSAFDINFGYDHGLEEDDEDEDEDEDDDGSDLHSQFFQLDAEASKTSNSNEAAENELQPNKPSSSNSLSYINNNSSNTNVAGKDAPKKKSHFPQNMIPRPTLLEGGKNSSKSEKMLKAGGHDDYLGTSAAPLEGNTSEGQAEDISDIESYINEQDLDNLNLDATPPAHSKSINSKNELGMSSHKDNENSRSLEESGNMIEDRKNSDVVSSYGKSLLDSDFSGDNVFKPGESALDDNSMLDDSDLQPDTVSHSIPLSLDDYGIYHGQDDTTLDTAFDKAVLNIKGLKPLHGKERRSFGRPVSRSASKYSNSSNAKQREHLNSLTTASGESSKVLKHEDSRPQLHGISDSKSERPITPASPIPNSKSHSKSDWLTIEKTTDFDKPKPHNSQLSSLFKKKINPSESSKDLLEYFSFISGNKVPRPEATELNVYIQASKNFRKKPFKLKVRKSANIFEVIGFILYHYAVELEEKNLKDDGLKKEELENPNRFCLKIVDEDGAPFEDNFGKLDRKKLIQTLSDNEVVLCEVSDEEMSENERTTPLPFIGGNEVIDGVESNDKQVNPGRINQLSYYRPIVGQNDAKDVAGDSKSIEIKVYIYPNTNKKFNYTNINVSVTSSVNDILVRYCKMKHMDPNEYLLKIPDKKLALNLNDTILRLDGHYEVEIISKREARELGLEKLKPDTKKPNLPTIQSDDLTPLTLEPAAGYLKPEKIEKVEKDNQNTGHKPLSKKKLSAKHKLGIANQSSTSSSNNSVPAGNGFFKSKNNSKTSLHGSLSYYAPNRPLLAASGVAAGLTDNGYQDLFSGAYHRYRVWRRQQMSLMNKHERTLALDGDYIYIVPPENYLHWHENVKTKSLHISQVILCKKSKRVPEYFKIFFRRGQDEIKRYYFEAVSPEECTEIVSRIQNLVSAYRMNHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WV69</accession>
    <name>A0A8J2WV69_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_13058g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_13058g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WV69"/>
    <dbReference type="OrthoDB" id="1938992at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033255">
        <property type="term" value="C:SAS acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038988">
        <property type="entry name" value="Sas4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029184">
        <property type="entry name" value="Sas4_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38422">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38422:SF1">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15460">
        <property type="entry name" value="SAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Something about silencing protein 4" evidence="1">
        <location>
            <begin position="84"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF15460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="368" mass="42774" checksum="B5DF89744D53E41F" modified="2022-05-25" version="1">MPQDTRSLRSKEGAKIKDEEKFNFDDEEYEINPTKKLKTVSRGYPKDTLGHETLLSANEKIKLIDSMALKLEPHAITLNGSKEDALSDDLYLAYHKKMSRHEIRMLDSDLIQGENEADRLLLLSEKLDLAQWQTTLKKITVIRNPDDENEMERKRLLTKETIDSMLKTYHTMKKNRTIALKNHRSGKIDPVKHWPQIYNSIDRRVVLGYHSSSDEEEIGMNVDEIKSHRKMKREKQCRGSIIIQLTMAPHSYQTRYAIVAEPLRKPYVIKISNEERQKWNKQMQDAPEKFIHYTELPSQIAVPKRKVSIPFTLNQQPVKHEKSGSEQPSFHVNASFSKRQTLAPKTVDSANGLRVEARILPVKRIKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WXK3</accession>
    <name>A0A8J2WXK3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Exportin-T</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Exportin(tRNA)</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA exportin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_15962g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">tRNA nucleus export receptor which facilitates tRNA translocation across the nuclear pore complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
        <text evidence="9">Shuttles between the nucleus and the cytoplasm.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WXK3"/>
    <dbReference type="OrthoDB" id="26399at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071528">
        <property type="term" value="P:tRNA re-export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045546">
        <property type="entry name" value="Exportin-T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040017">
        <property type="entry name" value="XPOT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15952:SF11">
        <property type="entry name" value="EXPORTIN-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15952">
        <property type="entry name" value="EXPORTIN-T/LOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19282">
        <property type="entry name" value="Exportin-T"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <keyword evidence="6 9" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Exportin-1/Importin-beta-like" evidence="10">
        <location>
            <begin position="96"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Exportin-T C-terminal" evidence="11">
        <location>
            <begin position="336"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="domain" description="Exportin-T C-terminal" evidence="11">
        <location>
            <begin position="859"/>
            <end position="1057"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF19282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF88682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1059" mass="119196" checksum="766C56D5D856208F" modified="2022-05-25" version="1">MLQRIQEVVSIANSASTDAITKKQALDFLSQIKAEPNAARIFASLLGDSQCDDLTRFVALQVLCDLALQAENDLQFIRTTTVELLRHKVVEDSRDPEYVRNKIAELVTNLFYSMYGEINGNQWNSFFADMAEILSIKGLLNETTKNFAPSGLDYFLRICLSINSSIADQTFVKSKDMQLKNNSLKDTMRLQDVQTLVSLWLNTLKSLIPQEQSGLAVLTLSCVGSYISWIDVQLIVNPIYITVIYGYLDFENTKIACAQCLCEIISKKMKPLDKLSLLSMLSLTDKVANVGRDDLEVHEHLAKLPASVGLELTKIIEQCNENGSMLDLQQVAIAADEQIINQVAPLTLKFMEHEYDSVTQQCFPFISQYLSVLKKQFAIGGKPGSAIAINSKKLPLDPAHEMFLTSLLLVGFKKMRIDDSSDEDAEDDIDEFFDTIRSKLKVFQDGIAVINPPLYLQNISNHIQDCLSKMDWRDLELGVYQMHNLSESIRNNLFGLAKHEIANSEASQLMAKFMSVLLQLSTVFQMDNPYVQILFFELVVRHYTFLGSDEKDELALLNIFCSPFGMFNKREKVRLRTWYLFTRLIKTTKPKLSTPVLSQLLAKISPLLVIKAVTSDQDGTDYDTTFDNQLYLFEGIGLLIGANSNLNYDILDDVLSPLFAGLETCISSQIQCPEVILQSHHLLMAIGTLARGVHGGLVPDNQVNNALVSEKLIHRTLIEKFSNIAEVVLVSFSYFNKHENVRDASRFTFARLIPILNNQVVPFASKFIALFLESELGTLEMNDFLGFLGQMIHTFHADDSCYELFDNLLTPVIEKVHKVMDHIDQESARETETWYNGNSSGTVLAASGKDPNVKNVVVTDSFRDKIQLKKAYYGLLQSFVTNSVTSLLLTERNRATLSTILMDLLVYVPQEVQETSTIKLALNVLCNFVRCFGSGKCIDPNDRYAANIGQLEGLNEFFITKVVPLVFEIPFKPEYKFNIRDGSCRVVACDLSRLLKELYVQCGGGTDPLANPCLKYMGQVYFPQIQFPSELGLELIQMLAMADSKAFEKYYVNLINNLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X4G2</accession>
    <name>A0A8J2X4G2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Coatomer subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_03312g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316463">
        <property type="protein sequence ID" value="CDF91261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X4G2"/>
    <dbReference type="OrthoDB" id="10261470at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22948">
        <property type="entry name" value="Coatomer_WDAD_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000002">
        <property type="entry name" value="Coatomer subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000022">
        <property type="entry name" value="Coatomer subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047312">
        <property type="entry name" value="Coatomer_alpha_WD-assoc_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016391">
        <property type="entry name" value="Coatomer_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010714">
        <property type="entry name" value="Coatomer_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF1">
        <property type="entry name" value="COATOMER SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06957">
        <property type="entry name" value="COPI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003354">
        <property type="entry name" value="Coatomer_alpha_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <keyword evidence="4 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="49"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="91"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="133"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="205"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="249"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B second beta-propeller" evidence="13">
        <location>
            <begin position="343"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="15">
        <location>
            <begin position="627"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer alpha subunit C-terminal" evidence="14">
        <location>
            <begin position="845"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="840"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="857"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR003354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF06957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF91261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1199" mass="134647" checksum="EFABF19B024D8B62" modified="2022-05-25" version="1">MKMLTKFESKSTRAKGIAFHSSRPWVLVALFSSTIQLWDYRMGTLLHRYEDHEGPVRAVDFHPTQPLFVSAGDDYTIKVWSLETNKCLYTLTGHLDYVRTVFFHTELPWIISASDDQTVRIWNWQNRKEIACLTGHNHFVMSAQFHPSEDLVVSASLDETIRVWDISGLRKRHSAPGTTSLEDQMIMQQNLLDGGFGDCVVKFILEGHTRGVNWASFHPTLPLIVSGGDDRQVKLWRMSSTKAWEVDTCRGHTNNVDCVIFHPHQNLIISVGEDKTLRVWDLDKRTPVKQFKRENDRFWLIAAHPHINLFGAAHDSGIMVFKLDRERPCSFIHQNELVYVNKEKQVQTFDYSNNVSSLPYASLKKLGQPWNSFKSISYNPSQHSILVNEGNDRFALVLLPKRPTGAVEPSGVLEDAGCFATFVARNRFVVYNRMAESLELRTLENKVTKTLKLEEPVKDIVNAGAGSVLLLQPKQVVLFDVQQGKKLAEIALKNVKYVSWSLDGQYAALMSKHTITITTKRLDLINSMHETIRIKSACWDETGVLIYSTLNHIRYILLNGDNGIIKTLENTLYINKVKGKFIYALDREGEMEILTIDPTEYRFKKALANKNFPEVLRIIRNSNLVGQNIISYLQKSGYPEIALQFVQDPQTRFDLALECGNLDVALEEAKKLKSNLVWDRLSKEALLQGNATLVEMVYQTQMAFDKLSFLYLLTGETNKLAKMETISQNRGDIFDLISDTFYNDSTTTRANAFAEAGSLPLAYAVAKATGNESAAATYLEQDEVDENSVILPDVVNSTSAIKAPAVSQPIDKWPLKKAELSFFEKAVLNQIDDLNLDEPAEEEDTVDHPKTQQSEAVFEEDGAGEEGDAWGSEDLDIGEELEVEQPTKETTAPAEESETAIWVKNSKLPAVLVASGAFDAAAQALNKQVGIVNFEPLRSKFLDIFDGCRTYMASTPSEVPSIVGYVRADPEEEDPTKILPRTPDISTVTSKMNEGFKYFKANKLEPAIECFRDAIYIITLLAVNNEEDETTARQALQKAKEYILGLSIELTRRSLPAEDVKRNLELAAYFTRAKLSPAHRSNALQVAMSQSFKHKNFVLASYFADEFLKIMSSGPRAEQAAKVKDKADSLAHDAVEIDFDPYAEFDICASSYTPIYKGTPSATDPLTGAKYHISEEGKIDKIALISKIGARSSGLRIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X575</accession>
    <name>A0A8J2X575_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BN860_08438g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_08438g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X575"/>
    <dbReference type="OrthoDB" id="3364175at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF3">
        <property type="entry name" value="HALOTOLERANCE PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="16"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="895"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="948"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="952"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="973"/>
            <end position="982"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF87528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1035" mass="117837" checksum="AC1951680C9CB32A" modified="2022-05-25" version="1">MESGRVKKQRSKVNRACDNCRRRKIKCTGKFPCSNCEAYQCQCVYSSGQFPANNNTRVQHSDPNERPFPPSEDGVVNMSSLGSSGRTDKPCPKISLDNYQLESNRCVHTQKSSSSPALDLNALPLIELNTDEKGLYKSDVEFQKQLLNLQKALKHLNAVPNRNEHVAEVINNINTQVDNLIRDWEPEYDVKEYGKFLENGSEGVKSIETHLMQNKYTDQVTLTAFATWTDNNKAEKGNLPGSSFLLNQPLVDDVFGLYSPLQAMSLRGIGHLFQRCIKKATSSEEIVQVKENLYLLLRFFDICMDQFNQSCVSIANPLESYLQRKNALALSPNTTSSVSSRNSANNKDLVRVLINRLPQPFVQNVTSVSNKQLLDTMQDDFDMFSLVLKMYDDHKKGFESLMIKITANPDDSVMITPRLSTADIQDFICYCEEEELLLALCYSYYNSTLYHFDEFSRSLEYFELLLSLLDKQHWLEEEYGFHKVLDVAINYAVGMGLSRWEFYVGLDEETAERRRECWWKLYCIEKQYTFLTGYLSSIFDSKMNCLLPKVFRDAGFSDHRDFVAKVHLVDETSVFDAMSPADLAFYGECAICQVTSNFYWDTLYNERFTSIKNSAKPPYVRYCLLEEVLRNIDLLKLKLDAIRKQTEKLSRIAYSNDSIADDTASKQAKALAVRHVLLQGCLLFYVSSAVSNLVARLQVHPNQANVKASEIKYSGILHQEFIEMTKLLLSLDNDYSVSRAFVMYGHVYLMVVTGSFSGCCIEPTVDEVVMSLRLVRRLRDVYIYRENDDNEIVRGSKVYNDFRRLGSFVSVITNSLLQSFMLHNDLTKDELIKTFANAAPDVADMLPLILNPKSDIYRHLMEPVQESGVHLNVKKMLGKIQKYPIQRRGYPRTAVQFTSPSSKNPQHLNPYNSPQQDVALPQTSLNSSNNLAPSVKNLTNPMVMPVDGFLTPGNSNPIAESQDPNLRQEKQTHVPSISNEDSNKFNLGTLDEFVHKGDLNDLYSTLWSDLYSDELGQSLSAFSNNYTSGFDTTRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X586</accession>
    <name>A0A8J2X586_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S13-02586g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_02586g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316466">
        <property type="protein sequence ID" value="CDF91745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X586"/>
    <dbReference type="OrthoDB" id="775356at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05137">
        <property type="entry name" value="RasGAP_CLA2_BUD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF60">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN RASKOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="299"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="4">
        <location>
            <begin position="478"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="968"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="994"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1005"/>
            <end position="1014"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF91745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1014" mass="116020" checksum="CCA63A700614511A" modified="2022-05-25" version="1">MSNSSITTSFSGTRFDLDKFLSTMQSLKGTFQGTLNWSYDLHSNSWKTRAVEITDNGVLRCATDSNEDMGKDNGSVLIDHLQSCKVRFLDEHSSGFPTICVSAHSKRMFLRVSDAATFEELICAFIWWSGLRKNGIFQKFTPERPLLTSDFDATKVLVSQLYVFGPVLNKTVPIDKNMIRPDFLPDSELEEGWFPAMGVLKSNGVLDLLLQQDGTLIYSLNIAALLRSEIRIPDPSLDSDNYLFLNIIPTLREQLKISSSNKFVGTPKHVPSPAIILKFPLKIDVEDWFVALKSFAIKENFSLIGSDKSNHVRVSNSFSISILEGQFKQPKHEEELPCLYVELSMWGYVWGKAPVVEHTCAPFWREVFTFHESLKVNSLRIKIKRKSHNKTKDEMIGYIDLSQENINDPKLSKETRLPVLSATKRNEQIGTLCIKIVSSLILVLPSSNFIRMEKLLAELNLEDLCDLIQDFSGGIDVKIDNVSEIFLDIFLAIGRKDAWFDALLKKELSAVDDSIARNTIQNKPSSHIYSTLFRGNSILTISMEKYAYRVGHEYLDKSIGNILRDIINQQVSCELDPARIDERNPEMVEKILSKNRNTLRHWVGLLWDRIYRTSNDLPMGIKEQMKMFRKKLELICIEEDFNTTLNCVSGVLFLRFFCPVILNPKLFNLVSHHLNETSRRTLTLICKVLLNLSNITFFGNKEPFMIGMNDFIECHKDDLVDYIDKVTLKKLDFSPRILKLTSSVARLDLTMNEEELKELPVNPYLIDKYLRETEIFTFLASLTLKVENELSTDKKDETGDNEVIYDRPVMGQKLMIRGLEFEKITENNTEIFGNDLMKYLEEDELLTPDDTKPKRDFSNLNADVMGQLKQESLLLYHKIGNLTQMLSDFEYPSEVVLSQPNYSEYLADNTYYTKDKKLKTNLLGDLGDIRGLKKLFDFETKTSVLFDNVVPRSSSIIVERPVSTTSASESSSTISRNLTRLRSKRLPKIMSGNSEDKQSKEKSPGKLKRLFKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X596</accession>
    <name>A0A8J2X596_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_12156g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_12156g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X596"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035286955" evidence="2">
        <location>
            <begin position="18"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="196"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="243" mass="23761" checksum="95F2E8466DE53514" modified="2022-05-25" version="1">MLYQVALSFALASTALAEVSGSITYVDETTTPQVTESVVSTEDVTSTPYITTTLSSCTDSSESAAESTAEATGYTAASTPVSTPLSTAASTLTSAPASGSAAETAQQTATSETAVTEETTATAGQDTTTTIHPTDVTSVSCNETSANGTTVFSSSTSVVPTAFSSTSASSSAYANDTTSASGPSTPLSSGSIVPQSTSASVSPSGSAEDTASSSTAQLETFTAGGSRHVASFGALAAALVALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5D9</accession>
    <name>A0A8J2X5D9_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">T-complex protein 1 subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">CCT-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_13982g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5D9"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03335">
        <property type="entry name" value="TCP1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000009">
        <property type="entry name" value="T-complex protein 1 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000070">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012715">
        <property type="entry name" value="Chap_CCT_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02340">
        <property type="entry name" value="chap_CCT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF87765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="559" mass="60312" checksum="A02EE56640014E2A" modified="2022-05-25" version="1">MSQVFNSSRSDTLFLGGEKISGDDIRNQNVLAASAVANVVKSSLGPVGLDKMLVDDIGDFTVTNDGATILSLLDVQHPAGKILVELAQQQDREIGDGTTSVVIVASELLRRANELVKNKIHPTTIITGFRVALREAIRFINEVLSINVDSLGKSTLVNIAKTSMSSKIIGADSEFFSNMVVDALLAVKTQNSKNETKYPVKAVNILKAHGKSAKESVLVHGYALNCTVAAQGCPKRVGGGNVKVACLDLNLQKARMAMGVQINIDDPEQLEQIRRREAGIVLDRVKKIIDSGAQVVLTTKGIDDLCLKEFVEANIMAVRRCKKEDLRRIARATGATLIGSMSNLEGDETFESSSLGTCQEVVQTKFSDDECILIKGTSKHSSSSIVLRGPNDYSLDEMERSLHDSLSVVKRTLESGNVVPGGGCVETALNIYLDNFATTVGSREQLAIAEFAQALIVIPKTLAVNASKDSSELIAKLRAYHAAGQQAKHDDVKRRSYRNYGLDLIKGKIVDEVQAGVLEPTISKVKSLKSALEACVAILRIDTMITVDPEPPKEDPHGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5I3</accession>
    <name>A0A8J2X5I3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Spindle assembly checkpoint component MAD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mitotic arrest deficient protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_16446g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5I3"/>
    <dbReference type="OrthoDB" id="331602at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008672">
        <property type="entry name" value="Mad1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23168:SF0">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23168">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD1 MITOTIC ARREST DEFICIENT-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05557">
        <property type="entry name" value="MAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="69"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="219"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="315"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="535"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF87874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="652" mass="76660" checksum="64F9639BA5914A37" modified="2022-05-25" version="1">MPGSSSGSSPFLESPVHAIPQNNQSTDRQVMSLQYKLNTLQNQFEIEKLRLQQQANLADKKYRNTVDELEKALDDTKYLYENNNRLEEELANLKEKLGDGGSVKDQEYQKLQADLSSKDYQLERMKSEYESRLSNLENKLETSEMEAQGSQTLLRKYEDEIARKTDEVKCLQKANIEKDDEVTTLRASRVVMEHHNYSTEEFQELTTTNKMLQDQLQYSKELEETNMQQALELKRLRMANESQLFWKNENEKLQNKLEQLNTLERQFQDSQLENVNLKSQIASWGLYTDEKHSPEHIIRDWTISKKECLVLTDENEKLRLDLMNVKALNDELAQERNQLLDANRNYEANILNLKKLNHEVEQQKQLSFEECKLLRAEMEELSKFSKDSQKEDVAIYGSLVDSYKNQTEDLTGELKKLNDQLLAREPVYKKRKASDQLGLNYSQRLNELQLQNLALSRELQKCQSTIGILEEKIIKLTELKEKKIRILQLRDNPLLKDQFVKRKQLELLKRENEDLLLLRQSSGRVDFETVPVAVYDTMKFELKQYEEELFKANKKFMRLKEVFNNKSLEFIDVVNSILGFRLEFQQDGKVKIISCFKPEKYLVVDLNQNTLKSNLDRDIDGWEELLQIWVDQRGQIPCFLATITLKLWNSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5S2</accession>
    <name>A0A8J2X5S2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BN860_18668g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_18668g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5S2"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF15">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="19"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="638"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="388"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="503"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="647"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="109"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF87970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="660" mass="73333" checksum="22FD6E10102941C1" modified="2022-05-25" version="1">MAIQSVSGAYTGPIAQPSPMRVLLRLKPYSGEAAKLSYVEDGSGAISMQDFPQVGGKTTFFTEGNDTNKFKFNKIYGVDVMQHDVCDDIIGDNVEELLNGFNITIMAFGQAGTGKTYTMFGNDHEKGVVPKICQALSDQLEAAHKDGTEVTITLTFFELFAESVHDLLAYPKTVKSLKTSSDPKTQSISIKGLRNVVVTSAKELQKYVEAGQGRRNSSDSQCKNSRSSAFIRITLEQRSRKQEIVKTSMLQLVDLASSDKLDKNAKVDISSDELKAINSSMQNLHHLVHSLADMKVAERDASATSKTSHAPHRSSRLTKLLQDAIGGNSKTTAVILCSTDKDDKEETVSSLNFGVKLKLIDNVIEQNKEGLNSKAVMDLAMKNMRIREDSYLTRIKYLEDEVKKLENLLEQNRSDVLMNRSLVEENAKLKDEVNLIRKSKENSAYQGQVQPGKDGEAQYDSSEILENLMEKCEKVIQLQLRLDEELHQKSSLSQQLRYKGSKAQALEAMNVKLLEQLNAYEKELKSVSNANSLLRQDVEKWSNVAAARSEKIEELEKEVQEHQLGTFPQKTRLVSAGELNHSKDDSKLKKSNWLFGNSNGNIWSTRKVSTVSTSSAITTNSQDSSSSRTSLRGLNLHAIRLPPSGENENHADNEKEAKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5X5</accession>
    <name>A0A8J2X5X5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dolichyl-diphosphooligosaccharide--protein glycotransferase</fullName>
            <ecNumber evidence="6">2.4.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">BN860_08812g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a di-trans,poly-cis-dolichyl diphosphooligosaccharide + L-asparaginyl-[protein] = N(4)-(oligosaccharide-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl)-L-asparaginyl-[protein] + a di-trans,poly-cis-dolichyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22980"/>
            <dbReference type="Rhea" id="RHEA-COMP:12804"/>
            <dbReference type="Rhea" id="RHEA-COMP:12805"/>
            <dbReference type="Rhea" id="RHEA-COMP:19506"/>
            <dbReference type="Rhea" id="RHEA-COMP:19509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:50347"/>
            <dbReference type="ChEBI" id="CHEBI:57497"/>
            <dbReference type="ChEBI" id="CHEBI:57570"/>
            <dbReference type="ChEBI" id="CHEBI:132529"/>
            <dbReference type="EC" id="2.4.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endomembrane system</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.18" evidence="6"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5X5"/>
    <dbReference type="OrthoDB" id="10261066at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004579">
        <property type="term" value="F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043687">
        <property type="term" value="P:post-translational protein modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048999">
        <property type="entry name" value="STT3-PglB_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048307">
        <property type="entry name" value="STT3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872:SF1">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21436">
        <property type="entry name" value="STT3-PglB_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="183"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="281"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="311"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="372"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="400"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="463"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyl transferase STT3 N-terminal" evidence="18">
        <location>
            <begin position="31"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="STT3/PglB/AglB core" evidence="19">
        <location>
            <begin position="527"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="703"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="703"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF21436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CDF87544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="733" mass="82931" checksum="FD3ABAC53CE2B5B7" modified="2022-05-25" version="1">MKASSSTPAGEGGPLLHASPNALVKWIQTILTFAIFVAIFGAAVSSRLFSVIRFESIIHEFDPWFNFRATKYLVTHSFLEFLNWFDDRTWYPLGRVTGGTLYPGLMTTSGAVWHLLRRVGLPVDIRNVCVMFAPAFSGVTVLATYQFTKEIKDGSAGLLAAAFMAIVPGYISRSVAGSYDNEAIAITLLMVTFMFWIKAQKTGSILYATLTALFYFYMVSAWGGYVFITNLIPLHIFVLILMGRYSSRLYSAYTTWYTIGTLASMQIPFVGFLPIRSNDHMAALGVFGLIQIVAFGHYVKTKVSSDKFRVVMAVSLVLILAVGVSALFALTYFGYIAPWTGRFYSLWDTNYAKIHIPIIASVSEHQPVAWPAFFFDTHFLIWMFPAGVFLLFLELKNEHIFVIAYSVLCSYFAGVMIRLMLTLTPVICVSAAVALSKVFDVYLDFSSTVKDRKVNFGNLLSRLFVSSTFFFYMVLFVLHCTWVTKIAYSAPSVVLPSTSYDGRTILIDDFREAYYWLRVNTPEDAKIASWWDYGYQIGGMADRTTLVDNNTWNNTHIALVGKAMASPEWKSYEILKQLDADYVLVIFGGLLGFSGDDVNKFLWMVRISEGIWPDEINERNYFSSSGEYRVDSGASQTMKESLMYKMSYKDFPELYGGQGVDKVRGQTILPEDVEPLDYFEEVFTSENWLVRLYQLKKDDPEGRSLKEAGEFERNSAKSNKKKSFRKPDLDLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6B5</accession>
    <name>A0A8J2X6B5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">Homoaconitase, mitochondrial</fullName>
            <ecNumber evidence="5 17">4.2.1.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 17">Homoaconitate hydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">BN860_14730g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 17">Catalyzes the reversible hydration of cis-homoaconitate to (2R,3S)-homoisocitrate, a step in the alpha-aminoadipate pathway for lysine biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 17">
            <text>(2R,3S)-homoisocitrate = cis-homoaconitate + H2O</text>
            <dbReference type="Rhea" id="RHEA:15485"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <dbReference type="ChEBI" id="CHEBI:58174"/>
            <dbReference type="EC" id="4.2.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="17">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 3/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.36" evidence="5 17"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6B5"/>
    <dbReference type="OrthoDB" id="10262323at2759"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER01027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004409">
        <property type="term" value="F:homoaconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01674">
        <property type="entry name" value="Homoaconitase_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004418">
        <property type="entry name" value="Homoaconitase_mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039386">
        <property type="entry name" value="Homoaconitase_swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050067">
        <property type="entry name" value="IPM_dehydratase_rel_enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00139">
        <property type="entry name" value="h_aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822:SF2">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 17" id="KW-0408">Iron</keyword>
    <keyword evidence="11 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="14 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="13 17" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="19">
        <location>
            <begin position="22"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="20">
        <location>
            <begin position="491"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="480"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CDF87798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="696" mass="75333" checksum="25D0FB37A083643D" modified="2022-05-25" version="1">MLRLCRRLHSTPRRNAQNLTEKIVQRYAVGIPKGKEVHMGDYVSIQPEHCMSHDNSWPVALKFMGLGASKIHNPRQIVNTLDHDIQNHDKKNLTKYKNIENFAKSHGVDFYPAGRGIGHQIMVEEGYAFPLNLTVASDSHSNTYGGVGALGTPMVRTDAAAIWATGQTWWQIPPVARVELRGALPQGISGKDVIVALCGVFNNDEVLNHAIEFTGDLANLPIENRLTIANMTTEWGALSGLFPVDKTLLQWYREKRLPRMPKDHPRINNARLELLERALENDDASLRSDSGASYAKKLVLDLSSLTHYVSGPNSVKKSAPVQDLTNVKVDKAYLVSCTNSRLSDLEEAAAVVCPSGDAIEVKQVAPGVEFYVAAASSEVQADAKRTGAWDKLLRAGCIVLPPGCGPCIGLGAGLLEPGEVGISATNRNFKGRMGSREALAYLASPAVVAASAVLGHIASPAHVTGHADATFKGVKAFEENETQKEAPAEEATSTVPESGTEILEGFPSKISGELVFCDADNVNTDGIYPGKYTYQDDVPREVMAKVCMENYDAAFRDIAKPGDILVSGYNFGTGSSREQAATALLAKGIPLVVSGSFGNIFFRNSINNALLTLEIPDLVSLLREKFGKEAAKPTRRTNFFLTWDVAHRKVVVTEGSPDGKVVLEQQVGELGKNLQEIIVRGGLEGWVKTKLAGDSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6B9</accession>
    <name>A0A8J2X6B9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">BN860_06788g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_06788g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat TAF5 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6B9"/>
    <dbReference type="OrthoDB" id="10266330at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08044">
        <property type="entry name" value="TAF5_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.500">
        <property type="entry name" value="TFIID subunit TAF5, NTD2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007582">
        <property type="entry name" value="TFIID_NTD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037264">
        <property type="entry name" value="TFIID_NTD2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF1">
        <property type="entry name" value="CANNONBALL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04494">
        <property type="entry name" value="TFIID_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160897">
        <property type="entry name" value="Taf5 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="TFIID subunit TAF5 NTD2" evidence="10">
        <location>
            <begin position="150"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="508"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="550"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="592"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="634"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="682"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="87"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="352"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="19"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF88298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="785" mass="88041" checksum="C15B328D9212E3A2" modified="2022-05-25" version="1">MAQNGNQKRPAASSNGAAQQQQPQQQQQQQSQQPQQQPQQQQQSQQQQQQRAQPRPSGPFSASDLNRIVLEYLNKKGYHRTETMLRAESGRTLTSPNKQSPANVKTGKLPEPKTTPAALDKNARPVSNPSIPVKRDSSVVSEEQLKETTAPENYIRAYSMLKNWVDASLEIYKPELQYVLYPIFIYIFLILVSKNAVHARRFFDRFSPDFKLNHSSEINRLFSVNSVDHIKENEVANAFHTNRYRVSITRTTLNLLLYFLNENEGVGGSLIISAINQHLDPNIVDTVTSKDQLADGIKMLEKGNSTDTHINTAPIKLGPYPRDEEFSKEIETELKIRDDQEKRSDKHTLVQEYKAMNNEPYDVPEPEVKEEEGEKKEDDEASLPKAEEKKPTTKPAAQPEDANMESPALDAIPLPPKTTLDIKLEVQKVKESRDAIKLDNSQLALPSVCMYTFHNTNRDMTCLEFSDDCRLAAAGFQDSQIKLFSLDGSPLSNKYDPELADDATSKTLIGHSGTVYSTDFSPDNRYLVSGSEDKTVRLWSLDTHSALVSYKGHNHPVWDVKFSPLGHYFATASHDHTARLWSCDHIYPLRIFAGHLNDVDCVSFHPNGCYVFTGSSDKTCRMWDIATGDSVRLFLGLTAPVISTCVSPDGRWLSTGSEDGIINVWDIGTGKRLKQMRGHGKNAVYSLAYCKEGNTLISGGADQSVRVWDLKKATTESGPEPEQPFVGYLGDVTTSINQDIKEYGRRRTVIPTNDLVTSFYTKKTPVFKVKFTRNNLALAGGAYRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6E0</accession>
    <name>A0A8J2X6E0_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">nitric oxide dioxygenase</fullName>
            <ecNumber evidence="4">1.14.12.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BN860_15390g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 nitric oxide + NADH + 2 O2 = 2 nitrate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19469"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.14.12.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2 nitric oxide + NADPH + 2 O2 = 2 nitrate + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19465"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.12.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.12.17" evidence="4"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6E0"/>
    <dbReference type="OrthoDB" id="436496at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008941">
        <property type="term" value="F:nitric oxide dioxygenase NAD(P)H activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071500">
        <property type="term" value="P:cellular response to nitrosative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046210">
        <property type="term" value="P:nitric oxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06184">
        <property type="entry name" value="flavohem_like_fad_nad_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14777">
        <property type="entry name" value="Yhb1-globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.490.10:FF:000003">
        <property type="entry name" value="Flavohemoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396">
        <property type="entry name" value="FLAVOHEMOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396:SF3">
        <property type="entry name" value="FLAVOHEMOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0216">Detoxification</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Globin" evidence="15">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="16">
        <location>
            <begin position="147"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CDF87828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="396" mass="44415" checksum="54DBBC213ACF1238" modified="2022-05-25" version="1">MLSPETRSIIKATVPVLEQHGTTITSVFYKNMLNEHEELRNVFNRINQVQGAQPAALATTVLAAAKHIDDLSVLAPYVNMIGHKHRALQIKPEQYPIVGHYLLAAIKQVLGDAAKPEILEAWRQAYGEIADVFINVEADLYKEASWPGWKPFRVVSRENVASDIVEFTVAPEPESGIDLSRVPIVAGQYITVNVHPTTQGNKYDALRHYSICSISKDKGLRFAVKLEKSEEHADGLVSEFLDDHTSVGDRILLSAPAGDFALDKSLIHQNKIPLVLLCSGVGATPVMSMLEKQVAENPQRPVYWMQSSYDESRQAFKDQVDEFLDKCDNSHKFIVHTSSEPRIALEWLEREVPANADIYICGSVSFMRSMIEYLGRLNQSRIQYELFGPKMITIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6G2</accession>
    <name>A0A8J2X6G2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_12244g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG3 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6G2"/>
    <dbReference type="OrthoDB" id="296793at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048685">
        <property type="entry name" value="COG3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048320">
        <property type="entry name" value="COG3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007265">
        <property type="entry name" value="COG_su3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13302">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13302:SF8">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20671">
        <property type="entry name" value="COG3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04136">
        <property type="entry name" value="COG3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 3 N-terminal" evidence="9">
        <location>
            <begin position="73"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 3 C-terminal" evidence="10">
        <location>
            <begin position="239"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF88523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="752" mass="87155" checksum="B458F63D2DBBEDC5" modified="2022-05-25" version="1">MTRNRADSLVTSIAAHSNGIDSQFLPGILDDAYLVEKLQELSTRLGDREAHQAPILESAAIIPDPYAKYTLFSERLGSHIHEYENIISQSQKVNNELEEAIKMFHDISTNTKTFVNKTKGMYDEYTRLSLLHEQIPRILEYFDALDPIMRRLNHASTSNVVRKNSFKNMMGNIDASLCFLEEHEDLKDAASYRIRFKRCLIKACELISSYLNHILKQLYVEINEKLMSSIGSSSASKEALLYNKFASLAEEYRGQVIEIISRVNKDRYQRYRDELGSILNDCYETYFQTRMRLLHPSIYQRLNEITLQDKKLLLINFIQDGKTYFQQLCNDEYQLFIKFFPESESKWKINLWFVQLCEPVYDCVRVRVLRESDISTLCDALTLFGQYYEFEEESEEYQKQFSQIRYDKVFEPIVQGLQTTLILRVQVYVHQNIVKYTPTKDSFIIANRKSSLNDLRSSKEKEDPMVISFMKSFQDQLGADNVESLESYYPPLVRGLALLSRIYEMVNSVVFDDLAHHIVHDCMLSLKTAYNKVQHSTNDLNNFEVKLAYLKNLLMLRQQLQNFNIQYSVNETYLDFSGMETFFKSVTAGARSLRFRDSSVLSLAKGLVPKVVNNLVDARSELMLDLRNIIKDFTEAAAKSVIQNTLIIDNKGDDSTLIQKNMLLRQNVEDKFPRIYAEICIYVKDKEVVSHLIDAVQEIIIESYRSFYEEVSEKAQSGQLDKVQSSEVMYDDVFAEFLSSVTGKLSSSSKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6L2</accession>
    <name>A0A8J2X6L2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ribonuclease III</fullName>
            <ecNumber evidence="2">3.1.26.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_14026g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.26.3" evidence="2"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6L2"/>
    <dbReference type="OrthoDB" id="2392202at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034963">
        <property type="term" value="P:box C/D sno(s)RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030847">
        <property type="term" value="P:termination of RNA polymerase II transcription, exosome-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19876">
        <property type="entry name" value="DSRM_RNT1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000001">
        <property type="entry name" value="Ribonuclease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040540">
        <property type="entry name" value="RNase_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044449">
        <property type="entry name" value="Rnt1/Pac1_DSRM_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207:SF0">
        <property type="entry name" value="RIBONUCLEASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="RIBONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18497">
        <property type="entry name" value="RNase_3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="8">
        <location>
            <begin position="214"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="145"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="437"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="156"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="462" mass="52523" checksum="08655E362F31256E" modified="2022-05-25" version="1">MSSDSKKKNGVLKEQGSFDGPKDSLKGLAESDKDQCTVYQYSQMIQLEHAVTKLVESYERIITLSPNLKIYNQLLSNPEQVPVQILPALSRYKLKLAAELKSIDELDKVPLLKEIRGYESKFDSNPSSTKPILSELSSKEVKKLSSEMTHLNGMTEIEEEEEDDDDDEESYDPSKYSKDAKSSKWPPKLPEIKDPAVRARVFIHKSTIKDKLYLTESEMIKAHNERLEFLGDSVLNTVMTMIIYNKFPRYTEGQLSKLRMSLVSNERIKEWSFMYGLDKKIRTNFDITKDNANFQQGKRKLYADVFEAYIGGLIEDDPKHNMPKVRRWLNKLAKPVIDEVTVKDVGLESTENVDLNAKRQLYSLIGYAALHLQYVTVKRPTNMDPNTIVECRVGDGTVLGVGEGRNIKIAGLKAAQNVLANKPALEKYARLRAAVPRNESVVKQDENKPKKKKQKLEDGTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6N1</accession>
    <name>A0A8J2X6N1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_19394g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_19394g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF88002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6N1"/>
    <dbReference type="OrthoDB" id="4069481at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015891">
        <property type="term" value="P:siderophore transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035323">
        <property type="entry name" value="Fit2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17357">
        <property type="entry name" value="FIT1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035257768" evidence="1">
        <location>
            <begin position="21"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF88002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="150" mass="15037" checksum="3F0AD1C96FA34C81" modified="2022-05-25" version="1">MKFTTVLGGAAVLNSVFVYAAESEKTTKVITATNGDNVYTKTVTDDADPVLSHSTTRTVTVSNSAATYTKVITEGPNVESSTTKTLTLTNSVGSSTDLYTKTVAEAIESSGSSSASSSASSSSSSTSAGAAAAYGIGTGALFMGLVAHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6U1</accession>
    <name>A0A8J2X6U1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cation-transporting ATPase</fullName>
            <ecNumber evidence="13">7.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BN860_00474g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.-" evidence="13"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07542">
        <property type="entry name" value="P-type_ATPase_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000032">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000064">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000125">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000068">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047819">
        <property type="entry name" value="P5A-ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047821">
        <property type="entry name" value="P5B-type_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF8">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12409">
        <property type="entry name" value="P5-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="489"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="514"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="696"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="729"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1261"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1301"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1347"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1377"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1417"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="domain" description="P5B-type ATPase N-terminal" evidence="16">
        <location>
            <begin position="308"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="15">
        <location>
            <begin position="552"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="149"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="203"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF12409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CDF88724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1463" mass="165031" checksum="2ADE632651424E32" modified="2022-05-25" version="1">MNGRRFSNDHREHTHGNSLNRLSFSSAHSGSTAATLTSASNAEQNHTESYAGATFETVPSSIVSFHHPHSFQSGSVLGGSLASSTLRRGRRSTEEEALLDSAANYSRSPSRNPHFRLFTEDQIAAAEGAASTLEATDYDMNWDSTPAYEQQRLEESRLSSTRNSLHSNSVRSSNFSGISYDSIGERRRGRSLSRSRQLHPEQSVHSSVRSSSNSSRYSTRDRIPSELEDNGDEMLNDRSTNGSLPMSAREGFSENDGDNASGIADSHANGHHKKAIYHAEFLQPCHHEKFYPNLPAKLYYQRFYVAEEDLVIGIGGYRTSKFRLYIYYMLCLCTLGMVYLLLRWIPYYKVKLYGVKVPLGKAEWVVLESELGDFNIVAVKREWYNRPMSTVLPITKELADDNAADSSSSHHANGSNPNIPILISFDYRYITFIYSPLEDIFRSNNNWTDVNWVDLPSVYRGLTSGTCEDRTLAFGRNNINLKVKPTSQVLFDEALHPFYVFQLFSIVLWSLDQYYYYAACILLISFLSIIDTLIETKRTSQRLAEMSHFNCEVRVLREEFWTHISSAELVPGDVYEVSDPALTVFPCDSILLSGDCIVNESMLTGESVPVSKVPANEDTMYQLLDDFKDTQISNYLSKSFLFNGTKIIRTRIPKAQSAALAMVVRTGFSTTKGSLIRSMVFPKPTNFKFYEDSFKYIGFMTMIALFGFSISCIRFIQIGLDKKTMILRALDIITVVVPPALPATLTIGTNFALGRLKNRNIFCISPTKVNAGGKLDVMCFDKTGTLTEDGLDILGVHVSEPSSHNSFAFGELQHSVRQIFPKFSLNDCSSPMDFRARNFFIALLTCHSLRLVDNELIGDPLDFKMFQFTGWSYEEDFQEHAFHSLYDKRHEGDIFPENSDIIPAVVHPNGNEPHNRFTDNDPHNFLGIVRSFEFLSKLRRMSVIVKPSSDNVFWAFTKGAPEVISEICNKSTLPKNYDELLHHYTRTGYRVIACAGKTLPKRTWLYSQKVTREEVETNLEFLGFIIFENKVKDATSKTLQILREANIRTIMCTGDNVLTAISVGRESHLIESPKVYVSSINEEADVEDPFPVWRSVDCYEETLDLDEPQFSTSTGDYTLAVTGDIFRSLFDNDEKISESYRDRVLLKASIYARMSPDEKHELMERLQKLDYVVGFCGDGANDCGALKAADVGISLSEAEASVAAPFTSQVFDISCVLDVIKEGRASLVTSLACFQYMSLYSAIQFISITILYCRGSNLGDFQFLFIDLLLIIPIAVFMSWSKPYHKIARKRPSANLVSPKILVPLCFSILICLSFQAIPWLLVQRTPWYIKPIVGGDDAVQSSDNTVLFFVSNFQYILCAVVLSVGPPYREPMSKNVGFIVDVILSLFFSTILMFINPDSRLGSLFQLTGITTGFKVFILVWALLNYLCQLYVPTYFKSLFKKNHSSKKYKSILKKQNMSLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6Y7</accession>
    <name>A0A8J2X6Y7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin-activating enzyme E1 1</fullName>
            <ecNumber evidence="4">6.2.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_16182g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.45" evidence="4"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6Y7"/>
    <dbReference type="OrthoDB" id="10252231at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004839">
        <property type="term" value="F:ubiquitin activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01491">
        <property type="entry name" value="Ube1_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01490">
        <property type="entry name" value="Ube1_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2660:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12550:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000015">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.180:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.60:FF:000002">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.180">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, FCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.80">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12550">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2660">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, SCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.60">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, UFD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032420">
        <property type="entry name" value="E1_4HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032418">
        <property type="entry name" value="E1_FCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042302">
        <property type="entry name" value="E1_FCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018965">
        <property type="entry name" value="Ub-activating_enz_E1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042449">
        <property type="entry name" value="Ub-E1_IAD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038252">
        <property type="entry name" value="UBA_E1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019572">
        <property type="entry name" value="UBA_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042063">
        <property type="entry name" value="Ubi_acti_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018075">
        <property type="entry name" value="UBQ-activ_enz_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018074">
        <property type="entry name" value="UBQ-activ_enz_E1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000011">
        <property type="entry name" value="UBQ/SUMO-activ_enz_E1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01408">
        <property type="entry name" value="Ube1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF4">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16191">
        <property type="entry name" value="E1_4HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16190">
        <property type="entry name" value="E1_FCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09358">
        <property type="entry name" value="E1_UFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01849">
        <property type="entry name" value="UBIQUITINACT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00985">
        <property type="entry name" value="UBA_e1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00536">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-activating enzyme E1 C-terminal" evidence="13">
        <location>
            <begin position="885"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="764"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10">
        <location>
            <position position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF88691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1015" mass="112750" checksum="A5BE5A07D8488DED" modified="2022-05-25" version="1">MSAGEIDESLYSRQLYVLGKEAMLKMQHSNVLIIGLRGLGVEIAKNVALAGVKSLSLYDPEPVVLQDLSTQFFLGEQDVGKPRATVTQAKLAELNSYVPIKVLDSLVDERALQEFQVVVATETVSLQQKINLNNLCHAANVGFISTETRGLFGNAFVDLGENFTVLDPTGEEPRSGIVSDIEPDGTVTMLDENRHGLEDGNYVKFSEVEGLDKLNDGTLFKVEVLGPFAFRIGPVSQYGKYVKGGIFTEVKQPLKMSFKTLQQSLADPEFLYSDFAKMESTPQLHLGFQALQQFIANHGGQLPRPMNKQDAAEVIKYAQQLAASEPSVLGGGDAVIDENLVAELSYQARGDLPGVVAFFGGLVAQEVLKACSGKFTPLKQYFYYDSIEALPEQKQYTRTEETTKPINSRYDNQIAVFGLEFQRRIANLSVFLVGSGAIGCEMLKNWALLGLGSGSDGGIVVTDNDSIEKSNLNRQFLFRPKDVGKHKSEVAAQAVAAMNPDLIGKIDAKIDKVGPETENIFDDTFWQSLDLVTNALDNVDARTYVDRRCVFYRKPLLESGTLGTKGNTQVVVPMLTESYSSSRDPPEKSIPLCTLRSFPNKIDHTIAWAKSLFQGYFADSPENVNMYLTQPNFVEQTLKQSGDVKSVLETISDSLNHKPLNFDDCIVWARLEFEKKFNHDIKQLLYNFPKDAKTSNGAPFWSGPKRAPQPLQFDITNSDHLHFIVAAANLRAFNYGLKGDVGPPHIDHYKAVLSNVTVPEFSPRSDVKIQVNDDDPDPNSDRGAAGDDLSVLGASLPDPSVLAGFRLEPVEFEKDDDTNHHIEFITACSNCRAQNYFIEPVDRQKTKFIAGRIIPAIATTTGLVTGLVILELYKIADGKTNIEKYKNGFANLALPFFGFSEPIKSPKGEYNGKTYDKIWDRFDIYEDIKLSDLIKHFQEKEKLDITMLSYGVSLLYASFFPPKKLKERLNLRITELVKFITKTEVPPHVHTMILEICVDDVDGEDVEVPYITIHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X714</accession>
    <name>A0A8J2X714_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BN860_05424g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_05424g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X714"/>
    <dbReference type="OrthoDB" id="332390at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018501">
        <property type="entry name" value="DDT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028941">
        <property type="entry name" value="WHIM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013136">
        <property type="entry name" value="WSTF_Acf1_Cbp146"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32075">
        <property type="entry name" value="ISWI CHROMATIN-REMODELING COMPLEX SUBUNIT YPL216W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32075:SF6">
        <property type="entry name" value="ISWI CHROMATIN-REMODELING COMPLEX SUBUNIT YPL216W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02791">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10537">
        <property type="entry name" value="WAC_Acf1_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15613">
        <property type="entry name" value="WSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00571">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50827">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51136">
        <property type="entry name" value="WAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WAC" evidence="6">
        <location>
            <begin position="23"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="DDT" evidence="5">
        <location>
            <begin position="384"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="133"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="269"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="532"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="269"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="533"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF88239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1124" mass="131077" checksum="83D8888EBDBC1466" modified="2022-05-25" version="1">MVLYKRKPIVLPDPRPLPTDLSIYVWHIDETGEWFITYEEYLERLDFYTRHHFTCEITGTSCLTFFEALDSEETEFRYVEDRFPLKLREPVARFLHFNGVKRLDALVEKVYSKFKNDFFPGEVVYLRKNNKDISATSSHQPSPQPEDSNSGAKENGEVTHPQYQRPYVIKEKAQFNATTDPDTGEVIMPAHSKYMLIEEPHGSKSLIADQSQLHRDRTTFTKHLIKCFCKITLKRASSKMGAPWAVKEEYLPMYGLTMDWPPEMLKYKDDEPVTKQENSKGKRKKVDEDLDHQQDDENRKIDDEGKVKNEEETHFVSNKRKKTTEDDKEDSVAQEPAPSGTITSIVEDSVLPYQGSPHIFENLFHYTNFLESVPVNSRLPFKPFRETQKLLQVFQFLTTFGPKLYLSYFNLDQFITSLKCTDPHELKGEVVYVEFVKDGKESSFTEPESDWQRNPGIRQYIRQRNTERIRYHIVKDDPASEDIVDNVNHNGSGLLMECISALLRLFVNENGDWVSLVVEEWLDSKDEEYLEEDNVKSEKREDDKEDSNENSVFDEINETLEKCLNYRNINWAERLSKRQFNNHYWMLIILGIFQDCMHISLYTDIIYELVEKLVPSDISATQLPKQLWRNFCFKLTLDQKLNCIWILVDLISNFSPDVKAAVEESMDLCGQIRSERFRFAKDLKAETSYLNMMQVDLNVLEQAGNRDEAAIESHKERISPQAAKVDQLQKDKQFLDKKLMENDLQRLKTLGLDRYGNRYYWMDLSGIPINVGDDTEELNYHSGRLWIQGPAAAAAQYFLAISSEELNEWKKLTEEMGKVYATRHVFHICRTTNGAYKYVEGENETELCNSDGIINCLIELTPIQKKIIDETPDCLLLSEEQWYSVDRVEDVRRLLDWFDNWGRREHDLLKQFKLIEDTLENVYAVRDKVLHLFTFDDEEEQLLKGLQEYELNDVELNFGAERQEEATNGEKIREKDDEKEEEELEDIAGKIMELDDSSKTRKILNMIRELEVRRDELLAKRQATISAESSGGRALARAEKKRLKNVIDGKLEKQAEILTDLLNHRHFNAMEDAINWKNHLATKILGTPLRKNATDSKKSKNVDTVDAKLKEIMRHTSRISAASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X730</accession>
    <name>A0A8J2X730_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ZYBA0S03-03994g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_03994g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1562405at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019441">
        <property type="entry name" value="FMP27/BLTP2/Hobbit_GFWDK_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019415">
        <property type="entry name" value="FMP27_SW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019449">
        <property type="entry name" value="FMP27_WPPW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045167">
        <property type="entry name" value="Hobbit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678">
        <property type="entry name" value="ANTIGEN MLAA-22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678:SF6">
        <property type="entry name" value="BRIDGE-LIKE LIPID TRANSFER PROTEIN FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10344">
        <property type="entry name" value="Hobbit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01214">
        <property type="entry name" value="Fmp27_GFWDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01215">
        <property type="entry name" value="Fmp27_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01216">
        <property type="entry name" value="Fmp27_WPPW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27 SW motif-containing RBG unit" evidence="4">
        <location>
            <begin position="1011"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27/BLTP2/Hobbit GFWDK motif-containing RBG unit" evidence="3">
        <location>
            <begin position="1123"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27 WPPW motif-containing RBG unit" evidence="5">
        <location>
            <begin position="1484"/>
            <end position="1989"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1726"/>
            <end position="1749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2295"/>
            <end position="2359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2484"/>
            <end position="2512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2295"/>
            <end position="2307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2326"/>
            <end position="2339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2347"/>
            <end position="2357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF88878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2532" mass="288169" checksum="8337EE6A7D63CF3D" modified="2022-05-25" version="1">MTVMWSNTLSDLLKLLVVFFLSAICIQTLVSLLISQILYYSNSPIKGIFFRIWGPSISAFKLTTICFKLKINKIHISWARRFIITLEDVDVELLKRQAKGEETKESSTGGSSTNGGFSFENEKIQFTVGKRLLAIIRWVLPLRLYIRNVIIVLPSGVGVKVDLVSSAITEFSEKRINAEVFVHAAKCLESGDTVHHIGYQFKAKVRDEIGANGGKTVVTIRNWLSYLRVAGVCISLPDLLARDLNRSGSEPIKAEKSPVQLETMLREKLDKFKAPLKAMKVLDFKMENIFFIYEGIAQVTISNAQMLLESVHFYSYGTNLEFLPSKSPVLNDHQLSVTANSIVIEFKEISSVRIPLFNAIITTDCVSRLFMGVPLSDAKILTNVNIINPTMVVTMAHVLKIASLIHNYKASKAERLATRQVDAEDDIGDEEYHDVASSEEENIKMLREAAIHELKQKFSVYPNAIIEFTISNLSATLQLSEKENILFKLYNAHCLGFHQNDKKVFVTGSSFNDVSSRPELQYFEGDPFTQQLYNYLQVIGAKLTFLKLPSDEREPALSIPICGFDRSDTFMDDISDFDFRVRSTLRHFSFTLDNLDILHKITEAMVKLFAPNGGNEKSGYQNSYSPRKVKGRSFVFAWSVKLRLKDFSFSLLLAGFLPRYLDPVEYEGINLTDLGRGTILLMNEALLKIDPEEKDFKISSLSLIRIMEHGHGRKITDVVFGFEKLVMSWKSDHNGRIHLPCIRFKLDPNILWSVFFIKKVFDSYLLPFKKAKHSLQKKHPENQQKKFDVRIGKIIVDLTLPQDTVLLLSFRDIILAAEDHSIRVASITAFVKSVYVKVVDVHVPLLIIREFEFKVMESAGKKTFDLSTSLIQFDTEYHFKFYTLLDNAISFVKCIKQMKLAFSNLDKFHRLYPSQEPPRELPTIRLFAKRFGIKVKEDPFEQELGLIFKVGVLEQRERLEKLREFEILKDTFVPPVEVPPSSAEVQPLEQVVENENVYCENEINENQWIKNARKRLLENFSTSWIARYREARMTFLGLPSRIVKHQELGRTYFFFTVNEGTTVAELAINNLDFNVGPPSFSLPQYADFIYKYGKKVPKDTAYTLLIIAHLSIRTDLWELRARDYPLPAISFPNTFTNGDVVFGEKMPDECSLRSVYVPFVPSANTDVYSATNTIYGTHIIRTLNSIKSYFNITTSVHSAVPATLTWGKSLQPGYESLMLWFDVLTRPKEDPSPKLGFWDKCRFLFHGKWIYDFSDISGIQLNIKGSENPYKIADDGAGLTFFWSGGTFLRIHGTSDPTEFLKIESKKFQLAVRDFTEHNKFEKVFSSLEGEVIWTLGLLFEEGNIHDPGDFPRSPPSRPHFDVISKNPKAVSNPEEYDAFRGFRTAFIHMSLGVYSSKKNSSNSIYLAPHGVAHFLKWWSLFHTYTSGPIRQGPLFSSAQPNKAKFGRSLFTVKYQLCLEPLVLTHVYRHYSDFRGNGSVNFTGLKCRVNSLEIDLHQKRIKLLHTNEKLKKSKAIWSFKMSTGQIECAEADVRILSTVFDQSDVEKMAATKLGKHVKSEYSDRSRSQSPVREGLQEHSEWYDYEDYIDLDQVSLETSPPLRLEAVPLMYFPRLSYFRQINDSGYIVKYPFGDEESHHCIMGKDHSERTQEHLARSRGKEIEEDIQRLTDELEVMKKQHGKSQTYHTQYENLTQKLHEAKHRLFVIESILTDLKLSADVPNSFHGDEEGEASIMSSNGKSTGGKVSEADDGPYDDTALLRIGTIETFRTMRCESNVNLASSYDNRFFVHNVQFKVNKKVRHHLIDYFTSAFERKSMQFFLTYKSVTILNELLNAVMASTPKPSTSQEDAPDSGPELSNAEFMERFDDLVREVPSGIFDYIDSYLIRLISPQLQLMSESEPNVAALITARDIEVGIINIIQFMDDAGERVPMDLNTLVEARLCVVSKDIQVFTLMKNDILAKGGEGFHRNGYGVEDKAKLWPPWIPMEVCFEGSLLEEDVFVRRRSMYFMHTIPNPLFFSDKEIPGLSGNPSSRLGFPGLLITSTSQQYCAVYNIVEDLLDFGDSLDDKVAKLANVMLAEEVKYNLKKLDTSIVTNLQRKVKEQYYTREFLKLHSVNTFKKTAPVLTLQIQTTMLELFIIMTAIKKNYDDLVRGHKTGKRRHEWRVSSDELIWEMFDDSRKPFITIALGSSIYVRHEAIDGTNSNLVALSSVKCFNQQENPVYVEIVAPFDKDSHYSKNVPMVQISWNLGPPVGGIGDLQKMVVTLQPFKFKIDYITSDKIMNYLFPKSDRQAPAAVTSSTALSPSSSRQPAMATVINPQGGRRSRSSTLELSASFSNSSEDADGEDLSSVQSGSDLSSMRKKASRKIATHLFSQPDKNISEMVKRSSTYFNVGYVHINKLIMSVCYKGAHHLLTDVNNLTVRVPHLEYKHKLWSREEFIAALRRDITRVVLGHLGNIIGNKFIPHKKENKHKVSMDISQLLHLDGKGSGSPRHPNRQISHSPSVPSWEGDVESLGVTTDEEIGLGPFNPAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X777</accession>
    <name>A0A8J2X777_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BN860_09450g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_09450g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VPS53 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X777"/>
    <dbReference type="OrthoDB" id="10261632at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.110">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 53, C-terminus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039766">
        <property type="entry name" value="Vps53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031745">
        <property type="entry name" value="Vps53_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038260">
        <property type="entry name" value="Vps53_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007234">
        <property type="entry name" value="Vps53_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12820:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 53 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12820">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16854">
        <property type="entry name" value="VPS53_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04100">
        <property type="entry name" value="Vps53_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vps53 N-terminal" evidence="8">
        <location>
            <begin position="7"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Vps53 C-terminal" evidence="9">
        <location>
            <begin position="613"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="776"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="794"/>
            <end position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF88404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="824" mass="94542" checksum="B5D753E8D90F6341" modified="2022-05-25" version="1">MISDSIDYDPFEDITSTLSAKDSLGEIDQLICATRQYKLQLTAEIEQTEKAKLDATENVTETKFDFDKVFREIDETKHFSSTTQSTISQLTEGISHLDNAKRNLTQCMTLFQNLKILTDSYFQCKELIRRNAYKEMASSYKIMCSLAENTFKPHKSVDDINKLLASISRLKAETFDKIKHSYSRVLSGKAAESEALEAELREGACDILDSDSSDRAQIIDICLKKLLSEIGEIFRVDDEAGSLENLSRRYIFFKKILNSFNSKYARYFLPEWQMSLKLTSAFYKMTAKDLEILLKKEFQDKNASIDLFMGALQATLDFEKYIDVRFSNKMQETKLSVYFEPYLSLWVSHQDKMMNDKLLSYMGSGDLSTTLGDSLVVPSSADLFRTYRTVLTQTFELIENNTNNGILVALANFFTKWLIAYSNRILKPLVLPDNEEIQNKEEAIKYTVTMINTVDYCSVTVGQLEDKLAELSPDPEKISQNFAAVKDSYDGLLAKGNSILLHRILALDLAFVAREFNNIDWARVVVENYSRYMVTLKEILCFNTNSDQKSTLQLILSQFNRDVYSWNFLDKVIDLITQDFTGYIIRLLQPMPPFATLSNTRKFDSAKVTNIGEQLLLDTELLKEIFHSLPGSVSDEVNSTQTTAYKRVQKHIDTNLTQILQFIKVLMTPLSSVDDYHEAYKKLTNDNTSSSVWAYLLTLKGIPWDLTLWKQHWSAFDMDRDLENGSDGNNDLFIFKWNTKLITQFEFNMLRIQEPLWAKFIKDDLQIKPPSRTISKIRVPSKPYQSPPLKGPEQPQSPQNSLGSPLANVRNMVTNNKFFDRSGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X7G8</accession>
    <name>A0A8J2X7G8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S03-10594g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_10594g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X7G8"/>
    <dbReference type="OrthoDB" id="10251727at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF89161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="407" mass="44873" checksum="35869FC76AA469FB" modified="2022-05-25" version="1">MGKKSRSGAQKSQSIGTPTKASPNGKLSAKNSPKASPKSTPKGSPKSTPKSTPKSTPKGSPKRSPKISKNAKLSKEQTFRSRVVHTLDQLRNFEDKQGEDDKTLLGGDEELGRYIQLIAVNNSSFTGSNKKFKPRLLDIKHSLYQAWQKASVTMVKDFKILLVLKDADAGKVEPEQIVGESEGAPSPTIVTAKDLKTKYKAFEARRAFISDFSLILADDSVITALPKLLGNKAYSKVETTPIPIRTHANKEFSLAALQNHFRKIYDSKLPVILPRGTTLNIHLGHLDWFNTEQLADNVESIVNQLKDSYKIRSIFVKCNDSPAVPLYYNQDVLDEISKVKEEKPAVEKNNEVEIDGVKVNLSAFDSALAEVADPQELKSIFHNKVKSAKRSKPEEPNSAPKAKKAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X7N3</accession>
    <name>A0A8J2X7N3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S03-12838g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_12838g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X7N3"/>
    <dbReference type="OrthoDB" id="1888931at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050085">
        <property type="term" value="F:mannitol 2-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000090">
        <property type="entry name" value="NADP-dependent mannitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF13">
        <property type="entry name" value="L-XYLULOSE REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF89254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="292" mass="31681" checksum="6C31FB663A91E3B6" modified="2022-05-25" version="1">MTFQSFIRGPSEDKNVPQEPPREQYANAKGFLDLFSQKGKLVVVTGAAGAIGGAICRGFASAGADLCLLDYKYDPKFATDLSNEYGIVAKSYQLDITKADQVKNCIAQIQADFPERLINTFIANAGIAWTNGSILHESATPEAWKRVMDVNVQGTYHCSKYIAEVFKEQGQGSLILTASMSSHVSNVPNYQTCYNASKAAVRHMSRGFAVEFAHLTTPAGRIRCNSVSPGYTDTILSSFVPEEQRAQWWGLTPAGREALPEELVGAYLYLASDAATFTNGTDIVVDGGYTCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X826</accession>
    <name>A0A8J2X826_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mediator of RNA polymerase II transcription subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mediator complex subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_09230g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the Mediator complex subunit 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X826"/>
    <dbReference type="OrthoDB" id="5310959at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019680">
        <property type="entry name" value="Mediator_Med1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10744">
        <property type="entry name" value="Med1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med1" evidence="8">
        <location>
            <begin position="11"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF89645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="496" mass="56372" checksum="B05B4057EDB2D076" modified="2022-05-25" version="1">MASDSYAVQLDEMIQLFQEYKSGSVTLDNITKLCQTLGLESFIDDIDSETSRLSTASKIIVIDIDFSKSKGRVKDVKLVLASNFDNFNYYNDPSGQASERGNILLNSLTEYPDLHEFHHNLKFLHLLDAYSSINIDASNTVGNGNFGSNNGSGNNDSNTASNDYGGELDLFKYYTELAQFLRQYFADNSAPFRVETNLNNRFGIYILTQEGQQLLVKIMFQKSKDQQHRLYEYVYSEGHKEWINESPESYTSGISLVMEVNDENCQIWFPRNYVPQELILDEGEARKKMNDLNSLDVINSIATNGSRGKFMVMNDFTTQLAAFKKFDISNDNLELILEILRWMQWSRTVLNEIWAALGASKGQTIDAGMDMSEGAAVAATKGRRSSVATDYRKRRHSSKNKRPSITEATMLKEEGLQQFNLQELMKDQDIDPKENSALLEVDKMQVDDQAIEDTDAQLQLVFSEDHVSLENIAHCTLYDGIEKWTAFMEAFNKCRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X835</accession>
    <name>A0A8J2X835_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ZYBA0S03-10286g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_10286g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X835"/>
    <dbReference type="OrthoDB" id="6509908at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032218">
        <property type="term" value="P:riboflavin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF295">
        <property type="entry name" value="TRANSPORTER MCH4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="257"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="404"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="469"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF89148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="504" mass="54673" checksum="6BBF4741BEF0B764" modified="2022-05-25" version="1">MLPKDAIVGFAKALVTKKKNGCPVENVEMQENLTLDYKSSGYAGNYLPGGSMVKKNNNEVKNYEVRNQVMNDVTKDTPIVGDNEFDEEGEFPDGGFQAWLAVLGAFLGLVPVFGIMNSIGAIESYISKHQLKDVSSSSVSWIFSLYLSISFLSCILSGGYFDRGGSTRPMMTGTVIYVGGFFAMANCQKMYQFVLAFSVLSGTGTGVLMTPLVSVVATWFHKRRGIACSMATMGGSVGGIAFPIMLRKLYSEVGFPWAIRIMAFISLGCLSFALKFAREREKPVVEPFHSTREMIQWYASSALNWRYFLDTKFLFAALAAALAESSLTASATYLTSYSLARGNSQSVSYSLITANNALGILGRYVPAYLADRCLGRFNVAIVTISLAALCNFVLWLPFGGHIRLLWAYVCLYGFATGSILSLNPVCIGQISSTQDFGKRYSTACFLEAIITLPVLPIGGAIIGKGSVSGYNKFIVFVSVLMAAGAGSYYISRSLCTGPRLNAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X899</accession>
    <name>A0A8J2X899_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_10330g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_10330g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X899"/>
    <dbReference type="OrthoDB" id="1874341at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019183">
        <property type="entry name" value="NAA25_NatB_aux_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09797">
        <property type="entry name" value="NatB_MDM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="789" mass="91666" checksum="9DFE0DC5043EE277" modified="2022-05-25" version="1">MGDKSGEEIFQLIQRANFKACNAKINELKKQSPNSSYVRVLEIYAKYKQSPSKFNYEKSLGTFYGLEGTEITGDTRALKLLHNMFLEMGKYDESLHVYEKAVLKYPSFEVSYQWFNRSLDDGNYKMMARACQQMGKWTDSSEGSLSSRDYTFWYALCTVALFKFQRERISKVEQKLLPQLALRSLKALKPFQSTEELIAYCHVCETLFEDKSKEIVDNIWPTLGKSVNLYCKNFLIRHVEDPEQMVEACKSILSKMDDFELICKLIHASRNLNKNRQDIICELEVLVGDSRNTRLARLELDLAFNNGNITIDSLLYYLAKYHNKPCCPPDLARYKQHLNWNEVRETMGKFESDLNHDANAFQMGFLAISSLEAYNKHKSTLSKKAKMDYSSCSVFIMDLVYDLVVRNEPTMQNVLLALSMLENYQTYDPYNYDTSTWIIILYMHLGCVPAAYSRYLDLKVKNLQNDTVDFILYSRFATLFPRKEHDYLKQLQTNKYRLYCVSGNRLPQFIQIALEKQAYSKIIGMLDFRSRLAKSSMKWINIIEELQLARLCNDKRHNLLRSMDENRKAMELLGNMQFSDNRDWAILGSKISKDSLPSVLQYMDISQDYISLRLIIECIIEYIPSAQADPKLDQYLQTVLNGRTLEETLESNLDQVEAWSFNVFYDLYKNNGAKLPSFLEQLSSQCNQDTTNWKLTHSYLTKLSTLKTLDSFKRIKDKQLKQHIKGHIRILRDKCDELYQTYIGQLVQTCDNLSKGSSGELLKKLGFVPLSSESLKGPLTIVQKTVRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8J4</accession>
    <name>A0A8J2X8J4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MICOS complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_15412g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8J4"/>
    <dbReference type="OrthoDB" id="2399148at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044284">
        <property type="term" value="C:mitochondrial crista junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019166">
        <property type="entry name" value="MIC26/MIC27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033181">
        <property type="entry name" value="Mic26_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28268">
        <property type="entry name" value="MICOS SUBUNIT MIC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28268:SF1">
        <property type="entry name" value="MICOS SUBUNIT MIC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09769">
        <property type="entry name" value="ApoO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="228" mass="25353" checksum="329CDF62E9082BD5" modified="2022-05-25" version="1">MAGELDFYREADVVKEAVIPKQDGEGLSSDAKEAHVESASQSSVGLVGDNRLIHGISVRYPEYLVKFFHKIRVNLNGTIERASNTLEEKSSKYYKHEQSITSTIASLHSDPREELVPGFTYIAVAAMTGSVLGKGRNFLYRFSAPMILGAACFSYVLPKTFQNTMHMLHDIESQYFPYAVSRQDALLREAKLAACRTASFAEGSVKLVQHVVTSTRHCVKEWTGLNVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8K1</accession>
    <name>A0A8J2X8K1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 12">Adenylosuccinate lyase</fullName>
            <shortName evidence="12">ASL</shortName>
            <ecNumber evidence="6 12">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_15764g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = fumarate + AMP</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="6 12"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8K1"/>
    <dbReference type="OrthoDB" id="406045at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03302">
        <property type="entry name" value="Adenylsuccinate_lyase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.60:FF:000001">
        <property type="entry name" value="Adenylosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000005">
        <property type="entry name" value="Adenylosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172:SF1">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 12" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Adenylosuccinate lyase C-terminal" evidence="13">
        <location>
            <begin position="374"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF87845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="482" mass="54113" checksum="7F1DA6768AAF04E7" modified="2022-05-25" version="1">MSDFDKYLTPLSSRYASKEMSSLFSMRNRFSTWRKLWLNLAIAEKELGLDVISDEAIAQMRDHLVITDEEIAQASAQEAIVRHDVMAHVHTFGQTCPAAAGIIHLGATSCFVTDNADLIFLRDAYDLLIPKLVNVIDRLAQFAIKYKDLPVLGWTHFQPAQLTTLGKRTTLWIQELLWDLRNFDRARNDLGLRGVKGTTGTQASFLALFHGNHEKVEELDQRVTELLGFDKVYPVTGQTYSRKIDIDVLAPLASFAATAHKMATDIRLLANLKEVEEPFEKSQIGSSAMAYKRNPMRCERVCSLARHMGSLFNDAVQTASVQWFERTLDDSAIRRISLPSAFLTADILLSTLLNISAGLVVYPKVIERRIKSELPFMATENIIMAMVEKGASRQEVHEKIRVLSHQAGAVVKEQGGDNDLIQRIQDDKFFSPIHAELDALLDPSTFVGRAPQQVEKFIGNDVKKALSPFEKDINDVDVKLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8Q4</accession>
    <name>A0A8J2X8Q4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_17964g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_17964g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8Q4"/>
    <dbReference type="OrthoDB" id="10261452at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045112">
        <property type="entry name" value="PPAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661">
        <property type="entry name" value="PETER PAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661:SF5">
        <property type="entry name" value="SUPPRESSOR OF SWI4 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="2">
        <location>
            <begin position="26"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="424" mass="49072" checksum="85B01BF998A75BCA" modified="2022-05-25" version="1">MAKRRTKKRTHVPENTEEFSKIPKSMVIRVGQTSMANHSLNQLVKDFRHIMEPYTALKLKERKSNKLKDFVVMCGPLSVSHLFIFTQSERTGNISLKVARTPHGPTITFQVDDFSLNKDIKKYLRRPKSLNKEDVLDPPLLVLNGFNSFKNRKDSEDDEANVEKVVVSMFQNVFPPLNPSRTQLNSIKRVFMINKDPETKEISLRHYFIDVNEVDISKNLKHLYKAKNNLHKAVPNLHRKEDISSLVFDHDINAYTSESEIEDEAVVRMIEKKDVKVGTTEPALEKRPQEQTASRKKAVKLTELGPRINMKLIKIEEGICSGRVLHHEYIQKTDAEIKDLIKRHEQKKKLKEKRKKEQEENVNRKNAVKEAKKKRKMERRQHRREQGARESDISSDGDVSSNNEGDAQDNVSETSDSEISSEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9C8</accession>
    <name>A0A8J2X9C8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ZYBA0S07-03686g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_03686g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DOP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="297643at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040314">
        <property type="entry name" value="DOP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056457">
        <property type="entry name" value="DOP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007249">
        <property type="entry name" value="DOP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056458">
        <property type="entry name" value="TPR_DOP1_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042">
        <property type="entry name" value="DOPEY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042:SF24">
        <property type="entry name" value="PROTEIN DOPEY-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24598">
        <property type="entry name" value="DOP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04118">
        <property type="entry name" value="Dopey_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24597">
        <property type="entry name" value="TPR_DOP1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DOP1 N-terminal" evidence="7">
        <location>
            <begin position="18"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like middle TPR" evidence="8">
        <location>
            <begin position="369"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like C-terminal" evidence="9">
        <location>
            <begin position="1223"/>
            <end position="1649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF90553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1668" mass="190892" checksum="2865A719FF2A9263" modified="2022-05-25" version="1">MSLPLKPLTIDSNNKQLDSKEKKFKNHVLRALENFDSVTEWADYIASLGRLLKALQSWTPQFKNVKYYVPSPYQVSRRLASSLSPNLPAGVHQKTLEVYTFIFEKIGQDALAAECNIWVPGILPLMSYASMSVKTHLIEVYDNYLIQLPSSTLKLLAYPMLASLFPGIDDESSEFQPVTMKLIDTFRENLDNDSLFWQCCFLIMITSKERRLGGLVWLVKKFPSLNAVPHLVMERNKRAEKIGAIDELNAKELKEDAFSTLLPTAKDLLSPEPGLLIRCLVSSLDSNNDILIKRGIWDLLLQRLHLESPVLNVLVSPSDKKLLIMACCRNILDKDMSLNRRIWNWLLGPTASATLGNGTDQGIEDNNEYFVKHGFQSLIEGLKDMIEGQQSITTAINVCISLMDRWQIGSLVVPEMFIPLLLAAKKFSQNQQVLKSSSIFFDTVETNTIWGKIFDYLMEAGDLDFLEFVVTSYDVTNEEEIVVRHLPLILLAILSFHGDNMENFRINRYSVCNQLLEIIPERAFLPLRFSQMSFTSKVDTSKILKNISKYYNVISDPTSSQDGEELVDFPPPFSAEDLTFYVIRRAHDTLLENLKSGECIAESTRIFVEICEKIPESASDQKNTSSDWSDDSLVEALFNSLDNSDKEMESVYGIADLYSNYLASRMCFLQSVRLLKLIISSLWDYLVEPHTQLRAVKGLRLLQKCPYTEYMEGALGNAFIKEKEISRCLIVLELLWNQLEPNSDVLRRPVELMIDELFDEQNPHYLSVSKWILSVINSGSSNRLFYLLTDDLLRFPFLKRDQLQDMDDLDMFTYRLQCLTSVLKTNNGVVLENFNSELTSIDSLADWENEDISTYKNLVIVVVMRFLHLKNNNNATSIRSALLLLEYLLNGSEQNFKWIVMFLLQMSSEYISKSGLESDLIAVSLLNIVSKVLRLSHENGIKLDVFDDNTTHLKYVDYLITSVSSMESPLIVTSYVKLLSESLAYFESSIFRMMLPLTASIVQCVYKLLEKEKKHGGYYQSIALLLGGLEELLAVSHKFLIADESEGYFSGVGSRGDFLQNMVSNVFSSDFSANDIKIQGERDIILQSFKEVVTCTFHIWSWAHEISANAVKSPNMNQTNHNSYKFKFTAKKLLEKLFLLEPLEVMEDLVIAPENDNKLTLIHVLDGNRPALTIPYFFIGIVFRQNKNANVRFSVSSSGGKVTGNHLESSLMNKLDSQGLISFLLKYASSLENAAVEEFYGDFAAFFREVSTNYNLYGPISTSILKFVGIIAEKLQKSRFGEQKRVRREVSDIFVKYLPNGLADSPFEYKNPKQSFENLEYVVRSLQYILNDSINGEKYCSSLAIIVSQCVAPFIKSKTESAISEYVLKLGLQISKVGEKVKSWRLLINDFFMDEKKFILAEDSLWKDIIYYWSHYPENKSKIINDLITAVDSKAAGMISFNSWSESESHAKAQNLLRISFLLMISPVDSYLLQFQSLVSLVCEYIVSKNNKLKSKCWILLRALFLRFSDSHFNEHWSMLTYCLQTNLQEFFEALHIQEEIDLDLLLQTCKTLDLLLALNFEGFSGTNEWLFVIDTINCIYKEYPFMSLVDEIYEYKEFGRSSVEDVEQLDKVQIRVPLLVGITSIKAYTQLKNFFQNLSYAHYEFIYSLQEVDRCRCEEDLFSDIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9E4</accession>
    <name>A0A8J2X9E4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S06-06502g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_06502g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10001928at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056737">
        <property type="entry name" value="Beta-prop_ATRN-MKLN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503">
        <property type="entry name" value="MCG48959-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503:SF2">
        <property type="entry name" value="NEGATIVE REGULATOR OF SPORULATION MDS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24981">
        <property type="entry name" value="Beta-prop_ATRN-LZTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Attractin/MKLN-like beta-propeller" evidence="4">
        <location>
            <begin position="132"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="631"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="841"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1156"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1442"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="634"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="696"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="714"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="727"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="779"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="841"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="859"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="880"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1168"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1203"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1220"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1231"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1290"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1444"/>
            <end position="1467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1469"/>
            <end position="1490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1568"/>
            <end position="1581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF90351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1581" mass="175247" checksum="9159A96F84F30C3C" modified="2022-05-25" version="1">MPLLQPSLCACYPLKLPPLPPSLADADEPVKRKWMLESRTGAAVELTRSDIFVHGGLTIPLNLTQINSLQIQKELMLYFAKEKQTGSLFRKLSDWISPEIFFLDLVSRTWKRIDTKVERQNGSVDYDDGQDGSHLHERFFHSMSFSNSCLYIFGGLMVSPQNGYELIASNELWRLDLKTKKWSLVSKNPQISRRFAHSMHTKNENIENRDTKLVIVGGLNNMDQPVHKVDIYNLSKGYWESEPEAEGYKRVESNIDGKSVSHLKESNFSILIENNEAKVLTLVLYGGHNDEFHDRQDVDSKPNIPPLVALPLVADSKGMLMASNDIRVRDSSEFPYSLQFPTGSYFSHTIITAGFHPNCQASSFQCFVYDVPSGKWTKMGTSCDDRDYHRHRFWRAFVWQSHHQTLLLGTKDDDFNLPSVQKFDWLLSFGLPLISISHRLSQSAGHRSTILKPMPSPHNKGIPDVLPGNPEENINESSGNDFRKQSFTSSATSQFESYIRYIAPPVQMTSISSVFPPYAMVLGKDALEIFGKPLSDFEFITEEGDSIGVPIFLLRKRWGRYFDMLLSQGYAKACLDYEANGEPSDMIKFSPYSSQGFGSYPKNPETSSSGSLENYFNSARQQQRKFGVNKFEDTSNTSGTSSISEEPQHDSSHLAAMNHSPPFYENDEEDPMSPQGAYSNFAEYKNASSKSIHRIGTSSTTSSSGGMVFRVPFQDSSTGSLTEGRSAKSHISRDKRRSSSVATSALDHLRPNNSIEQLRRASQPDTTLTNDDSRGAHPSARLSINSNRSSRNPSVASENSSISYVSSSSDRMGNSILPHNSNGSSSGSAVLGVLTVPLPPSTAAPNEPLPAAPYEGSARTGSITDFGLSNKSSPLSSRRPSYDRSYHAPDSRFHSDNFRTSLDSQVLAENTQNESSGENSSQHRTLFAKLNPRSGDGSATSFMKPIKSADGNRLSLGSNADSNTSITSAGFDWEPLLTPRTLYMPWPTATVRAFAEYFYIGQVNGKWSLAPVALNLLIISKIYEIPLLHNLINEVLYSIIGRKEDSLFVTCNSLMETMKKKLRNVLNGDENLVQVHAQRNDIYLELERLKQSLENIDNGFVDLSLLKGVSRSLSFSTAESEDFGQENYGSSECSANSITGPIPTVFAGGPRDSHNSIGSIGYPPGLSFQGSRKSSSVFSPRVKKKSSLSKEVNLESVESNSDDPEKPRNDEQKHRDQIPIPTNINNKNYGTSSLNTSSSSSFTSTCSSMSDGDDQTNVGGSGNNRRDVPEHFNSTIIDARTNKEEEESDHDYNRNLNTDKKEENVENDSNSSLSDVDDLNSDMGLLSLNKMRRKLAGRLDLDESVDPLLKIDPTSQANNCFTKSHSHKHSKNCYSPRDSNLTLESLASPNALPPVDYVIKAIYRTAALVNYPRLMVRCLDCIEISRRLRKIKKRIASEFVSMEQELRRSATEPHAAAGREQRLERPNLEWSSAKGSFQEGSPSSTMSRSGNPKLDSRGGTAQPPQLSPRTSWGKDDDSIKSSASQSKKFLKSKIFGESPTPPASTSAFMNPAFMPPPPSSSKNKKSHSGSTSGFSFFGMRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9J9</accession>
    <name>A0A8J2X9J9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ZYBA0S07-02344g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_02344g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18020">
        <property type="entry name" value="DEXHc_ASCC3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18022">
        <property type="entry name" value="DEXHc_ASCC3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000198">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000002">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000231">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000241">
        <property type="entry name" value="Antiviral helicase SLH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000102">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000024">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000004">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000001">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000062">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF13">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039073">
        <property type="entry name" value="BRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="297"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="521"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="1149"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="1355"/>
            <end position="1566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF90494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1962" mass="223305" checksum="B84C5E4A7E43A6B3" modified="2022-05-25" version="1">MAASYSIHSAGSYMQAMQSMVDSTLSLTFDRANTIVPKIDNESKNGNATGSSSGKDYIEKLSQDENDWDDIFEQLDDISFDKLKSAVESYGKINSAATYKKLSRLIEDSSSPLPDAVLLDSILELVYSQDKNHLGKELLDLLGTDNMELIDFLLQSRDTIISQPKEHVLQMTEQAKDLPKFLSQQDIRDQYSDNAYKAKNQKLDPAQKIVKYPHVYRKYEAGNSSMMSLSGQKFSLPEGTTRIPYQTYEEIIIPAADPDASKRTFYVKLLKVKDLDYFCREVFTYQTLNQIQSLVYPVAYNTNENMLICAPTGAGKTDVALLTILNTVKQFTTVGNEGEVNIDYDDFKIVYVAPLKALAAETVDKFQKKLSGFGIQVRELTGDMQLTRAEVIETQVIVTTPEKWDVVTRKGNGDDNLVSKVKLLIIDEVHLLHEDRGSVIETLVARTLRQVESSQSMIRILGLSATLPNFMDVADFLGVNRQVGMFYFDQSFRPKPLEQQLLGCRGKSGSKQCKENIDNVSYEKLLSMVEKGYQVMIFVHSRKETVRSARTFIGMAHENHEIELFAPDPTMKNKYTKELSKNRDKDIKEIFQFGFGVHHAGLARTDRNLTEKMFKDGAIKVLCCTATLAWGVNLPADCVIIKGTQVYDSKKGGFTNLGISDVIQIFGRAGRPGFGSANGTGILCTSSDSLDEYVSLLTQQHPIESKFGSKLVDNLNAEISLGSVTNVDEAIQWLGYTYMFVRMRKNPFTYGIEWEELASDPQLYDRRRNLIVNAARRLHSLQMIVFDELSMHFTAKDLGRISSDFYLLNDSVEIFNQMCSPKATEADVLSMISYSSEFDGIKFREEESTELARLTDTAVECQVGGSVDTSQGKTNVLLQAFISQSRILDSALASDANYVAQNSTRICRALFLIGINRRWGHFSKIMLDICKSIEKRLWYFDHPLCQFDLPENVVRQLRSKNPTMEHLLELDADELGELIHNQKLGNKMFKLLSRFPKVIIDAEIFPITQNVMRVHVELVPDFVWDFRIHGNAEFFWVLVEESDKSEILHFEKFILNKKYLSSKHEMDFMIPLSDPLPPQVVVRVVSDTWIGCEYLHTLSFQHLIRPHNETLQTRLQRLRPLPITALHNPLIESIYPFKYFNPMQTMLFYTLYNTNENVFVGSPTGSGKTVIAELAMWHAFRDFPGKKIVYIAPMKALVRERVDDWRKKITPVTGDRVVELTGDSIPDPRDVRDSTIVITTPEKFDGISRNWQTRRFVQDVSLVIMDEIHLLASDRGPILEMIVSRMNHISNQTNHPVRLLGMSTAVSNAYDMAGWLGVKNNGLYNFPSSVRPVPLKMYIDGFPNNLAFCPLMKTMNKPAFMAIKQHSPDKPALIFVASRRQTRLTALDLIHLCGLEDNPRRFLNVHDEDELRFYLSQVTDDTLRLSLQFGIGLHHAGLVEKDRSISHRLFQENKIQILIATSTLAWGVNLPAHLVIIKGTQFFDSKIEGYRDMDLTDILQMMGRAGRPAYDTSGTAIVYTRENRKLFYKHFLNVGFPVESSLHKVLDDHLGAEITSGTVSNKQDALDFINWTFFFRRAHHNPTYYGIMEDTTEAGVNKFLSELVDKTLENLAESQCVKINGNEIQPTPFLSISSYYYISHKTIRQLLSQIYVNATFKDILKWLSMAEEFNELPVRGGEMIMNVEMSAQSRYPAEKTFIGDQELTMLDPHVKAFLLLQAYLSRVDLPIADYVQDTVSVLDQSLRILQAYVDVAAELGYFPVVMTIIKVMQCIKQGYWYEDDPVGILPGCNLRRRSDVEFSEQGFLLEKANSKQGGLTLESIGRMGSKKLLSVMDQLQVLEEERRQFLHVCQRLPVLDHIEITEGCGTLEISAKHFSRKTNRNYEVYCDAFPKMQKELWFVVAYRDQELLMLKRCQPKTRNKETVIACDLMIPEELEGTNLDILIVNDAIDLSYRCEHQVSSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9Y3</accession>
    <name>A0A8J2X9Y3_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ZYBA0S03-06348g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_06348g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X9Y3"/>
    <dbReference type="OrthoDB" id="9974421at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000095">
        <property type="entry name" value="Triglyceride lipase-cholesterol esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="8">
        <location>
            <begin position="103"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="474" mass="54696" checksum="9D0F497D1860006A" modified="2022-05-25" version="1">MRLSFTDTTIICLVYLETLSALLLRLVPQRVIRIVTWVINQFEAVAETEVEFQLRLAPTIHEMCDVFDVEVEDHLVRTEDDYVLLLHRIPPKPGTANGKVAYLHHGLMMCSDIWVCNIRRNNNLPFVLHDLGFDVWMGNNRGNKYSSTHLSRQPKSRRFWDFSIDEFAFFDIPNSIEFVLDYTQMSQLICIGFSQGSAQMFAAFSLNKALNTKVSHFVAISPAMTPHGLHNKVVDKLAKLSPRLMYLLFGNRILLPSAVMWQRTLHPKIFNWIIDFGNSQLFNWRAPNIRPAQKMVSYAKLYSTTSVKSVVHWFQILHAHRFQMFEESDGILNSLNKPYRIAHFPTKTNIRIPILLIYGGSDSLVNIEVMRQNLPAKGVFDAKVENHEHLDLIWGQDVETLVISKAIKFIDFFDNSLALCEGPAISYPKDDDDSLSLKDTSSFRDDTFEKYDRQSINNMSFKDLNLPSWQDKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XA10</accession>
    <name>A0A8J2XA10_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 20">Autophagy-related protein 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">BN860_03180g</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Phospholipid scramblase involved in autophagy. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate)(out)</text>
            <dbReference type="Rhea" id="RHEA:67920"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)</text>
            <dbReference type="Rhea" id="RHEA:38571"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:38895"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 20">Preautophagosomal structure membrane</location>
            <topology evidence="3 20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 20">Belongs to the ATG9 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316463">
        <property type="protein sequence ID" value="CDF91255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XA10"/>
    <dbReference type="OrthoDB" id="2020634at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007241">
        <property type="entry name" value="Autophagy-rel_prot_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038">
        <property type="entry name" value="APG9 AUTOPHAGY 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038:SF10">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04109">
        <property type="entry name" value="ATG9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 20" id="KW-0072">Autophagy</keyword>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13 20" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 20" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="298"/>
            <end position="324"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="356"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="514"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="599"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="637"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="703"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="760"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="885"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="129"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="21">
        <location>
            <begin position="942"/>
            <end position="952"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004439"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="RuleBase" id="RU364027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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            <dbReference type="EMBL" id="CDF91255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="968" mass="111336" checksum="3D0E1C2BFF6D5A00" modified="2022-05-25" version="1">MDKSKPSSQSQGRDTFLSRVFGLQSDDVTTSVHPDEMSQFREHDNQQESNLSGVHSDLASDSSALDEEGVRIPESDQGTTSEEEEEEDNESVTEDVDLNDNSALLTFDHKNNNGLGMEDSMPKVGQLSSEEEADGGSGAEEDEEEEEEADDEDRPLFSRAAGPKSNTQPPRQNHSMLFQRILKHNKQKDHQKKLFTSSRDTNHGSRRNRDFGVEMVDGGMRNSFLPGSDNYAGKKFNPPAFKLKRPNILNNISVLNNTPVNRVHALSPKERALWKWANVENLDIFLQDVYNYYLGSGYFCIVLLKILNLATLMFVVLISTYMGYCIDYSKVPYSHSISDITINQCYKTSITGFTKLLLWIFYIFVVLKIAQLYFDIQNLNEIHNFYSYLLNISDKELQSIPWQNVIQQIMFLKDQNALTANVVEVKAKNRIDAHDVANRIMRKENYLIALYNNDVLDLSLPIPLCRTSPLTKTLEWNINLCIMGYAFNENGFIKQCFLKPSQHEYVREELQKRFMLAGFLNIILSPFLVSYFVLLYFFRYFNEYKTSPGSLGARQYTPMAEWKFREYNELYHLFQKRIGLSTYLADRYINQFPKEGVNLILKFIAFVTGSFVAILAIFTVLDPENFLNFEITHEKTALFYITILGTIWTVCRGSISDEYNVFDPDETIQELAVYTHYLPEEWEGRYHTEDVKNEFCKLYNLKIIILVRELASLIITPFILWFSLPRSADKIVEFFRETSVYVDGLGYVCKYAMFEINQGPDSKNSKRKGSRRDSKNTRSPTRIADLPGGKKSIIHNNTSDGPFPSDGSDVEDENHELAVNKMMQSYMYFMDDYENSENALGKHQLPRRTFEEPMEGVRNLSNSYSWKKQFQPGQKPELFRIGRHALEVNKPHQGKSRRRSETSTHKRKKRGTPFTDDIDKDVEESFISSVPLRQYESSEAGKTGGKPNGSGGVLSLVKEYYKKSDIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XAC1</accession>
    <name>A0A8J2XAC1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ZYBA0S03-12948g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_12948g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="39175at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TF_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47338:SF5">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47338">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF89259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="580" mass="66932" checksum="15D4E79A1E7CEAB9" modified="2022-05-25" version="1">MRVSIACRSCRLKKTKCIHDGKAPCHSCRVRGKESTCILHSTQEFRKLRKTNIISQGKREESEIHIPDAIIKSSIEFAFQHYPELFFLSSLRYQEVVENLDELVILGVCVLSAPFTSYFTAQSRKLLASRLEVEVFGKIDGDPSMNLLSILQVSILVLLLNWQNRKMHKGYLLGGFAERAFLLLSEQDYRHKTYLEKEFMIRTLWSYQLITISLRKGSLEEYKTRLCKFPLPQENSEFLFRHASEIYHLKDFKSATTHNIFALFIAVTELWADCSSWVIQGGRQHFLEAPWDETSEWYKLDKRLNLFKSQLGPKESLNIQSMDAFFSLGQGTLYCCTHLALLTSKILIHRNYMPFIPPDNEVAKGPTDPSPALRKPPRNWWSHSARTVFEYAREAALIFNEFKKRNVYCCSTFAGFDALTCASTLFYIDSFPSYDPGFKDAALYYGYCVDFLKYYKNNWELGSYYYQFLVQTVAMFKAASDNKLTTLSISVFENMKDELVDVANVAAPSTGTKRIQIENLIDTTDVLTHCPTTVSGNFPNTTGEGLETWMNQKNREKLCSLFGWENEWPAFDNINEYWGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XAG6</accession>
    <name>A0A8J2XAG6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S11-01794g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_01794g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316464">
        <property type="protein sequence ID" value="CDF91390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XAG6"/>
    <dbReference type="OrthoDB" id="10262287at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043155">
        <property type="entry name" value="VPS33_dom3b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF91390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="671" mass="76413" checksum="582B16015B28AFEA" modified="2022-05-25" version="1">MSQNWNTRKFAKQLGKSFFKILNDISDNEQILVVQKEVLPVINALFTFTQLTESTPARKIVLISEQLGNEVSEILSTFPGMDLVFVIDARLDLAIPRPLRDVAKTLKLPVINVVFCSWETQSCNALAHVNRARDTRIPHFIESQLETSGQVRLMSWNMLPFPQVDDNVLVANVLYNSEKQNMYSPSESFVQTATRSILVDNMVNCLHALLNQTETTITNVVAMGAESWKLTQALRQRVEAEEDSEKNFIKETLYGDKYSGLETDLLVVERDMDPMTPLLTQLTYAGLVDDLYEFTPDAKTRQRKELSLKYAEDEVWQDLKFLNFGDLGSKLNVLAKNSDERYASRPKSDNLGELKQFVDAIPELQESRKLINKHIDLSAGILKKVENEQESQFNRILELEQNMLSGVLDNRGSCDNILDLIYEGQLDATRVVRLACLLSLCRNGLRDKDYELMKQELVDAFGIEICFQLERLASLGLFTSKSLLTHQNFSLWRKEYRNISYWLDTLPPAEDRQDSAKGEPRDASFAYCGVVPLSTRLIQMVYDRTVLSKHYNSQQPFIISRQPNIAKTEELISQIYGSPDCIHQESWMSALRKNKRRVTIGQPDPGTSDIVLLVFLGGVTMGEIATLKFLQDKLRQKEIYKRFIIVSDGVTNGNRITNSGIHPSMRVQKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XAN6</accession>
    <name>A0A8J2XAN6_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ZYBA0S11-01860g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN860_01860g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316464">
        <property type="protein sequence ID" value="CDF91393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XAN6"/>
    <dbReference type="OrthoDB" id="64767at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055087">
        <property type="term" value="C:Ski complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070478">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000354">
        <property type="entry name" value="ATP-dependent RNA helicase SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000987">
        <property type="entry name" value="DEAD/DEAH box RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.30:FF:000001">
        <property type="entry name" value="Ski2 ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048392">
        <property type="entry name" value="MTR4-like_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016438">
        <property type="entry name" value="SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012961">
        <property type="entry name" value="Ski2/MTR4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048727">
        <property type="entry name" value="Ski2_beta-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040801">
        <property type="entry name" value="Ski2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE SUPV3L1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08148">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21408">
        <property type="entry name" value="MTR4-like_stalk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21409">
        <property type="entry name" value="Ski2_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17911">
        <property type="entry name" value="Ski2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005198">
        <property type="entry name" value="Antiviral_helicase_SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01142">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="313"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="574"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="517"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="533"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CDF91393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1250" mass="141990" checksum="CBFF1254C493CAD9" modified="2022-05-25" version="1">MGLDVAELYEAIREATEDDVEIFEDKLSTFEPQGNDQDVQERFLQPSHVLPWSLLDLVQQFPETKETDYRDFITVPDPVNKTSFHLKRRGILGHVSGYEEEVNLKEVANANAANSLSLNREVNSHGNAVRGSTAQLPFMPGGVSMAAVQSAQGTSSVAAATKLLHKDDQGLFDVPRGFERGIEPSDAFTNPQLDGIEELQSLDKEDNELDVQREFEKQQRDQDDIVEKSTARQILGEDQELTTVTEGNTDIDDLLPMGIDFARNVVKSKGPLRPKEWAHVVDLHHKIDNFEELVPNPARTWPFELDTFQKEAVYHLEQGDSVFVAAHTSAGKTVVAEYAIAMAKRNMTKTIYTSPIKALSNQKFRDFKETFEDVGVGLITGDVQINPEAGCLIMTTEILRSMLYRGADLIRDVEFVIFDEVHYVNDQDRGVVWEEVIIMLPQHVKFILLSATVPNTFEFANWIGRTKQKNIYVISTAKRPVPLEINIWAKNELIPVINPNKVFLEANFKKHKELLDGKSSQSGVNGKATASRGGRGGSSVRGVGRGGRGGSRGAGAVGSNKSKFFKRSGPNKKTWPELVNYLRSKELLPAVVFVFSKKRCEEYADWLEGVNFCGGRERSQIHMFIENSVTRLKKEDRELPQIIKIKSLLERGIAVHHGGLLPIVKELIEMVFAKGLVRVLFATETFAMGLNLPTRTVVFSEIEKHDGSGLRHLNPGEFTQMAGRAGRRGLDKTGTVIIMTYTDPLPAATFKEVTLGVPTKLESQFRLTYNMILNLLRIEALKVEEMIKYSFSENSKQTLLPEHEQKIKELKIKLQEVKTYDDCPYCKQDLDQFLNSAIRLKECTAKMMEELVKTDKVFQVLRMGRLLLFRDQDDNLKLGFVFRVHMKKSSVIVMTFTEPNTLDSGEPNHLPYMTNMGAYTKRNFETFKAIDFFMEEVPITSIELTTAYSIKVSFVDIMKKEPEALKRFDEEVRIILKIAQRFRESTSEKQGSLKVHQYVLERNNIQKELSSFHALECPDLAKHFLPKYNAFLIEREIKDLYHLMSDQNLNLLPDYEKRLAVLKDANFIDQNNNVLLKGRVACEINSGYELVITELILDNFLGDFEPEEIVALLSAFVYEGRTREEEPPVATPRLAKGKERIEEIYSQMLEVIVNHQVPLTQEESEFLEKKRFAMMNVVYEWARGLSFKEIMDISVEPEGTVVRVITWLDEICREVKTASIIIGNANLHMKMSQAQELIKRDIVFAASLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XAU5</accession>
    <name>A0A8J2XAU5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S04-10000g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_10000g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XAU5"/>
    <dbReference type="OrthoDB" id="426293at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051020">
        <property type="term" value="F:GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15473">
        <property type="entry name" value="Myo5p-like_CBD_DIL_ANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037986">
        <property type="entry name" value="Myo5p-like_CBD_DIL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052072">
        <property type="entry name" value="Vascular_dev_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027:SF6">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN YPR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dilute" evidence="2">
        <location>
            <begin position="289"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="720"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="722"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="800" mass="91824" checksum="ABF2ABC7CF35F117" modified="2022-05-25" version="1">MSVLDNDIWGERPEATHESDKTAQAKATLTEILSQENRDDEDPWTSLIHLIADYEPGNEQLMTFEGLLGQIGDQVNNKSRTGLALIHYVIIYDHARYVELLHRYGLNLNLLDDVVGFSPLMWGFHLNRQDCCVELFSYIDELDFSLKNKDGLSAWDIVLPESPFNEFLDQNNIFQYKNTRDSPAAIGDLASEHSMDMQMAGMSLEGSNGVSGGYGTSDGFDFDRLAKDQYFEFSDFDVPQILDLLASLPQKYPHVTTYPAALLFQCIRYADHKLESPSLVESLVHLSLTRILSNFSSDLVPNEEEATGDIVIQSYWFSAISFLYYYLCRDEGFFKRHPQILQDLINGMHSLMIELTSSIHSRMVPLMEPALLSYTTIEDVKQTMYKKDWNIFKKKKPVRHQNAGDKGKGVPFYDGEMLKHLYPPPLEEQMKLSPMKLVQIFGALAYVLELHQIHPLLRQQFLSTIINWFSATFFNKLMRDKKKKYLSRARAIQIRLNLSSLDNWIKNNDRTVPKPVLIDDFMWQRFPYTLASDLGDIDLSHPPIKNVATYKPLDTDVENTDVYDSTNSLFYYQSLHHIAQVHMAPVNQLLQWLQVATSLVNEESLDETMELLSRLTPIQLLKSMERYSYEVNEHKFKTPLRKKLSTIVKTQNSKEEPYLPERQLPVLALPTIAELTDTYTYSVDSESYLPVLSVDIQDAMYDIHEENVRLRRDEAIAREQRWEEEEDENEDEHSSVNDNGSEEAAAKNNSTNSLALKDNGGVIKDGNDVGDAYFKEMTAPSAIAHRPTWADNPEIEANPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T0A5</accession>
    <name>A0A8J2T0A5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">BN860_14114g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN860_14114g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T0A5"/>
    <dbReference type="OrthoDB" id="4065753at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDF87771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="468" mass="55014" checksum="6109B3BCEF122662" modified="2022-05-25" version="1">MHNRMVFNESQFGYSILFRYFDEEVQTIAAKFRSASKYSERLYRRVVLLILTYCPQECTRTIFGKNCKTRLKMENLMNNEMLKYVELDFHANSKVMAKALVKCTSSSMKVFEYKIFLLLANKGIFAYLSYFDGEYLKSFTEFRECLQFIYYIKQFGPEKCQIYSFLDDCERMFSILCATSLMAEIEGRRNSCVLSRFKDFKLDEVSVLQGLFFTVIDCTDPSLMTAANDLDRYVLGRFFESLGEIERSIAIYRGSLAEYECKPNVNDLALRVQKDHLGGMIRRYILAMTIKLSDDPAIIRCLYSILEGLVLYGGVHMEVIWFFYSLKVYYEVAIESSEEFLDKDLTKECVMMLNLVCQKWQQIRKKYKPRTVQLPQILLKSMFGDFVIVDEVFEENNPFEHHHELSVILSSIKLKSKHKWFGEVNAFEAYSDSMWKTGIEWVDFWKDCYLDNHSELPVELLVILEGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T138</accession>
    <name>A0A8J2T138_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BN860_06304g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN860_06304g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T138"/>
    <dbReference type="OrthoDB" id="2248014at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02662">
        <property type="entry name" value="Peptidase_C19F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF827">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="3">
        <location>
            <begin position="73"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="551"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="570"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="617"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="629"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="657"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="706"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CDF87435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="795" mass="88071" checksum="BB61E9784F7175FC" modified="2022-05-25" version="1">MPQVVYVVSRGSSNVNGYWLNSFLHSNNKGLRYITYGVLTVLSIYVLAPSVTYLIFGENMFSSGSKRSDKTTTGLINNRNDCFANSSVQALSALPKLTLYLNDILKQATQVAKDLKGDDVGPREEPNQPGSAESRQTATANNDPGLSRKQIETLATPKAELEQNPLDMHMANMSINRPGLSALKSNATLTTLKDNDNGGDETPEMSLHLADESGDTPVDDSRLPKLPMHRSLASMLEQLQRTITSTRYLSIWPLLHCLEVIFNARISSGQNDAHELTQVILETLQKENIKLREFVNNHNISMRIPEIPFRGKTADHLVCTACRNSSKVKTHQFIMYPLTVPQALQSKLADMVSDNQTETIEGYSCLTCKVSKILENERGRDTSKYPVTEQNILKSLSNALPNMLINDDVSEDLHNYIDGYDKDGCAVGKLKSTIVKKTVVVEAPPILLIHLSRSIFNGATYSRNSCAVNFDEILQLQEQVIENNRCVGINTVGYRLKAIVKHSGSHSQGHYQCYRHKPDFVKDIDTHAVINRTPVIDASLVNIQDPQASKEMAATEMMEPPSRQLGDSHTNLSSTSLSNSSTSPRVDNPENSEDRTFDQGDSAKMSRKPSAIKKITNFLSGKTSLGNQSSDNTETSDSETGGRSRKLTASRSVEGNSRNPSSIRKSSSRSRNPSAIRTSENMSRTSSSTSRSRASSVSSFHRNRSRANSISSDRTPSDFSETTSSEENLGTTASETDDNSANPTARKRHLKKIKSVTKYPYWLISDTAVHEAKPQEVLNETKYVYMLFYERFDTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2S1</accession>
    <name>A0A8J2T2S1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Replication protein A subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_08262g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">As part of the replication protein A (RPA/RP-A), a single-stranded DNA-binding heterotrimeric complex, may play an essential role in DNA replication, recombination and repair. Binds and stabilizes single-stranded DNA intermediates, preventing complementary DNA reannealing and recruiting different proteins involved in DNA metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the heterotrimeric canonical replication protein A complex (RPA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2S1"/>
    <dbReference type="OrthoDB" id="1751331at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04474">
        <property type="entry name" value="RPA1_DBD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04475">
        <property type="entry name" value="RPA1_DBD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04476">
        <property type="entry name" value="RPA1_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04477">
        <property type="entry name" value="RPA1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000041">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000064">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000090">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047192">
        <property type="entry name" value="Euk_RPA1_DBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007199">
        <property type="entry name" value="Rep_factor-A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
        <property type="entry name" value="REPA_OB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004591">
        <property type="entry name" value="Rfa1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00617">
        <property type="entry name" value="rpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Replication protein A OB" evidence="12">
        <location>
            <begin position="297"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Replication factor A C-terminal" evidence="11">
        <location>
            <begin position="457"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="134"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="157"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF87520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="612" mass="68851" checksum="D2D0C8D5AAECD4AE" modified="2022-05-25" version="1">MSDVQLSKGDFRTIFTSQQRYDNPTNGVYQIYNIRKSESGNSNRKNLIMISDGVYHMKALLRNQAASKFQSLELQRGDVICVKVAEPAIVRERKKYVLLVDDFEVVKTGLPLVNQASEFLDSYFVSHPNETLRDDAHSSTNAAAPTASLATSSTGAQPDSSNQHKFSASSQKSKPIFAIEQLSPYQNIWTIKARVSYKGDIKTWHNQKGDGKLLNLNFLDTSGEIRATAFNDMATKFNEMLQEGKVYFVSKARLQPAKPQFTNLSHPYELSLDRETVIEECHDETDVPKTHFSFIKLNTVENQEANSTVDVLGIIQRVNPHYELISRAGKKFDRRDITIVDDSGYSIAVGLWNQQAIDFNFPEGSVIALKGVRVTDFGGKSLSMGFTSTMIPNPEIPEAYSLKGWYDSTGHSMQYHSLKQEPGAGASGGNLMKYIGERITIARAQAENLGRSEKGDFFSVKAAISFLKVDNFAYPACSNENCNKKVIEQPDGTWRCEKCDTNNASPDWRYMLTTSIVDETGQLWLTLFNDQAKQLLGIDANELMSLKENDPDAFTKITQSIQMNQYNFRIRAREDNYNEQSRIRYTLSNLHTLNYKAEADFLAGELTKDLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T2W7</accession>
    <name>A0A8J2T2W7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 18">3-isopropylmalate dehydratase</fullName>
            <ecNumber evidence="6 18">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 18">Alpha-IPM isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17 18">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">BN860_08614g</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 18">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 18">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="6 18"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T2W7"/>
    <dbReference type="OrthoDB" id="2279155at2759"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009316">
        <property type="term" value="C:3-isopropylmalate dehydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000006">
        <property type="entry name" value="3-isopropylmalate dehydratase large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000007">
        <property type="entry name" value="3-isopropylmalate dehydratase large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000003">
        <property type="entry name" value="3-isopropylmalate dehydratase small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01026">
        <property type="entry name" value="LeuC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01031">
        <property type="entry name" value="LeuD_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004430">
        <property type="entry name" value="3-IsopropMal_deHydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004431">
        <property type="entry name" value="3-IsopropMal_deHydase_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012235">
        <property type="entry name" value="3-IsopropMal_deHydtase_ssu/lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050067">
        <property type="entry name" value="IPM_dehydratase_rel_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00170">
        <property type="entry name" value="leuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00171">
        <property type="entry name" value="leuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002458">
        <property type="entry name" value="PRK01641.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004016">
        <property type="entry name" value="PRK05478.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009116">
        <property type="entry name" value="PRK12466.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822:SF9">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001418">
        <property type="entry name" value="ACN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10 18" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="15 18" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 18" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="14 18" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="20">
        <location>
            <begin position="14"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="21">
        <location>
            <begin position="552"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="513"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="516"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR001418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CDF87535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="780" mass="85326" checksum="20CAF5EB7114C75A" modified="2022-05-25" version="1">MSEVSSRGPRTLYDKVFDAHIVKNDDTGSCLLYIDRHLVHEVTSPQAFEGLRNAGRKVRRTDCTLATVDHNIPTASRKNFKSIGTFIDQPDSRLQVETLEQNVKDFGVTYFGMTESRQGIVHVIGPEQGFTLPGTTVVCGDSHTSTHGAFGSLAFGIGTSEVEHVLATQTLIQSKSKNMRIMVEGDLSPGITSKDLILHIIGVIGTAGGTGCVMEFAGKAIENLSMEARMSMCNMSIEAGARAGMIKPDETTFNYVKNKPLAPKGAEWEKAVAYWKTLHTDEGAKFDHEVIIKAADIVPTITWGTSPQDALPITACVPDPSEVTDPIRKAGMERALKYIGLTPKTPLKDVKIDKVFIGSCTNSRIEDLRNAAAVVEGHKIAGNIKRAMVVPGSGLVKSQAEKEGLDKIFEAAGFEWREAGCSMCLGMNPDILNPEERCASTSNRNFEGRQGAKSRTHLMSPAMAAAAGIAGHFVDIREFEYKDGDVPKISVGQEEEDKQLQEAVYAHENEVLQPDEVSEEKAEEEVDDIPLDEPETKGAERNGTALPAGLPPFLTLEGIAAPLDKANVDTDAIIPKQFLKTIKRTGLSAGLFYEWRFTKDSQGKDQPTDFVLNQEPYKNAKILVVTGDNFGCGSSREHAPWALNDFGIKCIIAPSFGDIFYNNSFKNGMLPIRINQQVIVDKLLPLAKAGKHLVVDLPKQQIRSSDGTVLVDHFDVEEFRKHCLVNGLDDIGLTLQSESFISKYEAIRRDKYSFLEGGSKLIKPVKGTKKSPFGVVAQEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3I4</accession>
    <name>A0A8J2T3I4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BN860_13520g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_13520g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3I4"/>
    <dbReference type="OrthoDB" id="4070347at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF87746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="463" mass="52179" checksum="1BE22548AA60444E" modified="2022-05-25" version="1">MSNPIPQLVNISHALQSQTIEKIRHDIRDFHEASKLKPEQVEAINHYISSLKAAFAQFTKDNEHVERSDAGVTAVDLQLYSGLKSMYTDYLNQLSTIKKEKVLGDEEVGKDRPMDYIVEELPYLQPMERKEYIEGLIMNDINANTLHKSERFLNGMINLCVLDSTVTENLRCYVTLMRKVGYTKEELKTFLPKGLAALPWLSLGFVDKSLDNTSRKLSLTKETTPPLLEAQAGSTDSGGDEEPKKKISFSRYLKKGDGSENSKRKSSVREEDVRSFKKAKVSGQLPSILKSSNSDKKPINNIKFADDSHLLRVYGEGLPSEGLQLSPEKLKKVLKPFKEGEPREIVYNELFKGKAAELEIEFDLSPEESDIVETKGGPVPCETTVPLKYRLNFSNLHPDLGNKPVREAVMVEDHFDTSKSLKNKGPLIARAFGKNSLLLRKDRGGLPYKRVPEVTPNDYPPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T3J7</accession>
    <name>A0A8J2T3J7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol-4-phosphate 5-kinase</fullName>
            <ecNumber evidence="2">2.7.1.68</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">1-phosphatidylinositol 4-phosphate kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Diphosphoinositide kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">PIP5K</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_13850g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14425"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.68"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.1.68" evidence="2"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T3J7"/>
    <dbReference type="OrthoDB" id="20783at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17303">
        <property type="entry name" value="PIPKc_PIP5K_yeast_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000009">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase its3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF8">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 5-PHOSPHATE 4-KINASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="13">
        <location>
            <begin position="337"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="214"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="39"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00078403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00080374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF87761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="746" mass="85299" checksum="BC8AA91E8F22251B" modified="2022-05-25" version="1">MNGRPAVASSASANLLHTRGSPRRRGKEKRCRHCEHQHSVSYHSSSNSESNIDSPRLSDGERSSSTPTSIDESAAAVAQKQPNEKNRAAPIVDTKQMQALSISTPVNTHSQTEVPLRSPPQLLGKSNQEDEREQEQTQEQTQEAMPRNEARHSYQRRFFDGKEIHLPQTQQQQQLESHAKEELLLDDESLVHTDSYIHGEENDAVLLNKRISRASTRKSRQQQQHEQQVRKSMPFLAHKHSQILPLDDEKKPSKHVSNKSMPSLAVHAPKSSGLNKMASASYTSLPPNAQGGNTGLLKRSESATAEIKKMRVSLLHKREMRKKRKSFLMDDDRVLIGNRVSEGHVNFIIAYNMLTGIRVAVSRCSGIMKPLTPADFKFTKKLAFDYHGNELTPSSQYAFKFKDYCPEVFRELRCLFGLDPADYLVSLTSKYILSELNSPGKSGSFFYFSRDYKYIIKTIHHAEHIHLRRHLYEYYMHVKENPDTLVCQFYGLHRVKMPISFQNKVKHRKIYFLVMNNLFPPHLSMHATYDLKGSTWGRLTKVDPQLAHDENYTPVLKDLNFLEREERIKFGPLKKKKFLEQLRKDALFLAKLNTMDYSLLLGIHDMSKSKDEELATGETASLDGTHALAPSTTLGTTSMDTPTAIRNGRVIPHYFKQFEGGIRSSDAFNNDTNLVYYIGIIDCLTNYSIVKKLETFWRGLSHDLRVVSATPPRDYAERFYEFIEEAVDPLPPKRYKDDPTATNYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4B1</accession>
    <name>A0A8J2T4B1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="10">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_10528g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="10"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4B1"/>
    <dbReference type="OrthoDB" id="4310724at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17946">
        <property type="entry name" value="DEADc_DDX24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="14">
        <location>
            <begin position="230"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="261"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="500"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="94"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="8">
        <location>
            <begin position="230"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="13"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="170"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF87617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="798" mass="89880" checksum="282B5878A34B8351" modified="2022-05-25" version="1">MSNSSMKATKSNMVKKHSKVAKGKSNLSKKKNTKPKIKGKSQRKTPKSVIVAPSELKWKKVDIPDSLDDYGGFYGLEEIDGVDVRVVNGKAEFITRDESVLKNEGEHTKQNVEESQDYHNQNTHTQHLPVAIEDEQNQNEELKREEDLDNSISESRHDGAQDNQDSQSQETEKKADDSDDEIQHKNSPLNEIVQDRAGKNQEGEQDQELQQNTFNADLDLDDLTVPELPDWSEVAPLSFTVLLGLSQQGFTKPTDIQRAVLPAALRNEDIMGKAATGSGKTLAYGIPILESFVKEKDLSKPIALIFTPTRELAQQVTEYLKKLGQLVVKESPFAILSLTGGLSIQKQERLLNYDNSASIVVATPGRFLELIEKNDELVSRFAQIKTLVLDEADRLLQDGHFEEFERILKLLGSARKTYDKIGWQTMIFSATFSMTLFSKLTTTSWKSLKNNEDELEQVLKHLMQKIHFKSKPLIIDSNSEEKVKSEIKESLIECGPLERDLYSYYFLTVFPGTTLIFCNSIDSVKKLCAYFNNLKISAFQIHSSMTQKNRLVNLERYLQQSTKNSTEGKSTVLVASDVAARGLDIPGIKHVIHYHLPRTADTYIHRSGRTARAGHEGVSVMICSPEEAMGPLRKLRKMLADKGGEGGLGAHKKWQKEVPLLPIEPDIVRELRERSVLANELADHQLASASLNKDSNWMKQAAQDLGVDIDSDDEFNDPTINKKRKKTLSKVEAKQVKSRLNSLLNRPIRKDRRQKYLTGGFTNLADNLVKKRGHESIIGHEKVGALETLKSNKKRKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4E9</accession>
    <name>A0A8J2T4E9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_14444g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_14444g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4E9"/>
    <dbReference type="OrthoDB" id="4068181at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13746">
        <property type="entry name" value="Sir4p-SID_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.730">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031556">
        <property type="entry name" value="SIR4_SID"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16991">
        <property type="entry name" value="SIR4_SID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90242">
        <property type="entry name" value="Dimerization motif of sir4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sir4 SID" evidence="2">
        <location>
            <begin position="374"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="619"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="742"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="630"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="745"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="761" mass="85804" checksum="3DFCE67B5C79EEF0" modified="2022-05-25" version="1">MNKDQEGPRNIKLVRYTHPGPRAWKDGLERSKSLPVREKRKGDGRLIDMLKSRSAKFHNITVGRGPTTTTSKGDHAIGTKQHEPFFLPTRTSNPLRTSQSTVQSINLNGALSTEHSTGPSFTASVKEMLMEHRPNFGSLHTQGENASQDISRGNNSPQRTDDLMQKELLQLQQKSTQSENSKEVNANQLEKETKDLTKRPSQVEEGQKQKTPPAETSFKPGEANQAKASVWGPGERKDHDSRALPQAGKGALQHVDTTKPVEINSISSAQEGSLLVQKEPQSLQLLPYMKRPESTRSNLLRLFSRVDEEIHSADSASNNRILKLLEGNTGQVDPPSDRPEDTRSLQGTVVAFEGELGAIVDSGRSKLQVVYLSDDDEIPPKRRKIVPPGSGVLKRTSPRRLMPTLTDPSAEKRKNDREVAIDVLVKLKDKRTYERQIIAELVEKGVPEHRALEGNPGASVTNDVCSVNNYETSHAYYYQQLDKRSKLSWIPLHSAQTNNRQQPKEIWRNELQASLISCCVFFYSVEPSNLPEGQKRAMEHQITQLKRVLQTRFLVKLADSLDKKVDVVILYGQNEKLLSELVQKRLKNMPFRVWSLQRALDFLDDVDVDLEEWGIPRDAQDREPIVEAPSENTSSTPEQKSLTESEADSEDETLDDADQYVQGLLQQASDALDERDCQLKAARRIILALSKNVMSQELQVTSLTGQLKDSQRKLQNQENLTEQYRLCMAQQELEMAQLRSQLSKQRSKKLKGNHNHVSKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4F4</accession>
    <name>A0A8J2T4F4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Riboflavin kinase</fullName>
            <ecNumber evidence="4">2.7.1.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Flavin mononucleotide kinase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_14818g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) coenzyme.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the flavokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.26" evidence="4"/>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4F4"/>
    <dbReference type="OrthoDB" id="276388at2759"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00406"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023468">
        <property type="entry name" value="Riboflavin_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015865">
        <property type="entry name" value="Riboflavin_kinase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22749:SF6">
        <property type="entry name" value="RIBOFLAVIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22749">
        <property type="entry name" value="RIBOFLAVIN KINASE/FMN ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01687">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00904">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0288">FMN</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Riboflavin kinase" evidence="12">
        <location>
            <begin position="24"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF87802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="188" mass="21390" checksum="1F063E34B0C6DCA2" modified="2022-05-25" version="1">MEPTSNEWPIPENPEPPFPIVTDFCDIVFGFGRGSAELGIPTANVPLQQLPKETQDLKLGVYFGFAILKPMSHEETTAVRKDGRKVAYNYGKYLSKSSGDLDILPVVLSVGKNPFYHNVIKTVELHILHHFESDFYGAQVKFNLLGRIRPERNYNTKEALINDINHDKLVAKEVLSKENYLKYERQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4F8</accession>
    <name>A0A8J2T4F8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">ATP synthase subunit d, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_19174g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ATPase d subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4F8"/>
    <dbReference type="OrthoDB" id="35799at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008689">
        <property type="entry name" value="ATP_synth_F0_dsu_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036228">
        <property type="entry name" value="ATP_synth_F0_dsu_sf_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12700">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT D, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05873">
        <property type="entry name" value="Mt_ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005514">
        <property type="entry name" value="ATPase_F0_D_mt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161065">
        <property type="entry name" value="ATP synthase D chain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="5" id="KW-0138">CF(0)</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 12" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR005514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF87993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="174" mass="19464" checksum="0AB9E860F5B823E7" modified="2022-05-25" version="1">MSLAKSAASKLDWAKVIKTLKLTGKTAAELSSFKKRNDEARRVLFELKSQPVDVDFSHYRSILKNTAVVDKIEQSVKAFQPATVDTSKQLSTIEAFESQALSNAKETEAIVAKELKDLKETLKNIESARPFEELTVDELAQAKPEIDAKVEEMVKKGKWDVPGYKEKFGDLTVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T4Z5</accession>
    <name>A0A8J2T4Z5_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription initiation factor IIF subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_08174g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TFIIF alpha subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T4Z5"/>
    <dbReference type="OrthoDB" id="76676at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001096">
        <property type="term" value="F:TFIIF-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008851">
        <property type="entry name" value="TFIIF-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13011:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13011">
        <property type="entry name" value="TFIIF-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05793">
        <property type="entry name" value="TFIIF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="29"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="143"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="392"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="576"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="41"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="401"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="433"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="458"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="469"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="510"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="536"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="671" mass="76181" checksum="F1CE302F75830C18" modified="2022-05-25" version="1">MASGRSGNGYSNVSPFIKPDRLRRNFLRAKMSRGGTPRPSVKKDDPEMQEAREKMMLHQDGLEDSSRNGRVKVKEESPEEFNEFPLRAVAREELDNMRTHLLKFQSKKGINPTKDFHLPIRLHRKDTRNLQFQLTRAEIVQRQKEISEYKNKQDQEKGGGKKREDGTPSEGIEKSQSPGSPPPVQATASTPEEPASVKSPDVKEENGSTSKPGDVPVITKLEEAGPASDPTKVGMVKYDGEEDVTEADTATADPLADVAPDGGGRAKRGNLRRKTRQLKVLDENAKKLRFEEFYPWVMEDFDGYNTWVGSYEAGNSDSYVLLSVEDDGSFTMIPADKVYKFTARNKYATLTIEEAEKRMDKKSGEVPRWLMKHLDDIGTTTTRYDRTKRRLKAVNDQRANEEDEHSDNSEVELDYDEEFADDEEAPIIDNDEKENKESEQRIKKEMLQANAMGLRDEEQPDDNEEDDLFNEKKIDEEGERIKRALQKTELAALYSSDEEEINPYLSESDVENKEQSEQVKKENSDEVPSSKKGSPKKNTGSTPVLNSHREPQVRVKSIKDCIIVLKAEKKVLMNFPQGDWNPGLAKRKREESTAENPESGASKKVKLENDITQLITEQDIIDAIGDGKVNVKDFSKAIRRKYPGPENKKLMVAIVKKLCRKVDNEHMELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T527</accession>
    <name>A0A8J2T527_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">BN860_05116g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_05116g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1678912at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16100">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15518">
        <property type="entry name" value="PHD_Ecm5p_Lid2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013637">
        <property type="entry name" value="Lys_sp_deMease-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF113">
        <property type="entry name" value="PROTEIN JUMONJI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08429">
        <property type="entry name" value="PLU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ARID" evidence="9">
        <location>
            <begin position="117"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="10">
        <location>
            <begin position="377"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="1145"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1297"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1329"/>
            <end position="1342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF88225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1342" mass="152871" checksum="B1DD5F9F47CD61D6" modified="2022-05-25" version="1">MKVEGDRKRKSNGRSMPVFDLSQVSIKKVRCPGVEETLFDQGTQLHYRLGAQSVPSFQLEQEFPDPVKFYEKVRPLGERYGAVKVRMVGERAGTAPDPEHFWFQTRTQHLRSAREQRREVLQFHRKLYECHWRLGHHLHKVPSIDKRTLDLYRLWECVQLRGGYHAVCQRKLWAQIGREMGYSGRIMSSLSTSLRSAYAKVLLDFESDPQNAYVPAQSTSSGIYSVVNSAEECSTLRDFKRVKGFSTNYSPQSTHLEAATVLHGATTLPGYDWEMWQTGMEIYDSSDQETKTPPVYNMKQYYDKSQVHLAEVASRCGEQLPLALDSREWEQEAFEKWYIAVLANPSLSTHVDTAVSLSSAAHNFGELSGSDPGEVGHVIRDLWNLDNLPLSHESMLRHLELDWGNFTHAAIDVGMLFSTKGWGMEPLDLPSLTYNHVGSTKIWYVVPPEHCRKFEQLLRDVNSAQKEVSEEEETGSNRGSDLNAEADLEFRRSDIYKSFLEINGHDYHSGSSRMKSEMLKLLSDEAQHSEEDFSFLPGDLQIAPHVFEKHGIKVFKITQECGTYIFRFPQSYTSTVDSSFCVSKRARFAPVSWLGRLSHTNEGTQAVSELPKFLPFQFLMNIVLSSEGSDEREAKTKGRKLLEPFIRKELLNREKLNQLQLDYVVVEDKFDYVSDLSLKAAGASKIVVDNNGDSISLRLDEFLQQVTAAGDGSLSLGGKELGGMHVCLHIYYSDSILLSALGEQVVHSQSAAVNDTITEKLDTLVNVNFKDRRIPVEHLKQLFSGQSTMEQSWRGLLMHVEALRKECCVMLDKLASSEDSLAAVFIGKGLNLVELQGVQDGVEVGTLHKLQLQLQTCSVDFPEMSKIFQLCEKVSNFQMQAEQALCDKNLQELERCYRMGFSCGIKSKYMGAVAFWICKLHWLRVYQETFVEHLKDPSEDPLNYSLDCMEWFLIYGTKYCGPNEVDKLKKVRSHIMDTRRTRDKISKIFKARKPKSKFLVKDVVSVLELINKERLPIDAELVKLLRSIIDSIQQAKSEMIPLQNLLSINQPFVDNLRDQIKENSLEALKLFSTFDGGFGDKRICMRDAIDNKYFSKLCKPCKLWLSSVSRLCGRGNLEKLVYSVSRCLDLELDTYPALQNDSNQNFYCFCRQDDIGGTMIECELCKEWYHVRCLRKDRWKLPEESAFICSICSNNDEVTHTIEFSSVADLIIDSLELKLVPDRDIVSSLFSIYGKMLIFQNRMQSELFKDGKVEAGVTTNKIKFFLRKAQGSKCSFGSLTKSLQDRCRAENVVSLDDSTKNESADSETQFSSIADEPVSQMLNQGNSGMRKETRREMKSATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T539</accession>
    <name>A0A8J2T539_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BN860_09648g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_09648g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T539"/>
    <dbReference type="OrthoDB" id="434160at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000171">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000004">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000020">
        <property type="entry name" value="Heat shock SSE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF4">
        <property type="entry name" value="HSC70CB, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="645"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="645"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="673"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF88411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="695" mass="77424" checksum="DE1EF8C3A9F1357F" modified="2022-05-25" version="1">MSTPFGLDLGNHSSVLAVARNRGIDIVVNEVSNRSTPTVVGFGQRNRYLGETGKNKQTSNVKNTVENLKRIIGLGYDDKDFPEEAKYFTSKLVKLDDGKVGARVKYAGKDTVFSAAQLAGMFFDKVKHTVISETKANISDVCLAVPAWYTEEQRYLVADAAKIAGLNPVRVVNDVTAAAVSYGVFKPDLPDADQKPRIVALVDIGHSTYTCSVIGFKKGEFKVLGTAWDKQFGGRNFDRAITQHFADEFKSKYKIDINENPKAYNRILAASEKLKKVLSANSAAPFNVESVMNDVDVSSQFTREELEELMTPLLKRVNGPIEKALAQAKLNVEDVDYVELIGGTSRIPALKDSISKIFNKQLSTTLNQDEAIAKGAAFICAIHSPTLRVKPFKFEDIHSYSVSYSWDKQVEDEDHLEVFPAGSSFPSTKMITLHRTGDFGMEAFYTNKNELPQDVPVHIANWDITGVQLPEGASSVPVKLKLRCDPSGLHIIEDAYTLEDITVKEEIPLPDDAPEGAEPEYKDVVKTVKKDNLSIVAHTFGLTQKELEADIQKEVEMCKQDKLVAETEDRKNALEEYIYTLRGKLDEEYAPFASEQEKSKLRDQLAKTEEWLYDDGYDSTKAKYIAKYEELASTGNIIRGRYLAKQEEKKQASRQKNEAAKMAELSEKLNAQRKSESEGKKSSKTDAEGDVQQLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T544</accession>
    <name>A0A8J2T544_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BN860_06062g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_06062g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T544"/>
    <dbReference type="OrthoDB" id="440128at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010491">
        <property type="entry name" value="PRP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06424">
        <property type="entry name" value="PRP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PRP1 splicing factor N-terminal" evidence="8">
        <location>
            <begin position="11"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="777"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="29"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="874" mass="100470" checksum="D3209B597A798477" modified="2022-05-25" version="1">MDLPSFLSQKPPPGYVPGVGRGATGFSTRGDKGGPLKVPKRFETHTSHDSLHEDKTRTAEHLEEEEANQIYSELDLKLSTRNRSKRHEERPGSSISEQFADLKRSLATVTEDQWLSIPSAGDYTRKRKRERLDEQLNRKTYAAPDTLLTPHVNLSKLTEERERILEKQLDASIFEGDNGAAIAEAQEYLNQLQAKSDLDDNEDDVKKMRLILQSYRHSAPKKPEGWIASARLEEKARRLRTAKSLIEEGCSQCPRSEDIWLESIRLHGPDNYRCKVLVAEGIRFNPQSLSLWLKAIDLENEVINKQRVVRRALQELPHCEELWKKAVKYSKEKEEVLKILQKAVELIPRSFDLCAALVQLQDYEDAKKTLKAATKSMPLELRVWILAAQIEESKGDNVSFEQLTELLTNGIKEMQKNNVKLSLRALLEEANSLLSDHHSHNTVEAFTCVALNEYAKQQSEAENTRTIAALPNSIIKILAFRFLLKLNPTKISTWASLKATCESTGKIAELYSTFDSLLFNAKQDYRILKEHPVLALMYSKELWKYGEDLPKSLNVLDRALEIVPFSLDIWLAKIKLLCVSARYDVAEITFKQALKNFENYKVSNVEKLYYKYVSFLRFQQHHEVAIEFLKQKCLSEFPKCYKFHLQLGQIYHSLGQLDNSRESYTEGTKYLPGCVLLWISLAQLEEEGLHRAIKARSVLDMAFMKNPENEYLFLAQAQLEARLGNTDQARFIVHQALQKHPHSPHLWAENIRLLPLKKANVKKTMFQDALKSTNNSYEILIEIGISFFEESQYGPALKWFERATKKNPAYGDSWVWLARCRKKLNNDIAAIKERVKELEPTHGVLWVKVSKNPKFQYLSPSQILDTLIFSEQVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T594</accession>
    <name>A0A8J2T594_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S03-12816g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_12816g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T594"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF89253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="334" mass="38562" checksum="174DB1DFFEB36FBD" modified="2022-05-25" version="1">MEKSGQLHSHTRILDDTRDSTEQNPPPYASFSGKRIMPVAYSLGKEGVMVFPSEEALSKYKHINYDRDPIDPHGLGLPLFKLIRKHRFLRLGKKPHYVIYKYLLLKNGAPAPQGSEVVVSDSTHTVYNVPFCYVYRKKHSKLTSYVFEFLAELRTRELELIDVQNEQRYCGSVGSYDLLWSSPDDEPDEYELHMDLENVMRMITNDLPSEKGKNKCKSEKAFPKEQQELSVVASYTLENRDYLPKKHPKYGDLILLEQSQVGNLETLGITSVPVVTQLLACHGLILQQLIEKVVYREEHQVERNKPAAQYAGKRDRERCYLTPRSEVWDSIFAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5F0</accession>
    <name>A0A8J2T5F0_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BN860_13080g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN860_13080g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5F0"/>
    <dbReference type="OrthoDB" id="6415790at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008521">
        <property type="term" value="F:acetyl-CoA transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035348">
        <property type="term" value="P:acetyl-CoA transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024371">
        <property type="entry name" value="AcetylCoA_trans_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004752">
        <property type="entry name" value="AmpG_permease/AT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778:SF9">
        <property type="entry name" value="ACETYL-COENZYME A TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 33 ACETYL-COA TRANSPORTER -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13000">
        <property type="entry name" value="Acatn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="14"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="385"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="415"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CDF88560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="556" mass="62096" checksum="019077F03A6A8684" modified="2022-05-25" version="1">MGKKNLAKKDLPQFYLLVVLYLLQGIPVGLAFGTVPFLLKSMAKETSFTTLGIFSIATYPYSLKILWSPIVDSIYNPKVGRRRTWIIPVQLLSGTMLLLLGCAVSRNLVFKGVDDAFHGRQSQGIHNLNILTLAWYFGFLVFLCATQDIAVDGWALTILSKDSLSYASTAQTVGMNFGYFLSFTVFISLNSSDFANKYIRTVPKEYGFVSLGGYMKFAGALYILVTIYVIFCTKERPLDHILPQAGSSATKKDDESKSVVEYGDGDVIGTSNTASIKYVYKCFGKILKLRAVQSLAFIHLTSKLAFQCNEAATNLKLLEKGFKKEDLAVTVLIDVPFEVIFGYYVAKWSSESSHHHNHPSHRQVLTGINRWVKFLVGEPGILTPWLWGFLGRLLAAALGSYVLKIFPQDAKISRTYFLIVVFQHLLGSFMSTVQFVGISAFHTRVADPVVGGTYMTLLNTLSNFGGTWPRMIIMSMINYFTVYECYVPNTNQVITYQGGSMEKCTDGWKGQASILRDGYYVTNGICLIIGTLLYFGYLKRKAQQLQTIPVDAWRCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5Q7</accession>
    <name>A0A8J2T5Q7_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL23</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">60S ribosomal protein L25</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_12442g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5Q7"/>
    <dbReference type="OrthoDB" id="1267328at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000035">
        <property type="entry name" value="60S ribosomal protein L23a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_uL23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001014">
        <property type="entry name" value="Ribosomal_uL23_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005633">
        <property type="entry name" value="Ribosomal_uL23_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011118">
        <property type="entry name" value="PRK14548.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03939">
        <property type="entry name" value="Ribosomal_L23eN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00050">
        <property type="entry name" value="RIBOSOMAL_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL23 N-terminal" evidence="10">
        <location>
            <begin position="3"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00075004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF87699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="142" mass="15961" checksum="EEF38A0757220E2F" modified="2022-05-25" version="1">MAPSAKANAAKKAVVRGSNPKKVLKVRSSPTFRRPKTLQLPRNPKYARKSVPHYERLDSFKTIEHPITSETAMKKVEDGNTLVFQVSLKANKHHIKKAIKELYEVDVLSVNTLVTTNGTKKAYIRLTADYDALDIANRIGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5S2</accession>
    <name>A0A8J2T5S2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA replication ATP-dependent helicase/nuclease DNA2</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">BN860_02828g</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18041">
        <property type="entry name" value="DEXXQc_DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000721">
        <property type="entry name" value="DNA replication ATP-dependent helicase/nuclease DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000789">
        <property type="entry name" value="DNA replication ATP-dependent helicase/nuclease DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026851">
        <property type="entry name" value="Dna2/JHS1_DEXXQ-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014808">
        <property type="entry name" value="DNA_replication_fac_Dna2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN SMUBP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08696">
        <property type="entry name" value="Dna2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="19" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="18" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="14" id="KW-0347">Helicase</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0408">Iron</keyword>
    <keyword evidence="17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication factor Dna2 N-terminal" evidence="24">
        <location>
            <begin position="408"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="25">
        <location>
            <begin position="1005"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="25">
        <location>
            <begin position="1106"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="26">
        <location>
            <begin position="1182"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="80"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="219"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="266"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1433"/>
            <end position="1463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="233"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="266"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="284"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="23">
        <location>
            <begin position="1444"/>
            <end position="1455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF08696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="CDF88828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1482" mass="167124" checksum="F04927B4F99C6017" modified="2022-05-25" version="1">MPTTPEKKRSGSMVASPEVGSSKSRPSATSAATERPGMERRRRKKNYQFAPINKLDPPVKTETNVLRSISVSQVRNTAFSSLKQSQKLKRASAPPQVVSRRENSLKPPARYTEEDGPTEEVIWKYSPNEKDLSDKVNSSNENSENLIDTDAVQDPSSTPMVPSRLKSVLSFCNINDKEQSEPQTTLSLHRRDCIRERGNPEFSKESLRDIDDILGDIEGDLRMKPNAPRLNDLPSSPSGITQHQEPQNIAEDSTIDGDESLIEFLTQNGSRGYTASKVSTTESPPRDRTGPDDQAHQSPLDNSSTESELLDDSLLEYFDDNTEENDENSLSMDRRDLEQVGMRLSQNLNIEKANSGTKQASPTSEFEEYIELAKCAVKREGIVRLVVLSATQFQLPKIGRQKILNCIDSEGKTSSVMVRHPWVYLEFTKGDVLHIIEGQNITNKRLLSDDVNPQTQLKNDNLLILNPDVLLSATSVGSSIECLRRAVLQTIIEDPRGEPSIVMTIGNIVHELLQEALRCKLEDEKLTTEYLEEVLDSILKTYSFAIFVCGATIEAVKDEVMKIHASNVLNFVNQFVRKNNFGCYVSVSGTRRTQPISIPSVLDIEENIWSHSFGLKGFLDATVLAHAGNEDAIVPLEVKTGKTRSISHEAQGLIYTLLLRDKYEVPVDYFLLLYTRNNDLTKYPKVLHSVKHVIMSRNQMAVRLKYKLSEIKRKEEFEMNLPPLLQNSLCDNCYAKEPCMVMNNLLENGTAKESGLRNGEYEALTRHLLLNHEKYKNFFKKYNDLITKEESSISHMNKQLFLLDSCSKESLSGLCLSNLCVVGVSGNVTAPGELLYEFKRSSSADRSQSMLHSQLNLNDKILISDERGHFALCQGYVNAISRDSIVVSTKKKLPDCRNIAHRISSATVEQPAGDTRRSMTYRLDKNSIEHGLSLTRFNLLNLFLPPIIEEHVPTDAHTDEPRQLKKSEGGDARMRAFLVDKVKPTFLDEKLSPLIPYNVSDISSFNSDQRTAIKKIARARDYALILGMPGSGKTTLIAQVIKMLVAAGKDVLLTSYTHSAVDNILLKLKPYDEKIVRLGSKGKIHPEIRQYMPCYEEAESYQDIIKQISDAHVVATTCLGLNNIFFTLKSKDFDYVILDEASQVSMPVALGPLRYGEKFIMVGDHFQLPPLVKNEQARLGGLEESLFKMLCEEQPHAVCELTYQYRMCEDIAALSNFLIYGNKLKCGSKQVAERSISLPRLESLKKFCSSRDSEQWLDYVLDPKKKVVFLNYDNCKQLFEKNERNHITNDSEIDIVEQCVEGLLHAGVDCGNMGVMTLYRAQLMLLQKRFHSDRHRTLEVLTADQFQGRDKEAIIISLVRSNKEQNGGALLKELRRINVAMTRAKSKLIIVGSRATISSVPQMQRLINFLHEKGWIYELQQSFKQCYTFPTEHSQEADTTTTRSQRKHKQKHKGGTHGAANIRSDSNVAKNHPIIRQALLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T5T7</accession>
    <name>A0A8J2T5T7_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S04-08570g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_08570g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T5T7"/>
    <dbReference type="OrthoDB" id="202825at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="entry name" value="Survival protein SurE-like phosphatase/nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027746">
        <property type="entry name" value="TTL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47551">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE PBY1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47551:SF1">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE PBY1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SurE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Survival protein SurE-like phosphatase/nucleotidase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF89617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="720" mass="81513" checksum="0AC691B6A51A1A3F" modified="2022-05-25" version="1">MRVLITNDDGPPNDEFSPYVGPFVRYLRQNYPEWQLTVCVPHVQRSWIGKAHFAGKSLSAQFLYSKIDAEDNLCLGPFVQPQVPLEGSKLPRNRQNPEVSGDDIEWILVDGTPASCANIGLYHLCEKPFDLVISGPNVGRNTSSAYISSSGTVGAAMEAVITGRTKAIALSWAYFNGLKHFDSKTMDLAAQKSMQVVTYLYHNWNDATDLYSVNVPLSNSLCSDTPAVFAPIWENRWDAIFEGPKVAREQSGGEIEDGVEGQMITFKWAPFKKSHTNSQYSAATATDRDVIEAKKTSVTPLRATFKEVEGLVGQLSLSPTKGTNYFVITVDNGDYLYGPLSRAVSKHLPQFRIVSELPCTPVTSIFHYGEYEHLNVDKLATDANDYFANSYIYRKALIRKHYLAHTIHAYIVKYPDSILSQAALETFPLELDYAEFLDDALDENWELRQELKKQDKWWIVKPGMSDKGQGIRVFKTINDLQAIFDSFDEDSTDDEGEFFDDNKVIISQLRHFIVQEYLADPLLLPSMGNKKFHIRCYISCRGALEVYVYDRMLALFAPSAFKPLDGEKFSPTELKDLECHLTNTCLQSKKHERRELSVAEFDALLDISPENKLKIKEQIHSIAREIFLAALKVNAVNFQPLPNAFETYGVDFLVDSHFNVKLLEINAYPDFKQTGEELKGLIEELFQHTVSQLIVPFFNNTTPQSSENFVKVLEYAPHNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T663</accession>
    <name>A0A8J2T663_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S04-10792g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_10792g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T663"/>
    <dbReference type="OrthoDB" id="5554140at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009976">
        <property type="entry name" value="Sec10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048627">
        <property type="entry name" value="Sec10_HB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100:SF1">
        <property type="entry name" value="RECYCLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100">
        <property type="entry name" value="SEC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07393">
        <property type="entry name" value="Sec10_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exocyst complex component Sec10-like alpha-helical bundle" evidence="1">
        <location>
            <begin position="157"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF89709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="778" mass="91458" checksum="FE74E45B48D382D1" modified="2022-05-25" version="1">MDDLLKVKSIVLEIASNLNAKDYLNFRSVNRLVYYHHLNGPADKAVWKKKLVAMGLKEEQGTEITKESNNIISLDDDCERYDSATIFDYIRTFTKKDARSTYIKFYRCFQPYCRKLYHSNFTNFFPEKYDEDPLVQVRIFNYISRYNKSNTNDMDQYDRIDQNMNILKEIFVNSVLQEMETNYAANNYETVGKFIQVLLQCHEETNAVEFFRSKSDPSTEMKAPETVFEEDGALNHETFENALNSIRDYLNGKIEMVDILFGDRYPVILQFIETFIQENVLDFFNQLFEVNDSRLRYMPTIYFLVIQKLCTELKNSKNAGPTFHQIVRDFLNMYLEPRIVEYLELQPREFTKQTSLKFQHYEEEVASREKETNEQIYNSLKDQSKKKNNMSDDKNDFLSAFTKMLRIPNSETQKKQEQLQLAYNLNLITNNLQNIKSLVSLDLSCEIIQDAQSRTEEMYKFHLVENVLEIVKSKCQEIFKSLIDQLGNHHIKPGFEKATDLLQKYNSNDVERVELKLEGFETKVEPLVQFTELINIGDIILQMTSIFYKNELIHNNIINKNRDFLNDVVQAKKNFETMLDDFVAEGLNIGINKLMDEVEFVFNTTQMPEDFNPMEELGQREIKPTQCAIKVVELLSNHCFLLTGATDKGTIDVYQQEIAERFFNEVVKHVKKSLISTDGAIFLICDLNYYYDFIAYKLRQKRVVPLFIALKNVGQLYIISGKDSKELGKMICDVNRFKGIFSQEEIYEFVERRSDWVRVRRDVEKVMYGLGLKDCVIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T693</accession>
    <name>A0A8J2T693_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glutamine--fructose-6-phosphate transaminase (isomerizing)</fullName>
            <ecNumber evidence="4">2.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Hexosephosphate aminotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_07602g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="4"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T693"/>
    <dbReference type="OrthoDB" id="15235at2759"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00528"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00714">
        <property type="entry name" value="GFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000001">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000002">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000052">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047084">
        <property type="entry name" value="GFAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="12">
        <location>
            <begin position="2"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="13">
        <location>
            <begin position="397"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="13">
        <location>
            <begin position="569"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="226"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF89032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="724" mass="80808" checksum="CEB8A515EE8962C5" modified="2022-05-25" version="1">MCGIFGYCNYLVERSRGDIIDTLVEGLERLEYRGYDSTGIAIDGDQPESTLLYKQIGKVAALKEEISHDAPRRDVSFVSHCGIAHTRWATHGEPKQVNCHPQKSDPSCEFTVVHNGIITNFRDLKTLLINKGYTFESDTDTECIAKLFKHLYDENLSNGHELDFHVLTKLVLLELEGSYGLLCRSSHYPEEVIATRKGSPLLIGVKSEKKLKVDFVDVEFPDDNAGKPDTPLVSRSGTPNGSARSQTTNGFSGLRRTRPYDSNNLLPITPNEFNLRHSQSRAFLSEDGKPNPVEFFVSSDAASVIKHTKKVLFLEDDDIAHIYDGELHIHRSRREVGASMTRSIQTLEMELAQIMKGPYTHFMQKEIFEQPDSTFNTMRGRIDFDSGTVMLGGLQAWLPAVRRARRLIMIACGTSYHSCLATRAIFEELSEIPVSVELASDFLDRKCPVFRDDVCCFVSQSGETADTMLALNYCLERGALTVGIVNSVGSSISRVTHCGVHINAGPEIGVASTKAYTSQYIALVMFALSLSDDRVSKMERRREIMNGLRNIPDQIREVLSLEPRIKELCQQDLEEHKSLLLLGRGYQFATALEGALKIKEISYMHSEGVLAGELKHGVLALVDEQLPIIAFATRDSLFPKVVSTIEQVTARKGHPVIICNKNDDVWAKKAETKQLVTLEVPQTVDCLQGLLNIIPLQLISYWLAVNRGIDVDFPRNLAKSVTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6B3</accession>
    <name>A0A8J2T6B3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_09670g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10020333at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14019">
        <property type="entry name" value="STKc_Cdc7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000637">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF23">
        <property type="entry name" value="CDC7 KINASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="28"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF89122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="498" mass="57614" checksum="8A3CA0B599AAA4CE" modified="2022-05-25" version="1">MSHEDVPREIKEEMEQLYEDVAGLRDDYILLDKIGEGTFSSVYKAEDVKGKVYQKYGTHFWTQGSKYVALKKIYVTSSPQRIYNELNLLYMLTGCTRVAPLCDATRVRDQVIAVLPYYPHEEFRNFYRDLPIKGIKMYLWEMLQALSFVHSKGIMHRDIKPTNFLYNPEIGRGVLVDFGLAEMQPDYSREDVEEDTLALIKDFRSHEQFCPCIVRDSAKQNTASPMVTIQNGKVVHLSNVNGVDLSKGYPKNETRRIKRANRAGTRGFRAPEVLMKCGSQTTKIDIWSVGVILLSLLSRRFPLFQSMDDTDSLLELCTIFGWKRIRKCAALHGLGFEVSGLKYINENCYQGGLKGFVYELLSKECTVGTFPDYSVAFETYEYLSQELHDRRSIEPRLPKVEGTSDGPQAYELKKYQEEIWSDHYWCFQVLEQCLEFEPQKRSSATELLRSAFFNELNEADVYTDVETTDEDESVEEAEQMHDDDVLLIXKKKKIKKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T6Q2</accession>
    <name>A0A8J2T6Q2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S04-10858g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_10858g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="192608at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040108">
        <property type="entry name" value="Laa1/Sip1/HEATR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046837">
        <property type="entry name" value="Laa1/Sip1/HEATR5-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057981">
        <property type="entry name" value="TPR_LAA1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21663:SF0">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21663">
        <property type="entry name" value="HYPOTHETICAL HEAT DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20210">
        <property type="entry name" value="Laa1_Sip1_HTR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25808">
        <property type="entry name" value="TPR_LAA1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LAA1-like C-terminal TPR repeats" evidence="1">
        <location>
            <begin position="1837"/>
            <end position="1991"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF89712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2009" mass="229166" checksum="922A07C6C2167D2E" modified="2022-05-25" version="1">MPVLDLVEFIASSNRKRHDLFSWVTDNFDILYRGQNQENTDDYTKKLLEVQGQLEKFVRYSANLEEEKNVNSHYIYIRVAQLYTFCLTQLEKGHNGKSYDAAELLAKVLAEKYIEFEVEEKIDGKEKKKKKSNSKKTYVYSPCKDMACTILTQFYETFGNGLSALGPLISESIFKNLKKMLEKTKYIHASFMTSLMQLQAAILKNTHNMPTSYTFYSKFSKLSKLILDSIQIDDEDYPVNFLSSIIEVWALYLRQDQFIKENSNDLMTNLCAKFSEREWGMYGMVNDETRSYTARSLAEVMFHFYRLRKLITLDQVWEAYAEIFGEDMPRELEVGCFESIVHFIGLCSASNNEFFGGTHYLQIVRSLSCALFERAHARKQGLESMSRRLRYFEHMHDIFLPRIWDSSKSQILFQIMGCTEKEDYHDSNVKADMRFSINANAELQWYTLMQLELVRRLLGSLSSSFGNEQHIVQHIKEELIKLSTCDVFIIRIHAVEILKIFLVSLPEYLSETIEGQLTALSNNFKHEENFPFSINHGRALIIANLIDCADKDYVSYELIMRITVFATAFIKNHTTSTVSSLYFKGLVCWILLIGLVNYKDEQYLAMQSSQLFLFWKVLLTHTFSYRDQDELYKNLEIRNHALVCLLAYLGNVSIEKDVAKQVSYLLTKCSNYNHSITLKSGSIDKALLINEYRLLQVYLKIHEFIKKDFNSSLLILIIKNFSDPNLYGESVSPVVDSLKKTGTKKPQLKDTGTENLALEASVNTLLCQNDGFAFGLSSKIYSSGVTGLSIKSNKAPDFQVEHSGNSRRYYWYEIYENEITKPILPILCLDYLIMLYGVGGYSGKDRYGPRITTSLIDSSMEIFSLVFPYLNSKIQYSVIESLNLSMFSKMTTPLRSVAIAANSIVAIHGALKIIQEDGLSLEISVGQLLMESVKKINFYDDPFLTYLKADCIGMITAAVSRGIIDKPSPEYVIEQSNILIKNVVDVTDPYRRVLHALSLSAIFKYNSHIAKFNSFFEVIFALLKDPHPVVHAWSLKAMHILLKKYQSIDITIASNLMLTLEEVATDPEFGVFGSSIIRYNHNKEFNSHIVIGQIFCTLTETIGPSILDLDKIAIESFKNVTFGCISANDAVCESLGLSIYENIATFKLNDILCDEVFIGLAKDVIAGSFITGFGSTYYNCHFTGSNKLIPFTTSLRATFNEFRLFTQLLRLQRGAMFVKSVDIQSWRYLTLYPNCEELIEYFFAWLEQTYKQDIYWFDKLCLMFNMTRSKLFQGFFQKMDIILQQEGLKKAEEKQIKGEEEESITKSSKKNVKNGGSEGSYNICWLAKMVILRLILFLCKKSQTDKDLFTIFGMQIPTLIKISFQASTTRVSSMKNLGLEILNILLKQYMRINETNEHLMIEQQEAQITSALMPAFSSGSSPDNVASAINVTAEFLGSKIAPLERMSRISHLLVALLGNFTDKTSNMKVGETKIITQRARRKVELAILNAWAWLVHSSIAKKNDELFVFTKGYLDAVVPLWIISLREYVMVKYEGVKTRGEVEDGKKESFIESRNTKLELYEPVWLNFVIALGSLMETDHEYISRCLNNEELESFMFILFAQCMETVVKNIDDHSTKMKVLPALHNILNNSLPINSLFEDDIHSEIVGILDRLMIMGDNSERIQLIYIINDLIVGYTKQNTTHEAFLEGIDKLYELLRLLLMPIAKILPFIKYTSSEGGSNTNFNLTESDLCLLKLAFGVFESNVSKFDDLFKVDLYACLLFIMGRIYESEAANTVVGTILPLLKSLTRGLIAHSDKVSMLDIFFSSIKNILHENVDEEKQLATILILLTNGYDGFDDADLESFSKLLVNGLCRSETQAIAVQGFKTIINLSYKYKNCSILLKKAINTMSSILRVENSVTVHKLALEIIGFFNKKVLDEQPDKSNFTISLVLVFVLNSCNTCPDIREAAGDKILAIMELNPAAFKSAISAGLDLEQRKTVLKIVEDSSQYKNLDRSTTSSVPLKLRFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T763</accession>
    <name>A0A8J2T763_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Ceramide very long chain fatty acid hydroxylase</fullName>
            <ecNumber evidence="18">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">BN860_07184g</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Ceramide hydroxylase involved in the hydroxylation of sphingolipid-associated very long chain fatty acids. Postulated to hydroxylate the very long chain fatty acid of dihydroceramides and phytoceramides at C-2.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18 19">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="18 19">Binds 2 Zn(2+) ions per subunit that likely form a catalytic dimetal center.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the sterol desaturase family. SCS7 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="18"/>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T763"/>
    <dbReference type="OrthoDB" id="2204368at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080132">
        <property type="term" value="F:fatty acid 2-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000002">
        <property type="entry name" value="Cytochrome b5 type B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014430">
        <property type="entry name" value="Scs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863:SF1">
        <property type="entry name" value="FATTY ACID 2-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863">
        <property type="entry name" value="FATTY ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005149">
        <property type="entry name" value="IPC-B_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 18" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="17 18" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="10 18" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 20" id="KW-0349">Heme</keyword>
    <keyword evidence="14 18 20" id="KW-0408">Iron</keyword>
    <keyword evidence="5 18" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="15 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 18 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11 19" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="307"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="22">
        <location>
            <begin position="10"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="20">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="20">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR005149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR005149-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CDF89558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="385" mass="45232" checksum="A74DFF9E9E764548" modified="2022-05-25" version="1">MNASVTSPTLQLFSKEQLSKHNNEKDCWVSVWNRKIYDVTPFLEEHPGGADYILEHAGRDITQVLKDRDIHEHSETAYEILRDNYHVGYLATEEEEKKLLTNDAHKVEVHMTNSETVFDSTTFVKDLPTEDKLSIATNYSNDYNKHKFLDLNRPLVPQVMFGNFSKDFYLDQVHRPRHYGKGSAPLFGNFLEPLSKTPWWLIPCVWLPIVSYHIYVGYTHMNKLFSVILFGVGIFVWTLIEYGLHRFLFHLDEWLPNNNAALTMHFLLHGVHHYLPMDRFRLVMPPTLGFILCAPFYKTVFALLPRYWAYSGFAGGLFGYICYDLTHYFLHHVKLPSFMRKLKKYHLEHHYKNYQLGFGVTSWYWDTVFGTYLGPDAPLSHMKYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T773</accession>
    <name>A0A8J2T773_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 15">DNA replication licensing factor MCM4</fullName>
            <ecNumber evidence="3 15">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN860_02762g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3 15"/>
    <dbReference type="EMBL" id="HG316459">
        <property type="protein sequence ID" value="CDF90190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T773"/>
    <dbReference type="OrthoDB" id="10251574at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097373">
        <property type="term" value="C:MCM core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17755">
        <property type="entry name" value="MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000011">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000217">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008047">
        <property type="entry name" value="MCM_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF66">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21128">
        <property type="entry name" value="WHD_MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01660">
        <property type="entry name" value="MCMPROTEIN4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="17">
        <location>
            <begin position="507"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="77"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CDF90190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="922" mass="103071" checksum="133C79961E404FC3" modified="2022-05-25" version="1">MSDQPSSPALHPSSSSPDQSSDAGPIQPSSPAAQPSSPILFYHTSSSAQDTFGHDDSPNQSGRVNAALGSSPFKYPSSQQQSSDNTQSQSRDEPRITSTYSNRSDRLAPLSTSSQGTRPGHRAMMRRNDIHASDLSSPRRFVEFDNPSHISSSSSSALPSEAGEPLRIIWGTNVSIQECANNFRNFLMSFQYKYRKILDGKEQSINSTTDEDSYYVKQLNEMRELGTSNLNLDARNLLAYKPTEELYYQLLNYPQEVISIMDQTIKDCMVSLVVDNNIEYDLDDIETKLYKIRPYNVETHRGMRELNPNDIDKLISLKGLVLRCTPVIPDMKVAFFKCNVCDHTMAVEIDRGVIQEPARCERVDCNEPNSMSLIHNRCSFADKQVIKLQETPDLVPAGQTPHSVSLCVYDELVDSCRAGDRVEVTGTFRSTPMRVNPRQRVLKSLYKTYVDVVHVKKVSDKRLGVDTSTVEQELLQNKINHSEVEETRKITDQDMSKIHEVAKRPDLYDVLARSLAPSIFELDDVKKGILLQLFGGANKKFAKGGRYRGDINILLCGDPSTSKSQLLQYVHKIAPRGVYTSGKGSSAVGLTAYITRDVDTKQLVLESGALVLSDGGVCCIDEFDKMSDSTRSVLHEVMEQQTISIAKAGIITTLNARASILASANPIGSRYNPNLPVTENIDLPPPLLSRFDLVYLVLDKVDANTDRELAKHLTSLYLEDIPENASTDDVLPVEFLTMYINYAKDNIHPVITKAAKTELVRAYVGMRKIGDDSRSDEKRITATTRQLESMIRLSEAHAKMRLSKLVEVTDVQEAVRLIKTAIKDYATDPKTGKIDMQLVQTGKSIIQRKLQEDLAREILRVLADYTTDSMSFNELSRQINEHSQDRIDASDISEALSRLQQEDKLIVLGEGIRKSIRLNNRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7C1</accession>
    <name>A0A8J2T7C1_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ZYBA0S04-04522g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN860_04522g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7C1"/>
    <dbReference type="OrthoDB" id="4069217at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054776">
        <property type="entry name" value="VIR1_yeast"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22575">
        <property type="entry name" value="Vir1p"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="624"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="624"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="642"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDF89447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="803" mass="89553" checksum="79366F2014AF4823" modified="2022-05-25" version="1">MSKLNESYPLVSNETAEALVSSTVNALKCLNSNGDNQLIFKVIDALGLFSHKVVAGNVKQDDLEGIDVFTTILKSDEIGNKLDVVAIFLAYFSIHKELHPLIINSIESWNTLLPHEKATEASDTGYTPGFYTVVSQLDQYEEVTPQLLQYLDLELGFVLTQVWVPQWLQLVRAPLETSARQLQELPLANMLLCEAKLDFYIATSTSLGPLVKGKQRQEVSPMSYFIHKICKRISIFPDWIPKELYRPAAKIASYHGSSASGLHFDLQVLMEITDHPDINFFEEPRLAILLHQATNNLRKVPFHKVHAALGTLGTVQSLPAIINIVQFLLCKFLITVGNVCDLIKKADYQLELHPKEKKWFKAAHNDTYHIPCWFESTLLPPLPPITKSFFVFENNELESEQEANNFSMISDLLYESLDMVILINTELLKQYQCMKIDPLTIDAADETFDIRHRVASQYLLLYFIPLITASMLSRQAAGSQVGLLNNKKRHIMGKIIFSNTVRLCKSVIFAHANIALYHFIKLCAKVSTEDLILQGICLDLLYHLFFGQDGAYIRQLCLESELSIQTLQSYINLWNDGTDCYTSFFEKILEMKQPSVPIKSLPMSKLHELLPGCDAICSTQGKNATPKSLSLTPKNDPQQKGSLSSRSSNIGSSPPLTYKYNPYSSSSFAPQRPLTFNTPLPSGTYVSSCSAGSPETGTFKNRIGDGTTANAANGSNYNVFDNSLASESFSNLSRTIENLTGPPRTPSTSIFSSPWDGSPSMNAIPSNGKIVNTGKNYILGGHHRIKNNSRAQSIHIDNFKSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7N3</accession>
    <name>A0A8J2T7N3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN860_16468g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7N3"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF15">
        <property type="entry name" value="TRANSPORTER SEO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="499"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="90"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CDF88702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="534" mass="60628" checksum="2399300F65E6FAB9" modified="2022-05-25" version="1">MWKKDFEGTEVAVLDSEIADDDGNKEETITNSEKISNSTWRKLVPCDIELTEEDYAFLNTKVVSQPWYKYFSPTDTEEERRLVLKLDALILVYLFLSSFVKTLDSSAVTYAYVSGMKEDLKMYGNQLTYQSSCFMAGFIVGQIPLTLLATKFPIHLYLPLMDTVWALFTLFIYKIDNYHQLYALRFCTGLFGSFFFPTVQYILGCWYKKTEITLRAAMYFTASQVGSMATGYIQAAAYQHLSGKAWLDGWQWLYVLAFIITIPISLYGLFTLPGLPESLRPQSSTKCWPIRIFVTYNLLTSGERKLARLRMIREGRISNEKFKFSTILKCLKSCRFWILVIFAIFFSQADGISSNNGLPLWLQAKNYSVYDINTLTTVIPAVTIAFSLLNGILVDSWGKDSWSYPITIAYVSILNLIAGIILAVGNVNNGATLFAFFLSGTADSIAAVIYSWANIICSNSPQERALTLSTMNTLGNTFGVWVPLFVWKTEDAPRYFKGYLYNVALDAMMLLLLPVVVWLHHKQLRTGKITIVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7N6</accession>
    <name>A0A8J2T7N6_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Signal recognition particle 54 kDa protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_09934g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Signal-recognition-particle (SRP) assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum (ER) membrane. SRP is required for the cotranslational protein translocation for ER import and preferentially recognizes strongly hydrophobic signal sequences. It is involved in targeting the nascent chain-ribosome (RNC) complex to the ER and is proposed to participate in the arrest of nascent chain elongation during membrane targeting. SRP54 binds to the signal sequence of presecretory protein when they emerge from the ribosomes. SRP54 interacts with the scR1 RNA and mediates the association of the resulting SRP-RNC complex with the signal recognition particle receptor (SR) via its alpha subunit SRP101. Both, SRP54 and SRP101, are locked in their GTP bound forms in the SRP-RNC-SR complex, which dissociates upon transferring the signal sequence to the protein-conducting channel (translocon). After signal sequence transfer, SRP54 and SRP101 act as reciprocal GTPase-activating proteins (GAPs), thereby resolving their association.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Fungal signal recognition particle consists of a 7S RNA molecule (scR1) and at least six protein subunits: srp72, srp68, srp54, sec65, srp21 and srp14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The M domain binds the 7SL RNA and the signal sequence of presecretory proteins.</text>
    </comment>
    <comment type="domain">
        <text evidence="13">The NG domain, also named G domain, is a special guanosine triphosphatase (GTPase) domain, which binds GTP and forms a guanosine 5'-triphosphate (GTP)-dependent complex with a homologous NG domain in the SRP receptor subunit srp101. The two NG domains undergo cooperative rearrangements upon their assembly, which culminate in the reciprocal activation of the GTPase activity of one another. SRP receptor compaction upon binding with cargo-loaded SRP and GTPase rearrangement drive SRP-mediated cotranslational protein translocation into the ER.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7N6"/>
    <dbReference type="OrthoDB" id="10250817at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17875">
        <property type="entry name" value="SRP54_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000022">
        <property type="entry name" value="Signal recognition particle 54 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="entry name" value="Signal recognition particle, SRP54 subunit, M-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006325">
        <property type="entry name" value="SRP54_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01425">
        <property type="entry name" value="SRP54_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF5">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="Signal peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10 13" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="14">
        <location>
            <begin position="275"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF89674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="529" mass="57934" checksum="B3F05AF1333180FD" modified="2022-05-25" version="1">MVLADLGKRINTAVNSALSNTQDDYATTVDVMLKTIVTALLESDVNIKLVSKLRTNLRTKLLDAKTGSKSTSNAQTKKLIQKTVFDELCDLVDCKAEQSFQPKKKKTNIIMFVGLQGSGKTTSCTKLAVYYSRRGFKVGLVCADTFRAGAFDQLKQNAIKAHIPFYGSYVETDPVKVAAEGIARFKKEKFEVIIVDTSGRHHQEQALFQEMIEISAVVKPNQTIMVLDASIGQAAEQQSKAFKDSADFGAILLTKMDGHAKGGGAISAVAATNTPIIFIGTGEHIHDLEKFSPKSFISKLLGIGDIESLLEQFQTVSNTEDTKATMENIQQGKFTLLDFKKQMQTIMKMGPLSNIAQMIPGMGNMMSQIGEEETSQKMKKMVYVLDSMTKEELESDSRIFIDQPSRLVRVARGSGTSVFEVEMILMQQQMMARMAQSTKAAQGGVGAGGMPRIPGMPNMPGMPKMTPQMMQQAQQRLRANPGLMSNMQNMLGGRGGFPGAGGMPDMNEMMKMMQDPQMQQMAKQFGMGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T7V4</accession>
    <name>A0A8J2T7V4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S07-04060g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_04060g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatase 2A regulatory subunit A family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T7V4"/>
    <dbReference type="OrthoDB" id="340346at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000062">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit A alpha isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054573">
        <property type="entry name" value="PP2A/SF3B1-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051023">
        <property type="entry name" value="PP2A_Regulatory_Subunit_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648:SF4">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A 65 KDA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22646">
        <property type="entry name" value="PPP2R1A-like_HEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="52"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="91"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="169"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat" evidence="4">
        <location>
            <begin position="291"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="296"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="336"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat" evidence="4">
        <location>
            <begin position="372"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="375"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="414"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="533"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="577"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF90567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="610" mass="69118" checksum="0E1C4ABDB9613FCE" modified="2022-05-25" version="1">MVSEPADDKNLYPLALLMDELKHDDIANRVEAMKKLDTIAVALGPQRTREELIPFLVEVAHDDEDEVFAVLSEELGKFVPYVGGPEYATILLPPLNILASTEETLVREKAVDSLNKIAEVLSQEQLFHDFIPLIQHLADADWFSSKVSACGLFKSVIVRVKDNGMRKELLALYLQLVQDDTPMVRRTAARNLPTLIDLLTQNLNLSTNEDWDYISSMFQKIINDNQDSVKFLTVNSLISILKFFNAKKDFSHTQDLLESAKKLIGDDAWRVRYMAADRFDDLAAQFEGHDVYIKELVQPFLSLCEDNESDVRKAIAKQVPGFAALLKDSSIVSTKILPAVQSLSMDESEIVRASLALTVTNLVTLMSREEAIDHLLPILLNMLKDEFPEVRLNIIANLKVVNDVIGVDLLSESLLPAITELAKDVNWRVRMAIIEYIPILAEQLGVQFFDQQLSDLCLSWLWDTVYSIREAAVNNLRRLTEIFGSDWCRQEIISKLLKFDSQLLENFVYRFTLLAALTALVSVVSNQVVAEEILPFVSRLADDAVPNIRFNIAKSYAEIVEKLSHDRTYSGLIKHTILPSLDKLRQDEDFDVKYFANQSLEKCNMILKHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8B8</accession>
    <name>A0A8J2T8B8_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_09824g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T8B8"/>
    <dbReference type="OrthoDB" id="8693905at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF26">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DDB_G0283821-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="35"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="350"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF89128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="948" mass="107768" checksum="B8880CF8D205FC50" modified="2022-05-25" version="1">MTERINLTPAQQQQTVSQTERSSKLEAQKKSQIQYQLKQVIGKGSYGIVYKAMNRKTQKMVAIKEVNYDNDDELADIMSEIDLLKNLNHINIVKYHGFIQKSHNLYIILEYCSHGSLKNFISRGNGLPEAKAKVYVKQTLNGLNYLHEQGVIHRDIKAANILLDSNNVVKLADFGVSTKVNTMTVAMTVAGSLNWMAPEIIGNRGASTLSDIWSLGATVLEVLTGNPPFHNLVDINIYYAIENDAFIPPSFLSKEAQDFLSICFQKNMYQRPTAAQLLKHRWVAGDSPANKLELYKESNNDLDFQWDQDFGDSLQETNLRRPSLQHSSVSTSCLPSLAKTRVTYSSASSASSTSLVTPGTSAAATRSSRDPLSSTPRRPVHKSGVKEEEAGILLNSLGKETVQVSEEELSFLFGYSNMEDIVQSVIQLLRESSNENLVLSIFDYDKKYNFGNLRKKFVMMGGVPSILEYNSIIEQFFIKDSYKVLIQCGILYRTHILKDSIILELTYKFLDMLDSPQFWCHWCSKHLKIPNLIANLGNRSAQSIVLKLSSYHEASNWFPPLFLECILKLPPRANQPLHIIFKALASILIQSHNNATAQIVASPSSKVSMALTSTMDFPDTFIPWVLQFLEDPKFYEMENAYIWKYYMNVIFQATHLHRRLLNQLLGNDRFISLIRKLLGNRENTTQLLYVPLSIVMEISTEMTKFYPVLVELGLNFIHAREEFIVGGLEILLNSLQFALHDPTKFVRDAEHMSIRFPSGSSSKPPVFLSYQDLVDPFFKIRENNLEFGNFIAKFIKICFLTPLDFISFKILVHPDFNERVTAFFRLYRNSLLIQIDLLKFVKLVFARSLEYLNMTKEREADAENIVISKLEKMTNFLDRNWDHQIEGQVGCDSILIQQLCNDIKAFATIPPTTFTRSSIHIQRSRGQINSSLENDRSALSALRSGVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T8W2</accession>
    <name>A0A8J2T8W2_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S10-01046g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_01046g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316463">
        <property type="protein sequence ID" value="CDF91160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6287725at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025614">
        <property type="entry name" value="Cell_morpho_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025481">
        <property type="entry name" value="Cell_Morphogen_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039867">
        <property type="entry name" value="Furry/Tao3/Mor2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029473">
        <property type="entry name" value="MOR2-PAG1_mid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295">
        <property type="entry name" value="FURRY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295:SF30">
        <property type="entry name" value="PROTEIN FURRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14225">
        <property type="entry name" value="MOR2-PAG1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14228">
        <property type="entry name" value="MOR2-PAG1_mid"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14222">
        <property type="entry name" value="MOR2-PAG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cell morphogenesis protein N-terminal" evidence="2">
        <location>
            <begin position="314"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1373"/>
            <end position="1596"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1665"/>
            <end position="1835"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis protein C-terminal" evidence="3">
        <location>
            <begin position="1869"/>
            <end position="2119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2248"/>
            <end position="2272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2335"/>
            <end position="2366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2414"/>
            <end position="2464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2346"/>
            <end position="2361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2427"/>
            <end position="2437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF91160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2464" mass="277518" checksum="FF20AA2ED02B67A3" modified="2022-05-25" version="1">MNTGFTFPPQAPPTGGDGFRFPNSTPSAEMTQAAPSNSFVNQGPPFPEIADQESDLAPIIDIPPPPEISDKIVRSFTPDTKKVPSTEEESTTQPISQHLIVHSVVPPSAEQLVTSKSPIDPQSKSQEEEYVTNLRQQMATDWKSPSEYALHILFTKFVRHAENKLNECLHHSSEPSEPPIVDILGEGIDPTFDKIIESLGHIAKNKPKPVIDAMMFWRKTKSEIAGVAAEEVECLLREYESDQGKPTPSKHQHSSSISQTKTHAKSVSNGSRFSHKRNSSSKSSTGSRNAIENQRIKNLELGIISSREAALQADRKSLISIYILCRVLIEIVKQAPDGGDEDLNDKLEEIVFTQLKTTDPMSISSSIIKSSNWNSFAELLGWMSEKKFLSVSDRFIADLEKIPSHVTRDLEPGVHLLILGMRYLRLKNYPLEKFEEAADFMKSIAKFFYKAENLSVSLAYAEVISQLLLPLAGSMTAEVNHPTWVEAMTALLTTAAKLQTESSKYWASIFRLNVSVLCVSPASLFSEHWIPLIEKNARKIKNKNLSSRVLFVVGISRLVWVYLYRCPETLNNTTRTLSRLFSLFLSTKKKENWITTDLELINPLSDVFVTVGYAHPNFLMENAILPLIRNSFNGFSLDGINYERMILVINTYRGLLMTNNKPEFPENDSRYYELSLNDISIKQNEALTSNHEEVCTHFYKLFLLLDSNIGSEVWSPENQHNRQPTTAFEKFSIFSNDNGNNNNRNLNIALFATLIETIPSCLTISGQIPFKSTIEIISRNAVHADLLIATSCQNALKVLASKKNPYTLITWFGKYSFDFDEKTQSSYNMSYLSSTEYRRLLILYVELLECWLDEFHSSNMEEKKRETGLDGIELMPVDNLEEMNEVETLGWKNTITVIEEVEGNGLFFLCSQDPGIRRLGIQILGIISKFDEAMVEKTLKISRGHSRSSSYFAADRGTRLVDLLNDLDFYSLLDNKKNLLSVVEKSRISKLTAKYKKGVLIKLGESEYGVDAALWQRVFPKLLSIVCVSCPITMALCRSIVCIRLVQVHGIILRIASTMEDKSRELLPEVIVNQWKLFLIVACTSLTSTNDQKLHIPISGLQHGRKKSQQIFTVQHQKIKSATSVFKMVLPLLNAENALIRDAIISGLSSMNVNIYKAYIESIEGFLSTWSLQSSNNKIRIEMFHVLTILSRFLSEPMVLEDKVLLKKISEILKYAKKFLDEESVQYSYEYQTLRGYFAELLRSYYSAVRGLSNFDELFPFQARASCFNFLKEWCGYGEHAYIAEERYNTMIRSTANSRDRTAITAGIEFQKGRLQKIALETMVVLCSDTITKTLNDVPEMPIIVSFDITGLLSWIEALFNAEDESVKNLGISSLENLLEKNTRNRTLFREVAIQCAAHHKHPHVSVLYYITLCKTVLKMDDLILEEVELVSLGLYGIISDREDTRSYAVDLLSAVETKLHNSFYTKVFKERLASSSKTVYKSTAKEISSIFAELLSQELCLKIFSTLVGMMDSFTFQIKKDLLILMAPWVNKFTLKSIEELETFMVLSNLLYITIDLNNKLPMEVEQLWISLGKGNTFQNIHVSLEFIMSLSINHRNPHFVQHARDVVLYLGNVPGGIGLVDSLTSNLEPKTMIPIAKYDTAEVPENDKSYFIANIWEKLSYHGKSITFSKAHLSIIFLVNLLTNPSETLKGNVANLLHVSICLLDHYVPLIQESASKILCDLVFGFAPTHEKSEDTVELLRNKQNIWSYDSFMKDKNAVRSPKAMDLLIRNLLSIFSSISGLQIDWQKIALKWATTCSVRHITCRSFQVFRSLLTFLDQGMLRDMLHRLSNTIADANPDIQGFAMQILMTLNAITAELDPADLINFPQLFWSITACLNSIHEQEFIEVLSCFVKFISKIDLDSPDTVQCLIATFPSNWEGRFDGLQQIVMTGLRSANSADITWKFLDKLNLLKDSRIIANSDSRLLFALISNLPRFLNAMDRKDFTNIQTSVEAMVNLSESKGQPSFSRLLNSLAKNKFRSKKDFLSQVVSFISRNYFPQYSAQTLVFLSGLLLNKTGWVKVQAMEILKYIFPLVDLKRPEFTGVGADLLSPLLRLLLTEYEANALEVLDCVPAVSGSKMDKDVLRISMGNKDVKNTNKVTTTLFGIPEDSGWSVPMPTMTAATTRHNVHAVFMTCATSASGEDSTEDMPTMDDIVEFHADADYGLGRTETNDSQSMLEEKEASLSHMWAELDNLDSFFTKNNTASVSATPRDGAGPLHDRSNSADTTQTEQTFALESAPQLYDKKVSAILNHSLTKIPSNVSFKTYLADSFATSMGNDGITNELGPRSAHAIYGEESGAPPRSRNSGSFRSPANSLSLSGKARVAESPQDSLFRFEGFLRSSQRGSRRKWQQHQQHQQLLSQTIHEQIVPSYYESEPTSPKPPVRASSASPSASTRGIGRSQKSQKHYHLPHFSSKSTETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T967</accession>
    <name>A0A8J2T967_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZYBA0S04-10198g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_10198g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T967"/>
    <dbReference type="OrthoDB" id="430364at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF405">
        <property type="entry name" value="ARF GUANINE NUCLEOTIDE EXCHANGE FACTOR SYT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="2">
        <location>
            <begin position="369"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1034"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="307"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1038"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1065"/>
            <end position="1080"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF89686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1126" mass="126001" checksum="23E0A8BF4A87293D" modified="2022-05-25" version="1">MSQTLAHLIKNKIFQGASPTGDEKKKRGRRWGHRKGDSAEDSLQVSPSKQAEAGLQFPMSPLNSIGEESRSIGSESESELEGISEFEEEGIIETDGVPCEEEDTASDSPVGSSDSERRQGKLHLLKSKMSIPDLSFKPHKLHNHHARQKSIERSPTLSKANSPHSVPMSPGSIARDLPLQRPPNERSASGLGSIYKLGSNSSTSSSRFFYSPRRSANNISNLTSSNNGTQISVVSKTNSGINLFGTNSSTQLMRGHIRNSSVEMKSVKPQPLSIGRRRSKTLDTYGDYKKGWDTTGDLLSTINAHLSSSRSNSIKSRSPSLKTQESISGSATTVAGETPLDPSSPGPAPPRFSNGGGSIPFLSAPVQPQATTSSRRGSSIANAFNSFVNLRSFSNTSSKGTPTTNASRVEMTEGDLSHAPEPEAEEDYDEYLAKLAPYGKGIATILCEEDDAFKRACLSHFLMNHFDFSQCPLDIALRIMLIFLQLPKEAQQIDRLLIEFGKIYYKVQSEKEVGCLWVDESQVYFVTFSLLMLHTDYFNPKNRFKMTKQEFISLIHEDKESHGCKVPREILSYFYDNITAKEFTNFDFVPHGVNSSSCASPEPDRELGGGKVIKFYSPVDIIRTQASPSSSDFVPLATTQTGRASSNSFSSYLPHVPVSTSSSSLSLVQDDVDVYSHIFDDSLSSLSLTPVVQKVWDEDYLYELFANQNKYNKYFSILKGAKGGYLRLSRQDLHKLAFQGYEVLNMCESDLDNLHLKIIQMGEIDELTLNRKFTIVGAANKTMWKRQLGILTTCGLLMFDNLNWVNPRLVKDESTGTSNYIIDYHSSASMTVESPIQVRGLLAVNKTHKLLKQALDRVEAIEEENESRTSSLTNVAHADDQFKEPNGDCIMRLYGSQKAKVWRCSSQYEKENWIDAINLMAAHDGCYIHSDAIKNTVVFSRKHSIKQRINKLKSTGLEKQRKLEESEKLLDVYAQMMPICIRTKNELSFHIKQLAVTMEWLVYEIKRSEINLKIIEQVDTQYCEQRDHDECDQEEDNRDACTHHKQGEQANEKEKGKGKGKGKGKGKEREQRRQNHHSDEPEGEDSMTTDQPFIFDEQLMHRNFAEEQSIIYDQSEVNSGGILASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9I4</accession>
    <name>A0A8J2T9I4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ZYBA0S08-02410g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_02410g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316461">
        <property type="protein sequence ID" value="CDF90761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2T9I4"/>
    <dbReference type="OrthoDB" id="3163292at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription activator activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="C6 transcription factor (Leu3)"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="prtT"/>
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        <property type="entry name" value="XnlR_reg_dom"/>
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    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRANSCRIPTION FACTOR SEF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="80"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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            <begin position="33"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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    <feature type="region of interest" description="Disordered" evidence="8">
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    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1029"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="49"/>
            <end position="67"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
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            <begin position="70"/>
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        <location>
            <begin position="246"/>
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        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
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        <location>
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    </feature>
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            <begin position="565"/>
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            <begin position="1034"/>
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        <location>
            <begin position="1067"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF90761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1157" mass="129082" checksum="F54353188CB21A95" modified="2022-05-25" version="1">MNDSPNVKGEERHLEFEIPLKRSLSHDHAVTLPEGSKKLQRCKSEDVHVSSSNTSPSVSHHNSSPHHAAGTSSGHRPVTSCTHCRQHKIKCNASDNFPAPCSRCERMGLNCQIDPQFRPKKGSQLQNLRRDVDELKLKIEYLTRNESLIARALQQSSMGQELINTLKAIDVSKVNELANGQPPQRLQQRSLRPHPQASKISVQTFLGASEPQLLQEKVKEETEDNNKNFYKKDMPLVLQMALQRNNSNNVNGGNNINHHSGDNTPNDTPGSDRASLSLSPMPSTYSGPKRAETGSIRDREKASPHDIENPGKNHKRSPVVATTNAIPLLPSPYAGIDEFVIGDVRISIDKANELHHIFVTRFLPYFPIMYTNSVTELYSQSQLLFWTVMLTACLSDPEPSMYIKLAALIKQLAIETCWIRTPRSTHITQALLILCIWPLPNQKVLDDCSYRLVGLAKCLSYQLGLHRGEFISEFTRTQTSMPDAEKWRTRTWLGIFFAELFWASTLGLPPTSQTDYLVEDARMGLDEEHESPSNNLDTTVNSETNGSHKEDFNEANQRPVSSEHSTPKNRDHREFDQNSDKKSKLPKNFRRLICLAHFQAKLCKVMGSSVVSPDALVEPRDRASSLAILERELESLDRSLIFSQDTAVHIYYLYVRLMVCCFAFLPGTPTEDQSQYVTESYLCATKVVTLLTSLLEKQQLVELPIYVRQSATFAALILFKLQLTPLLMKKFLDSARQSIVTVHRLFRNQLSAWATTVENDISRTASVLEKLNFVLITHPTVFVEEDGIISRMRSHLTGSLFYDLVWCVHEARRREMDPEYNKKAVEQAAAKRKARGEDSPFAASDRRLYPLPFYNQISKEDFETIIQTTPGGTTVSTLVPTKTALNNAKTLAKGNKYSNGTIMEINGIPLSMLDETGSVKLENILGTSNSINAPANAGNDSNTSMRPMVGASSNFTDPIAHSLNLGTTFGQKNAGKDAAAKEGVLQSMAPFMNEADLQRPNASFQRAHVAASADKRNRGSLFGVNNSTVSSNTLNKSFMPQQADGSTSSRVATKTASNNDDNDDDNTNNNNNTNGNNNNDNNRVNFDNYANLNMFLNSGQALAANSPADQNGNRKNAINQLSELDTFFQQQSTGWLEGNSSNDDFLGWFDMDMEPKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2T9R1</accession>
    <name>A0A8J2T9R1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Vacuolar protein sorting-associated protein 27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_04588g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the ESCRT-0 complex composed of HSE1 and VPS27.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endosome membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
            <orientation evidence="1 10">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the VPS27 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316461">
        <property type="protein sequence ID" value="CDF90849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="957735at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033565">
        <property type="term" value="C:ESCRT-0 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15760">
        <property type="entry name" value="FYVE_scVPS27p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21385">
        <property type="entry name" value="GAT_Vps27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16979">
        <property type="entry name" value="VHS_Vps27"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017073">
        <property type="entry name" value="HGS/VPS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049425">
        <property type="entry name" value="Vps27_GAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47794">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47794:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21356">
        <property type="entry name" value="Vps27_GAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036956">
        <property type="entry name" value="Hrs_Vps27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0967">Endosome</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VHS" evidence="14">
        <location>
            <begin position="18"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="13">
        <location>
            <begin position="170"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="221"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="311"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="528"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="227"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="248"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="319"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="330"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="553"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="581"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR036956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF90849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="606" mass="69134" checksum="F9395CF39884CE33" modified="2022-05-25" version="1">MSATSIGELESLIEKATSESIPHGELDLPVALEVSDVVRSRRLAPKECMRCLKKRAMNTGDNPNAQLSSWRLIDICIKNGGLPFIKEICSREFMDTMEHAILKNADNPEVESLLTRILYELYCAFRNDLQLSYVKRVYDKLLARGVEFPRELQDGSSPIAMFDSRTPADWVDSDACMICSKKFSFLTRRHHCRSCGGXXXXXXYEPVRVCDNCYEDYEARKHSTGGSKKKHHNKKKKSKSPTYDEDEELRKAIELSLRESRGSNEPIVPVMASRNPTPPSAIIKEEEALEEEDDPDLKAAIAASLVEAEEEKRRREMEAQQQQLNVQQHQWEEQQRQRQEPQQPSFDLTSTEEEDIHLFASLVERMKNQSATEILEDTQLQRLYQKVLGSRPKINQALNDSVQKYNSLIDMNTKISDIMNIYDDLLERQLQSINLSERYTVPQGPSDPYSYYEEVSYRQPQTGQNVASSAKQADSARQHHLDEIRDIQMSAETAHTARVDTSNILAETPYPTTEEEMESQASIQGYVPSQKTLQEPRGHGETPYPVEEEESRTTVASKGQITNFDFPTVPTRKVLQENSEGESHEVESIGKTEQTLPKEEELLIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAB5</accession>
    <name>A0A8J2TAB5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S14-00958g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_00958g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316467">
        <property type="protein sequence ID" value="CDF91781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2TAB5"/>
    <dbReference type="OrthoDB" id="1748564at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070319">
        <property type="term" value="C:Golgi to plasma membrane transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21044">
        <property type="entry name" value="Rab11BD_RAB3IP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040351">
        <property type="entry name" value="RAB3IL/RAB3IP/Sec2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009449">
        <property type="entry name" value="Sec2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430">
        <property type="entry name" value="RABIN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430:SF0">
        <property type="entry name" value="SEC2P DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25555">
        <property type="entry name" value="RAB3A-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06428">
        <property type="entry name" value="Sec2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144284">
        <property type="entry name" value="Sec2 N-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GDP/GTP exchange factor Sec2 N-terminal" evidence="4">
        <location>
            <begin position="31"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="537"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="664"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="24"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="543"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="610"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="620"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="664"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="681"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="734"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF91781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="743" mass="82353" checksum="3D5BD0A1ED24C96C" modified="2022-05-25" version="1">MAEQLDVEESKRVSTQIMTLSTQLIESIEKQSTLEKELNQANRAMESQTAAFEEHQQITKRLELLEKVVAEKNEGLKSLKEELRKEREAKAAAEANVDKLNREVEDLTASLFDEANNMVADARKASHALEIKNAKLVEQLQERDTLLETLNLQLRHLKKVIQNLDDNSAASEQVKALANASDSASLTASLSKTFSPNFGHHTELPLGPIFSPNITSVRYDLGLYNEFLKFIALLPTCQDIKDTSSESKLLRRLINDEIQPILKLENASGLGWIVKRTLVNLMMEGLVVVEPISGLNEKHQYGYGSPSLNQQNKNDLKDTSSEVHSITAKNIHLFNYPSDSPPVAVREKCAICAESRDDLVEHARLHLLKTQTRADDGTLTVTNTFPLCKYCVLKVRQTCEIFAFLRSLKLGAWKLEKVSLTGIDKGESREFSEVSSLSNNEDALKNVKDNKKESWNAHRMSFISSMGSLNKITTVTPEVPVSQSGAPTTNIQRAWVQLCRLRSMLHWTHIGIWNVEDSISSRIGPFAGDESDDELVKLDTRQSKSSSDQPSVIKQTLADQTPVLEEGSPSFKDRQNSEGDTFDFETNAEGQTVVTLLPGERGSVNDEENSSSIGRSNKSAPDEEKDINLDSNIPKDIDIPKLAETSSKDLMANLDRLGEQLKVESGKQDVSKDEMVKAKESNGTGTMTSEAASASLKGDSGSMSPRNDCIKSGKHRSIKSFIHSDTESASNTDDQFDDAQESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TAF1</accession>
    <name>A0A8J2TAF1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Fatty acid synthase subunit beta</fullName>
            <ecNumber evidence="21">2.3.1.86</ecNumber>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="21">3-hydroxyacyl-[acyl-carrier-protein] dehydratase</fullName>
                <ecNumber evidence="21">4.2.1.59</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="21">Enoyl-[acyl-carrier-protein] reductase [NADH]</fullName>
                <ecNumber evidence="21">1.3.1.9</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="21">[Acyl-carrier-protein] acetyltransferase</fullName>
                <ecNumber evidence="21">2.3.1.38</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="21">[Acyl-carrier-protein] malonyltransferase</fullName>
                <ecNumber evidence="21">2.3.1.39</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="21">S-acyl fatty acid synthase thioesterase</fullName>
                <ecNumber evidence="21">3.1.2.14</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="24" type="ORF">BN860_02278g</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20 21">Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The beta subunit contains domains for: [acyl-carrier-protein] acetyltransferase and malonyltransferase, S-acyl fatty acid synthase thioesterase, enoyl-[acyl-carrier-protein] reductase, and 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 21">
            <text>(9Z)-octadecenoyl-[ACP] + H2O = (9Z)-octadecenoate + holo-[ACP] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15057"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9924"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78783"/>
            <dbReference type="EC" id="3.1.2.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 21">
            <text>a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:13097"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="4.2.1.59"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 21">
            <text>a 2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP] + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10240"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 21">
            <text>acetyl-CoA + n malonyl-CoA + 2n NADPH + 4n H(+) = a long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP(+).</text>
            <dbReference type="EC" id="2.3.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 21">
            <text>holo-[ACP] + acetyl-CoA = acetyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41788"/>
            <dbReference type="Rhea" id="RHEA-COMP:9621"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78446"/>
            <dbReference type="EC" id="2.3.1.38"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 21">
            <text>holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14 21">[Alpha(6)beta(6)] hexamers of two multifunctional subunits (alpha and beta).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 21">Belongs to the fungal fatty acid synthetase subunit beta family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.86" evidence="21"/>
    <dbReference type="EC" id="4.2.1.59" evidence="21"/>
    <dbReference type="EC" id="1.3.1.9" evidence="21"/>
    <dbReference type="EC" id="2.3.1.38" evidence="21"/>
    <dbReference type="EC" id="2.3.1.39" evidence="21"/>
    <dbReference type="EC" id="3.1.2.14" evidence="21"/>
    <dbReference type="EMBL" id="HG316464">
        <property type="protein sequence ID" value="CDF91412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5417908at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005835">
        <property type="term" value="C:fatty acid synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019171">
        <property type="term" value="F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004313">
        <property type="term" value="F:[acyl-carrier-protein] S-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004318">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016297">
        <property type="term" value="F:fatty acyl-[ACP] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03447">
        <property type="entry name" value="FAS_MaoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.70:FF:000001">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000017">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000078">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.100:FF:000001">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.366.10:FF:000006">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.366.10:FF:000007">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3330:FF:000001">
        <property type="entry name" value="Fatty acid synthase subunit beta dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.366.10:FF:000003">
        <property type="entry name" value="Fatty acid synthase subunit beta dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.60.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039569">
        <property type="entry name" value="FAS1-like_DH_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016452">
        <property type="entry name" value="Fas1/AflB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013565">
        <property type="entry name" value="Fas1/AflB-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041099">
        <property type="entry name" value="FAS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040883">
        <property type="entry name" value="FAS_meander"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003965">
        <property type="entry name" value="Fatty_acid_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050830">
        <property type="entry name" value="Fungal_FAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032088">
        <property type="entry name" value="SAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982:SF21">
        <property type="entry name" value="FATTY ACID SYNTHASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982">
        <property type="entry name" value="MALONYL COA-ACYL CARRIER PROTEIN TRANSACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08354">
        <property type="entry name" value="Fas1-AflB-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13452">
        <property type="entry name" value="FAS1_DH_region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22235">
        <property type="entry name" value="FAS1_thioest_ins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17951">
        <property type="entry name" value="FAS_meander"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17828">
        <property type="entry name" value="FAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16073">
        <property type="entry name" value="SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005562">
        <property type="entry name" value="FAS_yeast_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01483">
        <property type="entry name" value="FASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 21" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6 21" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 21" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 21" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 21" id="KW-0456">Lyase</keyword>
    <keyword evidence="13 21" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9 21" id="KW-0520">NAD</keyword>
    <keyword evidence="7 21" id="KW-0521">NADP</keyword>
    <keyword evidence="8 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 21" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Malonyl-CoA:ACP transacylase (MAT)" evidence="23">
        <location>
            <begin position="1671"/>
            <end position="2024"/>
        </location>
    </feature>
    <feature type="active site" description="For acetyltransferase activity" evidence="22">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <feature type="active site" description="For malonyltransferase activity" evidence="22">
        <location>
            <position position="1814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00058855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRNR" id="PIRNR005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR005562-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="CDF91412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="2056" mass="229627" checksum="74AB0A172E7E4C33" modified="2022-05-25" version="1">MSPPLSGSTRPLVLTHGSLEHVLLVPSQSFFSAHELLDQFKKTLPEPTEGFASDDEPSSPAELLAKFLGFLAEFASIDEAHYGPVLNVALNEFENSYLQGNDVHSVAARLARDQEVFPTTLAKAKEVVKSHVAARAALHKPFTKSQSALFKSAAQGESQIAAIFGGQGNTDDYFEELRELYQTYRRLIGDFIKFCADTLGELVKSTLDTEKVFTQGFNVLEWLENPGKTPDTDYLLSIPISCPLIGVIQLAHYATTARLLGFTPGELRSYLVAATGHSQGLATAVAIAEADSWDSFFQAVRKAISLLFFIGVHCYLAYPNTSLPPSILQDSVENSEGTPSPMLAITNLTKEQVQDYVDKTNSHLPSEKHIVISLINGARNLVVTGPPQSLYGLNLTLRKAKAPSGLDQSRIPYGERKLKFFNRFLPVASPFHSHLLSPAKPFIAEDLARYGIEFKQDDLKIPVYDTYSGEDMRNEKGSLAQRITDCIITLPVKWELTTKFETSHILDFGPGGASGLGVLTHRNKDGTGVRVIVAGTLENNMEDDYGFKQEVFDVTSNGVKRNANWLKEFHPKLVKNKAGQVFVDTKFSKLIGRAPLLVPGMTPTTVSPDFVAATINAGYHIELAGGGYFSPEGMTKAIDDVVNNIKKGYSLGINLIYVNPRMLQWGIPLIQKLRKRGYPIQSLTIGAGVPSLEVATEYIETLGLTHLDLKPGSIDAISQVISIAAAHPKFPIVLQWTGGRGGGHHSFEDFHAPMLQMYSKIRRHPNIILIAGSGFGSDEDTYPYLTGEWSKKFDYPPMPFDGVLFGSRVMIAKEAKTALAAKELISSCPGVKDEGWQQTYKKPAGGIITVRSEMGEPIHKIATRGVLLWRELDDTVFNLPKNKLQGVLDAKRDYIISKLNSDSQKPWFATVDGEIRDLTSMTYEQVALRLVELMFIRSTNSWIDTTLRNFTGDFLRRVEERFTKVKTPSIVQSYSALDNPDPVVESVFAAYPAAKDQYLNAQDIDFFLNLCQRPVQKPVPFVPVLDHRFEFFFKKDSLWQSEHLEAVVDEDVQRTCILHGPVAAQFTKKVNEPIKDILDNIHQGHIRRLLEAQYDGQESKIPVVEYFGGETPVMVTKPIAQPEETSDGKQVFKADAATDPATWFKLLAGPEKNWRNAFFSVAEFVQDSWFTENAAQKVFKPADGMVVEMTNPENPSKTVITLFEAIQVELKSVAVLKLVKDNLIQLELIENRTRDGNPVSLPLKYYYQPEDGFAPICECMEGRNNRIKELYWKLWLDEPCDLNFDPRKVIHGSQDVKITRNGIREFTHAIGNNCEDFMPRSGKKLLAPMDYAIVLGWRVIIKAIFPETVDGDLLKLVHMSNGYRMIPGAKPLQEGDVISSSAIVKSVVNMPTGKKVEVVGTLTREEKPVMEVTSSFFYRGRYSDFENTFQKTVDPVYQVHINSPKDIAVLRSKEWFQLDDEDIDLLGKTLTFETETEVTFKNANVFSSVRCSGPIKLELPTTENVEIGIVDYEAGESYGNPVIEYLKRNGSTLERKVNLENPIPIGVLELQSPSTNEPYARISGDLNPIHVSRHFANYASLPGTITHGMYTSAAVRALIENWAADSVSSRVRGYSCKFTRMVLPNTSLTVSIQHVGMINGRKLIKFETKNSEDVTVLVGEAEVEQPISAFVFTGQGSQEQGMGMDLYQNSEVARKVWDRADIHFKETYGFSILDIVRNNPTELTIHFGGEKGRKIRNNYSSMIFETIVDGKLKTEKIFKEVEEDSSSYTFKASSGLLSATQFTQPALTLMEKASFEDLKSKGLIPSEASFAGHSLGEYAALASLADVMSIESLVEIVFYRGMTMQVAVPRDELGRSNYSMMAVNPSRVSPTFTQAALQFVVQKVDSRTGWLVEIVNYNVENQQYVAAGDLRALDTLNNVLNFIKLQKIDIVKLQETLSLEKVEEHLFEIIDEISKKSAAKPRPITLERGFACIPLRGISVPFHSSYLRNGVKPFKNFLKKNVKKENIKPERLNGKYIPNLTAKPFEITKEYFQDVYDLTGSEQIKEVLDNWEKYEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TB08</accession>
    <name>A0A8J2TB08_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Transcription elongation factor Spt6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_03818g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Histone H3-H4 chaperone that plays a role in maintenance of chromatin structure during RNA polymerase II transcription elongation thereby repressing transcription initiation from cryptic promoters. Mediates the reassembly of nucleosomes onto the promoters of at least a selected set of genes during repression; the nucleosome reassembly is essential for transcriptional repression. Essential for viability.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Plays a role in maintenance of chromatin structure during RNA polymerase II transcription elongation thereby repressing transcription initiation from cryptic promoters. Mediates the reassembly of nucleosomes onto the promoters of at least a selected set of genes during repression; the nucleosome reassembly is essential for transcriptional repression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the SPT6 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316461">
        <property type="protein sequence ID" value="CDF90819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="995477at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09928">
        <property type="entry name" value="SH2_Cterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09918">
        <property type="entry name" value="SH2_Nterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2740:FF:000002">
        <property type="entry name" value="Transcription elongation factor Spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.650:FF:000007">
        <property type="entry name" value="Transcription elongation factor Spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.850:FF:000001">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000056">
        <property type="entry name" value="Transcription elongation factor Spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000065">
        <property type="entry name" value="Transcription elongation factor SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.650">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2740">
        <property type="entry name" value="Spt6, Death-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.850">
        <property type="entry name" value="Spt6, helix-hairpin-helix domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3500.10">
        <property type="entry name" value="Tex N-terminal region-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="entry name" value="YqgF/RNase H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049540">
        <property type="entry name" value="Spt6-like_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028083">
        <property type="entry name" value="Spt6_acidic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042066">
        <property type="entry name" value="Spt6_death-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032706">
        <property type="entry name" value="Spt6_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028088">
        <property type="entry name" value="Spt6_HTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035420">
        <property type="entry name" value="Spt6_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035018">
        <property type="entry name" value="Spt6_SH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035019">
        <property type="entry name" value="Spt6_SH2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028231">
        <property type="entry name" value="Spt6_YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055179">
        <property type="entry name" value="Tex-like_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023323">
        <property type="entry name" value="Tex-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023319">
        <property type="entry name" value="Tex-like_HTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017072">
        <property type="entry name" value="TF_Spt6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006641">
        <property type="entry name" value="YqgF/RNaseH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145:SF6">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14635">
        <property type="entry name" value="HHH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14641">
        <property type="entry name" value="HTH_44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14633">
        <property type="entry name" value="SH2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14632">
        <property type="entry name" value="SPT6_acidic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21710">
        <property type="entry name" value="Spt6_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22706">
        <property type="entry name" value="Tex_central_region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14639">
        <property type="entry name" value="YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036947">
        <property type="entry name" value="Spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00732">
        <property type="entry name" value="YqgFc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158832">
        <property type="entry name" value="Tex N-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="SH2" evidence="13">
        <location>
            <begin position="1256"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR036947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF90819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1450" mass="167698" checksum="2CDF19CAE5F0EABC" modified="2022-05-25" version="1">MSQVDQGKRELLASRDEDVIANGDEDKPPSDEEEGEDVFDSSEDEEGEDDADEERRVREGFIVDDDDEDVITGKGSKKRRKHKRRVKEQDDDGRLSEDDLDLLMENAGVKRTTDQASSGRLKRLKRAGQEASPDENSAANSKEPTDSRLDDFFSDEEGEEQTAGDDERRGERAHGEDEVDQHGDRDGHKGGILDELDDFIEDDDFSDEDEETRQQRIQERKMLREQRMKQPVHVTGLSSDKIDEMYDIFGDGHDYDWALEIENEELDQGALEDGAADGEGDGEELGGGNKPVRKKITLKDIYDLQELKKNLMTDEDLAIRKADIPERYQELRAGLANYNELSSEDQELEKNWISDKIAMDKNFAPTYDLTEFRECVGNAIQFIAQRNLEVPFIYAYRRNYISSRDADGFVLTEDDLWDVVQLDIEFHSIIHKRDYVKRFYLELHVNDPVVDEYFKNQTTASTAELNSLQDIYNYLEFKYAHEINDSLLKNPNKKHLKNSSYEKFKASALYQAVNDIGITAEQVGENINAQHQIHVAVDHPNLKPEEVIASILNSNTADLQVFTSNTKLALDTVKKYYAMEISQNTKVREKVRSDFYKYYLADVVLTSKGKREIQRGSIYEDIKYAINRTPMHFHKDPDVFLRMLEAESFNLLSVKLHMSSQSQYVDHMFQIALETTNTSELATEWNNFRKSAFTQAMDKIFADISREIKDDLSKTCEKLVAKTVRHKLMTKLDQTAFIPNPKDPKIPKVLTLTCGQGRFGSDAIIAVYVNRRGDFVRDFKIVDNPFDRNNPQKFEDTLDTIIQSCQPNVIGINGPNPKTQKFYKKLQEVVHKKQIIDSRGHTIPVVYVEDEVAIRFQNSERGSQEFPNKPPLVKYCIALARYLHSPLLEYANLTLEELGSLAIHPHQMLIPREVLLKALETAFVDIVNLVGIEVNKATDNPYYANALQYISGFGKRKAIDFLESLHRLNEPLLARQQLITHNILHKTIFMNSAGFLYISWNEKRQKYEDLEHDQLDSTRIHPEDYHLATKVAADALEYDPDAIAEKEEQGTMSEFIETLREDPDRRSKLESLNLEQYAEDLEKNTGQRKLNNLNTIVLELLNGFEELRNDFHPLHGEEIFQSLTGETEKTFFKGCIIPVRVERFRHNDIMCITNSQVDCIVNAQHHMGAQFKRPPGDIYEIGKTYPSKVVFIDYENISAEVSLLEHDVKHQYVPINFSKDPSVWDLKQELEDSEEEKKITMAEARAKRTHRVINHPYYFPFNGRQAEDYLRSKERGDFVIRQSSRGDDHLVITWKLDKDLFQHIDIQELEKENPLALGRVLVVEQQRYHDLDQIIVEYLQNKIRLLNEITSNEKFKSGNKKDVIKFVEDYSKVNPNKSVYYFCFNHSNPGWFYLMFKLNAQSKLYTWNVKLTHTGFFLVNYNYPSVIQLCNGFKTLLKSSSSRNKTATYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TBX9</accession>
    <name>A0A8J2TBX9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ZYBA0S10-02058g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_02058g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RICTOR family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316463">
        <property type="protein sequence ID" value="CDF91205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="271111at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028268">
        <property type="entry name" value="Pianissimo_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028267">
        <property type="entry name" value="Pianissimo_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029453">
        <property type="entry name" value="Rictor_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029451">
        <property type="entry name" value="RICTOR_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029452">
        <property type="entry name" value="RICTOR_V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13298">
        <property type="entry name" value="CYTOSOLIC REGULATOR PIANISSIMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13298:SF11">
        <property type="entry name" value="RAPAMYCIN-INSENSITIVE COMPANION OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14663">
        <property type="entry name" value="RasGEF_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14666">
        <property type="entry name" value="RICTOR_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14664">
        <property type="entry name" value="RICTOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14668">
        <property type="entry name" value="RICTOR_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01303">
        <property type="entry name" value="RasGEF_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01307">
        <property type="entry name" value="RICTOR_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01308">
        <property type="entry name" value="RICTOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01310">
        <property type="entry name" value="RICTOR_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR N-terminal" evidence="5">
        <location>
            <begin position="283"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR middle" evidence="4">
        <location>
            <begin position="692"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR" evidence="6">
        <location>
            <begin position="1107"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="175"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="140"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="106"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF91205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1372" mass="156477" checksum="BE8A0ED252FFE580" modified="2022-05-25" version="1">MSVVHSNPHGSPKDERARSRSSTTNTSNTDGSGFLNGDSNSSIHNNKKQRQKLVLSTSFLSTKRLDSVPPSPASPLQGKRPTTSLNKALQGLKAEINQLQQQLNETRRNKEEAEHIRDSTGSEIYSGTYSTDHLQKHSMRIRANTQIRELDKAIKRIEKQLTDLKNQFEHTKKSKELYAARDHPKWLPREDLSPDGGTSQDSSKELSLEQTLMAHDSPLPLEDAHTLRSSPDKELESDEHASDILSQSDEIEPNAKNIETATWLVSGYMQSLQDASSSADLVLKRSNGLVALLKQHPEIRSNLLLDSFMYTVQSMLLHEDRIIVSCGYRICRYLIDGEAFVQELLRLHVDVCIIISFAKDFSFQMEREQALRLVRTFLEHRAGITKGIVQAVISCVEKQDDPLRNMALETLLELCFVSPDVVSECHGMRVLESILQECSFFPLASVILDTILQLMSMHATRVHFLDDFDISILLTCFSDSNSRSALNVEKMQNACLLITKTLKDYNGFMLFSMDNFKPLKELLSFFQVPMCAQYLVDIFFDVLRIKQLSYDKNKSHFKQISMHFSRESMLINQHLALLVLMMYRSDFVKHIWDLIDQTKDHKVATSLISKSRLLLTEFMNLASNLLDLNVFDGTGLPDSFDPLIYDETFQLGKITYSMNRSRNKLGIAGIDYGKPVKSFSQNMSVGTLGREVDDIKFRKMVFDSKVLQTKDFTVWNWNVIQELLEGPLLNAKLLDELVKSTKFIRRLLIFYRPLRMRFANVNHGTRLSHRYVQAGCQFFHMLTASSVGMRILMDDTKIIPQLASLLFRAMEGHTAGNIFNARSLSTKMVIGYFKFIGVLTQSANGISVLTRWNFFTVIYKMFQPHSKLSSKFLLSTLPELDLRYSPLCRTIMGKALVNPDTHIRVKATEYLGEKLKDLFDFDDSQNSSNLEKGTILRRYIMEMLTRQLYDLSPRVVAIADQALYEYVVKNESSQGLGSSLQTSLNQLVFIRSPIIFELLGTTYGFQLLNEINFVQEERKSWLASKNKEYVFLVENFLNYNQHGTFITSSRKMAGMDHLPQHFYESLAKTEDGIALIGQSRDLTTFISVLKAFSQTLDSAEDPPIKDMIQLKSALWCVGYIGSTELGVSLLDEYAVVEDIIQIAYYASVTSVRFTALLTLGLISKTAEGCEILDEMGWSCCLNVQGSPMGITFPSRLDRFLSYNEKRWSSQGDYKEPVIEFNTQVGGIIGEVQPVSLNLSVLLNEKNMIENPLYDGSLQDLIKTEERYYIKSAMSEKRQYTNEEEKTIDEVLTIVSQLGNHILSGNAIKGITEMNKRYGPSLFESETMFYRILDMMSKYRFKPHVRKFLCGLFINKKVLENVIRNERRRTNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TC75</accession>
    <name>A0A8J2TC75_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar membrane-associated protein IML1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Vacuolar membrane-associated protein iml1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN860_04126g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IML1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316463">
        <property type="protein sequence ID" value="CDF91296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="39497at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04449">
        <property type="entry name" value="DEP_DEPDC5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045838">
        <property type="entry name" value="DEPDC5_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027244">
        <property type="entry name" value="IML1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048255">
        <property type="entry name" value="IML1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13179">
        <property type="entry name" value="DEP DOMAIN CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13179:SF8">
        <property type="entry name" value="GATOR COMPLEX PROTEIN DEPDC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19418">
        <property type="entry name" value="DEPDC5_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12257">
        <property type="entry name" value="IML1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEP" evidence="6">
        <location>
            <begin position="1138"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="732"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1262"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="741"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="752"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CDF91296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1528" mass="175099" checksum="627F528CAE5BE94B" modified="2022-05-25" version="1">MAGNGPLFAGLRAKNQRPVTSTVSTGPRANNTTHANSISLSAGNLILGASDKTNHRANFIRGPSGTFQAVNSGNDNIPEGAQPEAAKIHDKNRSKQVELCYHESRYSNEIVEIDLAQIPGAKEGDLFELKTYQKVPGGRDKKIYFIAKDFNPEIRSRVKNAQLSILSGQLQSLLDLPSRSKVWIKLKSKTLTEADLVELNIKDCLINRGDMWCINAELVNHCVFSGQKMTFLDTIRASVNGIYREGKKTLSGYIGENTRVVFRSESARLIFLIQITDEMWNFEETGEQLFQKMVNSFFPKIFKKWKDIGTHHSITIAFAVSMDTSQVPFRELEPGTILKNTTDYFRIVVDQVLVIHWVEIMETLRKEFQALRRNLLNVKTEEGYSVIKGRFSPVIKSNILELVNFASTLITDPFRQSDLRHTTTHVMIISPGSGLYDVDYDLLKLTGKKLLSLEMTMDLICLSRAPLHVVPLFRYLDYENRIHHCSPTWLSIFFWNDSIKNSDDWRPRCKIYDLQMMGLTENDVLGEVDIEHLMPSKGEKSIQQFMDQYDKDVFSFRRSEGFEKIENLQNIKVDNGKLSNVNPTLQPLSSFNWNAPKFATPALKDIQKPKVFADLYTQKSNRSTTASDEERSDSVLLGESAFKTQQSLAVNTLKGIGKKNLVKDLTQKIVNSIIPDRDLNKGNYFEEEVHRMNENEIESTLTPRKSISPVNKYRENGPIIKKNLSVLDRLDSQSNSIPEDSSGRESAHTSSKDLTFSETNPFISKDDKKTSIQESVSQVEDSSPMGVKTWIEIKNPSVPVSETIAGMMFPMRWKDVLPRYVPKRYSKWRSFTTPAELPVTISDFPSKLDFETNFFFRNHSVNLNIDQEAFKQTYKDLLRNMIYMRLLAGFQLCVGENAKEVERSKGMENGESPIAKFIDGSWVNIKIYMMMDSEIHRISCGIDGVVDVQRYLRKNEENPFEQVSSYTPLVKTRYENEYRKAKIDPLHAARKTLNWNQIDQVLAGYGDYGVDKNTCGFRSKLVVLPADIPPNTYSSVINGRNETLTPEEIRLEGLSRLISSISRSRLLSSKEEQARKNKKQEIQPEILFYTGSLFDFIREQKSSLDKSVLSYKDSIFADRKQLRKDVDIWELARELQHGENSLALVNRKWHWKRHQNSFIGSELVNWLLRNLSDIATREEAVAYGQTLMDKGLFKHVLNKHGFLDGHYFYQIIPPYLVDPRVVEKASSDPKTNDNKRFMLDNSSSNFAEINILPLTMTTSNTNSNLSADNQSEVKNNSSSSKSSVMLSNSLVINVDPAGKSYKLETCTVHYDKVHNPDHCFHIRLEWLTTTPKLLDDLVANWSRLCERYGLRLVEIPWRELCMIPAIDPFHSFVEIHLAINPWKDPEFRDEELLSRSKFYYHMYLLKVSGFLLDNRASKVIQDDDCAFEIMYSWGKPEFKYAQYIHITGAYIAEVRESGDLFLAPNNVYISRANPGKIVGKSQFSTQFALDAQKVMLSFKKVCTDYNELRSIFFDAKEKWVRDQQIDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2TD74</accession>
    <name>A0A8J2TD74_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ZYBA0S12-02300g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_02300g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The VLRF1 domain mediates binding to the 60S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ANKZF1/VMS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316465">
        <property type="protein sequence ID" value="CDF91585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2TD74"/>
    <dbReference type="OrthoDB" id="429841at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047139">
        <property type="entry name" value="ANKZ1/VMS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041175">
        <property type="entry name" value="VLRF1/Vms1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16036">
        <property type="entry name" value="ANKYRIN REPEAT AND ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16036:SF2">
        <property type="entry name" value="TRNA ENDONUCLEASE ANKZF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18826">
        <property type="entry name" value="bVLRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52044">
        <property type="entry name" value="VLRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="VLRF1" evidence="13">
        <location>
            <begin position="225"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="113"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="278"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="608"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="542"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="613"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF91585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="636" mass="72484" checksum="D10B23CB6164D21C" modified="2022-05-25" version="1">MSMVKKNELYVYELSAPVLRSLNLMVFDATLREVEPPLRGANDDTESAYSSSNANNQVHSNHLHCNLCRLEFTDQKTRREHFKTTFHIFNVKRNLKGLSSVDEPELERILQQTNEVQTDEFSSGESSSNEDNGEEESEDSTEESMDIILEQTLEKELEDISLTSSEFTNASVISHLATRSSQIYFKSQLLSDSEVFGVYKSLFTQGSIKNPLSSIQAWNELEDQGISISAMFMMGGGHFAGAIVSHQRINVHGNTKKQDESVQEQQVNFIEHKTFHRYTTRRKQGGSQSAMDNSKGKANSAGSSLRRYNEAALRVDINNLLKEWKPYLSKCQNIFLRSRNSQDRKLFNEIISQGKPDPRLKSFPFTTTRPSIKELKRSWCELTYLKIVPKPKPLPSAVTSTTAAKASSKRAQKLEERSAKILSAEEKHTEHLLSLLDKGRAPLIIAYLRKNGIDVNFKLAPINHHVVAPTMLHYASQQGLKQMVIILLTTIKADPRVKNNLDRTPWDLAKTAQTRYSFQIARHLLGESFTDWDEAHVGEPLSREQVDELNKQEEDRENLAAQVAMRKELDDIKEKQKLEEEKHRLENVAKKGLGRVLDSSSVIREQNLNSLSDEQRRRLMREQRARAAEARMAARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WVP8</accession>
    <name>A0A8J2WVP8_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BN860_18800g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_18800g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2WVP8"/>
    <dbReference type="OrthoDB" id="823504at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050776">
        <property type="entry name" value="Ank_Repeat/CDKN_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201:SF15">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="108"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="141"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="284"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="298"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="322"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="352"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF87976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="399" mass="44204" checksum="DFB644BEB32DFEB3" modified="2022-05-25" version="1">MLADPSIRLREAIIEGNLLVVKRLLRRFPELLTNIDPKNGWSSLHYASYHGRYLVCLYLIQLGHDKHEVTTTFKGNTCAHLSLMNGHEQTTHLLLQYFPRFIDWSGDRGKTPVHIACQHDHFQCFNLLMGVGANLTMKDDYGDTPLHVCLEYGSVNCMKMLVLQGGIVDDNVKNKKGWRPSDVAATYEMARVYAKLLKEAQIPGIPAKPPYSSFRTPTQGSKAVFDDGPSPVLAMNSPHSLYPHVGNGIPHLPKISTSRRASLVNSSKSPVSNIASWFASKDSSLNNSSGSLHRRDTLNSSTDRQTRSSTEISPSKRDSQRGRSSGSHTRASSHGNTSDLVNKYLLLGSNENHENPIVNHEDRSESAHASPRIGRRRISLLNVPIAKLRDHESDSSVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2WZ29</accession>
    <name>A0A8J2WZ29_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ZYBA0S03-12662g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_12662g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle</location>
            <location evidence="5">COPI-coated vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="18431at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030663">
        <property type="term" value="C:COPI-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000003">
        <property type="entry name" value="Brefeldin A-inhibited guanine nucleotide-exchange protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.20:FF:000002">
        <property type="entry name" value="Brefeldin A-inhibited guanine nucleotide-exchange protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015403">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046455">
        <property type="entry name" value="Sec7/BIG1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF375">
        <property type="entry name" value="LD29171P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20252">
        <property type="entry name" value="BIG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09324">
        <property type="entry name" value="Sec7-like_HDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SEC7" evidence="7">
        <location>
            <begin position="786"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="730"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1697"/>
            <end position="1728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1745"/>
            <end position="1778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="71"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="130"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="170"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="185"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="299"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="731"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="758"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="770"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1745"/>
            <end position="1765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF89247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1981" mass="224883" checksum="4B101A63129A2EA7" modified="2022-05-25" version="1">MSDRELAAGDSAQDTVTSIAPAASTVSLSLDSSPEPGPEVPPQLDEPEEAENEPEEQEAVEQEQEEEEEQKEVKQEREQEQQERKEMQGLKEEDVQKKEIEQERTLXXXXXXXXXXXXXXXXXXXXXXKPEQEQKPEQEQKPEQEQKPEQEQKSEQEQKPEQEVVNGQDEAQHSPKIDDREETAAPTTRSSVGGSNAKSTIALVRETLKVISEDKDIKKYPNVQKVAERTLAKLQELSAQGSSGPYLLDSIAIFEALRSCCRTKSTRIQLKALDCISKLFSFRALDEAILVNPPDAMASNDQTQEDATTGITPPPKQRLIDASIDTISDCFQGEGTDERIELQIVSALSSCILTEDSYSLCHGASLLKAVRTIYNIFIFSLSSSNQGIAQATLTQIVGTVFDKIDLKQLPAYSSVDSQEIDDNESVVQHPPLTLQNMDVLNEDEDQAMEDTSKMAQTGELDEQTLQELLIKDAFLVFRAMSKISAKPLETELDMRSHAVRSKLLSLHIIYSIIKDHIEIFLCRTAYLPGKERVTLLDSMRQYLCLSLSRNAASSIAPVFEVTLEIMWLIISNLRAEFKRETPVFLTEIYFPISDLKTSTLHQKRYFLSIIQRLCNDPRTLIEFYLNYDCNQGMPNIMEMMVDYLTRLALTRVEITPTQRSHYMEQLSKPLATYNVNQVPLLSTSNLSSSFDSNQPNLQFPVEFALKMTSLNCIVSVLRSLSSWAHRALNPTVHNNGNNQTGRPVSGISAGPRRKDSSFHGSSISLNSIGGEEDESMVSQEVDDPSQFESLKQQKTELTACIKLFNSKPKRAIPELITKKFLRDDSPKSIAEWLLATDGLDLATVGDYLGEGEEKNIEIMHAFVDEFDFTGLSIVDALRNFLQKFRLPGEGQKIDRFMLKFAERFTDQNPGIFSKADTAYVLSYSIIMLNTDLHSSQIKNKMSLQEFLENNSGIDNGKDLPQEFMVNLYNEIANNEIKLLSEQHQAMLTDDGNLIHQQQQQQSAFNFFSSRDLVREAYMQVSKEMSSKTELVFKNLNKSRGKGNTDVFYAASHVEHVKSVFETLWMSFLAALTPPFKDYDDVDTTNKCLEGLKISIKISATFGIEYARKSFIGALVQFCNLQNLEEIKIKNVNAIIVLLEVASSEGTFFKESWKDVLLVISQVERLQLISKGIDRESVPDVAQARVATQRVSFDSTRSNSASFFDKWTKRATPLELAQEKHYNQALSPEISKFISSSELVVLMDHIFTRSSNLSGNAIVDFIKALTDVSLEEIESSQDASTPRMFSLQKMVDVCYYNMDRIKVEWTPIWAVMGTAFNKIATNPNLAVVFFAVDSLRQLSMRFLNLEELSGFEFQHDFLKPFEYIIQNTSSTDVQEMIIECFRNFILTKSSKIKSGWKPILESLQYTAKSSREGIIYKTYMLATNDIVANHFESVFCQEDAFSELVSVFKEITKNKKSQKLSLHALEGLRKMTQKIAEICFYKGSDEPKRTHYEAVLRGKDVFQNIWFPMLFCFNDTIMTADDLEVRSRALNYMFDALVAYGNEFDDDFWEKICTKLLFPIFGVLSKHWEVNQFNSHDDLTVWLSTTLIQALRNLIALFTHYFKSLNKLLDGFLGLLVSCICQENDTIARIGRSCLQQLILQNVAKFNDAHWQDIGGVFDRLFELTTATELFEYDPMHQGRRKSVSHHYRRVLSQGNTIKDASANGNGKEETNGNIGETVQRAHEEESSEDVGNDMIAEGSEIAHNAKQTNSPQLGKQNVTTNNQSKPSKRLVHTKSSEEMRHRISVKNSIVVKCVLHLLMIELLSELFENEEFASCIPHKQAIQVTILLEKSYEFARDFNEDYQLRTRLVEARVVDKIPNLLKQETSAAAVLINILFKLYLNDDQKKPDLGKRLIDICTSVVEGYVALDDRMMERSINSWRPVIVEILQGYYEFDDDDFKEYCPAMYGLVMLMLDKSVPADLRKAVKVFLSRVGELYLEVHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X3F5</accession>
    <name>A0A8J2X3F5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ZYBA0S08-04874g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN860_04874g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NUP186/NUP192/NUP205 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316461">
        <property type="protein sequence ID" value="CDF90860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2019644at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021827">
        <property type="entry name" value="Nup186/Nup192/Nup205"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11894">
        <property type="entry name" value="Nup192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CDF90860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1671" mass="189425" checksum="42BF8F2B6DCE9EE0" modified="2022-05-25" version="1">MRWSATDFQSLYYCIETGNFDGALYQDLLPDLQNLNLNDNKLKNATSRSQLEKGEVTLENGNTYKINQQFMVAVVALSDELNLDELVVCELILSDPSTTNGENDEDLALVSAGKIQYFLRRQFILQIVAFIVNCADTDNLVYRDLTSNGALAENILNAFKFIHTQLADIKQFVNRAQILDNYNALVQQNVKFKRDFLLREYDILSQILYGLMSKGILMKKDRIMQILDFASELDPNDFFITYYLPAVLHAFHELNHLQDSEVRDMHTIFIKELKNEAIYAKPVKVALIFIFLSYFIGWCKQEPSKRADSFDFPTAVDEPMTAAVELGAIEQLLVFAADTSEVGKDKSLDLFYDLRSLLERHIPRLIPKQLLDSEGEIQGDATSATINSALPSANDQFQHIVFSEQTQGFFLSAFDEALRTTIADCAFLLTKIKDAEEDSLLSGEDLDLDDISVKADLERFFLTIYFFYSYRPEFAKSFWQDKESNAYGFIEWSAKCSDNLMRSCFYLMISSLSYGEENSLSAYHYIGGNNSVSWKAISQCIGDYISRINKLTEKMHQRQQKQESGEMDLTAVAFEEGLNEETVIFISPLLTLIGTVAFNVDKEVKRALSGMFTDLLFDFAKLDTPLIGACFKTLSELVPKLESERSKFWYALDSLIFKKSSLISFQDSYRDVFTSALTNFSEVVGFLQLFGKLISIESKEPDTNYMVFGKLAFPIKLGASYRKAGVWPYFDYIFNEVFVHSTKLHQTWRIRSVQMPILQIIEESLFSFNYNVILDSMSAGVNLDNLVETEDFFTYIQECPATAVFNYLFKEKCYKCLFEISDVGVDHISIDLGSGAEQLQLVDLAVKIINEVLANQETYIEELSPIFMKHGKSEYFIPKNFGLHGLRSFYDAIFFNLPTVACFGLYVGVDDYVLASNSLQILDKIAARHSNEDTQLAVKNKLLIIFDSVDESARIKDAFISQVERSIDNAEMLKLKLEVLNFISTNLSYTDRRFTVAHLLLGFQVTNILSVGPNLSTFVGSGTSLLSSLISLLESSLQAMTGDQIDYAPMRLAAAALEIILKLCRNPLTSTLVLDYLAEKQLFEKLLELDLPVNNCTSWNGRLFDPKFPENTKEFVQTESIGSLLSFLSYRNYFLQYMSLAIHHLSKSGRKSQINSHIELLISNTIYSARILSLLDSLNLGDLSLDYSPIKSLELFANLPLNLEKVNFRTSCTGNIFDFSEIRSLMNLSQRVYTMSPVDNASTMPKSGKVAKIEEQAKTESDVICQCTTSYLCRKKFTEGQLAILHSWVQLVQITVSDGELSALTRSNFILEVFGSVVPKINDYIEFHISFSEELVSLAVFLYDVYRKDRMAIDNYQSVDTRLYNLFKVCVQGISSPLSSFSLRSDFYLLTNSYLVRILKDSKLAKQVLQDLKMNYEVLVEIVCNDAIYGQGTNRITGIFLLDSLIQLGNLNKENFILDSLGKNTQLLLIIRTIKNVDTMLSSPSENISIDYLLYELTAFKSILYLLIRIAESRNGAHALIQNKLFQVIRDCKFLEIDPDLGLELVFDEMSIKSSSFLRVNVSLDNPLFLGKDTDGISLFELIVPVFQLISAVLLSMGSANQPVIMEVKKMLIKFRKLSLGILKRDALKDINSKYHLSGPNSEGLEKMVRLIVMLCTLTGYHGEEKKLEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5S9</accession>
    <name>A0A8J2X5S9_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_02256g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_02256g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5S9"/>
    <dbReference type="OrthoDB" id="498590at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039662">
        <property type="entry name" value="Cohesin_Scc3/SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048610">
        <property type="entry name" value="SCC3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020839">
        <property type="entry name" value="SCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013721">
        <property type="entry name" value="STAG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199:SF0">
        <property type="entry name" value="LD34181P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199">
        <property type="entry name" value="STROMAL ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21767">
        <property type="entry name" value="SCC3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21581">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08514">
        <property type="entry name" value="STAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51425">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCD" evidence="2">
        <location>
            <begin position="337"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1024"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1024"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1044"/>
            <end position="1058"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF88098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1073" mass="122707" checksum="492A22543C72833F" modified="2022-05-25" version="1">MSEVRRSSRIRTKSYENASATEGGTKDEQILNGSDGESDSENESESEGDIQDDEDYEEPSKKRRADSNSKVASRQKRAKSAGMSRPSTTSKQEQETYLEAIKDFQPTELFEILSTSEDVSIDELLRNWLESYSTDRDDFLQDFINLLLCCCGAIARLEAHDVHSNESSNETVGELQLLFQKQKIHEFHLLISKNNKKKTKYPHLYANFVEFMSRLMDIANDLQLLYVESDQDESEISTGPLVLDLLTWLSPLSVCKIRSLRYVATLTLYLFQDFLADHVVDLDKNYLSKLSKQLAAENKKKKPNHKTLEKIESSIAEIQGSKMVTQGIIDNIIKLCFVHRFKDVDEVIRCESMIHLSTWIKSYPEYFLKVTFLKYFGWLLSDSSANVRLQVLRILPNLIVQNHRKAVDNSAVRQFFERFKDRILEIALKDVNLEVKLSAVNVLVEVASLGYLEDGEILRISSLIFEDNKVNISSHGRNSRYLGSVAKFISCITEERYQEFARNRDLPKSLFEVESSSAVKVGIFMSILNDSLAEHFREEAHSDAETKIRILFQAAEFLYPYFGSLIDDLCRMLIFEGDFTHPSLQATVEPEENNLTLLLPTDSNNLVLYVTTLHGLSYGGKYAKSQSRFKVTEAILPHLEKLIKHLPIESSNILSSVLGIFNLFSFEDWLHTGCEKDIRKIVEKIIKAFNETSLCSGPEDVKYKSFLEAVEHVRRLAFNELDELWLNHITYLKIHLVKFLEEKIQNSESNQGADDDRMVNALYGSFLNKLTLLGKVYPIEFEDKLLDLFLNGYVRRVPAIAAHCQLESVQQINFKLLSLLSTWQLQKWIDLLEKSSENTVPSQVPISSFKTVKALLDSFYSIFKVLASDLNDNDGTLEDFLLKWSAANSFIDILISLKVFELGVADSERSWKNALREQFPQYIQESANLVFLQVFLYLESLYANESSIQLNRNPEEDANLNDIKYAGFEGNSEKELLLFTIKLKAVMKLGVRLSNDHIATRISLNKEKLGVLYIKVIDDKSFEGSKEDKASGRQQLLKDHSNEELEPIDEQSQEDEPAEISMIGHDSIDDSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X5Y4</accession>
    <name>A0A8J2X5Y4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_09274g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_09274g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X5Y4"/>
    <dbReference type="OrthoDB" id="4069891at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="223"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="97"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="240"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="249" mass="28120" checksum="36C9ADA021E320EB" modified="2022-05-25" version="1">MASGLPPGASADATDEILIELKRLGDNYQALDDHVESLNKHTVRAQVDVEMLLNRSTNNNKHLQTMSQLGQSIDRVLQLVEKLPQGSEPSASHVSQEAEATHRLAELNQEIMEKERRAAALDADYEQMKERIRERHNEFIQLQKDYLEFQTTMQQQLKSTMGRVMVSTSALLDDADSTPSASSKMQRITSMLRERYKLENGSRRVVSTDSVLNGVQGRILPKKRYVLNPRSPHGSPMTPDRTSYASDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X674</accession>
    <name>A0A8J2X674_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="13">BN860_05402g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_05402g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. BMS1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X674"/>
    <dbReference type="OrthoDB" id="10260897at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01882">
        <property type="entry name" value="BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000105">
        <property type="entry name" value="BMS1 ribosome biogenesis factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037875">
        <property type="entry name" value="Bms1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030387">
        <property type="entry name" value="G_Bms1/Tsr1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF2">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BMS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22298">
        <property type="entry name" value="Tsr1_G-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51714">
        <property type="entry name" value="G_BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Bms1-type G" evidence="12">
        <location>
            <begin position="68"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="448"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="553"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="627"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1114"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="454"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="478"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="561"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="631"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="679"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="695"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1139"/>
            <end position="1157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF88238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1177" mass="134557" checksum="0750E98442BC50CC" modified="2022-05-25" version="1">MEQSNKEHRQRKEKNTAKKKLHTQGHNAKAFAVAAPGKMARTMQRSSDVNERRLHVPMVDRTPEEDPPPVIVAVVGPPGTGKTILIRSLVRRLTKTTLTELNGPITVVSGKRRRLTFIECPADDLNAMIDVAKIADLVLLMMDGNFGFEMETMEFLNIAQQHGMPRVLGVVTHLDLFKSQSTLRASKKRLKHRFWTEVYQGAKLFYLSGVINGRYPDREILNLSRFISVMKFRPLKWRNEHPYMLADRMTDLTHPELIKSQGPQVDRKVALYGYMHGTALPAATGTKVHIAGVGDLSVAQVEKLPDPCPTPYFQQKLDEYEREKLKSQGKTSGTTRRRKRLDDKDKLIYAPMSDVGGVLLDKDAVYIDVGKKNEGPSFVPGEEKGEGERLVTSLQSADKTYSEKFDGVGLQLFSNGAELHAKEDEEMDQDDEISNDRGRSTLRYARTHGKTVQEEDADVDELPSDEEIDEETAAENMRSSKLEEIDFDRDVDGGDGDERLALDSDSEFELSDAEGQNWEVTTAGKLGGSINRRRKWDIGKLVYMENISPRDCLRRWKNEDSGENEQESDIEQEDEEEDFFKKKDESGPVTLDYEGDMEIFTPYFEKLEKLVQKWSSLDSIKDRFIGAPDLNETENGESQEEGSNDEEVYGDFEDLENNEEELQDGSRVDESEDGVSGVEDNDGQSDDSFADFDEEEKKDAEQGKSLTVEEERQQNQAKKEKLRLQFEMEEGDNFKEDDPENEYDTWYELQKAKMAKQLEINNAELESMPAEKRQTFEGYKAGSYVRIVFENVPMEFVENFDSRTPVIMGGLLPTELKFGIVNARLRRHRWHKKILKTNDPLVLSLGWRRFQTLPIYTTTDSRTRNRMLKYTPEHTYCFASFYGPLCSPNTPFCGVQVVANSETGGGFRIAATGVVEEIDANIEIVKKLKLVGFPSKVFKNTAFIKDMFSSAMEVARFEGAQIKTVSGIRGEIKRALSKPEGHFRATFEDKILMSDIVILRSWYPVHIKRFYNPVTSLLLKSKNEWKGMRLTGQIRAERGIETPSNPDSDYKKIERVERRFNGLKVPKSIQKDLPFKSQVHQMKPQKKKTYMAKRAVVLGGDEKKARTLMQQVYTLSNSKQEKRKAKKQDERKERLKKLAKAEEESAQRDKMKKKEYFAVHGKKRSNEDNHGGSKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6M3</accession>
    <name>A0A8J2X6M3_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">Protoheme IX farnesyltransferase, mitochondrial</fullName>
            <ecNumber evidence="11">2.5.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Heme O synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_14356g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Converts protoheme IX and farnesyl diphosphate to heme O.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the ubiA prenyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="11"/>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6M3"/>
    <dbReference type="OrthoDB" id="5211at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13957">
        <property type="entry name" value="PT_UbiA_Cox10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.140:FF:000004">
        <property type="entry name" value="Protoheme IX farnesyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016315">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01473">
        <property type="entry name" value="cyoE_ctaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448:SF2">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001773">
        <property type="entry name" value="COX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="9 11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="397"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF88614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="435" mass="48759" checksum="94B5911735EE107E" modified="2022-05-25" version="1">MSFTPIWSRAQNAVVFTTKLRLATRRLMSIRGIKTRNGPKDVHLNTAPIEFTPNIIRNSPKESAIAESARKALSCTTDVSEKSSHIPFEVKSIDPKLRKAKRESLDRSHESSVNLKKLIDPYIQLSKPRLTMLVMLSAICSYAISPNAVSVSQLLSLTVGTTLCSASANAINMGREPDFDRQMIRTQARPVVRGLVTPSQAYKFAALCGTVGSAVLWTGVNSAVALLGASNIVLYAWIYTSLKRRHIINTWAGALVGAIPPLMGWAAASPLTDPGAWCIAGLLYAWQFPHFNTLSHNIRNEYRNAGYVMTAWKNPLLNARVALRYSLLMFPICFGFSYFGVTDWMYQIDSGIINAWMSFWAFKFYWQQNKNYYTRDFGDKAKYNKGLTIANGFAKKTFLVSVLHLPAVLILAILHKKGRWNWLFEDEGKEETLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X6X1</accession>
    <name>A0A8J2X6X1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Altered inheritance of mitochondria protein 32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_15478g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIM32 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X6X1"/>
    <dbReference type="OrthoDB" id="10253744at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03062">
        <property type="entry name" value="TRX_Fd_Sucrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009737">
        <property type="entry name" value="Aim32/Apd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902">
        <property type="entry name" value="ACTIN PATCHES DISTAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902:SF7">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06999">
        <property type="entry name" value="Suc_Fer-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF88661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="326" mass="37291" checksum="939EACCB5B8A6C5F" modified="2022-05-25" version="1">MFIQGRHALRFLVRKVYYHRNYVQINLNTETLAPLSCQCQNYNREINALLPRELQLDTNSPIPRRVPGYKKHVLVLSPQDKSATELEWMSHWQSKLELNPLWPYSIIGKLKSYLKNTKLGSDVLVNAISMQSGSIKAPKRDPHEKAHIFVLPDMKLYKISQSDVEEFAYFLGEGHTKPLADHKLSFTDFLKGADNVTNETDIIPESRSNNEIFEYEDNLADWLLVCGHNQRDRRCGLIGQALIKQISAEGLYKDKNIAVISHIGGHKFAGNVILYNRTGFNKETGLNQLDSLWFARISPPNIIPLLERINAGEIPKEYYRGGLSMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X780</accession>
    <name>A0A8J2X780_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dihydrofolate synthetase</fullName>
            <ecNumber evidence="7">6.3.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN860_06590g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>7,8-dihydropteroate + L-glutamate + ATP = 7,8-dihydrofolate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.12" evidence="7"/>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF88990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X780"/>
    <dbReference type="OrthoDB" id="5212574at2759"/>
    <dbReference type="UniPathway" id="UPA00850"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008841">
        <property type="term" value="F:dihydrofolate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.10:FF:000010">
        <property type="entry name" value="Dihydrofolate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF0">
        <property type="entry name" value="DIHYDROFOLATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001563">
        <property type="entry name" value="Folylpolyglu_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CDF88990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="410" mass="45458" checksum="19ABA3BF7961DA2B" modified="2022-05-25" version="1">MRINLGLERVAKLLAHLGDPQKKLKVLHVAGTNGKGSVCSYLSSILQQGFRVGQFSSPHLVHITDSITVNSKPIAWNVYRDIRRKLETYNSQYMLHCTEFELLTCTAFKYFYDVHCDWCVIEVGLGGRLDATNIFPGACKFSCGITKIGLDHEAFLGTTLTQIGREKAGILVEGVQSAVVDGTNEECVLNVIEEQCERVGCELTITETNPSVIHTRSWSQICPKSPLNGAYQVENLKVALGMLDDLQQQKLINFTEESIQKGLQQVTWPGRLHKLIFHSPDGRSLPLLLDGAHNGSAALELAKFLRAEYGQQPLTFVIAVTQGKKLNPLLTPLIRTQDRVIVTQFGSVEGMPWIHAMKVEELGEYIATLCEDVRTAQCFKDALSTLAKENRPVIVCGSLYLVGEVLRSQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X789</accession>
    <name>A0A8J2X789_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA mismatch repair protein HSM3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_10176g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in DNA mismatch repair in slow-growing cells. Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the 19S regulatory complex (RC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the proteasome subunit S5B/HSM3 family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X789"/>
    <dbReference type="OrthoDB" id="4074002at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12794">
        <property type="entry name" value="Hsm3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040752">
        <property type="entry name" value="HSM3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041335">
        <property type="entry name" value="HSM3_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18794">
        <property type="entry name" value="HSM3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18795">
        <property type="entry name" value="HSM3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein HSM3 N-terminal" evidence="10">
        <location>
            <begin position="11"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="DNA mismatch repair protein HSM3 C-terminal" evidence="9">
        <location>
            <begin position="308"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF88435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="482" mass="55742" checksum="ACD27834D2842CE8" modified="2022-05-25" version="1">MSAEAHSHTRELFDELNNAFESDELPMNINDLLEKSTLDLSTQTSLQEDPKRLLANIKRFLVENLSESQLDHNLLLQLLNSIISICSFEDVLATFSIEDLEEALNSGISPLIEAACKVIAKSYPKGIFANSVLCDILLKLYFDPNADISIITNIEKTIAALSSDELIRRRILINNISLLMAIKKQFEPVSMARLLELLNIEARFMSNGEFDEKLFIIRSEEIFRSLTMDIIMFIHITTFYCKLLEEILLQDQSHNHPNQWLLKHILPILPTFGLIYERRAHYADVKYFARSYLFNLFRKVSYLDDENIFASLDDNYIHISCENEYISDILSFVNPKYVYEKHQHLLIEFSTVMPSHLGILRNFVADEDCFLLVKINLTSSAILAMPYMEQMVLLQRMSQFAHCVRYLLELLPKVMSGLLYGENGEITETECATLRREIFENLLKFDKNVLNVWYEPLREEYKKMTYGGTSNEAQATLASTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X7L4</accession>
    <name>A0A8J2X7L4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="16">ZYBA0S03-12046g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN860_12046g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fungal Na(+)/H(+) exchanger family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X7L4"/>
    <dbReference type="OrthoDB" id="5327978at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000015">
        <property type="entry name" value="Na(+)/H(+) antiporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013928">
        <property type="entry name" value="Cation/H_antiporter_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004712">
        <property type="entry name" value="Na+/H+_antiporter_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032516">
        <property type="entry name" value="Nha1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00844">
        <property type="entry name" value="c_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382:SF4">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08619">
        <property type="entry name" value="Nha1_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="11" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="409"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="14">
        <location>
            <begin position="26"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Alkali metal cation/H+ antiporter Nha1 C-terminal" evidence="15">
        <location>
            <begin position="463"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="domain" description="Alkali metal cation/H+ antiporter Nha1 C-terminal" evidence="15">
        <location>
            <begin position="841"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="485"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="557"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="634"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="696"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="783"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="485"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="508"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="590"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="648"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="700"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="786"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="851"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="863"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="873"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="934"/>
            <end position="944"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CDF89222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="954" mass="106054" checksum="2A655ACE4D6795B2" modified="2022-05-25" version="1">MVWGQLEPTKAHVAYACVGVFSSVFSLVSLFIKERLYIGESMVAGIFGLIVGPHCLNWFNPLSWGNSDSITLEITRIVLCLQVFAVAVELPRKYMWKHWLSVTMLLVPVMTFGWLIIGLFVWIIIPGLNFPHSLLVAACITATDPILAQSVVSGKFAERVPGHLRNLLSAESGCNDGLAFPFIFLSLDLIMHPKQGGEIVKDWICITLLWECIFGCLLGCFIGYVGRIAIRFAEKLKIIDRESFLAFYVVLTFMCAGFGSILGVDDLLTSFSAGAAFAWDGWFSERTKESNVSTVIDVLLNYAYFVYFGAIIPWEHFNDPKIGANIWRLIILAIIVIFLRRIPAVLLLKPLIPDIKSWREAIFVGHFGPIGVGAVFAAITARSQLETQDSHEETPLKELPPEGARHWQIIAIIWPITCFFIITSIIVHGSSVAIITLGRHLNTITLTKTFTTHTTNGNGKTSWMQRLPSLEKNGRSFSLQRVDTEAPSFSGQTAVETSGVPYKPAGGMKRKRKNRRKNRRKELLHALSGNGSDEEELNDLGRERLQREKEARAATFALSTAAEREEKAPLESEGLTPGESDTTEQAVSHEPTKSDVDIMSHDETIHAISGLDQLARDKGNAAIKLLEGGIIHRLGSNKSQNDESPSEAEEKGQRTSEEHSRTSSDAERMRRIREEEEQAHVAYAEDNQVIIENAEGEVLDEARLDKPEKENKRDHHPSSPPSRSDTNRSSGSLHSLKRVLTPPISGIQKFGRSGSRANKYYAYKIDNQLIIEDNDGEVLRRYKINPHSERRKPAEKTKSSSVVGRALSAVGLGSKPGHDGGESGITPSEELNKMVRNPTPAPKQNYRRSEEFEDDDENESGSDEPGTSNSYDDYSDGSDDNDRDNTTSVENSSGDDFETAVERERRLNALGHFSAPRDKDDEEEPPTPMVAPQDTRSSTTQKVKSSIGRKFHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X851</accession>
    <name>A0A8J2X851_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone deacetylase</fullName>
            <ecNumber evidence="3">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_09186g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase family. HD type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3"/>
    <dbReference type="EMBL" id="HG316457">
        <property type="protein sequence ID" value="CDF89643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X851"/>
    <dbReference type="OrthoDB" id="73273at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF14">
        <property type="entry name" value="HISTONE DEACETYLASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="10">
        <location>
            <begin position="20"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF89643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="438" mass="50158" checksum="37881F4A0862FA04" modified="2022-05-25" version="1">MTQFVIATSTFQSQVADLLPCNKWQKSQLTHALINAYSLLGKFDYVIAKPCCRKEDLLKFHSEEYVEVLLNPKYNSLLPYDEDEDSWSQLSEMAFDWATRNNANCHRLKSRAELHNCYQRLQGRKRSAEEAFSNNKGEKLDIQQFNLEGDCPIFSYLPMYCKVVTGATLMLADYINLGEDRTIAINWDGGRHHAMKQKASGFCYVNDIVLTIQKLRAKGFQRISYVDFDLHHGDGVENAFKYSTNVQTLSLHLFEHGFFPTTGSLIDAKKGRGVYNIPLSHGLDDEYLHKVVEELVFPLIDRQDPQLIIIQCGGDGLIGDGYAEWQLSIKGLSRNIIKILNRYQTSSVVLLGGGGYNETVMSRYYTYLTGQLFDSSFLQNEDAVIPEHEFADAYHAEHYKFWAYETEGSLFKKTLRNDNHFDFIEKLKKTYGITAPTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8C4</accession>
    <name>A0A8J2X8C4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BN860_11848g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN860_11848g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316454">
        <property type="protein sequence ID" value="CDF87672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8C4"/>
    <dbReference type="OrthoDB" id="4070119at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014804">
        <property type="entry name" value="Pet20-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08692">
        <property type="entry name" value="Pet20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CDF87672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="451" mass="52302" checksum="A7DB0DDBC076565B" modified="2022-05-25" version="1">MLRATFIPFSSGSRLFITRKRFYSGNSKNGDNKAIMNVSNTQRHTKARLLVPRVPSTDYITAPEIQTEGLFAGYRPLFLGNSPIGEKRSSGPLDNFFTSFANLKLTEDSERVGEVNVQEVIEDLKRDVPSKYMSNSRGRNRKPIIPWDASISGMVYNDEPFKHVPNNVVSKLKPFKIVRIERKSEPKNPRPDMVRMKVHNSKVNDEPEMINIFNAHAKRRKSNGSVWNEGGSLTGDTYETARRKYDREINHFNDKHKFIRSDQRVTKQDIDKLNRLLVKELHKLTNLAVSTDFREMPLPLCIYVDKSIYAKQSLRRTIKKRILDHIQPVLSMTLSAYGTEESAKRFERKMHARIKNTVRDLCECLPSAYFTREAVDCVVGLSPVQGFKRMHWLKPRKRNNVFRGKNFDMDYMFTLDGTFNVTRSGVKYMRYPVTLNCTSFDKVFSEWDYIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8G1</accession>
    <name>A0A8J2X8G1_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Poly(A) polymerase</fullName>
            <ecNumber evidence="13">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">BN860_06282g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Polymerase that creates the 3'-poly(A) tail of mRNA's.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 2 magnesium ions. Also active with manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="13"/>
    <dbReference type="EMBL" id="HG316458">
        <property type="protein sequence ID" value="CDF89987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8G1"/>
    <dbReference type="OrthoDB" id="412748at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.590:FF:000003">
        <property type="entry name" value="Poly(A) polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000002">
        <property type="entry name" value="Poly(A) polymerase alpha, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000001">
        <property type="entry name" value="Putative poly(A) polymerase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.590">
        <property type="entry name" value="Poly(A) polymerase predicted RNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007012">
        <property type="entry name" value="PolA_pol_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048840">
        <property type="entry name" value="PolA_pol_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007010">
        <property type="entry name" value="PolA_pol_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014492">
        <property type="entry name" value="PolyA_polymerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682">
        <property type="entry name" value="POLY A POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682:SF10">
        <property type="entry name" value="POLYNUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04928">
        <property type="entry name" value="PAP_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20750">
        <property type="entry name" value="PAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04926">
        <property type="entry name" value="PAP_RNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018425">
        <property type="entry name" value="PolyA_polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="PAP/Archaeal CCA-adding enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) polymerase nucleotidyltransferase" evidence="19">
        <location>
            <begin position="8"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase central" evidence="18">
        <location>
            <begin position="206"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase RNA-binding" evidence="17">
        <location>
            <begin position="353"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="526"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="530"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="540"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="100"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="233"/>
            <end position="234"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR018425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR018425-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR018425-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF20750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CDF89987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="565" mass="64582" checksum="B7F5E1DA7866D8B1" modified="2022-05-25" version="1">MNYQKIYGITGPVSTAGPTAAENKLNDELIQELKKEGSFESEQETANRVKVLKILQDLAHEFVYRVAKKKNMSDGMARDAGGKIFTYGSYRLGVHGPGSDIDTLVVVPKHVLREDFFAVFDELLRLRPELEEIAPVPDAFVPIIKIKFSGISIDLICARLDLAQIPQSLTLSDPNLLRNLDEKDLRALNGTRVTDEILELVPKPIVFRIALRAIKLWANRRAIYANVFGFPGGVAWAMLVARICQLYPNACSAVVLNRFFKILSEWKWPQPVVLKPIEDGPLQVRVWNPKIYAQDRSHRMPVITPAYPSMCATHNITESTKKVILEELENGIQITNDIFSNKKTWSDLFNKHNFFYKYKFYLTVMASTRGSDEEHLKWSGMVESKVRLLVLKLEALAGIKLAHPYTKPFEAAFTYSNEEESREILDNYGSFKTEEQLNKYQRVTDDNKDTDGIKDKQKCYITTMYIGLDVNVEEKKEKIDIHVPCNEFFNLCRHFNEEYQNSNKYSLVIKYVKQYDLPSEVYELGESRPVKKKSKRRKQDKSAEFPKRAKGETAPGSSIAEATQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8N4</accession>
    <name>A0A8J2X8N4_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_17304g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 12">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
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        <property type="protein sequence ID" value="CDF87912.1"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Zn-ribbon_RPC11"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
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        <property type="entry name" value="Zn_ribbon_RPB9"/>
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        <property type="entry name" value="Znf_TFIIS"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="ZF_TFIIS_2"/>
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        <location>
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    </evidence>
    <sequence length="110" mass="12525" checksum="B7484FDD393BB9DE" modified="2022-05-25" version="1">MLSFCPLCNNMLLVASSDSGVYTLTCKSCPYEFPIEGIEVFDRQKLPRKEVDDVLGGGWDNVDQTKVQCPNYEKCGGESAYFFQLQIRSADEPMTTFFKCVNCGNRWREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
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                <person name="Bigey F."/>
                <person name="Devillers H."/>
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            <dbReference type="PubMed" id="23969048"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1370" mass="148325" checksum="22DE4D95CA81AC91" modified="2022-05-25" version="1">MSAIAFRTPLQPEENTFFGQQFRRLDKEDLGIVTGESLKPLFAASGLPASLLSQIWAIVDTGNKGFLNLSEFSAAMRMIAHLQQRPSLPVTAALYETPAGRLPLLGGGTTTAAAAPAVAPVSSVAAPGTVSPMASSPLRSTSSNLSAIPLMSHQDVSKFSQLYDRTAGNASSLPGDKAKEIFMKARLVTNTLGDIWALCDRNVSGSLTKQEFVMAMYLIQLCMARHPSVTPLPSSLPAQLWNSVNSSTMGSSASGGVLGGGVTPLSANSTGRTLSRQNTLQRLSSGVFNNASTDWSLNFEKKRQFDAIFDNLDKSHTGSLGPQVLVNFFLSSRLSQETLASIWDLADIHNNAEFTKVEFAIAMFLIQKKNAGVELPDVIPDQLLHSPALGLFPNQQPPMGVPSRETKPSFSEQPQAPVQVPQHSNNGSLNDLLALNNSFTSPSPPQQPPLRQTSSFSRDSTGGSIPVNSYGHNSGGANRNFTPQMDMGHNVIREEAEENLTHQQYNQQPSVQKNVIPQSSQRGVNSMSNYASPNLPSANLAGGGTAGSTGAAGLRENNDLYADAEASAQLSYATTEVANMSNQVKSLTKQAGITSDKKTRAAQELKRVNATKASIEKKLSDLRASHDQNVKQVEQLEAQLSEANKETAALTQQLTVTEGNYHAIESKVSELSQSYQEAQQKNAELRAQISKLNTMSSTLQTQLAEKQRQVKQERSMVDVNTRQLELNDVTVANFEKEINGLGEKLQVYLNKRKELDDYQKTVEQQHSQLQSRYQQLETRNQDLSVRGQELEQRRKALREQEKAYQQQAAQLQNMFEELNKKKASYPAAVREVTASRTINYTSKSPQTEGVATNARGVKVGTHEDEVSKFVEATVANSKLGGNEDEERAESDVFDKDVPTVGSQTEADDESETRRARDRVENITDRFEGDLNEYGIPRTQSLTSSVANNAPQSVVEDVELPETFQENTTRATANRDSVIPGQWGGSVNGGNGIPEESIHSSSQYGDRENNLTQRITQERSESPTERDVEGRYKNIDEEFPPIQELNVNESDSSSDSEVYQDTSEDAAPRYTGVAGGQYSSQSKIPTPISSRENPASKATEPPLQGSQASLDPSERSSQGSHPTAPGSQAPYGSTGSVLQDKGSLPPMNNASSYPGGGSAPRLDNATNSSLFPDRSVSNPSLPAAGPPVGTVSTGSGYRDEFDDEFAGLEQADEEAPDSMDEAQMNNGSMEEFEKIEHQDLDDELQQNAFTDSQVTGGRQPVQSQNNSGVSNDEWDEIFAGFGNSKPYAIKNSADSPAVTGGAEDPVENPLPVRGPLEKTETPVKRSIATTPRSLAVEELSGMGFTDKEAVDALEKCKWDLEAATNYLLDSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X8X5</accession>
    <name>A0A8J2X8X5_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="13">BN860_03752g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN860_03752g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-5/BimC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X8X5"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000073">
        <property type="term" value="P:initial mitotic spindle pole body separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01364">
        <property type="entry name" value="KISc_BimC_Eg5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047149">
        <property type="entry name" value="KIF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047241">
        <property type="entry name" value="KIF11-like_kin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031852">
        <property type="entry name" value="Vik1/Cik1_MT-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970:SF19">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16796">
        <property type="entry name" value="Microtub_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="12">
        <location>
            <begin position="44"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1074"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="446"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="731"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1074"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1105"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="132"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CDF88163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1135" mass="129216" checksum="AA673E75B3F87EF3" modified="2022-05-25" version="1">MSDGYSGDSKRDPSRLHSLSRPSSGHSSSPATQDPHQRHQIGSNIKVYVRCRSRNQREIDEKSSVVVSTMGRKGKSVILSNPSSPLSHPKKTYTFDEVFGADSDQESIFSEVARSYIQEMLEGYNCTIFAYGQTGTGKTYTMSGDMSIWGDLESQDKILLGEHAGIIPRVLVHLFKQLSEETTEYSVKISFLELYNERLKDLLSDSNSEEDTIRIFDNNNSSGHVSGSSHMKIQKSKSRATSVSCATVPLSSSSVNTSSSSSSITVKGMEERYIKSAYEGLQLLTKGSMKRKVAATKCNDLSSRSHTIFTIVTHIARTDPVSGKQYVKIGKLNLVDLAGSENINRSGAENMRAQEAGLINKSLLTLGRVINALVEHSHHIPYRGSKLTRLLQDSLGGKTKTCIIATISPARISMDETVSTLEYATRAKSIKNTPQVNQSMSKDSCIIEYIHEIERLRQELKASRQKEGMYITQEQYDLYESNSILVEEQKVVIQNMGEQIKRFKEKYVEQTELSKEAQRQLKDSQRMCDTLRAQKNFILETFEKYHNTCNDHAAKIEHIHEGNIALMDALKVERDEMSTSALNNADTIDKILSTLVERSNVLTELKSNLETHTRNFQQMSAKVLLDFETEALSHIMQSKTELSSIKMDDFIERIQTMHSSIMHTLHYISKPWKKDIEGIYFTHKHILENCGKQLCEYIDELENSFLVMWKKLKGMAISEVNQLQFANDRSFEGVRNLINEERKRSQFLENELLIKQRALKESNEQLEKLEMYFKNHLTRERRDILSDIVKAIANSEKRHRMLDEQMFEKSSSILNKTKEISHAQYVSDICHLNRQHNESLTTVDDLLGTFKTDFKELSQEKSMQLERSIKYQSIKDSFQEFLPKLSKDCTEYSSKHLLKLIRDLDMEISAAAESLGNEITSIVNEARQNILCDVKEDETHLRAALENLTNLSDFITEIRKQKAPQHFQKHSNVLETHCTDLQSVILDLKKSNEYLTSAKRPKIGVDLEKCVIRKLPRLDGPVDYSSYKDYDICRYGEKGQNGDVYTHGSIFNPSTPMPVPDQPLPKVLVPKSINSSTKRSHTLPSESRKSYLNINAGGNNLKRRFTLDPPEKTDKENIDDSQMVKKTRNTTNVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X918</accession>
    <name>A0A8J2X918_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ZYBA0S07-01222g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_01222g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxisomal targeting signal receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X918"/>
    <dbReference type="OrthoDB" id="10006023at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005052">
        <property type="term" value="F:peroxisome matrix targeting signal-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024111">
        <property type="entry name" value="PEX5/PEX5L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130:SF0">
        <property type="entry name" value="GH08708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR PEX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="411"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="445"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="157"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF90447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="562" mass="63438" checksum="A8939110906D5A4A" modified="2022-05-25" version="1">MNAADCSIGNNPLAQFNKHAQNDKSLQHGSSLIRTPEPTAQQFKSSQNSMSDTAKFHMNNFMSGASPSNFQMRIGQQPKALSGSSSNAIMSSSVRELPGTPLQGNTWSQEFQTKEVPQMEHVPQQVGGPSYHYRGMGMQSMPMVTGPRMMMKPSIMSQQQQLSPQAADQSESWDEQFKVLEREVAENLHLQEKQDNGQKLEEGSVNQEDVVVEDQYQSEFQEVWDSLQKDSDDLLTEELSAEHGWENDYRRFLNGKDSGNAQYKFEGDNQFLHNPNAYEIGCLLMENGAKLSEAALAFEAAVQESPQHVDAWLKLGQVQTQNEKELSGINALEQCLKLDQHNVEAMKTLAISYINEGYDVSAFTMLNRWVEAKYPEMVEGVMEIEPERYRLNIRVRERFLHIANKLPQVDPDVQLCLGLLFYADDQFDKTIDCFKAALSVNPNDELMWNRLGASLANSSRSEEAIQAYHRALQLKPSFVRARYNLAVSSINIGCYREAAEHLLTALSMHEVAGASVNPSMNVDSNNILETLKRAFIAMDRRDLLEHVKPGMELNQFRGEFSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9A4</accession>
    <name>A0A8J2X9A4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="14">BN860_10924g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_10924g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316455">
        <property type="protein sequence ID" value="CDF88466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2X9A4"/>
    <dbReference type="OrthoDB" id="3970464at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000019">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000024">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF30">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE ISOZYME 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036565">
        <property type="entry name" value="Pyruvt_ip_decrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="13">
        <location>
            <begin position="14"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="11">
        <location>
            <begin position="210"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="12">
        <location>
            <begin position="410"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="566"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="566"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="453"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF88466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="587" mass="66061" checksum="A680F83AB9918B2C" modified="2022-05-25" version="1">MHYTEVENLATTITIADYLFHRLKQLKVNTIFGLPGEFNAPLIDKLYSIQGLRWAGNTNELNAAYASDGYARLKGLGCIISTFGVGELSAINGIAGSYAEHVGVLHVVGMPPTSAQTRQLLLHHTLGNGDYTVFHRMATEVSCHSAVIVDTDFCAQEVDACISKAFTLQRPVYMGIPVNLVDMPVESHRLNQPLDLSVPPNDPNKENEVVEQILSHFYKAKHPVIIADACVTRHAVDAETRELCERTQFPLFVTPMGKGAVDESLPQFGGVYTGSISSPSVREVVGFVDFLIVIGCMLADFETSSFHFGYRAKELVLLYPTLVKFKHATYPDLNIKPLLQSLLSQLRESQLSYHTQPRQSMVIPKLELPSTHSLRQEWVWNQISQWFQERDIIITETGTSAFGINQTHFPHHTLGISQALWGSVGYSLGACLGAAMAATELSKTHRVVLFIGDGAFQLTAQELSTMIRWRLTPYIFIMNNQGYSVDRFLHSNASYYDIQPWDYSRLPEIFGAQDYESRKIVTVGDFQSMVKDPNFANAERIRILEIMLPPMDVPQALRERWVQDEKRELERRESVESVKRQKLLPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2X9F4</accession>
    <name>A0A8J2X9F4_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ZYBA0S07-04808g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN860_04808g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="27683at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003980">
        <property type="term" value="F:UDP-glucose:glycoprotein glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040497">
        <property type="entry name" value="Glyco_transf_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009448">
        <property type="entry name" value="UDP-g_GGtrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040694">
        <property type="entry name" value="UGGT_TRXL_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040692">
        <property type="entry name" value="UGGT_TRXL_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11226">
        <property type="entry name" value="UDP-GLUCOSE GLYCOPROTEIN:GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11226:SF0">
        <property type="entry name" value="UDP-GLUCOSE:GLYCOPROTEIN GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18404">
        <property type="entry name" value="Glyco_transf_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18401">
        <property type="entry name" value="Thioredoxin_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18402">
        <property type="entry name" value="Thioredoxin_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035297178" evidence="7">
        <location>
            <begin position="20"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="domain" description="UGGT thioredoxin-like" evidence="8">
        <location>
            <begin position="254"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="UGGT thioredoxin-like" evidence="9">
        <location>
            <begin position="408"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Glucosyltransferase 24 catalytic" evidence="10">
        <location>
            <begin position="1073"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1316"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1316"/>
            <end position="1328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CDF90600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1335" mass="153569" checksum="3058364809F6AD38" modified="2022-05-25" version="1">MKIYLAFFTVLLYLKAVQCVLLSSIGTSVPESAKIWSIASYLLRNCNENILQSIYPMILQLDDETEDSLGDSLCYTVAKLISDLGEPHLASLMPLYCKWYPMGNQDPHERYTYVPNELYFILNGKKYENPDDVYYLKSGELKKQAQLPDSKMLAPGEVVIGNNREAPIVHFYGCPDEKGVFQEFNRNLFSEATQTGKLRFIWRSTCSLEKTALGPFPVALTTKEQSNLENLPQEILKIPESFDKSKYDLLTPREDEFTNVDLKAAKLIADHYQNNQNFDAAFLYAKDFVNNFPILVSELLSLSGDTDVISKSNDELANKGVDHNLLGLYINGQNWKVSSLNEVTLLDALEAELHKLRQLSFSLSKIDNRVSLLTAKNLINDFARISLPNLQQSQPVKLDLHRIRGFSESVIYFNDIEIDDQYHDLSQDIEKFFEKSKFGEIPEYRHNWSELIFVIDFNNLEDPDTKLALEGLQRAISVITQGYPQRIGLLPLNTGEAESIIKKIYHLRDKDLLLLEDFLKDLPIGRGRISADYQNTPDTAKLLRNLQIDQTSIIINGEIYPFRKNTWHYLVAKTIKKDTGFLKRELSKYIKHDEGKVRPMIDVRGILHLKSSETRHKKYTPDYFADSTYTAMDNTVLDVWNDRLCEYFKGKEYNVLHTITIVDDFNTVQALKRLYNLVHVKFVGIRFRAIHTGILSSTWNKVKDSSSKSSFNSILKSIIKKCSHYEPKYQLDDIVLQSWLPDLTPLFFSAKSFMIINGRFLHFEIDEIPQTKLFEATIKREAQRTLDTLEALEISYPGFSEKKIDSELVESISAILTKMFYQGTDIYHNGPDYSTETSLSRIDLTKILEINNFTIFQNVNHELRPIDLVLIVDPLEERTQKFYSLLEEVKGLSFVNLKIVLYPTKDLKIMPINRIYVQDFKNELGSKLPELSKIFDVEIEAPSNLAITSNQRVIGYVIEVHAFDKDDAVSEGVVDGIGGVCLELVDAKENVVDQTITMKTFGYAQFIAPQLWCQYYVRSCDPQYKVISFSSDARSDYLPVKSFDLKNFNPIKVYVQLEKTAAPVISAERENIINIFTVLKNDIAAEGNYKKMVLSILLSPQRHQQDVKFWILDQPYLSASLKKFIEDVNSDPQLDAKIELIQYKWPSWLRPQRFIARRIDALKILLLDVLFPQTVQKVFYMDPEISQLPDPFKLNEKITTKLPFAMFKMVGHGYWETGYWAKRIGEHNLKFHSVEPMFAINLKKFRDLDGGDKLRIHYQRLSADANSLAKVDQDLINDVQEEVPIRTLSRSTIKYLKTDPHVIEAWLEKKEAGTSLSGDDTELLSESNEVLHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XA59</accession>
    <name>A0A8J2XA59_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ZYBA0S03-10308g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN860_10308g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316456">
        <property type="protein sequence ID" value="CDF89149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J2XA59"/>
    <dbReference type="OrthoDB" id="47330at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19499">
        <property type="entry name" value="RecA-like_ClpB_Hsp104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000017">
        <property type="entry name" value="ATP-dependent chaperone ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000120">
        <property type="entry name" value="ATP-dependent chaperone ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000025">
        <property type="entry name" value="ATP-dependent Clp protease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000010">
        <property type="entry name" value="Chaperone clpB 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050130">
        <property type="entry name" value="ClpA_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638:SF176">
        <property type="entry name" value="HEAT SHOCK PROTEIN 78, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="131"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="529"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="Clp ATPase C-terminal" evidence="9">
        <location>
            <begin position="702"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="343"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CDF89149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="807" mass="90889" checksum="6321B2970B97E376" modified="2022-05-25" version="1">MLRRIKRSPLERQLQSRIASIIQNSATVTPVVTRVSSPTKSTSNDLRQVISRRSLMTDALTRTSVRFPPGPTISKRFIQMKMNPNQGEQEKPALEQFGTNLTKLAKEGKLDPVIGRDEEIARAIQILSRRTKNNPVLIGRAGVGKTALIDGLAQRIVAGEVPESLKDKDLVTLDLGSLIAGAKYRGEFEERLKKVLDEIDKSNGQVIIFIDEVHMLLGLGKTDGSMDASNILKPRLARGMRCISATTLDEFKIIEKDPALTRRFQPILLNEPSVGDTISILRGLKEKYEVHHGVRITDTALVTAAVLSNRYITDRFLPDKAIDLVDEACAVLRLQHESKPDEISKLDRAIMRIQIELESLKKETDALSVERKEALQKELDKKNDELSRLTKVWENERAKIESIKTAKADLEKARIDLEKSQREVDYAKASELRFAVIPELERKVALSEKKNDPNEVNLLHDSVTSDDISKVVAKMTGIPTETVMKGDKDRLLYMENSLKERVVGQDEAIKAISDAVRLQRAGLTSEKRPIASFMFLGPTGTGKTELTKALAEFLFDNESNVIRFDMSEFQEKHTVSRLIGAPPGYVMSESGGQLTEAVRRKPYAVVLFDEFEKAHPDVCKILLQVLDEGKLTDSQGHVVDFRNTIIVMTSNVGQDILLADENVGEDGKVSEKTKTKVINAMKKLYPPEFINRIDDLLVFNRLSKKVLRSIVDIRLEEIQERINEKRMKLDLTDEAKTWLTDHGYDPLYGARPLNRLLYKSLLNPMAMYLLKGQLRNEETVRVVVENGKLVVKPNHKEGEVVEEEPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XAT2</accession>
    <name>A0A8J2XAT2_ZYGB2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin carboxyl-terminal hydrolase 2</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Deubiquitinating enzyme 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Ubiquitin thioesterase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ubiquitin-specific-processing protease 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN860_01750g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="HG316466">
        <property type="protein sequence ID" value="CDF91707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2420415at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061136">
        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02666">
        <property type="entry name" value="Peptidase_C19J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000176">
        <property type="entry name" value="Ubiquitin-specific protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044635">
        <property type="entry name" value="UBP14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025305">
        <property type="entry name" value="UCH_repeat_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982:SF6">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13446">
        <property type="entry name" value="RPT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="13">
        <location>
            <begin position="730"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="853"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CDF91707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1301" mass="149540" checksum="5A9FF3864CBE03BA" modified="2022-05-25" version="1">MLLRDESTESANHGSTHATSPNLAPQLETEQIDELMSESEASEETDKQTIQETQDSGKVFLYPSIAAHFPFKTADRLLDDVVHDLSFINSTEHEILYSDLHAGILKSPVLPYSRKIGKLRSYNLGSLVDQVALQTKFEYDSVSCPQHNHLQVFMGVLIDVTQQNVATFDNLTTLPIFHLKVTVKTRDILERTRKRAGITHYHIVDEEELHPYDKQDLLTFDAKDPQLVDYAIYVSSDANKLILIEIFKPEFNSLEKQQAFTEDSIAQRYERACEKFDSLDPSKVPTQPDCINTLFKIFKGPLNRKNSTDPRKTINPNNVSLNSHIDPSWLTSKYGFELRVEKDEETGEEILEYAPPDLTDYHRDYKVRKLRESFTRKCLELIFLGRVSIRLLSEEEINKTPKTFRIFSMMQTNFSTMFWFQLLGEYRAIFNTESNSPLDADHHFIDLCSSYNYIDKDIIRNYEVQCSLDPDNVGVYVDALNFIANRKGAYQLIAYCGKQDVVGKEALDHALSAFQIDPKEVDPHSLSDTVLLSIYKSETSATRNQNRHVDLKNSLRLLAKFKKSPKLKFYADYEPYRSLAQAYDVLEVDESVDDDIIQTAYSVKINDSPGLKLDCDRALYTVAVNTRSLTLFNFLMQQCDEFQIYFGPDQFTYQDALTVLQVNENAGDDTILEVFQRKWHKEPIFDPDQFLKLKSALTKIAIERNSKLILHFIDTGTVDASCLPVENWPTGLNNIGNTCYLNSLLQYYFSISSLRQYVLSYRKTVNDLIKNPEYASRRIGGREITESEVERSVQFVYQLRDLFYSMIHAQERCVTPRKELAYLAFVPSGLGVEFELPRESQDNGNSLEKEKFINIDSEPSPPPNDEPLLIDLDDDNNGNSNHDSDEIMVENDNNAADDIKANAKEKVGHQDIDVLMTDNLLPKDGAECHRDGSPPNVAEQVAIMNTSTRVAKISSDQLENALEMGRQQDVTECIGNVLFQLESASDPISLDNDNEQNDLVKQLFYGNIKQNIIPLSDETRIRTKYERFLSLLVNIGDHPKDIYDALDLYFRDEYLSLEEYGNVKRTVAVTDFPTILQIQIQRVYYDRERFMPFKSIDPLPFNETIYMDRYTDMNDPVLLRRKEETQKMVLELSALKKRQSELLSKNDLGLSRKGALIETSNFLKSGVLESQGIHVENQSALAEQLEKIVSGVDEELSQLYHKITQLEGQISHQYDEFKNIGYSLFAVFIHRGEASYGHYWIYIKDRFKNGIWRKYNDETITEVPESEVFNFAEGNTATPYFLVYVKQGKEQEIEPLKRVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J2XC80</accession>
    <name>A0A8J2XC80_ZYGB2</name>
    <protein>
        <submittedName>
            <fullName evidence="15">ZYBA0S07-04566g1_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN860_04566g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Zygosaccharomyces bailii (strain CLIB 213 / ATCC 58445 / CBS 680 / BCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227)</name>
        <dbReference type="NCBI Taxonomy" id="1333698"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Zygosaccharomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E0060613" last="E0060613">
            <title>Genome sequence of the food spoilage yeast Zygosaccharomyces bailii CLIB 213(T).</title>
            <authorList>
                <person name="Galeote V."/>
                <person name="Bigey F."/>
                <person name="Devillers H."/>
                <person name="Neuveglise C."/>
                <person name="Dequin S."/>
            </authorList>
            <dbReference type="PubMed" id="23969048"/>
            <dbReference type="DOI" id="10.1128/genomea.00606-13"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CLIB 213 / ATCC 58445 / CBS 680 / CCRC 21525 / NBRC 1098 / NCYC 1416 / NRRL Y-2227</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EMBL" id="HG316460">
        <property type="protein sequence ID" value="CDF90589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000019375">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15480">
        <property type="entry name" value="fMyo2p_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01380">
        <property type="entry name" value="MYSc_Myo5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046943">
        <property type="entry name" value="Fungal_Myo2/2A_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036103">
        <property type="entry name" value="MYSc_Myo5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF706">
        <property type="entry name" value="DILUTE CLASS UNCONVENTIONAL MYOSIN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50084">
        <property type="entry name" value="Myosin S1 fragment, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="14">
        <location>
            <begin position="4"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="13">
        <location>
            <begin position="75"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="Dilute" evidence="12">
        <location>
            <begin position="1233"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="625"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="9">
        <location>
            <begin position="658"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1097"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="968"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="625"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="169"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CDF90589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000019375"/>
        </source>
    </evidence>
    <sequence length="1590" mass="181625" checksum="F0C8A8D1A37B8573" modified="2022-05-25" version="1">MSFEVGTRCWYPSEKEGWIGGEVTKYEVKDGKYHLELTLEDGSVVPIVTDSLVREGSDESGNQKLPQLRNPPILEATEDLTSLSYLNEPAVLHAIKQRYSQLNIYTYSGIVLIATNPFDRVDQLYSQDMIQAYAGKRRGELEPHLFAIAEEAYRMMKNDKKNQTIVVSGESGAGKTVSAKYIMRYFASVEEENSNAMDNVQHQVEMSETEQKILATNPIMEAFGNAKTTRNDNSSRFGKYLEILFDKNTSIIGARIRTYLLERSRLVYQPKIERNYHIFYQLLAGLPQEFKEELQLTNADDYLYMNQGGETQIAGVDDAQEYKTTVEALTLVGVNKATQSQVFKILAALLHIGNIEIKKTRNDASLSSDEPNLKIACELLGIDSYNFAKWITKKQINTRSEKIVSNLTYPQAVVARDSVAKFIYSALFDWLVENINTVLCNPEVSDQVQSFIGVLDIYGFEHFEKNSFEQFCINYANEKLQQEFNQHVFKLEQEEYVKEEIEWSFIEFNDNQPCIDLIENKLGILSLLDEESRLPAGSDESWTQKLYQTLDKPPTNQVFSKPRFGQTKFVVSHYAHDVAYDVEGFIEKNRDTVSDGHLEVLKDSKNETLQGIIKNLENAAARLEEAKKAQQEQNKKRPGPARTVQRKPTLGFMFKQSLIELMDTINSTNVHYIRCIKPNSEKEPWKFDNLMVLSQLRACGVLETIRISCAGFPSRWTFSEFVLRYYLLIPSNEWSQILGSKGPTEDSVVQICKMILDSTVTDKEKYQIGNTKIFFKAGMLAFFEKLRSEKIRNSSVLIQKNIRAKYQRKQYLAIQRSLHLIGACSLGLIVRRRTRDQFMTKAAIMVQAMFRGHRTRSRTLSVLDSVVRIQSLVRQRLSVKEMKAKQESNAAVTIQSRVRSFRPRNSFNRSRKSVVKVQALVRRRSAMAKLKALKSEAKSVNHLQEVSYQLENKVIELTQNLASKVKANKEMTARITELQAALAESANLQSIIEGQKREHSKALEKQAMAHDHVYKDLEDQRKEAELQMEQVKQEVQSMTEKYNKIKEESRAQLEELENTRNLLNDSKTQNSYLHDQVKTLKEEISRLQNHVASSATVYAQTPRSSKRYSMNGSDTMSPGQKNLISMNGPSTTVPVTGLGIENGTAISGGTGDNKSTMSTLSQINDELYRLLEDTKVLNEEVTEGLLKAFKVPDSGVAIQLSEREVLYPARILIIILSDMWRLGLTKQSEGFLAEVLTTIQKVVQGLKGADVIPGGAFWLTNVRELYSFVIFAQESILNDESYNKGLTEDEYKEYVVLVTELREDFESLSYNIYNIWLKKLQKDLQKKAVPAVILSESLPGFKNEPNRFLSNLFGSGPEYTMDDILTFFNSIYWCMKSFHVENEVFRDTVLILLNYVDAICFNDLIMRRNFLSWKRGLQLNYNVTRLEEWCKTHYIPEGADCLQHLVQTSKLLQLRKQDLDDIKLLCEICTALKPAQMQKLMSQYAVADYEAPISGEILNYVAEKVKKGASLSSDGKSKVHSDDIFLQTETGPFEDPYVGMETRQFRKIEAYIPAWLNLPTTRRVVELVTRQVSVQESNSLFDSTDKEENP</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>